Background: Antimicrobial resistance (AMR) is a growing global public health concern associated with the overuse and misuse of antimicrobial drugs, including antibiotics. Self-medication with antimicrobials is a common practice that can contribute to AMR. This study investigates the prevalence of self-medication with antimicrobials among dental healthcare students, their behaviors, and perspectives on this practice. Methods: A cross-sectional study was conducted among dental students in Chennai, India. A structured questionnaire was used to collect data on self-medication practices, knowledge, and attitudes related to antimicrobial drug use. Data were analyzed using descriptive statistics. Results: Of the participants, 61.3% admitted to self-medication with antimicrobials. While 76.8% correctly identified antimicrobial drugs, 47.4% believed these drugs should only be used with a doctor's prescription. Views on the relationship between AMR and self-medication varied. Knowledge of synergistic antibiotics and sulfa drugs was mixed. Commonly self-medicated antibiotics included Penicillin (28.6%) and Metronidazole (12.1%). Participants had mixed opinions on self-medication for self-healthcare, with 47.2% considering it a fair practice Conclusion: This study reveals a complex landscape of self-medication with antimicrobials among dental healthcare students, with varying levels of understanding, practices, and awareness. Targeted educational initiatives are needed to promote responsible antimicrobial use, reduce unnecessary self-medication, and address knowledge gaps to combat AMR effectively.