Mist Nets Research Articles

Trypanosoma cruzi in domestic and wild mammals in the northeast region of Colombia

Trypanosoma cruzi hosts can serve as a source of infection for animals, vectors, and humans, contributing to the establishment of Chagas disease (CD) in a given area. Traditionally, the Department of Córdoba has not been considered a transmission area for CD; however, the report of several acute cases of Chagas disease highlights the importance of studying the dynamics of disease transmission in this region. This study aimed to detect T. cruzi in domestic and wild mammals in the department of Córdoba. In 2017, a cross-sectional descriptive study was conducted in six villages in two municipalities in the department of Córdoba. Blood samples from dogs living in the zones were collected in EDTA vacutainer tubes for domestic mammals. Wild mammals were collected using Sherman and Tomahawk traps and mist nets in crops and peridomiciles. T. cruzi DNA was detected using the kinetoplast DNA (kDNA) variable region and the tandem repeat satellite region of T. cruzi as molecular targets. We sampled 168 dogs and 146 wild mammals. The detected prevalence of T. cruzi was 6.37%; the TcI lineage was found in D. marsupialis, H. anomalus, and one canine. A specimen of D. marsupialis with TcI and TcII lineages was also identified. T. cruzi DNA was detected in domestic and wild animals in the study area, indicating the circulation of the parasite in peridomestic environments. D. marsupialis may represent an important host in maintaining this region's wild and domestic cycle.

Multiple dimensions of phyllostomid bat biodiversity across ecosystems of the Orinoco Llanos

AbstractUnderstanding the impacts of habitat conversion on species assemblages across multiple biodiversity dimensions (taxonomic, functional, and phylogenetic) and spatial scales is pivotal for implementing effective conservation strategies. Here, we surveyed phyllostomid bats using mist nets in riparian and unflooded forests, flooded savannahs, and conventional rice fields to investigate how changes in habitat quality affect multifaceted diversity from two Colombian farming systems in the Orinoco Llanos: traditional farmlands with high‐intensity agriculture (mainly rice production) and Civil Society Nature Reserves with greater ecosystem protection. We used a unified framework based on Hill numbers for quantifying bat taxonomic, functional, and phylogenetic diversity and modeled the relationship of these diversity facets with landscape variables (habitat cover and patch density) across three spatial scales (0.5, 1.5, 3 km) using Bayesian generalized linear mixed‐effect models. Our results indicate that increasing human activity toward rice monocultures representative of traditional farmlands negatively affected all diversity facets. In contrast, forested habitats associated mainly with riparian forests within private reserves contained higher taxonomic, functional, and phylogenetic diversity than savannahs and rice fields. However, the differences between riparian forests and rice crops were significant only for phylogenetic diversity, indicating loss of evolutionary history after habitat conversion. At the landscape scale, forest cover was a significant predictor for functional (0.5‐ and 3‐km scale) and phylogenetic diversity (0.5 km), and bats responded negatively at the 3‐km scale to rice patch density from a functional diversity perspective. Increasing habitat quality through preserving forest cover and patches should minimize the harmful effects of habitat conversion on multidimensional bat biodiversity. Furthermore, the conservation of riparian forests and the creation of more wildlife‐friendly farming, as practiced in the reserves, should be prioritized to ensure high levels of bat taxonomic, functional, and phylogenetic diversity across Orinoco countryside landscapes.

New distribution and breeding record of Wroughton’s free-tailed bat (Otomops wroughtoni) (Chiroptera: Molossidae) from Meghalaya, India

Abstract The Wroughton’s free-tailed bat (Otomops wroughtoni) is classified as Data Deficient under IUCN Red List due to inadequate information on its distribution, population, and ecology. The species was previously recorded from Belgaum district of Karnataka and from East Khasi Hills and Jaintia Hills districts of Meghalaya and from Cambodia, represented by a single specimen. Through our study on Chiroptera taxa across 19 Community Reserves between October 2021 and January 2022 using physical search, mist netting and acoustic study we have identified a new colony of O. wroughtoni from the Mikadogre village in South Garo Hills District of Meghalaya, which extends the current distribution to 135 km from the nearest known distribution site. Interestingly, unlike other known roosts of O. wroughtoni which are in large caves with high roofs and deep crevices or cupolas, this roost was in the open limestone cliff located next to a stream. In addition to this, we have also documented the breeding colony of the species from a previously known site at Pynurkba. This new finding emphasises that there are more potential roosts across the Northeast region of India with similar habitats and altitudes waiting to be identified.

Importance of riparian vegetation and wood-pastures in the maintenance of bat assemblages in a highly fragmented landscape in Veracruz, Mexico

Abstract To investigate whether trees and palms scattered in pastures contribute to the maintenance of bat assemblages in landscapes highly fragmented by livestock farming activities, we evaluated the bat assemblages and foraging guilds between wooded pastures and riparian vegetation. Using mist nets, we captured 1013 bats from 29 species and four taxonomic families. We found that the abundance, richness, and composition of bat species were not significantly different between vegetation types. Frugivores were the most abundant guild, and their abundance did not differ between vegetation types, indicating that wood-pastures allow the presence of these bats in the landscape. On the other hand, insectivores were more abundant in riparian vegetation and, interestingly, nectarivores were more abundant in pastures, likely due to the great plasticity of species of the genus Glossophaga. Our results are encouraging, since they demonstrate that a rustic pasture management that promoting the presence of scattered trees and palms along with belts of riparian vegetation, allows the maintenance of bat assemblages in a highly fragmented landscape.

Preliminary checklist on species composition of small mammals and birds in Marine Research Excellence Centre (MAREC), Pulau Tuba.

Located in Langkawi archipelago, Andaman Sea, Pulau Tuba is one of the islands that is densely protected as a forest reserve. We investigated and documented the species composition of small mammals and birds at two different sites in the forest of UiTM Marine Research Excellence Centre (MAREC). Wire cage traps were used to capture non-volant small mammals while mist nets were used to capture bats and understorey birds. A total of 35 individuals were captured consisting of four species of small mammals and four species of birds. Among the most abundant species captured were Rattus tiomanicus, Leopoldamys sabanus and Cynopterus brachyotis. The calculated Shannon diversity index was 1.58 indicating a low diversity of small mammals and birds within the area. Our current study stipulated that few individuals were caught due to the rainy season affecting Pulau Tuba during the study period. Further surveys and studies should be conducted for a longer period in different seasons so that more species of fauna can be documented.

Unraveling the diversity of Trypanosoma species from Central Mexico: molecular confirmation on the presence of Trypanosoma dionisii and novel Neobat linages

Bats are one of the groups of mammals with the highest number of associated Trypanosoma taxa. There are 50 Trypanosoma species and genotypes infecting more than 75 species of bats across five continents. However, in Mexico, the inventory of species of the genus Trypanosoma associated with bats is limited to only two species (Trypanosoma vespertilionis and Trypanosoma cruzi) even though 140 species of bats inhabit this country. Specifically, 91 bat species have been recorded in the state of Veracruz, but records of trypanosomatids associated with this mammalian group are absent. Due to the complex Trypanosoma–bat relationship, the high diversity of bat species in Veracruz, as well as the lack of records of trypanosomatids associated with bats for this state, the aim of this work was to analyze the diversity of species of the genus Trypanosoma and their presence from a bat community in the central area of the state of Veracruz, Mexico. During the period of January to August 2022 in the Tequecholapa Environmental Management Unit where bats were collected using mist nets and blood samples were obtained from their thumbs. We extracted genetic material and amplified a fragment of 800 bp of the 18S ribosomal gene of the genus Trypanosoma by conventional PCR. The positive amplicons were sequenced, and phylogenetic reconstruction was performed to identify the parasite species. A total of 285 bats (149♀, 136♂) belonging to 13 species from 10 genera and a single family (Phyllostomidae) were collected. Twenty-three specimens from six species tested positive for the presence of Trypanosoma dionisii, Trypanosoma sp. Neobat 4, and a potential novelty species provisionally named as Trypanosoma sp. Neobat 6. The results of the present work increase the number of species of the genus Trypanosoma infecting bats in Mexico and in the Neotropical region.

Exceptionally Uniform Bat Assemblages across Different Forest Habitats Are Dominated by Single Hyperabundant Generalist Species

Woodland bat assemblages are usually structured in a space according to the distance from the ground, water, and obstacles, features that often define chiropteran hunting tactics. Consequently, the bat species composition differs strongly among various habitats, even within the same forest patch. However, when conducting local bat surveys in Wolin National Park (WPN), we revealed an unexpected uniformity in the qualitative and quantitative structure of bat assemblages, based on mist netting and ultrasound recording. In total, 10 vespertilionid species were detected. Across all methods and sampled habitats, a single species, Pipistrellus pygmaeus, predominated, while no Barbastella barbastellus, an old forest specialist, were detected, despite the abundance of their preferred daily roosts. We also reviewed the literature for mist-netted bat samples in four different habitats in lowland Polish forests. The samples usually clustered based on habitats, and the same habitat classes often clustered very closely despite representing geographically distant forests. The exception was WPN, where all four habitat classes formed a tightly packed cluster. We hypothesize that P. pygmaeus might act as a hyperabundant native species, a successful generalist that reduces the contribution of more specialized taxa in the assemblage. It probably benefits from both forest renaturation and anthropogenic cross-boundary subsidy.

Chewing lice (Insecta, Phthiraptera) and ticks (Arachnida, Ixodida) associated with birds in highland marshes of the Brazilian semiarid

ABSTRACT In Brazilian semiarid, highland marshes are home to a great diversity of species; still, these ecosystems suffer constant impacts due to agriculture, logging, and livestock. Here we describe a community of birds and their ectoparasites in anthropized remnants of a highland marsh ecosystem, located in Northeast of Brazil. From August/2018 to July/2019, mist nets were used to capture birds, and their ectoparasites were then collected. A total of 78 bird species from 18 families were captured, of which 25 species and 12 families were parasitized by lice, while 13 species from 8 families were parasitized by ticks. Of the 546 birds captured, 11.9% were infested by 747 lice belonging to 12 taxa, and 4.2% by 27 ticks. Only seven individuals were infested by both lice and ticks. Both lice and ticks showed preference for topographic sites on their host bodies.

Description of the echolocation pulses of insectivorous bats with new records for Southwest Colombia

Despite the high diversity of bats in neotropics, traditional methods such as mist nets, harp traps and roost detection have limitations in capturing that diversity in a landscape, with most detected species restricted to those that forage in the undergrowth or enclosed spaces. Therefore, acoustic records become a tool that complements and enhances the efforts to get more complete bat inventories while avoiding alterations in usual foraging activities and disruption in their life cycles. This study describes the acoustic parameters (spectral and temporal variables) of the echolocation pulses of insectivorous bats to characterise different species of bats in Southwest Colombia acoustically. We recorded echolocation calls between December 2017 and May 2020 in the Andean and Pacific regions of the Department of Nariño. We analysed 81 sequences of echolocation calls from eight bat species belonging to three families: Vespertilionidae, Molossidae and Emballonuridae. We perform recordings on free-flying bats with identity corroboration by capture for recording in flight rooms and examination in the hand. Myotis riparius and Lasiurus blossevillii were recorded for the first time in the Nariño Department. M. albescens, M. keaysi, M. riparius and L. blossevillii (Vespertilionidae) had pulses of frequency modulated (FM) with a quasi-constant frequency (QCF) ending; Molossus molossus, Tadarida brasiliensis and Promops centralis (Molossidae) had pulses with constant frequency (CF) and QCF; and Saccopteryx bilineata (Emballonuridae) had pulses with QCF. This study contributes to the efforts to facilitate the identification of insectivorous bats of the Neotropics using the acoustic monitoring approaches, represents a reference to compare the acoustic studies in Southwestern Colombia and contributes to increasing our knowledge of the bat diversity in the region.

The role of feeding roosts in seed dispersal service bats provide in urban areas

AbstractIn urban ecosystems, bats play an essential role in seed dispersal, which is facilitated by their use of feeding roosts. However, there are several knowledge gaps in this ecosystem service that bats provide in urban areas. We aimed to describe the fruits consumed by bats in an urban area, the selection and use of feeding roosts, and the patterns of seed dispersal by fruit bats in a Brazilian city. We collected and identified the seeds deposited under feeding roosts and tested for differences in the size of the seed of fruits consumed by bats and the seeds of fruits available in the study area. We also captured and identified bats feeding near roosts using mist nets and assessed the vegetation structure and urban variables that might influence the bats' tree choice for roosts. We found that Artibeus lituratus, Artibeus planirostris, and Platyrrhinus lineatus potentially used feeding roosts and preferred the tallest trees, which were located further away from artificial light. The average distance of seed dispersal was 105.5 meters, and the average size of the dispersed seeds did not differ from the seeds of the fruits available in the area, suggesting that bats are not limited to dispersing only tiny seeds. However, the prevalence of exotic seeds raises concerns that bats may spread exotic species, especially in surrounding protected areas. These results highlight the importance of frugivorous bats as significant seed dispersers in urban areas and emphasize the need for further research to understand their role in shaping urban ecosystems.Abstract in Portuguese is available with online material.

Bat-Associated Pathogenic Leptospira spp. from Forest Fragments in Southwestern Brazilian Amazonia

Bats are known as potential carriers of different pathogens; these animals have been identified worldwide as an important reservoir of different species of Leptospira. Therefore, there has been an increasing interest in studying leptospirosis in neotropical bats in the Amazon. This region is a fertile ground for zoonotic diseases, given the intense process of deforestation, urbanization, opening of new agricultural frontiers, predatory hunting, effects of climate change, and loss of biodiversity. Based on this, the aim of the present study was to investigate the frequency of infection associated with the genetic characterization of pathogenic Leptospira spp. in bats inhabiting diverse landscapes in the southwestern region of the Amazon. To conduct the study, mist nets were installed to capture bats. Kidney samples were submitted to LipL32-polymerase chain reaction and secY gene sequencing. Our results showed that 21% of neotropical evaluated bats in Southwestern Amazon were infected with Leptospira spp. Positive animals were found in rural, urban, periurban, and control areas. Sanguinivores had the highest frequency of positives, followed by insectivores and frugivorous bats. The species of L. interrogans and a potential new Leptospira species were identified. The frequency of Leptospira in bats was not influenced by landscape type, suggesting these pathogens may not be affected by landscape changes. The findings suggest that bats may serve as potential reservoirs of Leptospira in diverse landscapes. The presence of Leptospira in bats appears to be independent of the type of land use, implying that these pathogens may not be affected by small-scale changes in the environment.

Global prevalence of Plasmodium infection in wild birds: A systematic review and meta-analysis

Avian malaria is a vector-borne parasitic disease caused by Plasmodium infection transmitted to birds by mosquitoes. The aim of this systematic review was to analyze the global prevalence of malaria and risk factors associated with infection in wild birds. A systematic search of the databases CNKI, WanFang, VIP, PubMed, and ScienceDirect was performed from database inception to 24 February 2023. The search identified 3181 retrieved articles, of which 52 articles met predetermined inclusion criteria. Meta-analysis was performed using the random-effects model. The estimated pooled global prevalence of Plasmodium infection in wild birds was 16%.Sub-group analysis showed that the highest prevalence was associated with adult birds, migrant birds, North America, tropical rainforest climate, birds captured by mist nets, detection of infection by microscopy, medium quality studies, and studies published after 2016. Our study highlights the need for more understanding of Plasmodium prevalence in wild birds and identifying risk factors associated with infection to inform future infection control measures.

Use of Copernicia vespertilionum as Habitat for Mormopterus minutus (Chiroptera: Molossidae) in Ciego De Avila, Cuba

Mormopterus minutus is a bat endemic to Cuba and its preferred habitat is the palm Copernicia vespertiliorum. Due to its specialization and the loss of its habitats due to anthropogenic action, the species is classified as Vulnerable. The aim of this work is to characterize the population of C. vespertilionum as possible refuges of M. minutus in the Loma de Santa Maria Wildlife Refuge protected area. Between March and August 2019, 4 random grids were marked in the Coastal Savannah for sampling the palm population. Night time counts of the bats were carried out using camera traps and basic morphometry was performed on individuals captured with mist nets. There were 27 specimens of C. vespertilionum with five active colonies that had an average height of 9.81m, while the refuge areas showed an average height of 2.75m. Colonies can present between 2000 and 5500 individuals per refuge. The alar expansion showed an average of 21.8cm, the length of the forearm an average of 9.65cm and the weight of the species averaged 6.5 g. It was determined that forest fires, as a result of anthropogenic actions, were the main cause of the decrease in the population of M. minutus in the protected area. Finally, measures and actions were established for the conservation and protection of the palm trees and with it, of the bat.

Preliminary Study of Chiroptera at Padang Chong Forest Reserve, Hulu Perak, Peninsular Malaysia

The Padang Chong Forest Reserve (PCFR) is a significant part of the Central Forest Spine (CFS) initiative, specifically A-PL2. However, the information regarding chiropterans in PCFR is not extensively documented. To address this gap, our study aims to comprehensively document the occurrence of bats at PCFR. Surveys on bats were conducted in PCFR for five sampling sessions between June and November 2022. Two study plots were established at a distance of 500 m (Plot 1, P1) and 1000 m (Plot 2, P2) from the forest edge. A total of 10 mist nets and three harp traps were deployed in each study plot to capture the bats. Overall, 102 individuals and 24 species from four families of bats were recorded in PCFR. The Papillose Woolly Bat (Kerivoula papillosa) was the most abundant species recorded in this study, followed by the Forest Short- nosed Fruit Bat (Cynopterus cf. brachyotis ‘Forest’), with 15 and 13 individuals, respectively. P1 recorded more bat species than P2, with 19 and 16 species, respectively. The detection of bats in the forest reserve might be influenced by the availability of roosting sites, food resources, and foraging areas. These findings provide a strong basis for developing conservation strategies, and management plans to protect bat habitats in Perak.

Surveys of Avifauna at Gunung Inas Forest Reserve in Kedah, Malaysia

Surveys of avifauna were conducted at Gunung Inas Forest Reserve in Kedah, which constitutes under the Central Forest Spine (CFS) ecological corridor named K-PL1: Ulu Muda Forest Reserve – Gunung Inas Forest Reserve. The surveys were carried out in September and October 2023. The objective of the study is to update the checklist of avifauna recorded at the Gunung Inas Forest Reserve. In this study, two study plots of one-hectare each were established and referred to as Gunung Inas Forest Reserve I (Gunung Inas FR I) and Gunung Inas Forest Reserve II (Gunung Inas FR II). The one-hectare size plot consists of 10 transect lines. A total of 10 mist nets were deployed at each site. The mist-nets were set for five consecutive nights per session, with two sampling sessions per site. In additions, direct observations were also conducted especially for nocturnal and canopy-dwelling species. A total of 47 individuals, comprising 31 species of avifauna (18 families) were documented. In this study, Gunung Inas FR I obtained a higher number of recorded avifauna species with a total of 22 species (15 families), whilst Gunung Inas FR II harbor least records with 16 species (12 families). There is an additional of 28 species recorded during these surveys in comparison to the previous studies, thus, giving the overall cumulative avifauna species recorded is 84 species. Through these results, it is shown that this fragmented forest under K-PL1 still resides by a vast array of avifauna species. Hence, it is hoped that the significance of Gunung Inas FR as a vital habitat for these flying vertebrates can be further emphasized, and appropriate conservation measures must be taken to safeguard their population.

Study of bats in the upper Neretva River valley (Bosnia and Herzegovina)

The upper Neretva River valley with river and tributaries, preserved forests, natural caves and buildings represents a very interesting area for bats. During two field expeditions in summer 2022, we collected data on bats using a variety of methods: inspecting the potential roosts, mist netting, and recording bat echolocation calls. We used manual and automatic ultrasound detectors most frequently at sites along and near the Neretva River. The morphological identification of some species was confirmed using DNA analyses. We detected at least 13 bat species in the study area extending from Krupac to Konjic. With one addition, known from the literature, 14 different bat species have been found in the upper Neretva River valley, presenting nearly a half of all bat species recorded in Bosnia and Herzegovina. We confirmed the presence of three species of the highest conservation concern in Europe, listed in the Annex II of the Habitats directive: lesser horseshoe bat (Rhinolophus hipposideros), greater horseshoe bat (Rhinolophus ferrumequinum), and greater mouse-eared bat (Myotis myotis). Our finding of the alcathoe bat (Myotis alcathoe) presents new confirmation of this species in Bosnia and Herzegovina. Also, observation of the parti-coloured bat (Vespertilio murinus) is one of the few records of the species in this country. The upper Neretva River valley can be considered as an area of high conservation importance for bats. Additional fieldwork, including during other seasons, would likely reveal more bat species and their sites in the area of the upper Neretva River valley.

Factors that interfere with the estimation of bat richness in Atlantic Forest, southeastern Brazil

Capture success of bats can be affected not only by local bat density, but also by several methodological aspects. Many factors interfere with the sampling completeness and capture success of bats. Considering the variety of sampling methods used for investigating, the objective of the present study is to analyze different factors that interfere with sampling completeness and species richness estimates of bats. To carry out the present study, we used data from the State of Rio de Janeiro from inventories carried out from 1989 to 2013 by the Laboratory of Bat Diversity and data from the literature until 2023. We analyzed the following parameters: number of sampling nights, period of nocturnal sampling, number of sites where the mist nets were opened, sampling in diurnal roosts, and moon phase. We observed that the localities with more than 30 species recorded had huge sampling efforts. For studies based on a small capture effort, the active search for roosts during the day increases the probability of recording species found mainly in high sampling effort studies. The most complete inventories are those with a sampling effort of at least 24 nights, including sampling in different habitats, such as trails, water bodies, and close to fruiting plants, as well those including search for day roosts and with fieldwork not restricted to a specific moon phase. Mist nets should be set up not only on trails and close to fruiting trees, but also over water bodies to increase the probability of recording insectivorous species.

Keragaman Jenis Mamalia di Kawasan Ekowisata Mangrove Cukunyinyi Kabupaten Pesawaran

Mangrove ecosystems are important coastal ecosystems that provide a variety of functions. Mangrove ecosystems provide a variety of environmental services including absorbing carbon and contributing to climate change mitigation, protecting coastal areas from storms, tsunamis, and erosion. In addition to providing a variety of environmental services, these ecosystems are also habitats for a variety of living organisms, both aquatic and terrestrial, resident and migrant, also nationally and internationally protected species. The benefits provided by mangrove are contrast to the rate of degradation, which has the potential for loss of wildlife habitat and a decrease in biodiversity value, especially mammals. This study was conducted to analyse the value of biodiversity, especially mammals in the Mangrove Ecotourism Area, Pesawaran Regency. This study uses the method of line transects, mist nets, and sound identification. The results of the study recorded 6 species of mammals with a species diversity value (H') of 1.46, richness of 1.60, evenness of 0.72, and species dominance of 0.30. Mammal species diversity inside the ecotourism area is higher than outside the area. Protected mammals are not found in the area.

Prevalence of West Nile virus antibodies in wild birds in Bangladesh

ObjectivesWest Nile virus is a zoonotic arbovirus spread by mosquito vectors for which birds are common reservoir hosts. In this report, we are presenting the prevalence of West Nile virus antibodies among the resident and migratory wild birds from different locations in Bangladesh. MethodsBirds were captured using mist nets and leg nooses from different areas of Bangladesh. Serum samples were tested using competitive enzyme-linked immunosorbent assay, and swab samples were tested using reverse transcription polymerase chain reaction. ResultsSamples from 888 resident and migratory birds tested, 48 migratory birds (48 of 888, 5.4 %, confidence interval 4–7 %) were found sero-positive for West Nile virus antibody. All the sero-positive birds were from Hakaluki haor and Tanguar hoar, which suggest local transmission of this virus, though we did not find any evidence of active infection. ConclusionsFurther study is required to better understand the role of these birds in the epidemiology of this virus.

Glosophagine bats (Chiroptera: Phyllostomidae) as floral visitors of Stenocereus huastecorum (Plantae: Cactaceae) in the Jaumave Valley, Tamaulipas, Mexico

The objectives of the present work were to determine the species of bats as floral visitors of Stenocereus huastecorum Alvarado-Sizzo, Arreola-Nava & Terrazas, 2018 in the Jaumave Valley, the night activity patterns of these floral visitors, their abundance within the flowering periods of 2018 and the comparison of effectiveness of nocturnal and diurnal pollinators. During March-May and August-October 2018, 12 nights were sampled per flowering period using three mist nets of 12 x 2.6 meters. Bats captured were identified, samples of pollen were taken from them which was processed by Erdtman's acetolysis. Three different treatments were applied to evaluate pollination effectiveness (nocturnal exclusion, diurnal exclusion, and control), selecting three flowers in 19 individuals. Leptonycteris yerbuenae (Martínez & Villa, 1940), L. nivalis (Saussure, 1860) and Choeronycteris mexicana (Tschudi, 1844) were the nocturnal floral visitors of S. huastecorum during its two flowering periods, the first species being the most frequently recorded throughout the night. Although this species has flowers with chiropterophilous characteristics, no significant differences were found in the effectiveness of diurnal, nocturnal and control pollinators. Very little is known about the nocturnal floral visitors of S. huastecorum and the effectiveness of its pollinators in the north of its distribution. Since this species is very important for the economy of rural communities due to the sale of its fruits, this research provides basic information for the conservation and use of this species.