Mississippi Flyway Research Articles

Low-Pathogenic Influenza A Viruses in North American Diving Ducks Contribute to the Emergence of a Novel Highly Pathogenic Influenza A(H7N8) Virus.

Introductions of low-pathogenic avian influenza (LPAI) viruses of subtypes H5 and H7 into poultry from wild birds have the potential to mutate to highly pathogenic avian influenza (HPAI) viruses, but such viruses' origins are often unclear. In January 2016, a novel H7N8 HPAI virus caused an outbreak in turkeys in Indiana, USA. To determine the virus's origin, we sequenced the genomes of 441 wild-bird origin influenza A viruses (IAVs) from North America and subjected them to evolutionary analyses. The results showed that the H7N8 LPAI virus most likely circulated among diving ducks in the Mississippi flyway during autumn 2015 and was subsequently introduced to Indiana turkeys, in which it evolved high pathogenicity. Preceding the outbreak, an isolate with six gene segments (PB2, PB1, PA, HA, NA, and NS) sharing >99% sequence identity with those of H7N8 turkey isolates was recovered from a diving duck sampled in Kentucky, USA. H4N8 IAVs from other diving ducks possessed five H7N8-like gene segments (PB2, PB1, NA, MP, and NS; >98% sequence identity). Our findings suggest that viral gene constellations circulating among diving ducks can contribute to the emergence of IAVs that affect poultry. Therefore, diving ducks may serve an important and understudied role in the maintenance, diversification, and transmission of IAVs in the wild-bird reservoir.IMPORTANCE In January 2016, a novel H7N8 HPAI virus caused a disease outbreak in turkeys in Indiana, USA. To determine the origin of this virus, we sequenced and analyzed 441 wild-bird origin influenza virus strains isolated from wild birds inhabiting North America. We found that the H7N8 LPAI virus most likely circulated among diving ducks in the Mississippi flyway during autumn 2015 and was subsequently introduced to Indiana turkeys, in which it evolved high pathogenicity. Our results suggest that viral gene constellations circulating among diving ducks can contribute to the emergence of IAVs that affect poultry. Therefore, diving ducks may play an important and understudied role in the maintenance, diversification, and transmission of IAVs in the wild-bird reservoir. Our study also highlights the importance of a coordinated, systematic, and collaborative surveillance for IAVs in both poultry and wild-bird populations.

Changes in Distribution of Canada Geese Nesting in Arkansas

The reintroduced Canada goose (Branta canadensis) population in Arkansas has grown in range and abundance in recent decades. We determined the geographic range of Arkansas resident Canada geese from 2004 to 2012 using volume contour maps from citizen science observations using eBird, a citizen science website, and hunter recovery locations from the U.S. Geological Survey Bird Banding Laboratory. Resulting maps indicate an increase in Canada goose encounters toward northwestern and southwestern Arkansas from the original relocations in the Arkansas River valley. We examined movement of Canada geese banded and recovered in Arkansas by determining the distance and angle of movement between initial and final encounter locations; 25% moved east, and 17% went west. The average distance moved from banding to recovery was 50 km (SE = 1 km). Recoveries of Canada geese banded in Arkansas were greatest in the Mississippi Flyway (58% of all geese) followed by the Central Flyway (37%) with some representation in both the Atlantic (4%) and Pacific flyways (0.9%). Movement from Arkansas to other states and Canada was influenced by goose age and sex. Older individuals traveled longer distances than younger ones, and females traveled longer distances than males. Our findings suggest that recently established Canada geese in Arkansas have slowly expanded within the state to the northwest and southwest with the expansion to the east being important now. Movement of Arkansas resident Canada geese on molt-migration can contribute to management issues in other states and provinces.

Projected Influences of Changes in Weather Severity on Autumn-Winter Distributions of Dabbling Ducks in the Mississippi and Atlantic Flyways during the Twenty-First Century.

Projected changes in the relative abundance and timing of autumn-winter migration are assessed for seven dabbling duck species across the Mississippi and Atlantic Flyways for the mid- and late 21st century. Species-specific observed relationships are established between cumulative weather severity in autumn-winter and duck population rate of change. Dynamically downscaled projections of weather severity are developed using a high-resolution regional climate model, interactively coupled to a one-dimensional lake model to represent the Great Lakes and associated lake-effect snowfall. Based on the observed relationships and downscaled climate projections of rising air temperatures and reduced snow cover, delayed autumn-winter migration is expected for all species, with the least delays for the Northern Pintail and the greatest delays for the Mallard. Indeed, the Mallard, the most common and widespread duck in North America, may overwinter in the Great Lakes region by the late 21st century. This highlights the importance of protecting and restoring wetlands across the mid-latitudes of North America, including the Great Lakes Basin, because dabbling ducks are likely to spend more time there, which would impact existing wetlands through increased foraging pressure. Furthermore, inconsistency in the timing and intensity of the traditional autumn-winter migration of dabbling ducks in the Mississippi and Atlantic Flyways could have social and economic consequences to communities to the south, where hunting and birdwatching would be affected.

Using Banding and Encounter Data to Investigate Movements of Red-Tailed Hawks in the Northeastern United States

We examined encounter records from 1925 to 2015 for Red-tailed Hawks banded in the northeastern U.S.A. (n = 1002) and characterized movement patterns by evaluating straight-line distances, difference in latitude, and directionality between banding and encounter locations. We define “encounter” as any handling of a live banded bird, any recovery (a banded bird found dead), and any resighting (reading and reporting a band number on a live bird without actually handling the bird). The mean direction of all hawk encounters was to the southwest of banding locations but some hawks ranged widely; encounters occurred in 27 states, including some in the Mississippi flyway, and in four Canadian provinces. Hawks banded as nestlings or as hatch-year birds (younger) were encountered at farther distances and across a wider range of latitudes than hawks banded as after-hatch-year (AHY) or after-second-year (ASY; older). Most encounters were of younger hawks. In mid-century, most known mortalities were due to shooting; more recent mortalities were due to collisions with vehicles, buildings, or other objects. Some young hawks apparently dispersed out of their natal areas or began migration by three mo post-fledging, and hawks hatched in the Northeast apparently do not migrate north during their first summer. Hawks banded at higher latitudes were encountered at locations farther south than hawks banded at lower latitudes, substantiating leapfrog migration in this species. Encounter data suggested philopatric tendencies; hawks banded as nestlings and encountered at >46 mo of age were encountered significantly closer to their natal areas than hawks banded as nestlings and encountered 46 mo and >250 km from their natal area were considered long-distance dispersers. Between 1930 and 2010, for all hawks banded during the breeding season or summer and encountered in autumn or winter, the difference between banding latitude and encounter latitude decreased by about 4°, suggesting migratory short-stopping.

Identification of migratory bird flyways in North America using community detection on biological networks.

Migratory behavior of waterfowl populations in North America has traditionally been broadly characterized by four north-south flyways, and these flyways have been central to the management of waterfowl populations for more than 80 yr. However, previous flyway characterizations are not easily updated with current bird movement data and fail to provide assessments of the importance of specific geographical regions to the identification of flyways. Here, we developed a network model of migratory movement for four waterfowl species, Mallard (Anas platyrhnchos), Northern Pintail (A. acuta), American Green-winged Teal (A. carolinensis), and Canada Goose (Branta canadensis), in North America, using bird band and recovery data. We then identified migratory flyways using a community detection algorithm and characterized the importance of smaller geographic regions in identifying flyways using a novel metric, the consolidation factor. We identified four main flyways for Mallards, Northern Pintails, and American Green-winged Teal, with the flyway identification in Canada Geese exhibiting higher complexity. For Mallards, flyways were relatively consistent through time. However, consolidation factors revealed that for Mallards and Green-winged Teal, the presumptive Mississippi flyway was potentially a zone of high mixing between other flyways. Our results demonstrate that the network approach provides a robust method for flyway identification that is widely applicable given the relatively minimal data requirements and is easily updated with future movement data to reflect changes in flyway definitions and management goals.

Winter survival of female American Black Ducks in Tennessee, USA

ABSTRACT Many migratory bird species, including the American Black Duck (Anas rubripes; hereafter black duck), face challenges to their survival during winter due to potentially limited resources and high energetic demands. These winter processes can be especially important for the population dynamics of migratory species. Despite stabilization of the overall black duck breeding population, historical declines continue for black ducks wintering in the Mississippi Flyway. It remains unclear if declining abundance in this region reflects high winter mortality. We radio-tagged 111 females in the Tennessee National Wildlife Refuge (TNWR), a major wintering area for mid-continent Mississippi Flyway black ducks, from December to February of 2010–2012 to estimate winter survival and investigate factors that may influence survival rates, including body mass, age, hunting period, and weather. Winter survival (0.83–0.85) was greater than or comparable with previous estimates for black duck populations in North Amer...

Antigenic Characterization of H3 Subtypes of Avian Influenza A Viruses from North America.

Besides humans, H3 subtypes of influenza A viruses (IAVs) can infect various animal hosts, including avian, swine, equine, canine, and sea mammal species. These H3 viruses are both antigenically and genetically diverse. Here, we characterized the antigenic diversity of contemporary H3 avian IAVs recovered from migratory birds in North America. Hemagglutination inhibition (HI) assays were performed on 37 H3 isolates of avian IAVs recovered from 2007 to 2011 using generated reference chicken sera. These isolates were recovered from samples taken in the Atlantic, Mississippi, Central, and Pacific waterfowl migration flyways. Antisera to all the tested H3 isolates cross-reacted with each other and, to a lesser extent, with those to H3 canine and H3 equine IAVs. Antigenic cartography showed that the largest antigenic distance among the 37 avian IAVs is about four units, and each unit corresponds to a 2 log 2 difference in the HI titer. However, none of the tested H3 IAVs cross-reacted with ferret sera derived from contemporary swine and human IAVs. Our results showed that the H3 avian IAVs we tested lacked significant antigenic diversity, and these viruses were antigenically different from those circulating in swine and human populations. This suggests that H3 avian IAVs in North American waterfowl are antigenically relatively stable.

Demographic and Spatiotemporal Patterns of Avian Influenza Infection at the Continental Scale, and in Relation to Annual Life Cycle of a Migratory Host.

Since the spread of highly pathogenic avian influenza (HPAI) H5N1 in the eastern hemisphere, numerous surveillance programs and studies have been undertaken to detect the occurrence, distribution, or spread of avian influenza viruses (AIV) in wild bird populations worldwide. To identify demographic determinants and spatiotemporal patterns of AIV infection in long distance migratory waterfowl in North America, we fitted generalized linear models with binominal distribution to analyze results from 13,574 blue-winged teal (Anas discors, BWTE) sampled in 2007 to 2010 year round during AIV surveillance programs in Canada and the United States. Our analyses revealed that during late summer staging (July-August) and fall migration (September-October), hatch year (HY) birds were more likely to be infected than after hatch year (AHY) birds, however there was no difference between age categories for the remainder of the year (winter, spring migration, and breeding period), likely due to maturing immune systems and newly acquired immunity of HY birds. Probability of infection increased non-linearly with latitude, and was highest in late summer prior to fall migration when densities of birds and the proportion of susceptible HY birds in the population are highest. Birds in the Central and Mississippi flyways were more likely to be infected compared to those in the Atlantic flyway. Seasonal cycles and spatial variation of AIV infection were largely driven by the dynamics of AIV infection in HY birds, which had more prominent cycles and spatial variation in infection compared to AHY birds. Our results demonstrate demographic as well as seasonal, latitudinal and flyway trends across Canada and the US, while illustrating the importance of migratory host life cycle and age in driving cyclical patterns of prevalence.

Census of the Delta State University woody plant community and its implications for ecosystem health

Native plant communities support a diverse biota of invertebrates, birds, and other wildlife, and are therefore essential to the health of ecosystems. Agriculture and urban development have eliminated most native plant communities through forest clearing and introduction of exotic, invasive plants. Prior to European settlement, bottomland hardwood forests prevailed along broad floodplains within the lower Mississippi alluvial valley. Dominant taxa included Taxodium distichum (L.) Rich. (bald cypress), Quercus spp. (oaks), Carya spp. (hickories), Fraxinus pennsylvanica (green ash), Liquidambar styraciflua (sweetgum), Celtis laevigata (sugarberry), Ulmus spp. (elms), and Nyssa spp. (gums). Much of this forested area, however, has been cleared and used for agricultural purposes or urban development. Bottomland hardwood forests, situated along the Mississippi River, are associated with the Mississippi Flyway, a bird migration route heavily utilized by migratory waterfowl, songbirds, and shorebirds for food, water, and shelter. In this preliminary study, woody plant species (trees and shrubs) occurring on the 134 hectare Delta State University campus (33°44.756′N, 90°44.026′W) were identified and documented. At present 46 families, 77 genera, and 125 species have been recognized. Only 66 species are native to the state of Mississippi. Only 41 species are native to the Mississippi Delta. We encourage the planting of more native woody plants representative of bottomland hardwood forests that will benefit migratory wildlife and enhance native habitat and food availability.

H7N9 influenza A virus in turkeys in Minnesota.

Introductions of H7 influenza A virus (IAV) from wild birds into poultry have been documented worldwide, resulting in varying degrees of morbidity and mortality. H7 IAV infection in domestic poultry has served as a source of human infection and disease. We report the detection of H7N9 subtype IAVs in Minnesota (MN) turkey farms during 2009 and 2011. The full genome was sequenced from eight isolates as well as the haemagglutinin (HA) and neuraminidase (NA) gene segments of H7 and N9 virus subtypes for 108 isolates from North American wild birds between 1986 and 2012. Through maximum-likelihood and coalescent phylogenetic analyses, we identified the recent H7 and N9 IAV ancestors of the turkey-origin H7N9 IAVs, estimated the time and geographical origin of the ancestral viruses, and determined the relatedness between the 2009 and 2011 turkey-origin H7N9 IAVs. Analyses supported that the 2009 and 2011 viruses were distantly related genetically, suggesting that the two outbreaks arose from independent introduction events from wild birds. Our findings further supported that the 2011 MN turkey-origin H7N9 virus was closely related to H7N9 IAVs isolated in poultry in Nebraska during the same year. Although the precise origin of the wild-bird donor of the turkey-origin H7N9 IAVs could not be determined, our findings suggested that, for both the NA and HA gene segments, the MN turkey-origin H7N9 viruses were related to viruses circulating in wild birds between 2006 and 2011 in the Mississippi Flyway.

Lead and eagles: demographic and pathological characteristics of poisoning, and exposure levels associated with other causes of mortality

We conducted a retrospective analysis to evaluate demographic and pathologic characteristics in 484 bald eagles (Haliaeetus leucocephalus) and 68 golden eagles (Aquila chrysaetos) diagnosed with lead poisoning at the U.S. Geological Survey National Wildlife Health Center. As part of our analysis, we compared characteristics of lead poisoned eagles with those that died of other causes. Odds of lead poisoning were greater for bald eagles versus golden eagles, females versus males, adults versus juveniles, and eagles from the Mississippi and Central flyways versus the Atlantic and Pacific flyways. In addition to spatial, species, and demographic associations, we detected a distinct temporal trend in the collection date of lead poisoned bald eagle carcasses. These carcasses were found at greater frequency in late autumn and winter than spring and summer. Lesions in lead poisoned birds included emaciation, evidence of bile stasis, myocardial degeneration and necrosis, and renal tubular nephrosis and necrosis. Ingested lead ammunition or fragments were found in 14.2% of bald eagles and 11.8% of golden eagles. The overall mean liver lead concentration (wet weight basis) for eagles diagnosed with lead poisoning was 28.9 ± 0.69 SE mg/kg in bald eagles and 19.4 ± 1.84 SE mg/kg in golden eagles. In eagles diagnosed with collision trauma, electrocution, poisoning (other than lead), emaciation, infectious disease, trapping death, other, and undetermined causes, average liver lead concentrations were low (<1 mg/kg) and did not differ among causes of mortality. Thus, based on our data, we found no evidence that lead exposure of eagles predisposed them to other causes of mortality.

Exploring fall migratory patterns of American black ducks using eight decades of band-recovery data

As regions of habitat used by migratory waterfowl are subjected to rising anthropogenic pressures, establishing patterns of landscape use during migratory cycles is becoming increasingly important for managing and maintaining populations. Although data collection strategies such as global positioning system (GPS) telemetry promise high-resolution insight on geographic use of contemporary populations, decades of available band recovery records on many species can provide a low-cost, multi-generational alternative for defining broad, historically informed patterns of landscape use. We used nearly a century of band-recovery data to reconstruct patterns of fall migratory landscape use for American black ducks in the Mississippi and Atlantic flyways. We partitioned band recovery positions by month from September to February and spatially analyzed positions using kernel density estimates (KDEs) to delineate geographic regions used by American black ducks and track changes in landscape use during migration. Additionally, we considered the appropriateness of current management strategies, which treat American black ducks as a single population, by testing for differences in month-specific landscape use between ducks banded in the Mississippi and Atlantic flyways, and between international management units (ducks banded in Canada or the U.S.). We found that geographic distributions during peak migration faithfully recovered regions previously hypothesized as migratory corridors. Furthermore, regardless of banding origin, we found highly similar distributions and strong flyway fidelity in Atlantic flyway American black ducks. Conversely, American black ducks banded in the Mississippi flyway displayed differences in landscape use between Canadian and U.S. populations, with Canadian ducks significantly more likely to winter in areas within the Atlantic flyway (i.e., migrate between flyways). Flyway- and population-specific patterns of black duck landscape use indicate populations behave more independently than currently treated by management models (including the Adaptive Harvest Management plan), and that population maintenance may be advanced by managing stocks separately. © 2014 The Wildlife Society.

Density and abundance of Wilson's snipeGallinago delicatain winter in the Lower Mississippi Flyway, USA

Wilson's snipeGallinago delicatais one of the least studied North American game birds, and information on snipe populations and abundance is mostly unknown. We conducted roadside surveys stratified at the township level in the lower Mississippi Alluvial Valley (LMAV) in Arkansas, Mississippi and Louisiana, as well as the Red River Region, and the Gulf Coastal Plain of Louisiana during winters of 2009 and 2010. We identified observer, vegetation cover, and water cover as important covariates in estimating snipe densities. We detected 2915 snipe along 814 line transects (1450 km) for 2009 and 2010 combined. We estimated snipe densities of 8.05 individuals km‐2(95% CI: 4.57–14.17) in 2009, and 2.13 individuals km‐2(95% CI: 1.47–3.08) in 2010. We used the resulting snipe density estimates within the study area to calculate abundance estimates of 1 026 431 (95%CI: 582 707‐1 806 774) in 2009, and 271 590 (95%CI: 187 435‐392 722) in 2010 for the LMAV. Our data indicate that a road transect survey method is effective for estimating wintering snipe density and abundance in the lower Mississippi Flyway.

Avian Influenza Virus Prevalence in Migratory Waterfowl in the United States, 2007–2009

We analyzed 155,535 samples collected for surveillance of avian influenza viruses (AIVs), in the United States from 2007 to 2009, from migratory waterfowl (ducks, geese, and swans). The goal was to elucidate patterns of prevalence by flyway and functional groups to determine targets for future surveillance. Apparent prevalence of AIV was highest in the Pacific Flyway in 2007-2008 (14.2% and 14.1%, respectively), in the Mississippi Flyway in 2009 (16.8%), and lowest each year in the Atlantic Flyway (range, 7.3%-8.9%). Dabbling ducks had higher apparent prevalence of AIV (12.8%-18.8%) than diving ducks (3.9%-6.0%) or geese and swans (3.6%-3.9%). We observed highest apparent prevalence in hatch-year waterfowl (15.6%-18.9%). We further analyzed 117,738 of the 155,535 samples to test the hypothesis mallard (Anasplatyrhynchos) had highest prevalence of AIV. We compared apparent prevalence and odds ratios for seven species of ducks and one species of goose commonly collected across the United States. Mallards had highest apparent prevalence (15%-26%) in half of comparisons made, whereas American green- winged teal (Anas creeca, 12%-13%), blue-winged teal (Anas discors, 13%-23%), northern pintail (Anas acuta, 16%-22%), or northern shoveler (Anas clypeata, 15%) had higher apparent prevalence in the remaining comparisons. The results of our research can be used to tailor future surveillance that targets flyways, functional groups, and species with the highest probability of detecting AIV.

Complete Genome Sequence of a Reassortant H14N2 Avian Influenza Virus from California

We report the complete genome sequence of a reassortant H14N2 avian influenza virus isolated in 2011 from a northern shoveler in California. This introduced Eurasian subtype acquired seven segments from North American viruses and circulated in the Pacific Flyway 1 year after its detection in the Mississippi Flyway.

Band reporting probabilities for mallards recovered in the United States and Canada

Reliable estimates of annual harvest rates are required for the implementation of mallard (Anas platyrhynchos) adaptive harvest management decision frameworks. Because not all standard bands recovered during the hunting season are reported, band reporting probabilities are needed to estimate mallard harvest rates. Information from birds recovered with bands that notify finders of a reward (i.e., reward bands) can be used to estimate band reporting rates. We analyzed reward banding data for 3 stocks of mallards to estimate reporting probabilities that can be used to estimate harvest rates for birds recovered with toll-free or web-address bands. Specifically, we explored spatial variability in reporting probabilities, and assessed whether reporting probabilities varied among years. Our analysis indicated that reporting probabilities varied among the 4 Flyways, eastern Canada, and western Canada and Alaska. We had difficulty interpreting temporal fluctuations and found little evidence for any meaningful trends in reporting rates between 2002 and 2010. We recommend that reporting probabilities of 0.67 in the Atlantic Flyway, 0.81 in the Mississippi Flyway, 0.70 in the Central Flyway, 0.76 in the Pacific Flyway, 0.50 in eastern Canada, and 0.57 in western Canada and Alaska be used to estimate harvest probabilities for birds recovered in these regions. © 2013 The Wildlife Society.

Polybrominated diphenyl ethers in birds from Chongming Island, Yangtze estuary, China: Insight into migratory behavior

Sum-PBDEs concentrations in shorebirds and Anatidae ducks muscles from Chongming Dongtan National Nature Reserve ranged from 21–324 to 14–159ngg−1 lw, respectively. PBDEs were detected in muscles of all the studied species. Compared with flyways around the world, migratory waterbirds on the East Asian–Australasian flyway exhibited lower PBDEs burdens than those reported on Black Sea-Mediterranean flyway in Europe and Pacific, Atlantic, Mississippi flyway in North America. Residential Eurasian tree sparrow samples indicated few PBDE products were used in Chongming Island developed in the idea of world famous eco-island. There was no significant difference in PBDEs concentrations between shorebirds and ducks. However, PBDEs composition varied between them. BDE 209 (29–44%) contributed to sum-PBDEs more than BDE 47 (17–19%) in muscles of ducks, while BDE 47 was the predominant congener in shorebirds contributing 32–48%. Stable nitrogen and carbon isotopes and stomach content analysis indicated shorebirds and ducks had the same dietary composition, but showed different preference to bivalves, gastropods and crustaceans for shorebirds and aquatic plant material for ducks. Migratory species had inherent migratory routes and thus had exposure to PBDEs during their stay in breeding grounds, stopover sites and wintering grounds with high use of different commercial PBDE mixtures. Higher percentage of BDE 209 in ducks than shorebirds suggested that breeding ranges and wintering grounds of ducks comprise wetlands in inland and coastal China and Korea where decaBDEs pollution was serious in Asian-Pacific region. Our findings reveal the influence of migratory behavior on PBDEs distribution in migratory waterbirds.

Autumn Migration of Mississippi Flyway Mallards as Determined by Satellite Telemetry

Abstract We used satellite telemetry to study autumn migration timing, routes, stopover duration, and final destinations of mallards Anas platyrhynchos captured the previous spring in Arkansas from 2004 to 2007. Of those mallards that still had functioning transmitters on September 15 (n = 55), the average date when autumn migration began was October 23 (SE = 2.62 d; range = September 17–December 7). For those mallards that stopped for &amp;gt;1 d during migration, the average stopover length was 15.4 d (SE = 1.47 d). Ten mallards migrated nonstop to wintering sites. The eastern Dakotas were a heavily utilized stopover area. The total distance migrated per mallard averaged 1,407 km (SE = 89.55 km; range = 142–2,947 km). The average time spent on migration per individual between September 15 and December 15 was 27 d (SE = 2.88 d; range = 2–84 d). The state where most mallards were located on December 15 was Missouri (11) followed by Arkansas (8), while 5 mallards were still in Canada, and only 8 of 43 females and 0 of 10 males were present in Arkansas. The eastern Dakotas are a heavily utilized migration stopover for midcontinent mallards that may require more attention for migration habitat management. The reasons for so few mallards, especially male mallards, returning to Arkansas the following year deserves further research.

Land Use and Wetland Spatial Position Jointly Determine Amphibian Parasite Communities

Land use change is one of the most commonly cited contributing factors to infectious disease emergence, yet the mechanisms responsible for such changes and the spatial scales at which they operate are rarely identified. The distributions of parasites with complex life cycles depend on interactions between multiple host species, suggesting the net effects of land use on infection patterns may be difficult to predict a priori. Here, we used an information-theoretic approach to evaluate the importance of land use and spatial scale (local, watershed, and regional) in determining the presence and abundance of multi-host trematodes of amphibians. Among 40 wetlands and 160 hosts sampled, trematode abundance, species richness, and the presence and abundance of pathogenic species were strongly influenced by variables at the watershed and regional scales. Based on model averaging results, overall parasite richness and abundance were higher in forested wetlands than in agricultural areas; however, this pattern was influenced by a wetland's proximity to the Mississippi Flyway at the regional scale. These patterns likely reflect the activity of trematode definitive hosts, such as mammals and especially birds, such that infections decreased with increasing distance from the Mississippi River. Interestingly, despite lower mean infections, agricultural wetlands had higher variances and maximum infections. At the wetland scale, phosphorus concentrations and the abundances of intermediate hosts, such as snails and larval amphibians, positively affected parasite distributions. Taken together, these results contribute to our understanding of how altered landscapes affect parasite communities and inform further research on the environmental drivers of amphibian parasite infections.

Determinants of local and migratory movements of Great Lakes double-crested cormorants

AbstractWe investigated how individual strategies combine with demographic and ecological factors to determine local and migratory movements in the double-crested cormorants (Phalacrocorax auritus). One hundred and forty-five cormorants were captured from 14 nesting colonies across the Great Lakes area and fitted with satellite transmitters. We first tested the hypotheses that sexual segregation, density-dependent effects, and the intensity of management operations influenced home range size during the breeding season. The influence of these factors appeared to be limited in part due to random variability in foraging and dispersal decisions at individual and colony levels. We also designed a statistical framework to investigate the degree and determinants of migratory connectivity. Our analyses revealed a significant migratory connectivity in cormorants, although we also observed a nonnegligible amount of individual variability and flexibility. Our data were most consistent with the existence of a migratory divide across the Great Lakes, with western populations using mainly the Mississippi Flyway and eastern populations the Atlantic Flyway. Previous and current studies suggest that the divide cannot be explained by past divergence in isolation, a way to diminish travel cost, or the Appalachians constituting an ecological barrier per se but is rather the consequence of the distribution of suitable stopover and nonbreeding areas. However, a parallel migration system and no migratory divide could not be entirely ruled out with present data.