To explore the effect of interchange spacing on drivers' visual characteristics in the merging areas of interchange, a high-density group of five interchanges on the expressway of Chongqing, China, was selected as the test site. An naturalistic driving test was conducted with 47 participants, and the Tobii Glasses II portable eye tracker was used to collect gaze data during driving. The drivers' fixation field was divided into six areas by applying a K-means dynamic clustering algorithm combined with the actual scenario. Markov chains were used to calculate the drivers' gaze transition probability matrices under different driving conditions, and the analysis of gaze transition behaviors was directed at common spacing interchanges, small spacing interchanges, and composite interchanges. Under the ramp-mainline condition, drivers’ fixations were primarily concentrated on the near ahead and the left side areas, with higher rates of repeated fixations on the left rearview mirror and left-side line areas. The average value of fixation duration, saccade distance, and saccade speed of small spacing interchange is higher than common spacing interchange. Additionally, under the mainline condition, the probability of one-step transition and repeated fixation rates significantly increased for the right-side lane areas, and the average values of fixation index and saccade index of small spacing interchange are lower than those of common spacing interchange. The results show that the highest probabilities of repeated fixation by drivers occurred in the near ahead and far ahead areas in the interchange merging areas. Insufficient spacing resulted in more frequent occurrences of zero values in one-step transition probability matrices. The research conclusions provide theoretical support for the optimal design and safe operation of the merging area of high-density interchange group of urban expressway.