Mint Extract Research Articles

Evaluation of preserving the viability of sheep's teeth PDL cells according to the different times and storage medias

Introdution: Vitality of periodontal ligament (PDL) cells is very critical for replantation of complete avulsion teeth due to traumatic injuries. This is important for transferring an avulsed tooth to clinic for replantation that which Medias used for storage.This study aimed to compare the vitality of PDL cells of sheep teeth cultured in different storage medias including α-Minimum Essential Medium (αMEM), Dulbecco’s Modified Eagle’s Medium (DMEM), Hank’s Balanced Salt Solution (HBSS), and mint extract
 Methods: In this lab trial study, PDL cells were obtained from 124 healthy anterior and posterior sheep teeth and cultured in αMEM, DMEM, HBSS, and mint extract for periods of 2, 6, 24, 48, 72, or 96 hours (24 groups). For each solution, positive control group were PDL cells without incubation in any storage media. For each group, there was a negative control considered cells growing in dry plate with no medium. After exposure of PDL cells to scheduled solution for scheduled incubation time, centrifuge was performed for 10 minutes at rate of 2000G. Then cell percipitates were added into the solution of collagenase (3mgr/ml) and Dispase (4mgr/ml to cell precipitates, which were incubated at 37° C for 60 minutes. After washing cellular suspension in PBS, vitality of the cells was assessed by Trypan blue exclusion, on a neobar slide by magnification of 200X. The data were analyzed statistically using 2-way ANOVA test by SPSS version 15.
 Results: Statistically significant differences in efficacy of different medias were obtained at least between two media (P=0.0001). PDL cells cultured in αMEM and mint extract showed 90% and 52.22% vitality representing, respectively, the best and the weakest storage media.
 Conclusion: αMEM can be a suitable transport medium up to 96 hours to preserve the vitality of the PDL cells of avulsed teeth. There is a reverse correlation between the viability of PDL cells and incubation time, increasing the time decreases the viability.

In Vitro Antioxidant and Antimicrobial Properties of Flower, Leaf, and Stem Extracts of Korean Mint.

Traditionally, Agastache rugosa (Korean mint) has been widely used to treat various infectious diseases. The aims of this study were to: (i) determine the phenylpropanoid content of the plant using high-performance liquid chromatography; (ii) undertake total anthocyanin, flavonoid, and phenolic assays; (iii) and evaluate the antioxidant and antibacterial properties of the methanol extracts from the stem, leaves, and flowers of Korean mint. The total anthocyanin, flavonoid, and phenolic content assays showed that the flowers had higher phenolic levels than the stem and leaves. The reducing power, the 2,2-diphenyl-1-picrylhydrazyl superoxide radical scavenging abilities, and the hydrogen peroxide radical scavenging activities were also evaluated so that the antioxidant activities of the extracts from the different plant parts could be evaluated. The flower extracts revealed higher antioxidant properties than the other parts. The antibacterial properties of the methanol extracts from A. rugosa were analyzed by the disc diffusion method, and the flower extracts had higher antibacterial activities against the six bacterial strains used in the study than the other parts. This study provides information on the synergistic antioxidant and antibacterial properties of phenolics derived from the different parts of Korean mint.

Langmuir, Freundlich, Temkin and Dubinin–Radushkevich isotherms studies removal of silver from aqueous solution by the Mint extract

Langmuir, Freundlich, Temkin and Dubinin–Radushkevich isotherms studies removal of silver from aqueous solution by the Mint extract

Response of Antioxidant Potential and Essential Oil Components to Irrigation and Fertilization on Three Mint Species (Mentha spp. L.)

Mint (Mentha spp.), one of the most used plants in traditional medicine, is cultivated since antiquity, being mentioned by ancient writings. Besides its use for improving the human health, mint extracts are found in food, cosmetics, detergents, perfumes, biopesticides, etc. Due to its multiple benefits mint consumption has increased continuously in the last years. Besides the interest in mint yield, another interesting trait of this species is the antioxidant activity and the quality of essential oils. According to some research in the field, using water given by irrigation and fertilization leads to an increase of green mass production, but the level of essential oils remains the same. The aims of the present research were to determine the effects of irrigation, fertilization and species over the quality of mint and to identify the best matches of the experimental factors for increasing the antioxidants and essential oils content. Plant material was represented by M. spicata, M. piperita and M. suaveolens. Management followed the growing season of mint plants both in non-irrigated and irrigated conditions, with base fertilization and usage of Lignohumate. The analysis of samples was performed using Photochem where photochemical excitation of free radicals is combined with luminometric detection. The composition of the essential oil was determined by Gas chromatography with flame ionisation detector GC-FID. The research concludes that mint quality increased in conditions of no irrigation. For high phytonutrient content it is recommended to cultivate M. piperita, because it has the highest antioxidants content. M. spicata presented the essential oil with the best content of alcohols, esters and aromatic compounds. M. suaveolens reached the best level of esters, aldehydes and aromatic compounds only in the case of fertilization with Lignohumate.

Optimisation of mint active compound extraction with anti-muscarinic property on intestine tissue

Muscle spasms may occur when muscle is overused and tired, particularly if it is overstretched or if it has been held in the same position for a prolonged period of time. Menthol, an anti-spasmodic, is a natural ingredient found in mint (Mentha piperta). This research was aimed, to determine the efficiency of different solvents for menthol extraction through antimuscarinic effect on frog ileum smooth muscle and to determine the optimal menthol concentration for anti-muscarinic effect. Dried mint leaves were ground into powder and compounds were extracted using methanol, ethanol and acetone, all at 100% v/v, 75% v/v and 50% v/v concentration. These extracts at doses ranged from 0.0–3.0 mg/mL were tested on 3cm frog smooth muscle to observe the anti-muscarinic effect. The extraction of menthol was significantly higher in acetone (3.43cm ± 0.23) compared to methanol (3.23cm ± 0.13) and ethanol (3.22cm ± 0.12), p<0.001. Based on the dose response curve, the minimal effective concentration for acetone to produce maximum relaxation was 1.0 mg/mL which higher when compared to positive control, atropine (3.30cm ± 0). In conclusion, acetone mint extract demonstrated significant anti-muscarinic property in smooth muscle relaxation at 1mg/mL comparable to atropine drug.

Characteristics of Japanese Mint Extracts Obtained by Subcritical-water Treatment

Characteristics of Japanese Mint Extracts Obtained by Subcritical-water Treatment

Biologically Synthesized Silver Nanoparticles for Enhancing Tetracycline Activity Against Staphylococcus aureus and Klebsiella pneumoniae

Abstract Phytochemical content of plant extracts can be used effectively to reduce the metal ions to nanoparticles in one-step green synthesis process. In this study, six plant extracts were used for the synthesis of silver nanoparticles (AgNPs). Biologically synthesized AgNPs was characterized using UV-Vis Spectrophotometer, Field Emission Scanning Electron Microscope (FE-SEM), X-ray diffraction (XRD), Energy Dispersive X-ray spectroscopy (EDX) and Fourier Transform Infrared (FTIR) spectroscopy. The individual and combined effects of AgNPs and tetracycline against S. aureus and K. pneumoniae were assessed. Ginger, onion and sidr extracts supported AgNPs formation while arak, garlic and mint extracts failed to convert the silver ions to AgNPs. The present findings revealed significant differences between the tested plant extracts in supporting AgNPs synthesis. AgNPs synthesized by ginger showed the highest individual and combined activity against tested strains followed by AgNPs prepared by sidr then that synthesized by onion. AgNPs significantly enhanced tetracycline activity (p≤0.05) against S. aureus and K. pneumoniae. The results of this study demonstrated that the combination of tetracycline and biologically synthesized AgNPs presented a useful therapeutically method for the treatment of bacterial infection and counterattacking bacterial resistance.

Evaluation of Antioxidant, Anti-Inflammatory and Cytoprotective Properties of Ethanolic Mint Extracts from Algeria on 7-Ketocholesterol-Treated Murine RAW 264.7 Macrophages.

The present study consisted in evaluating the antioxidant, anti-inflammatory and cytoprotective properties of ethanolic extracts from three mint species (Mentha spicata L. (MS), Mentha pulegium L. (MP) and Mentha rotundifolia (L.) Huds (MR)) with biochemical methods on murine RAW 264.7 macrophages (a transformed macrophage cell line isolated from ascites of BALB/c mice infected by the Abelson leukemia virus). The total phenolic, flavonoid and carotenoid contents were determined with spectrophotometric methods. The antioxidant activities were quantified with the Kit Radicaux Libres (KRLTM), the ferric reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. The MS extract showed the highest total phenolic content, and the highest antioxidant capacity, while the MR extract showed the lowest total phenolic content and the lowest antioxidant capacity. The cytoprotective and anti-inflammatory activities of the extracts were quantified on murine RAW 264.7 macrophages treated with 7-ketocholesterol (7KC; 20 µg/mL: 50 µM) associated or not for 24 h and 48 h with ethanolic mint extracts used at different concentrations (25, 50, 100, 200 and 400 µg/mL). Under treatment with 7KC, an important inhibition of cell growth was revealed with the crystal violet test. This side effect was strongly attenuated in a dose dependent manner with the different ethanolic mint extracts, mainly at 48 h. The most important cytoprotective effect was observed with the MS extract. In addition, the effects of ethanolic mint extracts on cytokine secretion (Interleukin (IL)-6, IL-10, Monocyte Chemoattractant Protein (MCP)-1, Interferon (IFN)-ϒ, Tumor necrosis factor (TNF)-α) were determined at 24 h on lipopolysaccharide (LPS, 0.2 µg/mL)-, 7KC (20 µg/mL)- and (7KC + LPS)-treated RAW 264.7 cells. Complex effects of mint extracts were observed on cytokine secretion. However, comparatively to LPS-treated cells, all the extracts strongly reduce IL-6 secretion and two of them (MP and MR) also decrease MCP-1 and TNF-α secretion. However, no anti-inflammatory effects were observed on 7KC- and (7KC + LPS)-treated cells. Altogether, these data bring new evidences on the potential benefits (especially antioxidant and cytoprotective properties) of Algerian mint on human health.

English

The objective of the present study was to assess the anti-fungal activity of medicinal plant extracts on inhibition of Colletotrichum gloesporioides mycelial growth, sporulation and germination and on anthracnose control in Heliconia psitacorum cv. Golden Torch and Heliconia rostrata. Extracts of garlic, lemon grass, cinnamon, lemon balm, eucalyptus, ginger, mint, bitter melon and black pepper were used. The, mycelial were assessed in vitro by measuring their growth in Petri dishes. Sporulation was assessed by counting spores in a Neubauer chamber. Germination was observed based on germination tube emission. In the in vitro tests, the inflorescences were treated with the plant extracts and inoculated with the pathogen 24 h later. The results showed that all the extracts presented anti-fungal activity, in greater or lesser intensity, compared to the control. The garlic extract resulted in the highest mycelial growth inhibition rate. Regarding sporulation, the bitter melon, ginger, mint, popcorn eucalyptus and bitter melon extracts were more efficacious, interfering in spore formation; while the ginger extract most reduced spore germination inhibiting germination tube emission. For the in vivo tests, it was observed that all the plant extracts tested were efficient in reducing lesion severity in the inflorescence, showing that the use of plant extracts may be a promising alternative for managing the diseases that affect helconia post-harvest. Key words: Alternative control, fungi, Colletotrichum gloesporioides, tropical flowers. &nbsp

Activity of Mentha piperita L. Ethanol Extract against Acetic Acid Bacteria Asaia spp.

Acetic acid bacteria belonging to the genus Asaia spp. are relatively new microbial contaminants in the beverage industry. These bacteria cause organoleptic changes such as increased turbidity, haziness and sour odor. In addition, they are able to form biofilms on the inner parts of production lines, and finally they can cause secondary contamination of final products. For this reason, new methods using effective and safe preservatives are being developed to improve microbial stability of soft beverages. The aim of the research was to investigate the effects of Mentha piperita L. ethanol extract against Asaia spp. biofilm formation. The bacterial adhesion was evaluated by a plate count method and luminometry, as well as fluorescence microscopy. The polyphenolic profile of the mint extract was determined on the basis of high-performance liquid chromatography (HPLC). The obtained microbiological results indicate bacteriostatic effect of mint extract at 10% (v/v) concentration. The plant extract also reduces the number of adhered bacterial cells on polystyrene surface.

Effect of Mentha piperita mint water extracts in inhibiting the growth of some pathogenic fungi

The aim of the study was to study the effect of the mineral extract of Munthapiperita in the growth of fungus Rhizoctoniasolani, Aspergillusniger, Aspergillusterreus , Fusariumoxysporum, Fusariumoxysporumf. sp. (100%) , 25%, 50%, 75% and 100%. The results showed the efficacy of the mint extract of the mint plant in inhibiting the growth of fungi and was the highest inhibition when using 100% concentration. Especially after 3,2.1 days of growth. We did not notice that solaniRhizoctonia was affected by the extract, especially after the incubation period of 7,6,5,4 days.

Perbedaan Mual dan Muntah Ibu Hamil Trimester I yang Diberikan Ekstrak Jahe dan Ekstrak Daun Mint

&lt;p&gt;Study of literature with meta-analysis was obtained 7 out of 10 women around the world to experience nausea and vomiting during pregnancy, with an average incidence of 69.4% (66.5-72.3%, CI 95%). The purpose of research to find out the difference of nausea and vomiting of pregnant women through assessment scale Rhodes after administration of ginger extract and mint leaf extract in Inpatient Health Center Bandar Lampung in 2016. The target of research is the discovery of alternative therapy/food in the community to reduce nausea and vomiting of pregnant women. Type of research is quantitative research, design experimental research. The study population is the entire first-trimester pregnant women who visit health centers Inpatient Bandar Lampung, with 10 (ten) PHC hospitalization. The total sample was 102, the group treated with the extract of ginger were 34 respondents, a group treated extract of mint leaves were 34 respondents, and the group without treatment who received standard procedures health centers (Vitamin B6) were 34 respondents, with the criteria of pregnant women the first trimester without GI disease. The results of the study there were significant differences between the measurement value of nausea and vomiting before and after administration of ginger extract, extract of mint and a control group with p = 0.001 in the extract of ginger, p=0.000 in the extract mint, and p=0.001 in the control group with (standard treatment of health centers with vitamin B6). There were no differences between mean nausea and vomiting in the first trimester pregnant women who received ginger extract and mint with a p=0.548, ginger extract and control groups with p=0.807, mint extract and control groups with p=0,407 ginger and mint can be an alternative therapy to reduce vomiting and nausea of pregnant woman in trimester I. Advanced research about the composition, dosage and side effects of ginger and mint benefit to the problem of gastrointestinal disorders who experience nausea and vomiting.&lt;/p&gt;

Plants of Genus Mentha: From Farm to Food Factory.

Genus Mentha, a member of Lamiaceae family, encompasses a series of species used on an industrial scale and with a well-described and developed culture process. Extracts of this genus are traditionally used as foods and are highly valued due to the presence of significant amounts of antioxidant phenolic compounds. Many essential oil chemotypes show distinct aromatic flavor conferred by different terpene proportions. Mint extracts and their derived essential oils exert notable effects against a broad spectrum of bacteria, fungi or yeasts, tested both in vitro or in various food matrices. Their chemical compositions are well-known, which suggest and even prompt their safe use. In this review, genus Mentha plant cultivation, phytochemical analysis and even antimicrobial activity are carefully described. Also, in consideration of its natural origin, antioxidant and antimicrobial properties, a special emphasis was given to mint-derived products as an interesting alternative to artificial preservatives towards establishing a wide range of applications for shelf-life extension of food ingredients and even foodstuffs. Mentha cultivation techniques markedly influence its phytochemical composition. Both extracts and essential oils display a broad spectrum of activity, closely related to its phytochemical composition. Therefore, industrial implementation of genus Mentha depends on its efficacy, safety and neutral taste.

Study of the effect of some alcoholic plant extracts in the control of adult domestic flies. Musca domestica

In this study, the toxic effect of the plant extracts of Mentha Piperita, Datora innoxia, Olea europea, and Dichlorvos (DDVP) were investigated in the domestic flies of Musca domestica. The domestic flies were treated with plant extracts and diclorophosphate with a fine spray method at 30 ± 2 ° C and 70 ± 5% relative humidity. Results were observed 24 to 48 hours of treatment respectively.&#x0D; The results obtained from the present study showed that the vegetal extracts of Datura and Olives were given the highest corrected mortality rates (75.6 and 74.2) for the extracts of Datura and Olives respectively, and through these arithmetic averages, The insect also did not differ statistically but differed in the rates of killing from the ethanol extract of mint leaves and the Dichlorvos (DDVP) used in the experiment.

Seasonal Changes of Phagocytic Activity Enhanced by an Acoholic Mint Extract in Cultured Rainbow Trout

Abstract: Objective: We hypothesized that leukocyte numbers and their in vitro phagocytic activity in farmed rainbow trout are subject to negative influence of temperatures extreme for fish and that mint extract could alleviate these effects. Materials and Methods: Blood samples from farmed rainbow trout collected during winter and summer were subjected to leukocyte subpopulation counts (%) and carbon particle inclusion test to estimate the in vitro phagocytic activity after 15 and 30 min of incubation at 4 and 22°C, with or without alcoholic mint extract (ln). Results and Discussion: There was a significant increase in lymphocytes (p<0.05) and monocytes (p<0.01) in summer versus winter, while the heterophils were dominant during winter (p<0.01). In vitro spontaneous phagocytosis increased with the seasonal increase in temperature (0.425±0.094, winter and 0.835±0.102, summer, p<0.05) and during the first reading period (0 to 15 min, p<0.01). The mint extract increased phagocytosis for the overall reading period from 2x (winter, 0.281±0.221) up to 5x (summer, 1.032±0.221) (p<0.01). Conclusion: The positive activity of the alcoholic mint extract during phagocytosis, more intense during summer than during spring, proved to be a temperature driven process, plant active principles playing a dual stimulating role for both monocytes and heterophiles. Key words: Rainbow trout, Season, Leukocytes, Phagocytosis, Mint extract.

Chitosan Nanoparticles as a Carrier for &lt;i&gt;Mentha longifolia&lt;/i&gt; Extract:Synthesis, Characterization and Antifungal Activity

A recent study was aimed at determining the antifungal activity of chitosan in both its natural and nanopa rticle forms. The present study reports on the prepa ration and characterization of ch itosan nanop articles and chitosan nanoparticle-incorporated Mentha longifolia . The size of the chitosan nanoparticles was 157 nm, and the mint extraction nanoparticles at concen trat ions of 5%, 10% and 20% were 142, 105 and 63 nm respectively. The surfaces of the chi tosan nanoparticles had a positive charge of approximately 33.2 mV, and the mint extract chitosan nanoparticles at 5%, 10% and 20% had charges of approximately 37.5, 36.9 and 36.1 mV respectively. The chitosan nanoparticles had a maximum encapsulation efficiency of 92% at a mint extract concentration of 20%. Mint extract incorporation into chitosan nanoparticles resulted in increased antifungal effects against mycelium growth of A. niger .

EFFECT OF ADDING EXTRACTS OF MINT AND THYME ON THE GROWTH OF MICROORGANISMS IN WHITE SOFT CHEESE MANUFACTURED IN LABORATORY

This study aimed to detect the effect of adding two plant extracts namely; Spearmint (Mintha spicata L.) and Thyme (Thymus vulgaris) at different concentrations on some microbiological and sensory properties of white soft cheese manufactured in the laboratory. Cow milk was used in processing the cheese. Control cheese manufactured by using the rennet only, while the other cheese treatments were made by adding separately different concentrations (10, 20, and 30 ml/100 grams of curd) of mint and thyme extracts to the curd. The produced cheese samples were studied microbiologically and organoleptic ally during storage at intervals (fresh, 3, 6, 9, and 12 days) at 4 C° in the refrigerator. The results of the microbiological tests revealed, the superiority of cheese samples produced by adding the plant extracts in extending the shelf-life of the cheese compared to control samples. Using higher concentrations (20 and 30) of either mint or thyme extract was found to be the most effective in decreasing the total bacterial count, and in earlier disappearance of coliform and staphylococcus compared to the control cheese. The yeast and mold appeared late during the storage in the treated cheese, while they appeared earlier in the control. The results of the organoleptic properties during cold storage revealed, that, all samples of cheese manufactured in this study got scores which made them acceptable by the consumers. It is also found that, there was no negative affect as a result of adding the two types of extracts on the sensory properties of the manufactured white soft cheese.

Optimization of Preparation Process for a Blended Beverage Developed from Chayote, Sugarcane, and Mint and Coriander Extract

ABSTRACTChayote [Sechium edule (Jacq) Swartz] is an underutilized, nutritious vegetable, which is reported to contain a variety of phytochemicals. A process was designed to increase acceptance of Chayote in a beverage using concentrations of sugarcane juice, salt, and mint and coriander herb extract using response surface methodology. The statistical design provided 20 formulations where the herb extract concentration varied from 0.5% to 2.5%, sugarcane juice concentration from 20% to 50%, and salt concentration from 0% to 1.5%. Sugarcane juice concentration of 35.14%, herb extract of 1%, and salt concentration of 1.5% were the optimized process conditions for preparation of blended chayote juice. Optimized conditions can be scaled up, allowing this vegetable to be utilized for processing.

Effect of Chamomile, Wild Mint and Oregano Herbal Extracts on Quality and Quantity of Eggs, Hatchability, and Some Other Parameters in Laying Japanese Quails

According to various reports of beneficial effects of medicinal plants on the performance of broiler chickens and less extensively studies in laying poultries, this study was conducted to find an appropriate and harmless feed additive to enhance the quality and quantity of poultry eggs. The effect of three herbal extracts on quantity and quality of eggs, blood parameters, hatchability, intestinal bacterial population, and intestinal morphology in laying Japanese quail were investigated. The study was applied with 64, ten-week old laying Japanese quails for 8 weeks. The experiment was a completely randomized design with 4 treatments, 4 replications and 4 birds per replicate (the ratio of male to female 1:3). Experimental treatments involved: Control, with no additive in drinking water; chamomile extract; wild mint extract; and oregano extract. Herbal extracts were added 1 mL/L drinking water. The three treatments showed no significant effect on productivity, egg mass, FCR, egg weight, feed intake and qualitative indices of eggs; however, the herbal extracts specially the chamomile extract reduced the cholesterol of eggs (P<0.05). Herbal extracts showed no significantly effects on the hatchability of fertile eggs. Oregano extract showed the best effect on reduction of the intestinal bacterial population and increase the villus height in ileum (P<0.05). Chamomile extract by reducing the yolk cholesterol can improve the egg market’s popularity, and oregano extract by reducing the number of pathogenic bacteria and improving the villus height in ileum can be considered as a beneficial and low-risk additive for laying poultries.

Green Synthesis, Characterization and Antimicrobial Activity of Ag Nanoparticles Using Mint Extract

In this work, we describe the biological method are used for the production of silver nanoparticles using plant extract. Silver (Ag) nanostructures were successfully prepared by a simple, highly efficient, and low-cost using the hydrothermal method by using the mint extract and evaluate their antimicrobial activity. The resulting nanostructures were characterized by XRD, FESEM, and UV-VIS spectroscopy. The nanoparticles structural properties were studied using X-Ray diffraction (XRD) and showed all the diffraction peaks are indexed to (F.C.C) structure. The crystallite size of Ag NPs was calculated and equal to 25 nm. FE-SEM images of silver showed nanoparticles that assembled in flower-like shape with a diameter of 10 nm-20 nm. The optical absorption explained by UV-Visible spectroscopy, the Ag NPs has sharp absorbance with the highest peak at 400 nm. The optical transmittance of the Ag film deposit time was around 40% at wavelength 400 nm then increases sharply at wavelength 400-900 nm. The energy gap increase to 3.4 eV. The antimicrobial activity was evaluated by agar well disc diffusion method against various microorganisms. The zone of inhibition against (Escherichia coli) was 20 mm, and fungus (Bacillus subtilis) was 25 mm. The use of silver nanoparticles in drug delivery systems might be the future thrust in the field of medicine.