Minor Strand Research Articles

Prosper, consume and be saved

A Sydney-based megachurch with global reach, well-known for its “prosperity gospel” of financial acquisition, has developed an additional strand: a detailed theology of consumption. The affinity between a theology of guilt-free—indeed, obligatory—consumption and late capitalism goes some way towards explaining the attraction this minority strand of Christianity holds for politicians, including those without personal religious commitments, in a secular electorate.

The Complete Mitochondrial Genome of the Stalk-Eyed Bug Chauliops fallax Scott, and the Monophyly of Malcidae (Hemiptera: Heteroptera)

Chauliops fallax Scott, 1874 (Hemiptera: Heteroptera: Malcidae: Chauliopinae) is one of the most destructive insect pests of soybean and rice fields in Asia. Here we sequenced the complete mitochondrial genome of this pest. This genome is 15,739 bp long, with an A+T content of 73.7%, containing 37 typical animal mitochondrial genes and a control region. All genes were arranged in the same order as most of other Heteroptera. A remarkable strand bias was found for all nine protein coding genes (PCGs) encoded by the majority strand were positive AT-skew and negative GC-skew, whereas the reverse were found in the remaining four PCGs encoded by the minority strand and two rRNA genes. The models of secondary structures for the two rRNA genes of sequenced true bugs and Lygaeoidea were predicted. 16S rRNA consisted of six domains (domain III is absent as in other known arthropod mitochondrial genomes) and 45 helices, while three domains and 27 helices for 12S rRNA. The control region consists of five subregions: a microsatellite-like region, a tandem repeats region and other three motifs. The unusual intergenic spacer between tRNA-H and ND4 only found in the species of Lygaeoidea, not in other heteropteran species, may be the synapomorphy of this superfamily. Phylogenetic analyses were carried out based on all the 13 PCGs showed that Chauliopinae was the sister group of Malcinae and the monophyly of Lygaeoidea.

Finding the ‘Neo’ in Neoliberalism

‘Liberalism’ and ‘neoliberalism’ have become important shorthand terms in critical work that seeks to incorporate issues of economics into ideological and epochal analyses. Yet, these terms incorporate theoretical histories and refer to historical contexts so vast that they can seem ambiguous and boundaryless. This ambiguity threatens to reduce the analytical usefulness of the terms liberalism and neoliberalism. In this paper, I map the legacies and meanings of the terms liberalism and neoliberalism and diagram the complexity and specificity of what neoliberalism is today. First, I engage a small set of definitions and uses of these terms to try to make sense of liberalism and neoliberalism as historical-theoretical concepts. Second, I group current academic uses of the term neoliberalism into Foucauldian, Marxist and epochalist camps, explaining the limits of each. Third, drawing on a fourth minor strand of work on neoliberalism that opens a path to better defining and using the term, I present my own definition of neoliberalism that distinguishes between a theoretical mode and an articulation mode. I conclude by proposing that what is new in neoliberalism is what I call corporism, the privileging of the form and position of corporations.

Israel: state management of religion or religious management of the state?

In Israel, the Jewish religion, which is unique among world religions in the primacy it accords to filiation rather than belief as a criterion of belonging, operates as a formal criterion of citizenship, but in substance different ways of being Jewish are expressed in different political forces which in turn struggle for control of the state's religious orientation. This political struggle leads the state to favour ultra-Orthodox observance and criteria of belonging, even though that is a minority strand in the country itself and even more so outside. Religious interests and ideologies have found substantial niches in the legal system, in education, in the army and in the West Bank settlements, by exploiting the state's corporatist character, leading to a type of multiculturalism in which the once-secular centre has been seriously eroded.

Rio+20, the Green Economy and Re-Orienting Sustainable Development

This article looks at the role played by the green economy approach to sustainable development in the run-up to and aftermath of the UNCSD 2012 (the Rio+20 Earth Summit). It examines the inherent tensions that have become increasingly apparent within the sustainable development paradigm and considers the impact of the green economy approach as espoused in the Rio+20 process on sustainability. The positions adopted by major actors (comprising institutions, states and major groups) towards the green economy in the Rio+20 process and its outcome document The Future We Want are discussed. The article reflects on the particular manifestation of the perennial North/South tensions that permeate international environmental law and governance that have been prompted by the green economy debate. It also considers new fractures in the South grouping prompted by the increasing influence calls for a less materialistic, more holistic basis for human/ environment relations. Thus, contrary to the economics-dominated agenda espoused in the Rio+20 process, the Mother Earth Rights/Pachamama approach that is being promoted by a number of South American states emerged (albeit as a minor strand) in the outcome document. The article contemplates both the disappointment of the Rio+20 process and outcome and the seeds for a more creative future that may have been sown in them.

Complete mitochondrial genome of the free-living earwig, Challia fletcheri (Dermaptera: Pygidicranidae) and phylogeny of Polyneoptera.

The insect order Dermaptera, belonging to Polyneoptera, includes ∼2,000 extant species, but no dermapteran mitochondrial genome has been sequenced. We sequenced the complete mitochondrial genome of the free-living earwig, Challia fletcheri, compared its genomic features to other available mitochondrial sequences from polyneopterous insects. In addition, the Dermaptera, together with the other known polyneopteran mitochondrial genome sequences (protein coding, ribosomal RNA, and transfer RNA genes), were employed to understand the phylogeny of Polyneoptera, one of the least resolved insect phylogenies, with emphasis on the placement of Dermaptera. The complete mitochondrial genome of C. fletcheri presents the following several unusual features: the longest size in insects is 20,456 bp; it harbors the largest tandem repeat units (TRU) among insects; it displays T- and G-skewness on the major strand and A- and C-skewness on the minor strand, which is a reversal of the general pattern found in most insect mitochondrial genomes, and it possesses a unique gene arrangement characterized by a series of gene translocations and/or inversions. The reversal pattern of skewness is explained in terms of inversion of replication origin. All phylogenetic analyses consistently placed Dermaptera as the sister to Plecoptera, leaving them as the most basal lineage of Polyneoptera or sister to Ephemeroptera, and placed Odonata consistently as the most basal lineage of the Pterygota.

Introduction Locating an Australia‐wide Anthropology

ABSTRACT Ethnographic research concerning Aboriginal social life in the earlier settled areas of the continent has formed a minor strand within the body of Australian anthropological research. Yet these studies speak directly to the current national discourse concerning distressing conditions in many Aboriginal communities in the north. The kind of anthropology generated in South East Australia has always sought to do more than simply depict particular collective worlds—it also addresses how they are made, particularly in conditions of upheaval, relocation and engagement with state institutions and the nation's ideologies. By drawing attention to one kind of border, that between cultural/social worlds we hope to overcome another boundary, that between ethnographies of the north and the south. To this end, we draw attention to the process of boundary making that has been a crucial dynamic within the changing historical and social configurations that have shaped Aboriginal experiences of the social world. Thus, instead of reporting on the significance of cultural characteristics, this collection shifts attention to relationships with others, with the state and the encompassing society.

The Human Mitochondrial Transcriptome

The human mitochondrial genome comprises a distinct genetic system transcribed as precursor polycistronic transcripts that are subsequently cleaved to generate individual mRNAs, tRNAs, and rRNAs. Here, we provide a comprehensive analysis of the human mitochondrial transcriptome across multiple cell lines and tissues. Using directional deep sequencing and parallel analysis of RNA ends, we demonstrate wide variation in mitochondrial transcript abundance and precisely resolve transcript processing and maturation events. We identify previously undescribed transcripts, including small RNAs, and observe the enrichment of several nuclear RNAs in mitochondria. Using high-throughput in vivo DNaseI footprinting, we establish the global profile of DNA-binding protein occupancy across the mitochondrial genome at single-nucleotide resolution, revealing regulatory features at mitochondrial transcription initiation sites and functional insights into disease-associated variants. This integrated analysis of the mitochondrial transcriptome reveals unexpected complexity in the regulation, expression, and processing of mitochondrial RNA and provides a resource for future studies of mitochondrial function (accessed at http://mitochondria.matticklab.com).

Reducing the Background Fluorescence in Mice Receiving Fluorophore/Inhibitor DNA Duplexes

In principle, a DNA duplex consisting of an antisense fluorophore-conjugated major strand hybridized to a shorter complementary inhibitor-conjugated minor strand should provide fluorescence only in the tumor after intravenous administration if designed to remain intact except in the presence in tumor of its mRNA target. While we have obtained impressive tumor images in mice using this approach, there remains some background fluorescence. In this study, tissue homogenates of selected mouse organs were incubated with a test duplex and the kinetics of duplex dissociation in normal tissues were measured. In this manner we were able to identify the liver as the likely major source responsible for the duplex dissociation providing this fluorescence background. Thereafter liver homogenates were used to screen a series of duplex candidates with variable-length minor strands, and dissociation was measured by gel electrophoresis. The selected fluorophore/inhibitor duplex with improved stability displayed an insignificant (P > 0.05) background fluorescence after administration to SKH-1 normal mice and apparently without affecting target mRNA binding in vitro in cell culture or in vivo in tumor bearing mice.

Seismotectonic investigations in the inner Cottian Alps (Italian Western Alps): An integrated approach

This work integrates the results of recent geological–structural studies with new seismological data for the inner Cottian Alps to investigate the connection between faults and seismicity. The major post-metamorphic tectonic feature of this sector is represented by a N–S structure, named Lis–Trana Deformation Zone (LTZ). Since the Late Oligocene, this structure accommodated right-lateral (Late Oligocene–Early Miocene) and subsequently normal (post-Early Miocene) displacements. In the Pleistocene, the activity of the LTZ seems to have caused the development of lacustrine basins inside the valleys that drain this sector of Western Alps. The present-day seismicity joins the northern part of the LTZ and, southwards, other minor sub-parallel structures. In transversal cross-section hypocentres highlight steep surfaces. Focal mechanisms calculated along this structure show both extensional and strike–slip solutions, mostly with one roughly N–S striking nodal plane. Both sub-horizontal (with NE–SW to ENE–WSW trend) and steeply dipping P axes with N–S to NW–SE sub-horizontal T axes are observed. Even if clear evidence of Quaternary tectonic activity in the area is missing, on the basis of the available seismological and geological data we propose that in the inner Northern Cottian Alps the present-day seismic activity may be connected to the LTZ, interpreted as minor sub-parallel fault strand of the Canavese Line. The kinematics of this structure is consistent with the focal mechanisms calculated in this area. Structural and seismological data indicate that LTZ is active under a bulk dextral–transtensive regime since the late Oligocene in the inner Cottian Alps, in agreement with the data published for the adjacent domain of the chain.

The complete mitogenome sequences of the palaeopteran insectsEphemera orientalis(Ephemeroptera: Ephemeridae) andDavidius lunatus(Odonata: Gomphidae)

Currently, the palaeopteran lineages (insect orders Ephemeroptera and Odonata) that have a problematic relationship with neopteran lineages are poorly represented by mitogenome sequences. In this study, we have determined the complete mitogenome of the oriental mayfly, Ephemera orientalis (Ephemeroptera: Ephemeridae), and the dragonfly Davidius lunatus (Odonata: Gomphidae). The 16 463 bp mitogenome of E. orientalis and the 15 912 bp mitogenome of D. lunatus have many of the features typically detected in insect mitogenomes. Although the initiation codon for the D. lunatus COI gene is the typical ATA, E. orientalis is unusual in that no typical start codon was detected in the start region of the COI gene. The A+T-rich regions of both mitogenomes have some unusual features. The E. orientalis A+T-rich region harbors two identical 55 bp sequences separated by 158 bp, and the D. lunatus A+T-rich region harbors a tandem repeat comprising two identical 261 bp copies and one partial copy of the repeat. Additionally, the A+T-rich regions of both mitogenomes harbor the stem-and-loop structures flanked by the conserved sequences "TA(A)TA" at the 5' end and "G(A)nT" at the 3' end, which have been suggested to be the signals involved in minor strand replication initiation. Furthermore, the D. lunatus A+T-rich region contains two tRNA-like structures with proper anticodon and cloverleaf structures.

Optical Antisense Tumor Targeting in Vivo with an Improved Fluorescent DNA Duplex Probe

Fluorescent conjugated DNA oligonucleotides for antisense targeting of mRNA has the potential of improving tumor/normal tissue ratios over that achievable by nuclear antisense imaging. By conjugating the Cy5.5 emitter to the 3' equivalent end of a 25 mer phosphorothioate (PS) antisense major DNA and hybridizing with a shorter 18 mer phosphodiester (PO) complementary minor DNA (cDNA) with the Black Hole inhibitor BHQ3 on its 5' end (i.e., PS DNA25-Cy5.5/PO cDNA18-BHQ3), we previously achieved antisense optical imaging in mice as a proof of this concept. In a process of optimization, we have now evaluated the stability of a small series of duplexes with variable-length minor strands. From these results, a new study anti-mdr1 antisense duplex was selected with a 10 mer minor strand (i.e., PS DNA25-Cy5.5/PO cDNA10-BHQ3). The new study duplex shows stability in serum environments at 37 degrees C and provides a dramatically enhanced fluorescence in KB-G2 (pgp++) cells when compared with KB-31 (pgp+/-) as evidence of antisense dissociation at its mdr1 mRNA target. The duplex was also administered to KB-G2 tumor bearing mice, and when compared to the duplex used previously, the fluorescence from the tumor thigh was more obvious and the tumor-to-background fluorescence ratio was improved. In conclusion, by a process designed to optimize the duplex for optical antisense tumor targeting, the fluorescence signal was improved both in cells and in tumored mice.

Complete Mitochondrial Genome of Teleogryllus emma (Orthoptera: Gryllidae) with a New Gene Order in Orthoptera

The complete sequence of Teleogryllus emma mitochondrial genome was determined using long PCR and conserved primer walking approaches. The results showed that the entire mitochondrial genome of T. emma was 15 660 bp in length, with an A+T content of 74.3%. The most distinct feature of this mtDNA is that the tRNA genes of Glutamic acid, Serine and Asparagine are located in the minor strand, and thus constitutes the third type of gene order of mitochondria in Orthoptera. The intergenic spacers range from 1 to 24 bp in size, totaling 73 bp. A total of 54 overlapping nucleotides exist among 14 genes, ranging from 1 to 11 bp in size. The TAA and TAG codons are used as stop codons in all the protein coding genes (PCGs) except COII, ND5 and ND4. The COI gene has an unusual start codon, TTA, instead of ATN or NTG. Most of the tRNA genes, except tRNASer (AGN), form typical secondary structures. An A+T rich region of T. emma mitochondrial genome with 940 bp in length exists as an ubiquitous secondary structure found in the Orthoptera and other orders of Insecta, such as Diptera, Lepidoptera, Hymenoptera, but not in Collembola, which is considered the basal group of Insecta.

Effects of the Criminalization of HIV Transmission in Cuerrier on Men Reporting Unprotected Sex with Men.

Résumé Cet article dresse un portrait des perceptions et des pratiques des hommes qui ont des rapports sexuels fréquents et non protégés avec d'autres hommes dans un climat sociolégal défini par la décision, en 1998, de la Cour suprême du Canada dans l'affaire R. c. Cuerrier. Depuis cette décision, les personnes séropositives se retrouvent de plus en plus souvent en cour et plusieurs d'entres elles essaient d'incorporer des raisonnements légaux dans leurs propres comportements. La construction judiciaire des comportements à risque en matière de transmission du VIH repose sur un ensemble de présuppositions concernant la responsabilité individuelle, les interactions rationnelles et contractuelles ainsi que les adultes consentants. Ces présuppositions suscitent une série d'ambiguïtés et d'incertitudes chez les personnes séropositives qui essaient de les incorporer dans leur vie quotidienne. Tandis que certaines personnes supportent le raisonnement dans l'affaire Cuerrier, d'autres luttent avec les dilemmes pratiques dans les interactions sexuelles, et une minorité avance un raisonnement éthique qui repose avant tout sur la mise en garde. Ce type de raisonnement est commun dans un environnement social dans lequel les personnes séropositives sentent des pressions les incitant à ne pas divulguer leur séropositivité, d'autant plus qu'elles risquent de faire face à un rejet ou une discrimination suite à cette divulgation. Un examen des conséquences sociales de Cuerrier mène à un questionnement sur la viabilité d'une stratégie de renforcement de la divulgation par la menace d'une poursuite en cour criminelle comme méthode efficace de prévention du VIH, d'autant plus que la prévention de la transmission du VIH a lieu, de façon pragmatique et quotidienne, lorsque les gens se protègent lors des rapports sexuels plutôt que de se fier sur la divulgation.

Roles for TbDSS-1 in RNA surveillance and decay of maturation by-products from the 12S rRNA locus

The Trypanosoma brucei exoribonuclease, TbDSS-1, has been implicated in multiple aspects of mitochondrial RNA metabolism. Here, we investigate the role of TbDSS-1 in RNA processing and surveillance by analyzing 12S rRNA processing intermediates in TbDSS-1 RNAi cells. RNA fragments corresponding to leader sequence upstream of 12S rRNA accumulate upon TbDSS-1 depletion. The 5′ extremity of 12S rRNA is generated by endonucleolytic cleavage, and TbDSS-1 degrades resulting upstream maturation by-products. RNAs with 5′ ends at position −141 and 3′ ends adjacent to the mature 5′ end of 12S rRNA are common and invariably possess oligo(U) tails. 12S rRNAs with mature 3′ ends and unprocessed 5′ ends also accumulate in TbDSS-1 depleted cells, suggesting that these RNAs represent dead-end products normally destined for decay by TbDSS-1 in an RNA surveillance pathway. Together, these data indicate dual roles for TbDSS-1 in degradation of 12S rRNA maturation by-products and as part of a mitochondrial RNA surveillance pathway that eliminates stalled 12S processing intermediates. We further provide evidence that TbDSS-1 degrades RNAs originating upstream of the first gene on the minor strand of the mitochondrial maxicircle suggesting that TbDSS-1 also removes non-functional RNAs generated from other regions of the mitochondrial genome.

Direct infection of potato tubers by the root parasite Orobanche aegyptiaca

SummaryParasitic plants of the Orobanchaceae are known as obligate root parasites that develop haustoria that connect to roots of various host plants. This article describes, for the first time, a case where the root parasite successfully connected to potato tubers, i.e. to the swollen portion of an underground stem rather than to a root. The rhizosphere of Orobanche aegyptiaca and of its host Solanum tuberosum (potato) was carefully examined. In anatomical studies, the adventitious roots were directly connected to potato tubers. Numerous secondary haustoria, which developed along the adventitious roots in close vicinity to the potato tuber, penetrated the tuber epidermis and the perimedullary tuber parenchyma and developed xylem strands that are presumably connected to the minor xylem strands within the tuber cortex. These findings indicate that parasites of the Orobanchaceae that normally attack host roots may also parasitise underground stem tubers.

A Commonwealth of ideas: A valedictory on leaving the headship of the CPSU

At the end of his term as Head of the Commonwealth Policy Studies Unit, the author assesses the strengths and weakness of the organization, arguing that, in a globalized world, the Commonwealth and its agencies are paradoxically not truly global, but trapped in an outmoded geographic framework. He detects signs that this balance of power within the Commonwealth, for decades dominated by the vestiges of Empire and the organization's UK base, is changing. But he warns that the Commonwealth dimension is often a minor strand in international diplomacy.

X-Ray Structures of the Sulfolobus solfataricus SWI2/SNF2 ATPase Core and Its Complex with DNA

SWI2/SNF2 ATPases remodel chromatin or other DNA:protein complexes by a poorly understood mechanism that involves ATP-dependent DNA translocation and generation of superhelical torsion. Crystal structures of a dsDNA-translocating SWI2/SNF2 ATPase core from Sulfolobus solfataricus reveal two helical SWI2/SNF2 specific subdomains, fused to a DExx box helicase-related ATPase core. Fully base paired duplex DNA binds along a central cleft via both minor groove strands, indicating that SWI2/SNF2 ATPases travel along the dsDNA minor groove without strand separation. A structural switch, linking DNA binding and the active site DExx motif, may account for the stimulation of ATPase activity by dsDNA. Our results suggest that torque in remodeling processes is generated by an ATP-driven screw motion of DNA along the active site cleft. The structures also redefine SWI2/SNF2 functional motifs and uncover unexpected structural correlation of mutations in Cockayne and X-linked mental retardation syndromes.

Human DNA polymerase beta initiates DNA synthesis during long-patch repair of reduced AP sites in DNA.

Simple base damages are repaired through a short-patch base excision pathway where a single damaged nucleotide is removed and replaced. DNA polymerase beta (Pol beta) is responsible for the repair synthesis in this pathway and also removes a 5'-sugar phosphate residue by catalyzing a beta-elimination reaction. How ever, some DNA lesions that render deoxyribose resistant to beta-elimination are removed through a long-patch repair pathway that involves strand displacement synthesis and removal of the generated flap by specific endonuclease. Three human DNA polymerases (Pol beta, Pol delta and Pol epsilon) have been proposed to play a role in this pathway, however the identity of the polymerase involved and the polymerase selection mechanism are not clear. In repair reactions catalyzed by cell extracts we have used a substrate containing a reduced apurinic/apyrimidinic (AP) site resistant to beta-elimination and inhibitors that selectively affect different DNA polymerases. Using this approach we find that in human cell extracts Pol beta is the major DNA polymerase incorporating the first nucleotide during repair of reduced AP sites, thus initiating long-patch base excision repair synthesis.

The Comedy of Menander: Convention, Variation and Originality (review)

Reviewed by: The Comedy of Menander: Convention, Variation and Originality David Konstan Zagagi, Netta. The Comedy of Menander: Convention, Variation and Originality. Bloomington and Indianapolis: Indiana University Press, 1995. 210 pp. Cloth, $39.95. In his comedies, Menander exploits a relatively limited range of characters and scenes. His achievement, as Netta Zagagi shows, lies in subtle variations on inherited formulas rather than in radical departures from them. As an example of Menander’s art, Zagagi (32–33) cites the characterization of the young Moschion in Perikeiromene, who is gloriosior than Polemon, the love-sick miles. Again, Chrysis in Samia, because she acts in league with Moschion against his father, “represents a pattern of behaviour traditionally reserved for the character of the mother in comedy” (33). Behind these modifications and mergings of types, Zagagi sees an ethical motivation, best illustrated in Micio’s condemnation of his stepson’s behavior toward the woman he has raped; Terence’s Adelphi, based on Menander’s, is “the only play known to us in which any serious consideration is given to the problematic nature of the story pattern under review” (43). Surely Charisius’ soliloquy in Epitrepontes is another example. Zagagi’s reflections on Menander’s sensitivity to his personae, in contrast to Aristophanes’ delight in caricature (45), are fair enough. It is less clear that Menander differs significantly from the practice of other poets of New Comedy, as Zagagi suggests (32). In the second chapter, Zagagi explains Menander’s method of composition by invoking three principles or techniques that are fundamental to his plays: “polyphony,” “economy,” and “emphasis on human interaction.” These are catch-all terms. “Economy,” for example, refers to Menander’s restraint in the development of scenes that might have “diverted the spectator’s attention from the main subject” of an episode (60). The same term covers Menander’s ability to set out necessary information without undue repetition (62), as well as his thrifty use of devices such as Knemon’s bucket, which first gets dropped in the well and subsequently causes the misanthrope himself to fall in (64). Such dramatic parsimony is the stock-in-trade of well-made plays. Polyphony too, designates three different things. Zagagi ignores Bakhtin’s now classic use of the term to refer to the amalgamation of generic voices characteristic of the novel. Instead, she describes as polyphony the mixture of registers achieved by crossing para-tragic bits of dialogue with the plain style of comedy (54), divine intervention in mortal affairs (57), which is treated in detail in the sixth chapter, and the interweaving of two plot lines in a single play—not contaminatio, precisely, but rather a deft combination of two or more story patterns. [End Page 127] Thus, Dyskolos is analyzed (47) into a “romantic” strand, involving Sostratos’ efforts to win Knemon’s daughter as his bride, and a story line centered on the character of the misanthrope (Zagagi adds for good measure two “minor strands”: the sacrifice to Pan and the Gorgias plot). Zagagi sees as the price of this complexity a loss of dramatic unity: in order to avoid compromising the sketch of Knemon’s grumpiness, Menander does his best to keep the two plots of the comedy separate: “It is precisely from Menander’s constant effort to preserve the credibility of his main hero’s [i.e., Knemon’s] character by shielding him from the romantic action of the play, that the disunity of the Dyskolos stems” (47). This interpretation illustrates a major weakness in Zagagi’s search for elementary units of narrative as the basis of Menandrean composition. Every romantic plot requires not only a lover, but an obstacle or blocking figure (in Northrop Frye’s expression) as well, who, for motives of his own, inhibits the realization of the lover’s passion. In many plays, this function falls to the father of the adulescens, who disapproves of his son’s liaison with a slave-girl or otherwise unsuitable partner. In Dyskolos, the father of the girl (who by the same token is represented as a citizen) plays this role. This is as simple a scheme as one can have, and violates no presumed rule of unity of action. Menander...