In this innovative research I have advanced the theory of space with definition of space, definition of types of space, and introduced energy-mass transformation, gravity and gravitation force. With this advance research, finally I have defined basic mathematical form of “Nature of space and Dimension of Observable Universe and beyond”.As we know the universe is defined as all space-time and their contents. However, several questions still remain open regarding higher dimension, singularity, gravity collapse at horizon of black hole, unified gravity, theory of everything, dark matter, dark energy, existence of observable universe and beyond, question for mathematical form of everything, question for matter itself, and question for the space itself. These open questions have driven this scientific research of “Basic Mathematical form Of Nature of Space and Dimension of Observable Universe and beyond”. My research findings indicate that the space itself is the entity with having physical properties. The space can be described by physical structure and its transformation. The space has two fundamental properties of energy and mass, and space can be described by energy- mass transformation. From these energy-mass transformation and physical structure transformation, I have defined the gravity, gravitation force and basicmathematical form of“Nature of space and Dimension of Observable Universe and beyond”. This basic mathematical form covers scope of higher dimension, unified gravity, singularity, horizon of black hole, and nature of space of observable universe and beyond. Several research have been conductedfor look into nature of space,space-time, gravity, unified gravity, higher dimensions, unified mathematical formof everything by Minkowski space,Higgs field, Aether medium, Newton’s law of universal gravitation, Electromagnetism, Planck constant,General relativity,Standard Model,Loop Quantum Gravity, Super symmetry, Super gravity, String theory and M-Theory. Despite these research, the questions still remain open for unified nature and for unified mathematical form of “Nature of space and Dimension of Observable Universe and beyond”. By researching the existence of matter, I found the existence of space was just accepted as the void, empty, and space-time background framework. My research reflects “Dimension of Observable Universe and beyond”encompasses all existence of observable universe and beyond it. The advance definition of space, definition of type of space, space transformation and energy-mass transformation perfectly describe existence of entire space & universe and its nature at very fundamental level. This very fundamental nature is presented in basic mathematical form. It is the most accurate foundation to answer those open questions. It is the novelty in astrophysics and foundation of entire existence.