Event Abstract Back to Event Insulin and IGF-I effects on the proliferation of an osteoblast primary culture from seabream (Sparus aurata) Encarnación Capilla1*, Laura Acerete1, Isabel Navarro1 and Joaquin Gutierrez1 1 University of Barcelona, Department of Physiology, Faculty of Biology, Spain Bone deformities in several fish species, like seabream (Sparus aurata), are currently a main problem in aquaculture. In order to gain knowledge on fish bone development and skeletal growth, a primary cell culture model has been established from vertebra of seabream. The initial fibroblastic phenotype of the cells changes to a more polygonal shape throughout the culture, and the addition of an osteogenic medium containing ascorbic acid, β-glycerophosphate and calcium chloride, promotes the deposition of minerals in the extracellular matrix (ECM). The capacity of the cells to differentiate into osteoblasts and to mineralize the ECM has been evaluated using von Kossa and Alizarin red staining after three weeks of growing on osteogenic medium. Also, the cells have been induced to differentiate into adipocytes, by the addition of an adipogenic medium consisting on insulin, dexamethasone, IBMX and lipid mixture, and the accumulation of lipids into the cells has been detected with Oil Red O staining, demonstrating the pluripotentiality of these cells. The viability and proliferation of the osteogenic cells has also been analyzed using the MTT assay based on the reduction of the tetrazolium salts under normal and mineralizing conditions at different days along the culture (0, 5, 10, 15 and 20 days). At all times, the cells showed an increased rate of proliferation in the presence of osteogenic medium. Subsequently, the effects of insulin (1, 10 and 100 nM) and IGF-I (0.1, 1 and 10 nM) on cell proliferation have been evaluated at day 3 of the culture in the presence of 2% fetal bovine serum. IGF-I significantly stimulated the proliferation of the cells in a dose response manner, while insulin had only slight effects. Next, the hormonal regulation of osteoblast differentiation will also be analyzed. In summary, a primary culture of seabream osteoblasts has been characterized. This cellular system can be a good model to identify the molecules involved in the process of osteoblastogenesis in fish and its endocrine regulation; and to study bone development to contribute to improve the quality of the product in aquaculture. Supported by the projects from the "Ministerio de Ciencia e Innovación" (MICINN) AGL2008-00783 and AGL2009-12427, the "Xarxa de Referčncia de Recerca i Desenvolupament en Aqüicultura de la Generalitat de Catalunya" and by the project from the European Union LIFECYCLE (EU-FP7 222719). Keywords: comparative endocrinology Conference: 25th Conference of the European Comparative Endocrinologists, Pécs, Hungary, 31 Aug - 4 Sep, 2010. Presentation Type: Conference Presentation Topic: Comparative endocrinology Citation: Capilla E, Acerete L, Navarro I and Gutierrez J (2010). Insulin and IGF-I effects on the proliferation of an osteoblast primary culture from seabream (Sparus aurata). Front. Endocrinol. Conference Abstract: 25th Conference of the European Comparative Endocrinologists. doi: 10.3389/conf.fendo.2010.01.00001 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 25 Aug 2010; Published Online: 29 Aug 2010. * Correspondence: Dr. Encarnación Capilla, University of Barcelona, Department of Physiology, Faculty of Biology, Barcelona, Spain, ecapilla@ub.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Encarnación Capilla Laura Acerete Isabel Navarro Joaquin Gutierrez Google Encarnación Capilla Laura Acerete Isabel Navarro Joaquin Gutierrez Google Scholar Encarnación Capilla Laura Acerete Isabel Navarro Joaquin Gutierrez PubMed Encarnación Capilla Laura Acerete Isabel Navarro Joaquin Gutierrez Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.