Acid Mine Drainage Research Articles

Sulfate-reducing bacteria for bioremediation of sediments from constructed wetlands for acid mine drainage treatment

<p class="AbstractText"><span lang="EN-US">Sediment is a source and sink of heavy metal contaminants in wetlands. In this study, sulfate-reducing bacteria (SRB) were used for the bioremediation of sediments from wetlands affected by acid mine drainage. The sediments were inoculated with SRB </span><span lang="EN-GB">to study their influence on both the solution and sediments.</span><span lang="EN-US"> The changes of pH, electrical conductivity (EC), redox potential (Eh) values, and sulfate (SO<sub>4</sub><sup>2-</sup>), iron (Fe), manganese (Mn), copper (Cu), and zinc (Zn) concentrations in the supernatant were monitored over a period of 70 days. Additionally, the variations in exchangeable, reducible, oxidizable, and residual Fe, Mn, Cu, and Zn concentrations. For the supernatant solution, the pH in all experimental groups decreased slightly within the first 14 days, increased gradually thereafter, and finally stabilized during days 35−70. The EC values and Fe and Mn concentrations displayed a similar trend with concentrations initially increasing and then decreasing. In the SRB-inoculated sediments, Fe, Mn, and Cu were gradually transformed from the exchangeable and reducible fractions to the oxidizable fraction. The inoculation with SRB reduced the ability of Fe, Mn, and Cu to migrate in sediment.</span><span style="font-family:'Times New Roman';font-size:12.0000pt;line-height:200%;mso-fareast-font-family:宋体;mso-font-kerning:1.0000pt;mso-spacerun:'yes';"><o:p></o:p></span></p><p class="MsoNormal" style="line-height:200%;"><span style="font-family:'Times New Roman';font-size:12.0000pt;line-height:200%;mso-fareast-font-family:宋体;mso-font-kerning:1.0000pt;mso-spacerun:'yes';"><o:p></o:p></span></p>

Evaluation of Germination and Early Seedling Growth of Different Grasses Irrigated with Treated Mine Water

Coal mining is known to have negative impacts on the environment, necessitating land rehabilitation after mining activities. Amongst the problems associated with coal mining is the accumulation of acid mine drainage characterized by large amounts of heavy metals and high acidity. The impact of these environmental problems on the ecosystem around mining areas underscores a need to devise strategies that will ensure sustainable restoration of the ecosystem integrity to ensure environmental protection. Of these, treatment of acid mine drainage using calcium sulfate dihydrate, which is subsequently used for irrigation during phytoremediation, holds great promise for restoration of open-cast mines. However, although grasses are used for rehabilitation of coal mined areas, the impacts of treated mine water on the germination, seedling emergence, and plant growth of grasses are not well known. The aim of the study was to evaluate the germination and early seedling growth responses of different forage grasses to treated mine water. Seven forage grass species were selected, with four species represented by two varieties while others were represented by one variety, totaling 11 forage grasses. For each plant entry, 100 seeds were placed in J.R. Petri’s dishes lined with Whatman No. 2 filter paper and watered with distilled and mine water to assess germination. For the seedling establishment experiment, only five species were studied, in which twenty seeds per species were sown in pots containing mine soil and irrigated using distilled and treated mine water. The final germination percentage (FGP), germination rate index (GRI), corrected germination rate index (CGRI), and T50 were determined for the germination trail and total biomass was assessed for the seedling growth trail. The highest FGP for all grasses was attained under controlled conditions, using distilled water, ranging from 38–94%. All grasses germinated when watered using treated mine water and had a FGP ranging from 20–91%. Relative to distilled water, GRI and CGRI were highest only for L. multiflorum cv AgriBoost when seeds were watered using the treated mine water. All grasses watered with treated mine water produced high biomass for the first two weeks, after which biomass production started to decline. Two grasses, Eragrostis curvula cv Ermelo and Lolium multiflorum cv Archie, showed tolerance to treated mine water irrespective of its high electrical conductivity (557 mS∙m−1). Therefore, these grasses could be used in the rehabilitation of coal-mined areas irrigated with treated mine water.

The radiological hazard and potential for generating acid mine drainage from a coal tailings dam.

The aim of this study was to analyze the radiological hazards and the potential for generating acid mine drainage from the fine coal waste commonly stored in tailings dams. The magnetic susceptibility, natural gamma radioactivity, and net neutralization potential of the tailings are characterized. The results show that the fine coal waste has a uranium equivalent concentration (eU) of 46-48Bqkg-1, which is 37.14% higher than the world average, and 39-47Bqkg-1 equivalent concentration of thorium (eTh), which is 56.66% higher than the world average. Also, the absorbed gamma radiation dose rate is higher than the world average. Acid-base balance tests indicate that the net neutralization potential ranged from 0.38 to 2.44. The physical properties indicate a possible radiological risk, while the chemical properties show that generating and non-generating acid drainage can coexist in the fine dam tailings.

Investigation on the Efficiency and Mechanism of Iron and Manganese Removal from Acid Mine Drainage Using Serpentine-Loaded Manganese Oxide

Investigation on the Efficiency and Mechanism of Iron and Manganese Removal from Acid Mine Drainage Using Serpentine-Loaded Manganese Oxide

Mine Water Safety and Environment: Chinese Experience

Coal occupies a central position in the global energy sector [...]

Selective separation of light and heavy rare earth elements from acidic mine waters by integration of chelating ion exchange and ligand impregnated resin

This study addresses the potential of sourcing Critical Raw Materials (CRMs) using Acidic Mine Waters (AMWs) as a secondary resource. AMWs, often viewed as waste, contain valuable metals like zinc and copper, as well as critical metals like magnesium and cobalt. Moreover, recent studies also reported the presence of Rare Earth Elements (REEs) at concentrations (mg/L) that make their extraction both technically and economically viable. The research focuses on a circular process to recover these metals from AMWs, specifically from the Aznalcóllar open-pit mine, which contains 216 mg/L of Al, 47 mg/L of Fe, 547 mg/L of Zn, and 18.56 mg/L of REEs. The proposed method involves pre-treating the AMW to remove Fe and Al, achieving removals of over 99.9 % and 90 %, respectively, at pH 4.5. Following this, transition metals like Zn, Cd, and Cu were removed as sulphides with a removal efficiency exceeding 99 %. This pre-treatment step reduced the concentration of competing metals in the ion-exchange process, thereby enhancing the recovery and purity of REEs. To separate heavy and light REEs, two types of resins in series were used: an impregnated resin (TP272) and a chelating resin (S930), which can be regenerated using sulphuric acid (H2SO4). The final recovery of REEs as oxalates was achieved using oxalic acid and ammonia at pH 1, with further optimization of the elution process to minimize ammonia consumption and undesired precipitation of other oxalates. Finally, REE oxalates with purities exceeding 90 % were obtained. This research demonstrates a sustainable method for efficiently recovering valuable REEs from AMWs, while also addressing environmental concerns related to hazardous sludge generation.

Semi-continuous cultivation of microalgae to treat coal mine effluent in pilot scale: Nutrient removal, biodesalination and fatty acid composition analysis

Coal excavation is associated with discharge of an enormous amount of mine water rich in salt and trace metal ions, which require efficient treatment before use. Considering the potentiality of microalgae for high salt tolerance, salt removal and metallic pollutant removal from aqueous system, present study focuses semi-continuous cultivation of candidate microalgae Chlorella pyrenoidosa (NCIM 2738) in bubble column reactor (BCR) and open raceway pond (ORP) for nutrient supplemented coal mine effluent (NSCME) treatment. In between different hydraulic retention times (HRTs), HRT 6 d provide maximum average biomass productivity of 950 mg L−1 d−1 in BCR and 728.4 mg L−1 d−1 in ORP. HRT 9 d facilitates a maximum lipid content of 1.8 g L ̶ 1 in BCR and 1.4 g L ̶ 1 in ORP. Further, HRT 9 d had the maximum COD removal efficiency (96.5 % in BCR and 94.2 % in ORP) and maximum salinity removal efficiency (93 % in BCR and 92 % in ORP). Fatty acid methyl esters (FAME) characterization highlighted potential biodiesel applicability with cetane number (CN) of 53.94. Energy dispersive X-ray spectroscopy (EDS) revealed an excess amount of salt and metal ions deposition on the surface of harvested microalgae samples. Outdoor large-scale C. pyrenoidosa cultivation can be integrated with coal mine closure plans to meet sustainable development goals (SDGs) 6, 7 and 13.

Passivation of metal sulfides by a marine bacterium for acid mine drainage control

Acid mine drainage originates from metal sulfides oxidation, which results in acidic metal-rich leachate. In this study, a novel and environmentally friendly approach was demonstrated to passivate pyrite and lead-zinc tailings, respectively. The key to this approach is to develop biofilms of the marine bacterium Qipengyuania flava S1. Biofilms can induce biomineralization, thereby isolating metal sulfides from air and water. The stability and biological toxicity of the bio-passivation layers were evaluated by leaching bio-passivated pyrite or tailings in initially acidic H2O2 solutions with shaking for 180 days and then cultivating Brassica chinensis and Allium cepa with the leachates. Our results showed that after passivation, the amount of iron released by pyrite decreased by at least 99.2 ± 0.2 (in wt%). For lead-zinc tailings after passivation, the released metal ions (Fe+Al+Pb+Zn) decreased by at least 52.0 ± 3.2 (in wt%). The bio-passivation layers also maintained the pH of the leachate in the range of 7.5–8.0. Before bio-passivation, compared with mineral water, the pyrite leachate significantly inhibited the growth of the two plants, and the tailings leachate significantly inhibited the growth of A. cepa, whereas the bio-passivated pyrite or tailings leachate did not show any inhibitory effect.

Two-dimensional numerical analysis of seepage in an undersea mining area considering hydrochemical corrosion

The complex hydrogeological conditions and unique hydrochemical environment in the seabed rock often cause serious mine water inrush during undersea mining. With the exploitation of the undersea gold mining area in Sanshandao as the research background, a numerical analysis method for the seepage behavior of the surrounding rock in undersea gold mining considering the hydrochemical corrosion was put forward. A numerical simulation analysis of the undersea mining area in Sanshandao was conducted by the established method, and the results showed that mining the submarine ore body significantly changed the seepage field of the surrounding rock around the stope and water levels dropped obviously in the ore-controlling fault zone. Differences in the hydrochemical environment significantly influenced the distribution of the seepage field of the surrounding rock. The calculation results based on three fluids with different pH values show that the head variation range is the largest when pH = 2, followed by pH = 4, and pH = 7 has the smallest head variation range. The water inflow in the stope was also directly affected by the hydrochemical environment. With the change of pH, the water inflow at each boundary of the stope increases exponentially.

Gradient of acid mine drainage regulates microbial community assembly and the diversity of species associated with native plants

Acid mine drainage (AMD) is considered as one of the most important global environmental challenges. Therefore, understanding the impact of AMD on the diversity of microbial communities associated with native plants is important for phytoremediation. In this study, the community assembly and microbial diversity associated with native plants growing along an AMD impact gradient was investigated using metabarcoding and high throughput iChip technique. The study revealed that across both domains of bacteria and fungi, richness and species diversity decreased according to AMD impact. Bacterial species diversity was more stratified according to the pH gradient than fungi, and the AMD impact on the plant-associated microbial diversity decreased towards the plant roots. The microbial community composition of the undisturbed site was significantly different from the AMD impacted sites, and the communities in the AMD impacted sites were further stratified according to the degree of impact. The overall microbial diversity was mediated by the AMD impact, niche differences and plant species differences. Dispersal limitation was the most important community assembly process in the undisturbed site, while the homogenous selection of Burkholderia, Actinospica, Puia and Bradyrhizobium increased along the AMD impact gradient. Differential abundance analysis further revealed that Umbelopsis, Burkholderia and Sphingomonas were among the biomarkers of the AMD impacted sites. Several strains of some of these responsive genera were subsequently isolated using the iChip. Overall, this study presents novel insight into the ecology of plant-associated microbial communities that are relevant for environmental monitoring and for enhancing the revegetation of AMD impacted sites.

Mechanisms of manganese-tolerant Bacillus brevis MM2 mediated oxytetracycline biodegradation process

Addressing the environmental threat of oxytetracycline (OTC) contamination, this study harnesses the bioremediation capabilities of Bacillus brevis MM2, a manganese-oxidizing bacterium from acid mine drainage. We demonstrate the strain's exceptional efficiency in degrading OTC under high manganese conditions, with complete removal achieved within 24 h. The degradation is facilitated by the production of Bio-MnOx, utilizing their high redox potential and large specific surface area, which significantly enhance the adsorption and oxidation of OTC. Advanced characterization techniques, including X-ray diffraction, scanning electron microscopy, High Resolution-Transmission Electronic Microscope and X-ray photoelectron spectroscopy, provide a detailed analysis of the structural and functional properties of Bio-MnOx. The study also reveals the crucial role of Mn(III) intermediates and reactive oxygen species in the OTC degradation process, with quenching experiments validating their substantial impact on efficiency. Laccase activity, a key manganese-oxidizing enzyme, is assessed spectrophotometrically, further highlighting the enzymatic contribution to Mn(II) oxidation and OTC breakdown. This research contributes valuable insights and approaches for the targeted bioremediation of OTC-contaminated aquatic environments, offering a promising strategy for combating pollution from antibiotics and analogous compounds.

Diffusion Mechanism of Variable-Rate Grouting in Water Prevention and Control of Coal Mine

Regional grouting treatment is an effective technical means to prevent mine water disasters, and the grouting effect is affected by many factors. In actual grouting engineering, the single constant-rate grouting method is often transformed into a variable-parameter grouting process. However, research on grouting rates has been insufficient. This investigation focused on the issue of “the diffusion law of variable-rate grouting slurry in regional governance”. Methods such as theoretical analysis, numerical simulation, and field verification were used to evaluate the diffusion mechanism of variable-rate fracture grouting. The results indicated that the key parameters of variable-rate grouting, such as slurry diffusion distance and grouting pressure, were affected by the grouting rate. The decrease in the grouting rate reduced the migration speed of the slurry and the grouting pressure. The time for constant-velocity grouting and variable-velocity grouting to reach the same diffusion distance was 60 s and 108 s, respectively, which can be achieved with lower grouting pressure. When the grouting rate was 7.5 L/min and 30 L/min, the maximum grout diffusion distance was 2.81 m and 5.64 m, respectively, which required greater grouting pressure. The slurry diffusion rate decreased with the reduction in the grouting rate. Under the same diffusion distance conditions, variable-rate grouting took longer than constant high-rate grouting. In variable-rate grouting, the grouting pressure decreased stepwise with the grouting rate, with a final pressure drop of 77.4%. In grouting practice, the innovative use of the rate-reducing grouting method can greatly reduce the final grouting pressure under the premise of changing the slurry diffusion distance less, which can not only ensure the stability of surrounding rock but also reduce the cost of high-pressure grouting and the risk of grouting operation. The investigation results can provide scientific guidance for ground grouting renovation projects in deep coal mine water hazard areas.

Multicriteria risk assessment of water inrush in underground mines with large-scale curtain grouting: a mine disaster risk reduction strategy

This paper presents a synthetic method to assess the risk of mine water inrush before and after large scale curtain grouting based on GIS platform. Firstly, nine factors were selected from three aspects: geological conditions, hydrogeological conditions and curtain grouting parameters, to construct a multi factor assessment model. The combined weight of every factor was determined using the minimum deviation comprehensive weighting method, which can reduce the polarization effect of various assessment methods in multicriteria decision. Then, the effectiveness of the synthetic method was validated through the Maoping lead–zinc mine in Southwest China. The mining area was divided into five zones, namely safe zone, relatively safe zone, transitional zone, relatively dangerous zone, and dangerous zone. The assessment results show that the relatively dangerous and dangerous zones were significantly reduced, while the safe, relatively safe and transitional zones were significantly increased after large scale curtain grouting. Finally, we analyze the impact of curtain grouting on the risk of mine water inrush and the mechanism of zoning evolution from three aspects: water source control, pathway sealing, drainage, and pressure reduction. This work provides an effective strategy for mine disaster risk reduction.

Concurrent removal of Fe(II), Cu(II), and Zn(II) cations from acid mine drainage by an industrial solid waste - steel slag: Behaviors and mechanisms

Acid mine drainage (AMD) contamination poses a severe environmental threat and is a significant risk to human health. There is an urgent need to develop environmentally sustainable and technically viable solutions for water contamination caused by heavy metals. In this study, steel slag (SS) was used as a secondary resource to concurrently remove Fe(II), Cu(II), and Zn(II) from AMD. Because of the loose and porous structure, abundant functional groups, fast sedimentation velocity, and excellent solid-liquid separation, SS showed exceptional removal performance for heavy metal ions. The adsorption kinetic data of Fe(II), Cu(II), and Zn(II) showed good regression with the pseudo-second-order model. Besides, the adsorption of Fe(II) by SS conformed to the Freundlich model, whereas the adsorption of Cu(II) and Zn(II) followed the Langmuir model, with the maximum adsorption amounts of Cu(II) and Zn(II) being 170.69 and 155.98 mg/g. Furthermore, competitive adsorption was observed among Fe (II), Cu (II), and Zn (II) in a multi-component system, with the adsorption priority being Fe (II) > Cu (II) > Zn (II). The removal mechanism of Fe(II), Cu(II), and Zn(II) in AMD by SS mainly includes electrostatic attraction, chemical precipitation, and surface complexation. Interestingly, the leached concentrations of Fe(II), Cu(II), and Zn(II) from the spent slag after calcination were all within the detection limit of the Chinese emission standard, demonstrating excellent environmental stability. Theoretically, this renders it a viable candidate for use as an additive in construction materials. Meaningfully, the work offers a practical approach for energy-efficient and eco-friendly heavy metal ions adsorption, and the secondary utilization of SS also contributes to the sustainable development of the steel industry. It is beneficial to implement the development concepts of clean production and efficient utilization of industrial solid waste.

Exploring acid mine drainage treatment through adsorption: a bibliometric analysis.

Discharge of acidic wastewater from mining activities (acid mine drainage (AMD)) is a major global environmental and public health issue. Although several approaches, including chemical precipitation and membrane technology, have been developed to treat AMD, adsorption has emerged as the most promising technology due to its cost-effectiveness and efficacy. Despite the wide adoption of adsorption in treating AMD, the evolution of research in this area remains poorly understood. To address this gap, a bibliometric analysis of the most recent literature involving the application of adsorption in AMD remediation was conducted by merging datasets of articles from Scopus (1127) and the Web of Science Core Collection (1422), over the past decade (2013-2022). This analysis revealed a yearly increase of 11% in research publications, primarily contributed by China, the United States, and South Africa. Keyword analysis revealed that natural schwertmannites and their transformations, activated carbon, zeolites, and clay minerals, are the most extensively employed adsorbents for the removal of common metals (arsenic, chromium, iron, manganese, among others). The findings underscore the need for future focuses on recovering rare earth elements, using nanoparticles and modified materials, pursuing low-cost, sustainable solutions, integrating hybrid technologies, pilot-scale studies, exploring circular economic applications of AMD sludges, and inter-continental collaborations. These insights hold significant future implications, serving as a valuable reference to stakeholders in the mining industry.

First full-scale application of barium carbonate as an effective dispersed alkaline substrate for sulfate removal from acid mine drainage

A full-scale passive treatment plant using barium carbonate (BaCO3), the mineral witherite, as a Dispersed Alkaline Substrate (DAS) for strongly contaminated acid mine drainage was implemented for the first time globally at Mina Concepción, located in the Iberian Pyrite Belt (SW Spain). The plant was monitored over a 105-day period, covering the wettest months of the hydrological year and with a mean flow around 4 L s−1. The AMD treated in the plant exhibits a high strength, with an average sulfate concentration around 1500 mg L−1 and net acidity close to 1000 mg L−1 as CaCO₃ equivalent. According to the above, the loading rate for SO4 and Fe was around 400 and 80 kg day−1, respectively. The treatment process produced an alkaline effluent with low metal content. Nearly complete removal of most metal(loid)s was achieved, with significant sulfate decrease to below 500 mg L−1 in the alkaline outflow of the barium carbonate tank. Barium carbonate demonstrated superior performance compared to magnesia, particularly in enhancing alkalinity and lowering net acidity and concentrations of sulfate and manganese. The high efficiency attained by the plant after the barium carbonate treatment is evidenced by compliance with environmental water quality standards for most contaminants.

Applewood Biochar at Different Smoldering Conditions Passivates Pyrite by Promoting the Formation of Jarosite.

To control acid mine drainage (AMD) from the source, a new environmentally and green passivator (biochar) has been introduced to passivate pyrite. To reduce the difficulty of biochar preparation and cost, and improve its production scale, in-situ pyrolysis of applewood by smoldering to produce biochar. Here, particle size, moisture content and gas flow rate were selected to prepare biochar by smoldering through orthogonal combination, and the pyrite was passivated with different conditions and biochar concentrations (2g/L, 3g/L, 4g/L). The results revealed that when the particle size is 200mm×200mm×20mm, the water content is 20-30%, and the gas flow rate is 0.4L/m3, the biochar yield is the highest. Biochar promotes the formation of passivating layer (jarosite), inhibits the release of metal ions. Increasing biochar concentration can promote the formation of jarosite and enhance the passivation effect on pyrite.

The potential of coal mine voids for clean water sources in Nusantara Capital City

PT. Singlurus Pratama operates four mining blocks: Sungai Merdeka, Argosari, Margomulyo, and Mutiara. he Sungai Merdeka block has three voids with significant potential as a source of clean water for Nusantara Capital City (IKN), given its location within the city's development area. Surface water flows from the southern ridge to the north, accumulating in the voids of the Sungai Merdeka block, with a total inflow of 449,218,750 m³ per day. This volume can meet the needs of 1,796,875 to 4,492,187 people. One of the geological formations of the Sungai Merdeka block is the Balikpapan Formation, composed of alternating sandstone and clay with intercalations of shale and limestone, which is likely an aquifer. The water quality in voids 1 and 2 falls into the fairly good category (WQI = 70.07 and 70.77), while void 3 has moderate quality (WQI = 64.76). Thus, the water from the Sungai Merdeka voids can be used for personal and household hygiene, as well as raw water for drinking. Additionally, it can be utilized for recreational water facilities, freshwater aquaculture, livestock, and irrigation. However, the void water in the Sungai Merdeka block is indicated to have formed acid mine drainage, as the exposed void walls are contaminated with oxygen and leached by water. This leads to increased acidity, as indicated by the low pH values of the water. The increased acidity also results in higher concentrations of dissolved metals within the voids, necessitating careful management and treatment to ensure the water’s safety and usability for various applications.

Acidophilic sulphate-reducing bacteria: Diversity, ecophysiology, and applications.

Acidophilic sulphate-reducing bacteria (aSRB) are widespread anaerobic microorganisms that perform dissimilatory sulphate reduction and have key adaptations to tolerate acidic environments (pH <5.0), such as proton impermeability and Donnan potential. This diverse prokaryotic group is of interest from physiological, ecological, and applicational viewpoints. In this review, we summarize the interactions between aSRB and other microbial guilds, such as syntrophy, and their roles in the biogeochemical cycling of sulphur, iron, carbon, and other elements. We discuss the biotechnological applications of aSRB in treating acid mine drainage (AMD, pH <3), focusing on their ability to produce biogenic sulphide and precipitate metals, particularly in the context of utilizing microbial consortia instead of pure isolates. Metal sulphide nanoparticles recovered after AMD treatment have multiple potential technological uses, including in electronics and biomedicine, contributing to a cost-effective circular economy. The products of aSRB metabolisms, such as biominerals and isotopes, could also serve as biosignatures to understand ancient and extant microbial life in the universe. Overall, aSRB are active components of the sulphur and carbon cycles under acidic conditions, with potential natural and technological implications for the world around us.

The treatment of acid mine drainage (AMD) using a combination of selective precipitation and bio-sorption techniques: A hybrid and stepwise approach for AMD valorization and environmental pollution control

In this study, selective precipitation using magnesium oxide (MgO) and bio-sorption with banana peels (BPs) were explored for the treatment and valorization of acid mine drainage (AMD). The treatment chain comprised two distinct stages of which selective precipitation of chemical species using MgO (step1) and polishing of pre-treated AMD using BPs (step 2). In stage 1, 2.0 L of AMD from coal mine were used for selective precipitation and recovery of chemical species using MgO. The results revealed that chemical species of concern were precipitated and recovered at different pH gradients with Fe(III) precipitated at pH ≤ 4, Al at pH ≥ 4-5, Fe(II), Mn and Zn at pH ≥ 8 while Ca and SO42─ were precipitated throughout the pH range. In stage 2, the pre-treated AMD water was polished using BPs. The results revealed an overall increase of pH from 1.7 to 10, and substantial removal of chemical species in the following removal efficiency: Al, Cu and Zn (100% each), ≥ Fe and Mn (99.99% each), ≥ Ni (99.93%), and ≥ SO42─ (90%). The chemical treatment step removed pollutants partially, whereas the bio-sorption step acted as a polishing stage by removing residual pollutants.