Minimum Inhibition Zone Research Articles

Murexide retention and antibacterial performance of semiconductor copolymer/magnetic iron oxide hybrid nanocomposites synthesized via one pot in-situ chemical oxidative polymerization

Herein, semiconductor-magnetic hybrid nanocomposites, poly(vanillin-co-thiophene)/magnetic iron oxide (PVTO-Fe3O4), were successfully synthesized via single-step in situ chemical oxidative polymerization in the presence of different weight percentages of magnetic iron oxide nanoparticles Fe3O4 NPs (3, 6, and 9 wt%). Whereas the Fe3O4 NPs were prepared via an environmentally friendly, modified co-precipitation method, and their physicochemical characteristics were comprehensively analyzed using various analytical techniques. The adsorption behavior of the resulting samples towards murexide dye in aqueous solutions was investigated, along with their antibacterial efficacy against both gram-positive (Staphylococcus aureus and Bacillus) and gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. The adsorption study revealed that the nanocomposite containing 9 wt% Fe3O4 NPs achieved the highest removal efficiency for MX (approximately 87.41 %) and exhibited a maximum adsorption capacity of approximately 100.7 mg/g, surpassing many adsorbents reported in the literature. Optimization experiments identified the optimal conditions as 40 mg of adsorbent dose, 40 mL of MX solution (125 mg/L), pH 5, and 160 min contact time. The adsorption kinetics followed pseudo-second-order kinetics, and the adsorption isotherm fitted well with the Freundlich model. Moreover, the nanocomposite was efficiently recovered from the solution using an external magnet, highlighting its potential for practical applications. Antibacterial testing revealed significant antibacterial effectiveness of PVTO and its nanocomposites against both gram-positive and gram-negative bacteria, with a minimum inhibition zone of 20 mm.

Phytochemical Profiling and Antibacterial Dynamics of Xanthium strumarium L Extract: Unlocking Potential Therapeutic Pathways

This investigation focuses on the analysis of the chemical constituents and possible therapeutic effects of extracts derived from botanical sources. The research entails a quantitative evaluation of phenolic compounds, alkaloids, carotenoids and tannins present in an ethanolic extract from the aerial components of the plant Xanthium strumarium L. Analytical results indicate a considerable presence of these phytochemicals within the extract, with phenolics, alkaloids and carotenoids as predominant constituents. Phenolic compounds represent 19% of the extract, notable for their role in antioxidative activities which are critical in scavenging free radicals and may contribute to lowering the incidence of chronic diseases. Alkaloids, accounting for 12% of the extract, have been recognized for their wide-ranging pharmacological actions. Carotenoids, which constitute 25% of the extract, suggest the extract's potential as a nutraceutical agent for health enhancement and disease prophylaxis. The study underscores the significant levels of these bioactive molecules, reflecting a phytochemical matrix with potential for antioxidative, anti-inflammatory and extensive pharmacological utilities. Additionally, the manuscript investigates the in vitro antibacterial efficacy of the Xanthium extract against various bacterial pathogens. By assessing the extract's minimum inhibitory concentration (MIC) and zone of inhibition (ZOI) and comparing these parameters to those of tetracycline, a standard antibiotic, the research delineates a concentration-dependent antibacterial activity of the extract. It is noteworthy that an increase in the extract's concentration resulted in a reduction in MIC and an augmentation in ZOI, particularly against E. coli and S. aureus, suggesting the extract's potential as an antibacterial agent.

A novel zingerone-loaded zinc MOF coated by niosome nanocomposites to enhance antimicrobial properties and apoptosis in breast cancer cells

Breast cancer (BC) is a prevalent malignancy among women, presenting significant health risks. The development of targeted smart drug delivery has greatly enhanced tumor therapy and antibacterial treatments. In this study, we fabricated a novel Zn-metal organic framework (Zn-MOF) at the nanoscale using an oil bath technique and loaded it with zingerone (ZG) and coated with niosomes (Nio) to form ZG-Zn-MOF@Nio, designed for both anti-microbial and anticancer purposes. We investigated the anti-microbial activity by assessing the minimum inhibitory concentration and zone of inhibition by zingerone, Zn-MOF, and ZG-Zn-MOF@Nio. Scanning electron microscopy (SEM) analysis revealed that the synthesized ZG-Zn-MOF@Nio exhibited well-defined rod and cilia-like morphology. Transmission electron microscopy (TEM) images showed ZG-Zn-MOF@Nio nanoparticles (NPs) with a spherical-like shape and an average diameter of 0.34 µm and a zeta potential of (+22 mV). The encapsulation efficiency of zingerone into Zn-MOF was up to 92.56 %, with a loading capacity of 11.55 % (*P<0.05). Four kinetic models were used to analyze the release mechanism of zingerone at pH 6.5, with the zero-order model showing an excellent fit (R2 = 0.9985, *P<0.05). Additionally, the minimum inhibitory concentration of synthesized zingerone, Zn-MOF, and ZG-Zn-MOF@Nio NPs were tested against Gram-positive Bacillus subtilis (B. subtilis) and Staphylococcus aureus (S. aures), as well as the gram-negative strains Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa). In vitro test revealed that the minimum inhibitory concentration of ZG-Zn-MOF@Nio was 31.25 μg/mL against S. aures and B. subtilis, and 62.5 μg/mL against P. aeruginosa and E.coli were. The MCF-7 cell line was utilized as in-vitro model. The MTT assay demonstrated significant cytotoxicity of ZG-Zn-MOF@Nio against MCF-7 cells, with an IC50 values of 46.2 µg/mL, inducing apoptosis in 94.8 % of the cells.

Biosynthesis of iron oxide nanoparticles using plant extracts and evaluation of their antibacterial activity

The biosynthesis of iron oxide nanoparticles has received increasing attention in the field of food nanotechnology because of their non-toxicity, high efficiency, high antibacterial power, and decontamination features. Therefore, biosynthesis of iron oxide nanoparticles (nFe) was prepared from the leaves of some vegetables, such as cabbage (C) and turnips (T), as well as moringa leaves (M). Alcoholic extracts of these nanoparticles were also tested on Staphylococcus aureus and Escherichia coli to evaluate their antibacterial activity. The results revealed that the particle sizes of the biosynthesis nanomaterials studied ranged from 12.99 to 22.72 nm, and the particles were spherical, irregular, and surrounded by black color. It also contains many functional groups and minerals. Iron nanoparticles modified with Moringa oleifera extract at a concentration of 200 ppm had the highest phenol content compared to other biosynthesis nanoparticles studied. TnFe and MnFe at 200 ppm had a maximum zone of inhibition of 25 mm and 24 mm against Staphylococcus aureus and Escherichia coli, respectively. While the minimum inhibition zone of 8.0 mm was observed at 25 ppm for nFe against Escherichia coli. Therefore, it is recommended to use these extracts of biosynthesis iron oxide nanoparticles as antibacterial agents for stored foods.

Fabrication and optimization of naringin-loaded MOF-5 encapsulated by liponiosomes as smart drug delivery, cytotoxicity, and apoptotic on breast cancer cells

Introduction Cancers are regarded as hazardous due to their high worldwide death rate, with breast cancer (BC), which affects practically all cancer patients globally, playing a significant role in this statistic. The therapeutic approach for BC has not advanced using standard techniques, such as specialized naringin (NG) chemotherapy. Instead, a novel strategy has been utilized to enhance smart drug delivery (SDD) to tumors. Significance Herein, we established NG-loaded zinc metal-organic framework-5 (NG-MOF-5) coated with liponiosomes (LNs) to manufacture NG-MOF-5@LNs nanoparticles (NPs) for antibacterial and cancer treatment. Methods MOF-5, NG, and NG-MOF-5@LNs were evaluated with XRD, thermogravimetric analysis (TGA), FTIR, SEM, TEM, PDI, ZP, encapsulation efficiency (EE), loading efficiency (LE), and drug release (DR) kinetics. We examined the antibacterial activity involving minimum inhibitory concentration (MIC) and zone of inhibition by NG, MOF-5, and NG-MOF-5@LNs. The cell viability, necrosis, and total apoptosis (late and early) were evaluated for anti-cancer activity against MCF-7 BC cells. Results TEM results demonstrated that NG-MOF-5@LNs formed monodispersed spherical-like particles with a size of 122.5 nm, PDI of 0.139, and ZP of +21 mV. The anti-microbial activity results indicated that NG-MOF-5@LNs exhibited potent antibacterial effects, as evidenced by inhibition zones and MIC values. The Higuchi model indicates an excellent fit (R 2 = 0.9988). The MTT assay revealed anti-tumor activity against MCF-7 BC cells, with IC50 of 21 µg/mL for NG-MOF-5@LNs and demonstrating a total apoptosis effect of 68.2% on MCF-7 cells. Conclusion NG-MOF-5@LNs is anticipated to show as an effective antimicrobial and novel long-term-release antitumor agent and might be more suitable for MCF-7 cell therapy.

Bio-Activity of Crude Alcoholic Extracts of Buffel Grass in different Solvents against some Pathogenic Microbes

Background: Natural products have long been the focus of researchers aiming to improve human health, develop medicines with minimal adverse effects, and extend human lifespans. These products, derived from plants, animals, marine life, and microorganisms, contain secondary metabolites known as phytochemicals, which exhibit various biological activities. The current investigation aimed to assess the phytochemical properties and antimicrobial potential of Cenchrus ciliaris Linn. (CAZRI-358) against significant human pathogenic bacteria and fungi. Objective: The objective of this study was to conduct a phytochemical analysis and evaluate the inhibitory potential of crude alcoholic extracts of Cenchrus ciliaris Linn. against six important human pathogenic bacteria and three fungal strains. Methods: The plant material of Cenchrus ciliaris was shade-dried, crushed into powder, and successively extracted with methanol (CCWPM), hexane (CCWPH), and chloroform (CCWPC) using Soxhlet extraction. The antimicrobial activity of the extracts was assessed using both disc diffusion and serial dilution methods. The minimum inhibitory concentration (MIC) and zone of inhibition (IZ) were calculated. The bacterial strains tested included Shigella sonnei, Pseudomonas aeruginosa, Escherichia coli (Gram-negative), Staphylococcus aureus, Streptococcus pyogenes, and Bacillus subtilis (Gram-positive). The fungal strains tested included Microsporum canis, Aspergillus clavatus, and Candida albicans. Standard drugs imipenem (for bacteria) and miconazole (for fungi) were used as positive controls. Results: The highest yield was found in the methanolic extract (41.25 mg/g). Methanolic extracts showed maximum antibacterial activity with MIC values of 90.98 ± 0.05 µg/mL and IZ of 39 mm against Escherichia coli, 92.11 ± 0.06 µg/mL and IZ of 39 mm against Pseudomonas aeruginosa, and 95.33 ± 0.06 µg/mL and IZ of 39 mm against Streptococcus pyogenes. The methanolic extract also exhibited significant antifungal activity with an MIC of 91.97 ± 0.03 µg/mL and IZ of 40 mm against Microsporum canis. Other extracts demonstrated notable but lesser antimicrobial activities. Conclusion: Cenchrus ciliaris Linn. contains vital bioactive components with significant antimicrobial properties, particularly in methanolic extracts. These findings suggest its potential in developing treatments for infections, chronic diseases, and conditions like Benign Prostatic Hyperplasia (BPH). Further research is needed to isolate specific bioactive compounds and understand their mechanisms of action.

Assessment of antimicrobial activity and GC-MS using culture filtrate of local marine Bacillus strains

Secondary metabolites produced by Bacillus species from marine sources encompass a variety of compounds such as lipopeptides, isocoumarins, polyketides, macrolactones, polypeptides and fatty acids. These bioactive substances exhibit various biological activities, including antibiotic, antifungal, antiviral, and antitumor properties. This study aimed to isolate and identify a particular species of Bacillus from marine water and organisms that can produce bioactive secondary metabolites. Among the 73 Bacillus isolates collected, only 5 exhibited antagonistic activity against various viral and bacterial pathogens. The active isolates were subjected to 16S rRNA sequencing to determine their taxonomical affiliation. Among them, Bacillus tequilensis CCASU-2024-66 strain no. 42, with the accession number ON 054302 in GenBank, exhibited the highest inhibitory potential. It displayed an inhibition zone of 21 mm against Bacillus cereus while showing a minimum zone of inhibition of 9 mm against Escherichia coli and gave different inhibition against pathogenic fungi, the highest inhibition zone 15 mm against Candida albicans but the lowest inhibition zone 10 mm was against Botrytis cinerea, Fusarium oxysporum. Furthermore, it demonstrated the highest percentage of virucidal effect against the Newcastle virus and influenza virus, with rates of 98.6% and 98.1%, respectively. Furthermore, GC-MS analysis was employed to examine the bioactive substance components, specifically focusing on volatile and polysaccharide compounds. Based on these results, Bacillus tequilensis strain 42 may have the potential to be employed as an antiviral agent in poultry cultures to combat Newcastle and influenza, two extremely destructive viruses, thus reducing economic losses in the poultry production sector. Bacteria can be harnessed for the purpose of preserving food and controlling pathogenic fungi in both human and plant environments. Molecular docking for the three highly active derivatives 2,3-Butanediol, 2TMS, D-Xylopyranose, 4TMS, and Glucofuranoside, methyl 2,3,5,6-tetrakis-O-(trimethylsilyl) was carried out against the active sites of Bacillus cereus, Listeria monocytogenes, Candida albicans, Newcastle virus and influenza virus. The data obtained from molecular docking is highly correlated with that obtained from biology. Moreover, these highly active compounds exhibited excellent proposed ADMET profile.

Efficacy of edible plant extracts against multi-drug resistant Salmonella serovars in layer flocks

The present study examines the antibacterial effects of edible plants (Neem, Garlic, and Ginger) extracts against Salmonella serovars isolated from layer flocks using phenotypic antimicrobial resistance techniques. The incidence of positive isolates was higher in Dinajpur (13.89%), followed by Thakurgoan (11.91%), Nilphamari (10%), and Panchagarh (8.33%). Organoleptic analysis showed a higher prevalence in the liver (17.86%) compared to the heart (7.14%) and lung (10.72%). Ethanolic extractions were performed from the plants, and the antibacterial activity of these extracts was assessed using the disc diffusion method at concentrations of 80, 100, and 120 mg/mL against multidrug-resistant (MDR) bacteria, which were identified from selected layer flocks using conventional laboratory techniques. These bacteria are resistant to at least two antibiotics. The maximum inhibition zones for Neem, Garlic, and Ginger extracts at 120 mg/mL were 14 mm, 10 mm, and 2 mm, respectively, while the minimum inhibition zones at 80 mg/mL were 10 mm, 3 mm, and none, respectively. Neem leaf extract showed the highest effectiveness against MDR bacteria compared to Garlic and Ginger extracts. Although the organisms were sensitive to ciprofloxacin and colistin, they showed intermediate resistance to levofloxacin and chloramphenicol and resistance to neomycin and kanamycin. When comparing the antibacterial efficacy of resistant antibiotics and plant extracts in vitro, plant extracts showed larger inhibition zones. Therefore, it was concluded that ethanolic Neem leaf extract could serve as an alternative to conventional antibiotics against field isolates of Salmonella spp. in poultry.

Antibacterial potential of lactic acid bacteria isolated from raw cow milk in Sylhet district, Bangladesh: A molecular approach.

The most prevalent probiotic bacterium employed in the food industry is Lactobacillus because it can produce metabolites with antibacterial capabilities and exhibits hostility towards infections and microorganisms that cause spoilage. This study set out to identify naturally occurring Lactobacillus and plantaricin (pln EF) coding genes in raw cow milk and to assess the antibacterial potency of isolated Lactobacillus isolates. Following enrichment in De Man, Rogosa and Sharpe (MRS) broth, single colonies were isolated, and pure colonies were obtained by streaking on MRS agar. The 16S rRNA gene was amplified using polymerase chain reaction (PCR) to confirm the cultural positivity of all isolates. Additionally, the presence of plantaricin was verified by targeting the pln EF gene through PCR. Out of the 166 raw milk specimens acquired from cows, 153 (91.17%; CI: 86.98-95.76) were identified as positive for Lactobacillus through both culture and biochemical screening. Subsequently, 121 (72.89%; CI: 65.46-79.49) of the isolates were affirmed to harbour Lactobacillus through PCR analysis. Within this subset, 6 isolates (4.96%; CI: 1.84-10.48) were found to possess the plnEF gene. When exposed to Lactobacillus isolates, Salmonella Typhimurium and Salmonella enterica displayed an average maximum zone of inhibition with a diameter measuring 24mm. In contrast, Escherichia coli exhibited an average minimum zone of inhibition, featuring a diameter of 11mm. Additionally, the Lactobacillus isolates demonstrated inhibitory zones against Staphylococcus aureus, Klebsiella pneumoniae and Klebsiella oxytoca, measuring 14, 22 and 19mm, respectively. Lactic acid bacteria, particularly Lactobacilli, are plentiful in cow milk and possess broad-spectrum antibacterial properties.

Controlled dual activity of an organometallic antibiotic through micelle formulation

Sodium Lauryl Sulphate (SLS) and Cetyltrimethylammonium bromide (CTAB) were employed to create micelles (SLS@CIPTIN and CTAB@CIPTIN) of the organometallic antibiotic Ph2Sn(CIP)2 (CIPTIN) (where HCIP= ciprofloxacin). The characterization of the micelles was conducted in the solid-state using melting point, Attenuated Total Reflectance-Fourier Transform Infra-Red (ATR-FTIR), 119Sn Mössbauer and X-ray Fluorescence (XRF) spectroscopies, and Thermogravimetry/Differential Thermal Analysis (TG-DTA), while in solution, by Ultra violet-Visible (UV–Vis) and Nucleus Magnetic Resonance (1HNMR ) spectroscopic techniques were employed. The compounds were tested for their antiproliferative activity against human breast adenocarcinoma cells: MCF-7 (hormone depended (HD)) and MDA-MB-231 (hormone independent (HI)). The in vitro and in vivo non-genotoxicity was confirmed with micronucleus (MN) and Artemia salina assay. The antiproliferative mechanism of action of SLS@CIPTIN and CTAB@CIPTIN was studied by cell morphology and cell cycle arrest. The antibacterial activity was evaluated using Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Inhibition Zones (IZ) against Minimum Bactericidal Concentration (MBC) against Gram-negative strains P. aeruginosa, E. coli, as well as Gram-positive strains S. epidermidis and S. aureus. The results indicate that both the investigated CIPTIN and its supramolecular assembly (SLS@CIPTIN) exhibit activity. Therefore, the encapsulation of antimicrobial compounds can indeed be regarded as an attractive strategy for the development of new dual-active agents. These agents offer concurrent anticancer and antibacterial attributes without inducing toxicity.

In‐situ Synthesis of Highly Potent Antibacterial Copper‐Based MOFs/Sodium Alginate Composite Beads

AbstractThe present study reports an environmentally friendly in‐situ synthesis of novel antibacterial copper‐based MOFs within the hydrogel network of sodium alginate. Two different copper‐based MOF/sodium alginate composite beads were prepared via the post‐treatment of copper‐ion‐crosslinked alginate hydrogels with two different ligand solutions, namely, tartaric acid and oxalic acid, at 100 °C for 24 h. The structural, thermal, and morphological properties of the prepared samples were investigated using Fourier transform infrared spectroscopy (FTIR), X‐ray diffraction (XRD), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM), and their antibacterial activities against gram‐positive (Staphylococcus aureus and Bacillus) and gram‐negative (Escherichia coli and Pseudomonas aeruginosa) strains were examined using the conventional disc diffusion method. The results demonstrated the success of the in‐situ synthesis of two distinct copper‐based MOFs with FTIR spectra, confirming the existence of characteristic bands of the ligands complexed to the sodium alginate matrix. Moreover, the XRD diffractograms revealed the formation of two distinct crystalline structures with well‐defined morphologies observed in the SEM images. In addition, thermal analysis showed that the prepared composite beads had enhanced thermal stability compared to the copper‐ion‐crosslinked alginate beads. Antibacterial testing revealed the strong capacity of the copper‐based MOFs/sodium alginate composite beads to deactivate the growth of all the bacterial strains used, with a minimum inhibition zone of 23 mm, which highlights the potential of the synthesized materials as highly potent antibacterial agents.

Antibacterial activity and mechanism of X33 antimicrobial oligopeptide against Acinetobacter baumannii

Acinetobacter baumannii is a pathogenic bacterium widespread in human environments, especially in intensive care units, and is associated with high morbidity and infection rates. Multiple drug resistance in A. baumannii frequently leads to the death of patients, making the development of multi-effect antibacterial agents against this bacterium a research hotspot. We have previously found that the X33 antimicrobial oligopeptide can effectively inhibit the growth of Penicillium digitatum and Candida albicans. Herein, we evaluated the antibacterial activity of X33 antimicrobial oligopeptide against A. baumannii by determining the minimum inhibitory concentration, inhibition zone, and growth curve. The increase in extracellular alkaline phosphatase and the leakage of intracellular compounds confirmed the effect of X33 antimicrobial oligopeptide on the cell wall and membrane. Changes in reactive oxygen species, malondialdehyde, ATP, reducing sugar, soluble protein, and pyruvate content demonstrated that the incubation with X33 antimicrobial oligopeptide affected energy metabolism and oxidative stress. Consistent with the physiological characteristics, transcriptomics analysis indicated that incubation with X33 antimicrobial oligopeptide significantly induced changes in the expression of 2339 genes, including 1262 upregulated and 1077 downregulated genes, which participate in oxidative phosphorylation, ribosome, quorum sensing, fatty acid degradation, glycolysis/gluconeogenesis, and citrate cycle pathways. These results provide a fundamental basis for investigating the mechanism of X33 antimicrobial oligopeptide as a potential drug against A. baumannii.

Phytochemical Constituents and Antimicrobial Activity of Marine Green Seaweed Ulva lactuca

The aim of this study is to determine the presence of Ulva seaweeds, possibly by analyzing the quality of seaweed powder extracts and some organic solvents. It belongs to the order Ulvaales. Ulva lactuca is a widespread macro algae growing the Mediterranean coast phylum Chlorophyta, commonly known as “sea lettuce”. Collected from the Gulf of Mannar Tamil Nadu, India. Dry the Ulva seaweeds and grind it in a food processor until it becomes a fine powder. The powder is dried in an oven at 600c for 24 hours. Alkaloids, Flavonoids, Saponins and Tannins. dried seaweed nutrients rich in energy 252.72kcal/mg/gm, carbohydrates 49.63mg/gm, less protein 12.21mg/gm, fat 1.04mg/gm less than gm, high crude fiber, ash15.8mg/g, high moisture 21.74mg/g. in the UV –visible spectrum of seaweed extract TLC analysis 200-800nm found a high value of0.925, to0.477, GC-MS RF value of8.014, and FTIR analysis of seaweeds Ulva found a high value of 618 to 3525cm-1 and HPLC showed retention time 2.213- 3.730. The antimicrobial studies maximum inhibition zone, minimum concentrations, minimum inhibition zone and maximum activity of Klebsiella pneumoniae, are methanol extract 10mm, Staphylococcus aureus, ethyl acetate extract11mm, Candida albicans 10.5mm were respectively.

Synthesis of New Mn(II), Co(II) and Cu(II) Complexes Grabbed in Novel Functionalized Ionic Liquid Tagged Schiff base: Physico-chemical Properties and Antibacterial Applications

An ionic liquid tagged schiff base, [1-{2-(2-hydroxybenzylideneamino)ethyl}-3-methylimidazolium]PF6 was synthesized from a functionalized ionic liquid (FIL) 2-aminoethyl-3-methylimidazolium hexafluorophosphate, [2-aemim]PF6 and salicylaldehyde without using any organic solvent. The Schiff base was used as a ligand to trap Mn(II), Co(II), Cu(II) ions from its corresponding metal acetates and to form the new transition metal complexes. The synthesized Mn(II), Co(II), Cu(II) complexes along with the Schiff base were characterized by ESI-MS, PXRD, UV-Visible, FT-IR, 1H NMR spectroscopic and analytical (elemental analysis and magnetic susceptibility measurements) methods. Based on these spectral analysis data tetra coordinated geometries were assigned for the newly synthesized metal(II) complexes. Antibacterial sensitivity of the Schiff base and the Mn(II), Co(II), Cu(II) complexes were evaluated against gram positive (Staphylococcus aureus) and gram negative (Escherichia coli) bacteria to assess their minimum zone of inhibition.

Tetracycline: structural characterization and antimicrobial properties of its water-soluble di-anionic bi-sodium salt.

The new water-soluble di-anionic bi-sodium salt of tetracycline (TC), an antibiotic in clinical use, with the formula {[TC]2-[Na+(MeOH)(H2O)] [Na+]·(H2O)}n (TCNa) was synthesized. The compound was characterized by m.p., attenuated total reflectance-Fourier transform infra-red (ATR-FTIR) spectroscopy, and ultraviolet (UV) and proton nuclear magnetic resonance (1H NMR) spectroscopy in the solid state and in solution. The molecular weight (MW) was determined by cryoscopy. The crystal structure of TCNa was also determined by X-ray crystallography. The antibacterial activity of TCNa was evaluated against the bacterial species Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli), Staphylococcus epidermidis (S. epidermidis) and Staphylococcus aureus (S. aureus) by means of minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and inhibition zones (IZs). Moreover, the ability of the compound to eradicate biofilm formation was also evaluated. The results are compared with those obtained for the commercially available drug TCH2. The in vitro and in vivo toxicities of TCNa were tested against human corneal epithelial cells (HCECs) and Artemia salina.

Evaluation of Antioxidant and Antimicrobial Activity of Ethanolic Extract of Medicinal Plants Baccaurea Ramiflora and Microcos Paniculata.

The present study was undertaken to investigate the antioxidant and antimicrobial effect of ethanolic leaf extract of Baccaurea ramiflora and Microcos paniculata. DPPH radical scavenging activity, Nitric oxide scavenging activity, Super oxide anion radical scavenging activity, Reducing power assay were used to assess antioxidant efficacy. Zone of Inhibition determination by Agar well diffusion assay was used to assess antimicrobial activity. The Baccaurea ramiflora and Microcos paniculata leaves extracted with different solvents such as petroleum ether, chloroform, ethanol and water among that in leaves ethanolic extract produce 10.78, 10.38 percentage yield respectively. Both the extracts subjected for phytochemical investigation revealed the presence of alkaloids, glycosides, tannins, saponins, proteins and flavonoids. Ethanolic extract of Baccaurea ramiflora showed maximum inhibition zone diameter was obtained in Salmonella typhi (Gram-negative bacteria) with diameter 29 mm and 25 mm respectively at 200mg/ml and 100mg/ml. Similarly, Ethanolic extract of Microcos paniculata showed minimum inhibition zone diameter compare to Baccaurea ramiflora was obtained in Salmonella typhi (Gram-negative bacteria) with diameter 23 mm and 19 mm respectively at 200mg/ml and 100mg/ml. Ethanolic extract of Baccaurea ramiflora showed maximum inhibition zone diameter was obtained in Aspergillus fumigates with diameter 25 mm and 22 mm respectively at 200mg/ml and 100mg/ml. Similarly, Ethanolic extract of Microcos paniculata showed minimum inhibition zone diameter compare to Baccaurea ramiflora was obtained in Aspergillus fumigates with diameter 21 mm and 19 mm respectively at 200mg/ml and 100mg/ml. The current findings point to Baccaurea ramiflora and Microcos paniculata antioxidant and antimicrobial properties. However future studies should be designed to isolate the active constituents responsible for the specified effect.

Phytochemical Screening and Antimicrobial Activities of Leaves of Bombax ceiba L.

Bombax ceiba L. is a multi-purpose and fast-growing tree belonging to family Bombacaceae. It has astringent, diuretic, anti-diarrheal, etc. properties and also finds its uses in pharmacognosy. The leaves of B. ceiba L. have different phytochemicals like alkaloids, phenols, tannins, saponins, carbohydrates, etc. The leaves were used for phytochemical evaluation and its response against microbes. The extract for qualitative phytochemical analysis, prepared in distilled water, was done to test the existence of different phytochemicals like alkaloids, phenols, tannins, saponins, carbohydrates, etc. present in the leaves. Disc-diffusion method was employed to test the antimicrobial activity in different solvents like Dimethyl sulfoxide (DMSO), Ethanol and Water. Pseudomonas aeruginosa showed maximum zone of inhibition i.e. 25.33mm in the Ethanol extract, and minimum zone of inhibition was observed against Pseudomonas aeruginosa of 6.33mm in DMSO. These findings can help to understand the significance of the plant for preparation of medicine for some specific disease and can also be used to determine the effectiveness of the plant against certain micro-organisms.

Innovative Preparation of Cellulose-Mediated Silver Nanoparticles for Multipurpose Applications: Experiment and Molecular Docking Studies.

In recent years, inorganic metal nanoparticle fabrication by extraction of a different part of the plant has been gaining more importance. In this research, cellulose-mediated Ag nanoparticles (cellulose/Ag NPs) with excellent antibacterial and antioxidant properties and photocatalytic activity have been synthesized by the microwave-assisted hydrothermal method. This method is a green, simple, and low-cost method that does not use any other capping or reducing agents. X-ray diffraction (XRD), Fourier transform infrared (FTIR), field emission scanning microscopy (FESEM), transmission electron microscopy (TEM), energy-dispersive X-ray (EDX), and UV-visible spectroscopic techniques were used to investigate the structure, morphology, as well as components of the generated cellulose/Ag NPs. In fact, XRD results confirm the formation of the face-centered cubic phase of Ag nanoparticles, while the FTIR spectra showed that the synergy of carbohydrates and proteins is responsible for the formation of cellulose/Ag NPs by the green method. It was found that the green-synthesized silver nanoparticles showed good crystallinity and a size range of about 20-30 nm. The morphology results showed that cellulose has a cavity-like structure and the green-synthesized Ag NPs were dispersed throughout the cellulose polymer matrix. In comparison to cellulose/Ag NPs and Ag nanoparticles, cellulose/Ag NPs demonstrated excellent antibacterial activity, Proteus mirabilis (MTCC 1771) possessed a maximum inhibition zone of 18.81.5 mm at 2.5 g/mL, and Staphylococcus aureus (MTTC 3615) had a minimum inhibition zone of 11.30.5 mm at 0.5 g/mL. Furthermore, cellulose/Ag NPs also exhibited a significant radical scavenging property against the DDPH free radical, and there was a higher degradation efficiency compared to pure Ag NPs against Rhodamine B as 97.38% removal was achieved. Notably, cellulose/Ag NPs remarkably promoted the transfer and separation of photogenerated electron-hole (e-/h+) pairs, thereby offering prospective application of the photodegradation efficiency for Rhodamine B (RhB) as well as antibacterial applications. With the findings from this study, we could develop efficient and environmentally friendly cellulose/Ag nanoparticles using low-cost, environmentally friendly materials, making them suitable for industrial and technological applications.

Detection of Candida spp. from mastitis cattle milk and study of the antifungal activity of ketoconazole-loaded solid lipid nanoparticles and nanostructured lipid carriers against Candida isolates

Objective: To isolate candida species from cattle milk isolates and investigate the advances of ketoconazole-loadedsolid lipid nanoparticles and ketoconazole-nanostructured lipid carters, and to analyse their properties and comparetheir antifungal performance compared to free ketoconazole.Method: The cross-sectional study was conducted from October 2021 to April 2022 in Waist Governorate of Iraq, andcomprised milk samples obtained from cows infected with mastitis. The study was initiated after obtaining approvalfrom the IRB of the veterinary hospital in the Governorate of Iraq. The samples were cultured on 4% savoured dextroseagar and incubated independently at 37°C before they were subjected to a range of tests. Mastitis was checked witha commercially available cultural and morphological identity card system and through candida chromogenic agar.The data was analyzed by using the programmed SPSS IBM version 20. The chi-square test was used to check theinhibition zones due to the anti-fungal activity of KTZ-LPN and free KTZ. The significance level was kept at p&lt;0.05.Results:Of the 225 samples, 116(51.5%) were obtained from cows infected with mastitis on the basis of a commerciallyavailable cultural and morphological identity card system. Among the 61(52.6%) candida albicans isolated, resistanceto amphotericin B was shown by 60(98%) and to nystatin by 58(95%), while those sensitive to ketoconazole were32(52%) and to fluconazole 31(51%).The ketoconazole nanostructured lipid nanoparticle (KTZ-NLC) preparationsshowed higher antifungal activity than other tested preparations at 0.25ug/ml with a minimum inhibition zone of15±2.4 mm followed by ketoconazole solid lipid nanoparticle (KTZ-SLN) preparations with minimum inhibition zoneof 15±1.3 mm. Free ketoconazole showed the lowest inhibition zone values at the same concentration (0.25ug/ml),which was 11±1.3 mm. Both these lipid nanoparticles formulations, especially ketoconazole nanostructured lipidcarriers (KTZ-NLC), could represent a promising formulation for antifungal application and use.Conclusions: Ketoconazole-nanostructured lipid carter formulations were found to be effective for drug delivery.Keywords: Nanoparticles, Fluconazole, Amphotericin, Ketoconazole, Nystatin, Drug, Milk, Candida, Mastitis.

Static friction, surface roughness, and antibacterial activity of orthodontic brackets coated with silver and silver chitosan nanoparticles

To determine the effect of silver and silver chitosan nanocoatings on monocrystalline ceramic, polycrystalline ceramic, and metallic brackets regarding friction, roughness, and antibacterial effect against Streptococcus mutans. A total of 99 upper right premolar brackets with a 0.022 × 0.025 -inch slot were divided into three groups, each 33 according to coating material; the non-coated group, silver nanoparticles (AgNPs), and silver chitosan nanoparticles (Ag-CsNPs) groups. Each group was equally subdivided into the following three subgroups regarding bracket materials: monocrystalline ceramic, polycrystalline ceramic, and metallic brackets. A universal testing machine determined static friction on a custom-made acrylic jig. Then a profilometer machine was used to collect roughness data, and finally, the anti-cariogenic effect was measured with the disc diffusion technique's "minimum zone of inhibition" against Streptococcus mutans. Two-way ANOVA was used to compare data between groups and subgroups, followed by the Bonferroni test for multiple pair-wise comparisons. The nanocoating effect on ceramic brackets' static friction was non-significant. The AgNPs and Ag-CsNPs coated metallic group revealed a significant increase in static friction-a significant effect of the nanocoating in the surface roughness of monocrystalline and polycrystalline ceramic brackets. A significant favorable effect of AgNPs and Ag-CsNPs against Streptococcus mutans was observed. AgNPs and Ag-CsNPs coats are unsuitable for decreasing friction in metallic brackets or improving roughness in polycrystalline ceramic brackets. Nano coating can improve roughness in monocrystalline ceramic brackets. Coating brackets with AgNPs and Ag-CsNPs has a tremendous antibacterial effect on Streptococcus mutans, a substantial factor in the incidence of dental caries.