Minimum Detection Limit Research Articles

Impact of Transport Mechanism on Binding Kinematics and Sensitivity of FET Biosensors

In this work, the impact of bioanalyte concentration and the dominant transport mechanism of biomolecules on the binding kinematics of the target biomolecules at the sensing surface have been investigated in detail. A comprehensive analysis of the fundamental parameters such as equilibrium response time and equilibrium density of the binding molecules at the sensing surface considering different concentrations of the analytes and their transport mechanisms has been performed. The impact of these realistic artifacts on the current sensitivity of emerging junctionless field effect transistors (JLFETs) and tunnel field effect transistors (TFETs)-based ion-sensitive field effect transistor (ISFET) has been investigated. Moreover, depending on the bioanalyte concentration, design guidelines for the dimensions of the sensing surface have been provided for a minimum limit of detection (LOD). The impact of charge screening and nonspecific binding on current sensitivity has also been discussed in depth. We believe that the guidelines presented in this work will enable the community to conceptualize, understand, and design novel label-free biosensors more rationally considering the practical scenario.

CRISPR/Cas13a-based rapid detection method for porcine deltacoronavirus.

Porcine deltacoronavirus (PDCoV) is a newly discovered porcine intestinal pathogenic coronavirus with a single-stranded positive-sense RNA genome and an envelope. PDCoV infects pigs of different ages and causes acute diarrhea and vomiting in newborn piglets. In severe cases, infection leads to dehydration, exhaustion, and death in sick piglets, entailing great economic losses on pig farms. The clinical symptoms of PDCoV infection are very similar to those of other porcine enteroviruses. Although it is difficult to distinguish these viral infections without testing, monitoring PDCoV is very important because it can spread in populations. The most commonly used methods for the detection of PDCoV is qPCR, which is time-consuming and require skilled personnel and equipment. Many farms cannot meet the conditions required for detection. Therefore, it is necessary to establish a faster and more convenient method for detecting PDCoV. To establish a rapid and convenient detection method for PDCoV by combining RPA (Recombinase Polymerase Isothermal Amplification) with CRISPR/Cas13a. Specific RPA primers and crRNA for PDCoV were designed, and the nucleic acids in the samples were amplified with RPA. Fluorescent CRISPR/Cas13a detection was performed. We evaluated the sensitivity and specificity of the RPA-CRISPR/Cas13a assay using qPCR as the control method. CRISPR/Cas13a-assisted detection was completed within 90 min. The minimum detection limit of PDCoV was 5.7 × 101 copies/μL. A specificity analysis showed that the assay did not cross-react with three other porcine enteroviruses. The RPA-CRISPR/Cas13a method has the advantages of high sensitivity, strong specificity, fast response, and readily accessible results, and can be used for the detection of PDCoV.

Methane gas spectral imaging method based on dual wedge scanning mirrors

With the increase in the number of oil and gas pipelines laid in China, more attention needs to be paid to pipeline maintenance work. At present, the main methods of detecting natural gas leaks in oil and gas transmission stations include manual inspections, opposing natural gas detection equipment, and cloud desktop natural gas detection equipment. Hand held natural gas detection equipment is used for manual inspection, which requires regular manual inspection. However, the response speed is poor and gas leaks cannot be detected in a timely manner. The opposed laser gas detection method can only detect the presence of gas on the beam path. If a larger area of leakage detection is desired, more equipment needs to be installed, resulting in a greatly increase in hardware costs. The existing cloud desktop laser gas detection method controls the deflection of the laser beam through the cloud platform to achieve leak detection at various points in the area to be tested. However, the rotation speed of the cloud platform is slow, and a complete detection cycle takes dozens of minutes, and only the presence of gas can be detected. For accurate leak location, manual on-site survey is also required to further determine the leak location. In order to meet the needs of the real-time monitoring and rapid positioning of oil and gas pipeline leaks, in this work, a fast and accurately controlled dual wedge scanning mirror system is designed, which combines tunable semiconductor laser absorption spectroscopy technology to convert the gas measurement laser beam from point measurement to surface measurement, thereby obtaining the two-dimensional distribution of gas, which is conducive to subsequent analysis and positioning of gas leakage sources. By using the inverse solution iterative optimization algorithm, the angle of the wedge mirror is controlled to obtain an efficient and uniform beam scanning trajectory. The deflection direction and detection position of the laser beam are fused with the corresponding methane concentration information, and a methane concentration data containing position information is constructed. In order to quantitatively verify the measurement accuracy and spatial resolution in the experiment, a standard air bag is used to simulate the methane leakage distribution. The results show that the minimum detection limit of the system can be lower than 5×10<sup>–4</sup> m, and the spatial resolution can be less than 6 cm. At the same time, this method can adjust the scanning step node based on the measurement distance of the oil from gas station, thereby achieving adjustable imaging resolution. This imaging method provides a new idea for accurately positioning and detecting the methane leakage location and amount.

Establishment and application of a gold nanoparticle-based immunochromatographic test strip for the detection of avian leukosis virus P27 antigen in egg white samples.

Avian leukemia is an infectious tumorous disease of chickens caused by subgroup A of the avian leukemia virus (ALV-A), which mainly causes long-term viremia, slow growth, immune suppression, decreased production performance, multi-tissue tumors, and even death. The infection rate of this disease is very high in chicken herds in China, causing huge economic losses to the poultry industry every year. We successfully expressed the specific antigen protein of ALV (P27) through recombinant protein technology and screened a pair of highly sensitive monoclonal antibodies (mAbs) through mouse immunity, cell fusion, and antibody pairing. Based on this pair of antibodies, we established a dual antibody sandwich ELISA and gold nanoparticle immunochromatographic strip (AuNP-ICS) detection method. In addition, the parameters of the dual antibody sandwich ELISA and AuNP-ICS were optimized under different reaction conditions, which resulted in the minimum detection limits of 0.2 ng mL-1 and 1.53 ng ml-1, respectively. Commonly available ELISA and AuNP-ICS products on the market were compared, and we found that our established immune rapid chromatography had higher sensitivity. This established AuNP-ICS had no cross-reactivity with Influenza A (H1N1), Influenza A (H9N2), respiratory syncytial virus (RSV), varicella-zoster virus (VZV), Listeria monocytogenes listeriolysin (LLO), and Staphylococcal enterotoxin SED or SEC. Finally, the established AuNP-ICS was used to analyze 35 egg samples, and the results showed 5 positive samples and 30 negative samples. The AuNP-ICS rapid detection method established by our group had good specificity, high sensitivity, and convenience, and could be applied to the clinical sample detection of ALV-A.

Recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) designed for rapid detection of canine distemper virus.

In the present study, recombinase polymerase amplification (RPA) was combined with the colloidal gold lateral flow dipstick (LFD) method to establish a new, stable, and efficient assay for the detection of canine distemper virus (CDV). We designed a set of specific primers labeled with biotin and a specific probe labeled with dSpacer and C3 spacer, according to the conserved region in the N-terminal gene sequence of CDV. The reaction conditions and systems were then optimized, and the sensitivity and specificity were analyzed for potential clinical application. The results showed that the RPA-LFD assay for CDV detection was successfully established. We also found that the temperature in a closed fist (35°C) is optimal for the RPA reaction. The optimal ratio of primer to probe was 2:1. The minimum detection limit of the RPA-LFD assay was 1 × 101 the median tissue culture infective dose (TCID50)/mL. Using this assay with samples from experimentally infected dogs, CDV was detected in nasal secretions, eye secretions, and blood on the fourth day post infection. In summary, this novel RPA-LFD assay for CDV detection is simple to use, and preliminary findings indicate its high specificity and sensitivity.

Rapid detection of avian leukemia virus using CRISPR/Cas13a based lateral flow dipstick.

Avian leukemia virus (ALV) is one of the main pathogens of poultry tumor diseases, and has caused significant economic losses to the poultry industry since its discovery. Therefore, establishing a rapid detection method is essential to effectively prevent and control the spread of ALV. In this study, specific CRISPR RNA (crRNA) and recombinase-aided amplification (RAA) primers with T7 promoter were designed based on the relatively conserved sequence of avian leukemia virus. When crRNA recognized the target sequence, Cas13a protein was activated to cut the reporting probes, and then the detection results were read by using lateral flow dipstick (LFD). The RAA-CRISPR/Cas13a-LFD reaction system was constructed. The RAA amplification time, Cas13a protein concentration, crRNA concentration and CRISPR reaction time were optimized to evaluate the specificity, sensitivity and reproducibility of the system. Finally, RAA-CRISPR/Cas13a-LFD method was compared with Polymerase chain reaction (PCR)-Agarose electrophoresis method and qPCR method in the detection of clinical samples, and the reliability of RAA-CRISPR/Cas13a-LFD method was evaluated. The results showed that the RAA-CRISPR/Cas13a-LFD method could effectively amplify the target gene at 37°C for 40 min, and the test results could be determined by LFD visual observation. The method had good specificity and no cross-reaction with Marek's disease virus (MDV), Fowl adenovirus (FAdV), Infectious bursal disease virus (IBDV), Newcastle disease virus (NDV), Infectious laryngotracheitis virus (ILTV), and Infectious bronchitis virus (IBV). The minimum detection limit of the method was 100 copies/μL, and it had good repeatability and stability. The coincidence rate of clinical detection reached 97.69% and 99.23%. In summary, this study established a simple, efficient, accurate and visualized ALV detection method, which can be used for the prevention and rapid clinical diagnosis of avian leukosis (AL).

Label-free liquid crystal-based optical detection of norfloxacin using an aptamer recognition probe in soil and lake water.

Norfloxacin (NOX), a broad spectrum fluoroquinolone (FQ) antibiotic, is commonly detected in environmental residues, potentially contributing to biological drug resistance. In this paper, an aptamer recognition probe has been used to develop a label-free liquid crystal-based biosensor for simple and robust optical detection of NOX in aqueous solutions. Stimuli-receptive liquid crystals (LCs) have been employed to report aptamer-target binding events at the LC-aqueous interface. The homeotropic alignment of LCs at the aqueous-LC interface is due to the self-assembly of the cationic surfactant cetyltrimethylammonium bromide (CTAB). In the presence of the negatively charged NOX aptamer, the ordering changes to planar/tilted. On addition of NOX, the aptamer-NOX binding causes redistribution of CTAB at the LC-aqueous interface and the homeotropic orientation is restored. This results in a bright-to-dark optical transition under a polarized optical microscope (POM). This optical transition serves as a visual indicator to mark the presence of NOX. The devised aptasensor demonstrates high specificity with a minimum detection limit of 5 nM (1.596 ppb). Moreover, the application of the developed aptasensor for the detection of NOX in freshwater and soil samples underscores its practical utility in environmental monitoring. This proposed LC-based method offers several advantages over conventional detection techniques for a rapid, feasible and convenient way to detect norfloxacin.

Development of a novel X-ray fluorescence instrument equipped with a noble gas filter.

In X-ray fluorescence (XRF) analysis, which has been used to analyze elements in various samples, it is important to decrease the background (BG) intensity. Generally, BG signals are reduced by inserting metal foils of various types and thicknesses between the X-ray tube and sample as primary X-ray filters. In this study, we developed a unique gas filter-XRF (GF-XRF) instrument to easily reduce the BG effect by changing the pressure of the gas to ensure that the absorption edge of the gas element is slightly lower than the energy of the XRF peak of the target element. The advantage of using a gas is that the gas pressure can be altered easily. To evaluate the performance of this instrument, Ti and Zr were selected as target elements, and Ar and Kr were selected as the filtering gases. When the XRF spectra of the Ti sample were recorded using the Ar gas filter, as the Ar gas pressure increased, the background signal in the energy region of the Ti Kα peak decreased, resulting in an increase in the signal-to-noise ratio (SNR) of that peak. When the Kr gas filter was used, both the SNR and the minimum detection limit of Zr improved. These results demonstrate that the unique GF-XRF instrument is useful for high-sensitivity analyses.

A novel N-CNDs/PAni modified molecular imprinted polymer for ultraselective and sensitive detection of ciprofloxacin in lentic ecosystems: a dual responsive optical sensor.

The research study describes the development of a hybrid nanocomposite called nitro-doped carbon nanodots/polyaniline/molecularly imprinted polymer (N-CNDs/PAni/MIP). This composite is specifically engineered to function as a durable and flexible dual-response sensor to detect and analyze pharmaceutical organic contaminants (POCs). Powder X-Ray diffraction (PXRD), Fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) were employed to perform an exhaustive structural and morphological analysis of N-CNDs/PAni/MIP. N-CNDs/PAni/MIP emitted blue luminescence under ultraviolet irradiation and exhibited typical excitation-dependent emission properties. It can act as fluorescent probe for the detection of CIPRO with high selectivity and sensitivity with an IF value of 4.2. Furthermore, N-CNDs/PAni/MIP exhibited high peroxidase-like catalytic behavior. After adding CIPRO to the N-CNDs/PAni/MIP/TMB/H2O2 system, the blue color of the solution faded due to the reduction of blue ox-TMB to colorless TMB. Based on these two phenomena, with CIPRO as the target analyte, the N-CNDs/PAni/MIP dual sensor showed a minimal detection limit of 70 pM for the fluorescent signaling platform and 3.5 nM for the colorimetric probe with a linear range of 0.038-200 nM. The fluorometric and colorimetric assays based on N-CNDs/PAni/MIP for CIPRO detection were then successfully applied to lentic water as well as to tap water samples, demonstrating the sensitivity and dependability of the instrument. Furthermore, the synthesized PVA (N-CNDs/PAni/MIP) films enable the recognition of CIPRO, and these films have the potential to be integrated into portable sensing devices, providing a practical solution for rapid and on-site detection of CIPRO in various samples.

Green Synthesis and Characterization of Silver Nanoparticles (AgNPs) and L-cysteine-capped AgNPs with Foeniculum vulgare seed extract for Colorimetric Hg2+ Detection

In this study, silver nanoparticles (AgNPs) and L-cysteine-capped AgNPs were synthesized separately using Foeniculum vulgare seed extract as the reducing agent and L-cysteine as the capping agent, which were characterized by ultraviolet–visible spectrophotometer (UV–vis), Fourier Transform Infrared spectrophotometer (FT-IR), X-ray diffractometer (XRD), and transmission electron microscopy (TEM). The utilization of the synthesized nanomaterials as colorimetric sensors for the detection of Hg2+ ions was also investigated. In this context, it was determined that L-cysteine-capped AgNPs exhibited better performance in the colorimetric Hg2+ detection in regards to sensitivity, selectivity, and applicability in real samples. It was observed that the colorimetric detection method was based on the disappearance of the brown color of the nanomaterial-contained colloidal solution and thus the decrease in the LSPR peak intensity. The method of the colorimetric Hg2+ detection with L-cysteine-capped AgNPs showed the good regression coefficient with the minimum detection limit of 0.36 μM in the linear Hg2+ concentration range of 1.0-10 μM, which indicated the competitive results compared to the latest reported colorimetric sensors in the literature. According to the obtained results, it has been concluded that the studied method enables to detection of Hg2+ ions colorimetrically via L-cysteine-capped AgNPs in a sensitive, selective, applicable in real samples, cheap, and easy way.

H2S/Butane Dual Gas Sensing Based on a Hydrothermally Synthesized MXene Ti3C2Tx/NiCo2O4 Nanocomposite.

Real-time sensing of hydrogen sulfide (H2S) at room temperature is important to ensure the safety of humans and the environment. Four kinds of different nanocomposites, such as MXene Ti3C2Tx, Ti3AlC2, WS2, and MoSe2/NiCo2O4, were synthesized using the hydrothermal method in this paper. Initially, the intrinsic properties of the synthesized nanocomposites were studied using different techniques. P-type butane and H2S-sensing behaviors of nanocomposites were performed and analyzed deeply. Four sensor sheets were fabricated using a spin-coating method. The gas sensor was distinctly part of the chemiresistor class. The MXene Ti3C2Tx/NiCo2O4-based gas sensor detected the highest response (16) toward 10 ppm H2S at room temperature. In comparison, the sensor detected the highest response (9.8) toward 4000 ppm butane at 90 °C compared with the other three fabricated sensors (Ti3AlC2, WS2, and MoSe2/NiCo2O4). The MXene Ti3C2Tx/NiCo2O4 sensor showed excellent responses, minimum limits of detection (0.1 ppm H2S and 5 ppm butane), long-term stability, and good reproducibility compared with the other fabricated sensors. The highest sensing properties toward H2S and butane were accredited to p-p heterojunctions, higher BET surface areas, increased oxygen species, etc. These simply synthesized nanocomposites and fabricated sensors present a novel method for tracing H2S and butane at the lowest concentration to prevent different gas-exposure-related diseases.

Photoacoustic Heterodyne CO Sensor for Rapid Detection of CO Impurities in Hydrogen.

Hydrogen (H2) fuel cells have been developed as an environmentally benign, low-carbon, and efficient energy option in the current period of promoting low-carbon activities, which offer a compelling means to reduce carbon emissions. However, the presence of carbon monoxide (CO) impurities in H2 may potentially damage the fuel cell's anode. As a result, monitoring of the CO levels in fuel cells has become a significant area of research. In this paper, a novel photoacoustic sensor is developed based on photoacoustic heterodyne technology. The sensor combines a 4.61 μm mid-infrared quantum cascade laser with a low-noise differential photoacoustic cell. This combination enables fast, real-time online detection of CO impurity concentrations in H2. Notably, the sensor requires no wavelength locking to monitor CO online in real-time and produces a single effective signal with a period of only 15 ms. Furthermore, the sensor's performance was thoroughly evaluated in terms of detection sensitivity, linearity, and long-term stability. The minimum detection limit of 11 ppb was obtained at an optimal time constant of 1 s.

Multigas nonresonant photoacoustic spectroscopy sensor based on a broadband radiation source

AbstractMultigas detection is in demand for a wide range of environmental and industrial applications. In this work, a sensitive and selective multigas photoacoustic spectroscopy (MG‐PAS) sensor was developed by employing a nonresonant photoacoustic spectroscopy technique combining a broadband radiation source. Six gases including CO, CO2, CH4, C2H2, C2H4, and C2H6 were selected as target measurement molecules. Selectivity of the MG‐PAS was realized by using six mid‐infrared narrow‐band filters which integrated in a homemade filter wheel. A minimum concentration detection limits of 0.2 ppm for CO, 0.05 ppm for CO2, 0.12 ppm for CH4, 0.1 ppm for C2H2, 0.81 ppm for C2H4, and 0.05 ppm for C2H6 were obtained, respectively, with a 1‐s integration time. Measurements of six gas mixtures showed that the average measurement error of the MG‐PAS sensor was within 2%.

Risk assessment from radon in domestic water for the Greek population.

This study focused on assessing the risk from the exposure to radon contained in domestic water for a significant part (~20%) of the Greek population. Also, the variation of radon in domestic water was monitored from 2017 to 2023 in certain villages that showed relatively high radon levels and relied on boreholes for their water supply. The radon in domestic water activity concentrations measured in the investigated Greek places ranged from lower than the minimum detection limit (2BqL-1) levels up to 187BqL-1 with an average value of 9.1BqL-1. Overall, higher radon in domestic water activity concentrations were observed in places supplied from boreholes located inside granitic and metamorphic rock areas. Only one out of the 487 examined places, which accounts for 0.015% of the examined Greek population, showed an average radon-in-water activity concentration higher than the parametric value of 100BqL-1 adopted by Greece following the EURATOM Directive (2013/51/EURATOM). Therefore, radon-in-water does not pose a health concern (risk) for the investigated Greek population. The total (inhalation and ingestion) annual effective doses to adults, corresponding to the measured radon-in-water activity concentrations, ranged from nearly 0 to 1.20mSv y-1 with an average value of 0.059mSv y-1, while for children, they ranged from almost 0 to 1.26mSv y-1 with an average value of 0.061mSv y-1. Regarding the variation of radon in domestic water monitoring, places supplied with water from one borehole showed no significant fluctuations from their average radon-in-water activity concentration, with standard deviations of ~20% at a coverage factor of k=1. Even though some places supplied from three to four boreholes showed no significant fluctuations (standard deviation <= 30% at k=1) from their average radon level, special attention is needed for places supplied from many boreholes when one measurement over the year is to be performed for the annual effective dose assessment. This is because the prevailing during-year borehole combination may not exist on the measurement day, resulting in an underestimated or overestimated dose assessment. Radon removal from domestic water supplies in the Arnea village (due to elevated radon-in-water activity concentrations) did not affect the inhalation risk for the residents of an examined house in Arnea. However, radon removal from the water supply was essential to reduce the ingestion risk for the house occupants. There is a possibility of radiation overexposure (>20mSv y-1) for the workers in a thermal spa on Ikaria Island, and further investigation needs to be conducted with extended measurement periods.

Multifunction Applications of Filtering Dielectric Resonator Antenna Based on Liquid Crystal.

In this paper, a new type of multifunctional device is realized by designing a filtering dielectric resonator antenna (FDRA) with liquid crystal (LC). The LC is encapsulated by glass plates and placed between the feeding network and the ground. Firstly, the resonance frequencies of the hairpin bandpass filter (|S11| is less than -10 dB) move simultaneously when the dielectric constant of LC changes at different temperatures. Then, the hairpin bandpass filter is extended to an FDRA, and the influence of the dielectric constant of LC on the antenna performance parameters is realized to the function of the temperature sensor. The results show that the dielectric constant of LC has an approximately linear relationship with the resonance frequencies of the FDRA. Simultaneously, the axial ratio, gain, antenna efficiency, E-field distribution, and pattern of the FDRA have changed significantly. Furthermore, the FDRA mainly works in the frequency range of 4.65~5.53 GHz, which has good antenna performance and filtering characteristics. Taking resonance frequency fx as an example, its sensitivity, maximum FOM, minimum detection limit, and minimum resolution are determined to be 95 GHz/RIU, 0.5, 0.1, and 9.68, respectively. The multifunctional device provides a novel approach and solution for the transmission of antenna signals and temperature measurements.

Precision measurements of U3O8 and Ra(eU3O8) in uraniferous samples by gamma ray spectrometry using two HPGe coaxial P-type detectors (GCD30180 & GC5019 models)-a comparative study.

The precision measurements of U3O8 and Ra(eU3O8) concentrations were evaluated in 23 geological rock samples by high resolution gamma ray spectrometry (HPGe) using two P-type HPGe detectors. The use of Detector 2 (DET2) (GC5019) has increased the sensitivity by a factor of two for both the measurements in comparison to Detector 1 (DET1) (GCD30180). The Minimum Detection Limit (MDL) and Limit of quantification (LOQ) for U3O8 in DET2 has increased significantly because of negligible background counts in 1.001MeV ϒ energy peak (0.838% gamma yield). The MDL for Ra(eU3O8) measured by 0.609MeV and 1.760MeV has not improved significantly due to the high background counts in these energies whereas LOQ has improved appreciably. DET 2 has given higher level of confidence in reporting the analytical results with acceptable repeatability due to the decrease in uncertainty of the measurements.

Trace gas sensor based on a multi-pass-retro-reflection-enhanced differential Helmholtz photoacoustic cell and a power amplified diode laser.

A high-sensitive photoacoustic spectroscopy (PAS) sensor, which is based on a multi-pass-retro-reflection-enhanced differential Helmholtz photoacoustic cell (DHPAC) and a high power diode laser amplified by erbium-doped fiber amplifier (EDFA), is presented in this work for the first time. In order to improve the interaction length between the light and target gas, the incident light was reflected four times through a multi-pass-retro-reflection-cell constructed by two right-angle prisms. A 1.53 µm distributed feedback (DFB) diode laser was selected to excite photoacoustic signal. Moreover, its power was amplified by an EDFA to 1000 mW to improve the amplitude of photoacoustic signal. Acetylene (C2H2) was chosen as the target analysis to verify the reported sensor performance. Compared to double channel without multiple reflections, the 2f signal of double channel with four reflections was improved by 3.71 times. In addition, when the output optical power of EDFA was 1000 mW, the 2f signal has a 70.57-fold improvement compared with the multi-pass-retro-reflection-cell without EDFA. An Allan deviation analysis was carried out to evaluate the long-term stability of such PAS sensor. When the averaging time was 400 s, the minimum detection limit (MDL) of such PAS sensor was 14 ppb.

A compact portable photoacoustic sensor for sub-ppm level SO2 detection with a DUV-LED and a non-resonant photoacoustic cell

A photoacoustic module based on a novel 273 nm deep-ultraviolet light-emitting diode (DUV-LED) was developed for sulfur dioxide (SO2) detection. The DUV-LED with an emission angle of 60° emitted a power of 10 mW. A high-sensitivity non-resonant photoacoustic cell with a gas chamber volume of only 0.39 mL was introduced to reduce the thermal noise generated by the contact of scattered DUV light with the photoacoustic cell. Assembly of the DUV-LED with the non-resonant photoacoustic cell resulted in a robust and portable photoacoustic module without any moving parts. The minimum detection limit of 725 ppb was achieved with 1s integration time at room temperature and atmospheric pressure. Furthermore, the photoacoustic module performance was evaluated in terms of DUV modulation frequency, linearity and the long-term stability.

Highly luminescent heteroatom codoped carbon dots for multitudinous applications

Carbonaceous nanomaterials have shown great potential in the analytical and environmental domains due to their natural intriguing properties. Rich in quantum yield and reliable performances are necessary for carbon dots to be effectively used in the real-world applications. Carbon dots (CDs) exhibits high blue-emission with a quantum yield (ϕ) of 11% were synthesized through a single-step superficial pyrolysis technique. In this work, the derived carbon dots were employed as reducing and stabilizing agents in a unique one-step approach for the synthesis of AgNPs. The fluorescence of CDs was found to be quenched in the presence of dopamine with greater sensitivity, the minimum limit of detection was determined as 0.35 μM and possible mechanism could be the inner filter effect along with photoinduced electron transfer. In addition to this, the outstanding optical properties of CDs enable them to use as invisible ink in loading secret information and provide enhanced counterfeit protection. The solid-state quenching is successfully prevented when the CDs were dispersed in a polymer matrix for solid-state films preparation. The CDs-polymer composite is packed on top of a 365 nm UV LED chip, which emits blue light, to fabricate blue LED. Hence, we suggest that the synthesized CDs may serve as a future trend setter by as a single molecular probe for multifarious applications such as optoelectronics and forensic fields. Previously, we utilized these CDs for fingerprint detection and now apply them for new emerging applications.

Performance and sensitivity of column-wise and pixel-wise methane retrievals for imaging spectrometers

Abstract. Strong methane point source emissions generate large atmospheric concentrations that can be detected and quantified with infrared remote sensing and retrieval algorithms. Two standard and widely used retrieval algorithms for one class of observing platform, imaging spectrometers, include pixel-wise and column-wise approaches. In this study, we assess the performance of both approaches using the airborne imaging spectrometer (Global Airborne Observatory) observations of two extensive controlled-release experiments. We find that the column-wise retrieval algorithm is sensitive to the flight line length and can have a systematic low bias with short flight lines, which is not present in the pixel-wise retrieval algorithm. However, the pixel-wise retrieval is very computationally expensive, and the column-wise retrieval algorithms can produce good results when the flight line length is sufficiently long. Lastly, this study examines the methane plume detection performance of the Global Airborne Observatory with a column-wise retrieval algorithm and finds minimum detection limits of between 9 of 10 kg h−1 and 90 % probability of detection between 10 and 45 kg h−1. These results present a framework of rules for guiding proper concentration retrieval selection given conditions at the time of observation in order to ensure robust detection and quantification.