Minimal Damage Research Articles

Development and performance evaluation of an electric cabbage harvester with a precise cabbage pushing mechanism

The manual method of harvesting cabbages is common in many Asian countries, which creates a lot of biomechanical problems among farmers. The existing commercial cabbage harvesters being bigger in size are not suitable for small landholdings. To address this, a three-wheeled walk-behind type electric cabbage harvester with a precise cabbage pushing mechanism was developed. It comprised a cutting unit, pushing unit, conveying unit, propelling unit, storage bin and handle. Based on the results obtained from laboratory tests, the cutting speed, forward speed and cutting position of cabbage stem from cabbage head was chosen as 590 rpm, 0.25 m/s and 0.1 cm. For pushing the cut cabbages precisely, a YOLOv8 custom cabbage detection model (mean average precision of 0.938) was developed and deployed in Nvidia Jetson Nano that could detect the cabbages and actuated a pusher via Arduino microcontroller and motor driver to push the cut cabbages to the conveyor belt. In the power supply unit of the harvester, 48 V 12 Ah, 12 V 7 Ah, and 24 V 18 Ah batteries were used for powering the motors for cutting, pushing, and propelling and conveying, respectively. The harvester was tested in a polyhouse for three Indian cabbage varieties. The maximum instantaneous power consumption (IPC) was measured as 948.53 W. Hand vibration and noise at operators’ ear level were 2.098 m/s2 and 78 dB(A), respectively, which confirmed its safety from an ergonomic perspective. The harvester achieved a maximum cutting efficiency of 77.5 % and a minimum damage rate of 7.5 % during polyhouse testing.

Sustainability in small and medium enterprises: A circular economy approach using cloud computing

AbstractSustainable production and consumption are the need of the hour. Adopting the Circular Economy (CE) approach fosters sustainable business practices. Cloud computing is a new way of storage and application deployment. Cloud computing enables sustainable business processes, which in turn contributes towards the effective implementation of the Circular Approach to Business Practices (CABP). The current study aims to understand the potential of cloud computing to foster circular economy practices in Small and Medium Enterprises (SMEs). We conducted a Systematic review of 186 scholarly articles published in Web of Science (WOS), selected using the PRISMA framework. We conducted scientometric analysis on sources, authors and country level and thematic analysis to pinpoint further research areas. This was followed by a detailed literature review of the articles selected to study the concept and current practices of circular economy and an evaluation of its implementation in emerging economies. The paper proposes an integrated framework grounded in two approaches, that is, the Technology, Organization and Environment (TOE) framework and institutional isomorphism as determinants of cloud adoption and CE implementation. The proposed model explores the impact of institutional forces on the various parameters of TOE, thereby culminating in the adoption of Cloud Computing in SMEs. The study would encourage leaders and managers of SMEs to adopt cloud computing for competitive advantage and achieve a circular economy approach together. The adoption of cloud‐based applications has a great potential to foster industrial and economic progress with minimum damage to the ecosystem, furthering sustainability and achieving Sustainable Development Goals (SDGs).

Minimally Invasive Delivery of Percutaneous Ablation Agent via Magnetic Colloidal Hydrogel Injection for Treatment of Hepatocellular Carcinoma.

Percutaneous thermotherapy, a minimally invasive operational procedure, is employed in the ablation of deep tumor lesions by means of target-delivering heat. Conventional thermal ablation methods, such as radiofrequency or microwave ablation, to a certain extent, are subjected to extended ablation time as well as biosafety risks of unwanted overheating. Given its effectiveness and safety, percutaneous thermotherapy gains a fresh perspective, thanks to magnetic hyperthermia. In this respect, an injectable- and magnetic-hydrogel-construct-based thermal ablation agent is likely to be a candidate for the aforementioned clinical translation. Adopting a simple and environment-friendly strategy, a magnetic colloidal hydrogel injection is introduced by a binary system comprising super-paramagnetic Fe3O4 nanoparticles and gelatin nanoparticles. The colloidal hydrogel constructs, unlike conventional bulk hydrogel, can be easily extruded through a percutaneous needle and then self-heal in a reversible manner owing to the unique electrostatic cross-linking. The introduction of magnetic building blocks is exhibited with a rapid magnetothermal response to an alternating magnetic field. Such hydrogel injection is capable of generating heat without limitation of deep penetration. The materials achieve outstanding therapeutic results in mouse and rabbit models. These findings constitute a new class of locoregional interventional thermal therapies with minimal collateral damages.

Positional Effects of Bottle-Baited Traps in Reducing Infestation Level of Coffee Berry Borer Hypothenemus Hampei Ferrari in Kilimanjaro Region, Tanzania

Coffee berry borer (CBB) is among the key insect pests of coffee worldwide. The use of bottle-baited traps has been in practice in several coffee-growing areas including Tanzania. However, there is limited information about the influence of height and spacing of commonly used bottle-baited traps in managing CBB in coffee-growing areas in the country. Therefore, the objective of this research was to evaluate the effect of height where traps were placed (0.6, 1.2, and 1.6 m) on the reduction of infestation level of coffee berry borers at different developmental stages of coffee fruit (green and red fruit) under field conditions. The experiment followed a completely randomized block design with a factorial arrangement and four replications, three (lower, middle, and upper) levels of height and spacing were placed for 7 months. The number of captured CBB and damaged berries percentage was evaluated. The data were analyzed by R Software (2021) through an analysis of variance and means were separated by Turkey’s (0.05). A significant minimum berries damage (0.26%) as an implication of the lowest CBB infestation level was shown at the height of 0.6 m (for all stages of berries). On the other hand, at the red berries stage, the lowest damage (11.12%) was observed at the height of 1.6 m. Generally, this study deduced that the lower the height from which the traps are placed, the lower the infestation level of CBB hence reducing crop damage by the pest.

A vasculature based nanocryosurgical model for tumor destruction

The vascular model built on porous media approach is a more realistic representation of biological tissue. A vasculature system based axisymmetric cryosurgical model is proposed here for cryosurgery of lung tumor based on the freezing of the vascular region of the biological tissue. A two-temperature model is developed based on the porous medium approach considering the thermal non-equilibrium between the tissue and the blood region. Bioheat transfer phenomenon between tissue and blood involves interfacial heat transfer, perfusion heat transfer and metabolic heat generation in tissue cylinder. To improve the cryothermal penetration inside the tumor tissue, high conductive nanoparticles are injected into it which increases the local freezing rate of tumor region with minimum damage to the surrounding healthy tissue. The blood phase change in the vascular network is modelled through a source based implicit enthalpy method. The proposed model is utilized to simulate cryosurgery for lung lesion. It is observed that the solidification time for 99.9% tumor using Pennes model and Vascular model (ε=0.01) are 414 s and 512 s respectively, which shows that Pennes model overpredicts the freezing of tumor during cryosurgery. The freezing necrosis is found to reduce by 13.95% at 0.05 (volume fraction) nanoparticle concentration.

Damage of Melanagromyza obtusa (Malloch) and Management Potential of Newer Insecticides in Pigeon Pea

Background: The pod fly, Melanagromyza obtusa (Malloch) is one of the major biotic constraints in increasing production and productivity of pigeonpea crop throughout the country and it causes up to 100 per cent losses in field condition. Injudicious use of pesticides against this destructive pest, further ignites the problem of resistance, resurgence and environmental and ecological imbalances. Methods: Experiments were conducted at Banda University of Agricultural and Technology, Banda during kharif, 2020 and 2021. To estimate the bio-efficacy of insecticides for the management of M. obtusa in pigeonpea the experiment was laid out in randomized block design (RBD) with eight treatments and three replications. Pod and grain damage were assessed and C:B ratio was calculated. Result: First appearance of pod fly was noticed in third standard week. Throughout cropping period highest pod damage was recorded in eighth standard week and lowest pod damage was recorded in twelfth standard week. Emamectin benzoate 5 SG @ 11g a.i./ha + Dimethoate 30 EC @ 300 g a.i./ha was the best treatment as it exhibited minimum pod damage and highest yield.

High Precision Nail‐Penetration Setup for the Controlled Thermal Runaway Initiation of Lithium‐Ion Cells at Very Low Temperatures

A high precision nail‐penetration (NP) tool for characterizing the mechanically induced thermal‐runaway (TR) of lithium‐ion battery (LIB) cells in a defined range of temperatures down to −140 °C was developed. To understand the cell specific behavior at low temperatures aiming at the determination of safe handling conditions, different scenarios are analyzed. First, accuracy tests of the NP‐tool regarding motion and penetration depth are conducted with cylindrical cells at different temperatures. Thus, postmortem computer tomographic (CT) images are compared to the data measured with the newly integrated 3‐axis force sensor which is further combined with a high‐resolution position sensor. The herein developed setup allows evaluation of the NP‐metrics at an accuracy of ±1 pierced electrode layer without CT‐scans. Further NP examinations at 20 °C of fully charged cylindrical lithium nickel manganese cobalt oxide cells reveal a reproducible minimum damage as a reliable TR‐trigger. Moreover, NP‐tests at low temperature disclose a relation of the short circuit conductivity and TR‐reactions during subsequent rethermalization to room temperature. Finally, the implementation of a novel fixture for a controlled very fast cooling of LIB‐cells during critical damage opens the way to investigate the individual steps during a TR and, thus, to gain important information of the specific TR‐mechanism of different LIB‐cells.

Investigation of Dynamic Behavior of Piled Pier Structure

Sea transport is preferred because it is safe and economical in countries in the regions that dominate world trade. Approximately 86% of the developing and increasing trade volume is provided by sea transportation. Ports, which are an important pillar of international trade, take an active place in the transportation network for these reasons. In our country, an increase has been observed in the studies on the design of coastal port structures in the last 15 years. In the design of coastal structures, technical approaches and durability are at the forefront, while recently the continuity of the performance of the structures has been taken as a basis. They are exposed to static and dynamic loads throughout their service life. In order to encounter minimum damage due to earthquake effects, an analysis that reflects the behavior under static and dynamic loads and the parameters must be determined correctly. For this purpose, parameter changes were investigated to examine the dynamic behavior of a scaffolding structure. Piles diameters of 600, 800 and 1000 mm and placement spacing of 4D and 8D were considered variable. Analyzes were carried out with Sap 2000, a finite element program. The capacity of the building was determined with pushover analysis and its dynamic performance was evaluated with time history analysis.

Differentiation of blue gel pens using visible spectroscopy coupled with chemometric techniques

Ink analysis is one of the most important and keydimension of forensic document examination (FDE). It is performed to compare, identify, characterize and discriminate different kinds of inks present on questioned document. It becomes key evidence in forensic document examination. In the present work, thirty-eight blue gel pens are discriminated using visible spectroscopy coupled with chemometric techniques. The recorded spectra were visually compared followed by chemometric techniques of principal component analysis (PCA) and linear discriminant analysis (LDA). Discriminating power for visual comparison, PCA and LDA are 58.18%, 100% and 100% respectively. Developed PCA model is validated and 100% accuracy, precision, sensitivity and specificity are achieved. Blue gel pen inks were 100% correctly differentiated on the basis of their brands using PCA followed by LDA. The utilized approach is fast and provides reproducible results. The results of present work demonstrate that potential utility of conventional and cost-effective visible spectroscopy can be improved by coupling it with these chemometric techniques. Major advantage of present research is the minimum damage caused to document. This study establishes a method which provides proof of concept discrimination of the gel pen samples. The methodology adopted in present work can be applied in the alteration cases in examination of suspected documents.

Abstract 3389: Intraductal ablative procedure for local preventive intervention in rat models of breast cancer

Abstract There are limited options for primary prevention of breast carcinoma (BC). Prophylactic mastectomy and systemic hormonal therapy are only recommended for high-risk individuals. Prophylactic mastectomy is a drastic surgery that removes the entire breast, including the epithelial cell-lined ductal trees where BC arises but also uninvolved stroma. Even with a 90% risk reduction, many women are deterred from prophylactic mastectomy due to its associated risk of infection, lymphedema, painful recovery time, and mental health toll. Hormonal therapies, tamoxifen and raloxifene, reduce BC risk by upwards of 50% but also increase the risk of uterine cancer and stroke. Thus, new interventions to reduce the risk of BC and minimize deterrents are necessary. Intraductal (ID) injection is a minimally invasive procedure that avoids systemic toxicity by cannulating a needle directly into the ductal tree opening to directly administer a solution to the epithelial cells. We previously reported that ID infusion of 70% ethanol (EtOH) as a cell-killing solution prevents tumor formation in an aggressive genetically engineered mouse model of BC with minimum collateral tissue damage. Here, we investigated the therapeutic efficacy of EtOH-based ablative solutions in a Methyl-N-Nitrosourea (MNU) chemically induced rat model of BC to assess scalability of this procedure towards clinical trials. Two weeks after tumor induction, rats received ID infusions of a solution containing 70% EtOH to ablate epithelial cells, ethyl cellulose (EC) to minimize collateral tissue damage, and/or Tantalum Oxide (TaOx) to visualize infused ductal trees via micro-CT imaging. EtOH-infused glands had the longest tumor latency (273 days vs 97 days in control group, p < 0.0001). No statistically significant differences were noted in epithelial ablation or collateral tissue damage between treatment groups receiving ID injections of 70% EtOH with varying concentrations of EC, but there was a trend to lower collateral damage with EC-containing solutions. Local clearance of TaOx in infused mammary glands enabled post-procedure MR imaging surveillance after 15 d of ID procedure. This preclinical study demonstrates that ID injection of a refined solution containing 70% EtOH and TaOx is an efficacious and scalable procedure for primary prevention of BC. Citation Format: Erin Zaluzec, Mohamed Ashry, Elizabeth G. Phelps, Katarzyna Kempinska, Elizabeth Kenyon, Legend Kenny, Max Volk, Katherine Powell, Jeremy ML Hix, Christiane Mallett, Matti Kiupel, Erik M. Shapiro, Lorenzo F. Sempere. Intraductal ablative procedure for local preventive intervention in rat models of breast cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 3389.

Rapid Visual Screening Feature Importance for Seismic Vulnerability Ranking via Machine Learning and SHAP Values

Structures inevitably suffer damage after an earthquake, with severity ranging from minimal damage of nonstructural elements to partial or even total collapse, possibly with loss of human lives. Thus, it is essential for engineers to understand the crucial factors that drive a structure towards suffering higher degrees of damage in order for preventative measures to be taken. In the present study, we focus on three well-known damage thresholds: the Collapse Limit State, Ultimate Limit State, and Serviceability Limit State. We analyze the features obtained via Rapid Visual Screening to determine whether or not a given structure crosses these thresholds. To this end, we use machine learning to perform binary classification for each damage threshold, and use explainability to quantify the effect of each parameter via SHAP values (SHapley Additive exPlanations). The quantitative results that we obtain demonstrate the potential applicability of ML methods for recalibrating the computation of structural vulnerability indices using data from recent earthquakes.

Effect of enzymatic hydrolysis of arabinoxylan on the quality of frozen dough during the subfreezing process.

The objective of this investigation was to examine the impact of enzymatic hydrolysis of arabinoxylan (AX) on frozen dough quality under subfreezing conditions. The dough was subjected to freezing at -40 °C for 2 h and then stored at -9, -12, and -18 °C for 15 days. The water loss, freezable water content, water migration, and microstructure of the dough were measured. The dough containing 0.8% cellulase enzymatically hydrolyzed AX (CAX) required the shortest duration when traversing the maximum ice-crystal formation zone (6.5 min). The dough with xylanase enzymatically hydrolyzed AX (XAX) demonstrated a faster freezing rate than the dough with CAX. The inclusion of both XAX and CAX in the dough resulted in the lowest freezable water loss and reduced freezable water content and free-water content levels, whereas the inclusion of xylanase-cellulase combined with enzymatically hydrolyzed AX resulted in higher free-water content levels. The textural properties of the subfreezing temperature dough were not significantly different from the dough stored at -18 °C and sometimes even approached or surpassed the quality observed in the control group rather than the dough stored at -18 °C. In addition, the gluten network structure remains well preserved in XAX- and CAX-containing doughs with minimal starch damage. The enzymatic hydrolysis of AX from wheat bran can be used as a useful additive to improve the quality of frozen dough. © 2024 Society of Chemical Industry.

Support-Based Transfer and Contacting of Individual Nanomaterials for In Situ Nanoscale Investigations.

Although in situ transmission electron microscopy (TEM) of nanomaterials has been gaining importance in recent years, difficulties in sample preparation have limited the number of studies on electrical properties. Here, a support-based preparation method of individual 1D and 2D materials is presented, which yields a reproducible sample transfer for electrical investigation by in situ TEM. A mechanically rigid support grid facilitates the transfer and contacting to in situ chips by focused ion beam with minimum damage and contamination. The transfer quality is assessed by exemplary specimens of different nanomaterials, including a monolayer of WS2 . Possible studies concern the interplay between structural properties and electrical characteristics on the individual nanomaterial level as well as failure analysis under electrical current or studies of electromigration, Joule heating, and related effects. The TEM measurements can be enriched by additional correlative microscopy and spectroscopy carried out on the identical object with techniques that allow a characterization with a spatial resolution in the range of a few microns. Although developed for in situ TEM, the present transfer method is also applicable to transferring nanomaterials to similar chips for performing further studies or even for using them in potential electrical/optoelectronic/sensingdevices.

Pediatric acute urinary retention due to impacted urethral meatal calculus: a rare case report

Urethral calculus is rare in pediatrics, especially with acute urinary retention. The diagnosis of urethral calculus is made by proper history taking, physical examination, and imaging studies. In this case, the diagnosis was made mainly by anamnesis, inspection, and palpation of the calculus in the external urethral meatus. Invasive and less invasive procedures such as open surgery, meatotomy, or laser lithotripsy procedure, can be a management of urethral calculus. In this case, simple extraction was performed without meatotomy or other invasive procedures. A 5-year-old, boy was reported to come to the emergency department because of severe pain in his penis and acute urinary retention. The patient had a history of dysuria, a habit of delaying urination, and low fluid intake. On physical examination, it was found a distention in the suprapubic area and a green-white solid mass at the external urethral meatus. A 10-millimeter calculus was successfully extracted from the external urethral meatus with simple extraction procedures under local anesthesia. This case report demonstrated a rare case of pediatric acute urinary retention secondary to impacted urethral meatal calculus in an emergency setting of a low-level hospital. Immediate and proper management was required due to the patient's condition and considering the lack of facilities. The extraction of calculus with minimal urethral damage was the recommendation.

Foreign bodies of the stomach with the introduction into the mucous membrane of a 9-year-old child

A description of the clinical observation of a 9-year-old child with foreign bodies of the stomach (2 magnetic balls) and their introduction into the mucous membrane is presented. Timely endoscopic and X-ray examinations contributed to the establishment of the exact localization of foreign objects, and videoesophagogastroscopy using a magnetic extractor made it possible to extract magnets from the mucous membrane with minimal damage to the stomach tissues and prevent the development of complications in this patient.

Flexible Polymer Electrodes for Stable Prosthetic Visual Perception in Mice.

Brain interfaces that can stimulate neurons, cause minimal damage, and work for a long time will be central for future neuroprosthetics. Here, the long-term performance of highly flexible, thin polyimide shanks with several small (<15µm) electrodes during electrical microstimulation of the visual cortex, is reported. The electrodes exhibit a remarkable stability when several billions of electrical pulses are applied in vitro. When the devices are implanted in the primary visual cortex (area V1) of mice and the animals are trained to detect electrical microstimulation, it is found that the perceptual thresholds are 2-20 microamperes (µA), which is far below the maximal currents that the electrodes can withstand. The long-term functionality of the devices in vivo is excellent, with stable performance for up to more than a year and little damage to the brain tissue. These results demonstrate the potential of thin floating electrodes for the long-term restoration of lost sensory functions.

Cryotherapy-Induced Iatrogenic Frostbite on Distal Digit: Case Report and Review of Management Strategies.

Cryotherapy is a widely utilized topical treatment for various dermatological conditions. While generally safe and effective, it carries potential risks, such as frostbite injury. Frostbite, a vascular condition causing skin and tissue freezing, is usually associated with prolonged exposure to freezing environments. Certain populations, like military personnel navigating frigid terrains, face an increased risk of frostbite and other cold-weather injuries. In this case, we present an unusual instance of iatrogenic frostbite in a 23-year-old active duty United States Navy Sailor. The patient required a diverse range of treatment modalities, including pain management, sildenafil, nitroglycerine paste, and hyperbaric oxygen therapy. The implemented treatment regimen resulted in significant clinical improvements with minimal long-term structural damage. This case underscores the clinical value of establishing clear management guidelines for cryotherapy administration in cutaneous conditions. Such guidelines are essential to ensure optimal dosing, minimize adverse sequelae, and improve overall prognosis.

Optimization of drilling parameters on delamination and burr formation in drilling of neat CFRP and hybrid CFRP nano-composites

Carbon fibre-reinforced polymer (CFRP) composites have exceptional mechanical advantages such as high specific strength and stiffness, lightweight, and high damping capacity, making them very attractive for aircraft, aerospace, automotive, marine, and sporting applications. However, various defects such as delamination, burr formation, and surface roughness are observed during the drilling of CFRP composites, which are influenced by various drilling process parameters. In this work, the drilling quality of uni-directional CFRP composites. and the hybrid Al2O3 and hybrid SiC nano-composites are investigated experimentally using different types of drills such as step drill, core drill, and twist drill, as there is a limited study done on the comparative analysis of the impact of the above drills on the delamination factor and burr area on the above CFRP and hybrid nano-composites. The design of the experiment table was developed using response surface methodology (RSM) for input process parameters of spindle speed, feed, drill diameter, and drill type. The output surface characteristics (delamination factor and burr area) of the hole were measured quantitatively using the stereo zoom optical microscope. The main effect plots, contour plots, and analysis of variance (ANOVA) were used to examine the effect of spindle speed, feed, drill diameter, and drill type on exit delamination and burr formation. The analysis of main effect plots, contour plots, and analysis of variance showcased the optimum process parameters, such as a high spindle speed of 5500 rpm, low feed of 0.01 mm/rev, and drill diameter of 4 mm. The step drill demonstrated the least damage mechanism among drill geometries, followed by the twist and core drills. The minimum drilling damage was observed for the Al2O3 hybrid nano-composite compared to the neat CFRP composites.

Designing Gold Nanoparticles for Precise Glioma Treatment: Challenges and Alternatives.

Glioblastoma multiforme (GBM) is a glioma and the most aggressive type of brain tumor with a dismal average survival time, despite the standard of care. One promising alternative therapy is boron neutron capture therapy (BNCT), which is a noninvasive therapy for treating locally invasive malignant tumors, such as glioma. BNCT involves boron-10 isotope capturing neutrons to form boron-11, which then releases radiation directly into tumor cells with minimal damage to healthy tissues. This therapy lacks clinically approved targeted blood-brain-barrier-permeating delivery vehicles for the central nervous system (CNS) entry of therapeutic boron-10. Gold nanoparticles (GNPs) are selective and effective drug-delivery vehicles because of their desirable properties, facile synthesis, and biocompatibility. This review discusses biomedical/therapeutic applications of GNPs as a drug delivery vehicle, with an emphasis on their potential for carrying therapeutic drugs, imaging agents, and GBM-targeting antibodies/peptides for treating glioma. The constraints of GNP therapeutic efficacy and biosafety are discussed.

Nanoparticle-Terpene Fusion: A Game-Changer in Combating Primary Amoebic Meningoencephalitis Caused by Naegleria fowleri.

Pathogenic Naegleria fowleri (N. fowleri) are opportunistic free-living amoebae and are the causative agents of a very rare but severe brain infection called primary amoebic meningoencephalitis (PAM). The fatality rate of PAM in reported cases is more than 95%. Most of the drugs used againstN. fowleri infections are repurposed drugs. Therefore, a large number of compounds have been tested againstN. fowleri in vitro, but most of the tested compounds showed high toxicity and an inability to cross the blood-brain barrier. Andrographolide, forskolin, and borneol are important natural compounds that have shown various valuable biological properties. In the present study, the nanoconjugates (AND-AgNPs, BOR-AgNPs, and FOR-AgNPs) of these compounds were synthesized and assessed against both stages (trophozoite and cyst) ofN. fowleri for their antiamoebic and cysticidal potential in vitro. In addition, cytotoxicity and host cell pathogenicity were also evaluated in vitro. FOR-AgNPs were the most potent nanoconjugate and showed potent antiamoebic activity againstN. fowleriwith an IC50 of 26.35 μM. Nanoconjugates FOR-AgNPs, BOR-AgNPs, and AND-AgNPs also significantly inhibit the viability of N. fowleri cysts. Cytotoxicity assessment showed that these nanoconjugates caused minimum damage to human keratinocyte cells (HaCaT cells) at 100 μg/mL, while also effectively reducing the cytopathogenicity of N. fowleri trophozoites to the HaCaT cells. The outcomes of our experiments have unveiled substantial potential for AND-AgNPs, BOR-AgNPs, and FOR-AgNPs in the realm of developing innovative alternative therapeutic agents to combat infections caused by N. fowleri. This study represents a significant step forward in the pursuit of advanced strategies for managing such amoebic infections, laying the foundation for the development of novel and more effective therapeutic modalities in the fight against free-living amoebae.