The determination of constitutive relation from the miniature tensile test is of high interest in multiple areas. Here, a convenient experimental method is proposed to determine the true stress–strain curve from the miniature tensile test. The instantaneous cross-sectional area is estimated by only one camera in aid of digital image correlation technique. This method was applied on commercial pure titanium and aluminum 6061 alloys, and the results indicate that the extracted true stress–strain curves are not scale-dependent. The derived mechanical properties from miniature specimens match well with the results of standard specimens. The correctness of the true stress–strain curve was evaluated by the finite element analysis method. The results suggest that the derived true stress–strain curve is capable to represent the constitutive behavior of the tested materials.