Miniature 3D Research Articles

Media Pembelajaran Miniatur Peta Kontur 3D bagi Siswa SMK Teknik Geospasial

The geospatial engineering skills program is a program that focuses on understanding surveying and mapping techniques. At SMK Negeri 1 Singosari in Cartography lessons for class X, learning uses conventional media and 2D printed maps, so efforts are needed to encourage active participation of students. This research aims to develop fun learning media for class X Geospatial Engineering students at SMK Negeri 1 Singosari. The research model applied in developing 3D contour map learning media is Research and Development. The research and development model is carried out using the ADDIE model. The resulting product is a miniature 3D contour map for class X cartography learning in Geospatial Engineering. The contour chosen in this media is the contour of the Mount Bromo area. This miniature 3D contour map media can be accessed using barcodes on Google Earth. The results of the assessment by material experts, media experts, small group and large group students showed a very good category. The learning media developed has advantages and disadvantages. The advantages of learning media are that it includes learning objectives, is equipped with access to the Google Earth application, and is equipped with guidelines on how to read 3D contour maps. Meanwhile, the weakness of this learning media is that it is large.

Discontinuous Atmospheric Pressure Interface in-Trap Single Photon Ionization Ion Trap Mass Spectrometry (DAPI in-Trap SPI ITMS)

A compact in-trap single photon ionization (SPI) system, termed discontinuous atmospheric pressure interface (DAPI) ion trap mass spectrometry (ITMS), for the rapid determination of trace volatile organic compounds (VOCs). DAPI enables the maintenance of high analyte pressure in the SPI source while preserving the vacuum. Utilizing vacuum ultraviolet (VUV) lamp as the ion source, we ionize the analyte within an enclosed miniature 3D ion trap for efficient analysis. Experimental findings demonstrate that the system achieves a mass resolution of less than 0.5 atomic mass units for full width at half maxima (FWHM), providing unit resolution. For the response for benzene, the system achieves almost instantaneous response, with rise and fall times of only 2 s, which is a significant advantage compared to the membrane inlet (MI) approach necessary for on-site detection. Additionally, tandem mass spectrometry (MS/MS) with unit m/z isolation window was achieved and resolved dimethyl disulfide and phenol with similar molecular weights. Our results demonstrate that the system provides excellent performance and we anticipate its application in rapidly determining VOCs.

Organoids as preclinical models of human disease: progress and applications.

In the field of biomedical research, organoids represent a remarkable advancement that has the potential to revolutionize our approach to studying human diseases even before clinical trials. Organoids are essentially miniature 3D models of specific organs or tissues, enabling scientists to investigate the causes of diseases, test new drugs, and explore personalized medicine within a controlled laboratory setting. Over the past decade, organoid technology has made substantial progress, allowing researchers to create highly detailed environments that closely mimic the human body. These organoids can be generated from various sources, including pluripotent stem cells, specialized tissue cells, andtumor tissue cells. This versatility enables scientists to replicate a wide range of diseases affecting different organ systems, effectively creating disease replicas in a laboratory dish. This exciting capability has provided us with unprecedented insights into the progression of diseases and how wecan develop improved treatments. In this paper, we willprovide an overview of the progress made in utilizing organoids as preclinical models, aiding our understanding and providing a more effective approach to addressing various human diseases.

Dual-plane miniature planar 3D ECT sensor based on 3 × 3 matrix electrode array

PurposeAs one of the tomographic imaging techniques, electrical capacitance tomography (ECT) is widely used in many industrial applications. While most ECT sensors have electrodes placed around a cylindrical chamber, the planar ECT sensor has been investigated for depth and defect detection. However, the planar ECT sensor has limited height and depth sensing capability due to its single-sided assessment with the use of only a single-plane design. The purpose of this paper is to investigate a dual-plane miniature planar 3D ECT sensor design using the 3 × 3 matrix electrode array.Design/methodology/approachThe sensitivity map of dual-plane miniature planar 3D ECT sensor was analysed using 3D visualisation, the singular value decomposition and the axial resolution analysis. Then, the sensor was fabricated for performance analysis based on 3D imaging experiments.FindingsThe sensitivity map analysis showed that the dual-plane miniature planar 3D ECT sensor has enhanced the height sensing capability, and it is less ill-posed in 3D image reconstruction. The dual-plane miniature planar 3D ECT sensor showed a 28% improvement in reconstructed 3D image quality as compared to the single-plane sensor set-up.Originality/valueThe 3 × 3 matrix electrode array has been proposed to use only the necessary electrode pair combinations for image reconstruction. Besides, the increase in number of electrodes from the dual-plane sensor setup improved the height reconstruction of the test sample.

Miniature planar 3D ECT sensor based on distributed electrode arrangement

Due to the low sensitivity in the middle of sensing chamber of planar peripheral electrode arrangement tomography system, a miniature planar electrical capacitance tomography (ECT) sensor with distributed electrode arrangement is proposed for imaging of 3D cultivated cells. In this work, the 3D imaging capability of the proposed sensor was compared to the peripheral electrode arrangement ECT sensor through numerical simulation of the sensitivity maps. The analysis of the sensitivity maps revealed that the sensitivity distribution of proposed sensor covered the entire surface of the sensing chamber which indicated improved sensitivity coverage in the sensing chamber. In addition, the axial resolution and the singular value decomposition (SVD) analysis of the sensitivity maps demonstrated that the proposed sensor has better 3D imaging capability. Then, the proposed sensor composed of nine electrodes, a thin PDMS layer and a glass coverslip was fabricated and investigated. The experimental results showed that the reconstructed 3D image by the proposed sensor was consistent at all the test positions. In contrast to the planar peripheral electrode arrangement ECT sensor that has an average correlation coefficient (CC) of 0.4571, the proposed sensor achieved a higher average CC of 0.5454, indicating better 3D imaging performance.

Gelatin-based cell culture device for construction and X-ray irradiation of a three-dimensional oral cancer model.

Bioassays using three-dimensional (3D) tissue models offer several advantages over 2D culture assays because they can reproduce the structure and function of native tissues. In this study, we used our newly designed gelatin device to generate a miniature 3D model of human oral squamous cell carcinoma with stroma and blood vessels. To enable air-liquid interface culture, we conceived a new device structure in which three wells were lined up and separated by a dividing thread; the wells could be connected by removing the dividing thread. Cells were seeded in the center well with the dividing thread to form a multilayer, followed by the supply of media from the side wells after thread removal. Human oral squamous cell carcinoma (HSC-4) cells, human umbilical vein endothelial cells (HUVECs), and normal human dermal fibroblasts (NHDFs) were successfully cocultured, resulting in structures that mimicked 3D-cancer tissues. This 3D-cancer model was subjected to an X-ray sensitivity assay, followed by the evaluation of DNA damage using confocal microscopy and section-scanning electron microscopy.

Magnetic Soft Millirobots 3D Printed by Circulating Vat Photopolymerization to Manipulate Droplets Containing Hazardous Agents for In Vitro Diagnostics.

3D printing via vat photopolymerization (VP) is a highly promising approach for fabricating magnetic soft millirobots (MSMRs) with accurate miniature 3D structures; however, magnetic filler materials added to resin either strongly interfere with the photon energy source or sediment too fast, resulting in the nonuniformity of the filler distribution or failed prints, which limits the application of VP. To this end, a circulating vat photopolymerization (CVP) platform that can print MSMRs with high uniformity, high particle loading, and strong magnetic response is presented. After extensive characterization of materials and 3D printed parts, it is found that SrFe12 O19 is an ideal magnetic filler for CVP and can be printed with 30% particle loading and high uniformity. By using CVP, various tethered and untethered MSMRs are 3D printed monolithically and demonstrate the capability of reversible 3D-to-3D transformation and liquid droplet manipulation in 3D, an important task for in vitro diagnostics that are not shown with conventional MSMRs. A fully automated liquid droplet handling platform that manipulates droplets with MSMR is presented for detecting carbapenem antibiotic resistance in hazardous biosamples as a proof of concept, and the results agree with the benchmark.

Ultra‐Compliant Indwelling Elastomer Balloons Improve Stability and Performance of Bioengineered Human Mini‐Hearts

Animal models used by the pharmaceutical industry to define drug safety and efficacy are expensive, resource‐intensive, subject to interspecies differences, and often fail to predict human responses. Therefore, engineered human tissue preparations are gaining importance as in vitro models to address translation from preclinical data to clinical outcome. Methods that improve fabrication consistency, usability, and physiological relevance of tissues are crucial. For cardiac applications, the goal is to form a miniature 3D chamber that mimics the physiology and biomechanical properties of the ventricle, allowing the measurement of clinically relevant endpoints, such as pressure–volume relationships, which cannot be obtained with simpler constructs. Despite advances in biofabrication, a perfusable mini‐ventricle with a competent endocardium remains an unmet challenge, resulting in thin‐walled organoids that are fluid permeable. A novel method is developed and validated for improving the stability and performance of the herein described human mini‐heart model by creating an ultra‐compliant elastomer balloon for hollow‐organ engineering applications. Balloon properties, tissue formation, and biological data are examined. Findings demonstrate a thin permanent lining, which is retained during testing and permits tissue contraction, functions to eliminate leakage, increase uniformity, and enable multiday longitudinal measurements. Biological data presented herein show reduced variability across measured cardiac parameters when compared to our previously published fabrication method.

The Effect of Family Caregiver Support used Three Dimension Magnetic Diabetic Food to Improve Diet Management

BACKGROUND: Diabetes mellitus (DM) Patient, especially the elderly in running a DM diet was often a major obstacle in the success of quality of life. Family caregiver support by using the miniature of three dimension (3D) magnetic diabetic food as a support for DM patient needed to help they DM diet. AIM: The purpose of this study was to analyze the effect of family caregiver support using miniature 3D magnetic diabetic food to was of dietary patterns of DM patients in elderly. METHODS: The study used was pre-experimental with a static-group comparison design. The research sample consisted of two groups, 36 DM patients in the treatment group and the control group, using a simple random sampling technique from the 90 elderly study populations. The variable was Family caregiver support using miniature magnetic 3D diabetic food and dietary patterns of DM patients in elderly. Data collect used 24-hour Food Recall Form questionnaire and Morisky Medication was Scale-8 Questionnaire . Data analysed using Wilcoxon Signed Rank Test and Man Whitney with alpha significance level <0.05. RESULTS: The results from the Wilcoxon Signed Rank Test statistic test showed a significant value of dietary patterns was showed p = 0.000. Different results between the control and treatment groups with the Whitney Man Test showed a value of p = 0.000. There was a difference in was to the diets of DM patients after family caregiver support for Media 3D Magnet. CONCLUSION: Family caregiver support using miniature 3D magnetic diabetic food can be used as an alternative to health education in improving patient was with DM diet management.

Recent advances in microarray 3D bioprinting for high-throughput spheroid and tissue culture and analysis.

Three-dimensional (3D) cell culture in vitro has proven to be more physiologically relevant than two-dimensional (2D) culture of cell monolayers, thus more predictive in assessing efficacy and toxicity of compounds. There have been several 3D cell culture techniques developed, which include spheroid and multicellular tissue cultures. Cell spheroids have been generated from single or multiple cell types cultured in ultralow attachment (ULA) well plates and hanging droplet plates. In general, cell spheroids are formed in a relatively short period of culture, in the absence of extracellular matrices (ECMs), via gravity-driven self-aggregation, thus having limited ability to self-organization in layered structure. On the other hand, multicellular tissue cultures including miniature tissues derived from pluripotent stem cells and adult stem cells (a.k.a. 'organoids') and 3D bioprinted tissue constructs require biomimetic hydrogels or ECMs and show highly ordered structure due to spontaneous self-organization of cells during differentiation and maturation processes. In this short review article, we summarize traditional methods of spheroid and multicellular tissue cultures as well as their technical challenges, and introduce how droplet-based, miniature 3D bioprinting ('microarray 3D bioprinting') can be used to improve assay throughput and reproducibility for high-throughput, predictive screening of compounds. Several platforms including a micropillar chip and a 384-pillar plate developed to facilitate miniature spheroid and tissue cultures via microarray 3D bioprinting are introduced. We excluded microphysiological systems (MPSs) in this article although they are important tissue models to simulate multiorgan interactions.

Cover Feature: Robotic Catalysis: A High‐Throughput Method for Miniature Screening of Mesoporous Metal Oxides (Chemistry ‐ Methods 4/2021)

The Cover Feature shows that a high-throughput method was developed for the screening of up to 96 mesoporous metal oxide catalysts. This system was created by employing robotics such as an acoustic liquid handler for the miniature catalyst formation and 3D printing. This proof of concept shows that mesoporous metal oxide could be miniaturised by the deposition of nanodroplet on a glass plate and screened for their activity in the oxidation of morin as a model reaction. More information can be found in the Full Paper Kariska Potgieter et al.

High-Throughput Screening of Compound Neurotoxicity Using 3D-Cultured Neural Stem Cells on a 384-Pillar Plate.

Assessing the neurotoxicity of test chemicals has typically been performed using two-dimensionally (2D)-cultured neuronal cell monolayers and animal models. The in vitro 2D cell models are simple and straightforward compared to animal models, which have the disadvantage of being relatively low throughput, expensive, and time consuming. Despite their extensive use in this area of neurotoxicology research, both models often do not accurately recapitulate human outcomes. To bridge this gap and attempt to better replicate what happens in vivo, three-dimensionally (3D) cultured neural stem cells (NSCs) encapsulated in hydrogels on a 384-pillar plate have been developed via miniature 3D bioprinting. This technology allows users to print NSCs on a pillar plate for rapid 3D cell culture as well as high-throughput compound screening. For this, the 384-pillar plate with bioprinted NSCs is sandwiched with a standard 384-well plate with growth medium for 3D culture, allowing researchers to expose the cells to test compounds and stain them with various fluorescent dyes for a suite of high-content imaging assays, including assays for DNA damage, mitochondrial impairment, cell membrane integrity, intracellular glutathione levels, and apoptosis. After acquiring cell images from an automated fluorescence microscope and extracting fluorescence intensities, researchers can obtain the IC50 value of each compound to evaluate critical parameters in neurotoxicity. Here, we provide a detailed description of protocols for cell printing on a 384-pillar plate, 3D NSC culture, compound testing, 3D cell staining, and image acquisition and analysis, which altogether will allow researchers to investigate mechanisms of compound neurotoxicity with 3D-cultured NSCs in a high-throughput manner. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Three-dimensional neural stem cell culture on a 384-pillar plate Basic Protocol 2: Compound treatment and cell staining Basic Protocol 3: Image acquisition, processing, and data analysis.

Analyzing the Performance of a Miniature 3D Wind Sensor for Mars.

This paper analyzes the behavior of a miniature 3D wind sensor designed for Mars atmosphere. The sensor is a spherical structure of 10 mm diameter divided in four sectors. By setting all the sectors to constant temperature, above that of the air, the 3D wind velocity vector can be measured. Two sets of experiments have been performed. First, an experimental campaign made under typical Mars conditions at the Aarhus Wind Tunnel Simulator is presented. The results demonstrate that both wind speed and angle can be efficiently measured, using a simple inverse algorithm. The effect of sudden wind changes is also analyzed and fast response times in the range of 0.7 s are obtained. The second set of experiments is focused on analyzing the performance of the sensor under extreme Martian wind conditions, reaching and going beyond the Dust Devil scale. To this purpose, both high-fidelity numerical simulations of fluid dynamics and heat transfer and experiments with the sensor have been performed. The results of the experiments, made for winds in the Reynolds number 1000–2000 range, which represent 65–130 m/s of wind speed under typical Mars conditions, further confirm the simulation predictions and show that it will be possible to successfully measure wind speed and direction even under these extreme regimes.

Motion-Sphere: Visual Representation of the Subtle Motion of Human Joints

Understanding and differentiating subtle human motion over time as sequential data is challenging. We propose Motion-sphere, which is a novel trajectory-based visualization technique, to represent human motion on a unit sphere. Motion-sphere adopts a two-fold approach for human motion visualization, namely a three-dimensional (3D) avatar to reconstruct the target motion and an interactive 3D unit sphere, that enables users to perceive subtle human motion as swing trajectories and color-coded miniature 3D models for twist. This also allows for the simultaneous visual comparison of two motions. Therefore, the technique is applicable in a wide range of applications, including rehabilitation, choreography, and physical fitness training. The current work validates the effectiveness of the proposed work with a user study in comparison with existing motion visualization methods. Our study’s findings show that Motion-sphere is informative in terms of quantifying the swing and twist movements. The Motion-sphere is validated in threefold ways: validation of motion reconstruction on the avatar, accuracy of swing, twist, and speed visualization, and the usability and learnability of the Motion-sphere. Multiple range of motions from an online open database are selectively chosen, such that all joint segments are covered. In all fronts, Motion-sphere fares well. Visualization on the 3D unit sphere and the reconstructed 3D avatar make it intuitive to understand the nature of human motion.

A Miniature 3D Printed On-Off Linear Pneumatic Actuator and Its Demonstration into a Cartoon Character of a Hopping Lamp

Although 3D printing has been extensively used to create passive machines and mechanisms, 3D printing of actuators is a relatively new concept. 3D printing of actuators allows greater customization, accelerates the design and development, and consequently saves time and money. We present the design and fabrication of a 3D printed, miniature size, double-acting, On-Off type, linear pneumatic actuator. The actuator has an overall length of 8 cm, a bore size of 1.5 cm, and a stroke length of 2.0 cm. The overall weight is 12 gm and it generates a peak output power of 2 W when operating at an input air pressure of 40 psi ( 275 . 79 kPa). This paper demonstrates novel methods to solve the challenges that arise during fabrication that include: (1) chemical post-processing to achieve airtight sealing and a smooth surface finish, (2) strategic placement of a metallic part within 3D printed plastic for higher strength, (3) design of an airtight seal between the cylinder and piston head, (4) chemical bonding of printed parts using adhesive, and (5) use of a lubricant to reduce friction and improve force generation. The power-to-weight ratio of our actuator is comparable to that of high-end commercial actuators of similar size. The utility of the actuator is demonstrated in a series of jumping experiments with the actuator and by incorporating the actuator into a hopping robot inspired by Disney/Pixar Luxo lamp. We conclude that 3D printed pneumatic actuators combine the high power of pneumatics with the low weight of plastics, and structural strength through the selective placement of metal parts, thus offering a promising actuator for robotic applications.

Effects of the Size of Mixed-Reality Person Representations on Stress and Presence in Telecommunication

We study how mixed reality (MR) telepresence can enhance long-distance human interaction and how altering 3D representations of a remote person can be used to modulate stress and anxiety during social interactions. To do so, we developed an MR telepresence system employing commodity depth sensors and Microsoft’s Hololens. A textured, polygonal 3D model of a person was reconstructed in real time and transmitted over network for rendering in remote location using HoloLens. In this study, we used mock job interview paradigm to induce stress in human–subjects interacting with an interviewer presented as an MR hologram. Participants were exposed to three different types of real-time reconstructed virtual holograms of the interviewer, a natural-sized 3D reconstruction (NR), a miniature 3D reconstruction (SR) and a 2D-display representation (LCD). Participants reported their subjective experience through questionnaires, while their biophysical responses were recorded. We found that the size of 3D representation of a remote interviewer had a significant effect on participants’ stress levels and their sense of presence. The questionnaire data showed that NR condition induced more stress and presence than SR condition and was significantly different from LCD condition. We also found consistent patterns in the biophysical data.

Controllable Wrinkling Patterns on Chitosan Microspheres Generated from Self-Assembling Metal Nanoparticles.

Materials with surface wrinkles at a micro/nanoscale possess extraordinary fascinating properties, and various techniques have been employed to create controllable wrinkles. Herein, natural polysaccharide was used to construct the surface wrinkled microsphere with controllable wrinkling patterns. A robust microsphere with an average size of about 55 μm fabricated from chitosan in alkali/urea aqueous solution was swelled and then coated orderly by introducing rigid silver nanoparticles (Ag NPs) with an average size of about 5 nm as the shell onto the surface through electrostatic layer-by-layer (LBL) self-assembly followed by deswelling, resulting in a surface wrinkled microsphere. The significant difference in the swelling behaviors between the stiff Ag shell and swelled chitosan microsphere could generate enough driving forces to form 3D micro- and nanoscale wrinkling surface topography. The surface wrinkled microspheres exhibited the hierarchically porous structure and hydrophobicity, and the topographical patterns could be adjusted by controlling the thickness of the Ag NP layer to achieve the sizes of wrinkling ranging from 60 to 300 nm. It was demonstrated that the wrinkled microspheres were superior as 3D surface-enhanced Raman spectroscopy (SERS) substrates, in which the wrinkled structure with spatial periodicity was proved to be effective for enhancing the SERS signal. The microsphere with controllable wrinkled surface topography could be applied to be a miniature 3D device, which promises potential technological applications in various areas.

Acoustic characterization of a miniature matrix transducer for pediatric 3D transesophageal echocardiography

This paper presents the design, fabrication and characterization of a miniature PZT-on-CMOS matrix transducer for real-time pediatric 3-dimensional (3D) transesophageal echocardiography (TEE). This 3D TEE probe consists of a 32 × 32 array of PZT elements integrated on top of an Application Specific Integrated Circuit (ASIC). We propose a partitioned transmit/receive array architecture wherein the 8 × 8 transmitter elements, located at the centre of the array, are directly wired out and the remaining receive elements are grouped into 96 sub-arrays of 3 × 3 elements. The echoes received by these sub-groups are locally processed by micro-beamformer circuits in the ASIC that allow pre-steering up to ±37°. The PZT-on-CMOS matrix transducer has been characterized acoustically and has a centre frequency of 5.8 MHz, -6 dB bandwidth of 67%, a transmit efficiency of 6 kPa/V at 30 mm, and a receive dynamic range of 85 dB with minimum and maximum detectable pressures of 5 Pa and 84 kPa respectively. The properties are very suitable for a miniature pediatric real-time 3D TEE probe.

A 3D Printed Membrane-Based Gas Microflow Regulator for On-Chip Cell Culture

A miniature 3D printed membrane-based gas microflow regulator which delivers gaseous media to on-chip cell cultures is presented in this paper. The device uses a polydimethylosiloxane (PDMS) membrane to act as a diffusion barrier and maintain gas flow at the desired rate. The regulator was characterized, and repeatable flow values for different membrane thicknesses and gas types in the function of pressure were obtained. As a result, a long-term on-chip culture of Euglena gracilis was achieved, this was due to constant and stable carbon dioxide release from the regulator (flow rate: 0.3 μL/min).

Liquid Viscosity Measurement Using a Vibrating Flexure Hinged Structure and a Fiber-Optic Sensor

A novel viscosity measurement system based on a miniature 3D printed parallelogram flexure hinge structure and a fiber-optic sensor is developed. Lorentz forces are applied to excite the structure vibration at various frequencies, and the fiber-optic sensor is used to measure the amplitude and phase responses without the electromagnetic interference. The system is modeled as a single oscillator by taking into account the liquid-induced mass and damping as well as the additive stiffness due to the liquid viscoelasticity. By curve fitting of the experimentally obtained amplitude and phase responses based on the analytical model, the viscosity and the additive stiffness can be obtained. This method can help differentiate the effect of the viscosity and elasticity on the response of the sensing structure. By implementing this method, accurate viscosity measurements over a large range of 1–1045 $\text {mPa} \cdot \text {s}$ are demonstrated with the errors of <3.7% for all the test samples. It is also confirmed in the experiment that the liquid-induced additive stiffness is strongly related to the liquid storage modulus.