Impacts of four amino acids, viz. lauroyl glutamine, lauroyl glycine, lauroyl alanine, and oleoyl glycine, on biodegradability, oxidation stability, and corrosiveness of HVI 350 mineral lubricating oil were studied. The biodegradabilities of neat lubricating oil and its formulations with the amino acids were evaluated on a biodegradation tester and the microbial characters in the biodegradation sewage observed through a microscope. Furthermore, the oxidation stability and corrosiveness of neat oil and the formulated oils were determined on an oxidation tester and a copper strip corrosion tester, respectively. The results indicated that the amino acids markedly enhanced biodegradability of unreadily biodegradable mineral lubricating oil by promoting microbe production in the biodegradation processes, thus accelerating biodegradation of mineral lubricating oil. On the other hand, the amino acids to some extent retarded oxidation of mineral lubricating oil by inhibiting increases of acidity, viscosity and insolubles during oxidation. Otherwise, excellent color ratings of copper strips, namely, 1b for neat oil and 1a for all the formulated oils, were obtained in the corrosion tests, demonstrating that the corrosiveness of both neat oil and the amino acid-doped oils was negligible, although the formulated oils seemed to provide slightly better anticorrosion ability.