Source Of Milk Research Articles

Environmental and behavioural exposure pathways associated with diarrhoea and enteric pathogen detection in 5-month-old, periurban Kenyan infants: a cross-sectional study

ObjectivesThe aim of this study was to test whether household environmental hygiene and behavioural conditions moderated associations between diarrhoea and enteric pathogen detection in infants 5 months of age in...

53 The Role of Veterinary Services on Nutrition Schemes, Management, and Health Practices for Sheep and Goats in Rural Ngaka Modiri Molema District Municipality, Northwest Province of South Africa

Abstract Poverty is a feature of rural households, and as a result, rural folks usually adopt low-input agriculture to alleviate poverty. Many households in Ngaka Modiri Molema District keep animals, particularly sheep and goats, for a variety of reasons, including as source of income, milk, and meat. However, they lack the management skills that are necessary for their livestock to increase their productivity. Inadequate animal management, including poor feeding and housing standards and poor application of proper animal health procedures are compounded by a lack of agricultural experience and low-input farming methods. In addition, poor animal nutrition, sickness and a high prevalence of parasites, and low animal production output further contribute to low overall productivity. Therefore, support offered by the veterinary services, and the impact these interventions have on sheep and goat farming among rural farmers need to be investigated. The present study will seek to describe the feeding schemes and management practices adopted by sheep and goat owners, as well as the role played by veterinary and extension services in the development of such practices. Health care provided to sheep goat farmers by the veterinary services will also be determined. A survey will be conducted in Ngaka Modiri Molema District Municipality, a rural area in North West Province of South Africa. The study population will consist of all farmers rearing sheep and goats, both female and male. Animal and farm information level data and general farm management practices will be collected via a questionnaire survey. Using the Statistical Kit for STATA (version 16), data will be examined by computing frequency, percentages, and proportions based on the characteristics of the sample population. This survey will help to provide opportunities for resource-poor farmers in rural areas to develop their flocks.

Bio-inspired one-dimensional based textile fabric coating for integrating high flame retardancy, antibacterial, toxic gases suppression, antiviral and reinforcement properties

Herein, a green synthesis approach has been used for development of innovative, renewable, efficient and cost-effective flame retardant, antivirus, reinforced and antibacterial coatings for textile fabrics. Hence, rennet casein as biopolymer was facilely prepared from renewable caw milk source and its solution was prepared and then, abundant halloysite nanotubes (HNTs) were uniformly dispersed with aid of ultrasonication producing new one-dimensional nanocomposite. Afterwards, the developed coatings were coated on different textile fabrics. The mass loadings of HNTs dispersed in casein solution were varied. The flammability, toxic gases suppression, reinforcement, antibacterial, and antiviral properties of the coated textile fabrics were significantly enhanced. The flame retardancy of treated textile fabrics achieved zero rate of burning (horizontal test) compared to 119 mm/min for blank cotton textile recording high-class flame-retardant textile. Additionally, the flame retardancy was corroborated from high LOI value of 35% compared to 18.5% for blank sample. Interestingly, the coated textile fabrics recorded significant inhibition of bacterial growth recording 17.6 mm of clear inhibition zone compared to zero for virgin and casein coated samples. Interestingly, the as developed coating affords more than 90% antiviral inhibition for adenovirus and herpesvirus. This is in addition to significant reinforcement of more than two-fold increase in tensile strength achieving excellent reinforcement. Interestingly, the developed coated textile fabrics achieved outstanding toxic gases suppression after combustion recording reduction in emission in CO, CO2, SO2, NOx and NO, by 97,70,85,75,75% respectively. Flame retardancy and toxic gases suppression mechanism was discussed and elucidated.

Combined Effect of Milk Source and Acidification Method of Cheese Milk on Properties of Mozzarella Cheese

A 3×3 factorial arrangement of treatments in a completely randomized design was used to find out the combined effect of milk source {cow (CM), buffalo (BM) and mixed (CM:BM 1:1 ratio)} and the method of acidification {starter culture (SC): Streptococcus thermophilus, acetic acid (AA) and citric acid (CA)} of cheese milk on properties of Mozzarella cheese. Cheese made using BM and acidified with SC served as the control. Main effects of milk source and method of acidification of cheese milk showed a significant effect on yield of cheese whereas interaction effect was not observed. A significant interaction effect between milk source and method of acidification of cheese milk was observed for fat and protein percentages, meltability, b* value and sensory properties of resultant cheese. Fat and protein content was significantly higher in cheese made from BM acidified using AA and in control, respectively. Meltability was superior in Mozzarella cheese manufactured from CM acidified using CA compared to the control. Sensorily, Mozzarella cheese manufactured from mixed milk (CM: BM 1:1 ratio) acidified using CA obtained the highest mean score for all the sensory attributes and was significantly superior compared to the control. Therefore, the milk source and the method of acidification of cheese milk are closely linked to the yield and the quality characteristics of Mozzarella cheese and hence, careful selection of raw materials and manipulation of processing conditions are required to get an optimum quality end product.

Source of human milk (mother or donor) is more important than fortifier type (human or bovine) in shaping the preterm infant microbiome

SummaryMilk fortifiers help meet the nutritional needs of preterm infants receiving their mother’s own milk (MOM) or donor human milk. We conducted a randomized clinical trial (NCT03214822) in 30 very low birth weight premature neonates comparing bovine-derived human milk fortifier (BHMF) versus human-derived fortifier (H2MF). We found that fortifier type does not affect the overall microbiome, although H2MF infants were less often colonized by an unclassified member of Clostridiales Family XI. Secondary analyses show that MOM intake is strongly associated with weight gain and microbiota composition, including Bifidobacterium, Veillonella, and Propionibacterium enrichment. Finally, we show that while oxidative stress (urinary F2-isoprostanes) is not affected by fortifier type or MOM intake, fecal calprotectin is higher in H2MF infants and lower in those consuming more MOM. Overall, the source of human milk (mother versus donor) appears more important than the type of milk fortifier (human versus bovine) in shaping preterm infant gut microbiota.

Choice Experiment Assessment of Consumer Preferences for Yogurt Products Attributes: Evidence from Taiwan.

Previous studies on consumer yogurt preferences have mainly focused on added sugar, nutrient content, and health claims, leaving several knowledge gaps that should be filled through in-depth research. In this study, a more complete multi-attribute preference model was developed using the number of probiotic types, type of milk source, presence of edible gels (GEL), and usage of health food labels as the main yogurt attributes. A choice experiment (CE) was then conducted to investigate the relationship between multiple attribute preferences and willingness-to-pay (WTP). A total of 435 valid questionnaires were collected by the convenience sampling method. The results show that (1) respondents highly value the health food label (HEA), followed by the number of probiotic types (PRO); (2) the highest WTP in the conditional logit (CL) model was New Taiwan Dollar (NTD) (USD 10.5 for HEA, and the lowest was NTD 1.0 for 100% milk powder (MLK2); (3) in the random-parameter logit (RPL) model, the highest WTP was NTD 14.6 for HEA, and the lowest was NTD 2.8 for GEL; (4) the most preferred attribute combination of yogurt was “8 or more probiotic types”, “a blend of raw milk and milk powder”, “the absence of edible gels”, “the presence of a health food label”, and “a price premium of NTD 6–10”; (5) married respondents with children were more willing to pay extra for yogurt products with a higher number of probiotic types and a health food label. The results may help the food industry understand and pay attention to consumer needs, which will, in turn, provide a reference for future product development and marketing strategies.

Development of Functional Fermented Dairy Products Containing Taiwan Djulis (Chenopodium formosanum Koidz.) in Regulating Glucose Utilization

Taiwan djulis (Chenopodium formosanum Koidz.) is a plant native to Taiwan and is a grain rich in nutrients, vitamins, and minerals with antioxidant properties. This paper aimed to use appropriate processing technology and incorporate probiotics, thus combining Taiwan’s high-quality milk sources to develop Taiwan djulis fermented dairy products. Later, FL83B cells have used to evaluate the glucose utilization ability after the administration of djulis. We first screened Lactiplantibacillus plantarum and combined it with the traditional yogurt strains Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus for cultivation. Further, the fermentation process was optimized where 7.5% djulis and an inoculum of 107 colony forming unit/mL were fermented at 40 °C for 18 h. Compared to fermented milk without djulis, the analysis of various nutrients and active ingredients showed that free radical scavenging abilities of DPPH and ABTS reached 2.3 and 2.0 times (752.35 ± 29.29 µg and 771.52 ± 3.79 µg TE/g, respectively). The free phenol content increased 2.5 times (169.90 ± 14.59 mg gallic acid/g); the total flavonoid content enhanced 4.8 times (3.05 ± 0.03 mg quercetin/g), and the gamma-aminobutyric acid content was 3.07 ± 0.94 mg/g. In a co-culture of mouse liver cells with fermented products, 100 ppm ethanol extract of fermented products effectively improved glucose utilization with increased glucose transporter expression. This functional fermented dairy product can be developed into the high value added local agricultural products and enhance multiple applications including medical and therapeutic fields.

Blood Trace Element Status in Camels: A Review.

Simple SummaryTrace elements are required in small quantities for all species, and their role in many biological functions is essential. Additionally, their deficiency or excess can have important consequences for metabolism and health. Although camels live in ecosystems dominated by minerals, they are also subject to such imbalances. To investigate the trace element status of camels, as for other animals, blood sampling is preferred. The present paper gives a large overview of the values observed in camel blood samples under different physiological and health conditions, as well as some data regarding the symptoms in deficiency or excess situations. The main trace elements involved are copper, zinc, iron, selenium, manganese, cobalt, iodine, fluorine and molybdenum. In addition, some data regarding bromide and nickel, due to their specific mention in the literature, are included. Although the status of sulfur is regarded as intermediary between the main and trace elements, it is added due to its role in polluted areas. Finally, some specificities of camel trace elements are discussed.Trace minerals play an important role in animal health and productivity. They are involved also in many physiological activities, and their deficiency causes a variety of pathological problems and metabolic defects, reducing consequently the animal productivity. The demand for animal products in semi-arid areas is rapidly increasing, and the supply is still below the required level, partially due to low animal productivity. Camels (Camelus dromedarius and Camelus bactrianus) are considered one of the main sources of healthy, high-quality meat and milk for human consumption within most of the countries in the semi-arid regions. Despite their efficient adaptation to their environment, camels can suffer from the growth retardation of newborns, low feed efficiency, anemia, poor fertility, poor reproduction and many other metabolic disorders. It is well known that trace mineral deficiencies and trace mineral toxicities can influence camels’ production and reproductive efficiency, as well as many aspects of their growth and metabolism. Evaluating the trace minerals status of camels and their variability is an obvious step toward improving camels’ productivity and health. Thus, the present article reviews the data regarding the status of trace minerals (copper, zinc, iron, selenium, manganese, cobalt, iodine, fluorine, molybdenum, sulfur, bromide and nickel) in camel blood and their physiological variability, with a focus on their deficiency and toxicity effects.

Human Milk Sharing in the United States: A Scoping Review.

Background: Human milk is the optimal form of infant nutrition. If mother's own milk is unavailable, families may seek alternative sources of human milk through milk sharing, despite potential health and safety risks with this practice. Objective: The purpose of this scoping review was to synthesize the current literature on human milk sharing in the United States to help health care professionals better understand how families may use this practice for infant nutrition. Methods: A systematic search was conducted in June 2021 using Ovid MEDLINE, Embase, CINAHL, ProQuest Central, Web of Science, and Google Scholar. Articles were included if the primary outcome was milk sharing, excluding milk banks and preterm hospitalized infants. Results were limited to studies conducted in the United States and published in English after January 1, 2000. Results: From 2,124 articles, 34 met inclusion criteria and were reviewed. Study designs were largely observational (30/34), and participants were predominantly white, married, and middle-income women experiencing lactation problems or in possession of excess breast milk. Milk sharing, not for profit, was often facilitated through the internet but exchanged in person. Conversely, for-profit milk sharing often involved shipping and studies found evidence of milk contamination. No studies documented infant harm. Conclusions: There is limited research on the milk quality and relative risks of milk sharing, and no research on clinical outcomes in infants fed shared milk. Clinicians have opportunities to engage in open conversations about this practice to guide risk mitigation, however, research on recipient infant outcomes are urgently needed to inform best practices.

Zoonotic diseases risk perceptions and protective behaviors of consumers associated with consumption of meat and milk in and around Bishoftu, Ethiopia

Consumption of raw or undercooked milk and meat is a major source of zoonoses. Information on the public risk perceptions and protective behaviors are essential for prevention and control of these diseases. However, such information is limited in Ethiopia. We assessed the risk perceptions and protective behaviors of the community toward zoonoses associated with consumption of raw meat and milk by employing the basic constructs of food safety health belief model. We collected data from 210 respondents (105 from each urban and peri-urban) using a face-to-face questionnaire interview. Majority of the respondents (96.2%) had knowledge about zoonoses. Despite their knowledge, 91% and 94.3% had raw meat and raw milk consumption habits, respectively. The odds of raw meat consumption was significantly higher in males (AOR = 3.90; CI = 1.28–11.86) and raw milk consumption was higher in females (AOR = 3.82; CI = 0.78–18.65). About 65% of respondents, obtained meat from backyard slaughtering. Self-owned and smallholder dairy farms are the major sources of milk for peri-urban and urban dwellers, respectively. While 46.7% of the respondents reported that community members are the primary sources of information, only 2.4% reported animal health professionals as their primary source of information. More than half of the respondents either moderately or strongly agreed that consumption of raw meat and raw milk can expose them to zoonoses. Urban dwellers had perceived more seriousness of consuming raw animal products. Peri-urban residents had significantly lower intention than urban ones to implement protective behaviors such as stopping consuming raw meat (P = 0.017) and milk (P = 0.043). We noted that lack of access to refrigerator and pasteurized milk were the perceived barriers for protection against zoonoses among the peri-urban dwellers. There was significant difference in perceived benefits of avoiding consumption of raw meat (P = 0.005) and milk (P = 0.001) between urban and peri-urban residents. Our study showed that irrespective of knowledge about zoonoses, consumptions of raw meat and raw milk remained common practices among the respondents. Public health education on the risk of consumption of raw milk and meat and the significance of protective behaviors using a one-health approach is critically needed to ensure meat and milk safety.

More evidence: Mothers’ own milk is personalized medicine for very low birthweight infants

“Human milk sources and fortification: Impact on fecal microbiota and calprotectin in premature infants” highlights improved outcomes in predominantly mothers’ own milk-fed versus predominantly pasteurized donor human milk-fed VLBW infants, regardless of fortifier type. Research and practice implications are reviewed.

On-the-Pack Voluntary Well-Being Messaging for Milks Targeting Chinese Older Adults: A Content Analysis.

China is experiencing severe population aging. Given that milks targeting older adults are one of the most popular foods designed for Chinese older adults (COA), this study investigated on-the-pack (OTP) voluntary well-being messaging (VWM, ways of communicating a product’s broad well-being benefits through information on food content or statements linked to favourable components, functions, or well-being outcomes) for milk targeting COA. Over 200 products identified from two sources (JD.com and Mintel’s global new products database), were analysed for type, content, and VWM frequency for different brand origins and milk sources of various animal species, nutrition claim regulation compliance and alignment with nutrition facts. The results suggested: (1) different brand origins (domestic vs. international) and milk source (cow vs. goat) highlighted different well-being aspects of products, (2) three products failed to comply with government regulations made for nutrition labelling of pre-packed foods (GB 28050-2011), but (3) excepting fat, all ‘contains’ claims and most ‘high’ claims did not reflect significantly greater levels of nutrients, compared to products with no claims. The findings create a comprehensive picture of OTP VWM for milks targeting older adults in China, providing useful information for consumer, domestic, and international dairy industries, and policymakers.

Cynara cardunculus as a potential source of milk coagulating protease: Effects on physical properties of cow's milk.

In the present research study, Cynara cardunculus (wild cardoon) flowers were blended and extracted using different types of buffers (phosphate buffer; citrate buffer and distilled water) for different maceration times. The most reliable, quick and efficient buffer was found to be phosphate (pH=6.5) with a 6-h maceration time, which was used throughout this study. C. cardunculus extract (CE) was found to have high clotting and proteolytic activities. The extracted enzyme was found to be very stable against a wide range of pH values as well as of temperature. The formation of milk gels prepared in the presence of CE with different types of milk was evaluated using dynamic rheology and Turbiscan. The evolution of both elastic (G') and viscous (G″) moduli was monitored with time. The values of the whole milk enriched with milk powder gels were higher. Coagulum stability was evaluated using Turbiscan. The textural properties and the curd-firming rate of coagulum were also determined. In conclusion, the prepared CE could be an efficient milk-clotting agent in the production of dairy products.

Does Food Intake of Australian Toddlers 12-24 Months Align with Recommendations: Findings from the Australian Feeding Infants and Toddlers Study (OzFITS) 2021.

(1) Background: Food-based dietary guidelines promote population health and well-being through dietary patterns that reduce chronic disease risk while providing adequate energy and nutrients. In Australia, recommended dietary patterns based on servings per day from the five food groups—fruits, vegetables, cereals and grains, meats and alternatives, and dairy—have been developed for toddlers 1–2 years of age. However, no study has assessed the intake of the five food groups in this age group nationally. (2) Aim: To compare daily servings and the percentage of energy from the five food groups and discretionary foods in toddlers 1–2 years old to the Australian Dietary Guidelines. (3) Methods: Dietary intake was assessed using a one-day food record for 475 toddlers. (4) Results: Apart from fruit and dairy, servings of the five food groups were below the recommendations. Two-thirds of toddlers did not consume enough vegetables, and only 10% consumed the recommended number of servings for cereals and grains. On average, toddlers consumed only half the recommended servings of meat and alternatives. Nearly all toddlers (89%) consumed discretionary foods, which accounted for ~12% of total energy. Forty-five percent of toddlers received breastmilk. On average, breastfed toddlers consumed fewer servings from the five food groups than non-breastfed toddlers. Dairy contributed 20% of daily energy in all toddlers; however, this food group accounted for 13% in breastfed and 32% in non-breastfed toddlers on the day of the food record. (4) Conclusions: Compared to the recommendations, alignment with the servings of the five food group foods was not achieved by most toddlers, except for fruit and dairy. Discretionary foods may have displaced nutritious family foods. Consistent with Australian Infant Feeding Guidelines, many toddlers in this study continued to receive breastmilk but the recommended dietary patterns do not include breastmilk. Dietary modeling, including breastmilk as the primary milk source, is urgently needed, along with practical advice on incorporating breastmilk in a toddler’s diet while optimizing food consumption.

Microbiological contamination, antimicrobial residues, and antimicrobial resistance in raw bovine milk in Lebanon

A total of 195 raw milk samples were collected over two consecutive years from 48 milk sources in Lebanon. Collected samples were examined for microbiological quality and antibiotic susceptibility. Results revealed non-conformity with local and international standards, in 96% of samples for total aerobic mesophilic bacteria (TAMB), 81% for total coliform (TC), 17% for Staphylococcus aureus (S. aureus), 35% for Salmonella spp., and 65% for Escherichia coli (E. coli). Antimicrobial residues of oxytetracycline, tetracycline, ciprofloxacin, sulfamethazine, and doxycycline were below the maximum residue limits (MRL). On the other hand, E. coli isolates showed resistance to tetracycline (41%), doxycycline (39%), nalidixic acid (20%), and ciprofloxacin (3%), while S. aureus isolates were found resistant to doxycycline (63%), and tetracycline (51%). For Salmonella spp., isolates were found to be resistant to doxycycline (12%), tetracycline (6%), and nalidixic acid (4%). In conclusion, sampled milk shows high levels of pathogenic bacteria and antimicrobial resistance.

Comparative Mass Spectrometry Analysis and Immunomodulatory Effects of Casein Glycomacropeptide O-Glycans in Bovine and Caprine Whey Powder.

Casein glycomacropeptide carries various O-glycan modifications, which, together with variations in the amino acid composition of the glycopeptide, may result in different biological activities. In this study, O-glycans of casein glycomacropeptide from bovine and caprine whey powder were qualitatively and quantitatively analyzed by LC-UV-ESI-MS/MS, and their immune activities and regulatory mechanisms were compared. O-Glycans' total content was 1.54 times higher in bovine than in caprine glycomacropeptide. The glycoform H1N1S2 (H: hexose; N: N-acetylgalactosamine; and S: N-acetylneuraminic acid) accounted for nearly 50% of total glycomacropeptide O-glycans in bovine milk but less than 20% in caprine milk. Bovine glycomacropeptide glycosylation promoted the immune activity of RAW264.7 cells, which may be linked to a higher content of disialylated O-glycans. Glycomacropeptide from both milk sources significantly upregulated the mRNA expression of IL-1α, TNF-α, and IL-10 in RAW264.7 cells and activated the MAPK immunomodulatory signaling pathway. This study demonstrates the possible use of casein glycomacropeptide as an immunomodulatory agent.

Development of a control strategy towards elimination of Trypanosoma evansi infection (surra) in camels in Africa

With an increasing worldwide population that presently exceeds 38 million, camels are important source of meat, milk, and transportation of goods, in many regions of the world. Camels are particularly critical in the northern parts of Africa, above the tsetse belt. However, camel breeding areas are expanding into southern areas, under the pressures of global warming, leading to increasing risk of acquiring parasitic infections in these non-traditional ecotypes. Common biting flies (tabanids, stomoxyine flies, and Hippobosca camelina) act as mechanical vectors, resulting in exposure to trypanosomosis (Trypanosoma evansi; Surra) and high camel morbidity and mortality. In these regions, complicating infections with other Trypanosoma may also occur, particularly Trypanosoma vivax. In many modern camel-breeding areas, human populations are living under political upheaval (terrorism, riots), poverty, and precarity (drought, climate modification). Hence, control and/or elimination of Surra in camels would be beneficial to the economies of these populations. Due to the relatively straightforward epidemiology (single parasite with seasonal transmission in a single host species), control of Surra in Africa is affordable and should be based on implementing: (1) national veterinary services capabilities; (2) efficient diagnosis and control methods; (3) joint integrated control of Surra, gastrointestinal helminthoses (mainly haemonchosis), and sarcoptic mange. We propose that methods to control two economically-critical disease problems, gastrointestinal parasitosis and sarcoptic mange, will support improved Surra control in camels. Aided by decision-makers and donors, elimination of Surra could improve camel health and productivity, and stabilize camel-rearing in regions of the world that suffer from political instability and global warming pressures.

Genome-wide identification of long noncoding RNA genes and their potential association with mammary gland development in water buffalo.

Water buffalo, an important domestic animal in tropical and subtropical regions, play an important role in agricultural economy. It is an important source for milk, meat, horns, skin, and draft power, especially its rich milk that is the great source of cream, butter, yogurt, and many cheeses. In recent years, long noncoding RNAs (lncRNAs) have been reported to play pivotal roles in many biological processes. Previous studies for the mammary gland development of water buffalo mainly focus on protein coding genes. However, lncRNAs of water buffalo remain poorly understood, and the regulation relationship between mammary gland development/milk production traits and lncRNA expression is also unclear. Here, we sequenced 22 samples of the milk somatic cells from three lactation stages and integrated the current annotation and identified 7,962 lncRNA genes. By comparing the lncRNA genes of the water buffalo in the early, peak, and late different lactation stages, we found that lncRNA gene lnc-bbug14207 displayed significantly different expression between early and late lactation stages. And lnc-bbug14207 may regulate neighboring milk fat globule-EGF factor 8 (MFG-E8) and hyaluronan and proteoglycan link protein 3 (HAPLN3) protein coding genes, which are vital for mammary gland development. This study provides the first genome-wide identification of water buffalo lncRNAs and unveils the potential lncRNAs that impact mammary gland development.

Harnessing Food Product Reviews for Personalizing Sweetness Levels.

Sweet taste is innately appealing, ensuring that mammals are attracted to the sweetness of mother’s milk and other sources of carbohydrates and calories. In the modern world, the availability of sugars and sweeteners and the eagerness of the food industry to maximize palatability, result in an abundance of sweet food products, which poses a major health challenge. The aim of the current study is to analyze sweetness levels, liking, and ingredients of online reviews of food products, in order to obtain insights into sensory nutrition and to identify new opportunities for reconciling the palatability–healthiness tension. We collected over 200,000 reviews of ~30,000 products on Amazon dated from 2002 to 2012 and ~350,000 reviews of ~2400 products on iHerb from 2006 to 2021. The reviews were classified and analyzed using manual curation, natural language processing, and machine learning. In total, ~32,000 (Amazon) and ~29,000 (iHerb) of these reviews mention sweetness, with 2200 and 4600 reviews referring to the purchased products as oversweet. Oversweet reviews were dispersed among consumers. Products that included sucralose had more oversweet reviews than average. 26 products had at least 50 reviews for which at least 10% were oversweet. For these products, the average liking by consumers reporting oversweetness was significantly lower (by 0.9 stars on average on a 1 to 5 stars scale) than by the rest of the consumers. In summary, oversweetness appears in 7–16% of the sweetness-related reviews and is less liked, which suggests an opportunity for customized products with reduced sweetness. These products will be simultaneously healthier and tastier for a substantial subgroup of customers and will benefit the manufacturer by expanding the products’ target audience. Analysis of consumers’ reviews of marketed food products offers new ways to obtain informative sensory data.

Immunological and Hemato-biochemical alterations in diarrhoeic buffaloes screened for Mycobacterium avium subspecies paratuberculosis infection using ‘indigenous ELISA kit’

Paratuberculosis (ParaTB) in ruminants, commonly known as Johne’s disease (JD)is caused by Mycobacterium avium subspecies paratuberculosis (MAP). Characteristic hallmark of JD is chronic inflammation of intestines. Similar infection of MAP in human population is called Crohn's disease (CD). In sub-tropical countries like India, domestic buffaloes are important source of milk and meat and vital for thenutritional security of human beings. Diarrhoea leading to weight loss and weakness invariably leads to reduced productivity, therefore huge financial losses to the livestock farmers. Evaluation of immuno-biochemical alterations and their management through appropriate therapeutic interventions would aid in preventing production losses caused by diarrhoea following MAP infection.Most of the studies were performed to understand immune-biochemical alterations in serum samples of cattle following experimental infections. But studies are limited in clinical cases of buffaloes suffering from diarrhoea and were suspected for MAP infection.We categorized diarrhoeic buffaloes brought at Veterinary Clinical Complex of Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, for therapeutic intervention; As MAP negatives / suspected, low positive, positive and strong positive based on indigenous ELISA kit results. We further compared immuno hemato-biochemical alterations in MAP negative/suspected low positive, positive and strong positive animals.Our major findings were; significant differences observed in creatinine, globulin, total leucocytic, neutrophils and in lymphocytes numbers. Decreasing trend in leucocytes was seen from suspected to strong positive animals, which indicated the progression of disease towards chronic paratuberculosis. However, differences were not observed in the Th1 cytokine levels.