Milk Of Other Species Research Articles

Whey proteins in donkey milk

In recent years, by-products obtained from farm animals are of increasing interest to farmers and once again to the population, which have properties that are not to be neglected by consumers. Milk is one of the main sources of essential compounds, and the by-products obtained from its processing are important elements in human nutrition, bringing an important contribution of elements to the body. Whey is a complex by-product with a high protein content, and in the case of donkey milk, important benefits have been highlighted, such as antimicrobial, antiviral and anti-allergenic properties. The cost of whey from donkey milk is currently low, which is of interest in terms of its capitalization. In the case of whey obtained from donkeys, the amount of protein is very high, thus, it can be successfully used as a substitute in people with allergies to milk of other species and can be harnessed in the form of protein supplements of interest to the population.

A retrospective study on dioxins and dioxin-like polychlorinated biphenyls in milk and dairy products from the Latium region (Italy) over a 7-year study period (2011-2017).

This study reports the data of polychlorinated dibenzo-p-dioxins (PCDDs), -furans (PCDFs), and polychlorinated biphenyls dioxin-like (dl PCBs) measured in a total of 260 samples of the dairy supply chain collected over a period of 7 years (2011–2017) in the Latium region (Italy). Levels and average profiles of congeners were reported for each group of the analyzed dairy matrices, and any differences between different sampling strategies were considered (around likely pollutant sources or casual sampling). Of the samples, 95.4% resulted compliant with the regulated levels; only samples belonging to the “sheep bulk milk” matrix were found to be above either the action levels or the maximum levels (tot. 12 samples). Raw milk of the sheep species showed the highest averages (PCDD/F 0.248 and dl PCB 0.966 WHO TEQ pg/g of fat) compared to the milk of other species. The buffalo milk showed a content of dl PCB significantly lower (dl PCB: 0.371 WHO TEQ pg/g of fat) than the sheep milk (p<0.05). Dioxins were found to be superior to furans in almost all dairy products, except in the noncompliant samples where furans were higher. The OCDD was found to be the most abundant congener in almost all dairy products. This study provides a first list of reference values for background contamination of the dairy supply chain in the Latium region. These pre-existing values will be useful in all cases of environmental pollution to identify critical situations.

Mid-infrared (MIR) spectroscopy for the detection of cow's milk in buffalo milk.

In Italy, buffalo mozzarella is a largely sold and consumed dairy product. The fraudulent adulteration of buffalo milk with cheaper and more available milk of other species is very frequent. In the present study, Fourier transform infrared spectroscopy (FTIR), in combination with multivariate analysis by partial least square (PLS) regression, was applied to quantitatively detect the adulteration of buffalo milk with cow milk by using a fully automatic equipment dedicated to the routine analysis of the milk composition. To enhance the heterogeneity, cow and buffalo bulk milk was collected for a period of over three years from different dairy farms. A total of 119 samples were used for the analysis to generate 17 different concentrations of buffalo-cow milk mixtures. This procedure was used to enhance variability and to properly randomize the trials. The obtained calibration model showed an R2 ≥ 0.99 (R2cal. = 0.99861; root mean square error of cross-validation [RMSEC] = 2.04; R2val. = 0.99803; root mean square error of prediction [RMSEP] = 2.84; root mean square error of cross-validation [RMSECV] = 2.44) suggesting that this method could be successfully applied in the routine analysis of buffalo milk composition, providing rapid screening for possible adulteration with cow’s milk at no additional cost.

Demand for assistance in a human milk bank associated with socioeconomic and maternal-child health factors in Viçosa - Minas Gerais

The superiority of breast milk over milk of other species is already consolidated and there are numerous benefits in the practice of breastfeeding. The Human Milk Bank is a privileged place for the establishment of breastfeeding, as actions to encourage, support and promote breastfeeding are developed. This is a cross-sectional, retrospective study carried out at the only maternity hospital in the city. The sample consisted of mothers and babies assisted by a lactation support program from 2005 to 2016 and also mothers and babies monitored by the human milk bank. It was observed that the assistance of the milk bank was associated with the type of delivery performed (cesarean) (OR: 2.11; 95% CI: 1.03-4.33), having premature children (gestational age) (OR: 3 , 48; 95% CI: 1.52-7.95), higher education (OR: 1.16; 95% CI: 1.03-1.32) and being younger (age) (OR: 0.94; 95% CI %: 0.88-0.99) were the indicators that increased the mother's chance to seek care from the milk bank, regardless of parity and place of residence. The support, protection and promotion of breastfeeding that is carried out in this place, is configured as an important scenario favoring this practice, impacting on the health of the mother-child binomial in the present and in the future.

Recent Insights Into Processing Approaches and Potential Health Benefits of Goat Milk and Its Products: A Review.

Goat milk is considered to be a potential source of various macro- and micro-nutrients. It contains a good proportion of protein, fat, carbohydrates, and other nutritional components which help in promoting nutritional and desirable health benefits. Goat milk is considered to be superior in terms of numerous health benefits, and lower risk of allergy, when compared to the milk of other species. Several processing techniques such as pasteurization, ultrafiltration, microfiltration, and ultrasound have been employed to enhance the quality and shelf life of goat milk and its products. The diverse range of goat milk-based products such as yogurt, cheese, fermented milk, goat milk powder, and others are available in the market and are prepared by the intervention of advanced processing technologies. Goats raised in pasture-based feeding systems are shown to have a better milk nutritional composition than its counterpart. Goat milk contains potential bioactive components, which aids in the maintenance of the proper metabolism and functioning of the human body. This review gives insight into the key nutritional ingredients and bioactive constituents present in goat milk and their potential role in the development of various functional foods using different processing technologies. Goat milk could be considered as a significant option for milk consumption in infants, as compared to other milk available.

Ancient proteins provide evidence of dairy consumption in eastern Africa

Consuming the milk of other species is a unique adaptation of Homo sapiens, with implications for health, birth spacing and evolution. Key questions nonetheless remain regarding the origins of dairying and its relationship to the genetically-determined ability to drink milk into adulthood through lactase persistence (LP). As a major centre of LP diversity, Africa is of significant interest to the evolution of dairying. Here we report proteomic evidence for milk consumption in ancient Africa. Using liquid chromatography tandem mass spectrometry (LC-MS/MS) we identify dairy proteins in human dental calculus from northeastern Africa, directly demonstrating milk consumption at least six millennia ago. Our findings indicate that pastoralist groups were drinking milk as soon as herding spread into eastern Africa, at a time when the genetic adaptation for milk digestion was absent or rare. Our study links LP status in specific ancient individuals with direct evidence for their consumption of dairy products.

Chemometric analysis combined with FTIR spectroscopy of milk and Halloumi cheese samples according to species' origin.

Food adulteration is an issue of major concern, as numerous foodstuffs and beverages do not follow their labeling. Our research interest is in the field of authenticity of dairy products and particularly cheese. Adulteration of dairy products is a well‐known phenomenon, and there are numerous published studies specifically on the authenticity of cheese. In fact, substitution of a portion of fat and/or proteins, adulteration with milk of other species’ origin, and mislabeling of ingredients are some of the main issues that the science of dairy products’ authenticity is regularly facing. Discrimination of dairy products can be determined through several chemical or microbiological methods as presented in the literature. In addition, chemometric analysis is an important tool for interpretation of a huge load of measurements. The aim of this study is to discriminate between various milk samples, which is the primary ingredient of dairy products. Milk samples with different trademarks were analyzed. That data was combined with Halloumi cheese samples for chemometric discrimination of species’ origin. The innovative point of this study is the fact that it is the first time that a research study related to dairy products includes Halloumi cheese which is a traditional Cypriot cheese, not well‐studied until now. The first step of the methodology was the freeze‐drying via lyophilization of the samples. Fourier transformed infrared spectroscopy (FTIR) was chosen for their chemical characterization. Moreover, interpretation of the measurements was carried out by chemometric analysis using SIMCA software. For this study, FTIR data combined with chemometrics have given a very good discrimination of the samples according to their species’ origin. Chemometric methods such as PCA and OPLS‐DA have been used with great success. In the future, this model will be studied regarding geographical origin of the samples.

Animal Colonialism: The Case of Milk

Greta Gaard writes that “[t]he pervasive availability of cows’ milk today—from grocery stores to gas stations—is a historically unprecedented product of industrialization, urbanization, culture, and economics.” To these factors, I would add colonialism and international law; the latter understood broadly to include the rules considered binding between states and nations, transnational law, legal transplants, international food aid, and international trade law. Until the end of the Nineteenth Century, the majority of the world population neither raised animals for their milk nor consumed animal milk. Humans are unique in the mammalian realm in that they drink the milk of other species, including beyond infancy. With the European conquest of the New World and other territories starting in the Sixteenth Century, dairying began to spread worldwide—settlers did not set out to colonize lands and people alone; they brought with them their flora, fauna, and other forms of life, including lactating animals such as cows and sheep.

Modification of the Kjeldahl noncasein nitrogen method to include bovine milk concentrates and milks from other species

The objective of our research was to modify the current indirect casein method for bovine milk to enable it to be applied to bovine milk, bovine milk concentrates, and milks of other species that contain a protein concentration up to 9% (wt/wt). Our work used a series of bovine milk concentrates from about 3 to 9% protein with the same casein as a percentage of true protein to determine the amount of buffer required and pH of the noncasein nitrogen (NCN) filtrate to achieve consistent estimates of casein and casein as percent of true protein. As the concentration of protein in milk increased (either in bovine milk concentrates or in milks of other species), the amount of buffer needed for the NCN sample preparation method to achieve a filtrate pH of 4.6 increased. In the first part of the study using a series of bovine milk concentrates, it was demonstrated that the method gave more consistent predictions of casein as a percentage of true protein when the final NCN filtrate pH was between 4.5 and 4.6 at 38°C. When the amount of buffer added to the sample was not sufficient (i.e., the filtrate pH was too high), the filtrates were not clear. A polynomial equation was developed for prediction of the amount of acetic acid or sodium acetate buffer required to achieve pH 4.5 to 4.6 for milk protein concentrations from 3 to 9% protein using bovine milk and milk concentrates. When the equation developed using cow milk was applied to goat, sheep, and water buffalo milks, it correctly predicted the volume of reagents needed to achieve a final NCN filtrate pH of 4.6 at 38°C. We also verified as part of this work that the ability to measure NPN content of milk was not influenced by protein content of milk in the range from 3 to 9% protein. The results of this study will be used as the basis for proposed changes in the official methods for measurement of the casein content of milk to expand the scope of the method so it can be used to achieve accurate results for milk concentrates and milks of other species.

ELISA for Raw and Heat-Treated Cows and Buffalos Milk in the Milk of Other Species and Sources

Due to the lower prices of cow’s and buffalo’s milk, fraudulent mixing with higher priced milk of other species and sources is economically attractive but illegal and dangerous for allergic consumers. For the rapid detection of cow’s milk, several immunoassays are available but most of them lack the possibility to detect heat-treated cow’s milk due to denaturation of the target protein(s). In the present indirect enzyme-linked immunosorbent assay (ELISA), a mouse monoclonal antibody (Mab) raised against the bovine milk protein A¸-casein is labelled with the enzyme horseradish peroxidase and the binding to the bovine A¸-casein-coated wells of a 96-wells microtiter plate is inhibited by free bovine A¸-casein in the sample or standard. The Mab recognises a 5 amino acids-containing epitope on the glycomacropeptide (GMP) part of bovine A¸-casein which is absent in A¸-casein of goat, sheep, horse, donkey, camel, etc, but is present in A¸-casein of buffalo. Due to the indirect assay format, the cow’s and buffalo’s milk ELISA also recognises heat-treated milk containing denatured A¸-casein. The measurement range of the bovine A¸-casein calibration standards is from 0.1 to 2.5 μg/ml, the limit of detection is 0.05 μg/ml and the only milk sample preparation needed is dilution (100 or 1000 times). This new fast and easy-to-apply ELISA is well suited for the detection of the lower priced raw and heated milk of cows and buffalos in the higher priced milk of other species and sources within the range of 0.25 till 50%.

Review on Production, Quality and Use of Camel Milk in Ethiopia

The camel rearing sub-sector has been an integral part of agriculture in Ethiopia. It has been contributing to the household food, income and poverty alleviation and national economy through export. The country has huge camel resources that made it the sixth leading country in camel population in Africa. However, only little is known about camel or only limited researches have been done on camel. Hence, a little information has been gathered on different aspects of the camel. This is a review of various research results that are from published and unpublished papers over a long period of time and various reviews in the course of the camel and its milk. It has been revealed that the highest percentage of camel population exists in eastern part of the country namely Afar, Somali and eastern part of Oromiya Region. When camels are exposed to more arid and heat area, especially if drinking water is scarce, produce milk that has much higher water content than normal. Thus, it would appear that the lactating camel loses water to the milk in times of drought. This could be a natural adaptation in order to provide not only nutrients, but necessary fluid to the dehydrated camel calf. Pastoralists claim that camel milk is superior to the milk of other species.

Protection Against Hepatitis C and Other Enveloped Viruses? Another Reason Why "Breast Is Best"

Protection Against Hepatitis C and Other Enveloped Viruses? Another Reason Why "Breast Is Best"

Effect of Cameline Nisin isolated from Lactococcus lactis sub sp. lactis on&lt;br&gt;Staphylococcus aureus sp.

The camel milk differs from milk of other species by the presence of a powerful protector system, linked to relatively high levels of lysozyme, lactoperoxidase, lactoferrin, component-3 of proteose peptone (PP3), organic acid, hydrogen peroxide and bacteriocins produced by lactic acid bacteria. The study aims to estimate the part of a bacteriocin (type nisin) produced by a strain of Lactococcus lactis sub sp lactis isolated from camel milk, in this protective system and its antibacterial activity against a strain of Staphylococcus aureus isolated from a bovine milk sample. Optimize conditions for a nisin production, by the producing strain isolated and purified, was carried out, by glucose addition (1%) to the M17 medium. Antagonism tests by the disk method showed inhibition zones (IZ O ) from 6 to 8mm in diameter depending on the incubation time of the producing strain. Thus, for incubation periods of 18h, the production of camel nisin appeared to be maximum (IZ= O 8mm) with a minimum growth of Lactococcus lactis sub sp lactis (pH 6, 37). Optimum growth of camel Lactococcus lactis sub sp lactis strain (pH 6.17) and an optimum production of nisin (IZ= O 8mm) were registered for a 24 hours incubation period.

Macro and micro elements profile of yak (Bos grunniens) milk from Qilian of Qinghai plateau

Twelve elements were analyzed by the ICPAES method for macro (calcium, phosphorus, sulphur, magnesium, potassium, sodium) and micro (cobalt, chromium, copper, iron, zinc, manganese) elements. The macro and micro elements of yak (Qilian) milk were analyzed among different farms and a significant difference was found in phosphorus and sulphur concentrations (P<0.05). Potassium) and sulphur concentrations in yak milk were higher while calcium concentrations were similar to cow milk but lower than milk of other species. Sodium, magnesium and phosphorus concentrations found in this study were in the midrange of those in milk of different animal species. The higher concentrations of potassium and lower concentrations of sodium found in yak milk should be of benefit to patients with hyperpiesia. The composition of yak milk was particularly rich in cobalt, manganese and chromium, while it was deficient in zinc.

Selection of possible marker peptides for the detection of major ruminant milk proteins in food by liquid chromatography-tandem mass spectrometry

The aim of this work was the determination of peptides, which can function as markers for identification of milk allergens in food samples. Emphasis was placed on two casein proteins (α- and β-casein) and two whey proteins (α-lactalbumin and β-lactoglobulin). In silico tryptic digestion provided preliminary information about the expected peptides. After tryptic digestion of four milk allergens, the analytical data obtained by combination of reversed-phase high performance liquid chromatography and quadrupole tandem mass spectrometry (LC-MS/MS) led to the identification of 26 peptides. Seven of these peptides were synthesized and used for calibration of the LC-MS/MS system. Species specificity of the selected peptides was sought by BLAST search. Among the selected peptides, only LIVTQTMK from β-lactoglobulin (m/z 467.6, charge 2+) was found to be cow milk specific and could function as a marker. Two other peptides, FFVAPFPEVFGK from α-casein (m/z 693.3, charge 2+) and GPFPIIV from β-casein (m/z 742.5, charge 1+), occur in water buffalo milk too. The other four peptides appear in the milk of other species also and can be used as markers for ruminant species milk. Using these seven peptides, a multianalyte MS-based method was developed. For the establishment of the method, it was applied at first to different dairy samples, and then to chocolate and blank samples, and the peptides could be determined down to 1 ng/mL in food samples. At the end, spiked samples were measured, where the target peptides could be detected with a high recovery (over 50%).

Breast-feeding multiples

Human breast milk is the best nutrition for human infants. Its advantages over the milk of other species, such as cows, include both a reduced risk for infections, allergies and chronic diseases, together with the full nutritional requirements for growth and development. Breast-feeding is as important for multiples as for singletons. Despite the advantages, multiples receive less breast-feeding than singletons. Common reasons for not breast-feeding multiples include the fear of not fulfilling the infants' needs and the difficulty of coping with the demands on the mother's time. In addition, many multiples are delivered prematurely and by Caesarean section. Maternal pain and discomfort together with anxiety over the infants' condition are not conducive to successful breast-feeding. During lactation, the mother needs to add calories to her daily diet. It has been recommended to add approximately 500–600 kcal/day for each infant. Thus, between eating, nursing and sleeping, life is very busy for the mother of multiples. However, there is evidence that, with appropriate nutrition, one mother can nourish more than one infant. Also, simultaneous breast-feeding can save much time. Combined efforts of parents, close family, friends and the medical team can help to make either full or partial breast-feeding of multiples possible. However, when breast-feeding is not possible, health care workers need to carefully avoid judgmental approaches that may induce feelings of guilt.

Chemical characterisation of the oligosaccharides in hooded seal ( Cystophora cristata) and Australian fur seal ( Arctocephalus pusillus doriferus) milk

Carbohydrates were extracted from hooded seal milk, Crystophora cristata (family Phocidae). Free oligosaccharides were separated by gel filtration and then purified by ion exchange chromatography, gel filtration and preparative thin layer or paper chromatography and their structures determined by 1H-NMR. The hooded seal milk was found to contain inositol and at least nine oligosaccharides, most of which had lacto- N-neotetraose or lacto- N-neohexaose as core units, similar to those in milk of other species of Carnivora such as bears (Ursidae). Their structures were as follows: Gal(β1-4)Glc (lactose); Fuc(α1-2)Gal(β1-4)Glc (2′-fucosyllactose); Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc (lacto- N-neotetraose); Fuc(α1-2)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc (lacto- N-fucopentaose IV); Gal(β1-4)GlcNAc(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(1-4)Glc (lacto- N-neohexaose); Fuc(α1-2)Gal(β1-4)GlcNAc(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (monofucosyl lacto- N-neohexaose a); Gal(β1-4)GlcNAc(β1-3)[Fuc(α1-2)Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (monofucosyl lacto- N-neohexaose b); Fuc(α1-2)Gal(β1-4)GlcNAc(β1-3)[Fuc(α1-2)Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (difucosyl lacto-N-neohexaose); Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc ( para lacto- N-neohexaose); Fuc(α1-2)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc (monofucosyl para lacto- N-neohexaose). Milk of the Australian fur seal, Arctophalus pusillus doriferus (family Otariidae) contained inositol but no lactose or free oligosaccharides. These results, therefore, support the hypothesis that the milk of otariids, unlike that of phocids, contains no free reducing saccharides.

Oligosaccharides in Human Milk and Bacterial Colonization

Oligosaccharides in Human Milk and Bacterial Colonization

Rapid, Sensitive, Enzyme-Immunodotting Assay for Detecting Cow Milk Adulteration in Sheep Milk: A Modern Laboratory Project

Specificity, sensitivity, and experimental simplicity make the immunoenzymatic assay suitable for a variety of laboratories dedicated to diverse activities such as research, quality control in food analysis, or clinical biochemistry. In these assays, the antibody that specifically recognizes the antigen is covalently attached to an enzyme. Once the antigen-antibody immunocomplex is formed, the enzymatic reaction gives a colored product that allows the detection of the initial antigen.The aim of this work was the design of a new laboratory project appropriate for use in courses of biochemistry, immunochemistry, or analytical chemistry. The assay described here detects the presence of cow milk in milk of other species. The main application is the detection of cow milk in sheep milk and cheese. Specific proteins, immunoglobulins (IgG) of the fraudulent bovine milk, are specifically recognized and retained by antibodies immobilized on a membrane. The binding of a second antibody covalently attached to horsera...

Lactose Synthesis in a Monotreme, the Echidna (Tachyglossus aculeatus): Isolation and Amino Acid Sequence of Echidna α-Lactalbumin

α-Lactalbumin and lysozyme were each isolated from echidna (Tachyglossus aculeatus) milk by gel permeation and ion exchange chromatography. The α-lactalbumin modified the action of echidna milk galactosyltransferase to promote the synthesis of lactose but had very little effect on bovine galactosyltransferase. Echidna α-lactalbumin is a glycosylated protein with an apparent molecular weight of 20,000 (SDS-PAGE) whose concentration in the milk is very low compared with the concentrations of α-lactalbumin in the milk of other species. Its amino acid sequence is more similar to that of another monotreme, the platypus (Ornithorhynchus anatinus), than to the sequences of eutherian or marsupial α-lactalbumins. Echidna milk lysozyme, even at high concentrations, did not promote the synthesis of lactose by either echidna or bovine galactosyltransferase. We conclude that lactose synthesis in the echidna occurs by the same mechanism as that found in the platypus and other mammals.