Milk Lactose Content Research Articles

Effects of corn grain endosperm type and fineness of grind on feed intake, feeding behavior, and productive performance of lactating dairy cows

Our objective was to evaluate effects of corn grain endosperm type and fineness of grind on feed intake, feeding behavior, ruminal fermentation, and productive performance of lactating cows. Eight ruminally and duodenally cannulated Holstein cows in mid lactation (130 ± 42 d in milk; mean ± standard deviation) were used in a duplicated 4 × 4 Latin square design with 21-d periods. A 2 × 2 factorial arrangement of treatments was used with main effects of corn grain endosperm type (floury or vitreous) and fineness of grind of corn grain (fine or medium). Rations were formulated to contain 29% starch, 27% neutral detergent fiber, 18.2% forage neutral detergent fiber, and 18% crude protein. Corn grain treatments supplied 86.2% of dietary starch. Endosperm was 25% vitreous for floury corn and 66% vitreous for vitreous corn. Fineness of grind did not affect dry matter intake (DMI), but floury corn tended to reduce DMI (23.8 vs. 25.1 kg/d) compared with vitreous corn. Floury corn increased meal frequency more for fine grind size (9.57 vs. 9.41 meals/d) than medium grind size (9.78 vs. 9.75 meals/d). However, there were no effects of treatment on any other measure of feeding behavior. Endosperm type did not affect yields of milk or milk components or milk composition except that vitreous corn tended to decrease milk lactose concentration compared with floury corn. Finely ground corn decreased yields of milk (31.1 vs. 33.1 kg/d), 3.5% fat-corrected milk (33.1 vs. 35.1 kg/d), milk fat (1.22 vs. 1.32 kg/d), milk lactose (1.48 vs. 1.59 kg/d), and solids not fat (2.46 vs. 2.63 kg/d) compared with medium grind size. However, fineness of grind did not affect milk composition. Treatments had no effect on change in body weight or body condition score or efficiency of milk production (kg of 3.5% fat-corrected milk/kg of DMI). Mean ruminal pH was not affected by treatment, but pH variance was decreased by vitreous compared with floury corn. Total volatile fatty acids and propionate concentrations in the rumen were increased by floury compared with vitreous corn but were not affected by fineness of grind. Effects of fineness of grind on yield of milk and milk components were greater than the effects of corn grain vitreousness.

Dietary supplementation of acetate-conjugated tryptophan alters feed intake, milk yield and composition, blood profile, physiological variables, and heat shock protein gene expression in heat-stressed dairy cows.

The purpose of this study was to investigate the effects of dietary supplementation of rumen-protected tryptophan (RPT) at four levels on milk yield, milk composition, blood profile, physiological variables, and heat shock protein gene expression in dairy cows under conditions of moderate-severe heat stress (MSHS, THI=80~89). Sixteen early-lactating dairy cows (body weight=719±66.4kg, days in milk=74.3±7.1, milk yield=33.55±3.74kg, means±SEM) were randomly assigned in a factorial arrangement to one of the four treatments: control group (n=4, no RPT supplementation), 15g/d RPT (n=4), 30g/d RPT (n=4), or 60g/d RPT group per cow (n=4) supplemented to the TMR. A higher dry matter intake (DMI) and milk yield were found in the 30g RPT group compared with the other groups, and the 3.5% fat-corrected milk yield, energy-corrected milk yield, milk fat, protein, β-casein, mono-unsaturated fatty acid, and poly-unsaturated fatty acid contents, and serum glucose content were observed in the 30g RPT group (p<0.05). The milk lactose concentration was significantly higher in the 30g RPT group compared with the control and 60g RPT groups (p<0.05). The plasma cortisol level was lower, while the serotonin and melatonin concentrations were higher in the 30g group compared with the other groups (p<0.05). Heat shock protein (HSP) 70 expression was downregulated in the control and 15g RPT groups, whereas the expression of HSP90 and HSPB1 remained unchanged among the groups. In particular, the 30g RPT group was considered to have an improved DMI, milk yield, and lactose concentration, as well as anti-heat stress effects due to the simulation of serotonin and melatonin during MSHS.

Effect of different feeding regimes on productive performances of HF × Kankrej crossbred cows

Present experiment was conducted to study the effect of different feeding regimes on productive performances of HF × Kankrej crossbred cows. The study was conducted on 18 crossbred cows which were distributed into three treatment groups comprising of 6 animals each. Animals of T1 (Farmers’ feeding) group were maintained as per the feeding regime followed by small and marginal farmers in India. Animals of T2 (Modified feeding) group comprised of feeding with scientific interventions. Animals of T3 (Farm feeding) group were fed as per feeding practices followed at Livestock Research Station, Anand Agricultural University. Milk yield and 4% Fat Corrected Milk (FCM) yield was significantly higher in T2 and T3 as compared to T1 group. Lactation yield was highest in T3 followed by T2 and T1 groups. Average total solid of milk was significantly more in T3 as compared to T1 and T2 groups. Milk fat was maximum in T3 followed by T2 and T1 groups which did not differ significantly among each other. SNF, protein and lactose content of milk was found significantly higher in T3 as compared to T1 and T2 groups. Milk salt was highest in T3 followed by T2 and T1 group which differed significantly among each other. It may be concluded from the present study that feeding regime significantly influenced productive performances of crossbred cows as modified feeding group performed better over farmers feeding group and at par with farm feeding group.

Lactose: Use, measurement, and expression of results

Lactose has different uses in the dairy, food, and pharmaceutical industries. Being aware of the different forms of lactose and their concentrations can be very helpful in managing dairy product quality, properties, and manufacturing efficiency. Correct measurement and reporting of lactose concentration in milk and other dairy products will be of increased importance in the future as more value-added uses of lactose are developed and as milk lactose data are used in farm management decision making. Lactose should be reported as anhydrous lactose because lactose data will be used to make increasingly important decisions in dairy processing, dairy product labeling, and milk production in the future. Lactose also plays an important role in milk synthesis within a cow. Milk production factors and dairy cattle breed selection influence the amount of high value fat and protein produced per unit of lactose. If the off-farm value of lactose remains low, more attention may be focused on using ultrafiltration to process milk and leave 50 to 60% of the lactose and water from milk at the farm to recover the energy value of the lactose as feed and reduce the hauling cost of the high value components of milk to a dairy product manufacturing factory. Many methods exist to determine lactose concentration, but the most important methods are enzymatic assays, HPLC, and mid-infrared analysis. New, value-added uses for lactose need to be developed. Consistent and accurate methods of lactose measurement and consistent expression of lactose results will support this development process. Starting in January 2017, the USDA Federal Milk Market Laboratories began reporting lactose content of milk as anhydrous lactose and discontinued the reporting of lactose by difference.

Coagulation process in Manchega sheep milk from Spain: A path analysis approach

Characteristics of sheep milk are of great interest for the dairy industry, as almost the totality of production is intended for cheesemaking. However, the existing relationships between these variables are complex. This study assessed composition, hygienic quality, coagulation properties, and curd yield of 1,200 individual Manchega sheep milk samples. The aim was to compare the effect of composition and hygienic quality on coagulation and curdling, and to evaluate the relationship between curd yields and the coagulation process and the effect of other features by using path analysis methodologies. Outcomes proved path analysis to be a useful and effective tool to assess these relationships through direct and indirect paths within the same model. Results showed that the factors that had a direct influence on milk coagulation were lactose concentration, casein content, and initial pH of milk. Contrastingly, somatic cells did not seem to have any effect (direct or indirect) on the coagulation process. Factors that directly affected curd yield were fat content, lactose concentration, casein content, and curd moisture. However, technological parameters showed little effect over curd yield.

Comparison of yield, composition and quality of milk of Polish Holstein-Friesian cows in conventional and automatic milking systems

Abstract The aim of the present study was to evaluate the changes in selected production and functional traits of Polish Holstein-Friesian cows after switching from a conventional (CMS) to an automatic milking system (AMS). The study consisted of 3398 Polish Holstein- Friesian dairy cows, from 16 herds in which CMS was changed to AMS. Cows were in their 1st (L1) or 2nd lactation (L2). The data consisted of milk yield [MY, kg], fat content [FC, %], protein content [PC, %], dry matter [DM, %], lactose content [LC, %], urea content [MU, mg/l], somatic cell count [SCC, thous./ml] and score [SCS, log]. The milking system had a significant impact on milk yield, fat, lactose, dry matter and urea contents. Regardless of lactation number, milk derived from CMS was characterised by higher values for FC, PC, DM SCC and SCS, while milk from AMS had higher MY, LC and MU. Multifactor analysis of variance also confirmed significant effect of herd, season, herd × milking system interaction on SCS in milk of cows in L1. In the studied herds change from CMS to AMS was evaluated separately for cows in L1 and L2. The transitioning from CMS to AMS resulted in the decrease of fat content in 6 L1 and 7 L2 herds, dry matter in 8 L1 and 5 L2 herds. SCS in milk also decreased in 4 L1 and 5 L2 herds. The change caused the increase of MY in 11 L1 and 9 L2 herds, lactose content in 6 L1 and 4 L2 herds and urea content in 9 L1 and 10 L2 herds. AMS may positively affect milk yield and health status, however, the change of milking system should be also accompanied by the change in herd management.

Effect of total replacement of inorganic with organic sources of key trace minerals on performance and health of high producing dairy cows

The aim of this trial was to compare the effect of complete replacement of certain inorganic trace minerals with organic, chelated minerals (Bioplex®, Selplex®; Alltech Inc, USA) against standard and reduced inorganic mineral levels in feed on the performance and health of dairy cows during a 10-week trial (W0-W10) period. Three treatment groups, each containing 36 dairy cows, were assigned to one of three diets that varied only in mineral feed composition: (1) Inorganic (CON) Cu (1,042 mg/kg), Se (26 mg/kg) and Zn (4,200 mg/kg) as per normal industry inclusion levels; (2) Inorganic (INORG) Cu (-35%: 680 mg/kg), Se (-60%; 10 mg/kg) and Zn (-66%; 1,360 mg/kg) (based on the lower organic levels in diet 3); and (3) Organic (proteinates and selenium-enriched yeast) minerals (ORG) Cu, Se and Zn as per INORG levels. The cows in the ORG group had the highest milk yield (32.6 kg; P=0.0165) with the INORG group the least (31.3 kg), production (energy corrected milk 31.5 kg (P=0.008) vs CON 31.0 kg and INORG 30.7 kg), protein synthesis (ORG 1,024 g/d (P&lt;0.0001) vs CON 1,013 g/d and INORG 982 g/d) and ORG had the highest (P&lt;0.01) milk lactose content. There were no differences between treatments in plasma Zn, Cu and GSH-PXE levels. Pregnancy rate and number of artificial insemination services appeared improved. Mastitis rates were lowest for the ORG group (two cows affected), vs four cows for the INORG group and six cows for the CON group. Somatic cell counts were consistently lower in the ORG cows (P&lt;0.05), being less than 300,000 cell counts, compared to peaks of 380,000 counts in the INORG and 550,000 counts in the CON groups. Five cows were affected by lameness in the INORG group, compared to three for the CON and one in the ORG groups.

Non-genetic effects on daily milk yield and components of Holstein cows in Morocco

The objectives of this study were to determine milk yield and constituents' levels of Holstein cows under Moroccan conditions and to assess the effects of environmental factors influencing them. Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS). Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively. The effects of herd and calving year were found to be significant on all the studied traits. Parity was determined to have significant effects on all analyzed traits, with the exception of MUN concentration. Milk yield, fat content, protein content, and SCS increased with parity, whereas lactose percentage decreased in later parities. Except fat and lactose contents, all the other studied variables were significantly influenced by calving season. Milk yield and MUN were highest for cows calving during humid season, whereas protein content, SNF content, and SCS tended to be higher in dry season. All the studied traits changed significantly according to stage of lactation. The peaks of milk yield and lactose content occurred between 35 and 65 days, whereas that of MUN between 95 and 125 days after parturition. Protein and SNF contents and SCS attained their lowest values between days 35 and 65, while fat content between days 65 and 95. It was concluded that these environmental factors should be taken into consideration if the milk yield and quality in Morocco is to be optimized.

Determination of Lactose Concentration in Low-Lactose and Lactose-Free Milk, Milk Products, and Products Containing Dairy Ingredients, Enzymatic Method: Single-Laboratory Validation First Action Method 2020.08.

BackgroundThe AOAC Stakeholder Panel on Strategic Food Analytical Methods issued a call for methods for the measurement of lactose in low-lactose and lactose-free products under Standard Method Performance Requirement (SMPR®) 2018.009. Megazyme’s Lactose Assay Kit (K-LOLAC) was developed specifically to address the need for accurate enzymatic testing in lactose-free samples.ObjectiveK-LOLAC was validated for measurement of lactose in low-lactose and lactose-free milk, milk products, and products containing dairy ingredients. A single-laboratory validation (SLV) of the method is reported.MethodK-LOLAC is an accurate and sensitive enzymatic method for the rapid measurement of lactose in low-lactose or lactose-free products. Validation analysis was performed on a sample set of 36 commercial food and beverage products and a set of 10 certified reference materials. Parameters examined during the validation included working range and linear range, selectivity, LOD, LOQ, trueness (bias), precision (repeatability and intermediate precision), robustness, and stability.ResultsFor all samples tested within the lower range (10–100 mg/100 g or mL), recoveries varied from 93.21–114.10%. Recoveries obtained for samples in the higher range (>100 mg/100 g or mL) varied from 94.44–108.28%. All materials had repeatability relative standard deviations (RSDr and RSDir) of <9%.ConclusionsThe commercial K-LOLAC assay kit, as developed by Megazyme, meets the requirements set out under SMPR 2018.009.HighlightsK-LOLAC is a robust, quick, and easy method for analysis of lactose in foodstuffs and beverages.

Influence of Calving Ease on In-Line Milk Lactose and Other Milk Components.

Simple SummaryCalving is a difficult moment in a cow’s life that causes stress, and the ease of calving determines the course of further lactation. The hypothesis of our study was to investigate how the difficulty of calving may influence changes in lactose concentration and other milk components and how well these two factors correlate between each other. We found a statistically significant (p < 0.001) negative correlation of calving ease score with milk lactose % (r = −0.376) and positive correlation coefficients with milk lactose yield (kg) (r = 0.277) as well as milk fat/lactose % ratio (r = 0.191). The analysis showed a regular increase (p < 0.001) with decreasing calving ease scores for milk electric conductivity and milk somatic cell count.The aim of our study was to determine how the ease of calving of cows may influence changes in lactose concentration and other milk components and whether these two factors correlate with each other. To achieve this, we compared data of calving ease scores and average percentage of in-line registered milk lactose and other milk components. A total of 4723 dairy cows from nine dairy farms were studied. The cows were from the second to the fourth lactation. All cows were classified according to the calving ease: group 1 (score 1)—no problems; group 2 (score 2)—slight problems; group 3 (score 3)—needed assistance; group 4 (score 4)—considerable force or extreme difficulty. Based on the data from the milking robots, during complete lactation we recorded milk indicators: milk yield MY (kg/day), milk fat (MF), milk protein (MP), lactose (ML), milk fat/lactose ratio (MF/ML), milk protein/lactose ratio (MP/ML), milk urea (MU), and milk electrical conductivity (EC) of all quarters of the udder. According to the results, we found that cows that had no calving difficulties, also had higher milk lactose concentration. ML > 4.7% was found in 58.8% of cows without calving problems. Cows with more severe calving problems had higher risk of mastitis (SCC and EC). Our data indicates that more productive cows have more calving problems compared to less productive ones.

Milk Yield and Composition of Mixed-Breed Goats on Rangeland during the Dry Season and the Effect on the Growth of Their Progeny.

Simple SummaryWe tested whether the milk yield capacity and composition of mixed-breed goats on rangeland in northern Mexico during the dry season affects body weight gain and weaning weight of their progeny. Maternal body weight positively influenced milk yield and milk components (p < 0.05 to p < 0.001). Lactose and solids-non-fat content of milk differed (p < 0.05) between goats with different milk yield capacity, whereas milk protein content tended to differ (p = 0.08) and milk fat content did not (p > 0.05). Milk yield and composition throughout 105-d lactation did not influence body weight gain and weaning weight of the offspring.We tested whether the milk yield capacity of mixed-breed goats on a Chihuahuan desert rangeland in northern Mexico during the dry season affects milk composition, body weight gain, and weaning weight of their progeny. Milk yield and composition, and progeny postnatal growth performance, were recorded weekly. One week after kidding, mixed-breed goats (a mixture of Criollo × dairy breeds; n = 40) were allotted into medium (MP) or low (LP) milk yielding groups (20 goats per group). Mean 105-d total milk yield for MP and LP goats was 45.2 ± 12.5 and 20.7 ± 5.2 L, respectively. Milk lactose (4.3 vs. 4.2%) and solids-non-fat (SNF; 8.2 vs. 8.0) differed (p < 0.05) between MP and LP goats; milk protein content tended to differ (p = 0.08) between MP and LP goats with no difference for milk fat content (p > 0.05). Maternal body weight was positively associated with milk yield, milk lactose, and SNF content (p < 0.05 to p < 0.001). Goats giving birth to males produce more milk than goats giving birth to females, but milk fat percentage was higher in goats bearing females (p < 0.001). Milk yield and composition throughout lactation did not influence body weight gain (47.8 vs. 48.7 g/day for kids from MP and LP goats) and weaning weight (6.7 vs. 6.7 kg from MP and LP goats) of the offspring (p > 0.05). Birth weight and weaning weight of the progeny were positively related to maternal body weight (p ≤ 0.05). The postnatal growth of the kids was reduced, extending the time to reach market weight. Nevertheless, non-supplemented mixed-breed goats reared on semi-arid rangeland of northern Mexico have the potential for moderate milk production. Therefore, due to the limited nutrients ingested by grazing goats during the dry season, a nutritional supplement is necessary to keep up milk production and adequate growth of kids.

Effect of protein level and methionine supplementation on dairy cows during the transition period

Cows experience a significant negative protein balance during the first 30 d of lactation. Given the functional effects of AA on health, especially in challenging periods such as calving, higher levels of protein and specific AA in the diet may act to improve health and feed intake. The response of dairy cows to 3 protein supplementation strategies during the transition period and through the first 45 d in milk was evaluated. The final data set had 39 Holstein cows blocked based on parity (primiparous vs. multiparous) and expected calving and randomly assigned within each block to one of 3 dietary treatments: low protein (LP), high protein (HP), or high protein plus rumen-protected methionine (HPM). Treatments were offered from d -18 ± 5 to 45 d relative to parturition. Pre- and postpartum diets were formulated for high metabolizable protein (MP) supply from soybean meal, and HP and HPM provided higher MP balance than LP. Preplanned contrasts were LP versus HP+HPM and HP versus HPM. Significance was declared at P ≤ 0.05 and trends at 0.05 <P ≤ 0.10. Cows fed HP and HPM had greater fry matter intake (DMI) prepartum than LP (+2 kg/d), and there was a trend for greater DMI with HPM than with HP (+1.6 kg/d). Body weight and condition score before and after calving did not differ among treatments. High protein (HP and HPM) tended to increase milk yield during the first 45 d of lactation (+1.75 kg/d), increased milk lactose content and urea-N in milk and plasma, tended to increase blood BHB 14 d postpartum, and tended to reduce milk/DMI compared with LP. Blood concentrations of calcium at calving and of glucose, and nonesterified fatty acids pre- and postpartum did not differ. High protein induced lower concentration of plasma IL-1 at calving and lowered blood lymphocytes 21 d postpartum, suggestive of a reduced inflammatory status compared with LP. The concentrations of IL-10, tumor necrosis factor alpha, and other hemogram variables did not differ among treatments. Addition of rumen-protected methionine to the HP diet did not alter milk yield but increased fat and total solids concentrations. The rumen-protected methionine had no effect on blood metabolites and immunity markers, with the exception of increased pre-partum insulin concentrations. The data indicate that dairy cows around calving respond positively to an increase in the supply of MP and to rumen-protected methionine supplementation of the HP diet by increasing intake and improving immune status.

Fava beans can substitute soybean meal and rapeseed meal as protein source in diets for lactating dairy cows

The effect of replacing mixtures of wheat and soybean meal and wheat and rapeseed meal by toasted fava beans, and the effect of toasting fava beans on feed intake, milk yield, and composition of milk and feces were investigated using 40 Holstein cows in each of two 4 × 4 Latin square design trials conducted simultaneously. In trial 1, the 4 treatment concentrates were untreated fava beans, toasted fava beans, 42% soybean meal + 58% rolled wheat, and a 21, 29, and 50% mix of soybean meal, rolled wheat, and toasted fava beans, respectively [on dry matter (DM) basis]. In trial 2, the 4 experimental treatments were untreated fava beans, toasted fava beans, 64% rapeseed meal + 36% rolled wheat, and a 32, 18, and 50% mix of rapeseed meal, rolled wheat, and toasted fava beans, respectively (on DM basis). In each trial, 16 primiparous and 24 multiparous cows were fed the treatment concentrates as part of a partial mixed ration, of which the forage consisted of 50% corn silage and 50% grass-clover silage. Substitution of soybean meal and wheat or rapeseed meal and wheat with toasted fava beans did not affect total DM intake, and no linear effects were observed on milk yield or energy-corrected milk (ECM) yield. However, in trial 2, a quadratic effect was observed on milk yield when substituting rapeseed meal and wheat with toasted fava beans. In both trials, substitution of soybean meal and wheat or rapeseed meal and wheat with toasted fava beans increased milk lactose concentration and decreased milk protein yield and concentration of protein in milk. In both trials, fecal concentration of starch increased linearly when substituting soybean meal and wheat or rapeseed meal and wheat with toasted fava beans. In trial 2, fecal concentration of P decreased when substituting rapeseed meal and wheat with toasted fava beans. In situ investigations showed increased rumen undegradable protein concentration and thereby increased estimated metabolizable protein supply when toasting fava beans. However, in both trials, milk protein yield and concentration decreased when cows were fed toasted compared with untreated fava beans. Furthermore, when cows were fed toasted compared with untreated fava beans in trial 1, milk yield, ECM yield, and nitrogen efficiency decreased. We conclude that toasted fava beans could substitute soybean meal and wheat or rapeseed meal and wheat with regard to ECM yield. However, milk protein yield decreased when substituting soybean meal and wheat or rapeseed meal and wheat with toasted fava beans. Compared with untreated fava beans, toasting had no positive effect on milk production and nitrogen efficiency.

Acute changes to breast milk composition following consumption of high-fat and high-sugar meals.

Breast milk composition is influenced by habitual diet, yet little is known about the short‐term effects of changes in maternal diet on breast milk macronutrient concentrations. Our aim was to determine the acute effect of increased consumption of sugar/fat on breast milk protein, lactose and lipids. Exclusively breastfeeding women (n = 9) were provided with a control, higher fat (+28 g fat) and higher sugar (+66 g sugar) diet over three separate days at least 1 week apart. Hourly breast milk samples were collected concurrently for the analysis of triglycerides, cholesterol, protein, and lactose concentrations. Breast milk triglycerides increased significantly following both the higher fat and sugar diet with a greater response to the higher sugar compared to control diet (mean differences of 3.05 g/dL ± 0.39 and 13.8 g/dL ± 0.39 in higher fat and sugar diets, respectively [P < 0.001]). Breast milk cholesterol concentrations increased most in response to the higher sugar diet (0.07 g/dL ± 0.005) compared to the control (0.04 g/dL) and the higher fat diet (0.05 g/dL) P < 0.005. Breast milk triglyceride and lactose concentrations increased (P < 0.001, P = 0.006), whereas protein decreased (p = 0.05) in response to the higher fat diet compared to the control. Independent of diet, there were significant variations in breast milk composition over the day; triglycerides and cholesterol concentrations were higher at end of day (P < 0.001), whereas protein and lactose concentrations peaked at Hour 10 (of 12) (P < 0.001). In conclusion, controlled short‐term feeding to increase daily sugar/fat consumption altered breast milk triglycerides, cholesterol, protein and lactose. The variations observed in breast milk protein and lactose across the 12 h period is suggestive of a circadian rhythm.

Effect of substituting wet corn gluten feed and corn stover for alfalfa hay in total mixed ration silage on lactation performance in dairy cows

Wet corn gluten feed (WCGF) is a high moisture feed containing rapidly digestible, non-forage fiber and protein. The objective of this study was to investigate the effect of substituting WCGF and corn stover for alfalfa hay in total mixed ration (TMR) silage on lactation performance and nitrogen balance in dairy cows. Nine multiparous Holstein dairy cows (BW = 532 ± 28.9 kg and day in milk = 136 ± 5.6 d; mean ± SD) were used in a replicated 3 × 3 Latin square design with 21-d periods (14 d of diet adaption and 7 d of sample collection). Groups were balanced for parity, day in milk, and milk production and consumed one of three treatment diets during each period. The treatment diets were fed as TMR and contained similar concentrate mixtures and corn silage but different proportions of roughage and WCGF. The three treatments were: (1) 0% WCGF, 0% corn stover, and 22.1% alfalfa hay (0% WCGF); (2) 6.9% WCGF, 3.4% corn stover, and 11.8% alfalfa hay (7% WCGF); and (3) 13.3% WCGF, 4.9% corn stover, and 3.9% alfalfa hay (13.3% WCGF). Compared to the 0% WCGF diet, the cows fed the 7% and 13.3% WCGF diets had a higher milk yield and concentration of milk fat, protein, lactose, and total solids. Effective degradability of DM was higher in the cows fed the 7% and 13.3% WCGF diets than it was with the 0% WCGF diet. Cows fed the 13.3% WCGF had a higher CP effective degradability and a lower rumen undegraded protein than cows fed the 0% WCGF diet. The concentration of ruminal volatile fatty acids and ammonia-N was higher in cows fed the 7% and 13.3% WCGF diets than cows fed the 0% WCGF diet. The fecal N was lower in cows fed the 7% and 13.3% WCGF diets than it was in cows fed the 0% WCGF diet. Milk N secretion and milk N as a percent of N intake were higher in cows fed the 13.3% WCGF diet than cows fed the 0% and 7% WCGF diets. In conclusion, it appears that feeding a TMR silage containing WCGF and corn stover in combination, replacing a portion of alfalfa hay, may improve lactation performance and nitrogen utilization for lactating dairy cows.

The evaluation of the relationship of lactose to production and reproduction traits in different breeding conditions of the Slovak Spotted dairy cows

The evaluation of the relationship of lactose to production and reproduction traits in different breeding conditions of the Slovak Spotted dairy cows

Effect of High-Pressure Processing (HPP) on composition, lactose and microstructure of goat milk

Thermal pasteurisation is an established method for milk processing. However, the high temperature could affect the micronutrients in the milk. High pressure processing (HPP) is a cold alternative to thermal pasteurisation that can maintain the fresh-like properties of liquid food. However, employing pressure could potentially affect the composition and microstructure of milk and milk products. Therefore, this study focusses on evaluating the effect of high pressure processing (HPP) towards the composition, lactose content and microstructure (in term of fat globules) of goat milk. The goat milk was subjected to HPP at a pressure range of 200 to 600 MPa and process holding time at 5 - 15 mins. There were insignificant differences in terms of fat, protein and carbohydrate, but significant changes observed for lactose content of pressurised goat milk (PGM). The lactose content of PGM was in the range of (2.540 – 2.986 g/mL), while 1.253±0.01 g/100 mL for untreated goat milk (UGM). A higher number of the small size of goat milk fat globules observed at 600 MPa compared to lower processing pressure (200 and 400 MPa) at the same pressure holding time (5 to 15 mins). The mean diameters of fat globules were in the range of 5.215 to 5.651 μm. This size reduction of milk fat globules is an advantage for cheese making or other dairy product making industries, because it can help to possess a smoother and more refined texture of milk products.

Effect of Soybean Oil Supplementation on Milk Production, Digestibility, and Metabolism in Dairy Goats under Thermoneutral and Heat Stress Conditions.

Simple SummaryHeat stress (HS) not only reduces milk yield but also depresses its contents of fat and protein, which might negatively impact cheese making. Dietary supplementation with soybean oil (SBO) could increase milk fat and improve milk fatty acid (FA) profiles in dairy goats. In the present study dairy goats were exposed to thermoneutral (TN; 15 to 20 °C) or HS (12 h/d at 37 °C and 12 h/d at 30 °C) conditions. In each ambient temperature, goats were fed a control diet (CON) or the same diet supplemented with SBO. Goats in HS suffered depressed feed intake and milk production, but they had greater digestibility coefficients compared to TN goats. Regardless of the HS treatment, goats supplemented with SBO produced milk with greater contents of fat, monounsaturated FA, and conjugated linoleic acid, without any negative effects on milk protein content. In conclusion, dietary supplementation with soybean oil was a useful strategy to increase milk fat and improve its fatty acid profile. Both TN and HS goats responded to soybean oil supplementation similarly since the interaction between soybean oil supplementation and temperature treatment was not significant.In a previous work, we observed that heat-stressed goats suffer reductions in milk yield and its contents of fat and protein. Supplementation with soybean oil (SBO) may be a useful strategy to enhance milk quality. In total, eight multiparous Murciano–Granadina dairy goats (42.8 ± 1.3 kg body weight; 99 ± 1 days of lactation) were used in a replicated 4 × 4 Latin square design with four periods; 21 d each (14 d adaptation, 5 d for measurements and 2 d transition between periods). Goats were allocated to one of four treatments in a 2 × 2 factorial arrangement. Factors were no oil (CON) or 4% of soybean oil (SBO), and controlled thermal neutral (TN; 15 to 20 °C) or heat stress (HS; 12 h/d at 37 °C and 12 h/d at 30 °C) conditions. This resulted in four treatment combinations: TN-CON, TN-SBO, HS-CON, and HS-SBO. Compared to TN, HS goats experienced lower (p < 0.05) feed intake, body weight, N retention, milk yield, and milk protein and lactose contents. However, goats in HS conditions had greater (p < 0.05) digestibility coefficients (+5.1, +5.2, +4.6, +7.0, and +8.9 points for dry matter, organic matter, crude protein, neutral detergent fiber, and acid detergent fiber, respectively) than TN goats. The response to SBO had the same magnitude in TN and HS conditions. Supplementation with SBO had no effects on feed intake, milk yield, or milk protein content. However, SBO supplementation increased (p < 0.05) blood non-esterified fatty acids by 50%, milk fat by 29%, and conjugated linoleic acid by 360%. In conclusion, feeding 4% SBO to dairy goats was a useful strategy to increase milk fat and conjugated linoleic acid without any negative effects on intake, milk yield, or milk protein content. These beneficial effects were obtained regardless goats were in TN or HS conditions.

Abomasal infusion of ground corn and ammonium chloride in early-lactating Holstein-Friesian dairy cows to induce hindgut and metabolic acidosis

Next to rumen acidosis, other forms of acidosis may also affect lactational performance of cows. Therefore, the effects of hindgut acidosis, induced via abomasal infusion of ground corn, and metabolic acidosis, induced via abomasal infusion of NH4Cl, were studied in cows in early lactation. Observations were made on intake and digestibility of nutrients, lactation performance, energy and N partitioning, blood acid-base status, and rumen and hindgut fermentation characteristics. In a 6 × 6 Latin square design, 6 rumen-fistulated, second-lactation Holstein-Friesian dairy cows (48 ± 17 d in milk) were subjected to 5 d of continuous abomasal infusions of water as control, or solutions of 2.5 mol of NH4Cl/d, 5.0 mol of NH4Cl/d, 3.0 kg of ground corn/d, or the combination of ground corn with either of the 2 NH4Cl levels, followed by 2 d of rest. Treatment solutions were administered via peristaltic pumps through infusion lines attached to the rumen cannula plug and an abomasal infusion line with a flexible disk (equipped with holes to allow digesta passage) to secure its placement through the sulcus omasi. A total mixed ration consisting of 70% grass silage and 30% concentrate (on dry matter basis) was fed at 95% of ad libitum intake of individual cows. The experiment was conducted in climate respiration chambers to determine feed intake, lactation performance, and energy and N balance. Abomasal infusion of NH4Cl affected the acid-base status of the cows, but more strongly when in combination with abomasal infusion of ground corn. Metabolic acidosis (defined as a blood pH < 7.40, blood HCO3 concentration < 25.0 mmol/L, and a negative base excess) was observed with 5.0 mol of NH4Cl/d, 3.0 kg of ground corn/d + 2.5 mol of NH4Cl/d, and 3.0 kg of ground corn/d + 5.0 mol of NH4Cl/d. Metabolic acidosis was associated with decreased milk lactose content, metabolic body weight, energy retained as protein, and fecal N excretion, and increased urine N excretion, and tended to decrease intake of nutrients. Digestibility of several nutrients increased with 5.0 mol of NH4Cl/d, likely as a result of decreased intake. Abomasal ground corn infusion resulted in hindgut acidosis, where fecal pH decreased from 6.86 without ground corn to 6.00 with ground corn, regardless of NH4Cl level. The decrease in fecal pH was likely the result of increased hindgut fermentation, evidenced by increased fecal volatile fatty acid concentrations. Hindgut acidosis was associated with decreased digestibility of nutrients, except for starch, which increased, and crude fat, which was not affected. No systemic inflammatory response was observed, suggesting that the hindgut epithelium was not severely affected by the more acidic conditions or barrier damage. Abomasal infusion of ground corn increased milk yield, milk protein and lactose yield, fecal N excretion, N use efficiency, and total energy retained as well as energy retained in fat, and reduced milk fat content and urine N excretion.

Effects of bamboo leaf extract on the production performance, rumen fermentation parameters, and rumen bacterial communities of heat-stressed dairy cows.

ObjectiveAn experiment was conducted to evaluate the effects of bamboo leaf extract (BLE) on the production performance, rumen fermentation parameters, and rumen bacterial communities of heat-stressed dairy cows.MethodsThe experiment comprised a 14-day adaptation period and a 21-day experimental period and was conducted in a high-temperature and humidity environment (daily mean ambient temperature = 33.5°C±1.3°C; daily mean relative humidity = 64.9%±0.8%, daily mean temperature-humidity index = 86.2±0.4). Twelve Holstein dairy cows were randomly allocated into two groups. A total mixed ration supplemented with BLE at 0 (CON) and 1.3 g/kg dry matter (DM) were fed, respectively. Feed intake and milk yield were recorded daily. Milk samples were collected on 1, 11, and 21 d of the experimental period to analyze milk performance. Rumen fluid samples were collected on 21 d of the experimental period to analyze rumen fermentation parameters and rumen bacterial communities.ResultsCompared with the control group, supplementation of BLE increased milk yield (p<0.01), milk fat yield (p = 0.04), 4% fat-corrected milk (p<0.01) and milk fat content (p<0.01); reduced somatic cell count (p<0.01). No differences in DM intake and milk protein or lactose content were observed between two groups. Supplementation of BLE also increased the rumen total volatile fatty acid (p<0.01), acetate (p<0.01), butyrate (p<0.01), and valerate (p = 0.05) concentrations. However, no significant effects were observed on rumen pH, ammonia nitrogen, propionate, acetate/propionate ratio, isobutyrate, or isovalerate. Furthermore, BLE increased the rumen bacterial abundance and the diversity of the rumen bacterial community. The BLE reduced the Firmicutes/Bacteroidetes abundance ratio and increased the abundances of Butyrivibrio_2 (p<0.01) and Ruminococcus_2 (p<0.01).ConclusionThe BLE supplementation at 1.3 g/kg DM could improve production performance and rumen fermentation in dairy cows during heat stress.