Mild Heat Stress Research Articles

Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants

Potato is a globally important, highly heat-susceptible crop species. We investigated the effects of prolonged exposure to elevated temperatures and exogenous salicylic acid (SA) on microplant growth and heat-shock response (HSR) in three unrelated potato genotypes/cultivars. Long-term exposure to 29 °C (mild heat stress) caused a significant reduction in the number of surviving explants and shoot morphometric parameters in heat-sensitive genotypes, while exposure to 26 °C (warming) caused only a decline in shoot growth. Interestingly, 26 °C-temperature treatment stimulated root growth in some investigated genotypes, indicating a difference between favorable temperatures for potato shoot and root growth. SA showed a protective effect regarding potato shoot growth at 26 °C. At 29 °C, this effect was genotype-dependent. SA did not affect the number of roots and inhibited root elongation at all temperature treatments, indicating the difference between shoot and root responses to applied SA concentration. Although HSR is mainly considered rapid and short-lived, elevated transcript levels of most investigated HSFs and HSPs were detected after three weeks of heat stress. Besides, two StHSFs and StHSP21 showed elevated expression at 26 °C, indicating extreme potato heat-susceptibility and significance of HSR during prolonged warming. SA effects on HSFs and HSPs expression were minor and alterable.

Preexposure to heat stress attenuates sepsis-associated inflammation and cognitive decline in rats

Sepsis is a life-threatening organ dysfunction due to an infection, leading to cognitive impairments. Studies have shown that heat shock protein 70 (HSP70) exhibited a neuroprotective effect. In this study we used mild heat stress to induce expression of HSP70, aiming to detect the effect of HSP70 on neurocognitive deficits associated with sepsis and explored the underlying mechanisms. Male rats were exposed to 42℃ for 15 min. After 12 h, they were subjected to cecal ligation and puncture (CLP). HSP70 and brain-derived neurotrophic factor (BDNF) expression, and nuclear level of NF-κB p65 were detected by western blot. Levels of inflammatory cytokines in circulation and hippocampus were measured by ELISA and RT-PCR. Neuronal morphology and damage of hippocampal neurons were assessed by Hematoxylin-Eosin (HE) and Nissl stainings. Microglial activation was determined by immunohistochemistry. Finally, neurologic and cognitive functions were evaluated using neurobehavioral scoring and morris water maze (MWM) test. Mild heat stress increased survival rate of sepsis rats. Mild heat stress upregulated HSP70, inhibited nuclear level of NF-κB p65 in hippocampus. Mild heat stress could diminish IL-1β and TNF-α levels in circulation and hippocampus. Furthermore, mild heat stress was able to enhance expression of BDNF and alleviate cognitive impairment after sepsis. Overall, these results indicated that mild heat stress showed protective effects on sepsis-associated encephalopathy rat model, which may be associated with upregulation of HSP70 and inhibition of NF-κB pathway.

Heat stress responses to increasing temperature humidity index (THI) in lactating Murrah buffalo

The present study was conducted to determine the effect of increasing temperature humidity index (THI) on lactating Murrah buffalo. The study was conducted on six lactating buffaloes which were offered the same basal diet and were blocked by days in milk, milk yield and parity. The study was conducted during May, June and July with average THI of 79.88, 80.57 and 85.36, respectively. Milk yield was recorded twice a day at 06:00 and 18:00 h and physiological parameters were recorded and blood was sampled on 30th day of each month at 14:30 h. The results showed a significant decrease (P<0.05) in milk yield in buffaloes only at a THI of 85.36 as compared to THI up to 80.57. The rectal temperature (RT) did not change (P=0.580) with increasing THI up to 85.36, however, the respiration rate (RR) and pulse rate (PR) changed (P<0.05) progressively with increasing THI. Leucocytopnoea, lymphocytopnoea and neutrophilia; and alteration in serum urea, alkaline phosphatase activity, reactive oxygen species and phosphorus concentration were observed only at a THI of 85.36 as compared to THI up to 80.57. Similarly, significant (P<0.05) alteration in serum T4, cortisol and prolactin levels were observed at a THI of 85.36 as compared to THI up to 80.57. It can be concluded that lactating buffaloes experienced mild heat stress up to a THI of 80.57 (May and June) and a moderate type of heat stress at a THI 85.36 (July).

Dynamics of Fertility-Related Traits in Tomato Landraces under Mild and Severe Heat Stress.

Studies on the reproductive dynamics under heat stress are crucial to breed more tolerant cultivars. In tomato, cultivars, breeding lines, and wild species have been evaluated for their response to heat stress. Here, we addressed the study to a panel of selected landraces representing traditional genotypes that usually show high adaptation to local environments. In two experiments, spaced by 12 years, we set-up an identical experimental design with plants transplanted at two different dates to expose the second field to thermic stress with natural fluctuations. Such a strategy resulted in both a mild and severe stress in the two years. The landraces showed wide variation for both vegetative and reproductive traits; all traits were affected by heat, mostly with a significant Genotype*Environment interaction. A high broad-sense heritability was estimated for plant height, stigma position, pollen viability, and fruit weight. Low heritability estimates were found for the number of flowers, fruit set, and yield. Despite the interaction, traits recorded under control and heat conditions were positively correlated. Multivariate analysis located the genotypes in a topography that was stable under all conditions, except under the harshest temperatures. The study revealed that landraces present a wide variability for the response of reproductive traits to thermic challenges and that such a variation could be useful to dissect the traits with higher heritability and identify quantitative trait loci for breeding more resilient varieties.

Rectifying cow infertility under heat stress by immunization against inhibin and supplementation of progesterone

This study was undertaken for the development of novel techniques that are based on immunoneutralization of inhibin bioactivity to improve Holstein cow fertility. A series of 4 experiments were carried out on 2 farms that were located in subtropical or temperate regions, to test the effects of immunization against inhibin alpha subunit on cow fertility under varying degrees of heat stress conditions. Though immunization against inhibin alone improved conception rate (CR) after TAI moderately in cows under mild heat stress conditions, the treatment plus progesterone supplementation substantially enhanced CR in the range of 25 to 35 percentages from severe heat stress to comfortable weather conditions. There existed an additive effect between immunization against inhibin and progesterone supplementation that maximally enhanced CR. Further, immunization against inhibin increased both FSH and activin A concentrations in blood during both follicular and luteal phases. It also significantly increased blood concentrations of E2 in the follicular phase but decreased P4 concentrations during the early pregnancy. However, interferon-tau concentrations in blood around the time of pregnancy recognition were doubled in the inhibin immunized cows. In conclusion, immunization against inhibin plus P4 treatment enhances ovarian follicle and the subsequent early embryo developments that help to greatly improve the fertility of Holstein dairy cows.

Impact of heat stress of varying severity on papaya (Carica papaya) leaves: Major changes in stress volatile signatures, but surprisingly small enhancements of total emissions

Tropical plants are considered to be highly heat stress resistant, but heat waves of different frequency and duration induced by global climate change can exceed the plant heat stress tolerance in the tropics. To gain insight into the overall heat resistance, leaves of papaya were exposed to mild (37 and 41 °C), moderate (46 °C) and severe (49 °C) heat stress (applied for 5 min), and plant recovery was monitored for 48 h upon return to pre-stress conditions. Leaf photosynthetic characteristics were reduced immediately after stress in all cases, but photosynthesis rate almost fully recovered after mild heat treatment, and partly recovered in 48 h after moderate stress, while no recovery was observed under severe stress. Constitutive BVOC emissions dominated by long-chained aldehydes, and glucosinolate breakdown products, attained the emission maxima at 46 °C. Acute heat stress led only to a minor induction of BVOC emissions, and the greatest share of induced emissions was observed in leaves exposed to 46 °C. Induced emissions were dominated by oxygenated monoterpenes, indicating an increase in leaf oxidative status. Our results demonstrate that papaya could withstand moderately severe heat stress of up to 46 °C, but this level of stress will have negative impacts on plant carbon gain due to delayed photosynthetic recovery after heat stress. Although volatile profiles sensitively responded to heat stress, the magnitude and degree of elicitation were surprisingly small throughout the recovery, suggesting that papaya is relatively heat resistant and possess a suite of heat-protective traits. • Papaya photosynthetic recovery was rapid under mild heat stress, delayed under moderate stress and absent under severe stress. • Heat stress of different severity had only moderate effects on volatile emissions, but the composition of emitted volatiles was strongly altered. • Volatile emission blend rather than the absolute amount characterizes the impact of heat stress on leaves of papaya.

Response of Tomato-Pseudomonas Pathosystem to Mild Heat Stress

Higher plants suffer from mild heat stress when temperatures increase by 5 °C above optimum growth temperatures. This produces changes at the cellular and metabolic levels, allowing plants to adapt to heat conditions. This study investigated an increase of 5 °C above the optimum growth temperature (26 °C) of tomato plants in the tomato—Pseudomonas syringae pv. tomato pathosystem. A temperature increase above 26 °C affects plant development, the defensive pathways activated against Pseudomonas syringae pv. tomato strain DC3000 (PstDC3000), and the bacterial growth and virulence machinery. The results demonstrated that tomato plants were able to acclimate to mild heat stress, showing no symptoms of damage. Moreover, plants subjected to a 5 °C increase (T31 °C plants) showed higher basal levels of metabolites such as proline and putrescine, which probably act as compatible osmolytes. This demonstrates their importance as key components of thermotolerance. When grown under mild heat stress, plants were less susceptible to PstDC3000 and showed increased accumulation of abscisic acid, jasmonic acid-isoleucine, and spermine. In addition, the temperature increase negatively affected the infectivity of PstDC3000. Inhibition of the genes responsible for quorum sensing establishment and synthesis of flagellin and coronatine was observed in bacteria extracted from T31 °C plants. Analysis of the genes involved in the synthesis of the type III secretion system indicates the important role of this system in bacterial growth under these conditions. As the known resistance mechanisms involved in the defense against PstDC3000 were not activated, the changes in its virulence mechanisms under high temperatures may explain the lower infection observed in the T31 °C plants.

Tomato (Solanum lycopersicum L.) Genotypes Respond Differently to Long-Term Dry and Humid Heat Stress

Tomato production in coastal areas in West Africa is constrained by heat stress. There is currently limited empirical evidence on the extent of the effect of heat stress on tomato yield in the sub-region. In this study, we assessed the effects of heat stress on yield and yield components among 16 tomato genotypes with varying heat tolerance status and explored the potential of stress tolerance indices to identify heat tolerant genotypes. The experiments were conducted under three temperature and humidity regimes, namely optimal season (28.37/23.71 °C and 71.0/90.4% day/night), long-term mild and humid (greenhouse, 30.0/26.2 °C and 77.6/97.2%), and long-term mild and dry (open field, 31.50/28.88 °C and 66.72/77.82%) heat stress (HS). All genotypes exhibited significantly higher fruit set percentage, fruit number per plant, fruit weight, and fruit weight per plant in the optimal season compared to both heat stress conditions. In general, the genotypes demonstrated higher performance under dry HS (i.e., HS in open field HSO) than humid HS (i.e., HS in greenhouse HSG). Fruit set decreased by 71.5% and 68.3% under HSG and HSO, respectively, while a reduction of 75.1% and 50.5% occurred in fruit weight per plant under HSG and HSO, respectively. The average sum of ranks values from nine stress tolerance indices and fruit weight per plant (used as proxy trait of yield) identified CLN2498D, CLN3212C, CLN1621L, and BJ01 as heat tolerant under HSG and BJ01, BJ02, Fla.7171, and P005 as heat tolerant under HSO. Fruit weight per plant under long-term heat stress (Ys) and optimal growing conditions (Yp) were suitable to select high performing genotypes under HSO, HSG, and optimal conditions while relative stress index, yield stability index, yield index, stress susceptibility index, and harmonic mean were suitable to select heat tolerant genotypes under either HSG or HSO. Our findings shed light on the extent of the effect of HS on tomato production in the off-season in coastal areas in West Africa and provide new insight concerning the heat tolerance status of the evaluated tomato genotypes.

HsfA7 coordinates the transition from mild to strong heat stress response by controlling the activity of the master regulator HsfA1a in tomato.

Plants respond to higher temperatures by the action of heat stress (HS) transcription factors (Hsfs), which control the onset, early response, and long-term acclimation to HS. Members of the HsfA1 subfamily, such as tomato HsfA1a, are the central regulators of HS response, and their activity is fine-tuned by other Hsfs. We identify tomato HsfA7 as capacitor of HsfA1a during the early HS response. Upon a mild temperature increase, HsfA7 is induced in an HsfA1a-dependent manner. The subsequent interaction of the two Hsfs prevents the stabilization of HsfA1a resulting in a negative feedback mechanism. Under prolonged or severe HS, HsfA1a and HsfA7 complexes stimulate the induction of genes required for thermotolerance. Therefore, HsfA7 exhibits a co-repressor mode at mild HS by regulating HsfA1a abundance to moderate the upregulation of HS-responsive genes. HsfA7 undergoes a temperature-dependent transition toward a co-activator of HsfA1a to enhance the acquired thermotolerance capacity of tomato plants.

Fitness Analysis and Transcriptome Profiling Following Repeated Mild Heat Stress of Varying Frequency in Drosophilamelanogaster Females.

Simple SummaryWe studied the effect of mild heat stress (38 °C, 1 h) occurring once a day or once a week on D. melanogaster fertility, longevity, body composition metabolism and differential gene expression in fat body and adjacent tissues. Weekly stress in the first two weeks did not affect longevity but caused a decrease in fat content and an increase in the total level of fertility. Daily stress caused a significant longevity, fertility and fat content decrease, but an increase in carbohydrate levels compared with the control group. These data agree well with the results of transcriptome analysis, which demonstrated significant changes in expression levels of genes involved in proteolysis/digestion following daily stress. Heat shock protein 23 and stress-inducible humoral factor Turandot gene network are also involved. It is notable that daily and weekly heat stress resulted in different changes in metabolism, fitness and differential gene expression.Understanding how repeated stress affects metabolic and physiological functions in the long run is of crucial importance for evaluating anthropogenic pressure on the environment. We investigated fertility, longevity and metabolism in D. melanogaster females exposed to short-term heat stress (38 °C, 1 h) repeated daily or weekly. Daily stress was shown to cause a significant decrease in both fertility and longevity, as well as in body mass and triglyceride (fat) content, but a significant increase in trehalose and glucose content. Weekly stress did not affect longevity and carbohydrate metabolism but resulted in a significant decrease in body mass and fat content. Weekly stress did not affect the total level of fertility, despite sharp fertility drops on the exact days of stressing. However, stressing insects weekly, only in the first two weeks after eclosion, caused a significant increase in the total level of fertility. The analysis of differentially expressed genes in the fat bodies and adjacent tissues of researched groups with the use of RNA-Seq profiling revealed changes in signal pathways related to proteolysis/digestion, heat shock protein 23, and in the tightly linked stress-inducible humoral factor Turandot gene network.

PSI-18 Identifying genetic variants associated with grazing, walking, and slope use of cattle experiencing mild heat load

Abstract Rangeland is a valuable resource that can allow producers to cost effectively provide nutrients for grazing cattle. However, grazing behavior of cattle is adversely affected when the temperature humidity index is greater than 72. It is possible to select cattle that exhibit efficient grazing behavior even under mild heat stress. This study evaluated genetic associations with grazing behavior to help producers identify cows that will effectively use their rangeland pastures. Using genome-wide associations, this study identified single nucleotide polymorphisms (SNPs) associated with grazing time, walking time and max slope that cattle utilized while experiencing mild heat load. Data were collected from Angus X Hereford 2-year-old beef cows from UI herd over two years (37 grazing and walking minutes, 38 max slope). Genotypes were obtained using a Bovine GGP 50K SNP marker array and 41,686 markers were used in the analyses. Two SNPs on chromosome 11 are significantly (P = 5.01e-7, P = 6.46e-7) associated with grazing minutes and explain 0.52 proportion of variance (PVE). A SNP on chromosome 3 is significant for walking minutes (P=1.91e-6) with a PVE of 0.48. Additionally, a SNP on chromosome 14 is significantly (P = 8.50e-6) associated with max slope and has a PVE of 0.43. This ongoing project identified significant associations with grazing and walking minutes and maximum slope. This research will be strengthened with the addition of more animals over successive years. Some cattle spend more time grazing, walking, or at a higher elevation in mild heat load. Identifying genetic variants associated with grazing time, walking time, and maximum slope use while under heat stress can enable producers to select for cattle that best fit the rangeland available to them.

Renal Functional Reserve Is Attenuated During Mild Passive Heat Stress

PURPOSE: Whether glomerular filtration rate (GFR) is reduced with passive heat stress remains equivocal, with some studies showing no change in GFR and others showing a reduction. One explanation for these disparate findings is that GFR is only reduced when the magnitude of physiological challenge exceeds the capacity to recruit additional nephrons to sustain GFR. Thus, renal functional reserve, which is defined as the ability to increase GFR, typically in response to a protein load, is likely reduced with passive heat stress. However, this has never been reported. Therefore, we tested the hypothesis that renal functional reserve is reduced during passive heat stress. METHODS: Twelve healthy adults (26 ± 1 y, 3 females) completed two visits where they underwent passive heat stress or a normothermic time control trial in a randomized crossover design. In the heat stress trial, core temperature was increased ~0.8 °C over the first two hours and then subjects ingested a whey protein shake (1.2 g of protein/kg of body weight). Serum and urinary creatinine were measured pre-protein and 150 min post-protein ingestion. GFR was assessed via creatinine clearance (urinary creatinine multiplied by urine flow rate divided by serum creatinine). Data are presented as mean ± SD. RESULTS: The percent change in core temperature (0.8 ± 0.1 vs 0.3 ± 0.2 °C, P < 0.01) and body fluid loss (3.0 ± 0.9% vs. 1.5 ± 0.4%, P < 0.01) were greater in heat stress. Urinary creatinine was higher in heat stress pre- (96.2 ± 77.1 vs 36.9 ± 14.4 mg/dL, P = 0.05) and post- (191.7 ± 77.0 vs. 97.9 ± 47.7 mg/dL, P < 0.01) protein. Serum creatinine did not differ pre-protein between heat stress (1.00 ± 0.19 mg/dL) and normothermia (1.01 ± 0.17 mg/dL, P = 0.98), but was higher during heat stress post-protein (1.04 ± 0.22 vs. 0.96 ± 0.18 mg/dL, P = 0.02). Creatinine clearance did not differ between heat stress (135.1 ± 27.7 mg/dL) and normothermia (120.4 ± 33.1 mg/dL, P = 0.84) pre-protein and increased with protein in both trials (P = 0.04). However, increases in creatinine clearance with protein ingestion were attenuated with heat stress (17.1 ± 17.4 mg/dL) compared to normothermia (65.1 ± 66.7 mg/dL, P = 0.01). CONCLUSIONS: Mild passive heat stress reduces renal functional reserve, suggesting that heat stress reduces the ability to recruit nephrons to support GFR.Supported by R01OH011528.

Wnt signaling related transcripts and their relationship to energy metabolism in C2C12 myoblasts under temperature stress.

Temperature stress is one of the main environmental stressors affecting the welfare, health and productivity of livestock. Temperature changes can modify cell membrane components, disrupting the crosstalk between the cell and its surroundings by affecting signaling pathways including Wnt signaling pathway, which subsequently disrupts cell energy metabolism. The present study aims to understand the effect of temperature stress on the expression of genes involved in Wnt signaling pathways, and their interaction with energy metabolism in C2C12 myoblasts cells. The C2C12 cells were exposed to cold stress (35 °C), mild heat stress (39 °C) and severe heat stress (41 °C), whereas 37 °C was used as control temperature. Transcript levels of important genes involved in Wnt signaling including Axin2, Tnks2, Sfrp1, Dkk1, Dact1, Cby1, Wnt5a, Wnt7a, Wnt11, Porcn, Ror2, Daam1, and Ppp3ca were significantly altered under severe heat stress (41 °C), whereas eight Wnt signaling-related transcripts (Daam1, Ppp3ca, Fzd7, Wnt5a, Porcn, Tnks2, Lrp6, and Aes) were significantly altered under cold stress (35 °C) compared to control. Under heat stress transcripts of the Wnt/β-catenin inhibitors (Sfrp1, Dkk1, and Cby1) and negative regulators (Dact1 and Axin2) are activated. A positive correlation between oxidative phosphorylation and Wnt-related transcripts was found under high temperatures. Transcripts of the cell membrane receptors, including Lrp6 and Fzd7, and the members of Wnt/Ca+2 signaling pathway, including Ppp3ca and Porcn were downregulated under cold stress. Many Wnt signaling-related transcripts were positively correlated with glycolysis under cold stress. These findings indicate a cross-talk between Wnt signaling and energy metabolism under thermal stress.

Jejunal mucosa proteomics unravel metabolic adaptive processes to mild chronic heat stress in dairy cows

Climate change affects the duration and intensity of heat waves during summer months and jeopardizes animal health and welfare. High ambient temperatures cause heat stress in dairy cows resulting in a reduction of milk yield, feed intake, and alterations in gut barrier function. The objectives of this study were to investigate the mucosal amino acid, glucose and lactate metabolism, as well as the proteomic response of the small intestine in heat stressed (HS) Holstein dairy cows. Cows of the HS group (n = 5) were exposed for 4 days to 28 °C (THI = 76) in a climate chamber. Percentage decrease in daily ad libitum intake of HS cows was calculated to provide isocaloric energy intake to pair-fed control cows kept at 15 °C (THI = 60) for 4 days. The metabolite, mRNA and proteomic analyses revealed that HS induced incorrect protein folding, cellular destabilization, increased proteolytic degradation and protein kinase inhibitor activity, reduced glycolysis, and activation of NF-κB signaling, uronate cycling, pentose phosphate pathway, fatty acid and amino acid catabolism, mitochondrial respiration, ATPase activity and the antioxidative defence system. Our results highlight adaptive metabolic and immune mechanisms attempting to maintain the biological function in the small intestine of heat-stressed dairy cows.

Distinct spatiotemporal distribution of Hsp90 under high-heat and mild-heat stress conditions in fission yeast.

The molecular chaperone Hsp90 is highly conserved from bacteria to mammals. In fission yeast, Hsp90 is essential in many cellular processes and its expression is known to be increased by heat stress (HS). Here, we describe the distinct spatiotemporal distribution of Hsp90 under high-heat stress (HHS: 45˚C) and mild-heat stress (MHS: 37˚C). Hsp90 is largely distributed in the cytoplasm under non-stressed conditions (27˚C). Under HHS, Hsp90 forms several cytoplasmic granules within 5 minutes, then the granules disappear within 60 minutes. Under MHS, Hsp90 forms fewer granules than under HHS within 5 minutes and strikingly the granules persist and grow in size. In addition, nuclear enrichment of Hsp90 was observed after 60 minutes under both HS conditions. Our data suggest that assembly/disassembly of Hsp90 granules is differentially regulated by temperatures.

Renal Vasoconstrictor, but Not Vasodilator, Responses are Modified by Mild Passive Heat Stress in Humans

Purpose The effect of heat stress on renal vascular control is not well studied. For instance, moderate passive heat stress attenuates the renal segmental artery vasoconstrictor response to the cold pressor test (CPT), a sympathoexcitatory stimulus. Whether this occurs with mild passive heat stress is unknown. Additionally, the vasodilatory response of the kidneys has never been studied during heat stress. Oral protein loading is a common approach used to elicit increases in renal blood flow. The purpose of this study was to test the hypothesis that both renal segmental artery vasoconstriction and vasodilation are attenuated during mild passive heat stress. Methods Twelve healthy adults (26 ± 1 y, 3 females) completed two experimental visits where they underwent passive heat stress or a normothermic time control trial in a randomized crossover design. In the heat stress trial, core temperature was increased ~0.5°C within the first hour and was maintained thereafter. Subjects completed a CPT one hour into heat stress which involved subjects immersing their right hand in an agitated ice slurry for 2 min. One hour post-CPT, subjects ingested a whey protein shake (1.2 g of protein/kg of body weight mixed with water). Renal segmental artery blood velocity (BV, Doppler ultrasound) and beat-to-beat blood pressure (Penaz method) were measured pre-CPT, in the final minute of CPT, pre-protein, and 150 min post-protein. Segmental artery vascular resistance (VR) was calculated as mean arterial pressure (MAP) divided by segmental artery BV. Data are presented as mean ± SD. Results Core temperature was higher pre-CPT (37.6 ± 0.5 vs 37.1 ± 0.5°C, P<0.01) and pre-protein (37.7 ± 0.4 vs 37.0 ± 0.4°C, P<0.01), and body fluid loss was greater (3.0 ± 0.9% vs. 1.5 ± 0.4%, P<0.01) in heat stress compared to normothermia. MAP did not differ pre-CPT between heat stress (80 ± 11 mmHg) and normothermia (84 ± 9 mmHg, P=0.76), but during the CPT was lower during heat stress (88 ± 18 vs. 113 ± 14 mmHg, P<0.01). Segmental BV did not differ pre-CPT between heat stress (22 ± 3 cm/s) and normothermia (22 ± 4 cm/s, P=0.99) or during the CPT (P = 0.56). Segmental VR pre-CPT did not differ between heat stress (3.8 ± 0.6 mmHg/cm/s) and normothermia (4.0 ± 0.7 mmHg/cm/s, P=0.91) but was lower in heat stress during the CPT (5.7 ± 1.1 vs. 4.6 ± 1.5 mmHg/cm/s, P=0.01). MAP did not differ pre-protein between heat stress (83 ± 9 mmHg) and normothermia (85 ± 9 mmHg, P=0.93), or post-protein (P=0.94). Segmental BV did not differ pre-protein between heat stress (22 ± 3 cm/s) and normothermia (22 ± 3 cm/s, P=0.99), or post-protein (P=0.36). Segmental VR was not different pre-protein between heat stress (3.9 ± 0.8 mmHg/cm/s, P=0.97) and normothermia (3.9 ± 0.6 mmHg/cm/s). Segmental VR decreased post-protein (P=0.03) but did not differ between heat stress (3.4 ± 0.9 mmHg/cm/s) and normothermia (3.5 ± 0.5 mmHg/cm/s, P=0.98). Conclusion Compared to normothermia, renal segmental artery vasoconstriction during a sympathetic stimulus is attenuated, but renal segmental artery vasodilation following a protein load is not affected by mild passive heat stress.

The Putative RNA-Binding Protein Dri1 Promotes the Loading of Kinesin-14/Klp2 to the Mitotic Spindle and Is Sequestered into Heat-Induced Protein Aggregates in Fission Yeast.

Cells form a bipolar spindle during mitosis to ensure accurate chromosome segregation. Proper spindle architecture is established by a set of kinesin motors and microtubule-associated proteins. In most eukaryotes, kinesin-5 motors are essential for this process, and genetic or chemical inhibition of their activity leads to the emergence of monopolar spindles and cell death. However, these deficiencies can be rescued by simultaneous inactivation of kinesin-14 motors, as they counteract kinesin-5. We conducted detailed genetic analyses in fission yeast to understand the mechanisms driving spindle assembly in the absence of kinesin-5. Here, we show that deletion of the dri1 gene, which encodes a putative RNA-binding protein, can rescue temperature sensitivity caused by cut7-22, a fission yeast kinesin-5 mutant. Interestingly, kinesin-14/Klp2 levels on the spindles in the cut7 mutants were significantly reduced by the dri1 deletion, although the total levels of Klp2 and the stability of spindle microtubules remained unaffected. Moreover, RNA-binding motifs of Dri1 are essential for its cytoplasmic localization and function. We have also found that a portion of Dri1 is spatially and functionally sequestered by chaperone-based protein aggregates upon mild heat stress and limits cell division at high temperatures. We propose that Dri1 might be involved in post-transcriptional regulation through its RNA-binding ability to promote the loading of Klp2 on the spindle microtubules.

Dynamics of heat shock protein responses to thermal stress changes after metamorphosis in a lepidopteran insect.

In the last decade, unexpected high temperatures have been frequent in spring and early summer. Numerous studies have shown that such thermal stress has substantial effects on life-history traits that influence fitness of insects, but few have examined expression dynamics of heat shock proteins (Hsps) across developmental stages, especially as regards potential carry-over effects at the transcriptional level across metamorphosis. We exposed pupae of the oriental fruit moth ("OFM,"Grapholita molesta Busck) to mild heat stress (38°C, 6 h) and then quantified expression patterns of six Hsps (Hsp90, 70, 60, 40, 21, and 11) from pupal through adult stages. Almost all Hsps showed a higher expression immediately after pupae were heat-stressed, but later dropped to normal levels after metamorphosis. Although upregulation of Hsps is transient and the effects carry over longer to early adult stage, upregulation will nonetheless have positive effects on adult fitness. The fitness of some insects may benefit from higher expression of chaperon genes after mild stress, in the form of higher fecundity and longer lifespan, as a carry-over effect. These results suggest that mild thermal stress can change genetic expression that later boosts adult fitness through a cascade effect.

Nutritional Intervention Strategies Using Dietary Antioxidants and Organic Trace Minerals to Reduce the Incidence of Wooden Breast and Other Carcass Quality Defects in Broiler Birds.

Wooden breast (WB) is a degenerative myopathy seen in modern broiler birds resulting in quality downgrade of breast fillets. Affected filets show increased toughness both before as well as after cooking and have decreased water holding capacity and marinade pick up compared to normal fillets. Although the exact etiology is unknown, the circulatory insufficiency and increased oxidative stress in the breast muscles of modern broiler birds could be resulting in damage and degeneration of muscle fibers leading to myopathies. Three independent experiments were conducted to evaluate the effect of various dietary interventions on the incidence of WB when birds are exposed to oxidative stress associated with feeding oxidized fat and mild heat stress. Feed additives such as dietary antioxidant [Ethoxyquin (ETX)], mineral methionine hydroxy analog chelate (MMHAC) of Zn, Cu, and Mn, and organic selenium (Org Se) were tested at recommended levels. In experiment 1, ETX reduced (P < 0.05) the incidence of severe WB induced by oxidized fat diet. The magnitude of improvement in percentage of normal (no WB) filets and reduction in muscle lipid peroxidation was greater (P < 0.05) when ETX and MMHAC were fed together as shown by experiment 2. In birds exposed to mild heat stress (Experiment 3), feeding MMHAC by itself reduced (P < 0.05) tissue damage by reducing incidence of tibial head lesions, skin scratches, breast blisters, in addition to increasing the incidence of normal (no WB) fillets. When MMHAC was combined with ETX and Org Se, further improvement (P < 0.05) in normal (no WB) filets was observed. In summary, under different oxidative stress conditions, dietary intervention programs that contain ETX, MMHA-Zn, -Cu, and -Mn and Org Se can improve performance and increase carcass integrity, reducing problems, such as WB, either independently or with additive effect. This effect is most likely attained by simultaneously improving the exogenous and endogenous antioxidant status, reducing oxidative stress, and improving tissue healing process of the bird.

An advanced empirical model for quantifying the impact of heat and climate change on human physical work capacity

Occupational heat stress directly hampers physical work capacity (PWC), with large economic consequences for industries and regions vulnerable to global warming. Accurately quantifying PWC is essential for forecasting impacts of different climate change scenarios, but the current state of knowledge is limited, leading to potential underestimations in mild heat, and overestimations in extreme heat. We therefore developed advanced empirical equations for PWC based on 338 work sessions in climatic chambers (low air movement, no solar radiation) spanning mild to extreme heat stress. Equations for PWC are available based on air temperature and humidity, for a suite of heat stress assessment metrics, and mean skin temperature. Our models are highly sensitive to mild heat and to our knowledge are the first to include empirical data across the full range of warm and hot environments possible with future climate change across the world. Using wet bulb globe temperature (WBGT) as an example, we noted 10% reductions in PWC at mild heat stress (WBGT = 18°C) and reductions of 78% in the most extreme conditions (WBGT = 40°C). Of the different heat stress indices available, the heat index was the best predictor of group level PWC (R2 = 0.96) but can only be applied in shaded conditions. The skin temperature, but not internal/core temperature, was a strong predictor of PWC (R2 = 0.88), thermal sensation (R2 = 0.84), and thermal comfort (R2 = 0.73). The models presented apply to occupational workloads and can be used in climate projection models to predict economic and social consequences of climate change.