Introduction: pressure injuries (PI) in critically ill neonates are a health problem with a negative impact on morbidity, mortality, and healthcare costs. Knowledge and management of PI prevention by healthcare professionals are crucial for improving clinical practice. Objective: to analyze the level of knowledge, perceptions, and practices of midwives in Neonatal Critical Care Units (NCCU) regarding PI prevention. Material and Methods: a quantitative, descriptive, non-experimental, cross-sectional study with a sample of 32 midwives from three NCCUs in the Fifth Region of Chile. The validated PREVINTLPP questionnaire was used. Results: knowledge of PI prevention was insufficient (average 64,8 % minimum 70 % to be considered adequate), with great variability in responses. Adherence to recommended clinical practice was even lower (43,5 % on average). There is awareness that PIs are a quality indicator, but 96,9 % are unaware of international guidelines for their management. Conclusions: Knowledge of PI prevention in the study sample is inadequate, possibly due to poor knowledge management. Further investigation into the causes and implementation of specialized training programs are needed to improve the quality of care and patient safety.
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