In this study we recorded auditory brainstem responses to airborne sounds to determine the hearing sensitivity of Xenopus laevis frogs and correlated their hearing profiles with middle ear characteristics. In newly metamorphosed frogs (body mass 0.5-0.76 gm, snout-vent length 17-20 mm) best hearing sensitivities were measured in the 2.4-2.8 kHz range, whereas optimal hearing sensitivity of older adults (body mass 18-90 gm; snout-vent length 57-100 mm) ranged from 1.0 to 1.2 kHz. Middle ear volumes reconstructed from serial sections showed approximate volume of 0.002 cc and 0.04-0.07 cc in newly metamorphosed and older frogs, respectively. This inverse frequency-volume relationship is consistent with the properties of an acoustic resonator indicating that differences in best hearing sensitivity are at least in part correlated to variation in middle ear volumes for airborne sounds. These results are consistent with peak frequency vibrational velocity profiles of Xenopus tympanic disk that have been shown to be dependent on underlying middle ear volumes and corroborate the occurrence of peak amplitudes of otoacoustic emissions in the 1.0-1.2 kHz region in adult Xenopus frogs.