Mid-frequency Region Research Articles

No Evidence from FTIR Difference Spectroscopy That Glutamate-189 of the D1 Polypeptide Ligates a Mn Ion That Undergoes Oxidation during the S0 to S1, S1 to S2, or S2 to S3 Transitions in Photosystem II

In the recent X-ray crystallographic structural models of photosystem II, Glu189 of the D1 polypeptide is assigned as a ligand of the oxygen-evolving Mn(4) cluster. To determine if D1-Glu189 ligates a Mn ion that undergoes oxidation during one or more of the S(0) --> S(1), S(1) --> S(2), and S(2) --> S(3) transitions, the FTIR difference spectra of the individual S-state transitions in D1-E189Q and D1-E189R mutant PSII particles from the cyanobacterium Synechocystis sp. PCC 6803 were compared with those in wild-type PSII particles. Remarkably, the data show that neither mutation significantly alters the mid-frequency regions (1800-1200 cm(-)(1)) of any of the FTIR difference spectra. Importantly, neither mutation eliminates any specific symmetric or asymmetric carboxylate stretching mode that might have been assigned to D1-Glu189. The small spectral alterations that are observed are similar in amplitude to those that are observed in wild-type PSII particles that have been exchanged into FTIR analysis buffer by different methods or those that are observed in D2-H189Q mutant PSII particles (the residue D2-His189 is located >25 A from the Mn(4) cluster and accepts a hydrogen bond from Tyr Y(D)). The absence of significant mutation-induced spectral alterations in the D1-Glu189 mutants shows that the oxidation of the Mn(4) cluster does not alter the frequencies of the carboxylate stretching modes of D1-Glu189 during the S(0) --> S(1), S(1) --> S(2), or S(2) --> S(3) transitions. One explanation of these data is that D1-Glu189 ligates a Mn ion that does not increase its charge or oxidation state during any of these S-state transitions. However, because the same conclusion was reached previously for D1-Asp170, and because the recent X-ray crystallographic structural models assign D1-Asp170 and D1-Glu189 as ligating different Mn ions, this explanation requires that (1) the extra positive charge that develops on the Mn(4) cluster during the S(1) --> S(2) transition be localized on the Mn ion that is ligated by the alpha-COO(-) group of D1-Ala344 and (2) any increase in positive charge that develops on the Mn(4) cluster during the S(0) --> S(1) and S(2) --> S(3) transitions be localized on the one Mn ion that is not ligated by D1-Asp170, D1-Glu189, or D1-Ala344. An alternative explanation of the FTIR data is that D1-Glu189 does not ligate the Mn(4) cluster. This conclusion would be consistent with earlier spectroscopic analyses of D1-Glu189 mutants, but would require that the proximity of D1-Glu189 to manganese in the X-ray crystallographic structural models be an artifact of the radiation-induced reduction of the Mn(4) cluster that occurred during the collection of the X-ray diffraction data.

A method for structural vibration characterization in the midfrequency region

The ability to characterize elastic structures at higher frequencies in a meaningful and convenient manner has been a topic of much interest. Many analytical or experimental approaches incorporate the use of lumped quantities to represent critical system characteristics at discrete locations. When the excitation frequency increases, the structural wavelength becomes comparable to the dimensions of that contact region and the point-quantity assumption is no longer valid. To provide a similar means for describing structures at higher frequencies, the work described here reformulates the traditional four-pole method in terms of quantities defined over planes. Spatial variations of the important response characteristics across the connecting regions can be considered. As for the four-pole method, the approach has the advantage of providing building blocks for simple structures in order to represent more complex structures. Three application examples are presented. One example involves modeling a simple vibration isolator. In another example, the impact of having spatial functions that extend over only a portion of the structure is studied. Finally, a two-dimensional structure is examined using a finite element approach. In using the approach, additional insight can be gained that cannot be easily found using typical finite element method analysis results.

Determining the MTF of Medical Imaging Displays Using Edge Techniques

The modulation transfer function (MTF) of a medical imaging display is typically determined by measuring its response to square waves (bar patterns), white noise, and/or line stimuli. However, square waves and white noise methods involve capture and analysis of multiple images and are thus quite tedious. Measurement of the line-spread function (LSF) offers a good alternative. However, as previously reported, low-frequency response obtained from the LSF method is not as good as that obtained from measurement of edge-spread function (ESF). In this paper, we present two methods for evaluating the MTF of a medical imaging display from its ESF. High degree of accuracy in the higher frequency region (near the Nyquist frequency of the system) was achieved by reducing the noise. In the first method, which is a variant of the Gans' original method, the periodic raster noise is reduced by subtracting a shifted ESF from the ESF. The second method employs a low-pass differentiator (LPD). A novel near maximally flat LPD with the desired cut-off frequency was designed for this purpose. Noise reduction in both the methods was also achieved by averaging over large portions of the image data to form the ESF. Experimental results show that the MTF obtained by these methods is comparable to that obtained from the square wave response. Furthermore, the MTFs of rising and falling edges of a cathode ray tube (CRT) were measured. The results show that the rising and falling vertical MTFs are practically the same, whereas the rising horizontal MTF is poorer than the falling horizontal MTF in the midfrequency region.

No Evidence from FTIR Difference Spectroscopy That Aspartate-170 of the D1 Polypeptide Ligates a Manganese Ion That Undergoes Oxidation during the S0 to S1, S1 to S2, or S2 to S3 Transitions in Photosystem II

On the basis of mutagenesis and X-ray crystallographic studies, Asp170 of the D1 polypeptide is widely believed to ligate the (Mn)4 cluster that is located at the catalytic site of water oxidation in photosystem II. Recent proposals for the mechanism of water oxidation postulate that D1-Asp170 ligates a Mn ion that undergoes oxidation during one or more of the S0 --> S1, S1 --> S2, and S2 --> S3 transitions. To test these hypotheses, we have compared the FTIR difference spectra of the individual S state transitions in wild-type* PSII particles from the cyanobacterium Synechocystis sp. PCC 6803 with those in D1-D170H mutant PSII particles. Remarkably, our data show that the D1-D170H mutation does not significantly alter the mid-frequency regions (1800-1000 cm(-1)) of any of the FTIR difference spectra. Therefore, we conclude that the oxidation of the (Mn)4 cluster does not alter the frequencies of the carboxylate stretching modes of D1-Asp170 during the S0 --> S1, S1 --> S2, or S2 --> S3 transitions. The simplest explanation for these data is that the Mn ion that is ligated by D1-Asp170 does not increase its charge or oxidation state during any of these S state transitions. These data have profound implications for the mechanism of water oxidation. Either (1) the oxidation of the Mn ion that is ligated by D1-Asp170 occurs only during the transitory S3 --> S4 transition and serves as the critical step in the ultimate formation of the O-O bond or (2) the oxidation increments and O2 formation chemistry that occur during the catalytic cycle involve only the remaining Mn3Ca portion of the Mn4Ca cluster. Our data also show that, if the increased positive charge on the (Mn)4 cluster that is produced during the S1 --> S2 transition is delocalized over the (Mn)4 cluster, it is not delocalized onto the Mn ion that is ligated by D1-Asp170.

Structural Changes of D1 C-terminal α-Carboxylate during S-state Cycling in Photosynthetic Oxygen Evolution

Changes in the chemical structure of alpha-carboxylate of the D1 C-terminal Ala-344 during S-state cycling of photosynthetic oxygen-evolving complex were selectively measured using light-induced Fourier transform infrared (FTIR) difference spectroscopy in combination with specific [(13)C]alanine labeling and site-directed mutagenesis in photosystem II core particles from Synechocystis sp. PCC 6803. Several bands for carboxylate symmetric stretching modes in an S(2)/S(1) FTIR difference spectrum were affected by selective (13)C labeling of the alpha-carboxylate of Ala with l-[1-(13)C]alanine, whereas most of the isotopic effects failed to be induced in a site-directed mutant in which Ala-344 was replaced with Gly. Labeling of the alpha-methyl of Ala with l-[3-(13)C]alanine had much smaller effects on the spectrum to induce isotopic bands due to a symmetric CH(3) deformation coupled with the alpha-carboxylate. The isotopic bands for the alpha-carboxylate of Ala-344 showed characteristic changes during S-state cycling. The bands appeared prominently upon the S(1)-to-S(2) transition and to a lesser extent upon the S(2)-to-S(3) transition but reappeared at slightly upshifted frequencies with the opposite sign upon the S(3)-to-S(0) transition. No obvious isotopic band appeared upon the S(0)-to-S(1) transition. These results indicate that the alpha-carboxylate of C-terminal Ala-344 is structurally associated with a manganese ion that becomes oxidized upon the S(1)-to-S(2) transition and reduced reversely upon the S(3)-to-S(0) transition but is not associated with manganese ion(s) oxidized during the S(0)-to-S(1) (and S(2)-to-S(3)) transition(s). Consistently, l-[1-(13)C]alanine labeling also induced spectral changes in the low frequency (670-350 cm(-1)) S(2)/S(1) FTIR difference spectrum.

Smart Panel Technology for Broadband Noise Reduction

The possibility of a broadband noise reduction by piezoelectric smart panels is demonstrated. A smart panel is basically a plate structure on which piezoelectric patches with electrical shunt circuits are mounted and sound absorbing material is bonded on the surface of the structure. Sound absorbing material can absorb the sound transmitted at the mid frequency region effectively while the use of piezoelectric shunt damping can reduce the transmission at resonance frequencies of the panel structure. To be able to reduce the sound transmission at low panel resonance frequencies, piezoelectric damping is adopted. A resonant shunt circuit for piezoelectric shunt damping is composed of resistor and inductor in series, and they are determined by maximizing the dissipated energy through the circuit. The transmitted noise reduction performance of smart panels was tested in an acoustic tunnel. Sound absorbing material and air gap reduces the sound transmitted at the mid-frequency region effectively while the use of piezoelectric shunt damping works at the low frequency region. As a result, a remarkable noise reduction of 9-15dB was achieved in broadband frequency. Double panel exhibits better noise reduction than other panels. This approach was also applied to the structural acoustic problem of turbulent boundary layer (TBL) induced sound radiated from a panel, and the possibility of TBL noise reduction was demonstrated. The hybrid approach of smart panels is a promising technology for noise reduction over broad frequency band.

A basic hybrid finite element formulation for mid-frequency analysis of beams connected at an arbitrary angle

When beams are connected at an arbitrary angle and subjected to an external excitation, both longitudinal and bending waves are generated in the system. Since longitudinal wavelengths are considerably longer than bending wavelengths in the mid-frequency region, the number of bending wavelengths in the beams is considerably larger than the number of longitudinal wavelengths. In this paper, plannar beams connected at arbitrary angles are considered. The energy finite element analysis (EFEA) is employed for modelling the bending behavior of the beams and the conventional finite element analysis (FEA) is utilized for modelling the longitudinal vibration in the beams. Thus, a basic hybrid FEA formulation is presented for mid-frequency analysis of systems that contain two types of energy. The bending vibration is associated with the long members in the system and the longitudinal vibration is associated with the short members. The long members are considered to have high modal overlap and to contain several wavelengths within their dimension, and uncertainty effects are present. The short members contain a small number of wavelengths, and exhibit a low modal overlap. Due to the low modal overlap the resonant frequencies are spaced far apart in the frequency domain, therefore the short members exhibit resonant or non-resonant behavior depending on the frequency of the excitation. In this work, the bending and the longitudinal vibration within the same beam member are treated as a long and as a short member, respectively. A hybrid joint formulation is developed between long and short members. Power reflection and transmission coefficients are derived for each joint. The distribution of the energy throughout the system demonstrates a strong dependency on the power transfer coefficients. Several systems are analyzed by the hybrid FEA and by analytical solutions, and good correlation between them is observed.

Substructure analysis of complex systems using rigid body information of components

Frequency response function (FRF) based substructure analysis can predict the response of complex systems using the FRFs of substructures. It combines the FRFs of each substructure derived from finite element analysis or experiments depending on the situation. In general, the substructure with the excitation is separated from the others by rubber bushes to prevent the transmission of vibration from the source to the main structure. In this case, the substructure with the excitation shows rigid body motion up to the mid-frequency region. This paper presents a new FRF-based substructure analysis that uses the FRFs from the rigid body information not from the complex finite element model of the substructure with rigid body motion. The rigid body information including the mass, the moment of inertia and the coordinates of the mass centre comes from the computer-aided design data. Since the mechanism of this technique is very similar to the finite element formation, it can be applied to complex systems with ease. Through a simple example of a ladder structure and a practical example of the interior noise in a car, the accuracy and efficiency of this approach is proven.

Broadband transmission noise reduction of smart panels featuring piezoelectric shunt circuits and sound-absorbing material.

The possibility of a broadband noise reduction of piezoelectric smart panels is experimentally studied. A piezoelectric smart panel is basically a plate structure on which piezoelectric patches with electrical shunt circuits are mounted and sound-absorbing material is bonded on the surface of the structure. Sound-absorbing material can absorb the sound transmitted at the midfrequency region effectively while the use of piezoelectric shunt damping can reduce the transmission at resonance frequencies of the panel structure. To be able to reduce the sound transmission at low panel resonance frequencies, piezoelectric damping using the measured electrical impedance model is adopted. A resonant shunt circuit for piezoelectric shunt damping is composed of resistor and inductor in series, and they are determined by maximizing the dissipated energy through the circuit. The transmitted noise-reduction performance of smart panels is tested in an acoustic tunnel. The tunnel is a square cross-sectional tube and a loudspeaker is mounted at one side of the tube as a sound source. Panels are mounted in the middle of the tunnel and the transmitted sound pressure across panels is measured. When an absorbing material is bonded on a single plate, a remarkable transmitted noise reduction in the midfrequency region is observed except for the fundamental resonance frequency of the plate. By enabling the piezoelectric shunt damping, noise reduction is achieved at the resonance frequency as well. Piezoelectric smart panels incorporating passive absorbing material and piezoelectric shunt damping is a promising technology for noise reduction over a broadband of frequencies.

Frequency specificity of cochlear damage in acute electrical injury: a longitudinal distortion product otoacoustic emission study.

To understand cochlear dysfunction and the recovery pattern of the cochlea after acute electrical injury. The cochlea is believed to be more vulnerable to direct current than to alternating current. However, the damage-and frequency-specific recovery characteristics of the cochlea have not been well described. Baseline distortion product otoacoustic emission measurements were taken via transtympanic electrodes from 19 guinea pigs. A 20-Hz alternating current and a positive direct current, both at a 1,000-microA intensity, were applied to 9 and 10 animals, respectively. The measurements were repeated immediately after the application of current and after 10 days. Comparisons were made for both groups individually in signal-to-noise ratios obtained before and immediately after, immediately and 10 days after, and before and 10 days after the application of electrical current. Alternating and direct currents caused a significant depression in signal-to-noise ratio immediately after the application. However, 10 days later, the mean signal-to-noise ratio in the animals subjected to alternating current came significantly close to the baseline value, particularly between the frequencies of 2,211 and 3,717 Hz. By contrast, the mean signal-to-noise ratio in the animals subjected to direct current remained significantly depressed throughout all frequencies except for 2,211 Hz. Baseline distortion product otoacoustic emission measurements enabled cochlear function to be monitored in a frequency-specific manner after electrical injury. The functional damages were quantitatively close to each other for both types of currents at a given intensity. Nevertheless, recovery was more apparent in the animals exposed to alternating current than in those exposed to direct current. Recovery was also better in the midfrequency region than in higher frequencies. It is hypothesized that the reason for the difference in recovery in both groups was the net charge left by direct current.

Fingerprinting petroporphyrin structures with vibrational spectroscopy. Part 6: resonance Raman characterization of regioisomers of nickel(II) benzoetioporphyrin

Nickel(II) complexes of the geochemically significant four regioisomers of benzoetioporphyrin, Ni(BP–A–D), which contain a benzene ring fused onto the Cβ atoms of a pyrrole ring, have been synthesized and structurally characterized by resonance Raman spectroscopy. Laser excitations in resonance with the porphyrin Soret (406.7 nm) and Q (530.9 and 568.2 nm) electronic absorption bands exposed nearly all Raman active vibrations in the fingerprint region (100–1700 cm−1). The porphyrin skeletal vibrations above 1300 cm–1 are largely unaffected by the different location of the β,β-benzo exocyclic ring, but their frequencies indicate slightly more planar structures in solution for Ni(BP) porphyrins relative to nickel tetrahydrobenzo- and etioporphyrins. Several unique marker bands are also found for vibrations of the β,β-benzo substituent, especially in the Soret-band resonant spectra. Alkyl substituent and porphyrin skeletal vibrations in the low- (350–550 cm−1) and mid-frequency (750–1300 cm−1) regions show striking sensitivity to small conformational changes in the porphyrin, allowing the four Ni(BP) regioisomers to be readily distinguished.

Experimental validation of RESOUND mid-frequency acoustic radiation models

RESOUND is a full-spectrum structural acoustic analysis method that uses finite element analysis in the low frequency region, statistical energy analysis in the high frequency region and a hybrid approach in the mid frequency region. In this paper, acoustic radiation from a frame-stiffened panel into a large acoustic space will be investigated. Over the frequency range of interest, the frame has relatively few modes and exhibits long wavelength global behavior, while the panel has a large number of modes and exhibits short wavelength local behavior. Numerical and experimental results will be presented which illustrate how the frame and panel interact to give rise to the radiated sound field.

Backscattering of transients by tilted truncated cylindrical shells: time-frequency identification of ray contributions from measurements.

Impulse backscattering measurements by a thick-walled finite cylindrical shell are examined in the time-frequency domain to identify and characterize individual ray contributions from generalized Lamb waves excited on the shell. Previous experiments and analysis in the frequency-aspect angle domain [S. F. Morse et al., J. Acoust. Soc. Am. 103, 785-794 (1998)] indicate that large backscattering enhancements occur in the midfrequency region for the shell tilted at large angles. Presently this experimental data is examined in the time-frequency domain for selected angles of incidence. Individual ray contributions are evident and their evolution over aspect angle is discussed. The most prominent contribution is due to the meridional ray of the a0 leaky Lamb wave. This feature distinctly highlights the truncation of the shell and is found over a range of aspect angles spanning 200 degrees for the frequencies examined. Also observed are periodic features corresponding to end-reflected helical waves of the a0-. These scattering features are significantly different from those reported for thin-walled finite cylinders at low frequencies. The present results may be useful for target identification and localization and as a comparison tool for high-frequency computational scattering models.

Influence of Contralateral Stimulation by Two-tone Complexes, Narrow-band and Broad-band Noise Signals on the 2f 1 -f 2 Distortion Product Otoacoustic Emission Levels in Humans

In order to test the frequency specificity of the efferent suppressive effect on otoacoustic emissions, changes in the 2f 1 -f 2 distortion product otoacoustic emission (DPOAE) levels induced by contralateral stimuli of different spectra were measured in 10 normally hearing adults. Three types of contralateral stimuli were used: ( i ) a set of 6 pairs of pure tones with the same frequencies as used for DPOAE stimulation; ( ii ) 6 narrow-band noise signals with cut-off frequencies equal to the frequencies of the primary tones used for DPOAE stimulation; and ( iii ) broad-band noise with a bandwidth of 840-6,000 Hz. A small suppressive effect was observed mainly in the mid-frequency region. Broad-band noise was more effective at suppressing DPOAEs than narrow-band noises and two-tone complexes. Occasionally, small enhancements in DPOAE amplitudes were observed. Based on the results of this study, it is concluded that DPOAE changes induced by contralateral stimuli are not frequency-specific, and are too small to have routine clinical value.

The use of psychophysical tuning curves to explore dead regions in the cochlea.

"Dead regions" are regions in the cochlea with no functioning inner hair cells (IHCs) and/or neurons. Amplification (using a hearing aid) over a frequency range corresponding to a dead region may not be beneficial and may even impair speech intelligibility. The objective of this article is to illustrate the use of psychophysical tuning curves (PTCs) as a tool for investigating dead regions and to illustrate the variety of audiogram configurations that can be associated with dead regions. We explore the influence of signal level and signal frequency to test the hypothesis that the frequency at the tip of the tuning curve defines the boundary of the dead region. PTCs were measured for five subjects with sensorineural hearing loss who were suspected of having dead regions. One had a relatively "flat" loss, one had a mild mid-frequency loss and three had high-frequency losses, varying in severity from 70 dB to more than 120 dB. For each PTC, the level and frequency of the sinusoidal signal were fixed, and the level of a narrowband noise masker needed just to mask the signal was determined as a function of the masker frequency. When the signal falls in a frequency region that is not "dead," the signal is detected via IHCs with characteristic frequencies (CFs) at or close to the signal frequency. In such a case, the tip of the PTC (the masker frequency at which the masker level is lowest) lies at or close to the signal frequency. When a dead region is present, the signal is detected via IHCs with CFs different from that of the signal frequency. In such a case, the tip of the PTC is shifted away from the signal frequency. PTCs with frequency-shifted tips (indicative of dead regions) were found for all subjects. The frequencies at the tips sometimes decreased slightly with increasing signal level. For the subject with a relatively flat loss, PTCs with tips close to 3000 Hz were obtained for signal frequencies of 400, 1000 and 1500 Hz. A PTC with a tip at 5000 Hz was found for a signal frequency of 6000 Hz. These results suggest that this subject had an "island" of surviving IHCs and neurons with CFs ranging from 3000 to 5000 Hz, with extensive dead regions on either side. For the subject with a mid-frequency loss, the pattern of results suggested a mid-frequency dead region. For the subjects with high-frequency loss, the results suggested the presence of high-frequency dead regions, in one case starting at a frequency where absolute thresholds were only slightly higher than normal. PTCs can be used to detect and delimit dead regions. Often, the frequency at the tip of the PTC can be used to define approximately one boundary of the dead region. However, the detection of beats can affect the shape of the PTC around the tip when the signal frequency lies just inside the dead region. The level of the signal can also have some effect on the frequency at the tip of the PTC. Very low signal levels can lead to variable results. Dead regions can start at frequencies where absolute thresholds are near normal.

S-state Dependent FTIR Difference Spectra for Photosystem II: Examination of Proton Transfer Pathway Intermediates

Vibrational spectroscopy provides a means to investigate molecular interactions within the active site of an enzyme. We have applied difference FTIR spectroscopy coupled with a flash turnover protocol of Photosystem II (PSII) to study the oxygen-evolving complex (OEC). Our data in the mid-frequency region of 1800-1200 cm-1 show two overlapping oscillatory patterns as the sample is flashed through the four-step S-state cycle that produces O2 from two H2O molecules. The first oscillation pattern of the spectra shows a four-flash period-four oscillation and reveals a number of new vibrational modes for each S-state transition, indicative of structural changes involved in the formation of each S-state. Importantly, the first and second flash difference spectra are reproduced in the 1800-1200 cm-1 spectral region by the fifth and sixth flash difference spectra, respectively. The second oscillation pattern observed in the mid-frequency region is a four-flash period-two oscillation associated with changes primarily about the Amide I and II regions, and reports on changes in sign of the these modes that alternate 0: 0: 1: 1 during S-state advance. This four-flash period-two oscillation undergoes sign inversion that alternates during the S1-S2 and S3-(S4)-S0 transitions. The mid-frequency spectral work has also been extended below 1000 cm-1 where we will present the low-frequency S-state behavior of possible ligand modes to the Mn4Ca cluster that are 18O sensitive

TEOAE monitoring of Cisplatin induced ototoxicity in guinea pigs: the protective effect of vitamin B treatment

Objective: To evaluate Cisplatin (CP) induced ototoxicity and the effects of vitamin B treatment on ototoxicity in guinea pigs by using the Transient Evoked Otoacoustic Emission (TEOAE) technique. Methods: Eleven guinea pigs were divided into two groups and they were tested by TEOAE before and after the experiment. A TEOAE response was regarded as positive when all of the following criteria were met: 1. The mean amplitude of the cochlear response in dB pe SPL should be greater than that of the noise in the external auditory canal; 2. The reproducibility rate of the response should be greater than 50%; 3. The stimulus stability rate should be greater than 65%; 4. The signal to noise ratio of the response in 1, 2, 3, 4 and 5 kHz band frequencies should be greater than 3 dB pe SPL in at least two bands. The first group included five animals that had only CP injections. Six animals in the second group received additional 0.2 ml/kg combined vitamin B preparations for 7 consecutive days. Thereafter, the right and left ears of all animals in both groups were tested by TEOAE. Results: TEOAE responses recorded from 22 ears of 11 guinea pigs before drug administrations showed that the responses with maximum amplitude were originated from the mid-frequency region. Positive TEOAE responses were significantly reduced after CP administrations in both groups when compared with their respective pretreatment results ( P<0.01). However, vitamin B injections, in addition to a single large dose of CP, resulted in significantly better TEOAE responses than those obtained after only CP injections ( P<0.05). Conclusions: The routine use of TEOAE monitoring is recommended in clinical CP treatment protocols for the early detection and follow up of ototoxicity. Also, prospective clinical trials are needed in order to validate the protective effects of vitamin B treatment against ototoxicity.

Ethyl benzene-induced ototoxicity in rats: a dose-dependent mid-frequency hearing loss.

Rats were exposed to ethyl benzene at 0, 300, 400 and 550 ppm for 8 hours/day for 5 consecutive days. Three to six weeks after the exposure, auditory function was tested by measuring compound action potentials (CAP) in the frequency range of 1-24 kHz and 2f1-f2 distortion product otoacoustic emissions (DPOAEs) in the frequency range of 4-22.6 kHz. In addition, outer hair cell (OHC) loss was quantified by histological examination. The lowest concentration ethyl benzene had no effect on any of the above measures. At 400 ppm, auditory thresholds were increased by 15 and 16 dB at 12 and 16 kHz, respectively, and at 550 ppm by 24, 31, and 22 dB at 8, 12, and 16 kHz, respectively. DPOAE amplitude growth with stimulus level was affected only after 550 ppm at 5.6, 8, and 11.3 kHz. OHC loss was found in two of the five examined locations in the cochlea. At 400 ppm, 25% OHC loss was found at the 11- and 21-kHz region. The highest concentration evoked 40% and 75% OHC loss at the 11- and 21-kHz location, respectively. Thus, the mid-frequency region of rats is affected after exposure to relatively low concentrations of ethyl benzene (400-550 ppm). These results indicate that ethyl benzene is one of the most potent ototoxic organic solvents known today.

A HYBRID FINITE ELEMENT FORMULATION FOR MID-FREQUENCY ANALYSIS OF SYSTEMS WITH EXCITATION APPLIED ON SHORT MEMBERS

A hybrid finite element method for computing mid-frequency vibrations is presented. In the mid-frequency region a system is comprised by some members that contain several wavelengths and some members that contain a small number of wavelengths within their dimensions. The former are considered long members and they are modelled by the energy finite element analysis (EFEA). The latter are considered short and they are modelled by the finite element analysis (FEA). In this paper the excitation is considered to be applied on the short members. The hybrid formulation computes the response of the entire system. The characteristics of the long members affect the behavior of the short members and the amount of power flow between the members of the system. The resonant characteristics of the short members and the boundary conditions imposed by the long members determine the amount of input power into the system. The interaction between members is described by a set of equations between the FEA and the EFEA primary variables at the interfaces between long and short members. The equations for the short and the long members and the interface equations are solved simultaneously. A theoretical formulation and a numerical implementation for systems that contain one wave type is presented. Analytical solutions for several co-linear beam configurations are compared to numerical results produced by the hybrid finite element method. Good correlation is observed for all analyses.

Low-Level Toluene Disrupts Auditory Function in Guinea Pigs

Toluene appears to have adverse effects on the human auditory system, but it is difficult to estimate its potency since it is commonly present in the workplace in combination with noise exposure; workplace noise exposures are often highly variable. Studies designed to assess toluene ototoxicity specifically have been limited to high-dose studies in a single laboratory animal model, the rat. Here permanent hearing loss has been observed at concentrations of 1000 ppm toluene and greater after inhalation exposure for 5 days, 6 h/day. The OSHA threshold limit value for toluene is only 100 ppm. The current study focuses on the onset of toluene ototoxicity acutely in the guinea pig and in adducing a mechanism of effect. In this study, evidence is presented for the impairment of auditory function by toluene in the guinea pig, at a concentration substantially lower than that used for studying permanent impairment in the rat. The impaired function was correlated with reduced energy metabolism in outer hair cells. Assessment of auditory function was made using distortion product otoacoustic emissions (DPOAE) with subsequent measurement of succinate dehydrogenase (SDH) staining density in hair cells using surface preparations. Temporary disruption of auditory function in guinea pigs is seen in subjects exposed to 250, 500, and 1000 ppm toluene for 8 h/day, 5 day/week for 1 and 4 weeks. Concentrations as low as 250 ppm toluene were able to disrupt auditory function acutely in the guinea pig, and 500 and 1000 ppm toluene produced greater acute dysfunction. SDH staining suggests that reduced enzyme activity in the midfrequency region of the cochlea occurs acutely following toluene exposure. Although the auditory dysfunction progressed between 1 and 4 weeks of exposure, a permanent loss did not develop for these subjects and hair cell death was not seen. The current study identifies early evidence of auditory system impairment in the guinea pig at low toluene concentration and evidence for impairment of energy production in hair cells. While even a transient auditory impairment has implications for workplace safety, additional study on the transition from such acute effects to permanent impairment is essential.