Laser irradiation may have a significant impact on the microwave transmission properties of glass fiber composites. In this study, a two-dimensional microwave transmission transient model of glass fiber composites during the complete laser irradiation process was developed. The numerical results indicate that carbonization during irradiation causes a significant increase in dielectric loss, thus resulting in a decrease in transmittance. After irradiation, the dielectric loss decreased due to the decrease in temperature, which, in turn, led to an increase in transmittance. The increased transmittance after cooling was lower than the original value due to the generation of pyrolytic carbon with a high dielectric loss. The numerical results were verified by conducting an experiment wherein the microwave transmittance of glass fiber composites was measured in real time before, during, and after laser irradiation. In addition, the effects of different laser parameters on the real-time microwave transmittance during laser irradiation were investigated experimentally and numerically. For the same total energy, a laser with a high power density and short irradiation time had a more significant effect on the attenuation of transmittance.