Microplastic Size Research Articles

Microplastic contamination in commercial food and drink products and associated risk of potential human intake in Riyadh, Saudi Arabia.

Extensive production and utilization of plastics have resulted in the subsequent accumulation of microplastics (MPs) in the environment, which has become a serious threat to human health globally. Therefore, in this study, 112 drinks and food products were purchased from local markets in Riyadh, Saudi Arabia, and the abundance of MPs was investigated. The dominant size of MPs was 101-250μm for tuna fish, noodles, bottled water, and disposable containers, 251-500μm for honey, tea bags, and sugar, and 501-1000μm for salt, juice, and soft drink samples. FTIR analysis indicated polypropylene, polyethylene, polycarbonate, and polyvinylchloride as the major polymer contents. The average total number of MPs was highest in tea bags (615.71 particles teabag-1), followed by sugar (281.01 particles kg-1), honey (197.67 particles kg-1), and salt (147.30 particles kg-1). Consumption of tea bags exhibited the highest risks of daily and annual MPs intake (15.06 particles kg-1 day-1 and 5496.45 particles kg-1 year-1, respectively), followed by bottled water (4.77 particles kg-1 day-1 and 1741.32 particles kg-1 year-1, respectively). Overall, this study provides vital baseline data about MPs contamination in Saudi Arabia. These findings could be used to develop strategies to minimize MPs contamination in food and beverages. Therefore, monitoring MPs in commonly consumed dietary products to avoid adverse impacts of MPs on human health is critically important.

Spatial insights into microplastics and heavy metals levels, and risks in wastewater irrigated surface soils of Okara, Pakistan: Microplastics sizes impacts on heavy metals distribution using Structure equation model.

Pakistan's freshwater shortage and climate events have caused soil nutrient leaching, prompting cities to adopt wastewater irrigation. This study investigates wastewater as a potential source of microplastics (MPs) and heavy metals (HMs), assessing contaminant levels in wastewater-irrigated surface soils (WISS) in district Okara, Pakistan. It further explores potential ecological and human health risks associated with HMs in soil and the impact of MPs on HMs distribution and physicochemical properties. The concentrations of HM in WISS were within permissible limits (Cd: 0.48, Cu: 17.99, Zn: 29.73, Pb: 19.65, Fe: 8559.5), except for Mn, which was 182.94 mg/kg. MPs abundance ranged from 1850 to 5300 particles/kg, with the most common physical characteristics being fragment shapes (49.6%); black color (40.1%), and small sizes (<500 μm) many physical properties depicted association with HMs and MPs sizes. The structural equation model (SEM) showed a significant impact of MPs on HMs distribution, risk index, and pollution load index, but no significant relationship with physicochemical properties. The pollution indices for HMs in WISS indicated low ecological risk and negligible non-carcinogenic health risks were predicted for both adults and children. However, regular monitoring, enforcement of management and implementation plans, and remediation strategies for HMs and MPs in WISS are highly recommended.

Riverine microplastic discharge along the southern Black Sea coast of Türkiye

Abstract Rivers are critical pathways of microplastic (MP) pollution to marine environments, yet their contributions to the Black Sea remain understudied. This study evaluates the abundance and characteristics (polymer composition, shape, size, and color) of MPs discharged from 29 rivers flowing into the southern Black Sea. Using a plankton net with a 200 μm mesh size, samples were collected from river mouths, revealing an average MP abundance of 9.63±1.27 MP/m³, ranging from 1.03 to 29.8 MP/m³. Eastern Black Sea rivers exhibited significantly higher MP levels (11.0±1.57 MP/m³) compared to western rivers (5.15±1.25 MP/m³). Annual MP discharge to the Black Sea was estimated at 1.49×10¹¹ particles. Polyethylene terephthalate (PET, 59.3±2.66%), polyethylene (PE, 20.8±2.04%), and polypropylene (PP, 14.1±2.36%) were the most common polymers, with PET and PE being significantly dominant. MP sizes ranged predominantly between 200–1.000 μm, and fibers constituted the majority of shapes (64.1%), followed by fragments (28.3%). White (36.8±1.93%) and transparent (30.9±2.39%) MPs were the most prevalent colors. While no significant differences in MP characteristics were detected between basins (NMDS and ANOSIM), this study emphasizes rivers as major pathways for MP pollution in the Black Sea. These findings underscore the urgent need for targeted mitigation strategies to safeguard marine ecosystems and biodiversity.

Significance of Morphology in Characterizing Human Health Risk from di(2-ethylhexyl) Phthalate in Polyvinyl Chloride Microplastics in Groundwater

In this study, a human health risk assessment was performed on the ingestion route of groundwater containing polyvinyl chloride (PVC) microplastics (MPs), and the carcinogenic and non-carcinogenic risks of di(2-ethylhexyl) phthalate (DEHP), a representative additive, were determined. In particular, the impact of volume diversity according to the shape (morphology) of PVC MP (fragment, fiber, film) on the risk characterization was intensively explored. Firstly, a continuous particle size distribution following a power function was derived using the abundance ratio of PVC MPs by size in the investigated groundwater, and human health risk assessment for DEHP in the PVC MPs was performed through the volume distribution according to the shape of MPs. DEHP human health risk assessment showed an excess cancer risk (ECR) of below 10−6 for a 95% cumulative probability for all MP shapes, but the values varied depending on the shape. Sensitivity analysis showed that the parameter that most affected human health risk was MP volume, second to concentration, which is dependent on MP shape. Therefore, it is necessary to consider the variety of MP shapes during human health risk assessment, and it can be achieved through probabilistic risk assessment utilizing the probability distribution for size and shape of MPs.

Impact of Microplastics on Growth and Lipid Accumulation in Scenedesmus quadricauda

Microplastics (MPs), as frequent pollutants, persist in aquatic environments and have an impact on the growth and biomass production of microalgae. This study employed MPs of polyethylene (PE), polystyrene (PS), and polypropylene (PP) at concentrations of 250 mg/L with MP sizes of 50, 100, 300, and 500 µm to investigate their influences on the growth and bio-production of Scenedesmus quadricauda. The results revealed that MPs suppressed the growth of S. quadricauda and increased algal lipid production. The order of the MPs in terms of their inhibitory and lipid production effect was the following: PP &gt; PS &gt; PE. The order of their size sensitivity was 50 &gt; 100 &gt; 300 &gt; 500 µm. In the 50 µm PP culture, the inhibition of microalgal growth (inhibition rate: 49.26%) and accumulation of lipids (total lipid content: 65.40%) were most significant, especially with neutral lipid content. Additionally, scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) analyses proved that the rough MP surface led to high aggregation of microalgae, reduced the intensities of the protein-, lipid-, and carbohydrate-related bands and affected the structure of the algal cells.

Microplastics Settling in Turbid Water: Impacts of Sediments-Induced Flow Patterns on Particle Deposition Rates.

When microplastics (MPs) enter water bodies, they undergo various transport processes, including sedimentation, which can be influenced by factors such as particle size, density, and interactions with other particles. Surface waters contain suspended natural particles (e.g., clay and silt), which may impact MP settling rates. Here, we investigated how the presence of suspended sediments (SS) influenced the deposition patterns and rates of MPs in turbid waters. We systematically analyzed the settling velocities of particles, including different MP sizes and SS concentrations, in a plexiglass column with a camera array. For each experimental variant, we collected data on thousands of individual MPs, strengthening the statistical analysis of the particles' velocities. Simultaneous measurements of the SS flow and MPs trajectories revealed that the SS induced complex flow patterns, with MPs spending more time in downwelling flow regions, thereby accelerating MPs sedimentation. This effect was more pronounced when SS were aggregated. Additionally, we found that smaller MP fragments were more affected by the fluctuations than spheres or larger fragments. Collectively, our results provide valuable data for future MP fate models and help to understand the sedimentation processes of MPs in natural waters, which is crucial for assessing their environmental transport and impact.

Occurrence and Assessment of Human Exposure to Microplastic Particles in Bottled Water From Southwest, Nigeria

Background: The investigation of microplastics (MPs) in different environmental media is well documented; however, the prevalence and possible human health risks of MPs in bottled water are not fully understood. Methods: This study was designed to (a) determine the quantity, form, color, and size of MPs, (b) evaluate the influence of physicochemical properties on the release of MPs in bottled water, and (c) estimate the daily intake (EDI) of MPs in eight distinct varieties of bottled water from Southwest Nigeria. The Rose Bengal staining method and microscopic analysis were employed to identify, quantify, and characterize the MPs. Results: The bottled water brands from Southwest Nigeria contained MPs of various shapes, colors, and sizes. The average abundance of MPs was 15.2±2.9 particles/L, with the highest abundance being 21.3±1.8 particles/L. Fibers (82%) were the most detected shape of MPs, followed by fragments (18%), with transparent and blue being the two colors observed. Particle sizes ranged from 0.2 to 2.5 mm. The concentrations of MPs in the bottled water were strongly correlated (r=0.789, P&lt;0.01) with the pH of the water samples. Adults had an estimated EDI of 0.31 to 0.66 particles/kg bw/day, while children’s EDI ranged from 1.13 to 2.36 particles/kg bw/day. Conclusion: These findings provide insights for scientists to better understand the contamination of bottled water by MPs and help regulatory bodies formulate appropriate regulations for the production and bottling of drinking water.

Combined exposure to microplastics and copper elicited size-dependent uptake and toxicity responses in red swamp crayfish (Procambarus clarkia).

In recent years, the toxicity of microplastics (MPs) in combination with heavy metals, particularly the influence of varying microplastic sizes on their toxic effects, has attracted widespread attention. In this study, red swamp crayfish (Procambarus clarkia) were exposed to MPs of two particle sizes (S-MPs: 5 μm, 1 mg/L; and L-MPs: 100 μm, 1 mg/L) and Cu (5 mg/L) individually or in combination for 96 h. The accumulation patterns of MPs were as follows: gills > intestines > hepatopancreas > muscles. Moreover, the accumulation pattern of Cu was as follows: intestines > gills > hepatopancreas > muscle. Additionally, S-MPs and L-MPs enhanced Cu accumulation, with the highest levels observed in the S-MPs+Cu-treated group. Histopathological analysis showed that the combined exposure led to greater hepatopancreatic damage. Assessment of antioxidant enzymes showed decreased activities of superoxide dismutase, catalase, and glutathione among the different treatments, except for malondialdehyde, which was elevated compared to the control group. In the S-MPs+Cu-treated group, the expression levels of Cu homeostasis genes (MTF-1, ATP2, Atox1, MT) were significantly lower than those in the Cu treated group. This study provides a valuable reference for studying the combined toxic effects of MPs with varying particle sizes on heavy metals.

Disruption of midgut homeostasis by microplastics in Spodoptera frugiperda: Insights into inflammatory and oxidative mechanisms.

Microplastics have evolved as widespread contaminants in terrestrial and aquatic environments, raising significant environmental concerns due to their persistence and bioaccumulation. In this study, we investigated the toxicity of polyethylene microplastics (PE-MPs) on the agricultural insect, Spodoptera frugiperda. Maize leaves containing three sizes (0.5 μm, 5 μm, and 50 μm) of PE-MPs were fed to fall armyworm larvae for 12 days at concentrations of 1.25 g/ L, 5 g/L, and 20 g/L. The results showed that smaller size and higher concentration of microplastics led to increased toxicity. Furthermore, different sizes and maximum concentrations of PE-MPs were selected for subsequent experiments to observe changes in histological and enzymatic biomarkers, midgut microbiome, and metabolic responses. Following PE-MPs exposure, inflammation signs and oxidative stress were detected in the midgut. Significant changes were also observed in midgut microbiota and metabolomes, most related with oxidative stress, inflammatory disorders, and energy metabolism. These results provide evidence of midgut damage and alterations in the microbiota and metabolome of S. frugiperda because of PE-MPs exposure, highlighting the harm that microplastics can inflict on agricultural insects. Additionally, the study lays a theoretical foundation for future research on the transmission of microplastics through the food chain in agricultural ecosystems.

Identification of Characteristics, Abundance, Color, and Size of Microplastics in Shellfish in the Coastal Waters of Galesong Beach

The increasing presence of microplastics in the waters has been known to have the potential to enter various types of marine biota including shellfish. the type of research conducted was descriptive observational research) The research location was the coastal area of North Galesong Beach, Takalar Regency. The results showed that the average abundance of microplastics in mussels in Galesong Waters was 4,258 items/individual. The average (mean) intake rate of respondents was 185.86 gr/day, the median value was 167 gr/day. The minimum intake rate was 115 gr/day and the maximum intake rate was 300 gr/day. The mean exposure frequency of respondents was 126 days, the median was 104 days. The minimum exposure frequency was 54 days and the maximum exposure frequency was 255 gr/day. the types of microplastics found were all in the form of lines, fragments with red, blue and black colors. The size found was between 0.505-10.488 mm. The most common type of microplastics found in both sediments and mussels is the fragment form, followed by lines with a variety of colors, namely red, blue and black, there are differences in microplastics abundance between the two types of mussels, but differences in abundance can be seen between research stations as mussel habitat.

New Insights in Microplastic Cellular Uptake Through a Cell-Based Organotypic Rainbow-Trout (Oncorhynchus mykiss) Intestinal Platform.

Microplastics (MPs) in fish can cross the intestinal barrier and are often bioaccumulated in several tissues, causing adverse effects. While the impacts of MPs on fish are well documented, the mechanisms of their cellular internalization remain unclear. A rainbow-trout (Oncorhynchus mykiss) intestinal platform, comprising proximal and distal intestinal epithelial cells cultured on an Alvetex scaffold, was exposed to 50 mg/L of MPs (size 1-5 µm) for 2, 4, and 6 h. MP uptake was faster in RTpi-MI compared to RTdi-MI. Exposure to microplastics compromised the cellular barrier integrity by disrupting the tight-junction protein zonula occludens-1, inducing significant decreases in the transepithelial-electrical-resistance (TEER) values. Consequently, MPs were internalized by cultured epithelial cells and fibroblasts. The expression of genes related to endocytosis (cltca, cav1), macropinocytosis (rac1), and tight junctions' formation (oclna, cldn3a, ZO-1) was analyzed. No significant differences were observed in cltca, oclna, and cldn3a expression, while an upregulation of cav1, rac1, and ZO-1 genes was detected, suggesting macropinocytosis as the route of internalization, since also cav1 and ZO-1 are indirectly related to this mechanism. The obtained results are consistent with data previously reported in vivo, confirming its validity for identifying MP internalization pathways. This could help to develop strategies to mitigate MP absorption through ingestion.

Machine learning-based prediction and model interpretability analysis for algal growth affected by microplastics.

Microplastics (MPs), the plastic debris smaller than 5mm, are ubiquitous in waterbodies and have been shown to be toxic to aquatic organisms, especially to microalgae. The aim of this study is to use machine learning models to predict the effects of MPs on algal growth and to evaluate the relative importance of different features (MP properties, algal characteristics, and experimental conditions) through model interpretability analysis. Based on literature search, 408 samples were collected as inputs for the models. Three integrated machine learning algorithms, Random Forest (RF), Categorical Boosting (CatBoost), and Light Gradient Boosting Machine (LightGBM), were used to construct classification prediction models for algal growth. Our results show that the LightGBM model yields the best performance, with a total accuracy rate of 0.8305 and a Kappa value of 0.7165. The model interpretability analysis indicates that "Exposure time", "MP concentrations", and "MP size" are the most influential features affecting algal growth. For "Exposure time", which belongs to experimental conditions, 72-216h of MP exposure was found to exert the greatest effects on algal growth. The impact of MPs on algal growth increases with increasing MP concentrations over the range of 0 to 300mg/L. Smaller MPs exert more effects on algal growth, and MPs are more likely to inhibit algal growth when the ratio of algal cell size to MP size is higher. Our study successfully established prediction models for evaluating the effects of various MP properties on algal growth. This study also provides insights into the prediction of MP toxicity in organisms.

Concentration‐ and Size‐Dependent Influences of Microplastics on Soil Hydraulic Properties and Water Flow

ABSTRACTExtensive usage of agricultural plastic film correspondingly leads to excessive residues of microplastics (MPs). MP accumulation alters soil hydraulic properties and water flow. However, little is known about the combined effects of concentration and particle size on soil hydrological properties, and a numerical approach for modelling infiltrated flow has not been well developed. Hence, we determined soil hydraulic properties and infiltrated flow affected by MP concentration and particle size and established a water flow model suitable for MP‐contaminated soils. Quantitative findings indicated that the saturated conductivity for soil–MP mixture was 10.8%–50.0% smaller than that for pure soil, which decreased and increased with the increase in MP concentration and size, respectively. The MP concentration always had significant influences on saturated conductivity; in contrast, the MP particle size always generated significant influences under the condition of small particle size. Besides, higher concentration or size of MPs led to weaker soil water‐holding capacity, and the saturated and residual water content decreased by 0.6% – 41.5% and 0.2% – 11.6%, respectively. Furthermore, the presence of MPs inhibited water infiltration, with the wetting front migration rate and cumulative infiltration decreased by 7.1% – 29.4% and 4.7% – 21.7%, respectively, with the increase in the MP concentration and size. Correlation analysis indicated that MP particle size was negatively correlated with saturated/residual moisture, wetting front migration and cumulative infiltration; in addition, MP concentration was negatively correlated with saturated conductivity, residual moisture, wetting front migration and cumulative infiltration; compared with the MP particle size (15.63%), the MP concentration (46.28%) played a major role in the response of soil hydraulic properties and water movement to changes in the external environment. A two‐dimensional numerical approach was proposed by considering the Richards equation and hydraulic parameters of soil–MP mixture, and a model based on finite element theory was further employed and validated through comparing experimental observations with numerical simulations, which indicated that the proposed model had a high accuracy in simulating the infiltration process in MP‐contained soils. Our findings elucidate the influence of MP concentration and size on soil hydraulic properties and water flow and confirm the potential of using simulations to predict water infiltration in MP‐contained soils.

The role of human intestinal mucus in the prevention of microplastic uptake and cell damage.

An increase in plastic waste and its release into the environment has led to health concerns over microplastics (MPs) in the environment. The intestinal mucosal layer is a key defense mechanism against ingested MPs, preventing the migration of particles to other parts of the body. MP migration through intestinal mucus is challenging to study due to difficulties in obtaining intact mucus layers for testing and numerous formulations, shapes, and sizes of microplastics. Previous studies have primarily used mucus from animals, hydrogel models, and mucus samples from other parts of the body as substitutes. This study examines how different MP compositions, sizes (40-500 nm), and surface functionalizations alter MP migration through human intestinal mucus; how the mucus layer protects cells from MP uptake, toxicity, and inflammation; and how the intestinal mucus prevents the migration of other environmental toxins via MP particles. The presence of a mucus layer also provides critical protection against cytotoxicity, reactive oxygen species production, and uptake for all particles tested, although certain functionalizations, such as streptavidin, are particularly harmful to cells with high toxicity and inflammation. Understanding the properties that assist of impede the diffusion of MPs through mucus is relevant to the overall bioaccumulation and health effects of MPs as well as drug delivery purposes.

Genes of filter-feeding species as a potential toolkit for monitoring microplastic impacts.

Microplastics (MPs) are ubiquitous in the marine environment and impact organisms at multiple levels. Understanding their actual effects on wild populations is urgently needed. This study develops a toolkit to monitor changes in gene expression induced by MPs in natural environments, focusing on filter-feeding and bioindicator species from diverse ecological and taxonomic groups. Six candidate genes -Caspase, HSP70, HSP90, PK, SOD, and VTG- and nine filter-feeding species -two branchiopods, one copepod, five bivalves and one fish- were selected based on differential expression in response to MPs exposure (mainly the widely used polystyrene and polyethylene polymers) reported in over 30 publications. Some genes are particularly determinant, such as HSP70 and HSP90 (key to managing a wide range of stressors) and SOD (critical for addressing oxidative stress), as they are more directly related to stress. PK is related to carbohydrate metabolism (alterations in energy metabolism); VTG is associated with reproductive problems; Caspase mediates in apoptosis. Each gene in the toolkit plays a role depending on the type of stress assessed, and their combination provides a comprehensive understanding of the impacts of MPs. Differences in gene expressions between species and the exposure thresholds were found. These genes were examined in various scenarios with different types, concentrations, and sizes of MPs, alone or with other stressors. The toolkit offers significant advantages, allowing a comprehensive study of the impact of MPs and focusing on filtering bioindicator species, thus enabling pollution assessment and long-term monitoring. It will outperform traditional methods like tissue counts of MPs where only physical damage is visible, providing a deeper understanding. To our knowledge, this is the first toolkit of its kind.

Effect of microplastics on soil greenhouse gas emissions: A global meta-analysis study.

Microplastics (MPs) emerged as a critical global pollutant, yet their effects on soil greenhouse gas (GHG) emissions remain uncertain. This meta-analysis evaluates the effects of MPs exposure on GHG emissions and identifies key influencing factors. Regardless of any influencing factors, MPs exposure decreased N2O emissions by 28.5%, while increased CO2 and CH4 emissions by 0.07% and 28.6%, respectively. However, these changes were statistically insignificant. The factors such as MPs concentration, shape, and type, initial soil type and pH, and the presence of additional additive were identified to significantly influence N2O emission response. The most substantial increase in N2O emissions occurred when MPs exposed to silt soils (+55.8%), whereas the greatest inhibition was observed in soils with fertilizer addition (-48.8%). CO2 emission response was significantly influenced by MPs size and shape, initial soil pH and type, experimental duration, and co-additives, with the MPs exposure in sand soils exhibiting the highest increase (+20.7%) and the exposure of fiber MPs causing the largest reduction (-40.4%). Additionally, MPs shape and initial soil pH and type were found to significantly affect CH4 emission response. The model selection analysis revealed that the response ratio [ln (RR)] of nirS gene to MPs exposure and MPs concentration were the most critical factors influencing N2O emissions, whereas the size, concentration, and type of MPs, ln (RR) of Chitinase, initial soil pH, additional additive, and experimental duration were the most important influencing factors for CO2 emissions. Overall, this study highlights the high uncertainty associated with the response of GHG emission to MPs exposure due to the complex interplay of abiotic and biotic processes mediated by MPs under varying conditions. In the future, extensive studies across diverse conditions of MPs (e.g., type, shape, and concentration) and soil (e.g., texture and pH) are still urgently needed.

Impact of Heterosigma akashiwo on the environmental behavior of microplastics: Aggregation, sinking, and resuspension dynamics.

Aggregation processes of microalgae have significant effects on the vertical distribution of microplastics (MPs) in the marine environment. This study explored how the harmful microalga Heterosigma akashiwo affects the aggregation and sinking characteristics of four types of MPs: low and high-density polyethylene (PE) spheres, and small and large polypropylene (PP) fragments. The aggregation of MPs was primarily driven by extracellular polymeric substances (EPS) rather than direct attachment to the cells, contributing to their sinking. The sinking of low-density PE spheres followed a logistic function, saturating at 28 % with a half-saturation time of 9 days. In contrast, small PP fragments sank minimally (under 2 %) and large PP fragments showed almost no sinking, indicating the varying impacts of MP density and size. The sinking velocity of the MP aggregates was significantly lower for low-density PE spheres (0.63 mm∙s-1) than for high-density PE spheres (0.81 mm∙s-1), despite no significant differences in aggregate size or MP particle number. This result may suggest that low-density MPs could potentially affect marine carbon cycle. Furthermore, no clear evidence was found for the resuspension of the settled aggregates due to bacterial decomposition under dark and cold conditions. As the first experimental study to explore the aggregation, sinking, and resuspension of different MPs in the presence of H. akashiwo, these findings, when integrated with field observations and modeling studies, provide valuable insights for predicting MP distribution in marine environments.

Abundance of microplastics in a typical urban wetland in China: Association with occurrence and carbon storage.

Microplastics (MPs) are gaining attention for their widespread presence and toxicity in ecosystems. However, their role as a carbon source in urban wetland carbon sinks is still unclear. In this study, the microplastic-carbon (MP-C) was firstly quantified based on the abundance and occurrence characteristics, including MP morphology, size and type in the Sanyang Wetland, a typical urban wetland of China. MP abundances ranged from 2.4 ± 0.6-14.9 ± 1.5 items/L in surface water and 6.6 ± 1.2 × 103 to 46.3 ± 5.9 × 103 items/kg in sediment. The predominant morphological characterization of MPs was fragments smaller than 200 μm in size, consisting of PP, PE, and PET, which suggests that the main source was domestic wastewater discharge nearby. Notably, in the Sanyang wetland, the contribution of MP-C to total organic carbon (TOC) was estimated to be 0.0230.20 % in water and 0.0260.28 % in sediment. With the continuous production of plastics globally, these values were predicted to increase to 0.12 0.71 % and 0.83 4.12 % by 2100, respectively. Although the estimations relied on simplified geometric assumptions for MP volume and theoretical carbon content, these approaches provide a reasonable basis for understanding MP-C dynamics in wetlands under current analytical constraints. The integration of MP-C characterization during environmental monitoring and management strategies would enhance our understanding of MP pollution's role in the carbon cycle.

Microplastics in Landfill Environments: Distribution, Characteristics, and Risks from Gampong Jawa, Indonesia

Landfills are generally considered the ultimate solution for waste management. However, the degradation process of plastic waste in landfills causes the release of microplastic particles into the surrounding environment and threatens human health. The distribution and properties of microplastics in four environment matrices, soil, leachate, river water, and well water surrounding the landfill, are examined in this study. Sampling was conducted at the inflow and outflow areas of the leachate ponds., The soil at the top (0–5 cm) and bottom (5–20 cm), upstream and downstream surface water adjacent to the landfill, and community wells within a radius of fewer than 700 meters from the landfill. Microplastic analysis used a gradual extraction method with saturated NaCl for density separation, 30% hydrogen peroxide for organic matter degradation, and 0.05 M FeSO4 as a catalyst. Physical character identification of microplastics using a microscope showed microplastic contamination at all study sites. The results showed an abundance of microplastics was found in well water samples (808 to 979 items/L), leachate (209 to 757 items/L), surface water (6.29 to 7.2 items/L), and soil (23,340 to 23,420 items/kg). Types of microplastics found consist of fragments, fibers, films, pellets, foam, and rods. The size of microplastics found ranged from 1.897 µm to 1,642.79 µm. Fourier Transform Infrared spectroscopy examination identified polyethylene terephthalate (PET) plastic compounds in soil and leachate materials. The high concentration of microplastics in well water indicates potential groundwater contamination from landfill activities that may impact the surrounding community. This study provides preliminary insights into how landfills may contribute to environmental microplastic contamination. It paves the way for further research to develop mitigation strategies.

MicroMetaSense: Coupling Plasmonic Metasurfaces with Fluorescence for Enhanced Detection of Microplastics in Real Samples.

Diverse analytical techniques are employed to scrutinize microplastics (MPs)─pervasive at hazardous concentrations across diverse sources ranging from water reservoirs to consumable substances. The limitations inherent in existing methods, such as their diminished detection capacities, render them inadequate for analyzing MPs of diminutive dimensions (microplastics: 1-5 μm; nanoplastics: < 1 μm). Consequently, there is an imperative need to devise methodologies that afford improved sensitivity and lower detection limits for analyzing these pollutants. In this study, we introduce a holistic strategy, i.e., MicroMetaSense, reliant on a metal-enhanced fluorescence (MEF) phenomenon in detecting a myriad size and types of MPs (i.e., poly(methyl methacrylate) (PMMA) and poly(ethylene terephthalate) (PET)) down to 183-205 fg, as well as validated the system with real samples (tap and lake) and artificial ocean samples as a real-world scenario. To obtain precise size distribution in nanometer scale, MPs are initially processed with an ultrafiltration on-a-chip method, and subsequently, the MPs stained with Nile Red dye are subjected to meticulous analysis under a fluorescence microscope, utilizing both a conventional method (glass substrate) and the MicroMetaSense platform. Our approach employs a metasurface to augment fluorescence signals, leveraging the MEF phenomenon, and it demonstrates an enhancement rate of 36.56-fold in detecting MPs compared to the standardized protocols. This low-cost ($2), time-saving (under 30 min), and highly sensitive (183-205 femtogram) strategy presents a promising method for precise size distribution and notable improvements in detection efficacy not only for laboratory samples but also in real environmental samples; hence, signifying a pivotal advancement in conventional methodologies in MP detection.