Use Of Microorganisms Research Articles

Efecto de fertilización mixta en cultivo de maíz (Zea mays L.), en Tehuacán Puebla, México

The objective of this research was to evaluate the effect of mixed fertilization on maize cultivation, considering the application of Glomus intraradices and Azospirillum brasilense inoculated into the seeds of the native white Tehuacanero maize as biological fertilization. Chemical fertilization was also applied using the 90-90-46 formula, with urea, triple superphosphate, and potassium chloride as nutrient sources. The experimental design used was a randomized complete block design, evaluating 9 treatments with 4 replications. The treatment results showed a significant effect (p < 0.027), where treatments with mycorrhizae, Azospirillum, or their combinations had a positive impact on grain yield compared to the control treatment, which received neither fertilization nor inoculation. These findings suggest that the use of microorganisms can reduce dependence on chemical fertilizers, contributing to more sustainable and environmentally friendly agricultural practices.

The family Thermoactinomycetaceae: an emerging microbial resource with high application value

In recent years, interest has increased in the use of microorganisms to obtain additional valuable resources for green and sustainable development. Preliminary functional analyses have indicated that members of the family Thermoactinomycetaceae have good application potential for the production of novel specific enzymes, high-value bioactive compounds, novel secondary metabolites and the promotion of efficient biomass conversion. Therefore, they can be considered a new class of microbial resources with potentially high value. However, the lack of culture and culture-independent techniques coupled with the uncertain taxonomic status of the family Thermoactinomycetaceae, has made exploring these potential applications challenging. This paper reviews the distribution characteristics and functional properties of the family Thermoactinomycetaceae, providing a detailed interpretation of the potential application value of this group and proposing a set of systematic resource development strategies based on a combination of culture-dependent and culture-independent strategies to exploit its potential for resource applications.

Influence of Rhodococcus qingshengii VKM Ac-2784D biostimulator on bacterial isolates from potato endo- and rhizosphere

The potato microbiome has a significant impact on plant growth and development. In order to affect this microbiome, agriculture can use various biopreparations on the basis of soil microorganisms. Being vulnerable to pathogens and drought, potato plants are particularly useful in the development of biopreparations. Special attention is given to Rhodococcus bacteria due to their ability to clean contaminated soil and stimulate plant growth. The present study was aimed at examining the effect of Rhodococcus qingshengii VKM Ac-2784D on bacteria isolated from potato endo- and rhizosphere. It is known that only a small fraction of microorganisms within the plant microbiome can be obtained in pure culture. Given these limitations, it was possible to isolate over 70 endophytic strains without the use of selective media and show that many of them are sensitive to the presence of a biopreparation component on the basis of Rhodococcus qingshengii VKM Ac-2784D. The metagenomic study indicates a change in the composition of the microbial community following treatment with the biopreparation. The experiments also show that the bacteria remain sensitive to Rhodococcus even in the presence of other competing strains. In general, the study results indicate a modulating effect of the biopreparation on the potato microbiome without phytotoxicity. The findings are important for understanding the effect of the biopreparation on the microbial composition of soil and potato plants, as well as for developing effective strategies for the use of microorganisms in agriculture.

Bacteria applied to cement as an alternative against cracking in buildings: a systematic literature review

This research aims to carry out a comprehensive review of the scientific and technical literature related to the application of bacteria as an innovative and sustainable alternative to prevent and repair cracks in concrete structures used in real estate. The problem of cracks in concrete constructions is widely recognised as a critical challenge in the construction industry, with implications for structural integrity, aesthetics and maintenance costs. In the context of this study, emphasis will be placed on identifying the main species of bacteria with potential application in construction, as well as investigating the biotechnological mechanisms underlying the healing of cracks by these microorganisms. The production of calcium carbonate by the bacteria, which fills cracks and strengthens the concrete matrix, will be addressed in detail. In addition, case studies illustrating the effectiveness of bacteria in different environmental conditions and concrete compositions will be analysed, providing a comprehensive overview of the practical applications of this approach. Safety and regulatory aspects involving the use of microorganisms in construction environments will also be considered. This study seeks to contribute to the advancement of knowledge on the biological healing of cracks in concrete structures, highlighting its potential to improve the durability of buildings, reduce maintenance costs and promote sustainable construction practices. It is hoped that the results of this systematic literature review will provide valuable lessons for researchers, construction professionals and others interested in the field of civil engineering and biotechnology applied to construction.

Is biomining a feasible solution for E-waste management?

With global growth in electronic waste (e-waste) at staggering rates, traditional methods of recycling pose significant health and ecological risks due to the use of energy intensive process and hazardous chemicals used. The use of microorganisms to extract valuable metals from waste, called biomining, is a promising alternative to conventional e‐waste management practices. This study critically examined the feasibility of biomining as a sustainable alternative to recycling of e-waste. By comparing biomining with traditional methods, this study explored its environmental benefits, reduced chemical usage, and lower energy consumption, and its limitations, slower recovery rates and complex microbial processes. The study showed that biomining is most efficient when it is used to recover valuable metals such as gold and copper, but is less likely to be as efficient in the recovery of low-grade materials. In addition, it discussed the conditions under which it is conceivable that biomining is a sound solution and points out the importance of microbial optimization and appropriate economic and environmental conditions. Finally, the substantial areas for future research are presented, such as developing more efficient biomining processes, performing long term environmental impact assessment and life cycle analysis to assess the sustainability aspects of biomining as an e-waste management route.

Response of Phaseolus vulgaris to the Use of Growth-Promoting Microorganisms Associated with the Reduction of NPK Fertilization in Tropical Soils: Clayey Oxisol and Sandy Ultisol

This research analyzed the effects of inoculation with Rhizobium tropici, combined with beneficial microorganisms, on the reduction of NPK fertilization and its impacts on common beans. Conducted in two types of soil (clayey Oxisol and sandy Ultisol), the experimental design was a randomized block design under a factorial scheme 4 × 4, with different combinations of inoculation (Rhizobium tropici alone or in combination with Azospirillum brasilense, Bacillus subtilis, and Trichoderma harzianum) and fertilization doses (0%, 33%, 66%, and 100% of the recommended dose). The results showed that inoculation with R. tropici, especially in combination with Trichoderma harzianum, increased nodule formation and improved agronomic parameters such as leaf chlorophyll, dry matter of the plant, number of pods, and grains. Co-inoculation with Azospirillum brasilense resulted in a significant increase in grain yield, particularly in clayey soil with 33% NPK. Inoculation with lower doses of NPK was sufficient for good yield, suggesting the feasibility of reducing the use of mineral fertilizers. This study indicates that soils with corrected fertility, in a no-tillage system, can contribute to the reduction of fertilizer use due to the cycling of organic matter and improvement of soil health. Additionally, the use of microorganisms is an effective ally for the sustainability of agroecosystems.

Exploring Novel Fungal-Bacterial Consortia for Enhanced Petroleum Hydrocarbon Degradation.

Bioremediation, involving the strategic use of microorganisms, has proven to be a cost-effective alternative for restoring areas impacted by persistent contaminants such as polycyclic aromatic hydrocarbons (PAHs). In this context, the aim of this study was to explore hydrocarbon-degrading microbial consortia by prospecting native species from soils contaminated with blends of diesel and biodiesel (20% biodiesel/80% diesel). After enrichment in a minimal medium containing diesel oil as the sole carbon source and based on 16S rRNA, Calmodulin and β-tubulin gene sequencing, seven fungi and 12 bacteria were identified. The drop collapse test indicated that all fungal and four bacterial strains were capable of producing biosurfactants with a surface tension reduction of ≥20%. Quantitative analysis of extracellular laccase production revealed superior enzyme activity among the bacterial strains, particularly for Stenotrophomonas maltophilia P05R11. Following antagonistic testing, four compatible consortia were formulated. The degradation analysis of PAHs and TPH (C5-C40) present in diesel oil revealed a significantly higher degradation capacity for the consortia compared to isolated strains. The best results were observed for a mixed bacterial-fungal consortium, composed of Trichoderma koningiopsis P05R2, Serratia marcescens P10R19 and Burkholderia cepacia P05R9, with a degradation spectrum of ≥91% for all eleven PAHs analyzed, removing 93.61% of total PAHs, and 93.52% of TPH (C5-C40). Furthermore, this study presents the first report of T. koningiopsis as a candidate for bioremediation of petroleum hydrocarbons.

Cellulolytic potential of new strains of soil streptomycetes

In order to obtain new strains of cellulolytic microorganisms for use in technologies for processing plant and other cellulose-containing waste, four new bacterial strains of the genus Streptomyces have been isolated from various sources. In tests with Congo red, the isolates’ ability to degrade carboxymethylcellulose (CMC), microcrystalline cellulose (MCC) and natural cellulose–containing materials – straw, birch and oak sawdust was determined. The activity of cellulase was quantified during fermentation of straw. On media with each of the model carbon sources, the radial growth rate was determined as an indicator characterizing the ability of isolates to colonize various substrates. The conjugate characteristic of cellulase activity and radial growth rate on substrates of various natures made it possible to assess the prospects for further use of the studied strains as destructors of cellulose-containing waste. The strain Streptomyces thermocarboxydus T1-3 was the leader in the studied sample, characterized by the most active enzymatic hydrolysis of all model cellulose sources, a high radial growth rate (up to 87±3 microns/hour), and the achievement of maximum cellulase activity (171.25±8.13 U/ml) during straw fermentation within 24 hours.

Transforming Soil Stability: A Review on Harnessing Plant Cell Compounds and Microbial Products for Modifying Cation Exchange Capacity

Soil stabilization is a very important method of science and engineering for improving the properties of soil. This paper aims to stabilize expansive black cotton soil through a biological approach involving plant extracts, plant waste materials, and microorganisms. While chemical methods exist, i.e., lime stabilization, geotextiles, etc., they are not economically feasible for large-scale applications. The primary issue with black cotton soil is due to the presence of montmorillonite clay mineral, which makes it unsuitable for the construction of roads and airfields. The cation exchange capacity (CEC) can be defined as the ability of soil to absorb and exchange positively charged ions; thus, if free positively charged ions are not available, the soil will not exchange them with others. The CEC of the soil is diminished, and ultimately, the soil is stabilized to some extent. This paper explores the preparation of plant extract, which contains a high number of anions, and directly inoculates it with soil, which nullifies the positive charge of the soil and diminishes the CEC. The use of cellulose and lignin-degrading microorganisms as an energy source and other minerals that are needed for their growth will be utilized from the soil to reduce CEC, i.e., Mg required for DNA replication and Ca required for their growth and maintenance. Another approach to diminishing the CEC is to use the microorganisms that produce EPS, which require Ca and Mg as adhesions for the formation of biofilm, i.e., Pseudomonas aeruginosa, Bacillus subtilis, and Escherichia coli. The use of microorganisms that have specific enzymes is also used in the diminishing soil CEC, i.e., by using ureolytic enzyme-producing bacteria like Sporosarcina pasteurii, Bacillus paramycoides, Citrobacter sedlakii, and Enterobacter bugadensis.

An Oral Microbiome Perspective: Postbiotics as a Prospective Therapeutic Agents in Dental Medicine (A Short Communication)

Recently, there has been an increase in interest in the use of microorganisms that are used as a preventive effort to prevent dental and oral health problems, known as probiotics, prebiotics, synbiotics, and postbiotics. As more and more clinical trials of the use of microorganisms show positive results for certain conditions, Postbiotics have several advantages, including safety, longer shelf life, absence of risk and patentability, which makes them more attractive than probiotics. This review covers a wide range of aspects, starting with an introduction, terminology, role of post biotik, and a brief mechanism of action. The discovery section concludes by highlighting the challenges and future directions to improve postbiotic discovery. The review concludes by highlighting the ongoing applications of postbiotics in expanded clinical trials, providing a comprehensive overview of the current landscape in postbiotic development

Complete genome sequence of Bacillus pumilus NWMCC0302, a strain for degrading bovine blood

BackgroundDirectly discharging livestock and poultry slaughter blood without proper treatment can cause severe ecological damage. Exploring the use of microorganisms to break down waste blood into smaller molecules such as peptides and amino acids, as well as investigating the possibility of transforming these small molecules into water-soluble fertilizers containing amino acids, holds significant research value in the comprehensive utilization of livestock and poultry blood.ResultsIn this study, a single strain of Bacillus pumilus NWMCC0302, which has effectively degraded bovine blood, was isolated from abattoir blood sludge using casein agar plates and Columbia blood agar plates. The degradation test was carried out using bovine raw blood as a nitrogen source in the medium, and the results showed that the strain degraded 53.83% of bovine blood under optimal degradation conditions. The whole genome sequencing of Bacillus pumilus NWMCC0302 was conducted using the second-generation DNBSEQ platform and the third-generation PacBio platform, employing high-throughput sequencing technology. The size of the strain's entire genome was determined to be 3 868 814 bp with a G-C content of 41.63%. The total gene length accounted for 88.98% of the genome length at 3 442 341 bp and encoded 4 113 genes. The strain contained 79 tRNAs, 24 rRNAs, 7 sRNAs, and 296 repetitive sequences. The gene data obtained from sequencing were also functionally annotated using the COG, KEGG, and VFDB databases. In the COG database, 310 genes were involved in amino acid transport and metabolism, including 10 catabolic proteins related to COGs. In the KEGG database, were 201 genes involved in amino acid metabolism pathways, including 8 genes in nitrogen metabolism pathways and 2 genes in oxidative pathways. The VFDB database contains two lysostaphin genes and one serine protease-hydrolyzed ClpP gene.ConclusionsIn summary, Bacillus pumilus NWMCC0302 was screened for its efficient ability to degrade bovine blood. Additionally, the genetic information of Bacillus pumilus NWMCC0302 was revealed at the genetic level, providing a feasible experimental method for applying this strain to the degradation of blood from slaughtered livestock and poultry. Moreover, it is a potential functional strain for producing amino acid-containing water-soluble fertilisers.

Effect of organic farming on root microbiota, seed production and pathogen resistance in winter wheat fields

Societal Impact StatementAgricultural intensification is a major driver of biodiversity decline in agrosystems. For instance, it has been shown that conventional farming leads to a decline in soil microbial diversity and triggers a strong selection process, altering the functioning of the whole ecosystem. The present study shows that organic farming increases diversity and affects composition of crop plant microbiota, mostly as a response to field management and soil characteristics. Furthermore, crop plant microbiota influences crop production and resistance to pathogens. Therefore, agricultural practices affect plant performance through microorganism‐mediated changes, which may be important pillars of future sustainable crop production.Summary Agricultural intensification threatens biodiversity, but the effects of intensification on microorganisms are still overlooked despite their role in ecosystem functioning. Microorganisms associated with plants provide many services that affect plant growth and health. Organic farming is expected to strongly affect species composition, richness, and their interactions. We analyzed the effect of the farming system on endophytic microbial assemblages associated with winter wheat plants and plant performance in the field. We collected environmental data through farmer interviews, soil analyses, and plant inventories and analyzed root microbiota at vegetative and flowering stages. Organic farming increased fungal and bacterial diversity associated to wheat plants and affected species composition in most phyla. This effect was mostly due to soil characteristics and field management and a little to plant diversity in the field. Microbial responses were more pronounced at the late developmental stage, likely as a result of accumulative effect of management actions during plant development. Seed production and resistance to pathogens were related to specific phyla that are important for seed production and/or wheat resistance to septoriose. This work advances our understanding of how agricultural practices affect plant performance through microorganism‐mediated changes and supports the use of microorganisms as pillars of sustainable crop production.

Isolation and assessment of highly sucrose-tolerant yeast strains for honey processing factory’s effluent treatment

Wastewater from many food and beverage manufacturers is enriched in organic content, and it must therefore be treated before being discharged to comply with the strict environmental regulations to protect the final water quality. Concretely, the honey processing wastewater, that remains in holding tanks until is disposal, is a rich source of sugars and this high level of organic material will degrade the water quality if not treated properly provoking an imbalance in the ecosystem. There are different strategies for an adequate treatment of this wastewater effluent to obtain a sustainable usage. One of the techniques that is more cost-effective and environmental friendlier than chemical procedures used for water remediation, is the use of microorganisms (including algae, fungi, yeasts, or bacteria). Given that they are fast-growing, robust, and metabolically diverse, yeast strains are often used for wastewater treatment. In this work, we have studied the potential for bioremediation of non Saccharomyces yeast isolated from a honey processing wastewater generated by an Argentine exporting company. The inoculation of these yeast strains to the existing flora in the honey wastewater yielded a better improvement in the treatment yield. These results suggest that these strains display a promising role could for optimizing bioremediation strategies in industrial wastewater treatment processes.

Synthesis of Nanoparticle ZnO via Chemical Reduction Using Singkil (<i>Premna serratifolia linn</i>) Leaf Extract as Photocatalytic

Nanoparticles are one of the widely studied research topics. ZnO nanoparticles have numerous benefits, such as photocatalysts and antibacterial applications. Methylene blue, which a highly dangerous and pollutes the environment and human health, is mostly used as a coloring dye in the textile industry. The use of biodegradation to treat textile waste is time-consuming and less effective. Applying photocatalysts using semiconductor materials is a more efficient method than conventional approaches for decomposing organic waste. One environmentally friendly method is green synthesis, which involves the use of microorganisms, enzymes, and plant extracts in the fabrication process. In this study, the green synthesis using chemical reduction of Premna serratifolia linn leaf extract was used to produce ZnO nanoparticles. The objective of this study is to investigate the effect of temperature on the fabrication of ZnO nanoparticles and their photocatalytic performance. Zinc nitrate tetrahydrate was used as a precursor, and the furnace temperature was varied at 400, 500, and 600 °C. The obtained ZnO was then tested using scanning electron microscopy (SEM), X-ray diffractometry (XRD), and Fourier transforms infrared spectrophotometry (FTIR). Moreover, the photocatalytic test was evaluated by examining the degradation efficiency of methylene blue using UV light. The XRD analysis indicated that the ZnO nanoparticles had crystallite sizes ranging between 44-60 nm. The SEM morphological test showed that the ZnO particles had a nano-sized spherical shape. The FTIR test results demonstrated the presence of ZnO peaks around 520 cm‑1. The performance of photocatalytic activity under UV light irradiation was significantly affected by tuning the temperatures. It was observed that the photocatalytic activity increased with increasing temperature, and methylene blue degradation efficiency reached 50% at a temperature of 600 °C. The ability of ZnO as a photocatalyst material was also evaluated by recycling the used material two times, where there was no significant change in photocatalytic performance.

Exploring biodegradable alternatives: microorganism-mediated plastic degradation and environmental policies for sustainable plastic management.

Plastics offer versatility, durability and low production costs, but they also pose environmental and health risks due to improper disposal, accumulation in water bodies, low recycling rates and temporal action that causes physicochemical changes in plastics and the release of toxic products to animal health and nature. Some microorganisms may play crucial roles in improving plastic waste management in the future. Cunningamella echinulata has been identified as a promising candidate that remains viable for long periods and produces a cutinase that is capable of degrading plastic. Other recent approaches involving the use of microorganisms are discussed in this review. However, there does not seem to be a single science that is efficient or most appropriate for solving the problem of plastic pollution on the planet at present. Regulations, especially the implementation of different laws that address the entire plastic cycle in different countries, such as Brazil, the USA, China and the European Union, play important roles in the management of this waste and can contribute to reducing this problem. In the context of the transversality of the information compiled here, the current limitations are discussed, and an effective plan is proposed to reduce plastic pollution. Although it is challenging, it involves implementing legislation, promoting sustainable alternatives, improving collection and recycling systems, encouraging reuse, supporting research and technological innovation, promoting corporate responsibility, collaborating globally, raising public awareness, optimizing waste management and, above all, continuously monitoring the progress of actions based on measurable metrics.

Separation and purification of antimicrobial substances from Paenibacillus polymyxa KH-19 and analysis of its physicochemical characterization.

Soft rot is one of the top ten most dangerous plant pathogens in agricultural production, storage, and transport, and the use of microorganisms and their metabolites to control soft rot is a current research hotspot. In this study, we identified the antimicrobial substance in the metabolite of Paenibacillus polymyxa KH-19, and determined that the antimicrobial substance of this strain was an active protein. The protein was completely precipitated at 40-60% ammonium sulphate saturation and showed good inhibitory effects against seven pathogenic bacteria including Pectobacterium carotovorum BC2 and seven pathogenic fungi including Pyricularia oryzae. The MIC of the protein was 51.563µg/mL, temperature acid-base UV and light stability insensitive to protease, with high-temperature resistance. The antimicrobial protein was isolated and purified by DEAE-anion exchange column and Sephadex G-75 gel filtration chromatography, and the LC-MS/MS assay identified the protein as lysophosphatidyl esterase with a molecular weight of 25.255kDa. The purified antimicrobial protein increased the inhibitory effect against P. carotovorum BC2, with the diameter of the circle of inhibition being 26.50 ± 0.915mm. Bioinformatics analysis showed that the protein has the molecular formula of C1117H1732N316O338S5, encodes 224 amino acids, has an aliphatic index of 88.39, and belongs to the category of hydrophilic unstable proteins. The present study is the first report of an active protein with extreme thermoplastic and resistance to P. carotovorum BC2, which provides a reference for the preparation and application of the antimicrobial substances of P. polymyxa KH-19, as well as a theoretical basis for the study of the function of lysophosphodiesterase protein and its use as a microbial preparation.

Testing microbial pest control products in bees, a comparative study on different bee species and their interaction with two representative microorganisms

BackgroundThe evaluation of the impact of pesticides on non-target species, like bees, is a crucial factor in registration procedures. Therefore, standardized test procedures have been developed on OECD level assessing the effects of chemicals on honey bees or bumble bees. Unfortunately, these protocols cannot directly be adapted for testing products that contain microorganisms. Interest in the use of microorganisms has increased in recent years due to their specificity to target species while not harming non-target organisms. This study aimed to evaluate optimal conditions to assess the effects of microbial plant protection products on bee species according to currently available test protocols. Some of the most commonly used microorganisms for plant protection, Bacillus thuringiensis subspecies aizawai (B. t. a. ABTS 1857) and Beauveria bassiana (B. b. ATCC 74040) were tested on Apis mellifera, Bombus terrestris, and Osmia bicornis at different temperatures (18, 26, 33 °C) under laboratory conditions.ResultsExposure to the product containing B. t. a. ABTS 1857 resulted in higher mortality compared to B. b. ATCC 74040 in all tested bee species. A temperature-dependent effect towards higher mortality at higher temperatures of 26 °C or 33 °C was observed in O. bicornis exposed to both microorganisms. A. mellifera showed variable responses, but for B. terrestris there was mostly no effect of temperature when exposed to microorganisms in high concentrations. However, temperature affected longevity of bee species in the non-exposed control group. A. mellifera mortality increased with decreasing temperatures, while B. terrestris and O. bicornis mortality increased with increasing temperatures. A test duration of 15 or 20 days was found to be suitable for testing these microorganisms.ConclusionIn conclusion, 26 °C should be considered the worst-case scenario for testing B. bassiana on all tested bee species. For testing B. thuringiensis, a temperature of 33 °C is recommended for A. mellifera, whereas B. terrestris and O. bicornis should be tested at 26 °C.

Screening nitrogen-fixing cyanobacteria that can alleviate cadmium toxicity to rice and reduce cadmium accumulation in brown rice

Screening nitrogen-fixing cyanobacteria that can alleviate cadmium toxicity to rice and reduce cadmium accumulation in brown rice

Carbon dioxide capture, sequestration, and utilization models for carbon management and transformation.

The elevated level of carbon dioxide in the atmosphere has become a pressing concern for environmental health due to its contribution to climate change and global warming. Simultaneously, the energy crisis is a significant issue for both developed and developing nations. In response to these challenges, carbon capture, sequestration, and utilization (CCSU) have emerged as promising solutions within the carbon-neutral bioenergy sector. Numerous technologies are available for CCSU including physical, chemical, and biological routes. The aim of this study is to explore the potential of CCSU technologies, specifically focusing on the use of microorganisms based on their well-established metabolic part. By investigating these biological pathways, we aim to develop sustainable strategies for climate management and biofuel production. One of the key novelties of this study lies in the utilization of microorganisms for CO2 fixation and conversion, offering a renewable and efficient method for addressing carbon emissions. Algae, with its high growth rate and lipid contents, exhibits CO2 fixation capabilities during photosynthesis. Similarly, methanogens have shown efficiency in converting CO2 to methane by methanogenesis, offering a viable pathway for carbon sequestration and energy production. In conclusion, our study highlights the importance of exploring biological pathways, which significantly reduce carbon emissions and move towards a more environmentally friendly future. The output of this review highlights the significant potential of CCSU models for future sustainability. Furthermore, this review has been intensified in the current agenda for reduction of CO2 at considerable extends with biofuel upgrading by the microbial-shift reaction.

The causal association between gut microbiota and postpartum depression: a two-sample Mendelian randomization study.

An escalating body of clinical trials and observational studies hints at a plausible link between gut flora and postpartum depression (PPD). The definitive causal dynamics between these two entities remain shrouded in ambiguity. Therefore, in this study, we employed the two-sample Mendelian randomization approach to ascertain the causal link between gut microbiota and PPD. Summary-level GWAS data related to the human gut microbiota were obtained from the international consortium MiBioGen and the Dutch Microbiome Project (species). For PPD, GWAS data were derived from the FinnGen biobank, consisting 57,604 cases and 596,601 controls. The inverse variance weighted method (IVW) as the cornerstone of our analytical approach. Subsequent to this, a comprehensive suite of tests for pleiotropy and heterogeneity were conducted to ensure the reliability and robustness of our findings. We identified 12 bacterial taxa associated with the risk of PPD. Veillonellaceae, Ruminococcaceae UCG 011, Bifidobacterium adolescentis, Paraprevotella clara, Clostridium leptum, Eubacterium siraeum, Coprococcus catus exhibited an inversely associated with the risk of PPD. Alphaproteobacteria, Roseburia, FamilyXIIIAD3011group, Alistipes onderdonkii, Bilophila wadsworthia showed a positive correlation with the risk of PPD. The GWAS data derived from the MiBioGen consortium, DMP, and FinnGen consortium, may introduce selection bias. Moreover, the data primarily originates from European populations, hence extrapolating these results to diverse populations should be approached with caution. The etiological factors behind PPD remain enigmatic, alluding to the existence of potential undisclosed confounders. Based on this MR analysis, we found a causal relationship between certain gut microbial communities and PPD. Future clinical studies can further explore the treatment of PPD through the combined use of microorganisms. This not only offers insights into the pathogenesis of PPD but also lays the foundation for utilizing gut microbiota as biotherapeutics in treating neurological disorders.