Plasma medicine is an emerging field of research that aims to increase under - standing and utilization of the interaction of plasmas with living tissues and cells. Low-tem- perature atmospheric-pressure plasma is a multi-component system that includes such biologi- cally active agents as charged particles, reactive nitrogen and oxygen species, metastable-state molecules or atoms, and UV radiation. The main objective of this study was to investigate the effects on human blood cultures of exposure to the plasma components for different time periods (20 s, 40 s, 60 s, and 80 s). The present study recorded the scoring of micronuclei in both mono- and bi-nucleated lymphocytes as well as the apoptosis and necrosis of cells for each time period of exposure. Three blood samples for each experimental dose were compared with a non-exposed (control) group. In addition, the levels of interleukin-1β (IL-1β) and tumor necrosis factor alpha (TNF-α) were analyzed for each experimental dose and for the control samples. The results showed that the exposure of blood samples to the plasma jet yielded significant incremental differences in micronuclei, IL-1β, and TNF-α, except in the first dos- age group. Based on these results, we conclude that plasma can be used to repair tissues, cure diseases, and treat tumors.