Microbial growth and contamination in aviation fuel storage tanks and aircraft wing tanks cause metal corrosion, plugging of the fuel filter, and increased maintenance costs associated with these problems. This paper reports the microbiologically induced corrosion (MIC) and electrochemical behavior of aluminum alloy (AA 2024) in the presence of hydrocarbon-degrading bacteria Bacillus cereus ACE4 (a Gram-positive bacteria) and Serratia marcescens ACE2 (a Gram-negative bacteria). Electrochemical impedance spectroscopy and metallographic analysis of the metal AA 2024 exposed to a simulated fuel tank environment showed that the bacteria caused pitting corrosion. Scanning electron microscopy−energy-dispersive X-ray spectroscopy analysis (SEM−EDAX), atomic force microscopy (AFM), and Fourier-transform infrared spectroscopy (FTIR) analyses of the aluminum alloy coupons with bacterial biofilm developed after exposure to minimal salt medium confirmed that extracellular polymeric substances accumulate with exposure...