Microbiological Quality Indicators Research Articles

Effect of the shifting from multi-layer systems towards recyclable mono-material packaging solutions on the shelf-life of portioned semi-hard cheese

Packaging with recyclable plastic films could be an interesting alternative solution to extend shelf-life of portioned cheese. Two multi-layer materials of different types of one polymer (MonoMAP1 and MonoMAP2) with different barrier properties (O2: 13 and 20 cm3(STP)/(m2 × 24 h), 0 % RH; CO2: 65 and 92 cm3(STP)/(m2 × 24 h), 0 % RH) and coupled with modified atmosphere (MAP) were considered. Their performances in affecting cheese stability were compared to those of conventional multi-layer solutions under-vacuum (MultiVAC) and MAP (MultiMAP). Microbiological and physicochemical quality indicators were considered for a shelf-life study. In MonoMAP2, the initial loss of carbon dioxide and concomitant permeation of oxygen into the pack caused an earlier proliferation of molds with values of 4.7 log cfu g−1, leading to its inapplicability. In the other packaged samples, the development of rancid volatile compounds resulted the more sensitive quality indicator to be used for shelf-life assessment, with a critical threshold perceived by panelists around 520 mg/kg. Cheese packaged in MonoMAP1 showed a shelf-life associated to rancidity development of about one third lower than that of MultiVAC and MultiMAP ones.

ФОРМИРОВАНИЕ КАЧЕСТВЕННЫХ ХАРАКТЕРИСТИК ПИВНЫХ НАПИТКОВ С ПЛОДОВО-ЯГОДНЫМ СЫРЬЕМ

Beer drinks are gaining more and more popularity, they are distinguished by original taste and aroma characteristics. Consumer interest in beer drinks with the use of fruit and berry raw materials is noted. The objective of the study is to develop a technology for beer drinks using fruit and berry raw materials and to form their quality characteristics. Tasks: to determine the dosage of juices (cherry, currant, raspberry) in the recipe for a beer drink based on light unfiltered beer Prazhskoye by studying the standardized quality indicators and identifying the best sample by overall characteristics; establishing the shelf life of beer drinks with the addition of juices by analyzing safety indicators in accordance with TR CU 021/2011; ma-king adjustments to the technological process to extend the shelf life of beer drinks. The objects of the study were beer drinks based on light unfiltered beer Prazhskoye with the addition of clarified cherry, currant and raspberry juice, produced according to GOST 32102-2013 Group H54 "Canned goods. Juice products. Concentrated fruit juices. General specifications" in quantities of 10, 20, 30 % of beer wort. In the work, generally accepted methods of analysis of organoleptic, physicochemical and microbiological quality indicators were used. The technology of beer drinks was developed, the quantitative dosage of juices was selected: cherry – 20 %, currant – 10, raspberry – 30 % of beer wort. A comprehensive assessment of the quality of the developed drinks was carried out according to organoleptic, physicochemical, microbiological quality indicators. The shelf life of beer drinks was determined – 30 days. The obtained results allow the enterprise to expand the range of beer products and satisfy consumer demand for high-quality domestic drinks with fruit and berry raw materials.

Water Quality Assessment in an Area Degraded by Urban Solid Waste in Southern Brazil

ABSTRACTIn most Brazilian municipalities, urban solid waste (USW) is disposed of in open dumps, with numerous sites now being closed, monitored, and rehabilitated. One location, the “old dump” area in the Portão municipality (southern Brazil), previously served as an irregular repository for USW and industrial solid waste (ISW). Its use continued until 1993 when it was closed for a natural remediation process. For this reason, this study aimed to assess groundwater quality in the Portão “old dump,” employing physicochemical, microbiological, and toxicity quality indicators. The study's scope was extended to encompass the Portão Stream as well. Notably, the second collection campaign revealed elevated dissolved aluminum (Al3+), iron (Fe2+), and nitrate levels in all groundwater samples (from PZi wells, i = 1–6), with the PZ3 monitoring well experiencing the most pronounced impact. Further quality indicators, such as total phosphorus, thermotolerant coliforms, BOD5, as well as Al3+ and copper (Cu2+) levels in the second water collection campaign from Portão Stream, were classified as Class 4, suggesting that the water is suitable only for navigation and aesthetic purposes. Conductivity analysis identified Portão Stream as another impacted area. Moreover, during the second collection campaign, the study uncovered an escalation in micronuclei (MN) frequency and chromosomal alterations in PZ5. Downstream water samples from Portão Stream exhibited similarly heightened MN levels. This study's investigation into water quality is a concrete example of the implications of improper USW management. These findings underscore the pressing need for effective waste management strategies to mitigate environmental degradation and protect public health.

Possibilities of an Electronic Nose on Piezoelectric Sensors with Polycomposite Coatings to Investigate the Microbiological Indicators of Milk.

Milk and dairy products are included in the list of the Food Security Doctrine and are of paramount importance in the diet of the human population. At the same time, the presence of many macro- and microcomponents in milk, as available sources of carbon and energy, as well as the high activity of water, cause the rapid development of native and pathogen microorganisms in it. The goal of the work was to assess the possibility of using an array of gas chemical sensors based on piezoquartz microbalances with polycomposite coatings to assess the microbiological indicators of milk quality and to compare the microflora of milk samples. Piezosensors with polycomposite coatings with high sensitivity to volatile compounds were obtained. The gas phase of raw milk was analyzed using the sensors; in parallel, the physicochemical and microbiological parameters were determined for these samples, and species identification of the microorganisms was carried out for the isolated microorganisms in milk. The most informative output data of the sensor array for the assessment of microbiological indicators were established. Regression models were constructed to predict the quantity of microorganisms in milk samples based on the informative sensors' data with an error of no more than 17%. The limit of determination of QMAFAnM in milk was 243 ± 174 CFU/cm3. Ways to improve the accuracy and specificity of the determination of microorganisms in milk samples were proposed.

Influence of structure-forming recipe ingredients on the quality of pumpkin marmalade

Analyzing the assortment of the market for marmalade products, it is worth noting that there is a tendency to introduce natural products in the form of vegetable or fruit puree into the recipe composition, as the demand for confectionery products for therapeutic, prophylactic, and functional purposes, as well as for products with dietary properties, is growing. This work aims to study the influence of structure-forming recipe ingredients on the quality of pumpkin jelly marmalade. Pumpkin puree was used as the primary raw material for producing jelly marmalade, food agar was used as a gelling agent, and natural honey was used as a sweetener. During the study, organoleptic, physicochemical, and microbiological quality indicators of semi-finished pumpkin products and finished marmalade samples were determined. The color assessment of the finished products was carried out using a colorimeter NR-UO (China). Pumpkin semi-finished and finished products meet regulatory documentation requirements regarding organoleptic, physico-chemical, and microbiological quality indicators. When food agar is used as a gelling agent, the degree of color increases due to the transparency of the structure, and the red (+a*) and yellow (+b*) components in samples containing food agar are successfully combined and give the finished product an attractive orange color. To increase the nutritional -value, it is proposed to use cinnamon or coconut flakes, which have beneficial properties, to sprinkle the surface of finished products. The price for 1 kg of pumpkin jelly marmalade was: control sample - 335.46 rubles, “Məkhəbbət” with cinnamon - 748.85 rubles, “Məkhəbbət” with coconut flakes - 725.36 rubles. For a new type of confectionery products, regulatory and technical documentation STO, TI, RC 00493497-005-2022 “Halal” pumpkin jelly marmalade “Məkhəbbət” has been drawn up.

Confectionery products stored in permafrost conditions

Industrial development of the Arctic territories is a crucial direction in the development of our country. Expanding the market for confectionery products requires increasing their safety in various climatic conditions, which is only possible by ensuring quality indicators that guarantee their specified shelf life. Studies of confectionery products stored in conditions of unregulated negative temperatures of permafrost, according to organoleptic, physico-chemical and microbiological quality indicators, have been conducted. “Iris Kiss-Kiss” candies, “Strawberry” cookies, homemade crackers, biscuits, and chocolate were stored at unregulated low temperatures. In cookies, crackers, and biscuits, a foreign odor and rancid taste caused by oxidation products of the fat fraction were detected. As for chocolate after storage, heavy bloom and significant changes in structure and consistency were revealed. The moisture content of the chocolate increased. Storage conditions at low temperatures and high humidity increase the risk of microorganism development and microbiological spoilage. The results obtained allow concluding that long-term storage of confectionery products is possible under unregulated low temperatures.

Development of beer drinks recipe and technology with fruit and berry raw materials

This study aimed to develop recipes and technology for beer drinks based on fruit and berry raw materials to broaden the variety of domestic drinks and achieve high-quality, safe products with good flavor characteristics. Beer drinks were the objects of the study. The main ingredients for the beer drink were unfiltered light beer "Prazhskoye" from a private brewery in Kemerovo, as well as cherry, raspberry, and blackcurrant juice. The study used generally accepted methods of analysis of organoleptic, physicochemical, and microbiological quality indicators. Recipes and technology of beer drinks were developed. The dosages of cherry, raspberry, and currant juices were determined during the study of the influence of the introduced juices, the complex estimation of qualitative indicators of beer drinks was performed, and the shelf life was determined. The obtained results enable the company to expand its beer product line and meet customer demand for high-quality domestic beverages made from fruit and berries.

Parametric statistical analysis of microbiological water quality indicators

During the summer months from 2014 to 2016, water was sampled at two lakes in Zagreb, Jarun and Bundek, with the aim of determining the number of colonies of microbiological indicators of Escherichiae coli and intestinal enterococci. We have investigated the dependence of microbiological water quality indicators on sampling points. Also, we were interested in determining the influence of the season of the year. This research identified that regular monitoring of microbiological indicators is necessary, especially at locations used by people for recreation or other purposes, in order to prevent possible infections.

High-Pressure Processing for Cold Brew Coffee: Safety and Quality Assessment under Refrigerated and Ambient Storage.

Cold brew coffee (CBC) has gained in popularity due to its distinct sensory experience. However, CBC can pose a risk for bacterial pathogens if not stored properly. High-Pressure Processing (HPP) is a nonthermal technology that can improve the safety of CBC while maintaining its quality. In this study, CBC made from ground roasted coffee grains was processed at 600 MPa for 3 min and stored at 4 or 23 °C for 90 days. The microbiological quality indicators remained stable throughout the study period. Physicochemical and quality parameters, such as pH, total dissolved solids, titratable acidity, color, total phenolic compounds and antioxidant activity, were not significantly affected by HPP. Both unprocessed and HPP CBC samples showed changes in pH, titratable acidity and color stability after 60 days at 23 °C. Unprocessed CBC samples spiked with Escherichia coli O157:H7, Listeria monocytogenes and Salmonella enterica showed decreased counts, but the pathogens were still detectable after 60 days at 4 °C and after 90 days at 23 °C. HPP achieved a >6-log10 reduction in the species tested, with non-detectable levels for at least 90 days at both storage temperatures. These findings suggest that HPP can effectively control vegetative pathogens and spoilage microorganisms in CBC while preserving its quality attributes.

Microbiological indicators of quality and safety of hard rennet cheese with linseed content during storage

Food fortification is one of the most important processes for improving the nutritional quality and quantity of food products. Currently, the development of new recipes for dairy products is one of the driving forces of the dairy industry. The purpose of this study was to determine the suitability of the developed hard cheese with flax seeds for storage under the conditions defined by the standard. Microbiological studies were performed according to standard methods, which included the preparation of samples for the study, carrying out tenfold dilutions and sowing them on selective and storage media. Coliform bacteria and lactic acid microorganisms were determined according to DSTU 7357:2013, Salmonella and Listeria bacteria according to DSTU EN 12824:2004 and DSTU ISO 11290-1:2003, respectively. The developed Gouda-type hard cheese with flax seeds showed high microbiological indicators, which characterize its safety and quality, during storage at the temperature regime t... +5 ± 1 °C for 45 days. In particular, according to the amount of coliform bacteria, the product during 45 days of storage had a titer one order of magnitude higher than the maximum allowable limit (0.01 g), and according to the content of Staphylococcus aureus, it had an amount almost 20 times lower than the maximum allowable standard. Pathogenic microorganisms such as L. monocytogenes and Salmonella spp. were not detected in 25 g of the product during the entire storage period. At the end of the storage period, the number of lactic acid bacteria in the cheese was 8.0 ± 0.1 × 108 CFU/g. Therefore, the hard rennet cheese with flax seeds developed by us should show functional properties, both due to the content of flax seeds – a source of omega-3 fatty acids, and due to the vital activity of lactic acid microflora.

Assessing differential land use impacts on soil quality: A method based on log-response ratios and polygonal projections

Soil quality indices (SQI) used for assessing soil degradation are often developed using additive scoring functions. However, these SQI may lack reference values for interpreting their outputs and the capacity to differentiate changes in specific variables. To overcome these limitations, this study introduces SQI using Log Response Ratios (LRR) as measures of size effects caused by land use in physical, chemical, and microbiological soil quality indicators. LRR vectors projected 2D polygons with condensed change measures along their perimeters. This method was tested in andosols within the southeastern region of Antioquia, Colombia. These soils were subjected to contrasting stages of degradation determined by the extent of A-horizon removal due to land use practices. This study shows that mining and agriculture have detrimental effects on soil organic carbon and water contents, and that size effects vary significantly between land uses (p < 0.05). Microbiological features also exhibit distinct size effects, such as populations of culturable mesophilic bacteria and fungi, microbial basal respiration, spore density of arbuscular mycorrhizal fungi (AMF), their diversity, and total glomalin-related soil proteins (p < 0.05). The SQI proposed exhibited a negative correlation with SQI computed from scoring additive functions either considering the entire dataset (R2 = 0.87) or a minimum dataset (R2 = 0.90). This approach underscores the utility of using LRR geometrical analysis to assess global soil quality differences among land uses (p < 0.01), offering a visual, quantifiable representation of the effects of each land use over specific soil quality indicators.

Microbiological indicators of the quality of cyprinid fish of the Volga-Caspian region

The intensive development of industrial and amateur fishing, due to the expansion of the range of fish products, as well as the problem of ensuring the safety of fish raw materials, determined the relevance of studying the quality of aquaticbiological resources, including microbiological criteria. Samples of muscle tissue of bream, roach, rudd, gaster and carp caught in the river zone in the periods 2010–2014 and 2020–2022, as well as bream and roach in the marine zone in similar periods, served as the material for determining microbiological quality indicators. The quality assessment of the examined fish according to microbiological criteria was carried out in accordance with regulatory documents. In the lower reaches of the Volga River, bacterial contamination of muscle tissue of freshly caught mature carp fish varied within 0.07±0.01–2.42±0.40 thousand. CFU/g. The number of bacteria in the muscles of frozen fish varied from 0.24±0.05–9.34± 0.20 thousand. CFU/g. In the northern part of the Caspian Sea, bacterial contamination of the muscle tissue of freshly caught cyprinid fish varied in the range of 0.14±0.02–4.08±0.84 thousand. CFU/g. The number of bacteria in the muscles of frozen fish varied from 0.13±0.03–5.13±0.65 thousand. CFU/g. Indicator microorganisms (BGCP, staphylococci, salmonella, listeria and parahemolytic vibrions) were absent in the maximum permissible mass of muscle tissue, which, against the background of a low total number of microorganisms, confirmed the satisfactory quality of mature individuals of the surveyed commercial fish species. These species could be used for food without restrictions, with appropriate processing in compliance with the necessary rules and regulations.

Municipal separate storm sewer system (MS4) dry weather flows and potential flow sources as assessed by conventional and advanced bacterial analyses

Municipal separate storm sewer systems (MS4s) function in urbanized areas to convey flows during both wet weather (i.e., stormwater) and dry weather (i.e., urban runoff as well as subsurface sources of flow) to receiving waters. While urban stormwater is known to contain microbial and chemical pollutants, MS4 dry weather flows, or non-stormwater discharges (NSWDs), are much less studied, although they are also known to contain pollutants, especially when these flows include raw sewage. In addition, some natural NSWDs (e.g., from groundwater infiltrating MS4 pipes) are critical for aquatic habitat protection. Thus, it is important to distinguish NSWD sources to prevent non-natural flows while retaining natural waters (i.e., groundwater). Here, MS4 dry weather flows were assessed by analyzing water samples from MS4 outfalls across multiple watersheds and water provider service areas in south Orange County, CA; potential NSWD sources including sewage, recycled water, potable water, and groundwater were sampled and analyzed for their likely contributions to overall NSWDs. Geochemical and microbiological water quality indicators, as well as bacterial communities, differed across NSWDs, yet water quality within most locations did not vary significantly diurnally or by sampling date. Meanwhile, NSWD source waters had distinctly different bacterial taxa abundances and specific bacterial genera. Shared geochemical and microbial characteristics of certain sources and outfall flows suggested the contributions of sources to outfall flows. The average proportions by sources contributing to MS4 outfalls were further estimated by SourceTracker and FEAST, respectively. The results of this study highlight the use of multiple tools when assessing chemical and microbiological water quality to predict sources of NSWDs contributing to urban MS4 flows during dry weather. This information can be used to support management actions to reduce unnatural and high risk sources of dry weather drainage while preserving natural sources important to environmental health in downstream receiving waters.

LACTOSE-FREE ICE CREAM TECHNOLOGY BASED ON SECONDARY DAIRY RAW MATERIALS

Expanding the assortment of lactose-free dairy products, namely, ice cream for people who cannot tolerate lactose is a promising direction of research. The purpose of the work is to develop the technology of milk lactose-free synbiotic yogurt ice cream based on buttermilk protein-lipid concentrate, obtained by ultrafiltration/diafiltration, with the addition of natural flavoring and food additives, and the study of its quality indicators. A protein-lipid concentrate of buttermilk with a low lactose content was obtained by ultrafiltration/diafiltration, and its quality indicators were determined. The concentrate has a high protein content (16.01%) and a low lactose content (1.28%). Protein-lipid concentrate fermented with synbiotic sourdough is the main component included in the formulation of lactose-free synbiotic yogurt ice cream. The main physico-chemical parameters of the obtained fermented protein-lipid concentrate were determined, which ensures a rational content of the main components of the chemical composition in the finished lactose-free ice cream (it does not contain lactose, the protein content is 8.46%) and a high content of useful microflora (bifido- and lactobacilli). A mixture for lactose-free synbiotic yogurt ice cream was obtained according to a scientifically based recipe. A sample of lactose-free ice cream was made from the mixture using traditional technology, and organoleptic, physico-chemical and microbiological quality indicators were investigated. A comparison of the quality indicators of the studied sample with the control, which was taken as classic yogurt ice cream, was carried out. The mass fraction of lactose in samples of lactose-free ice cream is 0,011%. The developed lactose-free synbiotic yogurt ice cream has high probiotic activity (contains lacto- (3.0•108 CFU/g) and bifidobacteria (3.5•109 CFU/g)).

The level of aquatic yeast as a possible indicator of microbiological quality of urban recreational water reservoirs

ABSTRACT Sanitary indicators standardly used in water quality monitoring refer to fecal microorganisms or cyanobacterial toxins, but at present they do not include fungi, despite the fact that many fungi can pose potential health hazards. The aim of study was a quantitative and qualitative assessment of yeast in waters of urban artificial recreational reservoirs in relation to microbiological and sanitary cleanliness indicators and to demonstrate that yeast could be an indicator of anthropogenic pollution. In total, 18 species of yeast were isolated, including 13 classified as clinically relevant. Tested reservoirs exceeded the standards of sanitary indicators acceptable for bathing water. Since isolated yeasts are associated with living organisms, their presence in the water of urban recreational reservoirs may be a public health concern. Therefore, a mycological analysis should be incorporated into standard water quality assessment procedures. The results obtained are very promising, but further research is still needed.

Assessment of organoleptic, microbiological and physico-chemical indicators of feed quality

Microbiological, organoleptic and physico-chemical indicators of the quality of feed selected in the Kaluga region are presented. The conditions of preparation of the studied samples are optimized. To assess the required indicators, GOST methods and traditional methods based on the use of chemical and biological methods, as well as organoleptic analysis, were used.

Application of textured wheat flour in bakery technology

The paper presents the study results on the effect of textured wheat flour on organoleptic, physical and chemical, and microbiological quality indicators, and storage stability of bakery products. The study objects were samples of bakery products made by the sponge and dough method with and without prior dough fermentation and those made by the quick sponge method. The addition of the studied flour in the amount of 40% by flour weight did not significantly affect the organoleptic quality indicators, as well as the moisture and acidity of bakery products made by different methods. The protein content in the test samples was 7.0–7.5 g per 100 g of the product. The lowest degree of staleness 72 h after baking could be observed in bakery products made by the sponge and dough method with prior dough fermentation. Products made by the sponge and dough method with and without prior dough fermentation showed the first signs of mold on storage day 15. The first signs of rope spoilage were found after 144 h of storage under provoking conditions in bakery products made by the sponge and dough method with prior dough fermentation. The study results yielded bakery products made from wheat flour and supplemented with textured wheat flour in the amount of 40% by flour weight. In accordance with TR TS 022/2011 ‘Food products in terms of their labeling’, bakery products supplemented with 40% textured wheat flour made by the sponge and dough method with and without prior dough fermentation are regarded as a source of dietary fiber. A draft technical documentation has been developed for bakery products made from wheat flour supplemented with textured wheat flour.

Microbiological indicators as sensitive indicators in the assessment of areas contaminated by heavy metals

Context As one of the world’s largest mining spills, the Aznalcóllar pyrite mine accident in Spain in 1998 resulted in ~45 km2 of agricultural soils polluted by arsenic and heavy metals. The Guadiamar Green Corridor (GGC) helped with soil remediation but residual pollution is still detected 20 years later. Aims Several methodologies based on chemical indicators have been used to assess the recovery of these areas. However, simple microbiological indicators (e.g. microbial activity and biomass, and metabolic quotient (qCO2)) are yet to be uesed. The aim of this study was to evaluate areas contaminated by arsenic and heavy metals using microbiological indicators of soil quality. Methods We used a systematic random sampling design to collect soil samples from two soil groups with different recovery trajectories. We analysed the total and water-soluble concentrations of arsenic (As), lead (Pb), copper (Cu), and zinc (Zn), the main soil properties, and bioassays including microbial activity and biomass, and metabolic quotient or microbial stress (qCO2). Key results Twenty years after the accident, soils with As and Pb concentrations that consistently exceeded regulatory levels had altered soil microbial biomass and functioning. Although overall rates of microbial respiration were not significantly different between polluted soils, microbial biomass was lower and qCO2 was higher in the more polluted than in less polluted soils. Conclusions The metabolic quotient and microbial biomass are sensitive indicators in the monitoring over time of soil polluted by arsenic and heavy metals. Implications Microbial indicators must be considered in the assessment of potential ecotoxicity and in the evaluation of soil biological properties influencing soil recovery in the long term.

Biopharmaceutical and microbiological research of fennel fruit infusions

Aim. To develop and study physico-chemical, pharmaco-technological, pharmacognostic and microbiological quality indicators of infusions obtained by extraction of the plant raw material of fennel fruits of different degrees of grinding and from different types of packaging. Materials and methods. The study objects were model samples of fennel fruits of different degrees of grinding and water extracts obtained on their basis and from different types of packaging. Water extracts were prepared according to the SPhU method and the patient information leaflet on fennel fruits. The study of pH of solutions, extractives, identification of the main biologically active substances (BAS), and microbiological purity of water extracts were performed by the SPhU methods; the quantitative determination of the essential oil was conducted by direct alkalimetric titration with 0.01 mol/l NaOH solution in the presence of methylene blue and phenolphthalein indicators according to the Guidelines of the Ministry of Health of Ukraine. Results and discussion. Extractives in model samples of infusions obtained from filter bags vary from 0.43 to 0.48 % without filter bags (samples 1, 3, 5, 7) – from 0.52 to 0.55 %, and its amount is the lowest in sample 9 – 0.37 %. However, the essential oil content is the highest in sample 9, these are whole fennel fruits from the pack (0.15 %); slightly higher indicators are in model samples without filter bags (samples 2, 4, 6, 8). The pH value is slightly higher in infusions obtained from filter bags than without them (in the range of 6.61-6.70 and 6.22-6.25, respectively). However, the infusion obtained from a nylon pyramid bag (sample 7) had a pH value close to infusions obtained from the fennel medicinal raw material without packaging (6.25). The presence of infusions of anethole and terpenoids in model samples has been proven by thin layer chromatography. The microbiological studies conducted have shown that the use of the method of making water extracts from the patient information leaflet allows obtaining conditionally sterile infusions. Conclusions. The indicators studied (transparency, color, odor, taste, extractives, identification and quantitative determination of BAS) can be used in the analysis of water extracts from the medicinal plant raw material produced in filter bags.

Specialized product in the form of a biotechnological complex of bacterial metafiltrates for selective sorption of endotoxins and metabolic correction of intestinal microflora

The intestinal microflora of the human body performs vital regulatory functions. In the course of the study, a biotechnological complex has been developed in the form of dietary supplements, the functional properties of which are aimed at regulating the intestinal biocenosis, inhibiting pathogenic and conditionally pathogenic microflora, as well as developing a useful part of the microbiota. The qualitative and quantitative composition of the specialized product includes, mg/5.5 g, floration (soluble dietary fiber) - 1,375 (1,238); metafiltrates Bifidobacterium bifidum - 620, Bifidobacterium breve - 600, Lactobacillus delbrueckii - 500, Hericium coralloides - 242.5, Propionibacterium freudenreichii - 100; lactulose (50 %) - 400; postbiotic metafiltrate Subtizym - 200; silicon dioxide - 30; endofulvin (Endofulvin, humic acids) - 9.62 (7.5); chitosan - 5; para-aminobenzoic acid - 2; nicotinamide (vitamin B) - 1.81 (1.8); cysteine - 1.25; calcium pantothenate (vitamin B) - 0.654 (0.6); riboflavin (vitamin B) - 0.16. An innovative feature of the production technology is a liquid concentrated form of dietary supplements, which ensures rapid passage through the stomach, the preservation of active principles and the effectiveness of the implementation of specified functional properties. The organoleptic, microbiological and physicochemical indicators of quality and safety have been studied, the results of which make it possible to determine the regulated characteristics and shelf life - 1 year at the temperature of 0-25 °C, relative humidity of not more than 60 %. The developed complex is recommended for use in the amount of 5.5 g of concentrate 3 times a day; course duration - 1 month. The product has passed production tests at ArtLife LLC (Tomsk).