Microbiological Counts Research Articles

Influence of aerial and vacuum packaging on the quality and shelf life of beef treated with extra virgin olive oil

The aim of this study was to evaluate the effects of olive oil as a natural preservative and aerial and vacuum packaging on the quality and shelf life of raw beef. Meat samples were divided into four treatments i.e. T0= with 1% olive oil and aerial packaged, T1= without olive oil and aerial packaged, T2 = with 1% olive oil and vacuum packaged and T3 = without olive oil and vacuum packaged. All samples were kept at refrigerated temperature. Days of interval were 0, 5, 10 and 15. The samples were tested for sensory properties (color, flavor, tenderness, juiciness and overall acceptability), physicochemical characteristics (water holding capacity, cooking loss, pH); biochemical properties (TBARS), and microbiological counts (TVC, TCC and TYMC). During the whole storage process, the sensory and instrumental color value were considerably higher (P<0.05) in T0 and T2 treatments. WHC in treatment T2 was higher, CL was lower in T0 treatment and pH in treatment of T0, T1 were considerably higher (P<0.05) than in the T2 and T3 group. The values of TBARS, viable count, coliform count, and yeast-mold count were significantly lower in T0 and T2 group. Based on the findings of this study, it is possible to conclude that olive oil treated beef in aerial and vacuum packaging storage technique can be used in the future for meat preservation, providing antioxidant and antimicrobial agents as a value addition by inhibiting lipid oxidation and extending the shelf life of beef.

The impact of storage temperature and packaging material on the quality of ultrasound treated sweet lime juice

The aim of this study is to examine the shelf life of ultrasound (US) treated sweet lime juice at 4, 15, 25, 30, and 35 °C and in two different packaging materials (coextruded ethylene vinyl alcohol copolymer [EVOH] and multi-layered polyethylene terephthalate [ML-PET] films). The US-treated sweet lime juice (0.7 W/cm3) was analyzed for the microbial count, enzyme activity, bioactive compounds degradation, and sensory attribute for a shelf-life of 37 days. Different packaging materials and storage temperatures had a significant effect (p < 0.05) on the microbial, enzyme inactivation, and biochemical attributes but had no significant influence (p > 0.05) on pH, acidity, and soluble solids in the samples. On a zeroth day, the US-treated sample showed an increase in bioactive compounds and vitamin C due to the increased cavitation effect. US-treated juice (0.7 W/cm3) retained 88.8% vitamin C and 100% antioxidants after 37 days of storage at 4 °C. Untreated and US-treated juice (0.7 W/cm3 at 0 day) got sensory scores of 8.1 and 7.5 out of 9 respectively. The juice stored in ML-PET had a better shelf life and nutrient retention than in the EVOH pouch. Interestingly, the sensory and vitamin C profile was above the allowable limit even after 37 days of storage. However, the microbiological count (>6 log cfu/mL) limited shelf life up to 37, 17, 11, 5, and 3 days, at 4, 15, 25, 30, and 35 °C for the juice packed in ML-PET pouch.

Rice flour coating supplemented with rosemary essential oil to preserve the internal, microbiological, and sensory quality of quail eggs

AbstractCoatings of rice flour (RF), reinforced or not with rosemary essential oil (ROS), were used to evaluate changes in the internal quality of quail eggs stored at room temperature. Quality parameters [egg weight loss (EWL, %), Haugh unit (HU), yolk index (YI), albumen and yolk pH], microbiological (counts of total aerobic mesophilic bacteria and Enterobacteriaceae) and sensory (colour, aroma, odour, texture, taste, and general acceptability) parameters were evaluated during the experiment. Compared to the number of total aerobic mesophilic bacteria on the shell of uncoated eggs (2.02 ± 0.49; 1.78 ± 0.20 log10 CFU mL−1), RF/ROS exhibited significant inhibition effects for these bacteria on 0. (1.16 ± 0.25 log10 CFU mL−1) and 21. (0.84 ± 0.11 log10 CFU mL−1) days of storage at room temperature. On days 7, 14, and 21, RF had no effect on HU (P &gt; 0.05), but eggs coated with RF/ROS had significantly higher HU (P &lt; 0.05) than uncoated eggs from day 14, maintaining grade AA (73.88 ± 2.67) on day 21, while uncoated eggs had grade A (68.90 ± 1.55) at that time. The RF coating reinforced with ROS was a bioactive, efficient, and safe formulation for application based on internal quality, microbiological and sensorial aspects of quail eggs.

Adipose-derived Stem Cells Improving Inflammatory and Proliferative Responses in an Infected and Ischemic Ulcer Model in Type 1 Diabetic Rats: Insights Into Bacterial Count, Stereological Parameters, microRNA-21, and FGF2 Regulation

Background: Adipose-derived stem cells (ADSCs) have been shown to enhance wound healing in rats with type 1 diabetes (DM1). Objectives: This experimental study aimed to explore how ADSC administration affects bacterial count, wound size, biomechanical and stereological parameters, and the expression of microRNA-21 and FGF2 in a rat model of infected, ischemic, and delayed wound healing in DM1. Methods: Twenty-four male adult Wistar rats weighing less than 250 g were randomly assigned to four groups (n = 6 per group). Type 1 diabetes was induced in all animals, resulting in the development of a delayed, ischemic, and infected wound model. The CGday4 and CGday8 groups served as controls. In the AGday4 group, the animals received allograft h-ADSs and were euthanized on day four after surgery. Similarly, in the AGday8 group, the animals received h-ADSs and were euthanized on day eight after surgery. Microbial colony counts, wound size, stereological parameters, and the expression of microRNA-21 and FGF2 were evaluated in this study during the inflammation (day 4) and proliferation (day 8) stages of wound healing. Results: We demonstrated that h-ADSs significantly reduced microbiological counts compared to the control group on days 4 and 8. Moreover, in the AGday8 group compared to the AGday4 group, this decline in microbiological counts was even more pronounced. Moreover, we observed that the stereological characteristics in the AGday4 and AGday8 groups were significantly superior to those in the CG groups. Additionally, the AGday4 and AGday8 groups exhibited smaller ulcer area sizes compared to the CG groups. Furthermore, the AGday4 and AGday8 groups demonstrated higher expression levels of FGF2 and microRNA-21 than the CG groups on days 4 and 8. Notably, on day 8, the AGday8 group’s outcomes surpassed those of the AGday4 group (P &lt; 0.01). Conclusions: Through lowering microbial counts, modifying stereological parameters, microRNA-21, and FGF2 expression, the administration of hADS dramatically speeds up the healing of MARS-infected and ischemic ulcers in DM1 rats.

Assessment of Soil Properties Using Geospatial Techniques at DDUCE-OF Farm

The purpose of this study was to identify the geographic variability and mapping of different soil properties using geospatial techniques at DDUCE-OF Farm. For this purpose, surface soil samples (0-15 cm depth) were collected with the help of GPS at definite locations of research farm area at DDUCE-OF, CCSHAU, Hisar during rabi, 2021. These samples were analyzed in the laboratory for various physico-chemical and biological properties. Soil properties of DDUCE-OF farm revealed that soils are sandy loam to loam in texture having pH ranged from 7.15 to 8.65 and electrical conductivity (EC) ranged from 0.30 to 2.75 dS/m. The soil organic carbon (SOC) content was observed medium to high (0.41 to 0.94%).The available N was low (126 to 196 kg/ha), P ranged from 8.50 to 23.5 kg/ha (from low to high in content) and available K was found medium to high and ranged from 128.5 to 554.0 kg/ha. The available S ranged between 60 to 725 mg/kg and was observed sufficient. The soil microbiological bacterial count status ranged between 4.6x103-9.8x109 CFU count per g of soil in various blocks of organic farm. The DTPA-extractable micronutrients Zn (1.00-4.47 mg/kg), Fe (7.26-19.92 mg/kg), Mn (3.88-17.77 mg/kg) and Cu (0.93-4.64 mg/kg) were sufficient in amount and heavy metal contents (Ni, Pb, Cd, Cr, As) were found below their permissible limit. The study concluded that the soil mapping and survey is significant because it aids in the evaluation of soil qualities and their application in organic farming.

The leavening Ability of many lactic acid bacteria Isolated from Spontaneous Sourdough

Sourdough technology has been used to make bread for thousands of years. While simple, it involves complex interactions that determine microbial communities. However, evidence for lactic acid bacteria-yeast interactions in sourdough is not yet sufficiently available. The interaction between lactic acid bacteria and yeast has been examined. This study aimed to isolate lactic acid bacteria in wheat sourdough and determine their functional properties, such as acidification kinetics, microbiological counts, exopolysaccharide (EPS) production, and leavening ability. The characterization of the isolates revealed the presence of 32 different strains. In addition, six strains with the best performance were chosen to be used as starters for monoculture and co-culture with baker’s yeast. The lowest PH values and the highest total titratable acidity (TTA) values were observed with co-cultures. The growth rate analysis showed a negative interaction, whereas the lactic acid bacteria population was affected by yeast, while the yeast population size was rarely impacted, regardless of the strain. We found promising results for lactic acid bacteria strains regarding fermentation ability, which showed a higher ability to raise dough in monoculture. Two strains (2-1C2 and 13-1S2) showed interesting technological potential due to exopolysaccharide production and could be used to raise the dough without adding baker’s yeast.

Association between Mastitis Occurrence in Dairy Cows and Bedding Characteristics of Compost-Bedded Pack Barns.

Compost-bedded pack barns (CB) are receiving increasing attention as a housing system that can potentially improve the welfare of dairy cows. This study characterized the frequency and profile of pathogens isolated from clinical (CM) and subclinical (SCM) mastitis in dairy cows housed in CB. It evaluated the association between mastitis occurrence and bedding characteristics in CB systems. Over six months, seven dairy herds were visited monthly for milk and bedding sample collections. Milk samples from mastitis cases were submitted to microbiological identification by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF MS). Bedding samples were submitted to physical-chemical (pH, organic matter, moisture, and carbon to nitrogen ratio) and microbiological counting (total bacterial counts, coliforms, streptococci, and staphylococci) analyses. Regression analysis was used to determine the association between mastitis occurrence and CB characteristics. Our results showed that Escherichia coli and environmental streptococci were the most frequently isolated pathogens from CM cases, while Staphylococcus chromogenes and contagious pathogens (Staphylococcus aureus and Streptococcus agalactiae) were the most commonly isolated from SCM cases. Bedding moisture content was positively associated with the incidence of CM. The bedding carbon to nitrogen ratio was negatively associated with the incidence of SCM, and the bedding total bacteria counts tended to be associated with the incidence of SCM. Bedding counts of coliforms positively associated with the prevalence of SCM. Our results can support decision-makers in the dairy industry seeking strategies for bedding management and mastitis control.

Development of organic-inorganic hybrid antimicrobial materials by mechanical force and application for active packaging

The loading and delivery of nanomaterials have attracted considerable interest. In this study, the organic-inorganic hybrid antimicrobial materials of clove essential oil (CEO) intercalated organic montmorillonite (MMT) were prepared using a facile and novel cryogenic ball milling technology and characterized. Fourier transform infrared spectroscopy, powder X-ray diffraction, and transmission electron micrograph analysis confirmed the successful preparation of the hybrid antimicrobial materials, and thermogravimetric analysis further demonstrated the thermal stability. In a novel application, hybrid antimicrobial materials were extruded with thermoplastic starch (TPS), polylactic acid (PLA), and poly (butylene adipate-co-terephthalate) (PBAT) to prepare active packaging films. The film release experiments showed that the CEO in the film, containing hybrid antimicrobial materials, was in a sustained release state, and its equilibrium release was higher than that of other films. The prepared films were used to preserve fresh abalone （Haliotis discus hannai Ino）, physicochemical changes and microbiological counts were determined. The results showed that the shelf life of fresh abalone packed in films containing hybrid antimicrobial materials was extended by 3–5 days, indicating that cryogenic ball milling is a simple and effective method for preparing organic-inorganic hybrid antimicrobial materials for active packaging.

Influence of the Slaughter Method on the Hygienic Quality of Beef Cattle Meat and Animal Welfare Biomarkers.

The main objective of this research was to carry out a comparative study between different types of slaughter in beef cattle with and without stunning. In addition, the hygienic quality of the obtained meat was determined through microbiological analysis and the animal welfare at the time of slaughtering was assessed by means of physiological parameters. A total of 52 blood samples collected at the slaughterhouse during slaughter (10 for each type of slaughter: regular, halal, and halal with stunning; 10 at the time of resting; and 12 from rotating box slaughter) were analysed for physiological parameters indicating animal welfare status, namely, glucose, cortisol, lactate dehydrogenase (LDH) and creatine kinase (CK). In addition, the meat from 30 of the above animals was analysed for aerobic mesophilic bacteria, enterobacteria and coliforms. Moreover, a radiological study of the possible skull damage due to the non-penetrative captive bolt used at the time of stunning in the halal rite slaughter was carried out. A significance difference (p < 0.05) in the microbiological counts per type of slaughter was observed. It was proven that the amounts of glucose, LDH, CK, and cortisol in plasma were influenced (p < 0.05) by the type of slaughter. The halal rite slaughter using stunning with a non-penetrative captive bolt resulted in the best hygienic quality of meat and obtained the lowest values for all animal welfare biomarkers.

Curcumin‐containing microencapsulated antioxidant composite paper for cake preservation

SummaryThe cost‐effective strategy of using paper‐based materials as alternatives to plastics can aid in reducing environmental stress. Herein, a novel green low‐carbon paper‐based food‐packaging composite was synthesised: curcumin microcapsule‐polyvinyl alcohol/beeswax/chitosan (CURM‐PVA/BW/CS) composite papers. Scanning electron microscopy and X‐ray diffraction results confirmed that curcumin was successfully encased and adhered to the paper. The water vapour permeability and oxygen transmission rate of the composite papers were 42.28 g (m2·d)−1 and 3.41 cm3 (m2·d·atm)−1, respectively. The antioxidant capacity of paper was 93.53%, and the mechanical properties were effectively improved by adding natural antioxidants to the coating. Finally, cakes were packaged in the biodegradable 1%CURM‐PVA/BW/CS papers exhibited optimal preservation during the storage period, based on the characterisation of the cake peroxide value, acid value and microbiological count. Overall, the CURM‐PVA/BW/CS paper showed great potential for food packaging.

Construction of a simple dual-mode ATP-sensing system for reliable fish freshness evaluation

Adenosine triphosphate (ATP), the main carrier of chemical energy, plays a key role in various biochemical reactions such as cellular metabolism. Currently, ATP levels are considered important indicators of microbial content in food safety, and food freshness can be determined by detecting ATP content. Some ATP sensing strategies have been applied to evaluate food freshness. However, cumbersome nanomaterial preparation, low sensitivity, and low reliability hamper their widespread application. Herein, a simple, high-performance, and reliable dual-mode sensing system based on hemin-G-quadruplex (G4) DNAzyme was established to detect ATP and assess fish freshness. Two nucleic acid probes, including subunits of the hemin-G4 DNAzyme in inactive structures and anti-ATP aptamer, self-assemble upon the input of ATP into the active hemin-G4 DNAzyme unit. The generated DNAzyme acts as a biocatalyst for colorimetric or fluorescent readout of the sensing process. The colorimetric and fluorescent dual-mode sensing system enables highly sensitive and reliable analysis of target ATP with detection limits of 71 nM and 73 nM, respectively. Moreover, the biosensor exhibited good selectivity for differentiating ATP from other interfering analytes. The proposed system was used to detect ATP in perch samples, and a linear correlation between ATP level and microbial content was confirmed. The established ATP-sensing system reliably evaluated fish freshness. Notably, in comparison with microbiological counts, the proposed DNAzyme-based dual-mode strategy for freshness evaluation is facile, highly efficient, and cost-effective, thus providing a promising method for food safety and quality monitoring.

Performance, Egg Quality, and Immunity of Laying Hens due to Natural Carotenoid Supplementation: A Meta-Analysis.

Brazil is considered as a great broiler feet exporter, especially for the Chinese trade. Contact lesions at the tibiotarsal region are responsible for economic losses and there is no model for its classification, thereby this study presents a fast and practical grade system to be used in the poultry industry and proposes these lesion characterizations into three different grades. For this, correlation was made between macroscopic, histological findings and microbiological quantification (Escherichia coli, Staphylococcus spp., Streptococcus spp. and sulphite-reducing clostridia) from contact lesions in the tibiotarsal region of 112 broiler carcasses, divided in four groups (n=28), accordingly to the lesion’s intensity. There were no significant differences in microbiological quantification among the groups (p>0.05) except for the grade 3 group, as grade 1 and 2 lesions were in the early stages and histopathological changes such as ulceration were not observed. In grade 3 lesion group, it was observed bacterial cocci grume and ulceration at the articular region and significantly higher microbiological count (p<0.05) for E. coli and Staphylococcus spp. In conclusion, the visual standard proposed in this work, correlated and confirmed by the histopathologic, and microbiologic characterization, allows to precise and fast ascertainment of the contact lesion grade in the tibiotarsal regions of broiler carcasses. Moreover, it should be highlighted that grades 1 and 2 alterations are not caused by an inflammatory process caused by pathogenic agents and should not be considered a public health risk.

Dithiotreitol pre-treatment of synovial fluid samples improves microbiological counts in peri-prosthetic joint infection

PurposeSynovial fluid cultures of periprosthetic joint infections (PJI) may be limited by bacteria living in the fluids as biofilm-aggregates. The antibiofilm pre-treatment of synovial fluids with dithiotreitol (DTT) could improve bacterial counts and microbiological early stage diagnosis in patients with suspected PJI.MethodsSynovial fluids collected from 57 subjects, affected by painful total hip or knee replacement, were divided into two aliquots, one pre-treated with DTT and one with normal saline. All samples were plated for microbial counts. Sensitivity of cultural examination and bacterial counts of pre-treated and control samples were then calculated and statistically compared.ResultsDithiothreitol pre-treatment led to a higher number of positive samples, compared to controls (27 vs 19), leading to a statistically significant increase in the sensitivity of the microbiological count examination from 54.3 to 77.1% and in colony-forming units count from 1884 ± 2.129 CFU/mL with saline pre-treatment to 20.442 ± 19.270 with DTT pre-treatment (P = 0.02).ConclusionsTo our knowledge, this is the first report showing the ability of a chemical antibiofilm pre-treatment to increase the sensitivity of microbiological examination in the synovial fluid of patients with peri-prosthetic joint infection. If confirmed by larger studies, this finding may have a significant impact on routine microbiological procedures applied to synovial fluids and brings further support to the key role of bacteria living in biofilm-formed aggregates in joint infections.

Effect of Chitosan and Alginate-Based Edible Membranes with Oregano Essential Oil and Olive Oil in the Microbiological, Physicochemical and Organoleptic Characteristics of Mutton.

Edible chitosan or alginate coatings and their combinations with oregano essential oil or olive oil, have been examined for their effect on the microbiological, physicochemical and organoleptic characteristics of mutton. The results indicated that these edible coatings can contribute to maintaining good quality characteristics and extending mutton shelf-life. The total mesophilic counts in mutton ranged from 3.48 to 8.00 log10 CFU/g, the total psychrophilic counts from 4.00 to 9.50 log10 CFU/g, the B. thermosphacta counts from 2.30 to 7.77 log10 CFU/g and the lactic acid bacteria counts from 2.00 to 5.85 log10 CFU/g. Chitosan coatings significantly (p < 0.05) reduced the total mesophilic, the total psychrophilic (1-2 log10 cfu/g), the B. thermosphacta and the lactic acid bacteria counts in mutton. Alginate exhibited a lower L* value and a higher a* value and chroma compared with the control and chitosan lots. No significant differences were observed in the chemical composition of meat pieces among the experimental groups. Oregano oil positively affected the sensory attributes of meat. The most favourable combination, based on the microbiological counts, the organoleptic characteristics and the shelf-life extension of mutton, was that of chitosan with oregano essential oil.

Case of Preferential Selection of Attribute over Variable Control Charts in Trend Analysis of Microbiological Count in Water

Case of Preferential Selection of Attribute over Variable Control Charts in Trend Analysis of Microbiological Count in Water

Effect of high pressure processing on biogenic amines content in skin-packed beef during storage

The effect of the high pressure processing (300, 400 and 500 MPa, 5 min, 5 °C) on the microbiological quality and biogenic amines content in skin-packed beef during refrigerated storage (4-6 °C, 56 days) was evaluated. The correlation significance between microbiological counts and biogenic amines contents was also determined. The higher the applied pressure was, the slower bacteria growth, mainly Enterobacteriaceae and lactic acid bacteria, in skin-packed beef was observed. The use of HPP significantly (P ≤ 0.05) inhibited the formation of cadaverine (CAD), putrescine (PUT) and tyramine (TYM) in beef and this was more effective the higher the pressure was applied. Based on significance and height of correlation coefficients, the BAI (Biogenic Amines Index) value and the level of CAD and PUT would be the most useful indicators of the quality deterioration of skin-packed beef subjected to HPP in the range of 300–500 MPa.

RHEOLOGICAL, TEXTURAL AND PHYSICOCHEMICAL PROPERTIES OF BUCKWHEAT SOURDOUGH BREAD PREPARED WITH DIFFERENT LACTIC ACID BACTERIA STRAINS

The aim of this study is to make buckwheat sourdough with three different lactic acid bacteria (LAB) strains and to determine its quality on wheat bread. After 24 hours fermentation, microbiological count, pH and TTA values and viscoelastic properties of buckwheat sourdoughs were examined. While the Limosilactobacillus fermentum 29GT-19 strain providing the highest value (109 CFU / g) in microbiological count, it was not observed in the chemically acidified control buckwheat dough. pH values changed from 3.8 to 4.6. The elastic modulus (G´) of all sourdoughs was found to be higher than the viscous modulus (G´´) and a positive correlation was found between rheology graph with increase acidity. The prepared buckwheat doughs were then added to wheat flour as 10% and 20% (w / w). At the same time, commercial yeast bread was made as control bread. In the chemical composition of the breads, Levilactobacillus brevis KCO-48 (dough with 20% buckwheat) sourdough bread had the highest ash content (1.26%) and moisture content. But mostly, the ash content of the breads was low, and the amount of moisture was higher than commercial yeast bread. The breads were generally found to have high crumb hardness (from 11.48 N to 31.34 N). As the concentration of buckwheat sourdough increased, the hardness of the bread increased. The lowest hardness (5.21 N) was determined in commercial yeast wheat bread. The results of this study have had a positive effect on the quality of wheat bread of buckwheat sourdough.

Preliminary evidence supporting a new enzymatic debridement product for use in chronic wounds.

A new recombinant proteolytic enzyme, isolated from maggot saliva, with fibrinolytic action has been investigated through a series of non-clinical toxicology and in-vitro/in-vivo pharmacology studies to explore its potential safety and efficacy as an enzymatic debridement agent for use in chronic wounds. Studies indicate that the enzyme has a good safety profile. When locally administered, it is not detrimental to wound healing, is non-sensitising and is rapidly inactivated in the systemic circulation. Adverse effects are limited, at very high concentrations, to transient erythema at the site of application. In-vitro testing indicates that the enzyme, whilst selective for fibrin, has additional proteolytic action against collagen and elastin, with enzymatic action for all three substrates being dose dependent. In-vivo, we used an established MRSA biofilm model, in which microbiological counts were used as a surrogate for debridement efficacy. Here, we showed that higher concentrations of the enzyme in a formulated proprietary gel, significantly reduced MRSA counts over a period of 2 to 14 days, and significantly improved the vascularity of the wound at 14 days. Together, these data support the potential for this maggot-derived proteolytic enzyme as a clinically effective debriding agent.

Microbiological total viable count detection based on tunable diode laser wavelength-modulation spectroscopy

Total viable counts (TVC) are vitally critical to health management, and the quantitative testing of TVC is an essential part of the microbiological testing process. This work demonstrates the rapid and accurate measurement of microbiological TVC using a wavelength-modulated tunable diode laser based on absorption spectroscopy (WM-TDLAS). To detect CO2 in sealed culture bottles, a tunable distributed feedback (DFB) laser with a central wavelength of 2004 nm was employed as the light source. Experiments with Salmonella show that the technique can generate effective second harmonic signals, allowing the detection limit to be 71.8 ppm. Simultaneously, the optimal parameters for measuring the growth curve of Salmonella at various concentrations were determined to establish the standard curve used to calculate the TVC. When compared to the conventional method ISO 4833:2013, the new detection method illustrates good correlation (R2 = 0.98), and the results can be obtained within the detection range of 1–8 log10(CFU/mL) in 4–12 h, saving at least 36 h. Thus, it was shown that WM-TDLAS is a user-friendly, non-invasive technique that allows rapid and accurate assessment of TVC.

Photobiomodulation Therapy Improves Inflammatory Responses by Modifying Stereological Parameters, microRNA-21 and FGF2 Expression.

Introduction: Photobiomodulation treatment (PBMT) is a relatively invasive method for treating wounds. An appropriate type of PBMT can produce desired and directed cellular and molecular processes. The aim of this study was to investigate the impacts of PBMT on stereological factors, bacterial count, and the expression of microRNA-21 and FGF2 in an infected, ischemic, and delayed wound healing model in rats with type one diabetes mellitus. Methods: A delayed, ischemic, and infected wound was produced on the back skin of all 24 DM1 rats. Then, they were put into 4 groups at random (n=6 per group): 1=Control group day4 (CGday4); 2=Control group day 8 (CGday8); 3=PBMT group day4 (PGday4), in which the rats were exposed to PBMT and killed on day 4; 4=PBMT group day8 (PGday8), in which the rats received PBMT and they were killed on day 8. The size of the wound, the number of microbial colonies, stereological parameters, and the expression of microRNA-21 and FGF2 were all assessed in this study throughout the inflammation (day 4) and proliferation (day 8) stages of wound healing. Results: On days 4 and 8, we discovered that the PGday4 and PGday8 groups significantly improved stereological parameters in comparison with the same CG groups. In terms of ulcer area size and microbiological counts, the PGday4 and PGday8 groups performed much better than the same CG groups. Simultaneously, the biomechanical findings in the PGday4 and PGday8 groups were much more extensive than those in the same CG groups. On days 4 and 8, the expression of FGF2 and microRNA-21 was more in all PG groups than in the CG groups (P<0.01). Conclusion: PBMT significantly speeds up the repair of ischemic and MARS-infected wounds in DM1 rats by lowering microbial counts and modifying stereological parameters, microRNA-21, and FGF2 expression.