The polar regions are among the most biologically constrained in the world, characterized by cold temperatures and reduced liquid water. These limitations make them among the most climate-sensitive regions on Earth. Despite the overwhelming constraints from low temperatures and resource availability, many polar ecosystems, including polar deserts and tundras across the Arctic and Antarctic host uniquely diverse microbial communities. Polar regions have warmed more rapidly than the global average, with continued warming predicted for the future, which will reduce constraints on soil microbial activity. This could alter polar carbon (C) cycles, increasing CO2 emissions into the atmosphere. The objective of this study was to determine how increased temperature and moisture availability impacts microbial respiration in polar regions, by focusing on a diversity of ecosystem types (polar desert vs. tundra) that are geographically distant across Antarctica and the Arctic. We found that polar desert soil microbes were co-limited by temperature and moisture, though C and nitrogen (N) mineralization were only stimulated at the coldest and driest of the two polar deserts. Only bacterial biomass was impacted at the less harsh of the polar deserts, suggesting microbial activity is limited by factors other than temperature and moisture. Of the tundra sites, only the Antarctic tundra was climate-sensitive, where increased temperature decreased C and N mineralization while water availability stimulated it. The greater availability of soil resources and vegetative biomass at the Arctic tundra site might lead to its lack of climate-sensitivity. Notably, while C and N dynamics were climate-sensitive at some of our polar sites, P availability was not impacted at any of them. Our results demonstrate that soil microbial processes in some polar ecosystems are more sensitive to changes in temperature and moisture than others, with implications for soil C and N storage that are not uniformly predictable across polar regions.