Microalgal Components Research Articles

Benzo(b)fluoranthene and benzo(k)fluoranthene removal by microalgae in the presence of triazine herbicides: Matrix solid-phase dispersion and solid-phase extraction (MSPD/SPE) for HPLC-UV analysis of the different culture components

Over the last decade, human activities in the industrial and agricultural sectors have significantly increased the concentration of persistent and harmful pollutants in aquatic ecosystems. The use of microorganisms is a green strategy for the bio-removal of certain contaminants. However, other pollutants in the same ecosystems can reduce their degrading activity and even affect their survival. Therefore, this study aimed to evaluate the efficiency of benzo(b)fluoranthene (BbF) and benzo(k)fluoranthene (BkF) removal by Selenastrum capricornutum in the presence of triazine herbicides, compounds mainly used in broadleaf weeds. The interest of this work focused on identifying in which of the microalgal components the degrading activity is best evidenced and affected. For this purpose, the use of solid-phase extraction (SPE) and matrix solid-phase dispersion (MSPD) extraction procedures and HPLC-UV analysis allowed the BbF and BkF trace quantification in biomass, liquid medium, and cell lysate separately from cultures exposed to these polycyclic aromatic hydrocarbons (PAHs) alone or with herbicides. The recovery percentages were between 78 and 94 %, good linearity (r2 ≈ 0.99), precision values measured as RSD < 15 %, and limits of detection (LOQs) at levels of ng mL−1 and ng mg−1 were obtained. The individual PAH amounts measured in the components of microalgae cultures show similar removal kinetics (removal percentages: 82–89 %). Likewise, the analysis demonstrated that the removal of PAHs is not affected in the presence of triazine herbicides (atrazine and cyanazine) and with similar removal percentages (79–86 %) compared to those cultures exposed to individual PAHs (74–83 %). These results support the possible real-world applications of PAH removal by extracts from S. capricornutum in aquatic environments contaminated with PAHs and near agriculture areas where triazine herbicides are used.

How Daphnia magna Defends Itself against Predators: Mechanisms and Adaptations in a Freshwater Microcosm

The freshwater water flea (Daphnia magna Straus, 1820) is prey for numerous predators. Yet it possesses a wide range of strategies to defend itself against predation. The aim of this work is to investigate the defensive mechanisms employed by D. magna to reduce predation by the coelenterate Hydra viridissima, and two planarians, Polycelis felina and Dugesia gonocephala. To do this, we used a freshwater microcosm. An additional aim is to investigate interactions with the presence of the isolated endosymbiotic algae from green hydra, thus combining and observing the interaction of the zooplankton and microalgal component. Each experiment included five replicates (13.5 °C, 25 °C), in crystallizing glass containers (60 mL volume, 60 mm diameter, 35 mm height), including satiated (fed with larvae of Artemia salina) and starved predators, respectively (one or five individuals of a particular predator species in one microcosm). As the isolated microalgae are unique, we tracked the following three mechanisms of Daphnia defense for the first time including precisely this microalgal component: (i) grouping (visual magnification), i.e., two or more Daphnia holding together; (ii) the phenomenon of overproduction, i.e., any number of Daphnia in one container above the 10 initially added individuals; and (iii) accelerated movement (“bullet movement”), i.e., high-speed movements in particular microcosms. The results provide new information for a better understanding of the interspecific relationships in systems that include both zooplankton and microalgal components.

Study of the interaction mechanism between the components of microalgae by thermal behaviors and pyrolysis characteristics

There is significant effect on thermal behaviors pyrolytic characteristics of the interactions between microalgal components. In order to understand the reaction process, interaction mechanism, and better utilization of microalgae, co-pyrolysis of microalgal model compounds (soybean protein (PT), sunflower oil (OL) and sucrose (SC)) was carried out with TG-FTIR and Py-GC/MS. The results showed that co-pyrolysis of PT and SC significantly changed the thermal behaviors, and accelerated decomposition at lower temperature to generate CO, CO2 largely. And it could promote the generation of linear O-compounds (such as acetic acid), and inhibit the formation of N-heterocycles and furans. Moreover, OL co-pyrolysis with PT or SC could significantly promote the formation of long-chain acid and inhibit the generation of aliphatics. Meanwhile, co-pyrolysis of PT and OL inhibited the generation of N-heterocycles. And co-pyrolysis of SC and OL accelerated the generation of furans. Furthermore, co-pyrolysis of model compounds reduced the average activation energy of the pyrolysis process, and changed the reaction kinetic model.

Microalgal-induced remediation of wastewaters loaded with organic and inorganic pollutants: An overview

The recent surge in industrialization has intensified the accumulation of various types of organic and inorganic pollutants due to the illegal dumping of partially and/or untreated wastewater effluents in the environment. The pollutants emitted by several industries pose serious risk to the environment, animals and human beings. Management and diminution of these hazardous organic pollutants have become an incipient research interest. Traditional physiochemical methods are energy intensive and produce secondary pollutants. So, bioremediation via microalgae has appeared to be an eco-friendly and sustainable technique to curb the adverse effects of organic and inorganic contaminants because microalgae can degrade complex organic compounds and convert them into simpler and non-toxic substances without the release of secondary pollutants. Even some of the organic pollutants can be exploited by microalgae as a source of carbon in mixotrophic cultivation. Literature survey has revealed that use of the latest modification techniques for microalgae such as immobilization (on alginate, carrageena and agar), pigment-extraction, and pretreatment (with acids) have enhaced their bioremedial potential. Moreover, microalgal components i.e., biopolymers and extracellular polymeric substances (EPS) can potentially be exploited in the biosorption of pollutants. Though bioremediation of wastewaters by microalgae is quite well-studied realm but some aspects like structural and functional responses of microalgae toward pollutant derivatives/by-products (formed during biodegradation), use of genetic engineering to improve the tolerance of microalgae against higher concentrations of polluatans, and harvesting cost reduction, and monitoring of parameters at large-scale still need more focus. This review discusses the accumulation of different types of pollutants into the environment through various sources and the mechanisms used by microalgae to degrade commonly occurring organic and inorganic pollutants.

Microalgal Growth in Aquaculture Effluent: Coupling Biomass Valorisation with Nutrients Removal

Natural resources are becoming increasingly scarce, and the need to control their consumption and recycle their use is growing. Water is one of the essential resources for human survival. Therefore, there has been an increasing interest in ways to save, recycle and treat water supplies. Aquaculture is one of the most polluting activities as it produces a significant wastewater volume, which needs proper treatment before being discharged into the environment or recycled. Microalgae are a potential solution for wastewater treatment. Due to their numerous advantages, the use of microalgal biomass is being studied, and, at present, there is already a market and room for profit in the sale of microalgal components in various forms, such as animal and human supplements. From a biorefinery point of view, it is important to take advantage of all the qualities and benefits that microalgae have by combining their great capacity to treat wastewater and exploit the produced biomass, analysing its composition for subsequent valorisation, for example. In this study, Chlorella vulgaris was used to treat aquaculture wastewater from a trout farm aquaculture facility, and the treatment efficiency was evaluated. To valorise the resulting biomass, its composition was also assessed. C. vulgaris successfully grew in the effluent with growth rates of 0.260 ± 0.014 d−1 and with average productivity of 32.9 ± 1.6 mg L−1 d−1. The achieved removal efficiencies were 93.5 ± 2.1% for total nitrogen, 98.0 ± 0.1% for nitrate-nitrogen and 92.7 ± 0.1% for phosphate-phosphorus. Concerning biomass composition, the lipids (15.82 ± 0.15%), carbohydrates (48.64 ± 0.83%), and pigment contents (0.99 ± 0.04% for chlorophyll a + b and 0.21 ± 0.04% for carotenoids) were similar to the values of similar studies. However, the protein content obtained (17.93 ± 1.21%) was lower than the ones mentioned in the literature.

Oxidative torrefaction of microalga Nannochloropsis Oceanica activated by potassium carbonate for solid biofuel production

Oxidative torrefaction is a promising way for biomass upgrading and solid biofuel production. Alkali metals are considered to be efficient activators for enhancing biofuel upgrading during the thermal reaction process. Herein, the microalga Nannochloropsis Oceanica is selected as the feedstock for assessing potassium carbonate activated effect on solid biofuel production through oxidative torrefaction. The potential of potassium carbonate on microalgal biofuel properties upgrading is deeply explored. SEM observation and BET analysis show that torrefied microalgae can be transformed from a spherical structure with wrinkles to smaller particles with larger surface areas and higher total pore volumes, implying that potassium carbonate is a promising porogen. Moreover, potassium carbonate can significantly change the DTG curve at the temperatures of 250 °C and 300 °C from one peak to two peaks, inferring that the activated effect of potassium carbonate occurs on the torrefied microalgae. 13C NMR analysis reveals that the microalgal components significantly change as the torrefaction severity increases, with the decomposition of carbohydrate and protein components. When the potassium carbonate ratio increases from 0:1 to 1:1, the graphitization degree increase from 3.065 to 1.262, along with the increase in the HHV of solid biofuel from 25.024 MJ kg−1 to 31.890 MJ kg−1. In total, this study has comprehensively revealed the activated effect of potassium carbonate on improving the properties of microalgal solid biofuel.

Efficient Fractionation of Lipids in a Multiproduct Microalgal Biorefinery by Polymers and Ionic Liquid-Based Aqueous Two-Phase Systems

For a multiproduct microalgal biorefinery, most of the cell components should be extracted and fractionated. This work investigates the fractionation of lipids from other microalgal components (pigments, proteins, carbohydrates) using polymers and IL solutions in aqueous two-phase systems (ATPS). The microalgal lipids poorly migrated to the aqueous phases of ATPS and were recovered (97% of the total fatty acids) in a third phase (interphase) formed between the top and bottom phases. Studies with canola oil and purified phospholipids suggest that the high amount of oil, phospholipids, and other natural emulsifiers present in the microalgae mixed with the high amount of water in the ATPS form an emulsion which is difficult to fractionate. However, a solution of polypropylene glycol 400 (25% w/w) displaced 73% of lipids in an immiscible layer which was easy to recover. When combining this approach with a subsequent ATPS, most of the microalgae biomolecules (lipids, proteins, pigments, carbohydrates) could be fractionated in a three-step mild separation concept.

Microbiome in Cladonia squamosa Is Vertically Stratified According to Microclimatic Conditions.

Lichens are miniature ecosystems that contain fungi, microalgae, and bacteria. It is generally accepted that symbiosis between mycobiont and photobiont and microbial contribution to the ecosystem support the wide distribution of lichens in terrestrial ecosystems, including polar areas. The composition of symbiotic components can be affected by subtle microenvironmental differences within a thallus, as well as large-scale climate differences. In this study, we investigated fine-scale profiles of algal, fungal, and bacterial compositions through horizontal and vertical positions of the Antarctic lichen Cladonia squamosa colonies by next-generation sequencing of the nuclear large subunit rRNA gene (nucLSU) of eukaryotes and the 16S rRNA gene of bacteria. Apical parts of thalli were exposed to strong light, low moisture, and high variability of temperature compared with basal parts. Microbial diversity increased from apical parts to basal parts of thalli. Asterochloris erici was the major photobiont in apical positions of thalli, but other microalgal operational taxonomic units (OTUs) of Trebouxiophyceae and Ulvophyceae were major microalgal components in basal positions. Photochemical responses of algal components from apical and basal parts of thalli were quite different under variable temperature and humidity conditions. Several fungal OTUs that belonged to Arthoniomycetes and Lecanoromycetes, and diverse bacterial OTUs that belonged to Alphaproteobacteria, Acidobacteria_Gp1, and candidate division WPS-2 showed a clear distribution pattern according to their vertical positions within thalli. The overall lichen microbiome was significantly differentiated by the vertical position within a thallus. These results imply that different microclimate are formed at different lichen thallus parts, which can affect microbial compositions and physiological responses according to positions within the thalli.

Multistep Fractionation of Microalgal Biomolecules Using Selective Aqueous Two-Phase Systems

We aim to develop liquid–liquid extraction processes for the fractionation of microalgal components (proteins, pigments, lipids, and carbohydrates). The partitioning behavior of microalgal pigments...

The Protein Extract of Chlorella minutissima Inhibits The Expression of MMP-1, MMP-2 and MMP-9 in Cancer Cells through Upregulation of TIMP-3 and Down Regulation of c-Jun.

Objective Considering the bioactivities exhibited by microalgae, the effect of protein extract of Chlorella minutissimma (CP extract) was investigated on the expression of human matrix metalloproteinases-1 (MMP-1) in the breast cancer cell line MDA-MB231, and that of MMP-2 and -9 in hepatocellular cancer cell line HepG2 at different expression levels. The study aimed identification and analysis of inhibitory activity of microalgal components extracted from Chlorella minutissima against human MMPs. Materials and Methods In this experimental study, we analysed the effect of Chlorella extracts on MMP-1, -2, and -9 expression at various levels. Gelatin zymography was performed to study the inhibitory effect of Chlorella exracts on human gelatinases at the activity level, followed by western blotting to analyse the expression of all three MMPs at the protein level. The similar effect at the mRNA level along with the probable mechanism underlying inhibition of MMPs was assessed using real-time polymerase chain reaction (PCR). Results The results reveal that the treatment with CP extract decreased the mRNA expression of MMP-1, MMP-2, and MMP-9 by 0.26-, 0.29-, and 0.40-fold, respectively, at 20 μg/ml concentration as well as inhibited the activity of MMP-2 and MMP-9 by 37.56 and 42.64%, respectively, at 15 μg/ml concentration. Additionally, upregulated mRNA expression of tissue inhibitor of metalloproteinases-3 (TIMP-3) by 1.68-fold was seen in HepG2 cells at 20 μg/ml concentration treatment group. However, CP extract did not induce any change in the mRNA expression of the TIMP-1, -2 and -4 in HepG2 and TIMP-1, -2, -3 and -4 in MDA-MB231 cells. Activator protein-1 (AP-1)-dependent c-Jun-mediated transcriptional regulation of MMP-1, -2, and -9 was also studied to elucidate the appropriate mechanism involved in the inhibition of MMPs. ConclusionThe CP extract successfully inhibited MMP-1, -2, and -9 at different expression levels through TIMP-3 upregulation and c-Jun downregulation.

Hydrothermal liquefaction of microalgae to produce biofuels: state of the art and future prospects

The article presents a review of the state of the art and lines of research on hydrothermal liquefaction (HTL) of microalgae (MA). The main advantages of this technology for production of biofuel are that it does not require predrying of the feedstock and ensures a relatively high product yield—the ratio of the end product weight to the feedstock weight—owing to the fact that all the microalgal components, viz., lipids, proteins, and carbohydrates, are converted into biofuel. MA hydrothermal liquefaction is considered to be a promising technology for conversion of biomass and is a subject of a series of research studies and, judging by the available publications, the scope of research in this field is expanding currently. However, many significant problems remain unsolved. In particular, an active searched is being conducted for suitable strains that will ensure not only a high lipid yield—necessary to convert microalgae into biodiesel—but also higher biomass productivity and a higher biofuel yield; the chemical reactions that occur during the hydrothermal treatment are being studied; and the effect of significant process variables, such as temperature, heating rate, holdup time at the maximum temperature, biomass concentration in the water suspension, biochemical and elemental compositions of the microalgae, use of catalysts, etc., on the liquefaction processes is being studied. One of the urgent tasks is also the reduction of the nitrogen content in the resulting biofuel. Studies aimed at the development of a continuous process and rational heat-processing plants for thermal microalgal conversion are being conducted to increase the energy efficiency of the HTL process, in particular, to provide the heat recovery and separation of the end product.

Pyrolysis mechanism of microalgae Nannochloropsis sp. based on model compounds and their interaction

Pyrolysis is one of important pathways to convert microalgae to liquid biofuels and key components of microalgae have different chemical composition and structure, which provides a barrier for large-scale microalgae-based liquid biofuel application. Microalgae component pyrolysis mechanism should be researched to optimal pyrolysis process parameters. In this study, single pyrolysis and co-pyrolysis of microalgal components (model compounds castor oil, soybean protein and glucose) were conducted to reveal interaction between them by thermogrametric analysis and bio-crude evaluation. Castor oil (model compound of lipid) has higher pyrolysis temperature than other model compounds and has the maximum contribution to bio-crude formation. Bio-crude from soybean protein has higher N-heterocyclic compounds as well as phenols, which could be important aromatic hydrocarbon source during biorefineries and alternative aviation biofuel production. Potential interaction pathways based on model compounds are recommended including further decomposition of Maillard reaction products (MRPs) and surfactant synthesis, which indicate that glucose played an important role on pyrolysis of microalgal protein and lipid components. The results should provide necessary information for microalgae pyrolysis process optimization and large-scale pyrolysis reactor design.

Gasification kinetics of raw and wet-torrefied microalgae Chlorella vulgaris ESP-31 in carbon dioxide

This study aims at investigating the gasification behavior and kinetics of microalga Chlorella vulgaris ESP-31 before and after wet torrefaction. The raw and wet-torrefied microalgae were first gasified in a thermogravimetric analyzer under a continuous CO2 flow. Thereafter, the obtained thermogravimetric data were modeled for kinetic study, employing a seven-parallel-reaction mechanism. The decomposition of the microalgae in CO2 shows two reactive stages: devolatilization with two peaks and gasification with a peak accompanied by a shoulder, and the thermal decomposition of components in the samples can be clearly identified. Increasing wet torrefaction temperature lowers the height of the major devolatilization peak but enhances the height of the minor one. Moreover, the kinetic evaluation reveals that wet torrefaction affects most of the kinetic parameters of the microalgal components. Furthermore, wet torrefaction temperature influences the kinetic parameters of carbohydrate and lipid, but not on those of protein, “others”, and chars.

Pyrolysis characteristics and pathways of protein, lipid and carbohydrate isolated from microalgae Nannochloropsis sp.

Microalgal components were isolated gradually to get lipid-rich, protein-rich and carbohydrate-rich components. The aim of this work was to study pyrolysis mechanism of microalgae by real isolated real algae components. Thermogrametric analysis (DTG) curve of microalgae was fitted by single pyrolysis curves of protein, lipid and carbohydrate except special zones, which likely affected by cell disruption and hydrolysis mass loss. Experimental microalgae liquefaction without water index N was 0.6776, 0.3861 and 0.2856 for isolated lipid, protein and carbohydrate. Pyrolysis pathways of lipid are decarboxylation, decarbonylation, fragmentation of glycerin moieties and steroid to form hydrocarbons, carboxylic acids and esters. Pyrolysis pathways of protein are decarboxylation, deamination, hydrocarbon residue fragmentation, dimerization and fragmentation of peptide bonds to form amide/amines/nitriles, esters, hydrocarbons and N-heterocyclic compounds, especially diketopiperazines (DKPs). Pyrolysis pathways of carbohydrate are dehydrated reactions and further fragmentation to form ketones and aldehyde, decomposition of lignin to form phenols, and fragmentation of lipopolysaccharides.

Sulfate-reducing bacteria in the skeleton of the massive coral &lt;i&gt;Goniastrea aspera&lt;/i&gt; from the great barrier reef

Coral skeletons harbor diverse assemblages of endolithic microorganisms that often have a profound influence on the ecology and physiology of the coral host. While the cyanobacterial and eukaryotic microalgal components of these endolithic assemblages are well characterized, information on the other components remains scarce. Here, we characterize the endolithic microbial assemblages in the skeleton of the massive coral Goniastrea aspera in both oligotrohic and eutrophic environments. In addition to the green bands typically found in massive coral skeletons, multiple black bands were observed in skeletons from oligotrophic waters. Sequence analysis of the dsrB (dissimilatroy sulfite reductase beta-subunit) gene revealed that colonies with black bands harbor a diverse assemblage of sulfate-reducing bacteria (SRB). In contrast, SRB were not detected in skeletons from eutrophic waters. We conclude that increases in nutrients may have an important effect on the microbial assemblages residing within coral skeletons.

Microalgae-based biorefinery – From biofuels to natural products

The potential for biodiesel production from microalgal lipids and for CO2 mitigation due to photoautotrophic growth of microalgae have recently been recognized. Microalgae biomass also has other valuable components, including carbohydrates, long chain fatty acids, pigments and proteins. The microalgae-based carbohydrates consist mainly of cellulose and starch without lignin; thus they can be ready carbon source for the fermentation industry. Some microalgae can produce long chain fatty acids (such as DHA and EPA) as valuable health food supplements. In addition, microalgal pigments and proteins have considerable potential for many medical applications. This review article presents comprehensive information on the current state of these commercial applications, as well as the utilization and characteristics of the microalgal components, in addition to the key factors and challenges that should be addressed during the production of these materials, and thus provides a useful report that can aid the development of an efficient microalgae-based biorefinery process.

Distribution and Composition of Cyanobacteria and Microalgae Associated with Biological Soil Crusts in the Gurbantunggut Desert, China

In Gurbantunggut Desert, cyanobacterial and microalgal components were characterized within 60 soil samples collected from sand dunes. Fifty-one taxa of cyanobacteria and algae were identified. Without exception, the soils were alkaline, poor in nutrients, and showed large variations in other soil properties. Spatial heterogeneity for distribution of cyanobacteria and microalgae (diversity of morphotypes, species composition, and microbiomass) existed. Compared with other deserts in the world, the Gurbantunggut Desert has a greater diversity of cyanobacterial-microalgal morphotypes. Results from step regression showed that the diversity of morphotype was determined by total P, available P, and soil layer. Filamentous cyanobacteria dominated the community. Microcoleus vaginatus (Vauch.) Gom was the dominant species in most positions on sand dune, while the abundance of other dominant species varied depending on the sand dune position and the soil layer in which they occurred. The microalgal biomass was influenced by the content of Mg, crust type, soil moisture, sunlight, and oxygen concentration. A significant positive relation was found between microalgal biomass and diversity of morphotype. Species composition, diversity of morphotype, and microalgal biomass interacted with each other. The contents of P and Mg ion, soil texture, and soil moisture may be the main factors responsible for cyanobacterial-microalgal distribution.

Confocal laser scanning fluorescence microscopy of Botryococcus alginite from boghead oil shale, Boltysk, Ukraine: selective preservation of various micro-algal components

Confocal laser scanning fluorescence microscopy of Botryococcus alginite from boghead oil shale, Boltysk, Ukraine: selective preservation of various micro-algal components

Confocal laser scanning fluorescence microscopy of Botryococcus alginite from boghead oil shale, Boltysk, Ukraine: selective preservation of various micro-algal components

Confocal laser scanning fluorescence microscopy (LSM) has exceptional potential for resolving micrometer scale morphological details within fluorescing macerals (e.g. alginite) of hydrocarbon source rocks and oil shales. This investigation of well preserved Botryococcus alginites from a Paleogene boghead oil shale from central Ukraine clearly illustrates that LSM can effectively resolve microalgal cellular organization. A dominance of highly resistant, outermost cell walls in Botryococcus confirms that selective preservation was an effective process during kerogen formation. Three dimensional serial section compilations of images taken through Botryococcus alginites reveals a number of cellular features including: (i) micro-layering within resistant outer cell walls; (ii) preservation of resistant, very thin, outer walls of apical cells and; (iii) stacks of successive thimble-shaped layers and funnel-shaped cups which comprise the resistant stalk framework of fossilized compound colonies. LSM also discloses preservation of pairs of ‘reproducing’ Botryococcus cells consisting of outer resistant walls enclosing mainly unstructured, granular organic matter in the ‘cell contents region’. Some serial images from these areas do, however, show evidence for cellular organization and possible selective preservation of possible resistant biomacromolecules derived from aplanosphores or zoospores.