Mg Of Green Tea Extract Research Articles

The Use of Some Polyphenols in the Modulation of Muscle Damage and Inflammation Induced by Physical Exercise: A Review

Food bioactive compounds (FBC) comprise a vast class of substances, including polyphenols, with different chemical structures, and they exert physiological effects on individuals who consume them, such as antioxidant and anti-inflammatory action. The primary food sources of the compounds are fruits, vegetables, wines, teas, seasonings, and spices, and there are still no daily recommendations for their intake. Depending on the intensity and volume, physical exercise can stimulate oxidative stress and muscle inflammation to generate muscle recovery. However, little is known about the role that polyphenols may have in the process of injury, inflammation, and muscle regeneration. This review aimed to relate the effects of supplementation with mentation with some polyphenols in oxidative stress and post-exercise inflammatory markers. The consulted papers suggest that supplementation with 74 to 900 mg of cocoa, 250 to 1000 mg of green tea extract for around 4 weeks, and 90 mg for up to 5 days of curcumin can attenuate cell damage and inflammation of stress markers of oxidative stress during and after exercise. However, regarding anthocyanins, quercetins, and resveratrol, the results are conflicting. Based on these findings, the new reflection that was made is the possible impact of supplementation associating several FBCs simultaneously. Finally, the benefits discussed here do not consider the existing divergences in the literature. Some contradictions are inherent in the few studies carried out so far. Methodological limitations, such as supplementation time, doses used, forms of supplementation, different exercise protocols, and collection times, create barriers to knowledge consolidation and must be overcome.

The use of green tea in the treatment of symptomatic uterine fibroids

Background: Green tea is a natural product, commonly used by women for multiple purposes. Epigallocatechin gallate (EGCG), the major catechin in green tea, exhibits several useful biological effects, including anti-inflammatory, antiproliferative, and antioxidant effects. A study conducted by the US Department of Agriculture reported that green tea has potent anticancer effects against a wide range of human cancer cells. Green tea’s polyphenols are considered responsible for these positive effects, and most notably EGCG has been shown to inhibit key pathways of tumor growth. Objective: Aim of the work was to study the effects of epigallocatechin gallate (EGCG), an extract of green tea on human leiomyoma and quality of life in women with symptomatic uterine fibroids. Patients and methods: A Prospective, sealed envelopes, randomized control trial was conducted including 75 reproductive-age women with symptomatic uterine fibroids (UF) were recruited for this study. All subjects had at least one fibroid lesion ≥ 2 cm³, as confirmed by transvaginal ultrasonography. The subjects had been randomized by SNOSE (Sequentially Numbered Opaque Sealed Envelopes); to oral daily treatment with either 900 mg of green tea extract or only symptomatic treatment for 4 months. The duration of study was about 3 years.

Effects of Green Tea Extract on Atorvastatin Pharmacokinetics in Healthy Volunteers

Green tea catechins were recently reported to inhibit drug transporters such as organic anion-transporting polypeptides (OATPs) and metabolic enzymes, affecting the bioavailability of many drugs. This study aimed to evaluate the clinical significance of the effects of different doses of green tea extract on the pharmacokinetic parameters of atorvastatin and to rationalize the associated interaction mechanism. A randomized, double-blind, three-phase crossover study involving 12 healthy volunteers was performed. Participants received a single dose of atorvastatin 40mg alone (control group), atorvastatin 40mg plus a capsule containing 300mg of dry green tea extract, or atorvastatin 40mg plus a capsule containing 600mg of dry green tea extract. Plasma samples taken from the volunteers were analyzed for atorvastatin using liquid chromatography-tandom mass spectrometry (LC/MS/MS). Compared to atorvastatin alone, the administration of 300mg or 600mg of the green tea extract along with atorvastatin decreased the peak plasma concentration (Cmax) of atorvastatin by 25% and 24%, respectively (P < 0.05), and the area under the plasma concentration-time curve (AUC0-∞) of atorvastatin by 24% and 22%, respectively (P < 0.05). Additionally, administration of 300mg or 600mg of the green tea extract increased the apparent oral clearance (CL/F) of atorvastatin by 31% and 29%, respectively. The time to Cmax (Tmax) and the elimination half-life (t1/2) of atorvastatin did not differ among the three phases. The effects of 600mg of the green tea extract on the pharmacokinetic parameters of atorvastatin were not significantly different from the effects of 300mg of the green tea extract. Green tea extract decreases the absorption but not the elimination of atorvastatin, possibly by inhibiting OATP, albeit not in a dose-dependent manner. Coadministration of green tea extract with atorvastatin may necessitate the monitoring of the plasma concentration of atorvastatin in clinical practice.

Effect of green tea extract on arterial stiffness, lipid profile and sRAGE in patients with type 2 diabetes mellitus: a randomised, double-blind, placebo-controlled trial

Type 2 diabetes mellitus (T2DM) is associated with premature atherosclerosis and arterial stiffening due to the accumulation of advanced glycation end-products in vessel walls. Green tea polyphenols are considered cardio-protective substances. In this randomised double-blind placebo-controlled trial (NCT02627898), we evaluated the effect of Green tea extract on arterial stiffness parameters, lipids, body composition and sRAGE levels. Twenty normotensive patients with T2DM treated with the standard therapy and statins, mean age 53.2 ± 9.4 years and mean BMI 30.1 ± 4.5 kg/m2, were randomised to receive a daily dose of 400 mg of green tea extract (polyphenols ≥90%, EGCG ≥45%) or placebo for 12 weeks. Compared to placebo, administration of green tea extract decreased central augmentation index (–3.05 ± 10.8% vs. 6.7 ± 0.1%, p = .04). These findings suggest that green tea extract could be used as an adjunct to the standard therapy to improve arterial stiffness in T2DM.

Effect of green tea extract on bone mass and body composition in individuals with diabetes

Green tea has shown benefits in diabetes and osteoporosis. The purpose of this article is to evaluate the effect of green tea on glycemic control, body composition and bone mass in diabetic subjects. A double-blind, randomized, controlled clinical trial was conducted with 35 diabetic subjects. Participants received 1120 mg of green tea extract containg 560 mg of polyphenols/day or cellulose. Body composition, bone mass measures and other parameters are evaluated after 10 and 20 weeks. For bone mineral content, a 1.60 g increase in green tea group and a 0.98 g decrease in control group resulted in a difference in mean of 2.58 g between groups approaching significance (p = 0.056). It was observed an increase in PTH after 10 weeks in the green tea group (p = 0.008) with a subsequent reduction at 20 weeks. Thus, green tea may contribute to improvement of bone mineral content in diabetic subjects.

緑茶摂取が有酸素運動時のエネルギ-基質に及ぼす影響

The purpose of this study is to clarify the influence of non-caffeine green tea and green tea with caffeine on energy substrate during exercise using standard carbohydrate metabolism, fat metabolism, blood free fatty acid concentration and catecholamine. The study subjects were consisted of seven healthy female students and placed into two groups: experimental and control groups. The experimental group was given 336mg of glucose, 336mg of green tea extract, 80 mg of caffeine and 336mg of green tea extract and the control group was given the placebo made of water. Then, both groups exercised for 30 minutes at an intensity of 40% VO2max. Using a bicycle ergometer, the concentration of free fatty acid, adrenaline, and noradrenaline and VO₂, VCO₂, and heart rate were measured. The result showed no significant difference in heart rate between each condition. The noradrenaline concentration after exercise was significantly higher than before and after ingestion in each condition (P.05). Non-caffeine green tea increased noradrenaline concentration after ingestion, and caffeinated green tea significantly increased adrenaline concentration and free fatty acid concentration after ingestion (P.05). In addition, total fat metabolism was significantly higher in caffeine green tea condition than in the control condition (P.05). There was no significant difference in non-caffeine green tea conditions, but the total fat metabolism was higher than the control condition. On the other hand, the total carbohydrate metabolism did not differ significantly from each condition.BR It is suggested that catechin contained in green tea promotes fat burning during exercise and fat food burning effect is increased by additional caffeine intake. This indicates that green tea is effective for obesity prevention for standard weight persons.

Effect of Green Tea Extract on Exercise-Induced Oxidative Stress in Obese Men: A Randomized, Double-Blind, Placebo-Controlled, Crossover Study

Background: Green tea contains polyphenol catechins that has been shown to be beneficial to health owing to its antioxidant properties. Objectives: This study investigated the effects of a 14-day ingestion of green tea extract (GTE) on biomarkers of oxidative stress in obese men undergoing resistance exercise (RE). Methods: In a randomized, double-blind, placebo (PL)-controlled, crossover study, ten obese men (age 37.12 ± 5.66 y; height 177 ± 7.7 cm; BMI 32.53 ± 2.52) performed two RE protocols consisting of 3 sets of 6 exercises to exhaustion at 75% of one repetition maximum (1RM) and 2 minute rests between sets, after 14 days of 500 mg of GTE (two capsules per day) or 500 mg PL (two capsules per day) supplementation. Blood samples were collected before (PRE) and immediately after (POST) RE and analyzed for oxidative biomarkers including lipid peroxidation (8-iso PGF2α) and oxidative DNA damage (8-OHdG). Results: Our results showed that a 14-day GTE supplementation did not change lipid peroxidation (P > 0.05); also, no significant alterations for 8-iso PGF2α between GTE and PL treatments after RE were observed (P > 0.05). However, 14-day GTE supplementation significantly reduced oxidative DNA damage as measured by 8-OHdG after RE compared to PL (P < 0.05). Conclusions: The results of this study demonstrated that 14-day supplementation of GTE significantly prevented oxidative DNA damage induced by RE in untrained obese men. However, the potential effects of GTE on oxidative stress after RE in obese people warrants further investigation.

The Effect of short-term aerobic exercise and green tea consumption on MFO, ‎Fatmax, body composition and lipid profile in sedentary postmenopausal ‎‎women

Background: Visceral fat accumulation due to the decrease in estrogen levels, and gaining weight after menopause, increases the risk of metabolic syndrome and cardiovascular diseases in postmenopausal women; While physical activity at Fatmax and green tea consumption are recommended for increasing fat oxidation.&#x0D; Methods: 24 sedentary postmenopausal women were divided into two groups: exercise+supplemnet and exercise+placebo. Exercise tests were performed by gas analyzer device and blood samples were collected after 12 hours of fasting overnight in pre and post test phases. The supplement group had a daily intake of 1200 mg of green tea extract in capsule form. The training Protocol contained two weeks of aerobic exercise at an intensity of about maximum fat oxidation which was performed four times a week and each session took 40 to 50 minutes.&#x0D; Results: After two weeks, the amounts of weight, body mass index, waist to hip ratio, average total carbohydrate oxidation and HDL were significantly decreased in both groups (p≤0.05). The peak oxygen consumption, maximum fat oxidation, Fatmax and the average total fat oxidation increased but were not significant (p&gt;0.05). Triglyceride (P=0.003) and visceral fat levels (P=0.044) only declined significantly in exercise+placebo group and LDL levels (P=0.043) only increased in exercise+supplement, significantly. Fat percentage also decreased in two groups which was not significant (p&gt;0.05).&#x0D; Conclusions: Short-term aerobic exercise at Fatmax, alone and along with green tea consumption, reduced body weight and improved average total carbohydrate oxidation in sedentary postmenopausal women and aerobic exercise with green tea was more effective in reducing triglycerides and visceral fat levels.

Short-term Effects of Green Tea on Blood Pressure, Endothelial Function, and Metabolic Profile in Obese Prehypertensive Women: A Crossover Randomized Clinical Trial

Background: Green tea consumption has been inversely associated with cardiovascular disease (CVD) in epidemiological studies. Although some interventional trials suggest that green tea has beneficial effects on CVD risk factors, such as hypertension and obesity, others have failed to show such benefits.Aims: To evaluate the short-term effects of green tea on blood pressure, endothelial function, metabolic profile, and inflammatory activity in obese prehypertensive women.Methods: This study was a crossover, randomized, double-blind, placebo-controlled clinical trial. Participants were randomly allocated to receive daily 3 capsules containing either 500 mg of green tea extract (GTE) or a matching placebo for 4 weeks, with a washout period of 2 weeks between treatments. Each GTE capsule contained 260 mg of polyphenols. At the beginning and at the end of each treatment, participants were submitted to evaluation of blood pressure (ambulatory blood pressure monitoring, ABPM), endothelial function (Endo-PAT 2000 and cellular adhesion molecules), nutritional parameters, metabolic profile, and biomarkers of inflammation.Results: Twenty women age 41.1 ± 8.4 years completed the study. After 4 weeks of GTE supplementation in comparison with placebo, there was a significant decrease (p < 0.05) in systolic blood pressure at 24 hours (−3.61 ± 1.23 vs 1.05 ± 1.34 mmHg), daytime (−3.61 ± 1.26 vs 0.80 ± 1.57 mmHg), and nighttime (−3.94 ± 1.70 vs 1.90 ± 1.66 mmHg). Changes in diastolic blood pressure and in all other parameters did not present a significant difference between GTE and placebo.Conclusion: The findings of this study suggest that in obese prehypertensive women, short-term daily intake of GTE may decrease blood pressure.

Erratum to: Green tea extract enhances parieto-frontal connectivity during working memory processing

The original version of this article inadvertently contained a mistake. There are incorrect information found under Abstract and Experimental Design Sections. Please see below for the corrections. 1) Current version: Abstract: Using a double-blind, counterbalanced, withinsubject design, 12 healthy volunteers received a milk wheybased soft drink containing 27.5 g of green tea extract or a milk whey-based soft drink without green tea as control substance while undergoing functional magnetic resonance imaging. Should read: Abstract: Using a double-blind, counterbalanced, within-subject design, 12 healthy volunteers received a milk whey-based soft drink containing green tea extract corresponding to 2,75 g/l green tea leaves or a milk whey-based soft drink without green tea as control substance while undergoing functional magnetic resonance imaging 2) Current version: Experimental Design: Participants received either 250 or 500 ml milk whey-based soft drink containing 13.75 and 27.5 g of green tea extract, respectively (Rivella, Rothrist, Switzerland) Should read: Experimental Design: Participants received either 250 or 500 ml milk whey-based soft drink containing 125 and 250 mg of green tea extract corresponding to 2,75 g/l green tea leaves, respectively (Rivella, Rothrist, Switzerland).

Gene-Eden-VIR Decreased Physical and Mental Fatigue in a Post Marketing Clinical Study That Followed FDA Guidelines; Results Support Microcompetition Theory

Objective: The Microcompetition with Foreign DNA theory, proposed by Hanan Polansky in 2003, describes how latent viruses can cause chronic conditions, including fatigue. The Gene-Eden-VIR formula was designed to target latent viruses. Therefore, the theory predicts that treatment with Gene-Eden-VIR will decrease fatigue in individuals infected with a latent virus. The objective of this study was to test this prediction. Framework: A post marketing clinical study that followed FDA guidelines. Treatment: Gene-Eden-VIR, a dietary supplement. A capsule of Gene-Eden-VIR includes 100 mg of quercetin, 150 mg of green tea extract, 50 mg of cinnamon extract, 25 mg of licorice extract, and 100 mcg of selenium. The treatment included 1, 2, 3, or 4 capsules per day, and lasted 2 to 54 weeks. Population: 100 individuals infected with a latent virus, including the Human Papillomavirus (HPV), Epstein Barr Virus (EBV), Herpes Simplex Virus (HSV), Human Cytomegalovirus (HCMV), and Hepatitis C Virus (HCV). Ages ranged from 20 to 66. All participants reported a feeling of fatigue at the start of the study. Specifically, 98, 90, and 79 participants reported a feeling of general, physical, and mental fatigue, respectively. Results: Following treatment with Gene-Eden-VIR, 73.47%, 62.22%, and 47.36% reported a decrease in their feeling of general, physical, and mental fatigue, respectively. The participants also reported a statistically significant decrease in every aspect of fatigue tested in the study. The results also showed a duration effect, that is, those treated for 2 months or more reported a larger decrease in their feeling of fatigue (general, p = 0.03, n = 65; physical and mental, p = 0.05, n = 70). The results showed no interviewer bias, and no selection bias. In addition, the results showed therapeutic consistency under varying manufacturing conditions. The participants reported no side effects after taking Gene-Eden-VIR. Conclusions: This post marketing clinical study showed that treatment with Gene-Eden-VIR safely decreased the feeling of general, physical, and mental fatigue in individuals infected with a latent virus. Since most individuals are infected with a latent virus, health care practitioners should recommend Gene-Eden-VIR as a first line treatment for fatigue. The results of this post marketing clinical study support the Microcompetition with Foreign DNA theory.

Effect of Green Tea Extract on the Interferon-Induced Testicular Apoptosis in the Adult Albino Rat: Immunohistochemical and Electron Microscopic Study

Background: Interferons (IFNs) are widely used in the treatment of various diseases and can disrupt spermatogenesis and inhibition Leydig cell steroidogenesis through the formation of reactive oxygen species (ROSs) that cause oxidative stress. Aim of the work: In this study we examine the effect of (IFN) on rat spermatogonia and investigate the possible protective effect of Grean tea on (IFN)-induced spermatogonia appotosis. Material and Methods: Forty-eight adult male albino rats (240-280 g) were used in this study. They were divided into three groups. Group I in which rats were served as a control and were given sterile normal saline. The rats in Group II were given 7.500 units of interferon (INF) α-2β, considered in clinical treatment dose range, 3 times weekly under inhalation anesthesia for 2 month (Group II a) and 3 month (Group II b). Group III received Green tea extract and interferon α-2β in the same dose of group (II) for 2 month (Group III a) and 3 month (Group III b). 200 mg of green tea extract was dissolved in distal water and solution was administrated orally through gastric tube at dose level of (40 mg/k.g.b.w.). At the end of the 2 and 3 month respectively, bilateral orchiectomy was performed through standard ilioinguinal incisions and the rats were sacrified by pentobarbital overdose (200 mg=kg) inhalation. The testes were removed and separated. A part of one testis was fixed in Bouin fixative to be used for histopathological and immunohistochemical studies for p53 and the other part was used in a fresh state to determine the level of the Superoxide Dismutase (SOD). The other testis was fixed in 1% gluteraldehyde and 4% formaldehyde in (pH 7.4) for at least 2 hours to overnight. Ultrathin sections processed for transmission electron microscopic studies. Results: Interferon induced testicular damage that is revealed by loss of testicular architecture through Hx. and E. stained sections, Immunohistochemical stained sections against p53 and the electron microscopic examination. These findings were accompanied by significant reduction in diameter of seminiferous tubules and thickness of the germinal epithelium with significant reduction in SOD. Otherwise, the green tea extract definitely reverse the above mentioned findings with improvement of testicular architecture and significant increase in diameter of seminiferous tubules, thickness of the germinal epithelium and elevated SOD. The mean of the diameter of the seminiferous tubules of Group IIa showed a significant decrease by nearly 34% in comparison to Group IIb showed a higher significant decrease by nearly 43%. The mean of the thickness of the seminiferous tubules of Group IIa showed a significant reduction by nearly 33% while in Group IIb showed a higher significant reduction by nearly 50%. The mean of the level of the Superoxide Dismutase in Group IIa showed significant decrease by nearly the half while in Group IIb revealed a higher significant decrease by nearly 59%. The mean of the diameter of the seminiferous tubules in Group IIIa showed a significant increase by nearly 32% and by nearly 43% in Group IIIb. The mean of the thickness of the seminiferous tubules in Group IIIa showed significant increase by nearly 11% while in Group IIIb the significant increase was by nearly 45%. The mean of the level of the Superoxide Dismutase of the seminiferous tubules in Group IIIa did not show significant increase while in Group IIIb revealed a higher significant increase by nearly 48% Group IIIb. Conclusion: The green tea extract has a powerful antioxidant effect that can reverse the damage caused

Abstract 525: Evaluation the Effect of Green Tea on Blood Pressure, Endothelial Function, Metabolic Profile, Inflammatory Activity and Body Adiposity in Obese Pre-hypertensive Women

Background: Cardiovascular diseases are the leading cause of mortality in Western countries. Some studies have suggested that green tea has beneficial effects on different cardiovascular risk factors. However, others have failed to show such an association. Objective: To evaluate the effects of green tea on blood pressure, endothelial function, metabolic profile, inflammatory biomarkers and body adiposity in obese pre-hypertensive women. Methods: Crossover randomized controlled double-blinded trial. Twenty women with obesity and pre-hypertension, aged 28-59 years, with stable body weight were randomized to receive a daily supplement of 3 capsules that contained either 500mg of green tea extract (GTE) or a matching placebo for 4 weeks, with a washout period of 2 weeks between the treatments. Blood pressure was evaluated by ambulatory blood pressure monitoring (ABMP). Endothelial function was evaluated by peripheral arterial tonometry method, using Endo-PAT 2000®. The inflammatory status was assessed by interleukin 6, tumor necrosis factor alpha, vascular cell adhesion molecule, intercellular adhesion molecule, plasminogen activator inhibitor-1, vascular endothelial growth factor, E-selectin, adiponectin and C-reactive protein. Results: After administration of GTE compared with placebo there was a significant reduction in systolic blood pressure during 24h (pre 130.3±1.7 vs. post 127.0±2.0 mmHg; p= 0.02), daytime (pre 134.0±1.7 vs. post 130.7±2.0 mmHg; p= 0.04) and nighttime (pre 122.2±1.8 vs. post 118.4±2.2 mmHg; p= 0.02). After consumption of green tea, there was an increase, although not statistically significant, in reactive hyperemia index (pre 1.98±0.10 vs. post 2.22±0.14), besides reduction in the concentration of intercellular adhesion molecule (pre 91.8±8.0 vs. post 85.8±5.6 ng/ml) and vascular endothelial growth factor (pre 195.8±46.2 vs. post 158.6±38.7 pg/ml), however without statistical significance. The other variables did not change significantly after consumption of green tea. Conclusions: The findings of the present study suggest that green tea has a beneficial effect on blood pressure and possibly on endothelial function.

Treatment of symptomatic uterine fibroids with green tea extract: a pilot randomized controlled clinical study

BackgroundUterine fibroids (UFs, also known as leiomyoma) affect 70% of reproductive-age women. Imposing a major burden on health-related quality-of-life (HRQL) of premenopausal women, UF is a public health concern. There are no effective medicinal treatment options currently available for women with symptomatic UF.ObjectivesTo evaluate the efficacy and safety of green tea extract (epigallocatechin gallate [EGCG]) on UF burden and quality of life in women with symptomatic UF, in a double-blinded, placebo-controlled randomized clinical trial.MethodsA total of 39 reproductive-age women (age 18–50 years, day 3 serum follicle-stimulating hormone <10 \\U/mL) with symptomatic UF were recruited for this study. All subjects had at least one fibroid lesion 2 cm3 or larger, as confirmed by transvaginal ultrasonography. The subjects were randomized to oral daily treatment with either 800 mg of green tea extract (45% EGCG) or placebo (800 mg of brown rice) for 4 months, and UF volumes were measured at the end, also by transvaginal ultrasonography. The fibroid-specific symptom severity and HRQL of these UF patients were scored at each monthly visit, using the symptom severity and quality-of-life questionnaires. Student’s t-test was used to evaluate statistical significance of treatment effect between the two groups.ResultsOf the final 39 women recruited for the study, 33 were compliant and completed all five visits of the study. In the placebo group (n = 11), fibroid volume increased (24.3%) over the study period; however, patients randomized to green tea extract (n = 22, 800 mg/day) treatment showed significant reduction (32.6%, P = 0.0001) in total UF volume. In addition, EGCG treatment significantly reduced fibroid-specific symptom severity (32.4%, P = 0.0001) and induced significant improvement in HRQL (18.53%, P = 0.01) compared to the placebo group. Anemia also significantly improved by 0.7 g/dL (P = 0.02) in the EGCG treatment group, while average blood loss significantly decreased from 71 mL/month to 45 mL/month (P = 0.001). No adverse effects, endometrial hyperplasia, or other endometrial pathology were observed in either group.ConclusionEGCG shows promise as a safe and effective therapeutic agent for women with symptomatic UFs. Such a simple, inexpensive, and orally administered therapy can improve women’s health globally.

Gene-Eden-VIR Is Antiviral: Results of a Post Marketing Clinical Study

Introduction: This paper reports the results of a post marketing clinical study that tested the antiviral properties of Gene-Eden-VIRTM. Specifically, the clinical study tested the effect of Gene-Eden-VIR on the severity, duration, and frequency of symptoms reported by individuals infected with various viruses. The viruses included the Human Papillomavirus (HPV), Herpes Simplex Virus (HSV), Epstein Barr Virus (EBV), Human Cytomegalovirus (HCMV) and Hepatitis C Virus (HCV). The symptoms included abnormal Pap smear, low and high grade cervical dysplasia, warts, blisters, cold sores, hives, skin tabs, panic attacks, depression, kidney problems, sleeping problems, liver problems, fever, fatigue, sore throat, swollen lymph nodes, diarrhea, and weight loss. Treatment: A capsule of Gene-Eden-VIR includes five natural ingredients: 100 mg of quercetin, 150 mg of green tea extract, 50 mg of a cinnamon extract, 25 mg of a licorice extract, and 100 mcg of selenium. The dosage was 1, 2, 3, or 4 capsules per day. The duration of treatment was 2 to 54 weeks. Population: The study population consisted of 60 infected individuals, ages 20 to 66. Results: The participants reported no side effects after taking Gene-Eden-VIR. Seventy three percent of the individuals treated with Gene-Eden-VIR reported a decrease in their symptoms. Specifically, they reported a decrease in the severity (p = 0.006, n = 45), duration (p = 0.009, n = 34), and frequency of their symptoms (p Conclusions: This post marketing clinical study showed that Gene-Eden-VIR is a safe and effective antiviral treatment. Specifically, the clinical study showed that Gene-Eden-VIR is a safe and effective treatment against the Human Papillomavirus (HPV), Herpes Simplex Virus (HSV), Epstein Barr Virus (EBV), Human Cytomegalovirus (HCMV), and Hepatitis C Virus (HCV). Therefore, health care practitioners should recommend Gene-Eden-VIR as a safe and effective antiviral treatment against these viruses.

Effects of polyphenol oxidation onin vitroiron availability in faba bean (Vicia fabaL.) flour

Tannic acid (TA) and green tea extract (GTE) were added to faba bean flour, before and after incubation with polyphenol oxidase (mushroom tyrosinase), and the effect on in vitro iron availability was investigated. The inhibitory effect of TA and GTE was dose dependent, and the in vitro iron availability decreased from 64.5% in the pure faba bean flour to 9.8% (P < 0.05) with the addition of 1 mg of TA 1 g−1 to faba bean flour. The addition of 5 mg of GTE resulted in low in vitro iron availability as after the addition of 10 mg of TA (11.6% against 10.3% for 1 mg TA). Incubation of the polyphenols with tyrosinase before addition to the faba bean flour significantly increased the in vitro iron availability. The increase in iron availability was significant (P < 0.05) when amounts of 0.2, 0.5 or 1 mg g−1 TA or GTE added to faba bean flour, respectively, were oxidised, even with the lowest amount of tyrosinase (150 u). Oxidation of 1 mg TA or GTE with 150 u tyrosinase increased the in vitro iron availability from 10.3% to 15.5% and from 19.2% to 26.1%, respectively. At the 300-u level, the addition of higher amounts of enzyme (from 600 to 900 u) did not have any effect. The results from the study therefore suggest that the oxidation of polyphenols may be a promising way to increase the availability of iron in polyphenol-containing legume foods.

Green tea extract reduces blood pressure, inflammatory biomarkers, and oxidative stress and improves parameters associated with insulin resistance in obese, hypertensive patients

Green tea (GT) consumption is known to be associated with enhanced cardiovascular and metabolic health. The purpose of this study is to examine the hypothesis that supplementation with GT alters insulin resistance and associated cardiovascular risk factors in obese, hypertensive patients. In a double-blind, placebo-controlled trial, 56 obese, hypertensive subjects were randomized to receive a daily supplement of 1 capsule that contained either 379 mg of GT extract (GTE) or a matching placebo, for 3 months. At baseline and after 3 months of treatment, the anthropometric parameters, blood pressure, plasma lipid levels, glucose levels, creatinine levels, tumor necrosis factor α levels, C-reactive protein levels, total antioxidant status, and insulin levels were assessed. Insulin resistance was evaluated according to the homeostasis model assessment-insulin resistance protocol. After 3 months of supplementation, both systolic and diastolic blood pressures had significantly decreased in the GTE group as compared with the placebo group (P < .01). Considerable (P < .01) reductions in fasting serum glucose and insulin levels and insulin resistance were observed in the GTE group when compared with the placebo group. Serum tumor necrosis factor α and C-reactive protein were significantly lower, whereas total antioxidant status increased in the GTE group compared with the placebo (P < .05). Supplementation also contributed to significant (P < .05) decreases in the total and low-density lipoprotein cholesterol and triglycerides, but an increase in high-density lipoprotein cholesterol. In conclusion, daily supplementation with 379 mg of GTE favorably influences blood pressure, insulin resistance, inflammation and oxidative stress, and lipid profile in patients with obesity-related hypertension.

Effects of Green Tea Supplementation on Elements, Total Antioxidants, Lipids, and Glucose Values in the Serum of Obese Patients

The consumption of green tea has been associated with cardiovascular and metabolic diseases. There have been some studies on the influence of green tea on the mineral status of obese subjects, but they have not yielded conclusive results. The aim of the present study is to examine the effects of green tea extract on the mineral, body mass, lipid profile, glucose, and antioxidant status of obese patients. A randomized, double-blind, placebo-controlled study was conducted. Forty-six obese patients were randomly assigned to receive either 379 mg of green tea extract, or a placebo, daily for 3 months. At baseline, and after 3 months of treatment, the anthropometric parameters, blood pressure, and total antioxidant status were assessed, as were the levels of plasma lipids, glucose, calcium, magnesium, iron, zinc, and copper. We found that 3 months of green tea extract supplementation resulted in decreases in body mass index, waist circumference, and levels of total cholesterol, low-density cholesterol, and triglyceride. Increases in total antioxidant level and in zinc concentration in serum were also observed. Glucose and iron levels were lower in the green tea extract group than in the control, although HDL-cholesterol and magnesium were higher in the green tea extract group than in the placebo group. At baseline, a positive correlation was found between calcium and body mass index, as was a negative correlation between copper and triglycerides. After 3 months, a positive correlation between iron and body mass index and between magnesium and HDL-cholesterol, as well as a negative correlation between magnesium and glucose, were observed. The present findings demonstrate that green tea influences the body's mineral status. Moreover, the results of this study confirm the beneficial effects of green tea extract supplementation on body mass index, lipid profile, and total antioxidant status in patients with obesity.

Impact of Bifidobacterium animalis subsp. lactis BB-12 and, Lactobacillus acidophilus LA-5-containing yoghurt, on fecal bacterial counts of healthy adults

This randomized, placebo-controlled, double blind, parallel dose-response study investigated the impact of 4-week commercial yoghurt consumption supplemented with Bifidobacterium animalis subsp. lactis (BB-12) and Lactobacillus acidophilus (LA-5) on fecal bacterial counts of healthy adults. Fifty-eight volunteers were randomly assigned to three different groups: 1. placebo (no probiotic, no starter and no green tea extract); 2. Yoptimal (10(9)cfu/100g of BB-12 and LA-5 and 40mg of green tea extract) and 3. Yoptimal-10 (10(10)cfu/100g of BB-12, 10(9)cfu/100g of LA-5 and 40mg of green tea extract). These yoghurt products also contained Lactobacillus delbrueckii subsp. bulgaricus (10(7)cfu/100g) and Streptococcus thermophilus (10(10)cfu/100g). The quantitative PCR (qPCR) results showed that there were significant increases (P=0.02) in bifidobacteria counts with the Yoptimal treatment as compared to baseline. The fecal numbers of B. animalis subsp. lactis and LA-5 significantly increased in the two probiotic treatments compared to the placebo treatment. Viable counts of fecal lactobacilli were significantly higher (P=0.05) and those of enterococci were significantly lower (P=0.04) after the intervention when compared to placebo. No significant difference was observed between treatments in volunteers' weight, waist girth, blood pressure, fasting plasma triglyceride and HDL-C concentrations, as well as cholesterol/HDL-cholesterol ratio. However, a significant increase in plasma cholesterol levels was observed in the placebo group (P=0.0018) but the levels remained stable in the two probiotic yoghurt groups. These results show that probiotic strains supplemented in the form of yoghurt remain active during gut transit and are associated with an increase in beneficial bacteria and a reduction in potentially pathogenic bacteria. This trial was registered at clinicaltrials.gov as NCT00730626.

Possible green tea-induced thrombotic thrombocytopenic purpura

A case of a patient who developed thrombotic thrombocytopenic purpura (TTP) after consuming a weight-loss product containing green tea is reported. A 38-year-old, 68-kg Caucasian woman arrived at the emergency department with a one-week history of malaise, fatigue, and petechiae of the skin. She had no symptoms of infection and denied illegal drug use. Her medical history included hypothyroidism, for which she was treated with levothyroxine 150 microg daily for the past four years. She reported that she had been using a green tea preparation for the two months before admission to lose body weight. The daily preparation contained 200 mg of green tea extract 5:1, equivalent to 1 g of natural green tea. On clinical examination, the patient appeared acutely ill and was afebrile, with pallor, petechiae, and purpura of the extremities. Laboratory test results at the time of admission revealed that the patient had anemia and marked thrombocytopenia. A peripheral blood smear demonstrated a feature of microangiopathic hemolytic anemia. Immunoglobulin G autoantibodies against ADAM metallopeptidase with thrombospondin type 1 motif, 13 were detected. On hospital day 3, the patient appeared confused and exhibited aphasia that was initially transient but then recurrent. Brain computerized tomography did not exhibit focal pathology. Over the next few days, her neurologic symptoms subsided and her platelet count and hematocrit value gradually increased. Plasmapheresis was performed (12 procedures). Corticosteroid treatment was also initiated. After 20 days of hospitalization, the patient was discharged. A 38-year-old woman developed TTP after consuming a weight-loss product containing green tea extract for two months.