Methylene Blue Test Research Articles

Effect of temperature on the physical, optical and photocatalytic properties of TiO2 nanoparticles

In this paper we describe the effect of calcination temperature on the structural, morphological, optical and photocatalytic properties of TiO2 nanoparticles. The titanium dioxide powders were produced by the sol–gel method starting from titanium tetraisopropoxide (TTIP) in neutral aqueous medium (pH 5.5). After that, the TiO2 nanoparticles were treated at three different calcination temperatures for 4 h: 100, 450 and 800 °C. Then, the powders were characterized by XRD, Raman and photoluminescence techniques. We observed the anatase phase for the powders treated at 100 and 450 °C and rutile for that treated at 800 °C. The photocatalytic activity of the TiO2 powders was investigated using Methylene Blue test and showed a strong correlation with the temperature (i.e. TiO2 phase). The better photocatalysis exhibited by the nanoparticles treated at 100 and 450 °C compared to the powder at 800 °C was due to the higher recombination of photo-generated electrons and holes of rutile with respect to anatase.

Study on Physico-Chemical and Microbial Quality of Raw Milk Collected From Different Places of Assi Region in Varanasi City, Varanasi

The present study was conducted to evaluate the physico-chemical characteristics including Adulteration and Microbiological quality of cow raw milk collected from four different places of ASSI region in Varanasi. Samples were analysed to know the chemical composition, the results showed that the statistically average percentage of Moisture (87.46), Fat (3.87), Protein (3.15), Lactose (4.42), Ash (.712), pH (6.43) and acidity (0.147). The keeping quality of milk was evaluated by Methylene Blue Reduction Test (MBRT). This phenomenon testified that milk sample 1 is fair quality and remained sample were found good and excellent. The microbiological conclusion confirmed the presence (less or more) of microbial load in all the raw milk samples. The highest level of microbial quality in standard plate count (SPC) was 19.1×106 cfu/ml. in sample 1 and in logarithm value is 7.28 cfu/ml at the same time, the highest coliform bacteria 2.3×102 in logarithm value is 2.36 was found in the sample 2. The adulterations in raw milk were checked by the standard procedure. In cow’s raw milk the different mixed adulterant were found in two samples contaminated with detergent and pulverized soap. Besides different hazardous chemical adulterant, raw milk from sample 1 was detected with presence of hydrogen peroxide and sample 2 was contaminated formalin whereas urea was present in sample 2 and 4. Milk adulteration is a global concern and social problem. Increased demand, growth in competition in dairy industry and financial gain makes some producers to adulterate the milk thereby decreasing milk quality.

DIAGNOSTICS OF UNFIRED EARTH WITH METHYLENE BLUE TEST

Unfired earth is important historic building material. Final properties of unfired earth constructions are influenced by amount and kind of clay in earth mixtures. Methylene blue test is a method that helps determining the type and amount of clay in earth mixtures. This paper describes research focused on using methylene blue test for analysis of methylene blue dye absorption by montmorillonite clay. The first part of the paper describes principle of methylene blue test and course of experimental measurement. The second part deals with analysis of obtained results. On the basis of experimental measurement it can be stated that methylene blue test is influenced by the stirring time of earth samples with methylene blue solution and dependence between amount of methylene blue dye absorption and amount of montmorillonite clay in earth samples is linear.

The extended methylene blue reduction test and milk quality

Introduction. This study aimed to evaluate the quality of milk produced by six cattlemen’s associations in small, isolated farming communities of Carchi, Ecuador. It involved a herd of 814 cows and lasted eight consecutive months. Another aim was to propose a suitable methodology for milk quality evaluation.
 Study objects and methods. All milk samples were analyzed for total solids, protein, fat, acidity, density, total bacterial count (TBC) and somatic cell count (SCC). Each sample was subjected to an extended qualitative methylene blue reduction test (MBRTe) for which 10 mL of milk, with 0.5 mL of methylene blue, was incubated at 37°C for 24 h. 
 Results and discussion. As a result, we obtained the following types of clots: MBRTe-I (homogeneous solid/liquid clot), MBRTe-II (lumpy clot), MBRTe-III (gaseous clot) and MBRTe-IV (lumpy + gaseous clot). The study showed significant differences in the quality of milk between different associations, suggesting that some of them did not comply with good practices of milking, handling and storage of fresh milk. The quality of milk was classified as good in one association, as regular in another association, and as low in four associations. The MBRTe classified 37% of the samples as MBRTe-I, 18% as MBRTe-II, 14% as MBRTe-III and 12% as MBRTe-IV. Of the MBRTe-I samples, 95% showed the TBC and SCC values of first quality milk. The MBRTe-II had the TBC values of first quality milk, but exceeded the SCC, while the MBRTe-III had good SCC values, but exceeded the TBC. Finally, the MBRTe-IV samples exceeded the permissible levels of both TBC and SCC.
 Conclusion. It was proved that the MBRTe can help milk producers evaluate the quality of milk and alert them to the possible presence of mastitis in the herd. The MBRTe is a reliable and cheap method that is quick and easy to perform.

Vesicovaginal fistula following pelvic surgery: Our experiences and recommendations for diagnosis and prompt referral.

Vesicovaginal fistula (VVF) is an uncommon but known complication of pelvic surgery. Post-operative urine leakage should generate a high index of clinical suspicion for early diagnosis of VVF. This study aims to identify areas for improvement and provide recommendations for timely and accurate diagnosis of VVF to minimise patient morbidity. A retrospective observational study of all patients who underwent VVF repairs unrelated to malignancies in a ten-year period at two primary referral centres in Brisbane was performed to review the aetiology, performance of investigative tests and management of these cases. A total of 56 patients were studied with the identification of significant delays to diagnosis and referral, the performance of multiple unnecessary tests and low rates of primary surgeon referral. Early recognition of VVF and assessment by outpatient methylene blue dye test can provide quick same-day diagnosis and minimise patient suffering. We hope this will alert gynaecologists and provide guidelines for diagnosis and improved management to allow for prompt referral and repair of genitourinary fistula.

Green Reduction of Graphene Oxide using Kaffir Lime Peel Extract (Citrus hystrix) and Its Application as Adsorbent for Methylene Blue

Green reduction of graphene oxide (GO) by phytochemicals was explored using the aqueous extract of kaffir lime peels. The research methods included preparation of extracts, preparation of GO, preparation and characterization of reduced-GO (RGO) using Fourier Transform Infrared (FTIR), X-ray diffraction (XRD), and UV-Vis spectroscopy, as well as methylene blue (MB) adsorption test using RGO. The RGO characterization showed that GO was successfully reduced by a C=C group restoration. The MB adsorption kinetics profile in RGO is more suitable for the pseudo-second-order model, whereas for the adsorption isotherm it is more suitable for the Langmuir model with a maximum adsorption capacity (qmax) of 276.06 mg/g at room temperature. The best ratio of GO: kaffir lime peel extract used to prepare RGO was at a ratio of 1: 2. Based on the ΔG, ΔH, and ΔS values, the adsorption of RGO-MB was defined as spontaneous and endothermic process. The results promise the potential application of RGO derived via green route to remove cationic dye in wastewater.

Role of Laparoscopic Surgery in the Management of Female Infertility at the Department of Gynecology of Hôpital du Mali

Infertility is a socio-cultural drama in Africa, especially in Mali and remains difficult for couples to overcome. Laparoscopy, also called minimally invasive surgery or keyhole surgery, is an operative technique that permits to explore the pelvis and perform an appropriate therapeutic procedure. The objective of our work was to assess the role of laparoscopic surgery in the treatment of female infertility at Hopital du Mali. It was a retrospective descriptive study that was conducted over a period of 5 years (January 2013 to December 2018). Any patients followed for infertility and who underwent laparoscopic surgery in the gynecology department over a period of two (2) years were included. The outcome of laparoscopic surgery was evaluated in terms of conception of pregnancy. We had collected 103 infertile patients out of 2984, with a frequency of 3.45%. The mean age of our patients was 30.2 years. Housewives and out-of-school women accounted for 68.93% and 54.37% respectively. Nulligravida represented 41.75%. Regarding infertility, it was primary in 41.75% and secondary in 58.25%, with an average duration of 6.2 years. Systematic chlamydial serology was positive in 62.14% of our patients. Hysterosalpingography (HSG) revealed bilateral tubal obstruction in 53.33% of cases. Laparoscopy found tubal adhesions in 97.80% of cases. Adhesiolysis was the main procedure performed with 84.47% bilateral tubal patency achieved in the methylene blue test. Among our patients who had contracted a pregnancy (22.99%), 85% had carried their pregnancies to term. Tubal damage was the main cause of infertility. Adhesiolysis remains the main laparoscopic procedure for treatment.

PH-sensitive Pickering Emulsion Stabilized by Hydroxyapatite Nanoparticles: Stability and Controlled Release Study

This study investigates the formulation of surfactant-free Pickering emulsions that release a drug at a specific pH to improve its oral bioavailability. The stabilizing nanoparticles composed of hydroxyapatite were obtained by a process of nanoprecipitation. Pickering oil-in-water emulsions stabilized with hydroxyapatite nanoparticles and encapsulating a hydrophobic drug model (ibuprofen) were formulated using a high-energy process with rotor-stator turbo mixer (IKA® T25 digital ultra-Turrax). The experimental approach explored the impact of all formulation parameters, dispersed phase and amount of hydroxyapatite nanoparticles on the physicochemical properties of Pickering emulsions. The system was characterized by a methylene blue test, pH and conductivity measurements, and droplet size determination. In addition, Pickering emulsions stabilized by hydroxyapatite nanoparticles have the advantage of being destabilized in acidic medium leading to the release of the active principle via the droplets. The acidic medium release study (pH equal to 1.2) showed ibuprofen release as a function of initial droplet loading and saturation concentration. In the simulated intestinal medium at pH equal to 6.8, we found a better release of ibuprofen from emulsions that already had saturation in an acid medium. Thus, the interest of these Pickering emulsions lies on the fact that their non-toxicity and hydroxyapatite nanoparticles have advantage of being biocompatible because having the same mineral composition as bones and teeth. In addition, they allow destabilization of the emulsions and release of the drug. These emulsions not only protect patients from the side effects of acid-based drugs, but also contribute to increase the bioavailability of these acidic drugs.

Antioxidant activity of polymeric biocide polyhexamethylene guanidine hydrochloride

Cationic polymer polyhexamethylene guanidine hydrochloride (PHMG-Cl) is promising biocide that combines a broad spectrum of antimicrobial activity, mod-erate toxicity, as well as reasonable cost. It is widely used as an effective disinfect-ant in cooling systems, swimming pools, and hospitals, personal hygiene products, etc. Recently PHMG-Cl was found to have pronounced anti-inflammatory and wound healing properties and therefore may be used for the treatment of chronic wounds and thermal burns. This may indicate the antioxidant activity of polymeric biocide. In this study, PHMG-Cl has been synthesized by melt polycondensation of guanidine hydrochloride and 1,6-hexamethylenediamine. The structure of the cati-onic polymer was confirmed by 1H NMR and IR spectroscopy. The viscosity-average molecular weight of PHMG-Cl was found to be 10700. The antioxidant activity of PHMG-Cl has been studied by using different methods. In the methylene blue (MB) dye test, the oxidation of MB by hydroxyl radicals generating in Fenton’s system was found to decrease in the presence of PHMG-Cl in a molar ratio to MB of 5:1 and 10:1 (by 26 % and 38 %, respectively). At the same time, complete dye oxidation was observed when guanidine hydrochloride was used instead of PHMG-Cl. The antioxidant activity of PHMG-Cl has also been studied in the model system of radical chain oxidation of benzyl alcohol (BA). In this system, alkyl and peroxyl radicals are formed. The antioxidant activity was determined by a decrease of the initial rate of oxygen absorption during the initiated oxidation of BA. The introduc-tion of PHMG-Cl into the oxidized system in the concentrations ranged from 1.3·10-3 - 1.6·10-2 mol/l decreased the oxidation rate of BA by 4.5–88 %. This result demonstrates that PHMG-Cl effectively inhibits radical chain oxidation of BA. However, further research is needed to elucidate the mechanism of free radical de-activation by a polymer biocide.

Influence of pre and postpartum alpha-tocopherol supplementation on milk yield, milk quality, and udder health of Jersey crossbred cows at tropical lower Gangetic region.

Background and Aim:Alpha-tocopherol supplementation influences milk yield, milk quality, and udder health of dairy cows, which needs to be investigated for Jersey crossbred cows at hot-humid climate. Therefore, the present study was framed with an objective to study the effect of pre and postpartum Alpha-tocopherol supplementation on milk yield, milk quality, and udder health status of Jersey crossbred cows at tropical climate.Materials and Methods:For this study, 19 similar parity, body condition score, and production level dairy animals were separated randomly into three groups, namely, Control, T1 and T2. Control group (no supplementation) was compared with two treatment groups, namely, T1 and T2. Supplementation of Alpha-tocopherol was done in concentrate fed to the animals (at 1 g/cow/day) 30 days prepartum to 30 days postpartum in T1 and 30 days prepartum to 60 days postpartum in T2 groups. Observations were taken for different parameters up to 5 months of lactation.Results:Statistically analyzed data revealed that overall significantly (p<0.01) more milk production was found in T2, followed by T1 than the control group. Overall significantly (p<0.01) lower somatic cell counts and modified California mastitis tests were recorded in T2, followed by T1 than the control group. Overall significantly (p<0.01), better milk quality in terms of methylene blue reduction test was found in T2, followed by T1 than control groups. Differences in the milk composition of all three groups were non-significant (p>0.05).Conclusion:Supplementation of Alpha-tocopherol during prepartum to initial lactation period may enhance milk yield, milk quality, and udder health status of Jersey crossbred cows at the tropical lower Gangetic region.

Medical Management of Rumen Collapse in Cows

Rumen collapse or rumen void is a syndrome usually observed in cattle affected with other primary diseases. It is a sequelae to other primary diseases due to total anorexia for several days. Recently purchased and calved animals brought into Large Animal Outpatient Medicine Unit, Madras Veterinary College Teaching Hospital, with the history of prolonged anorexia, mastitis and metritis were screened for rumen collapse. A total of 20 cases were included for the study. Animals were subjected to clinical, physical, rectal, haematological and biochemical examination. Anamnesis included were anorexia in all cases, high rise of temperature 15(75%) and high coloured urine 14(70%). An rumen collapsed in case of recently purchased animals were 12(60%). Clinical signs were anemia 18(90%), enlarged lymph node 17(85%), decreased rumen motility 17(85%), decreased or suspended rumination 16(80%). Rectal examination revealed a small sized rumen (rumen collapsed). Rumen fluid examination revealedvery few live protozoa or complete absence of protozoal motility and increased in MBRT (Methylene blue reduction test) time. The haematological examination revealed reduced level of Hb, PCV and RBC level. Biochemical examination revealed reduced level of total protein and other parameters were remain in the normal range. The suggestive of the primary diseases involved were hypocalcaemia, theileriosis, anaplasmosis, mastitis, septic metritis, indigestion and babesiosis. The animals were firstly treated for primary diseases followed by rumen cud transplantation and oral administration of rumenotorics bolus.

A Decalogue to Avoid Routine Ileostomy in Selected Patients With Border Line Risk to Develop Anastomotic Leakage After Minimally Invasive Low-Anterior Resection: A Pilot Study.

Purpose. Protective ileostomy (PI) during anterior resection (AR) for rectal cancer decreases the incidence of anastomotic leakage (AL) and its subsequent complications, but it may itself be the cause of morbidity. The aim is to report our protocol in the management of selected patients with borderline risk to develop AL after laparoscopic AR and ghost ileostomy (GI) creation. Methods. Patients who underwent AR were stratified based on the risk to develop AL. Steps to avoid PI were splenic flexure mobilization, reduced pelvic bleeding, to employ different stapler charge if neoadjuvant chemo-radiotherapy is performed, to perform a horizontal section of the rectum, to evaluate the anastomotic vascularization with a fluorescence angiography, to perform a side-to-end anastomosis, intraoperative methylene blue test, pelvic and transanal drainage tubes placement, and the GI creation. After surgery, inflammatory blood markers were monitored to detect potential leakages. Results. Twelve patients were included. In one case, the specimen proximal section was changed after fluorescence angiography. There were no conversions in this group of patients. One postoperative AL occurred and was treated with radiological drainage placement, not being necessary to convert the GI. PI was avoided in 100% of cases. Conclusions. Patients' characteristics cannot be changed, but several steps were used to avoid routine PI creation. The present protocol could be a valuable option to avoid PI in selected patients. Further studies with a wider sample size, and defined criteria to stratify the patients based on the risk to develop AL, are required.

Analysis of Strength Development and Soil-Water Characteristics of Rice Husk Ash-Lime Stabilized Soft Soil.

With increased awareness of environmental protection, the output of traditional curing agents such as cement and lime is less and less, so it is urgent to develop new curing agents with high efficiency and environmental benefits. Thus, this study aims at investigating the application of rice husk ash (RHA) from agricultural waste to the soft soil stabilization. A series of tests are conducted to analyze the strength development process and soil–water characteristics of rice husk ash–lime (RHA–lime) stabilized soils. The results of the strength tests showed that by increasing the content of RHA, the unconfined compressive strength (UCS) and splitting strength of stabilized soils increased first and then decreased. The effective shear strength indexes of the three soil types (soft soil, lime-stabilized soil, and RHA–lime soil) are measured and compared. It is found that RHA can effectively improve the shear resistance and water resistance of stabilized soil. The results of methylene blue test demonstrated that RHA can also promote the reduction of the specific surface area and swelling potential energy of lime-stabilized soil. In addition, the influence of RHA on mineral composition and morphology change in stabilized soils is studied at the microscopic level. The X-ray diffraction tests and scanning electron microscope (SEM) tests showed that strength development and change of soil–water properties of RHA–lime stabilized soil are attributed to enhanced cohesion by cementation and pores filling with agglomerated mineral.

TiO2 based nanopowder coatings over stainless steel plates for UV‐C photocatalytic degradation of methylene blue

AbstractImmobilizing the photocatalyst in a water treatment process design is essential; however, the immobilization method may affect the photoactivity of the photocatalyst. In this work, photocatalyst powders were successfully coated on 316 L stainless steel plates by a novel brush coating method. Three combinations of photocatalyst mixtures (pure TiO2 anatase, anatase doped with WO3, or TiO2 rutile) were and annealed at different temperatures between 460‐540°C. The ~ 10 μm thick coatings demonstrated full plate coverage and strong adhesion and adequate durability. Surface roughness increased with annealing temperature. The doping and annealing process enabled band gap reduction to the visible light spectrum for all coatings, with the smallest band gap being 2.48 eV (1 eV = 1.6 × 10−19 J). Subsequent methylene blue degradation tests under UV‐C showed that the coatings annealed in 460°C exhibited the best performance and with the highest degradation rate constant of 5.59 hours−1.

Effect of varying deposition conditions of magnetite on sawdust on the physiochemical properties of the prepared composites

This work reports a novel study for deposition of magnetite (Mag) on olive wood sawdust (OW) by the coprecipitation-hydrothermal method under dominating deposition factors including deposition temperature of magnetite by OW, agitation time, magnetite deposition in presence/absence of OW, magnetite/OW mass ratio, pyrolysis of final composites. The composites were characterized by IR, XRD, TGA, surface area (by N2 and methylene blue adsorption test) and lead uptake The effect of experimental factors on physicochemical properties were addressed. Principal component analysis (PCA) was found effective for understanding the differences among composites. It was found that magnetite deposition has relatively increased the stability of OW against degradation. XRD and IR data showed that not only various deposition conditions caused deposition of various amounts of iron oxides and magnetite on wood surface; but also the amount of deposited iron oxides exceeded that of magnetite. Pb ions adsorption was significantly affected by the deposition conditions in which deposition time was the most dominant among the tested factors as 5 h stirring (at 50 °C) achieved the best lead uptake. In general, deposition of magnetite by OW reduced porosity of composites. The prepared composites were effective for Pb removal with capacitates 22–49 mg g−1 at pH 7.0. Not all physicochemical parameters were effective for composites grouping. Surface areas and Pb uptake were most responsive for experimental conditions.

3D Printing of Mixed Matrix Films Based on Metal-Organic Frameworks and Thermoplastic Polyamide 12 by Selective Laser Sintering for Water Applications.

The fabrication of metal-organic framework (MOF)-based macro-materials is considered as a promising strategy toward the practical applications of powdered MOF crystals. In this study, selective laser sintering (SLS), an advanced three-dimensional (3D) powder printing technique, has been employed to fabricate MOF-polymer mixed matrix films (MMFs) by using thermoplastic polyamide 12 (PA12) powder as the matrix material and five types of MOFs including ZIF-67, NH2-MIL-101(Al), MOF-801, HKUST-1, and ZIF-8 crystals as the fillers. A three-layer HKUST-1-PA12 complex with a grid pattern is fabricated to demonstrate the printability of 3D MOF-polymer structure. Single-layer MMFs with grid patterns are printed by using the five types of MOF fillers with different mass loadings to study their free-standing characteristic, thickness, specific surface area, hydrophilia, water permeate flux, and mechanical stability. The methylene blue (MB) adsorption tests are conducted using the NH2-MIL-101(Al)-PA12 MMFs with different grid patterns to exemplify the applications of the MMFs for water purification. It is confirmed that the MOF components retain their high maximum adsorption capacity, and the printed MMFs can be conveniently regenerated for cyclic utilization. This work provides an insight into the utilization of advanced 3D printing technology to manufacture macro-MOF-polymer materials for practical applications.

A novel template-free wet chemical synthesis method for economical production of zinc oxide microrods under atmospheric pressure

This work reports a template-free wet chemical synthesis method for economical production of zinc oxide (ZnO) microrods by using an inexpensive polypropylene beaker as a reactor under atmospheric pressure. The morphology and crystal structure of the as-prepared ZnO microrods were investigated by scanning electron microscopy (SEM), X-ray powder diffraction (XRD) and Raman scattering. Fourier-transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), Ultraviolet–visible (UV/Vis) spectroscopy and methylene blue photocatalytic degradation test were also conducted to study their physical and chemical properties. The results show that the as-prepared ZnO microrods have a hexagonal crystal structure. Under the optimal synthesis condition, they have a diameter of 0.8–2.6 μm and a length of 10–40 μm. Compared with commercial ZnO powders, the as-prepared ZnO microrods have a higher whiteness. Meanwhile, the as-prepared ZnO microrods have very low photocatalytic activity, indicating that they have good photostability and are unlikely to cause the photodegradation of polymers such as binders. As a result, they will be an excellent and economical white pigment with great potential in coating applications.

A Review on Microbial Surfactants: Production, Classifications, Properties and Characterization

Surfactants are a surface-active group of molecular compounds with hydrophobic and hydrophilic moieties in one single molecule that distributes themselves between two immiscible fluids, reduce surface/ interfacial tensions and cause the solubility of non-polar compounds in polar solvents. Besides surface and interfacial activities, they display properties such as solubilization, detergency, lubrication, emulsification, stabilization and foaming capacity. Microbiologically derived surfactants are called biosurfactants. They are produced as either metabolic products or as the surface chemistry of an actual cell. The employment of screening techniques such as surface tension measurements, drop collapse test, oil spreading assay, emulsification index (%EI24), cetyltrimethylammonium bromide (CTAB)/methylene blue agar plate test and strain characterization. Others are analytical techniques including liquid chromatography-mass spectroscopy, thin layer chromatography, high-performance liquid chromatography, Fourier transform infrared spectroscopy, nuclear magnetic resonance, fast atom bombardment-mass spectrometry and electrospray ionization-mass spectrometry. These have led to the identification of biosurfactant producing microorganisms, properties and characterization of biosurfactants. Therefore, this review tends to provide the current knowledge of the screening techniques and chromatography/spectroscopic tools employed to study biosurfactants. Results from a detailed study of these tools can unveil new surfactant producing microorganism, decipher chemical diversity and multifunctional properties of biosurfactants critical for applications in diverse industrial sectors.

Prevalence of Anastomotic Leaks and Diagnostic Methods in Sleeve Gastrectomy

Background: Staple line leak is a major complication of sleeve gastrectomy. Intraoperative methylene blue and computer tomography (CT) scan tests are routinely used to evaluate the leakage; however...

Effect of clay in alternative fine aggregates on performance of concrete

The worldwide utilization of alternative fine aggregates (AFA) in the construction sector is increasing exponentially these days due to extensive exploitation of natural sand. However, presence of excessive clay in AFA is considered harmful for fabrication of concrete and mortar. The existence of fine sediments like clay at the interstices of AFA highly influence the performances of concrete. Three types of AFA such as crushed rock sand (CRS), coal bottom ash (CBA) and recycle fine aggregates (RFA) are used in present study. Keeping in view, assessment of clay content in AFA is carried out by methylene blue test and their effects on performance of concrete. In this study, the volume fraction of AFA is varied by 30%, 50%, 75% and 100% respectively. Efforts were also made to develop standard correlation curve of methylene blue value of fine aggregates to corresponding clay content. The methylene blue value for all fine aggregates were well within 6 mg/g and correspondingly less than 5% clay content. Results indicated that MBV greater than or equal to 1.50 is not suitable for preparing concrete. It is also observed that properties and durability of these AFA concrete depend on the amounts of clay.