Concentrations Of Methyl Paraben Research Articles

Urinary concentrations of bisphenols and parabens and their association with attention, hyperactivity and impulsivity at adolescence

BackgroundNeurobehavioural disorder diagnoses have been increasing over the last decades, leading to heightened interest in the aetiological factors involved. Endocrine disrupting chemicals, such as parabens and bisphenols, have been suggested as one of those factors. It is unknown whether exposure during adolescence may affect neurobehavioural development. ObjectiveTo determine whether urinary concentrations of parabens and bisphenols are associated with attention and concentration in adolescents, in general and sex-specific. MethodsWe invited 188 adolescents (13–15 years old) for the follow-up birth cohort-study. Concentrations of five parabens and three bisphenols (BPA; BPF; BPS) were measured in morning urine after overnight fasting, using a validated LC-MS/MS method. Attention and concentration were assessed at the clinic with subtests of the Test of Everyday Attention in Children and the Dutch Attention Deficit Hyperactivity Disorder questionnaire (AVL), the latter being filled in by parents. Linear regression analyses were performed, adjusting for urine creatinine concentrations and potential confounding factors. Results101 (54%) adolescents participated (46 girls; 55 boys). Urinary paraben concentrations were higher in girls than in boys. Methylparaben was positively associated with attention in girls (p ≤ .05; B= −2.836; 95%CI= −5.175;−.497), ethylparaben negatively with hyperactivity (p ≤ .05; B= −1.864; 95%CI= −3.587;−.141). Butylparaben was associated with more optimal scores on parent reported attention. Propylparaben was negatively associated with scores on sustained auditory attention in girls (p ≤ .10; B=.444; 95%CI= −.009;.896). Bisphenol concentrations were not associated with scores on attention and concentration after adjusting for confounders. ConclusionIn 13–15-year-old Dutch adolescents, urinary concentrations of methylparaben and ethylparaben were associated with better attention and less hyperactivity, whereas a trend toward significance was found between higher urinary propylparaben concentrations and poorer attention. Bisphenol concentrations were not associated with attention and concentration after adjusting for confounders.

Selected Essential Oils as Natural Ingredients in Cosmetic Emulsions: Development, Stability Testing and Antimicrobial Activity

Abstract: Background: Essential oils are currently the subject of intensive scientific study, and their potential as active medicinal compounds or natural preservatives has attracted the attention of the cosmetic and pharmaceutical industries. Objectives: The objectives of the present study were: to develop a cosmetic emulsion based on different concentrations of synthetic preservative and essential oils, applied as natural preservatives; to evaluate and subsequently compare their stability and antimicrobial activity. Materials and Methods: Various samples were prepared using different concentrations of methylparaben, and essential oils as well. The fresh samples were evaluated immediately after preparation related to organoleptic and physicochemical testing. The behavior of the samples related to light tests, accelerated tests, and microbiological stability tests as well were performed for four weeks of storage. Results: During the accelerated stability test, formulations loaded with 2% essential oils were not stable as was noted a variation in the color and liquefaction in the second week of storage at 40°C; their rheological behavior showed a significant decrease in the viscosity, varying from 4890±34.7 to 3720±37.9. For all the samples, the pH levels were within the physiological skin pH range, it varied from 6.01±0.33 to 6.92±0.19. The self-preserving activity of all the cosmetic emulsions was satisfactory. Conclusion: Cosmetic emulsions loaded with essential oils, in the concentration of 1% showed promising stability, physico-chemical characteristics, and selfpreserving properties, in different storage conditions. The formulations loaded with essential oils provide a novel alternative for the preservation of “paraben-free” cosmetic products. Keywords: Cosmetic emulsion, Essential oils, Methylparaben, Formulation, Stability testing, Self-preserving activity.

Synthesis of biochar-CoFe2O4 nanocomposite for adsorption of methylparaben from wastewater under full factorial experimental design.

The presence of endocrine-disrupting chemicals in municipal wastewater has emerged as a threat to human health and the environment. Therefore, this study aimed to develop biochar-cobalt ferrite (BCF) nanocomposite for the removal of methylparaben from water under the full factorial experimental design of 4 factors with 3 levels (34). The biochar-CoFe2O4 nanocomposite was developed by co-precipitation method from cobalt ferrite and biochar of Eucalyptus tree bark. Adsorbent surface morphology and functional and elemental composition were carried out by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and energy-dispersive X-ray spectroscopy (EDS) techniques which showed the presence of cracks with a rough surface, reasonable surface chemical composition, and many chemical functional groups, respectively. The experimental and predicted adsorption efficiencies ranged from 25.3 to 85.6% and 21.8 to 80.3%, respectively. The maximum adsorption performance (85.6%) reduced the methylparaben concentration from 27.5 to 4.0mg/L at the optimum condition of adsorbent dose of 55mg/100mL, pH 6, contact time 90min, and the initial methylparaben concentration of 27.5mg/L. However, the adsorbent dose was the most influential main factor whereas the least influential was the interaction between solution pH and contact time under the regression model. The model also showed that 69% methylparaben removal was described by the regression model. The experimental data best fitted with the Freundlich model indicate multilayer adsorption which is the implication of physisorption. The sorption mechanism is attributed to Vander Waals forces, H-bonding, and dipole interaction. This BCF nanocomposite adsorbent appears to be promising for the removal of methylparaben from wastewater, but a further optimization process is essential to boost the treatment performance.

Finger pump microfluidic detection system for methylparaben detection in foods

A novel assay platform consisting of a finger pump microchip (FPM) and a WiFi-based analytical detection platform is presented for measuring the concentration of methylparaben (MP) in commercial foods. In the presented approach, a low quantity (5 μL) of distilled food sample is dripped onto the FPM and undergoes a modified Fenton reaction at a temperature of 40 °C to form a green-colored complex. The MP concentration is then determined by measuring the color intensity (RGB) of the reaction complex using APP software (self-written) installed on a smartphone. The color intensity Red(R) + Green(G) value of the reaction complex is found to be linearly related (R2 = 0.9944) to the MP concentration for standard samples with different MP concentrations ranging from 100 to 3000 ppm. The proposed method is used to detect the MP concentrations of 12 real-world commercial foods. The MP concentrations measurements are found to deviate by no more than 5.88% from the results obtained using a conventional benchtop method. The presented platform thus offers a feasible and low-cost alternative to existing macroscale techniques for measuring the MP concentration in commercial foods.

Urinary phenol concentrations and fecundability and early pregnancy loss.

Are urinary phenol concentrations of methylparaben, propylparaben, butylparaben, triclosan, benzophenone-3, 2,4-dichlorophenol or 2,5-dichlorophenol associated with fecundability and early pregnancy loss? 2,5-dichlorophenol concentrations were associated with an increased odds of early pregnancy loss, and higher concentrations of butylparaben and triclosan were associated with an increase in fecundability. Phenols are chemicals with endocrine-disrupting potential found in everyday products. Despite plausible mechanisms of phenol reproductive toxicity, there are inconsistent results across few epidemiologic studies examining phenol exposure and reproductive function in non-fertility treatment populations. Specimens and data were from the North Carolina Early Pregnancy Study prospective cohort of 221 women attempting to conceive naturally from 1982 to 1986. This analysis includes data from 221 participants across 706 menstrual cycles, with 135 live births, 15 clinical miscarriages and 48 early pregnancy losses (before 42 days after the last menstrual period). Participants collected daily first-morning urine specimens. For each menstrual cycle, aliquots from three daily specimens across the cycle were pooled within individuals and analyzed for phenol concentrations. To assess sample repeatability, we calculated intraclass correlation coefficients (ICCs) for each phenol. We evaluated associations between phenol concentrations from pooled samples and time to pregnancy using discrete-time logistic regression and generalized estimating equations (GEE), and early pregnancy loss using multivariable logistic regression and GEE. ICCs for within-person variability across menstrual cycles in pooled phenol concentrations ranged from 0.42 to 0.75. There was an increased odds of early pregnancy loss with 2,5-dichlorophenol concentrations although the CIs were wide (5th vs 1st quintile odds ratio (OR): 4.79; 95% CI: 1.06, 21.59). There was an increased per-cycle odds of conception at higher concentrations of butylparaben (OR: 1.62; 95% CI: 1.08, 2.44) and triclosan (OR: 1.49; 95% CI: 0.99, 2.26) compared to non-detectable concentrations. No associations were observed between these endpoints and concentrations of other phenols examined. Limitations include the absence of phenol measurements for male partners and a limited sample size, especially for the outcome of early pregnancy loss, which reduced our power to detect associations. This study is the first to use repeated pooled measures to summarize phenol exposure and the first to investigate associations with fecundability and early pregnancy loss. Within-person phenol concentration variability underscores the importance of collecting repeated samples for future studies. Exposure misclassification could contribute to differences between the findings of this study and those of other studies, all of which used one urine sample to assess phenol exposure. This study also contributes to the limited literature probing potential associations between environmental exposures and early pregnancy loss, which is a challenging outcome to study as it typically occurs before a pregnancy is clinically recognized. This research was supported by the National Institute of Environmental Health Sciences of the National Institutes of Health (award number F31ES030594), the Intramural Research Program of the National Institutes of Health, the National Institute of Environmental Health Sciences (project numbers ES103333 and ES103086) and a doctoral fellowship at the Yale School of Public Health. The authors declare they have no competing interests to disclose. N/A.

Occurrence and profile characteristics of environmental phenols in human urine from a rural area in Northwestern China

Many environmental phenols, such as bisphenols, benzophenones and parabens, are known as endocrine disruptors and can adversely affect human health. However, the knowledge of human exposure to common environmental phenols in Chinese rural areas is insufficient. In this context, 181 urine samples were collected from participants in a rural area in Northwest China and were analyzed for nine bisphenols, three benzophenones and four parabens. Bisphenol A (BPA), bisphenol S, benzophenone-1 (BP-1), benzophenone-3 (BP-3), 4-hydroxybenzophenone, methylparaben (MeP), ethylparaben and propylparaben (PrP) were detected in more than 50% of the urine samples, with median concentrations of 0.938 ng/mL, 0.0111 ng/mL, 0.191 ng/mL, 1.30 ng/mL, 0.0320 ng/mL, 25.9 ng/mL, 4.31 ng/mL and 1.94 ng/mL, respectively. A significant positive correlation was observed between BP-1 and BP-3, as well as between MeP and PrP, indicating metabolic transformation and combined use, respectively. The concentrations of MeP and PrP in females were significantly higher than those in males, suggesting that females were exposed to more MeP and PrP than males. Urinary concentrations of BPA, BP-3, MeP and PrP could be influenced by age. Other demographic information, such as annual household income, education and occupation was not associated with the exposure level of the targeted phenols in adults. The estimated daily intakes of the analytes except BPA were all below their respective tolerable/acceptable daily intake levels. This study profiles the demographic differences in the exposure to environmental phenols in general populations from rural areas and provides information on risk assessments.

Human serum paraben levels and their associations with rheumatoid arthritis: a case-control study from Hangzhou, China.

Parabens are widely used in consumer products resulting in frequent exposure to humans. To date, little is known about the association between human paraben exposure and rheumatoid arthritis (RA). In this study, a case-control study (n = 290) was conducted in Hangzhou, China, aiming to quantify the concentrations of methyl paraben (MeP), ethyl paraben (EtP), propyl paraben (PrP), and butyl paraben (BuP) in serum samples and to determine their associations with RA risks. MeP (mean 4.7 ng/mL, range <0.05-20 ng/mL) was the predominant paraben in human serum, followed by PrP (1.9 ng/mL, <0.12-24 ng/mL), EtP (1.4 ng/mL, <0.09-10 ng/mL), and BuP (1.09 ng/mL, <0.10-10 ng/mL). With 1-unit increase of MeP concentrations in human serum, the levels of rheumatoid factors, anticyclic citrullinated peptide antibody, and immunoglobulin G will increase by 0.19 unit (95% confidence intervals [CI]: 0.12-0.46), 0.30 unit (95% CI: 0.26-0.58), and 0.24 unit (95% CI: 0.21-0.30) in the adjusted model, respectively. One-unit increase of MeP and PrP concentrations in human serum was associated with an increase of 0.15 (95% CI: 0.037-0.28) and 0.20 (95% CI: 0.10-0.32) in the C-reactive protein concentrations. In addition, an association between serum MeP levels and the incidence of RA (odds ratios (OR)crude = 1.33, CI: 1.11-1.62, p = 0.03; ORadjusted = 1.86, CI: 1.32-2.63, p = 0.02) was positive and significant. Based on the measurements of serum paraben concentrations, this work supports the evidence for the significant associations among paraben exposure, change of specific immune marker, and RA risks.

Kinetics of zero-valent iron-activated persulfate for methylparaben degradation and the promotion of Cl−

Methylparaben (MP) is an emerging pollutant, and the optimal conditions and kinetics of MP degradation using nano-zero-valent iron-activated persulfate (nZVI/PDS) need to be further investigated. This paper firstly investigated the response surface methodology (RSM) analysis of MP degradation by the heterogeneous system nZVI/PDS and concluded that the initial pH had the most significant effect on MP degradation. The optimal experimental conditions predicted by the RSM were as follows: initial pH 2.75, [nZVI]0 = 2.87 mM, [PDS]0 = 2.18 mM (MP degradation level of 95.30%). First- and second-order kinetic fits were performed for different initial pH levels and different concentrations of MP, nZVI, and PDS. It was determined that k = 0.0365 min−1 (R2 = 0.984) when the initial pH was 3, [PDS]0 = 2 mM, [MP]0 = 20 mg L−1, and [nZVI]0 = 3 mM (MP degradation level of 94.25%). The rest of the conditions were more closely fitted to the second-order reactions. The effects of different concentrations of anions and humic acid (HA) on the MP degradation level and k were examined, and it was found that Cl− could promote MP degradation to 97.69% (increased by 3.65%) and increase the k in accordance with the first-order reaction kinetics (0.0780 min−1, R2 = 0.991). Finally, the analysis of intermediates revealed 5 reaction pathways and 7 reaction intermediates, which inferred a possible reaction mechanism with the recycling performance of nZVI. In this paper, the superiority of nZVI/PDS for the purposes of activating MP degradation was affirmed. The presence of Cl− can enhance the level of MP degradation was confirmed, which provides a new direction for future practical engineering applications.

Maternal diet quality moderates associations between parabens and birth outcomes

Background/objectiveMaternal paraben exposure and diet quality are both independently associated with birth outcomes, but whether these interact is unknown. We assessed sex-specific associations of parabens with birth outcomes and differences by maternal diet quality. MethodsIllinois pregnant women (n = 458) provided five first-morning urines collected at 8–40 weeks gestation, which we pooled for quantification of ethylparaben, methylparaben, and propylparaben concentrations. We collected/measured gestational age at delivery, birth weight, body length, and head circumference within 24 h of birth, and calculated sex-specific birth weight-for-gestational-age z-scores and weight/length ratio. Women completed three-month food frequency questionnaires in early and mid-to-late pregnancy, which we used to calculate the Alternative Healthy Eating Index (AHEI)-2010. Linear regression models evaluated sex-specific associations of parabens with birth outcomes, and differences in associations by average pregnancy AHEI-2010. ResultsIn this predominately non-Hispanic white, college-educated sample, maternal urinary paraben concentrations were only modestly inversely associated with head circumference and gestational length. However, methylparaben and propylparaben were inversely associated with birth weight, birth weight z-scores, body length, and weight/length ratio in female, but not male newborns. For example, each 2-fold increase in methylparaben concentrations was associated with −46.61 g (95% CI: −74.70, −18.51) lower birth weight, −0.09 (95% CI: −0.15, −0.03) lower birth weight z-scores, −0.21 cm (95% CI: −0.34, −0.07) shorter body length, and −0.64 g/cm (95% CI: −1.10, −0.19) smaller weight/length ratio in females. These inverse associations were more prominent in females of mothers with poorer diets (AHEI-2010 < median), but attenuated in those with healthier diets (AHEI-2010 ≥ median). In newborn males of mothers with healthier diets, moderate inverse associations emerged for propylparaben with gestational length and head circumference. ConclusionsMaternal diet may moderate associations of parabens with birth size in a sex-specific manner. Additional studies may consider understanding the inflammatory and metabolic mechanisms underlying these findings.

Hepatotoxicity caused by methylparaben in adult zebrafish

Parabens are a class of aquatic pollutants of emerging concern, among which methylparaben (MeP) causes severe pollution worldwide. However, aquatic toxicology of MeP remains largely unknown, which hinders ecological risk evaluation. In the present study, adult zebrafish were exposed to environmentally realistic concentrations (0, 1, 3, and 10 μg/L) of MeP for 28 days, with objectives to reveal the hepatotoxicity based on transcriptional, biochemical, metabolomics, and histopathological evidences. The results showed that MeP subchronic exposure induced the occurrence of hepatocellular vacuolization in zebrafish. The most severe symptom was noted in 10 μg/L MeP-exposed female liver, which was characterized by rupture of cell membrane and small nuclei. In addition, MeP exposure disturbed the balance between oxidative stress and antioxidant capacity. Lipid metabolism dynamics across gut, blood, and liver system were significantly dysregulated after MeP exposure by altering the transcriptions of lipid nuclear receptors and concentrations of key metabolites. Metabolomic profiling of MeP-exposed liver identified differential metabolites mainly belonging to fatty acyls, steroids, and retinoids. In particular, hepatic concentration of cortisol was increased in male liver by MeP pollutant, implying the activation of stress response. Exposure to MeP also inhibited the synthesis and conjugation of primary bile acid (e.g., 7-ketolithocholic acid and taurochenodeoxycholic acid) in female liver. Furthermore, degradation of biologically active molecules, including retinoic acid and estradiol, was enhanced in the liver by MeP. Overall, the present study highlights the hepatotoxicity caused by MeP pollutant even at environmentally realistic concentrations, which necessitates an urgent and accurate risk assessment.

Exposure to methylparaben at environmentally realistic concentrations significantly impairs neuronal health in adult zebrafish

Methylparaben (MeP) is an emerging aquatic pollutant that is found to impact neural functions. However, it still lacks a comprehensive understanding about its neurotoxicology. The present study exposed adult zebrafish to environmentally realistic concentrations (0, 1, 3, and 10 µg/L) of MeP for 28 days, with objectives to elucidate the neurotoxic effects and mechanisms. Proteomic profiling found that MeP pollutant induced distinct mechanism of neurotoxicity as a function of sex. MeP pollutant appeared to preferentially target the neurotransmission cascade via synapse junctions. In male brain, glutamatergic neural signaling was enhanced by 10 µg/L of MeP in characteristics of higher glutamate neurotransmitter content (by 61.9%) and up-regulated glutamate receptor expression by 2.6-fold relative to the control. In MeP-exposed female brain, biomarker proteins of synapse formation and regeneration had significantly lower abundance, accounting for the blockage of synaptic neurotransmission. Furthermore, under the stress of MeP pollutant, both male and female zebrafish initiated a negative feedback mechanism along stress neuroendocrine axis by down-regulating the transcriptions of corticotropin-releasing hormone and its binding protein, which subsequently decreased blood cortisol concentrations. MeP subchronic exposure also disturbed innate immune function. In particular, significant increases in lipopolysaccharide (LPS) content by 15.6% were caused by MeP exposure in male brain, thereby inducing the synthesis of pro-inflammatory cytokines. In contrast, female brain was able to adaptively up-regulate the protein expression of blood brain barrier to inhibit the infiltration of LPS endotoxin into brain. Overall, the present findings pinpoint the potent neurotoxicity of MeP pollutant even at environmentally realistic concentrations.

Synthesis of cobalt ferrite in one-pot-polyol method, characterization, and application to methylparaben photodegradation in the presence of peroxydisulfate

Cobalt ferrite nanoparticles (CFNPs) have been synthesized using a one-pot-polyol method in the presence of different organic mediums as refluxing solvents. The synthesized CFNPs stability, magnetic property, particle size, and structural variations were characterized using high resolution transmission electron microscopy, X-ray diffraction, Brunauer-Emmett-Teller analysis, superconducting quantum interference device, UV–vis Diffuse Reflectance Spectroscopy, and X-ray photoelectron spectroscopy. The CFNPs synthesized in the presence of triethylene glycol achieved the highest saturation magnetization (Ms) 126.7 emu/g. It has been observed that synthesis parameters have significant impacts on the Ms, physical and chemical properties of CFNPs, and need to be explored further in future work. The photocatalytic properties of the synthesized CFNPs were investigated at pH 7, under visible light, on the degradation of methylparaben (MeP). The photocatalytic result shows almost a complete degradation of 10 mg/L MeP within 100 min (99.9%), in the presence of 0.2 g/L CFNPs and 2 mM ammonium peroxydisulfate (APDS), while irradiated by visible light. The effect of operating parameters such as pH, APDS concentration, MeP concentration, catalyst amount, and reactive oxygen species were investigated. The photodegradation kinetics are well fitted to the pseudo-first-order kinetics model. The Total Organic Carbon results showed that 81.7% of MeP was completely mineralized, and the photocatalyst showed good stability even after five cycles. The degradation by-products were identified using Ultra-High-Performance Liquid Chromatography/quadrupole time-of-flight mass spectrometry and a degradation pathway was proposed.

Significant impairment of intestinal health in zebrafish after subchronic exposure to methylparaben

Methylparaben (MeP) is a ubiquitous pollutant in aquatic environment, which has caused severe pollution worldwide. However, aquatic toxicology of MeP is still largely unknown. In the present study, adult zebrafish were exposed to environmentally realistic concentrations of MeP (0, 1, 3, and 10 μg/L) for 28 days. In terms of the antimicrobial nature, dysregulation of gut microbiota and zebrafish health by MeP were elucidated after exposure. High-throughput amplicon sequencing showed that MeP subchronic exposure was able to disrupt the composition and diversity of gut microbial community, which was characterized by the alterations in alpha diversity and divergent distribution by principal component analysis. In addition, MeP exposure increased the body length and body weight of female fish, implying stimulated growth at low doses. In male intestine, consistent increases were notable in goblet cell density, tight junction protein (TJP) 2 expression, and serotonin neurotransmitter concentration after MeP exposure. In contrast, female intestine exposed to MeP had lower density of goblet cells, inhibited expression of TJP2, reduced concentration of serotonin, but up-regulated transcription of pro-inflammatory cytokine. Under the stress of MeP pollutant, intestinal catalase antioxidant enzyme was activated, thus contributing to the removal of oxidative free radicals. Correlation analysis verified the modulation of TJP2 expression by Lactobacillus probiotic bacteria. Disturbances in goblet cell, tight junctions, and serotonin by MeP may be combined to interfere with gut barrier function. Overall, the present study highlights the impairment of intestinal health by environmentally realistic concentrations of MeP, which necessitates an urgent risk assessment.

The potential implications of estrogenic and antioxidant-dependent activities of high doses of methyl paraben on MCF7 breast cancer cells.

Methyl paraben (MP) is an endocrine-disrupting compound that possesses estrogenic properties and contributes to an aberrant burden of estrogen signaling in the human breast and subsequently increasing the risks for the development of breast cancer. The exact exposure, as well as the safe concentrations, are variable among daily products. The present study addresses the effects of exposure to escalated concentrations of MP on the proliferation of MCF-7 breast cancer cells in addition to exploring its other mechanisms of action. The study involved exposure of cultured MCF-7 breast cancer cells to seven MP concentrations, ranging from 40 to 800 µM for 5 days. Cell viability, apoptosis, and proliferation were respectively assessed using crystal violet test, flow cytometric analysis, and quantitative real-time polymerase chain reaction for Ki-67 expression. The estradiol (E2) secretion and oxidative stress were also assessed and analyzed in correlation to MP's proliferation and cytotoxicity potentials. The results showed that the maximum proliferative concentration of MP was 800 µM. At a concentration of 40 μM and higher, MP induced increased expression of Ki-67, denoting enhanced proliferation of the cells in monolayer culture. A positive correlation between the detrimental oxidative stress effect of MP's tested concentrations, cell proliferation, and viability was demonstrated (p < 0.05). Our results indicated that MP at high doses induced sustained cell proliferation due to E2 secretion as well as its antioxidant activity. Accordingly, it was concluded that high and unpredicted exposure to MP might carry a carcinogenic hazard on estrogen receptor-positive breast cancer cells.

The Effect of Methylparaben on Development and Fecundity of Drosophila melanogaster

Parabens are a group of chemicals used as preservatives in many industrial products such as foods, cosmetics, and pharmaceuticals. Recent studies have revealed that these chemicals have endocrine disrupting properties. Drosophila melanogaster is one of the suitable model organisms used to test the toxic effect of various chemicals in vivo. In this study, the effects of methylparaben (MP) (50 mM, 100 mM, and 200 mM), one of the most commonly used parabens, on the developmental parameters and fecundity of D. melanogaster were examined. No significant difference was observed in the viability rates from larva to pupa and from pupa to adult. However, experimented MP concentrations caused a delay in larva to pupa and pupa to adult developmental times (p<0.05). While MP did not cause a significant change in mean offspring number, it significantly decreased fecundity at all doses (p<0.05).

Occurrence and human exposure assessment of parabens in water sources in Osun State, Nigeria

Parabens are chemicals extensively used in pharmaceuticals, cosmetics, personal hygiene and food products as preservatives. They are classified as emerging contaminants with endocrine-disrupting capability. In this study, the concentrations of Methylparaben (MeP), Ethylparaben (EtP), Propylparaben (PrP) and Butylparaben (BuP) were obtained from groundwater, surface-water and packaged water samples collected from urban and rural areas of Osun State, Nigeria using HPLC-UV equipment. Data obtained were subjected to descriptive (Mean ± SD), inferential (Kruskal-Wallis test) and multivariate analyses. MeP had the highest average concentration of 163 and 68 μg L−1 in surface water and groundwater respectively while concentrations of MeP, EtP, PrP and BuP were higher than previously reported in other countries. Methylparaben had the highest detection frequencies (88.0 and 50.0%) followed by BuP (69.0 and 50.0%) in surface water and groundwater respectively. No significant difference was observed for concentrations of parabens in groundwater samples in urban and rural sampling sites, suggesting that people living around these sites are equally exposed to any health implications from the use of paraben-polluted potable water. Principal Component Analysis (PCA) data suggest that the pairs MeP & EtP, PrP & BuP (in surface water samples) and MeP, EtP, & PrP (in groundwater samples) are from similar pollution sources. Ecological risk assessment using Algae, Fish, and Daphnia suggests Daphnia as the most sensitive organism while BuP and PrP show the highest health risk. Human exposure assessment showed that higher overall median estimated daily intake (EDI) values for groundwater were observed in infants (1.71 μg kg−1 bw day−1, ∑PBs) compared to toddlers (1.03 μg kg−1 bw day−1, ∑PBs), children (0.64 μg kg−1 bw day−1, ∑PBs), teenagers (0.51 μg kg−1 bw day−1, ∑PBs) and adults (0.62 μg kg−1 bw day−1, ∑PBs). Although these values are below limits set in a few countries, potential bioaccumulation could lead to severe health consequences.

Alternanthera spp. based-phytoremediation for the removal of acetaminophen and methylparaben at mesocosm-scale constructed wetlands

Recently, the spread of pharmaceuticals and personal care products (PPCPs) in the aquatic environment has steadily increased. In this study, phytoremediation technology, using an ornamental plant (Alternanthera spp.), was investigated to improve the removal of acetaminophen (AC) and methylparaben (MP) from a synthetically prepared wastewater. Three exposure lines (AC-line, MP-line and control-line) were performed with a total of 26 subsurface-horizontal constructed wetlands (SSH-CWs) that operated in batch feeding mode. The influence of plants in addition to the initial spiking concentration (20, 60 and 100 mg/L) of AC and MP on the removal efficiency was evaluated throughout the 35-days experiments. The highest removal efficiencies for AC and MP were 88.6% and 66.4%, respectively, achieved in the planted CWs; whereas only 29.7% and 21.9% were achieved in the control CWs for AC and MP, respectively. The results confirmed the role of Alternanthera spp. for accelerating the removal of AC and MP from synthetically contaminated wastewater in CWs.

Association of prenatal exposures of parabens with children’s obesity－A longitudinal cohort study

BACKGROUND AND AIM: The association between prenatal exposure of parabens and children’s obesity was inconclusive and we aimed to exam it. METHODS: Mother-newborn pairs were enrolled at the 3rd trimester during pregnancy and followed as children aged 2-3, 5-6, 8-9, 11-12, 14-15, and 17-18 years in central Taiwan. Information were collected via questionnaires, physical examinations, and bio-samples. Urinary concentrations of methyl paraben (MP), ethyl paraben (EP), propyl paraben (PP), butyl paraben (BP) in pregnant women and 17-18 years-old children were determined using liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) and corrected by urinary creatinine level. Molar concentrations of the four parabens were summed to ΣPB. Children’s body mass index (BMI) were calculated at each follow-up. Ordinary linear regression model was performed. RESULTS:Prenatal exposure levels of MP, EP, PP, BP, and ΣPB were similar in 92 male and 99 female children. MP was with the highest and EP was with the lowest concentration. After adjustment for child sex, annual family income, parental education level, maternal BMI, and weight gain, the associations of children’s BMI with prenatal exposure of MP and ΣPB were positive though non-significant at ages of 2-3 years and null at ages of 5-6 years. The associations turned to be negative since ages of 8-9 years and borderline significant at ages of 14-15 years. In analyses at ages of 17-18 years, we additionally adjusted for children’s paraben exposure levels and found that per log10 μg/g MP and ΣPB increase in-utero were significantly associated with 1.20 and 1.32 reductions of BMI (p=0.022 and 0.017). CONCLUSIONS:MP was the most common paraben the pregnant women exposed in central Taiwan. The association between prenatal exposure of parabens and children’s BMI may vary with age, it tended to be positive in toddlers and negative from puberty to post-puberty. KEYWORDS: Obesity and metabolic disorders, Children's environmental health, Endocrine disrupting chemicals, Environmental epidemiology, Food/nutrition, Birth outcomes

Urinary level of un-metabolized parabens in women working in beauty salons

Parabens are a group of chemical additive extensively utilized in various health care products and ubiquitously observed in the different environmental matrixes. Nevertheless, the exposure of women working in beauty salons to these pollutants is not well-documented. For this purpose, 50.00 women working in beauty salons were chosen as the exposed group (EG) and 35.00 housewives were chosen as the control group (GC). The concentration of methyl paraben (MeP), ethyl paraben (EtP), butyl paraben (BuP), propyl paraben (PrP), benzyl paraben (BzP), heptyl paraben (HepP), and para-hydroxybenzoic acid (4-HB) metabolite were quantified in the collected urine samples. It was seen that paraben sexist with a high detection frequency (DF) in the urine of women working in beauty salons. The results also revealed that the significant difference between the urinary parabens level in the EG and CG (P value < 0.05). The median concentration of Σparaben and HB-4 metabolite in the before exposure (BE) samples was 124.00 and 219.00 μg/L, while in the after exposure (AE) samples, it was 156.00 and 249.00 μg/L, respectively. Moreover, the parabens levels in the AE samples were considerably higher than in BE samples in women working in beauty salons (P value < 0.05). This research also documented that “personal care products (PCPs) usage” can be known as a leading factor for the urinary paraben level in the studied individuals. The median total estimated daily intakes (TEsDI) for MeP, EtP, and PrP for the studied women were obtained as 8.02, 4.57, and 7.88 μg/L respectively. Also, a significant and positive association was observed between EtP, PrP as well as BuP and 8-OhdG (as a DNA oxidative stress biomarker) (P value < 0.01). Further, a significant and positive association was found between EtP as well as BuP and some biomarkers of kidney damage (like uTIMP-1 and uKim-1). Accordingly, it can be stated that women working in beauty salons are at a high risk in terms of DNA oxidative stress and kidney damage.

Determination of six parabens in biological samples by magnetic solid-phase extraction with magnetic mesoporous carbon adsorbent and UHPLC-MS/MS

Although parabens are useful due to their antiseptic properties, their widespread use has caused concerns regarding their potential toxicological effects. In this study, a novel magnetic solid-phase extraction combined with ultra-high-performance liquid chromatography-tandem mass spectrometry (MSPE-UHPLC-MS/MS) was developed, based on ordered magnetic mesoporous carbon (MMC), for paraben analysis. The MMC was prepared by soft-template synthesis, with a unique pore structure and a highly specific surface response, indicating potential as an excellent adsorbent. Several parameters affecting the paraben extraction efficiency were investigated and a novel method for paraben analysis in serum and urine samples using MSPE-UHPLCMS/MS was developed. The concentrations of methylparaben, ethylparaben, isopropylparaben, and propylparaben in these samples were 0.0380–4.36, 0.460–9.65, 0.0118–0.770, and 0.0363–0.641 μg/L, respectively, whereas isobutylparaben and butylparaben were not detected. Furthermore, satisfactory recoveries of 76.4–121% with relative standard deviations (n = 5) of 1.9–8.6% were obtained. Therefore, the developed MSPE-UHPLC-MS/MS method was efficient, highly sensitive, and reliable for analysing parabens in complex biological samples.