Method For Rejuvenation Research Articles

FAT GRAFTING FOR AESTHETIC AND RECONSTRUCTIVE FACIAL PLASTIC SURGERY: A LITERATURE REVIEW

Introduction: One of the biggest reasons for an aging face is the loss of facial volume in both the bony and soft tissues. Fat grafting has become popular, and many investigators confirmed the utility of fat grafting for facial rejuvenation. Fat grafting is thought to be an ideal method for facial rejuvenation because it is effective not only for volume augmentation, but also for improving skin quality. Objective: The objective of this article is to present a literature review of 27 studies published in the last 10 years (2013-2023), performed following previously determined inclusion and...

Rejuvenation of Coastal Sandy Soils through Organics

The coastal zone represents the transition from terrestrial to marine influences and vice versa. They are predominated by sandy soil texture, and have low agricultural productivity due to their properties and are vulnerable to erosion. Effective maintenance of soil organic matter is found to be a pre-requisite to ensure soil health and crop productivity. Therefore, organics based rejuvenation methods for coastal sandy soils need to be developed. A pot culture experiment was done at Kochuveli, Industrial area, in the coastal belt of Thiruvananthapuram district, Kerala, with Amaranthus (variety: Arun) as test crop. The experiment was conducted in a completely randomized design (CRD) with 12 treatments and 3 replications. In the study, effect of different organic manures such as FYM, coirpith compost, vermicompost, city compost, biochar and thermochemical organic fertilizer (TOF), with and without lime on plant growth and yield, soil organic carbon and nutrient dynamics in the southern coastal sandy soils were studied. Soil management using organic manures had considerable impact on the growth and yield characteristics of the crop. Also the analyses of post-harvest soil for nutrient parameters showed that the highest value for organic carbon, nitrogen and potassium were recorded in soil treated with lime+TOF, while the highest value for available phosphorous was recorded in treatment lime+vermicompost. The study showed that soil test based lime application and basal application of TOF @ 25 t ha-1, top dressing with fresh cow dung slurry @ 1kg per 10 litres, along with 272.7 kg rock phosphate and 122.5 kg potassium sulphate ha-1 was the best treatment to improve the soil quality, growth and yield of amaranthus in coastal sandy soils in Thiruvananthapuram district.

Microneedling in Dermatology: A Comprehensive Review of Applications, Techniques, and Outcomes.

Microneedling, also known as collagen induction therapy, is a minimally invasive dermatological procedure that has gained widespread popularity for treating various skin conditions, including acne scars, wrinkles, hyperpigmentation, and stretch marks. By creating controlled micro-injuries in the skin, microneedling stimulates the body's natural healing processes, resulting in increased collagen and elastin production, essential for maintaining skin elasticity and firmness. Over the past few decades, microneedling has evolved significantly, with advancements such as automated devices, radiofrequency microneedling, and combination therapies enhancing its effectiveness and safety profile. This comprehensive review explores the mechanisms of action, various techniques, and clinical applications of microneedling, highlighting its advantages over other skin rejuvenation methods. The review also examines patient satisfaction, safety considerations, and potential complications, providing a balanced perspective on its clinical utility. Furthermore, the discussion includes future directions in microneedling technology and research, focusing on emerging innovations and potential new applications. As the field advances, microneedling is poised to play an increasingly important role in aesthetic medicine, offering a reliable and effective solution for skin rejuvenation and beyond. This review is a valuable resource for healthcare professionals, guiding the optimization of microneedling practices and informing future research efforts.

“Traitors to Their Profession”: Popular Media and the Repudiation of Sakaki Yasusaburō’s Rejuvenation Study in the 1920s

In 1921, Sakaki Yasusaburō, a professor of psychiatry at Kyushu Imperial University, introduced to Japanese audiences a rejuvenation method invented by the Austrian physiologist Eugen Steinach. Four years later, Sakaki was forced to resign from his position due to an embezzlement scandal, leading to the academic repudiation of his rejuvenation studies. This paper investigates the reasons behind this decline and identifies the role of popular media in shaping the professional identities of biomedical researchers in post-WWI Japan. It argues that a series of confrontations concerning the rules and norms of academic behavior that eventually led to Sakaki being labeled a “traitor” to his profession was as much influenced by the popular media as by considerations of scientific rigor. Highlighting the previously understudied interaction between popular media and its impact on the professionalization of biomedical research in modern Japan, this paper reveals the active and multiple roles that popular media plays in determining legitimate research topics and appropriate conduct for biomedical researchers.

Artificial mitochondrial transplantation (AMT) reverses aging of mesenchymal stromal cells and improves their immunomodulatory properties in LPS-induced synoviocytes inflammation

Nowadays, regenerative medicine techniques are usually based on the application of mesenchymal stromal cells (MSCs) for the repair or restoration of injured damaged tissues. However, the effectiveness of autologous therapy is limited as therapeutic potential of MSCs declines due to patient's age, health condition and prolonged in vitro cultivation as a result of decreased growth rate. For that reason, there is an urgent need to develop strategies enabling the in vitro rejuvenation of MSCs prior transplantation in order to enhance their in vivo therapeutic efficiency.In presented study, we attempted to mimic the naturally occurring mitochondrial transfer (MT) between neighbouring cells and verify whether artificial MT (AMT) could reverse MSCs aging and improve their biological properties. For that reason, mitochondria were isolated from healthy donor equine adipose-derived stromal cells (ASCs) and transferred into metabolically impaired recipient ASCs derived from equine metabolic syndrome (EMS) affected horses, which were subsequently subjected to various analytical methods in order to verify the cellular and molecular outcomes of the applied AMT.Mitochondria recipient cells were characterized by decreased apoptosis, senescence and endoplasmic reticulum stress while insulin sensitivity was enhanced. Furthermore, we observed increased mitochondrial fragmentation and associated PARKIN protein accumulation, which indicates on the elimination of dysfunctional organelles via mitophagy. AMT further promoted physioxia and regulated autophagy fluxes. Additionally, rejuvenated ASCs displayed an improved anti-inflammatory activity toward LPS-stimulated synoviocytes. The presented findings highlight AMT as a promising alternative and effective method for MSCs rejuvenation, for potential application in autologous therapies in which MSCs properties are being strongly deteriorated due to patients' condition.

Different impact of recovery annealing and thermal pressure treatments on the microstructure and mechanical properties of rejuvenated Cu64Zr36 metallic glasses: A molecular dynamics study

The interpenetrating connection of icosahedra network (ICOIN) was termed as structural skeleton, which could quantify the strength and ductility of MGs. The impact of different rejuvenation methods on medium-range order (MRO) alterations was rarely studied. This work employed molecular dynamics simulations to examine the difference in MRO evolution of metallic glasses treated by recovery annealing and thermal-pressure treatment during uniaxial tension. All of the specimens obtain enhanced ductility due to improved potential energy. During tension, ICOI of all rejuvenated specimens exhibits an increased destruction rate and a decreased transformation rate. However, there are some converse characteristics in the specimens under different rejuvenation methods. The rejuvenated specimens treated by recovery annealing exhibit increased free volume and decreased ICOIN density. After thermal-pressure treatment, the rejuvenated specimens demonstrated decreased free volume and enhanced ICOIN density. During tension, the enhancement of icosahedral free volume in specimens treated by thermal pressure largely decreased the ICOIN density, thereby increasing the ductility. After tension, the rejuvenated specimens under high pressure have a significant reduction in ICOIN density compared to those under recovery annealing. The significance of this work is to accurately regulate the ICOIN density of rejuvenated specimens.

Ultrasound rejuvenation for upper facial skin: A randomized blinded prospective study.

Growing demand for facial rejuvenation drives advancements in these therapies, including laser, radiofrequency, and focused ultrasound, alongside thermal stimulation adjuncts. These methods, known for stimulating collagen regeneration, skin tightening, and lifting, have gained popularity due to their minimal side effects, low trauma, and high safety, demonstrating favorable outcomes in clinical practice. We sought to assess the efficacy of ultrasound skin tightening for brow lift within the scope of a procedure addressing facial sagging across the entire face. Our aim was to explore a noninvasive method capable of effectively enhancing mild to moderate brow ptosis by tightening and lifting the skin in the upper facial region. This was a rater-blinded, prospective cohort study. The upper facial region of the participants was treated with the new device, micro-focused ultrasound (MFU), in model D3.0/D2.0/M3.0. Outcomes of brow lift were measured in comparison of pretreatment and posttreatment photographs and three-dimensional (3D) vector analysis. A total of 42 participants (37 females) were enrolled, with 2 participants withdrawing from the trial, resulting in 40 subjects who completed 180-day-follow-up and evaluation. 35 (87.5%) were deemed to have clinically significant brow elevation by two blinded assessors (experienced clinicians) at 180-day posttreatment (p < 0.01). The mean change in brow height after 90-day was 2.16 ± 0.63 mm at the frontal position (straight-ahead gaze) (p < 0.01). The 3D vector analysis reveals varying magnitudes of vector displacement in the upward and outward directions of the skin on the frontal region above the eyebrows. Focused ultrasound appears to be a safe and effective method for upper facial skin rejuvenation. A single focused ultrasound treatment on the forehead and temple areas resulted in an average brow elevation of 2.1 mm.

Comparison of the fractionated Nd: YAG 1064-nm picosecond laser with holographic optics and the fractional CO2 laser in atrophic acne scar treatment: a prospective, randomized, split-face study.

The 1064-nm picosecond laser with holographic optics demonstrated significant efficacy in treating atrophic acne scars. Picosecond lasers with fractionated optics have enabled the development ofa breakthrough skin rejuvenation method. The authors compared the fractionated, non-ablative neodymium-doped yttrium aluminum garnet 1064-nm picosecond laser with holographic optics and the fractional CO2 laser in treating atrophic acne scars. One side of each patient's face was randomly allocated and treated with three sessions of the 1064-nm picosecond laser with holographic optics at 2-month intervals. In contrast, the other side was treated with the fractional CO2 laser. Participants were followed up 3 months after the final session. The primary outcome included the physicians' evaluation using the ECCA grading scale and a four-point scale to assess improvement. The patients' assessment of progress, their overall satisfaction and preferences, and the side effects were also evaluated. No significant difference was observed between the two lasers in terms of the mean ECCA scores after treatments (P = 0.209). The physicians' improvement assessment was more significant for the fractional CO2 laser (P = 0.001). The patients' evaluation of improvement and subjective satisfaction were consistent with physicians' four-point scale results. The picosecond laser side had fewer adverse effects (P < 0.001). The fractionated, non-ablative Nd: YAG 1064-nm picosecond laser with holographic optics and the fractional CO2 laser were effective and safe in treating atrophic acne scars. Significantly better clinical outcomes were observed with the fractional CO2 laser, whereas fewer adverse effects were noted with the 1064-nm picosecond laser with holographic optics.

The Endo-lift Laser (Intralesional 1470 nm Diode Laser) for Dermatological Aesthetic Conditions: A Systematic Review.

The prominence of minimally invasive aesthetic approaches has become increasingly pivotal. The endo-lift laser method is an intralesional 1470nm diode laser connected to a fiber that serves both therapeutic and aesthetic properties. We sought to evaluate the efficacy and safety of the endo-lift laser method for dermatological aesthetic applications. PubMed, Ovid-Embase, and Web of Science were systematically searched up to November 5th, 2023. A citation search was also performed. The National Institute of Health (NIH) Quality Assessment Tool was used to evaluate the quality of the studies. Out of 339 articles, twenty-three relevant studies were included in the current review. Applying the endo-lift laser method for rejuvenation, including face and neck lifting, enhancing skin laxity, and disappearing wrinkles, folds, and lines, demonstrated favorable efficacy and safety profile. Moreover, most studies have shown that the endo-lift laser method is promising in eliminating the adipose tissue in the jowl, abdomen, thighs, and arms. The endo-lift laser technique was also efficacious in nose remodeling and blepharoplastic procedures, including treating eyelid and eyebrow ptosis, eye bag, eyebrow position, and eyelid laxity. Patients who suffer from several diseases, such as hidradenitis suppurativa, progressive lipodystrophy, acne vulgaris, scars, and keloids, benefit from procedural treatment with the endo-lift laser technique. Across all studies, the adverse events were mild and self-limiting. Investigating the endo-lift laser method in all aesthetic and therapeutic indications resulted in high patient satisfaction rates. The endo-lift laser technique has therapeutic effects and is recommended for various dermatological aesthetic indications. Further clinical studies with control groups and larger sample sizes are needed to acquire more reliable evidence. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Electrical rejuvenation of chronically implanted macroelectrodes in nonhuman primates

Objective. Electrodes chronically implanted in the brain undergo complex changes over time that can lower the signal to noise ratio (SNR) of recorded signals and reduce the amount of energy delivered to the tissue during therapeutic stimulation, both of which are relevant for the development of robust, closed-loop control systems. Several factors have been identified that link changes in the electrode-tissue interface (ETI) to increased impedance and degraded performance in micro- and macro-electrodes. Previous studies have demonstrated that brief pulses applied every few days can restore SNR to near baseline levels during microelectrode recordings in rodents, a process referred to as electrical rejuvenation. However, electrical rejuvenation has not been tested in clinically relevant macroelectrode designs in large animal models, which could serve as preliminary data for translation of this technique. Here, several variations of this approach were tested to characterize parameters for optimization. Approach. Alternating-current (AC) and direct-current (DC) electrical rejuvenation methods were explored in three electrode types, chronically implanted in two adult male nonhuman primates (NHP) (Macaca mulatta), which included epidural electrocorticography (ECoG) electrodes and penetrating deep-brain stimulation (DBS) electrodes. Electrochemical impedance spectroscopy (EIS) was performed before and after each rejuvenation paradigm as a gold standard measure of impedance, as well as at subsequent intervals to longitudinally track the evolution of the ETI. Stochastic error modeling was performed to assess the standard deviation of the impedance data, and consistency with the Kramers–Kronig relations was assessed to evaluate the stationarity of EIS measurement. Main results. AC and DC rejuvenation were found to quickly reduce impedance and minimize the tissue component of the ETI on all three electrode types, with DC and low-frequency AC producing the largest impedance drops and reduction of the tissue component in Nyquist plots. The effects of a single rejuvenation session were found to last from several days to over 1 week, and all rejuvenation pulses induced no observable changes to the animals’ behavior. Significance. These results demonstrate the effectiveness of electrical rejuvenation for diminishing the impact of chronic ETI changes in NHP with clinically relevant macroelectrode designs.

Comparison of Fat Transfer Combined with Plasma Energy and Only Fat Transfer Methods for Genital Rejuvenation.

To compare the fat transfer combined with plasma energy and only fat transfer methods for genital rejuvenation and to investigate the efficacy enhancing properties of plasma energy. Forty-six patients were equally divided into two groups according to the surgical method as the group A (n = 23) and the group B (n = 23). The patients in the group A received only fat transfer, while the patients in the group B received fat transfer combined with plasma energy. Both groups were scheduled for postoperative follow-up at 1, 3, 6, and 12 months. The lifting effect on the labia majora after the procedure was evaluated with photographs and patient satisfaction questionnaires including the female genital self-image scale (FGSIS). The mean age of all participants was 32.8 ± 5.1 years, and the mean body mass index (BMI) was 24.7 ± 3.4kg/m2. The mean preoperative FGSIS scores were similar between the groups (p = 0.542). The mean total FGSIS score was 18.8 ± 1.4 in the group A and 18.3 ± 1.5 in the group B in the preoperative period. However, the mean FGSIS scores at 1, 3, and 6 months were significantly higher in the group B than the group A (p = 0.032, p = 0.012, and p = 0.009, respectively). At 6 months of follow-up, the mean total FGSIS score was 20.7 ± 1.4 in the group A and 22.3 ± 1.5 in the group B, indicating a statistically significant difference (p = 0.028). This novel technique is a more minimally invasive technique compared to other energy modalities with lower lateral and vertical energy dissipation than other conventional methods, and labia majora fat filling augmentation application can be performed with more permanent and longer-lasting outcomes than fat transfer only. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

Radiofrequency Treatment Attenuates Age-Related Changes in Dermal-Epidermal Junctions of Animal Skin.

The dermal-epidermal junction (DEJ) is essential for maintaining skin structural integrity and regulating cell survival and proliferation. Thus, DEJ rejuvenation is key for skin revitalization, particularly in age-related DEJ deterioration. Radiofrequency (RF) treatment, known for its ability to enhance collagen fiber production through thermal mechanisms and increase heat shock protein (HSP) expression, has emerged as a promising method for skin rejuvenation. Additionally, RF activates Piezo1, an ion channel implicated in macrophage polarization toward an M2 phenotype and enhanced TGF-β production. This study investigated the impact of RF treatment on HSP47 and HSP90 expression, known stimulators of DEJ protein expression. Furthermore, using in vitro and aged animal skin models, we assessed whether RF-induced Piezo1 activation and the subsequent M2 polarization could counter age-related DEJ changes. The RF treatment of H2O2-induced senescent keratinocytes upregulated the expression of HSP47, HSP90, TGF-β, and DEJ proteins, including collagen XVII. Similarly, the RF treatment of senescent macrophages increased Piezo1 and CD206 (M2 marker) expression. Conditioned media from RF-treated senescent macrophages enhanced the expression of TGF-β and DEJ proteins, such as nidogen and collagen IV, in senescent fibroblasts. In aged animal skin, RF treatment increased the expression of HSP47, HSP90, Piezo1, markers associated with M2 polarization, IL-10, and TGF-β. Additionally, RF treatment enhanced DEJ protein expression. Moreover, RF reduced lamina densa replication, disrupted lesions, promoted hemidesmosome formation, and increased epidermal thickness. Overall, RF treatment effectively enhanced DEJ protein expression and mitigated age-related DEJ structural changes by increasing HSP levels and activating Piezo1.

Increasing Robusta Coffee Production Through a Rejuvenation Agrotechnology System in The Politeknik Negeri Jember Collection Garden

Robusta coffee is more produced because of the wider area of Arabica coffee. However, coffee productivity is not offset by plant maintennace. Pruning coffee plants is one way of rejuvenating coffee plants that coffee farmers can do to maintain their productivity a part from replanting. This study was conducted to determine the effect of agrotechnology rejuvination on robusta coffee clones BP 42, BP 234, BP 409, and BP 534 in the collection garden of Politeknik Negeri Jember which are no longer productive so with a 100% rejuvenation system for each clone is carried out. The research method was carried out by observation of coffee plants and shade plants, Foccus Group Discussion (FGD), training and assistance in the implementation of pruning. Temperature and humidity observations are carried out every 2 days in the morning, afternoon and evening with 3 repetitions. The results showed that the rejuvenation method was said to be successful, because the four clones namely BP 42, BP 234, BP 409, and BP 534 produced good shoot growth. The average good growth of shoots was found in BP 409 and BP 534 clones of 100%, followed by BP 42 and BP 234 clones of 94.4%.

LA GREFFE DES CELLULES ADIPEUSES DANS LESTHETIQUE DE LA FACE: TECHNIQUE ET INDICATIONS

The objective of our study is to highlight the technique, the different indications, and the results of autologous fat grafting in facial aesthetics by reporting the experience of our department and through a literature review. This is a retrospective study involving 14 patients who underwent aesthetic and reparative facial fat grafting over a period of 2 years, from January 1, 2022, to January 1, 2024, at the Department of Plastic, Reconstructive, Aesthetic, and Burn Surgery of the Mohammed VI University Hospital Center in Marrakech. We collected 22 autologous facial fat grafts, with the mean age of our patients being 26.36 years, ranging from 11 to 58 years. A clear female predominance was noted (86.4%) with a female-to-male ratio of 6. The two main indications were atrophy and volume enhancement (72%) and contour refinement (28%). The procedures were performed under general anesthesia. The fat was harvested from a single donor site: the abdominal region. The mean volume harvested was 96.8 ml, ranging from 80 ml to 120 ml. All samples were centrifuged at a speed of 3,000 rpm for 3 minutes. The mean injected volume was 25.55 ml, ranging from 5 to 90 ml. 10% of the fat grafts were associated with a facelift. The average length of hospital stay was 1 day. None of our patients experienced immediate or late complications. 68% of patients underwent a single fat grafting session, 21% underwent 2 sessions, and only 11% required 3 sessions. The mean interval between sessions was 3 months. Subjective evaluation of satisfaction 3 months after the last intervention revealed a satisfaction rate of 90%. Adipose cell grafting is currently considered an ideal method for facial rejuvenation and reshaping due to its safety, simplicity, reproducibility, and the durable and natural results it achieves.

Disastrous cerebral and ocular vascular complications after cosmetic facial filler injections: a retrospective case series study

Soft tissue filler injections are among the most popular facial rejuvenation methods. Cerebral infarction and ophthalmic artery occlusion are rare and catastrophic complications, especially when facial cosmetic fillers are injected by inexperienced doctors. Radiologists and plastic surgeons need to increase their awareness of the complications associated with fillers, which allows early diagnosis and intervention to improve patient prognosis. Regarding the mechanism by which vascular occlusion occurs after facial filler injections, a retrograde embolic mechanism is currently the predominant theory. Numerous case reports have been presented regarding complications associated with injections of facial aesthetics. However, the small sample sizes of these studies did not allow for an adequate assessment of the clinical and imaging manifestations based on the location of the occlusion and the type of filler, and detailed elaboration of multiple cerebral infarctions is also lacking. Therefore, this study aimed to investigate the clinical and radiological features of severe cerebral and ocular complications caused by cosmetic facial filler injections. In addition, we discuss the pathogenesis, treatment, and prognosis of these patients. The clinical, computed tomography (CT), magnetic resonance imaging (MRI), and digital subtraction angiography (DSA) findings were described and analysed. Radiological examinations are crucial for demonstrating severe complications, and brain MRI is especially strongly suggested for patients with cosmetic filler-induced vision loss to identify asymptomatic cerebral infarctions. Extreme caution and care should be taken during facial injections by plastic surgeons.

Fractional CO2 Laser for Vulvar Tissue Rejuvenation: A Prospective Study.

Objective: Aging and changes in hormone levels influence the appearance of the vulva, including the texture, pigmentation, and other manifestations, all of which may largely affect the physical and mental health of women. This study aimed to evaluate the efficacy and safety of fractional carbon dioxide (CO2) laser treatment for vulvar rejuvenation in Chinese women. Background: The limited options currently available for vulvar rejuvenation raise concerns. There is insufficient evidence to determine whether the fractional CO2 laser can safely and effectively rejuvenate the vulvar area for women of various ages and races. Methods: The study included 17 patients (mean age = 36.4 years) treated three times by continuous fractional CO2 laser with an interval of 1 month between each session. The primary outcomes were changes in vulva texture and pigmentation. Treatment was evaluated using images of the patients. Baseline and posttreatment images were collected and evaluated using a scoring system from 0 to 3 to grade the vulvar texture and pigmentation changes. In addition, patients rated their degree of vaginal rejuvenation after the treatment using a scoring system from 0 to 3. Results: Fractional CO2 laser treatment effectively and significantly increased vulvar texture and decreased vulvar pigmentation after three sessions (p < 0.05). Patients also self-reported noticeable improvement. There were no adverse reactions during the treatment and follow-up. Conclusions: Fractional CO2 laser treatment is a safe and effective method for vulvar rejuvenation in women.

Cell autocloning as a pathway to their real rejuvenation.

The article gives a brief description of geroprotection and rejuvenation methods known to date, presenting their main mechanisms and limitations. To overcome the main limitations of the process of rejuvenation, it is possible to use a process called "cell autocloning." The principle of the proposed method of rejuvenation is as follows: a periodic process of autocloning of the cell nucleus is initiated in the cellular genome with the formation of one unstable daughter copy and its subsequent self-elimination. In this case, the process of cell division stops in the phase of nuclei divergence without subsequent physical separation of the cell itself. This is especially important for postmitotic cells, where the looping of the "unidirectional" line of the ontogenesis program into a "ring" will mean their transition into renewable cells. The prototype for autocloning mechanisms could be the already known ways in which cells adapt to the increasing amount of their damage over time. These are polyploidy and asymmetric cell division, relying on which it is possible to obtain a renewable process of cell nuclei division, when only the original nucleus remains as a result of division. Although this is not a simple task, there are possible pathways to its solution using approaches that can suggest modern knowledge from the field of molecular and cell biology and genetics. The realization of such a goal will require a lot of work, but the expected result justifies it.

A novel WCO-MDI reactive rejuvenation method for aged SBS modified asphalt toward sustainable asphalt pavements

The sustainable advancement and low-carbon application of asphalt pavement significantly benefit from the reutilization of waste SBS modified asphalt (SBSMA) mixtures. However, the high-efficiency rejuvenation of aged SBSMA (ASBSMA) remains a persistent challenge. In this study, a novel reactive rejuvenator was developed using waste cooking oil (WCO) and methylene diphenylene isocyanate (MDI) to restore asphalt components and reconstruct SBS structures in ASBSMA. Temperature Sweep, Fourier Transform Infrared (FTIR) spectroscopy and Differential Scanning Calorimetry (DSC) tests were conducted to investigate the rejuvenation effect and mechanism of ASBSMA. The results indicate that the incorporation of WCO restores the low temperature performance of ASBSMA, but adversely affects its high temperature performance. Conversely, the addition of MDI enhances the high temperature performance of ASBSMA, but an excessive dose of MDI has a negative impact on its low temperature performance. Chemical composition analysis shows that the rejuvenation by WCO is a physical dissolution and softening process, while MDI can react with the SBS aged products to produce long chain products with an amide structure and reconstruct the cross-linked structure of SBS. Although the temperature stability of rejuvenated SBSMA is not fully restored to its original state, it shows significant improvement compared with ASBSMA due to the reconstruction of SBS structure. This study effectively clarifies the reactive rejuvenation mechanism of ASBSMA, achieves the synergistic restoration of asphalt components and SBS structure, and contributes to high-efficient recycling of WCO and waste asphalt materials.

CellBiAge: Improved single-cell age classification using data binarization

Aging is a major risk factor for many diseases. Accurate methods for predicting age in specific cell types are essential to understand the heterogeneity of aging and to assess rejuvenation strategies. However, classifying organismal age at single-cell resolution using transcriptomics is challenging due to sparsity and noise. Here, we developed CellBiAge, a robust and easy-to-implement machine learning pipeline, to classify the age of single cells in the mouse brain using single-cell transcriptomics. We show that binarization of gene expression values for the top highly variable genes significantly improved test performance across different models, techniques, sexes, and brain regions, with potential age-related genes identified for model prediction. Additionally, we demonstrate CellBiAge's ability to capture exercise-induced rejuvenation in neural stem cells. This study provides a broadly applicable approach for robust classification of organismal age of single cells in the mouse brain, which may aid in understanding the aging process and evaluating rejuvenation methods.

Thread lifting as a modern method of non-radical face rejuvenation

Purpose of the study: The purpose of this review of scientific literature is to collect, systematize and update modern ideas about the types, methods and principles of thread lifting, taking into account the basic requirements for efficiency and safety.In this paper, we reviewed publications presented in scientific databases: Web of Science, Scopus, PubMed, Cochran library, Medline, and PubMed Central. The keywords for searching for materials were: thread lift, thread lift history, thread lift sutures, face thread lift, polylactic threads, polydioxanone threads.The earliest approach to the effective use of thread rejuvenation techniques was the use of non-absorbable threads based on polypropylene, which are still successfully used along with modern threads, among which a special place is occupied by absorbable threads and new modifications of non-absorbable ones, complemented by various fixing design features (notches, cones).A large selection of threads, techniques and principles of correction allows specialists to create an individual management plan for each patient, and continuity and synergy between the sequential use of different modifications of threads allows one to prolong the results obtained and delay or refuse more radical anti-aging surgeries on the face.At the same time, as a result of the review of the scientific literature, no published studies were found on the comparative analysis of thread and surgical methods of rejuvenation, which requires further study.