Analysis Method Research Articles

Analytical calculation of Kerr and Kerr-Ads black holes in f(R) theory

In this paper, we extend Chandrasekhar’s method of calculating rotating black holes into f(R) theory. We show that the solution with constant Ricci scalar always exists in a general f(R) gravity and derive the Kerr and Kerr-Ads metric by using the analytical mathematical method. Suppose that the spacetime is a 4-dimensional Riemannian manifold with a general stationary axisymmetric metric, we calculate Cartan’s equation of structure and derive the Einstein tensor. In order to reduce the solving difficulty, we fix the gauge freedom to transform the metric into a more symmetric form. We solve the field equations in the two cases of the Ricci scalar R=0\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$R=0$$\\end{document} and R≠0\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$R\ e 0$$\\end{document}. In the case of R=0\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$R=0$$\\end{document}, the Ernst’s equations are derived. We give the elementary solution of Ernst’s equations and show the way to obtain more solutions including Kerr metric. In the case of R≠0\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$R\ e 0$$\\end{document}, we reasonably assume that the solution to the equations consists of two parts: the first is Kerr part and the second is introduced by the Ricci scalar. Giving solution to the second part and combining the two parts, we obtain the Kerr-Ads metric. The calculations are carried out in a general f(R) theory. Furthermore, the whole solving process can be treated as a standard calculation procedure to obtain rotating black holes, which can be applied to other modified gravities.

Determine Intraday Trading Currency's Trend Framework Evidence From Machine Learning Techniques

The purpose of this study is to determine the intraday hourly trading trends of currencies using predictive modeling techniques. The study encompasses two distinct intraday time intervals of 30 minutes and 1 hour, analyzing currencies from 8 different countries. It incorporates the use of wavelets MODWT to identify trends and noise in intraday currency analysis. Three predictive models, namely Support Vector Regression, Recurrent Neural Network, and Long Short-Term Memory, are applied to relative time series data to predict intraday trading currency trends. The study reveals significant noise presence in three currencies based on MODWT analysis. Additionally, it demonstrates that deep learning techniques, such as LSTM, outperform traditional machine learning approaches in accurately predicting intraday currency trends. This study contributes substantially to the theoretical understanding of international finance and provides practical insights for real-time problem-solving in currency markets. Further, this research adds to the discourse on leveraging sophisticated analytical methods within the domain of business intelligence to enhance decision-making processes in organizations operating within dynamic and complex financial environments.

Lev Gutman – A Pioneer in Theoretical Mesoscale Meteorology

Abstract On March 5, 2023, Professor Lev Gutman would have been 100 years old. This article describes Professor Gutman’s legacy in the field of dynamic mesoscale meteorology and numerical weather prediction. Gutman developed his career as a mathematician and meteorologist in the Soviet Union, where he built a school of specialists in mesoscale meteorology during the 1950s through the 1970s. He primarily worked on analytical methods to solve complex nonlinear problems, such as the structure of sea breezes, mountain-valley circulations, and thermal convection over heated terrain. Gutman pioneered the development of theories of cumulus clouds, tornados, and other atmospheric phenomena. In the 1960s, he carried out numerous research investigations on these topics with his doctoral students and collaborators at High-Altitude Geophysical Institute in Nalchik in the northern Caucasus and later at the Siberian scientific center near Novosibirsk. Gutman compiled the results from these studies into a monograph titled “Introduction to the Nonlinear Theory of Mesoscale Meteorological Processes”, which was published in Russian in 1969, and later translated into English, Chinese, and Japanese. This monograph became a major textbook for specialists in mesoscale meteorology, remaining relevant to this day. After Prof. Gutman immigrated to Israel in 1978, his collaborations expanded to include Israeli and western scientists from Europe and the United States. Gutman did not receive the recognition he deserved due to the political realities of the time. His book and his seminal analytical solutions should still be useful for early career scientists in mesoscale meteorology and atmospheric dynamics.

Quorum Quenching in Membrane Bioreactors for Fouling Retardation: Complexity Provides Opportunities.

The occurrence of biofouling restricts the widespread application of membrane bioreactors (MBRs) in wastewater treatment. Regulation of quorum sensing (QS) is a promising approach to control biofouling in MBRs, yet the underlying mechanisms are complex and remain to be illustrated. A fundamental understanding of the relationship between QS and membrane biofouling in MBRs is lacking, which hampers the development and application of quorum quenching (QQ) techniques in MBRs (QQMBRs). While many QQ microorganisms have been isolated thus far, critical criteria for selecting desirable QQ microorganisms are still missing. Furthermore, there are inconsistent results regarding the QQ lifecycle and the effects of QQ on the physicochemical characteristics and microbial communities of the mixed liquor and biofouling assemblages in QQMBRs, which might result in unreliable and inefficient QQ applications. This review aims to comprehensively summarize timely QQ research and highlight the important yet often ignored perspectives of QQ for biofouling control in MBRs. We consider what this "information" can and cannot tell us and explore its values in addressing specific and important questions in QQMBRs. Herein, we first examine current analytical methods of QS signals and discuss the critical roles of QS in fouling-forming microorganisms in MBRs, which are the cornerstones for the development of QQ technologies. To achieve targeting QQ strategies in MBRs, we propose the substrate specificity and degradation capability of isolated QQ microorganisms and the surface area and pore structures of QQ media as the critical criteria to select desirable functional microbes and media, respectively. To validate the biofouling retardation efficiency, we further specify the QQ effects on the physicochemical properties, microbial community composition, and succession of mixed liquor and biofouling assemblages in MBRs. Finally, we provide scale-up considerations of QQMBRs in terms of the debated QQ lifecycle, practical synergistic strategies, and the potential cost savings of MBRs. This review presents the limitations of classic QS/QQ hypotheses in MBRs, advances the understanding of the role of QS/QQ in biofouling development/retardation in MBRs, and builds a bridge between the fundamental understandings and practical applications of QQ technology.

High-Speed Imaging and Quantitative Analysis of Non-Equilibrium Stochastic Processes Using Atomic-Resolution Electron Microscopy

Abstract Chemical phenomena are inherently complex and stochastic, making them difficult to fully understand using conventional ensemble-averaged analytical methods. These methods primarily capture long-lived species and common structural features, limiting the study of transient intermediates and minute structural characteristics. In contrast, single-molecule time-resolved analysis using advanced microscopy techniques, particularly transmission electron microscopy (TEM), offers high spatial and temporal resolution to observe the nonequilibrium dynamics of molecules and their assemblies. This account discusses the authors’ research on developing TEM techniques to visualize intricate and transient interactions within molecular systems, enhancing the understanding of chemical phenomena at atomic and molecular levels.

Effect of Hemodynamic Monitoring Systems on Short-Term Outcomes after Living Donor Liver Transplantation

Background and Objectives: To evaluate the effects of the pulse index continuous cardiac output and MostCare Pressure Recording Analytical Method hemodynamic monitoring systems on short-term graft and patient outcomes during living donor liver transplantation in adult patients. Materials and Methods: Overall, 163 adult patients who underwent living donor liver transplantation between January 2018 and March 2022 and met the study inclusion criteria were divided into two groups based on the hemodynamic monitoring systems used during surgery: the MostCare Pressure Recording Analytical Method group (n = 73) and the pulse index continuous cardiac output group (n = 90). The groups were compared with respect to preoperative clinicodemographic features (age, sex, body mass index, graft-to-recipient weight ratio, and Model for End-stage Liver Disease score), intraoperative clinical characteristics, and postoperative biochemical parameters (aspartate aminotransferase, alanine aminotransferase, total bilirubin, direct bilirubin, prothrombin time, international normalized ratio, and platelet count). Results: There were no significant between-group differences with respect to recipient age, sex, body mass index, graft-to-recipient weight ratio, Child, Model for End-stage Liver Disease score, ejection fraction, systolic pulmonary artery pressure, surgery time, anhepatic phase, cold ischemia time, warm ischemia time, erythrocyte suspension use, human albumin use, crystalloid use, urine output, hospital stay, and intensive care unit stay. However, there was a significant difference in fresh frozen plasma use (p < 0.001) and platelet use (p = 0.037). Conclusions: The clinical and biochemical outcomes are not significantly different between pulse index continuous cardiac output and MostCare Pressure Recording Analytical Method as hemodynamic monitoring systems in living donor liver transplantation. However, the MostCare Pressure Recording Analytical Method is more economical and minimally invasive.

Ion Mobility-Mass Spectrometry Strategies to Elucidate the Anhydrous Structure of Noncovalent Guest/Host Complexes.

Structural mass spectrometry (MS) techniques are fast and sensitive analytical methods to identify noncovalent guest/host complexation phenomena for desirable solution-phase properties. Current MS-based studies on guest/host complexes of drug and drug-like molecules are sparse, and there is limited guidance on how to interpret MS information in the context of host nanoencapsulation and inclusion. Here, we use structural MS strategies, combining energy-resolved MS (ERMS), ion mobility-MS (IM-MS), and computational modeling, to characterize 14 chemically distinct drug and drug-like compounds for their propensity to form guest/host complexes with the widely used excipient, beta-cyclodextrin (βCD). The majority (11/14) yielded a 1:1 guest/host complex, and ion mobility collision cross section (CCS) analysis provided subtle evidence of gas-phase compaction of complexes in both polarities. The three distinct dissociation channels observed in ERMS (i.e., charged βCD, charged guest, and partial guest loss) were used to direct charge-site assignments for computational modeling, and structural candidates were prioritized using helium-derived CCS measurements combined with root-mean-square distance analysis. The combined analytical information from ERMS, IM-MS, and computational modeling suggested that the majority of anhydrous complexes are inclusion complexes with βCD. Taken together, this work demonstrates a roadmap for how multiple MS-based analytical measurements can be combined to interpret the structures that guest/host complexes adopt in the absence of water.

Multirezidualna metoda za določanje ostankov fitofarmacevtskih sredstev v medu s plinsko kromatografijo sklopljeno s tandemsko masno spektrometrijo

In our laboratory we introduced and validated a new analytical method for determination of environmental pesticide residues in honey. The extraction was conducted using acetone, petroleum ether and dichlorometane. The determination was conducted using gas chromatography coupled with tandem mass spectrometry. Practical usage of method was analyses of 31 samples of Slovenian honey. 33 active substances (pesticides) were sought. The insecticide cypermethrin was the only active substance found in three samples. The active substances sought were not found in 90.3 % of the samples analysed. The risk assessment showed that no unacceptable risk is expected for consumers. The results were compared with those from the literature. We revealed that honey from Slovenia contained a lower portion of positive samples per active substance sought as in Italy, comparable as in Estonia and Spain, comparable to higher as in Poland and higher as in Egypt.

Analytical Method Development and Validation for the Simultaneous Estimation of Evinacumab Injection Formulation by RP-HPLC

This paper describes in detail the RP-HPLC method used to evaluate the content of evinacumab (EVC) in combination with evkeeza injections. The mobile phase used in the assay is a mixture of potassium dihydrogen phosphate and methanol in an isocratic ratio of 70:30 (v/v) on a column Shimpac solar C18, 150*4.6, 5μm column. The flow rate was 1.0 ml/min, isocratic. At 226 nm, the quantification and detection of EVC were done concurrently. It was discovered that the response obtained was linear over the concentration range of 75-225 μg/ml, and the estimated RT in minutes for EVC was 4.063. 1.805 μg/ml was the LOD and 6.017 μg/ml was the LOQ. For the EVC assay, precision was 0.3% RSD and accuracy was 100%.

Method validation, residue behaviour and dietary risk assessment of insecticides (cyantraniliprole, acetamiprid, flubendiamide and its metabolite, des-iodo flubendiamide) in or on broccoli using LC-MS/MS.

Residue behaviour and dietary risk assessment of cyantraniliprole, flubendiamide and acetamiprid in broccoli were carried out using the QuEChERS (quick, easy, cheap, effective, rugged and safe) technique coupled with LC-MS/MS. The QuEChERS technique was validated on parameters such as linearity, accuracy, precision, robustness, matrix effects, limit of quantification (LOQ), specificity, retention time and ion ratio as per SANTE (Directorate General for Health and Food Safety) guidelines to attest to the specificity, accuracy and precision of the analytical method in estimating insecticide residues in and on broccoli heads and cropped soil. The LOQ of the method for all three insecticides was 0.01 mg/kg. The initial deposits of cyantraniliprole, flubendiamide and acetamiprid reduced to half of its concentration in 1.873-2.354, 1.975-2.484 and 1.371-1.620 days, respectively. No residues were detected in broccoli-cropped soil at harvest time (30 days after last spray). The proposed maximum residue limits (MRLs) of 1.5, 0.5-0.9 and 2.0-3mg/kg for cyantraniliprole, flubendiamide and acetamiprid were calculated using the Organisation for Economic Co-operation and Development MRL calculator. The acute and chronic dietary risk assessment of the tested insecticides identified no appreciable dietary risk to the Indian population from the consumption of broccoli heads. The findings of no dietary risk highlight the importance of informed pesticide usage in broccoli and the proposed MRL derived from this study offers crucial guidelines for the regulatory authorities, ensuring the safety of broccoli consumption.

Geosynthetic MSE walls research and practice: past, present, and future (2023 IGS Bathurst Lecture)

The technology of geosynthetic mechanically stabilized earth (MSE) walls can help solve classical geotechnical earth retaining wall problems. It can also contribute to achieving the new required performance for infrastructures, such as reliance and sustainability. To further develop this technology, it is essential to analyze the history of its progress. This study summarizes the state-of-the-art research on the mechanical and soil interaction properties of geosynthetics, physical modeling and in situ measurements, analytical and numerical modeling, and reliability analyses by reviewing approximately 728 papers published in well-known international journals in this field and some notable conference paper contributions during the period of approximately 50 years from 1972 to 2023. The latest analytical methods, such as risk-based life cycle cost and CO2 emission assessments and damage/failure predictions, are introduced to evaluate the resilience and sustainability performance of geosynthetic MSE walls. Finally, the prospects of a seismic isolation technique with new types of geosynthetics and life cycle management using a long-term sensor for geosynthetic MSE walls are discussed.

Determination of β‐Hydroxy‐β‐Methylbutyrate Content in Food Raw Materials by High‐Performance Liquid Chromatography

ABSTRACTAn analytical method based on high‐performance liquid chromatography (HPLC) method was developed for the determination of β‐hydroxy‐β‐methylbutyrate (HMB) content in food raw materials. The quantitative detection was performed by HPLC on diode array detector mode under external standard method. The limits of quantification, calculated as 10 times the standard deviation, were 2.0 g/100 g. The average recoveries were in the range of 92.3%–101%, with relative standard deviations below 3.87%, measured at three concentration levels. This method was used to determine the purity of HMB in 10 commercially available food raw materials. The real sample analysis results showed that the content and measurement uncertainties of HMB were 77.1–81.8 g/100 g and 4.51–4.68 g/100 g respectively, which met the requirement of HMB content in the range of 77–82 g/100 g in the HMB announcement. There are no studies on the detection of HMB in food raw materials in the previous reports. The established method provided reference for the rapid quantification and purity analysis of β‐hydroxy‐β‐methylbutyrate in food raw materials.

How large is the collapsed area of ground collapse induced by tunnelling

ABSTRACT Ground collapse has frequently been reported in recent years, which poses a great threat to the safety of human beings and property. This study aims to determine the collapsing diameter and volume of tunnelling-induced ground collapse by analytical method. Firstly, assuming that the surface of collapsed block is sliding along a planar surface, and the sliding area is derived based on the limit equilibrium method. Then, the collapse volume is derived by combining with the equation of the collapse trajectory. Lastly, the proposed method is verified by a physical modelling test. The results show that (i) as the unit weight of soil, buried-depth and the radius of tunnel increase, the influencing diameter and collapse volume gradually increase; (ii) with the increase in the cohesion and friction angle of the soil, the influencing diameter and collapse volume gradually decrease. Dongquan Tunnel is taken as a case study, the influencing diameter of ground by using the proposed approach is compared with the one in practical engineering. This study provides a theoretical guideline to mark the collapse warning lines by the potential ground collapse area, which is induced by excavation of shallow-buried tunnels or underground structures.

PHARMA'S NITROSAMINE CHALLENGE: A REVIEW OF A CALL FOR VIGILANCE

Nitrosamines are potential carcinogens (cancer-causing agents) that can form in medications under specific conditions. The British Pharmacopoeia (BP) and the United States Pharmacopeia (USP) both set strict guidelines to minimize their presence. Nitrosamines form when nitrites and amines react in acidic environments, potentially occurring in the stomach or during drug production. They are classified as probable human carcinogens, meaning long-term exposure at high levels may increase cancer risk. Fortunately, nitrosamine levels in most medications are very low and unlikely to pose significant health risks. Both BP and USP have implemented a new general chapter: Nitrosamine Impurities (BP: 5.20 and USP: <1469>).These chapters outline acceptable intake limits for six specific nitrosamines and a four-step risk assessment and control strategy. They also detail testing procedures and analytical methods for detecting and measuring nitrosamines in medications. There are similarities between BP and USP as both set comparable acceptable intake limits for the same six nitrosamines. Also, both employ a similar four-step risk management approach. Moreover, both provide information on testing procedures and analytical methods. Nevertheless, there are few differences between both references. There may be slight variations in the specifics of analytical procedures (e.g., detection limits, chromatographic conditions).Regulatory agencies are actively monitoring and controlling nitrosamine levels in medications, ensuring patient safety. Both BP and USP play crucial roles in setting stringent standards and ensuring their consistent implementation across the pharmaceutical industry. Peer Review History: Received 28 March 2024; Revised 2 May 2024; Accepted 25 June; Available online 15 July 2024 Academic Editor: Dr. Nuray Arı, Ankara University, Turkiye, ari@ankara.edu.tr Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewers: Dr. Maya Shaaban Eissa Hussein, Egyptian Russian University, Egypt, maya-shaaban@hotmail.com Dr. Michael Otakhor Erhunmwunse, St. Philomena Catholic Hospital, Nigeria,dedoctor4life@yahoo.com

Evaluation of Urban Complex Utilization Based on AHP and MCDM Analysis: A Case Study of China

In the context of intensive urban development, urban complexes have emerged as crucial public spaces that address the needs of urban populations. However, current research on urban complexes is predominantly qualitative and lacks a rigorous scientific and quantitative analysis. Therefore, this study employs the analytic hierarchy process (AHP) to construct a standardized system encompassing five dimensions: spatial function, spatial perception, architectural style, surrounding environment, and energy-saving technology. The objective is to determine the weights of the indices that influence people’s use of urban complexes under the goal of “humanization”. Additionally, the study quantitatively analyzes key indices using spatial syntax and other analytical methods. Subsequently, we employ multi-criteria decision making (MCDM) analysis to examine three real-world cases in China, aiming to validate further the importance of the AHP + MCDM approach, which incorporates the TOPSIS method based on grey correlation. This methodology considers both the subjective factors of crowd evaluations of urban complex usage and the interrelationships among indicators, ensuring that the statistical calculations of the indicators remain objective and scientifically robust. The results indicate that (1) the degree of facility improvement has the greatest impact on the crowd’s use of urban complexes; (2) there is a discrepancy between the results of the TOPSIS method and the MCDM evaluation model, with the MCDM evaluation method aligning more closely with real-world scenarios; and (3) the Shanghai MOSCHINO received the highest evaluation score, while the Nanjing Central Emporium received the lowest. Finally, we discuss the experimental results and propose targeted strategies for optimizing the design of urban complexes to achieve the goal of “humanization”.

Enzyme inhibiting, antibacterial and antioxidant activities of peel, pulp and seed extracts from dau Ha Chau (Baccaurea ramiflora Lour.) fruit of Vietnam

Dau Ha Chau (DHC) was produced by selecting and growing native kinds of Baccaurea ramiflora Lour. in Vietnam, where the fruit is utilized for fresh eating not only due to its distinctive sour-sweet flavor, but also because of its vitamins and minerals for the body. With the aim to enhance the value of this fruit, the peel, pulp, and seed extracts were determined antioxidant, antibacterial and antidiabetic activities. The filtrates from ethanol extract of peel and seed powders and fruit juice from squeezing pulp were evaporated at 58°C to create the extracts. The six methods of TAC, DPPH, ABTS•+, RP, FRAP, and NO• (antioxidant activities), four bacterial strains and two enzymes (associated with type 2 diabetes mellitus) were evaluated. The results showed that the extract of DHC peel exhibited the highest antioxidant activity (EC50 value from the six methods), antibacterial activity (MIC value), enzyme inhibiting activity (IC50 value), total phenolics content and total flavonoids content, at 643.81 µg/mL, 787.42 µg/mL, 651.10 µg/mL, 718.22 µg/mL, 270.38 µg/mL, 662.44 µg/mL, 16 < MIC ≤ 32 mg/mL (against Propionibacterium acnes, Escherichia coli and Bacillus cereus), 32 < MIC ≤ 64 mg/mL (against Staphylococcus aureus), 2780.91 µg/mL (against α-amylase), 541.24 µg/mL (against α-glucosidase), 119.43 mg GAE/g and 207.17 mg QE/g, respectively, whereas the opposite was true for extract of DHC seed. DHC pulp extract came second in all analytical methods. Pearson correlation coefficients with r value (0.63-0.99) showed a reasonably strong relationship between total phenolics and flavonoids contents, and antioxidant and enzyme inhibiting activities. This is the first report on the antioxidant, antibacterial and antidiabetic activities of DHC Ha Chau fruit extracts, which could be developed for medicinal, pharmaceutical, food preservative purposes, or industrial foods in the future.

Analytical Chemistry for Study of Sulphate Levels in Dyeing Effluent: A Quantitative Approach

This research work explains the analytical aspects and possible environmental ramifications of measuring and detecting sulphate in particular in the water discharged from dyeing processes. Sulphate levels in dyeing wastewater must be estimated and managed in order to protect the environment, comply with regulations, run businesses efficiently, and uphold public confidence. Sustainable industrial practices depend on reducing sulphate levels through enhanced process controls and treatment technology. Analytical methods are used to determine the amount of sulphate in dyeing effluents, and the results are compared to the recommended level provided by standard bodies.

How Does the Religiosity and Halal Certification Influence Consumer Purchase Decision on Halal Food in Java, Indonesia

Rapid economic growth in several Muslim-majority countries that uphold Islamic values can encourage the growth of Islamic businesses and lifestyles, including Indonesia, which has the largest Muslim population in the world. Rapid economic growth spread to the non-Muslim consumer segment. Java Island is one of the islands in Indonesia with the largest adherents of Islam. This research aims to find out how religiosity and halal certification can influence halal awareness and purchase intention which can increase consumer purchase decision for halal food products. It's using 100 respondents spread across Java Island. The analytical method used is Partial Least Squares – Structural Equation Modeling (PLS-SEM). The research results show that religiosity and halal certification have a positive and significant effect on halal awareness and purchase intention. Then halal awareness and consumer purchasing intentions have a positive and significant influence on halal food purchasing decisions on the island of Java, Indonesia.

Digital Marketing And City Branding Analysis Of Tourist Visits: Trust As A Mediation Variable

This study aims to test how big the analysis of digital marketing and city branding is on tourist visits to the Thousand Rumah Gadang tourist attraction in Solok Selatan with trust as the intervening variable. Methods of data collection through surveys and distributing questionnaires, with a sample of 100 respondents. The analytical method used is SEM analysis using smartpls. The research results obtained based on hypothesis testing obtained that there is a significant effect of digital marketing on trust. There is a significant influence of city branding on trust. There is a significant influence of digital marketing on tourist visits. There is a significant influence of city branding on tourist visits. There is a significant influence of trust on tourist visits. Trust mediates digital marketing relationships with tourist visits. Trust mediates the relationship between city branding and tourist visits.

Deciphering the benefits and intensity levels of primary metabolites from Allium macrostemon Bunge and Allium chinense G. Don

BackgroundAllii Macrostemonis Bulbus is also named Xiebai in China. It is an edible vegetable, and also a famous herb for treating coronary heart disease. Allium chinense G. Don (ACGD) and Allium macrostemon Bunge (AMB) are it botanical sources. The aim of this study was to explore the cardioprotective effects, and decipher the visual spatial distribution and absolute content of primary metabolites derived from these two herbs.MethodsH9c2 cells were used to perform the hypoxia-reoxygenation (H/R)-induced myocardial injury model. Their protective effects were evaluated by apoptosis levels. Furthermore, matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry imaging approach (MALDI-TOF MSI) was carried out to present the spatial location of primary metabolites including fatty acids, amino acids, carotenoids, and vitamins in these two Allium herbs. Multiple analytical methods were applied to perform quantitative analysis of these primary metabolites in AMB and ACGD bulbs by liquid chromatography tandem mass spectrometry (LC–MS).ResultsFirst, AMB and ACGD extracts both could increase the cell viability in H9c2 cells, and attenuate H/R-induced injury. They markedly decreased apoptosis, accompanied by activating the BCL-2/BAX pathway. Further, MALDI-TOF MSI-based relative quantification results showed several amino acids, fatty acids, carotenoids, and vitamins were largely rich in the tunics and outside scales of fresh bulbs, while some primary metabolites were abundant in their developing flower buds. Absolute quantification results displayed total contents of amino acids in ACGD bulbs were higher than those in AMB, while total contents of fatty acids and vitamins provides opposite trends in these two Allium herbs. The total contents of carotenoids and trace elements showed no significant differences between AMB and ACGD samples.ConclusionsThis study would be helpful to understand the myocardial injury protection effects of these two Allium herbs, and the spatial accumulation and quantitative content levels of their main nutrients.Graphical