Few studies have been conducted on the precision estimation of weighted total least squares (WTLS) by using the approximate function probability distribution method. And the existing Monte Carlo method needs to simulate a lot, the amount of calculation is large and the results obtained are uncertain. In order to further improve the total least squares precision estimation theory, this paper introduces the Jackknife method into Geomatics data processing. Combining the Jackknife method and WTLS method, the delete-1 Jackknife method and delete-d Jackknife method are proposed. The biases and standard deviations or covariance of parameter estimations are calculated by these proposed methods. And the specific steps of the precision estimation of these two methods are given. Applying these methods to the linear regression model and the coordinate transformation model, and comparing with the approximate function method and the Monte Carlo method, we can see that the Jackknife methods for precision estimation can obtain more stable and reasonable precision results and are very adaptive. In order to get more reasonable precision results, the Jackknife method does spend much more time over total least squares when the amount of the observed data is large. But compared with Monte Carlo method, it can reduce the amount of calculation and improve the computational efficiency. The method in this paper could provide an idea for further study on the precision estimation for total least squares.