Test Methodology Research Articles

ВОСТРЕБОВАННОСТЬ СРЕДНЕПОЗДНИХ СОРТОВ ЯРОВОЙ МЯГКОЙ ПШЕНИЦЫ В УСЛОВИЯХ ЗАУРАЛЬЯ

The objective of the study is to determine the characteristics and advantages of mid-late varieties for sustainable harvesting of food grain in the region based on long-term variety testing. Objectives: to study the economically valuable indicators of spring wheat varieties and to identify the advantages of late-ripening varieties. The object of the study is mid-late ripening spring soft wheat varieties, including the Raduga variety bred by the Kurgan Research Institute of Agriculture. Predecessors are fallow and provo-cative backgrounds: sowing on stubble and late sowing date. Observations and assessments are carried out in accordance with the Methodology of state variety testing. The studies were carried out at the Kurgan Research Institute of Agriculture – a branch of the Ural FRC of the Ural Branch of the RAS. Mid-late varieties, when sown at different times, excluding extremely late ones, showed an advantage in a number of economically valuable traits. The mid-late Raduga variety allows obtaining consistently high indicators in various weather conditions. For the period 2017–2023, with a yield of 1.22–5.53 t/ha, the excess over the Uralosibirskaya standard was 0.24 t/ha, with annual fluctuations from 0.08 to 0.53 t/ha. In arid conditions of 2020–2021 it was 0.15–0.20 t/ha. The variety responds to fertilizers and the use of fungicides. The grain quality met the GOST requirements for valuable wheat or a good filler. The gluten content in the grain, depending on the conditions, ranges from 23 to 37 %, protein – 14.7–15.7 % (2021–2023). Raduga, thanks to its genetically determined resistance to diseases, exceeded the standard in yield in three sowing pe¬riods by an average of 1.27 t/ha during the years of rust epiphytotics, demonstrating a resistance of 1.5 points versus 5 points for the standard.Against the background of mass germination of grain on the root in 2023, the percentage of sprouted grains in Raduga was 14.5 versus 40 % in the Uralosibirskaya standard. It is included in the list of breeding achievements for 7 and 9 regions of Russia.

Assessment of Vulnerabilities in Student Records Web-Based Systems for Public and Private Higher Learning Institutions in Tanzania

In spite that HLIs in Tanzania use web-based systems for managing, storing and processing of HLIs information and data such as website contents, academic results and financial records. The HLIs web-based system have been compromised by attackers due to presence of vulnerabilities. The main objective of this study is to assess the vulnerabilities of Students Records Web-based Systems (SRWBS) for private and public Higher Learning Institutions (HLIs) in Tanzania using black-box testing methodology by employing two automatic vulnerability scanners namely OWASP ZAP (Open Webs Application Security Project Zed Attack Proxy; open-source tool) and Acunetix (proprietary tool). This study assesses the vulnerability of SRWBS for 3 private HLIs and 5 public HLIs in Tanzania. The results reveal the total of 29 vulnerabilities which include but are not limited to Broken Authentication and Session Management, Broken Access Control, Security Misconfiguration, Sensitive Data Exposure, Vulnerable JS (Java Script) Libraries, CSRF (Cros Site Request Forgery), Using Components with Known Vulnerabilities, XSS (Cross Site Script), DOM (Document Object Model) based XSS and Reflected XSS. SRWBS of public HLIs were found more vulnerable by average 44.2% than the SRWBS of private HLIs which were vulnerable by average of 37%. Based on these results, this study provides some recommendations for mitigating vulnerabilities and improving the security of SRWBS for private and public HLIs in Tanzania.

Rethinking Human Factors in Obesity: Development of Simulation and Physical Test Models of Human Soft Tissue to Study Autoinjector Activation Performance.

Activation against a hard surface according to ISO 11608-1 is not always representative of device use on a soft injection site. A softer injection site - which is an anthropometric property found in obese patients - presents a distinct viscoelastic property which can lead to greater autoinjector activation forces that are not captured in standardized activation testing methodology. Soft tissue simulation and physical testing were developed at SHL to advance the development of autoinjectors, allowing for rigorous testing and challenging these in scenarios involving even the softest injection sites.

Exploration of Eye-Tracking Methodologies in Usability Testing of Digital Health Technology: A Rapid Review.

Eye-tracking is deemed a promising methodology for usability evaluation studies in healthcare, however clear theoretical guidance and practice remains lacking. A rapid review was performed on current use of eye tracking as a usability evaluation method on digital health technologies in the period of 2019 to 2024. Usability evaluation studies were included when they described a digital health technology intervention in which eye-tracking technologies were applied. To gain insight into how eye-tracking technologies contributed to measuring digital health technologies' usability, data was extracted on the use of eye-tracking for usability and key study findings. Seventeen papers were included in the review. Findings show that eye-tracking is frequently combined with other usability evaluation methods, with high methodological diversity, to test the usability of DHT. Future research is needed to enhance understanding of the effectiveness of eye-tracking outcomes in DHT usability testing when combined with other usability evaluation methods in order to provide (usability) researchers theoretical guidance on its application.

Exploring joining techniques for diamond chips on metallized substrates: Micro- and nano-scale mechanical testing approach

This paper aims to comprehensively evaluate the shear strength of various junctions between diamond chips and double copper bonded ceramic substrates. The study involves several stages, beginning with the deposition of Ti/Ni/Ag metallization system on diamond chips using a chemical vapor deposition (CVD) process. These metallization systems are selected to determine their suitability as ohmic contacts on diamond. In parallel, Ni/Au and Ni/Ag layers are electroplated onto the thick copper metallization of the ceramic substrate. Following these depositions, junctions are created for both Cu/Cu and C/Cu assemblies using different techniques: reflow process for AuGe solder joints, reflow-diffusion process for Ag-In joints, and a thermomechanical process for Ag nanoparticle joints. To assess the effectiveness of these techniques, the shear strength of the junctions is measured using a micro-shear test, which is analogous to a classical micro-scratching test. Additionally, the mechanical behaviors of the layers adjacent to the assemblies are analyzed through nano-indentation tests. Finally, the fracture surfaces are examined using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) spectrometry to identify the microstructure and damage modes. The work described in the paper exhibits several innovative aspects. It introduces the novel combination of Ti/Ni/Ag metallization system on diamond chips, evaluated for its suitability as ohmic contacts, potentially leading to improved electrical and thermal performance in diamond substrate applications. The study employs diverse junction creation techniques, such as reflow for AuGe solder joints, reflow-diffusion for Ag-In joints, and a thermomechanical process for Ag nanoparticle joints, allowing for a thorough comparison and identification of the most effective method for different scenarios. We have also introduced an integrated testing methodology, which combines micro-shear tests and nano-indentation tests and provides a robust framework for assessing both the shear strength and mechanical properties of the junctions and adjacent layers, enhancing the reliability and depth of the evaluation. The specific focus on metallization system and junctions suitable for diamond, known for its exceptional thermal and electrical properties, positions this work as highly relevant for advanced electronic and thermal management applications.

Active Diagnostic Experimentation on Wind Turbine Blades with Vibration Measurements and Analysis

Abstract This paper deals with the key operational problems of wind turbosets, especially offshore, where vibrations are generated by rotor blades, as a consequence of erosive wear or icing. The primary causes of the imbalance of wind turbine rotors have been characterised, the observable symptoms of which include various forms of vibrations, transmitted from the turbine wheel to the bearing nodes of the power train components. Their identification was the result of an active diagnostic experiment, which actually entered the aerodynamic-mass imbalance of a turbine rotor into a wind power train, built as a small scale model. The recording of the observed monitoring parameters (vibration, aerodynamic, mechanical and electrical) made it possible to determine a set of symptoms (syndrome) of the deteriorated (entered) dynamic state of the entire wind turboset. This provides the basis for positive verification of the assumed concept and methodology of diagnostic testing, the constructed laboratory station and the measuring equipment used. For this reason, testing continued, taking into account the known and recognisable faults that most often occur during the operation of offshore wind turbosets. Transferring the results of this type of model research to full-size, real objects makes it possible to detect secondary (fatigue) damage to the elements transmitting torque from the wind turbine rotor to the generator early, especially the thrust bearings or gear wheel teeth.

False Discovery Rate Control for Lesion-Symptom Mapping With Heterogeneous Data via Weighted p-Values.

Lesion-symptom mapping studies provide insight into what areas of the brain are involved in different aspects of cognition. This is commonly done via behavioral testing in patients with a naturally occurring brain injury or lesions (e.g., strokes or brain tumors). This results in high-dimensional observational data where lesion status (present/absent) is nonuniformly distributed, with some voxels having lesions in very few (or no) subjects. In this situation, mass univariate hypothesis tests have severe power heterogeneity where many tests are known a priori to have little to no power. Recent advancements in multiple testing methodologies allow researchers to weigh hypotheses according to side information (e.g., information on power heterogeneity). In this paper, we propose the use of p-value weighting for voxel-based lesion-symptom mapping studies. The weights are created using the distribution of lesion status and spatial information to estimate different non-null prior probabilities for each hypothesis test through some common approaches. We provide a monotone minimum weight criterion, which requires minimum a priori power information. Our methods are demonstrated on dependent simulated data and an aphasia study investigating which regions of the brain are associated with the severity of language impairment among stroke survivors. The results demonstrate that the proposed methods have robust error control and can increase power. Further, we showcase how weights can be used to identify regions that are inconclusive due to lack ofpower.

Phytochemical Testing Methodologies and Principles for Preliminary Screening/ Qualitative Testing

Plants are basically biosynthetic factories that contain various pathways that produce phytochemicals with the help of different parts of the cell while specialized cells or tissues hoard and use them as needed. Human population has used this to their advantage for centuries. Aims: This review article aims to provide a comprehensive guide to qualitative phytochemical tests, focusing on their principles, procedures, and interpretation of results. Scope of Review: This review covers qualitative tests for major phytochemical groups, including alkaloids, flavonoids, tannins, saponins, and phenols. Methodology: Various qualitative tests are compiled and described, detailing the chemical principles underpinning each test and the expected outcomes for the identification of phytochemicals. Summary of Findings: Qualitative phytochemical tests are highlighted for their simplicity, cost-effectiveness, and accessibility, making them valuable tools, particularly in resource-limited settings. These tests provide baseline information on phytochemical profiles and serve as initial screening tools that can lead to more detailed studies. Conclusion: While advanced analytical techniques exist, qualitative methods remain essential for initial phytochemical screening due to their practical advantages. This review emphasizes the importance of these classical techniques in both research and practical applications.

Divergent credit risk responses to local government debt among Chinese banks: a stress test analysis

ABSTRACT This study explores the heterogeneous impact of local government debt on credit risk across ‎state-owned and urban and rural commercial banks in China, through the analysis of stress test ‎methodology and quarter-based data in the period 2014–2021. It found that if the scale ‎of local government debt expands, the Non-Performing Loan (NPL) rates will reduce in state-owned commercial banks, ‎while the rural and urban commercial banks will increase. In the case of further decay in the scale of local government debt and macroeconomic indicators, there would be more credit risk in a heterogeneous manner among different-sized commercial banks. Medium-sized urban commercial banks have the smallest rise in non-performing loan rates due to the higher individual and corporate credit risks they face. Moreover, the study finds the resiliency of the risks weak and poor preparation for loan impairment in covering the potential loan losses in a pressured scenario compared to their counterpart urban and state-owned rural commercial banks. The findings suggest that rural commercial banks need to improve their risk ‎resilience to cope with extreme shocks.

ACPSEM position paper: recommendations for a digital general X-ray quality assurance program

This guideline has been prepared by the ACPSEM to provide a standardised quality assurance program to be used within General X-ray imaging environments. The guideline includes the responsibilities of various multidisciplinary team members within medical imaging facilities. It must be noted that the listed tests and testing frequencies are not intended to replace or become regulatory requirements. Implementing a quality assurance program as outlined in this position paper is there to ensure best practice for imaging facilities by providing a framework to establish and monitor correct equipment performance. The current document has been produced through an extensive review of current international practices and local experience within the Australasian healthcare environment. Due to the constant evolution of digital radiographic equipment, there is no current consensus in international quality assurance guidelines as they continue to be adapted and updated. This document describes the current state of the use of digital General X-ray equipment in the Australasian environment and provides recommendations of test procedures that may be best suited for the current medical imaging climate in Australasia. Due to the everchanging developments in the medical imaging environment and the ability of new technologies to perform more complex tasks it is believed that in the future this document will be further reviewed in the hopes of producing a more globally agreed upon standard quality assurance program. Any such adjustments that are deemed to be necessary to Version 1.0 of this document will be provided in electronic format on the ACPSEM website with a notification to all parties involved in the use of digital General X-ray equipment. This guideline does not provide detailed methodologies for all the quality control tests recommended as it is it is expected that the professionals implementing aspects of this quality assurance program have the working knowledge and access to appropriate resources to develop testing methodologies appropriate for their local imaging environment.

Advancing precision agriculture: domain-specific augmentations and robustness testing for convolutional neural networks in precision spraying evaluation

AbstractModern agriculture relies heavily on the precise application of chemicals such as fertilisers, herbicides, and pesticides, which directly affect both crop yield and environmental footprint. Therefore, it is crucial to assess the accuracy of precision sprayers regarding the spatial location of spray deposits. However, there is currently no fully automated evaluation method for this. In this study, we collected a novel dataset from a precision spot spraying system to enable us to classify and detect spray deposits on target weeds and non-target crops. We employed multiple deep convolutional backbones for this task; subsequently, we have proposed a robustness testing methodology for evaluation purposes. We experimented with two novel data augmentation techniques: subtraction and thresholding which enhanced the classification accuracy and robustness of the developed models. On average, across nine different tests and four distinct convolutional neural networks, subtraction improves robustness by 50.83%, and thresholding increases by 42.26% from a baseline. Additionally, we have presented the results from a novel weakly supervised object detection task using our dataset, establishing a baseline Intersection over Union score of 42.78%. Our proposed pipeline includes an explainable artificial intelligence stage and provides insights not only into the spatial location of the spray deposits but also into the specific filtering methods within that spatial location utilised for classification.

Updated national guidelines for spirometry. Part 3. Conducting and evaluating the results of bronchodilatation test. Features of spirometry in children

In the third part of the article, the bronchodilation tests methodology was considered: indications, contraindications, test procedure, interpretation. The methods of the bronchodilatory response assessing were considered, the differences between modern Russian recommendations and ERS/ATS interpretive strategies the bronchodilatory response assessing were shown. The specifics of the test procedure and evaluation of bronchodilation tests in children were described separately

A Review of State of the Art for Accelerated Testing in Fluid Power Pitch Systems

Failures in hydraulic systems in offshore wind turbines represent an enormous challenge for manufacturers and operators, as the pitch system statistically is one of the subsystems contributing the most to the downtime of the turbines, which is the case for both electrical and hydraulic pitch systems. However, the complex failure mechanisms of the various different hydraulic components mean that, typically, the critical components of hydraulic systems must be tested to better understand the failure mechanisms. Nonetheless, conventional testing procedures are lengthy and costly. Accelerated testing plays a critical role as it can mimic hydraulic system failure mechanisms in a shorter period. However, the lack of standardized test methods and detailed knowledge about the failure-accelerating effects complicates the process. Therefore, this paper offers a comprehensive examination of approaches applicable to conducting accelerated tests on hydraulic systems. It identifies and discusses five primary component types or sub-components related to the acceleration of testing in hydraulic systems: pumps, cylinders, seals, valves, and hoses. Each section references studies that delve into accelerated testing methodologies for these individual components. Furthermore, within each component, a concise overview of the current techniques is provided, followed by a discussion and summary based on the state of the art.

Comparison of sampling techniques and diagnostic tests for Cryptosporidium serpentis in eastern indigo snakes (Drymarchon couperi).

The purpose of this study was to compare various sampling techniques and commercially available diagnostic tests for Cryptosporidium serpentis. A colony of 80 eastern indigo snakes (Drymarchon couperi) in human care was screened for the presence of C serpentis using endoscopic gastric mucosal biopsies for histologic and molecular analyses. At the time of endoscopic examination and biopsy, a cloacal swab, gastric swab, and gastric lavage sample were also collected. A C serpentis-specific probe hybridization quantitative PCR (qPCR) was performed on each sample. The gastric lavage sample was divided equally for direct microscopy, acid-fast stain, rapid qualitative immunochromatographic assay, direct fluorescent antibody, and 5 different PCR analyses. If a fecal sample was available at the time of endoscopic evaluation, it was also evaluated for Cryptosporidium oocysts by direct microscopy and acid-fast staining. When comparing test results to histologic analyses, the sensitivity of the probe hybridization qPCR of gastric biopsy, gastric lavage, and gastric swab was 100% while the cloacal swab was 72%. When gastric lavage tests were compared, qPCRs outperformed the other tests. Endoscopic biopsy for histologic and qPCR analyses is recommended for disease diagnosis, while gastric lavage or gastric swab samples for qPCR analysis are as sensitive as endoscopic biopsy for screening for the pathogen but cannot diagnose disease. The results from this study allow the veterinary practitioner to select the most appropriate sample and testing methodology when evaluating an ophidian patient for gastric cryptosporidiosis.

Hydrogen breath test as a diagnostic tool

Introduction and Purpose: Hydrogen breath tests serve the crucial purpose of evaluating the absorption of nutrients in the small intestine, particularly when carbohydrates are not properly absorbed, leading to bacterial overgrowth. These tests are non-invasive, cost-effective, and widely accessible diagnostic tools used to assess various gastrointestinal disorders. The objective of this review is to evaluate the effectiveness and methodology of Hydrogen breath testing (HBT). State of knowledge: The disposal of hydrogen gas is essential for maintaining efficient microbial fermentation processes in the gut. Hydrogenotrophic microbes, including acetogens, methanogenic archaea, and sulfate-reducing bacteria, are responsible for this process. Hydrogen breath tests, conducted with substrates like glucose and lactulose, aid in diagnosing conditions such as small intestinal bacterial overgrowth (SIBO), lactose and fructose intolerance, and other carbohydrate absorption disorders. These tests generate a large volume of data, which can be analyzed using data mining techniques to uncover new hypotheses. Conclusions: Hydrogen breath tests, using various substrates, are effective in diagnosing gastrointestinal disorders such as SIBO and carbohydrate malabsorption. They offer valuable insights into microbial processes in the gut and can inform individualized treatment strategies. However, careful consideration of contraindications and proper test administration protocols is essential for accurate diagnosis and interpretation of results.

Quality Control Testing of Chloramphenicol Eye Drops

Background and aims. Choramphenicol is one of important therapeutic options for bacterial conjunctivitis. Its eye drop formulation must possess standards of good quality and sterility. The drug is very sensitive to light and temperature and must be stored between 2 and 8 ᵒ C. This study included testing two of the marketed eye-drops formulations of chloramphenicol of two different origins to ensure quality and efficacy of the drugs. Methods. The test methodology followed same pharmacopeial tests to evaluate both physical and chemical aspects of the drugs tested. Identification tests and assay were carried out. Results. Both products passed the identification tests however, there were different findings and the product from Pakistani company failed to comply with BP limits for the pH range and assay. Conclusion. It is of great importance to check on some drugs to see if affected during transport and distribution by testing samples collected randomly from pharmacies.

In-beam performance of a Resistive Plate Chamber operated with eco-friendly gas mixtures

ALICE (A Large Ion Collider Experiment) studies the Quark-Gluon Plasma (QGP): a deconfined state of nuclear matter obtained in ultra-relativistic heavy-ion collisions. One of the key probes for QGP characterization is the study of quarkonia and open heavy flavor production, of which ALICE exploits the muonic decay. In particular, a set of Resistive Plate Chambers (RPCs), placed in the forward rapidity region of the ALICE detector, is used for muon identification purposes.The correct operation of these detectors is ensured by the choice of the proper gas mixture. Currently they are operated with a mixture of C2H2F4, i-C4H10 and SF6 but, starting from 2017, new EU regulations have enforced a progressive phase-out of C2H2F4 because of its large Global Warming Potential (GWP), which is making it difficult and costly to purchase. Moreover, CERN asked LHC experiments to reduce greenhouse gases emissions, to which RPC operation contributes significantly.A possible candidate for C2H2F4 replacement is the C3H2F4 (diluted with other gases, such as CO2), which has been extensively tested using cosmic muons. Promising gas mixtures have been devised; the next crucial steps are the detailed in-beam characterization of such mixtures as well as the study of their performance under increasing irradiation levels.This contribution will describe the methodology and results of beam tests carried out at the CERN Gamma Irradiation Facility (equipped with a high activity 137Cs source and muon beam) with an ALICE-like RPC prototype, operated with several mixtures with varying proportions of CO2, C3H2F4, i-C4H10 and SF6 . Absorbed currents, efficiencies, prompt charges, cluster sizes, time resolutions and rate capabilities will be presented, both from digitized (for detailed shape and charge analysis) and discriminated (using the same front-end electronics as employed in ALICE) signals.

Environmentally friendly fire retardant natural fibre composites: A review

There is a growing drive in replacing conventional non-renewable fibres such as glass and carbon reinforced composites with more sustainable and renewable reinforcements such as flax, hemp, jute in biobased composites in key industry sectors such as automotive, marine, building and construction motivated by lower carbon footprint and sustainability. Despite this drive, flammability characteristics of these sustainable biobased composites are not fully understood. Through an up-to-date review, this article meticulously discusses the theme of bio-based and eco-friendly flame retardants (FRs), delving into their intricate mechanisms, flammability testing methodologies, and emerging research trends. It underscores the pivotal necessity of developing tailor-made eco-friendly FRs customised for a diverse range of materials to bolster fire safety in sustainable material applications. Furthermore, it illuminates the limitations associated with prevalent methods for assessing flammability and advocates for advancements in dynamic testing and multi-scale analyses to more accurately simulate real-world fire scenarios. This review also highlights the key characteristics of natural plant fibres and their composites critical for the development of sustainable and fire safe materials for key applications areas. Additionally, it highlights the diverse spectrum of strategies employed in the realm of flame-retardant materials research, emphasizing a pronounced shift towards eco-friendly alternatives, innovative coatings, and the ongoing exploration of synthetic biopolymers, nanocomposites, and fibres in the pursuit of heightened fire safety. Amidst the inherent challenges, this comprehensive review unequivocally underscores the pivotal role of interdisciplinary research collaboration in driving forward fire safety within the domain of sustainable materials.

Ensuring Transfusion Safety: Screening Blood Donors for Human Parvovirus B19.

Ensuring the safety of blood and blood products is a vital aspect of healthcare. The potential for transmission of pathogens through blood and blood products makes transfusion safety a significant concern. Despite advancements in testing methodologies, donated blood products still pose a risk for infection transmission. Human parvovirus B19 (B19V) is a small, single-stranded, non-enveloped DNA virus transmissible parenterally by blood transfusion. B19V causes a wide range of clinical manifestations, which is generally harmless in healthy individuals. B19V infection may cause severe complications, such as aplastic crises, as it affects erythrocyte progenitor cells in individuals with increased erythrocyte turnover. Additionally, B19V can be transmitted from pregnant women to their foetus, potentially causing hydrops fetalis and foetal death. The potential for transmission through blood and blood products makes B19V a significant concern for transfusion safety. In response to the growing recognition of B19V's impact on transfusion safety, various international health organisations have introduced guidelines to minimise its transmission through blood and plasma products. However, the implementation of these guidelines varies globally, with some regions, such as India, still lacking formal protocols for B19V monitoring. This review article explores the existing methodologies for screening blood donors for B19V, assesses the associated transfusion risks, and considers the implications for public health and clinical practice. By emphasising advancements in diagnostic techniques and the challenges of their implementation, this article provides a comprehensive overview of efforts to reduce the transmission of B19V through blood transfusions, thereby ensuring safer blood supplies and improved patient outcomes.

Diagnostic Strategies in the Era of Monkeypox Resurgence: A Comprehensive Analysis.

The resurgence of monkeypox (Mpox), an orthopoxvirus infection closely related to smallpox, presents a significant global health challenge. This study presents a comprehensive overview of Mpox, focusing on its clinical manifestations, diagnostic strategies, and testing methodologies. A thorough review of the literature and available data on Mpox, emphasizing diagnostic assays, clinical indicators, and laboratory testing, constitutes the core of this analysis. The study involves insights from Mpox patients and healthcare professionals engaged in its diagnosis and management. Contextualizing the research within the global spread of Mpox addresses the complexities associated with the diagnosis of the disease. The findings illuminate diverse Mpox diagnostic techniques, encompassing viral culture, immunological methods, serology,quantitative polymerase chain reaction (qPCR), electron microscopy, and advanced technologies such as artificial intelligence (AI) and the GeneXpert system. qPCR is highlighted as the benchmark for MPXV detection and quantification. These diagnostic advancements have significantly enhanced the precision and efficiency of Mpox diagnosis, facilitating prompt identification and treatment of infected individuals. The study underscores the critical importance of accurate and timely diagnosis, proper handling and transportation of clinical specimens, and the imperative for point-of-care (POC) testing to control the global spread of Mpox.