Scientific Research Research Articles

Exploring marine-based compounds as cross-linkers to improve the biocompatibility and sustainability of chitosan-based hydrogels

Chitosan is one of the most promising natural polymers, its fundamental scientific research is growing uninterruptedly and has been applied in a wide and varied range of domains, including biomedical and water treatment applications. Therefore, the search and implementation of non-synthetic and non-toxic cross-linkers for chitosan-based hydrogels is crucial for the development of more sustainable and biocompatible materials. Herein, an alternative approach has been developed to explore and exploit methanolic and aqueous extracts from five red seaweed species as covalent cross-linkers for chitosan-based hydrogels. The formation of a gel could be denoted for all extracts, whereas the protein-rich methanolic extractions afforded instantaneous gel-forming ability and greater stiffness and stability. The obtained hydrogels present large porous system with high degrees of swelling up to ca. 3000 %, and were successfully applied as dye adsorbent to remove industrial dye methyl orange withing a circular process with adsorption capacities of 728.46 ± 66.17 mg/g. Furthermore, cytotoxicity and cell-adhesion studies revealed the biocompatibility of the hydrogels and their potential applicability for tissue-engineering. This work demonstrated that methanolic and aqueous extracts from different red seaweed species could replace toxic cross-linkers. Furthermore, the unexpected ability of some extracts could pave the way for the development of new formulations for additive manufacturing, in particular for 3D printing approaches.

History of studying essential oil crops

The article provides a historical overview of the formation and development of the essential oil industry. The main attention is paid to the history of scientific research of essential oil and medicinal plants. Our ancestors may have instinctively selected certain herbs for healing, just as sick animals today seek out plants they wouldn't normally eat. Based on an analysis of the current state, the need has been determined and the priority tasks have been formed for a full-scale revival of essential oil production in the Russian Federation.The problem tatement: in the republics of the former USSR, a number of essential oil plants were cultivated: coriander, lavender, essential oil rose, sage and others. In the 1970-80s the area of cultivation of essential oil plants exceeded 200 thousand hectares. At the same time, the demand for essential oils and other essential oil products was not fully satisfied. With the collapse of the USSR, the essential oil industry declined. The growing areas for most of the essential oil crops ended up on the territory of the newly formed independent states. The cultivation and processing of essential oils in Russia decreased significantly.The goal of the research is substantiation and acquisition of knowledge about the history of emergence and development of essential oil plants, herbal medicine, active substances as a tool for developing a common problem and combining the scientific, industrial and innovative potential of all interested parties.The research methods used are source study, historical-typological and biographical methods. The research results: the opportunity to gain new knowledge about the history of the origin and distribution of essential oil plants, and the creation of a huge food base for animals and people.

Pixel-wise segmentation of cells in digitized Pap smear images

A simple and cheap way to recognize cervical cancer is using light microscopic analysis of Pap smear images. Training artificial intelligence-based systems becomes possible in this domain, e.g., to follow the European recommendation to screen negative smears to reduce false negative cases. The first step for such a process is segmenting the cells. A large and manually segmented dataset is required for this task, which can be used to train deep learning-based solutions. We describe a corresponding dataset with accurate manual segmentations for the enclosed cells. Altogether, the APACS23 (Annotated PAp smear images for Cell Segmentation 2023) dataset contains about 37 000 manually segmented cells and is separated into dedicated training and test parts, which could be used for an official benchmark of scientific investigations or a grand challenge.

Seeing is believing – The application of Three-Dimensional modelling technologies to reconstruct the final hours in the life of an ancient Egyptian Crocodile

The application of non-invasive radiography (X-ray and CT) to an ancient Egyptian crocodile mummy demonstrated a high level of corporeal preservation achieved through artificial embalming. Analysis revealed numerous anomalies within the abdomen of the crocodile which merited further investigation using digital three-dimensional modelling technologies. Improving the clarity of the CT scan data enabled the authors to identify the anomalies which included a metal fish hook and a small fish. Segmentation of the CT scan data enabled the virtual extraction of the hook from within the confines of the mummy and its replication, firstly in plastic and then in its original material, bronze.Through the scientific analysis of the mummy, the authors reconstruct the biological biography and life history attributes of the animal, and consider the human-animal relationships and religious beliefs behind its post-mortem preservation. The study explores the application of the 3D technologies applied to archaeological materials and the novel implementation of experimental techniques to reproduce the anomaly. The investigation provided the ability to enhance the study, interpretation and display of the mummy; a matter of particular relevance in the case of mummified remains where anomalies are not directly visible. The authors consider the improved accessibility to cultural artefacts made possible through the application of enhanced visualisation techniques in the heritage sector.

Failure mechanism of 18650 Li-ion batteries induced by the heating accumulation of tab

Failure analysis of lithium-ion batteries is an effective way to discovery the defects and improve the reliability of batteries. Nevertheless, defects of LIBs in manufacturing process leading to failure are easily ignored in the scientific researches. This work investigates the failure mechanism of the failed 18650 Li-ion batteries pack. The combination of non-destructive test and micro-zone characterizations rapidly locate the failure position of anode tab. Results show that the poor welding technology of anode tab induces the heating accumulation. Long-term overheating causes the peeling of active materials and the close-packed of separator pores, which finally leads to the thermal runaway of the battery.

Research on the Integration Path of Postgraduate Professional Courses into the Construction of Curriculum Ideology and Politics

The curriculum ideology and politics play an important role in cultivating students’ correct worldview, outlook on life, and values. In recent years, universities and colleges have begun to pay attention to the cultivation of postgraduates’ moral education and the construction of postgraduates’ curriculum ideology and politics. This paper takes the course “Theory of Software Engineering” of Anqing Normal University as an example, discusses the paths of integrating postgraduate professional courses into the construction of curriculum ideology and politics, and puts forward the paths of feeding back from scientific research, connecting with practice and summarizing and condensing, which are aimed at improving postgraduates’ comprehensive quality and innovation ability, and have positive significance for cultivating high-level applied scientific and technological talents who are both moral and talented.

Research integrity in the era of artificial intelligence: Challenges and responses.

The application of artificial intelligence (AI) technologies in scientific research has significantly enhanced efficiency and accuracy but also introduced new forms of academic misconduct, such as data fabrication and text plagiarism using AI algorithms. These practices jeopardize research integrity and can mislead scientific directions. This study addresses these challenges, underscoring the need for the academic community to strengthen ethical norms, enhance researcher qualifications, and establish rigorous review mechanisms. To ensure responsible and transparent research processes, we recommend the following specific key actions: Development and enforcement of comprehensive AI research integrity guidelines that include clear protocols for AI use in data analysis and publication, ensuring transparency and accountability in AI-assisted research. Implementation of mandatory AI ethics and integrity training for researchers, aimed at fostering an in-depth understanding of potential AI misuses and promoting ethical research practices. Establishment of international collaboration frameworks to facilitate the exchange of best practices and development of unified ethical standards for AI in research. Protecting research integrity is paramount for maintaining public trust in science, making these recommendations urgent for the scientific community consideration and action.

A streamlined method to generate endothelial cells from human pluripotent stem cells via transient doxycycline-inducible ETV2 activation.

The development of reliable methods for producing functional endothelial cells (ECs) is crucial for progress in vascular biology and regenerative medicine. In this study, we present a streamlined and efficient methodology for the differentiation of human induced pluripotent stem cells (iPSCs) into induced ECs (iECs) that maintain the ability to undergo vasculogenesis in vitro and in vivo using a doxycycline-inducible system for the transient expression of the ETV2 transcription factor. This approach mitigates the limitations of direct transfection methods, such as mRNA-mediated differentiation, by simplifying the protocol and enhancing reproducibility across different stem cell lines. We detail the generation of iPSCs engineered for doxycycline-induced ETV2 expression and their subsequent differentiation into iECs, achieving over 90% efficiency within four days. Through both in vitro and in vivo assays, the functionality and phenotypic stability of the derived iECs were rigorously validated. Notably, these cells exhibit key endothelial markers and capabilities, including the formation of vascular networks in a microphysiological platform in vitro and in a subcutaneous mouse model. Furthermore, our results reveal a close transcriptional and proteomic alignment between the iECs generated via our method and primary ECs, confirming the biological relevance of the differentiated cells. The high efficiency and effectiveness of our induction methodology pave the way for broader application and accessibility of iPSC-derived ECs in scientific research, offering a valuable tool for investigating endothelial biology and for the development of EC-based therapies.

Global trends and frontiers of research on Kümmell's disease: A bibliometric analysis.

The prevalence of Kümmell's disease (KD) has been increasing due to the aging population and the rise of osteoporotic vertebral compressibility fractures. As a result, there has been a growing concern about this condition. Despite the rapid advancements in its related research fields, the current research status and hotspot analysis of KD remain unclear. Therefore, our goal was to identify and analyze the global research trends on KD using bibliometric tools. All KD data were obtained from the Web of Science Core Collection. The information of research field was collected, including title, author, institutions, journals, countries, references, total citations, and years of publication for further analysis. From 1900 to 2022, a total of 195 articles and 1973 references have been published in this field, originating from 27 countries/regions and 90 journals, with China leading the contributions. The most significant institutional and author contributions come from Soochow University and Kim, HS, respectively. The journal with the highest number of published research and total citation frequency is Spine. The latest research focuses in this field include "risk factor," "osteoporotic vertebral compression fracture," "pedicle screw fixation," "percutaneous vertebroplasty," and "bone cement," and should be closely monitored. Additionally, we have conducted a comprehensive analysis of the 50 most-cited articles in KD, providing a valuable list of articles to guide clinical decision-making and future research for clinicians and researchers. In recent years, there has been a significant increase in scientific research on KD. Future research in KD is likely to focus on surgical treatment, risk factors, and complications.

Solar light assisted enhanced photocatalytic activity of smart ternary ZnO:TiO2:SnO2 nanocomposites

In the present study, the smart combinations of metal oxide semiconductors (ZnO:TiO2)1-x(SnO2)x (ZTS) with different SnO2 loadings (as x = 0.0, 0.30, 0.25, 0.20, coded as ZTS0, ZTS1, ZTS2, and ZTS3) were synthesized by employing the simple sol-gel technique. The structural, morphological, optical properties and elemental composition were analyzed by using XRD, Raman spectroscopy, FE-SEM, BET, UV–Vis spectroscopy, IC-PMS, XPS and FTIR of ZTS nanocomposite and proved the formation of smart ternary combination of nanocomposites. Systematic investigation through the characterization emphasised that the acute interfacial combination was established between metal oxide semiconductors. The crystallite size was decreased on adding the amount of SnO2 in the pristine ZTS0 nanocomposites from 23.5 nm to 10.5 nm which was confirmed by Scherrer's equation. The optical energy band gap of the obtained ZTS nanocomposites was found to vary from 3.23 to 3.34 eV on adding the amount of SnO2. The performance of the ZTS nanocomposites as photocatalyst was examined towards organic dyes and the results shows highest degradation of MB and MO dyes with ZTS3 achieving 99 % and 97.3 % degradation efficiency with 1 g/L loading dose. The plausible mechanism was suggested considering the visible light absorption spectra and band diagram resulting in supressed of the recombination rate of charge carriers. The smart combination of ZTS nanocomposites makes it an ideal material for excellent photocatalytic activity for degrading organic contaminants in presence of solar light irradiation.

The identification, evolutionary analysis, and immune roles of Rab family members in red swamp crayfish, Procambarus clarkii

The Rab GTPase constitutes the largest family of small GTPases that regulate intracellular trafficking. Different eukaryotes possess varying numbers of Rab paralogs. However, limited knowledge exists regarding the evolutionary pattern of Rab family in most major eukaryotic supergroups. This study cloned 24 Rab genes from transcriptome data of Procambarus clarkii haemocytes. The multiple sequence alignment and phylogenetic tree analysis revealed a relatively high degree of conservation for PcRab. Furthermore, PcRab exhibited similarities in motif composition with all members showing presence of G, PM, RabF, and RabSF motifs. The tertiary structure indicated that PcRab proteins mainly consisted of α-helices and β-strands, and most PcRab proteins shared similar tertiary structures, and it was indicated that they have similar protein characteristics. Protein-protein interaction prediction identified a total of 20 interacting proteins involved in vesicle trafficking, phagocytosis, and signal transduction with 193 interactions. Expression analysis showed wide expression patterns for PcRab in P. clarkii organs. Upon infection by white spot syndrome virus and Aeromonas veronii, significant induction was observed for PcRab gene expression levels, indicating their involvement in pathogen response mechanisms. The present study represents the pioneering effort in comprehensively identifying and cloning the Rab family genes in crustacean, followed by a systematic investigation into their evolutionary patterns and immune response upon pathogen infection. The results provided valuable insights for further investigation into the molecular mechanism underlying the response of P. clarkii to pathogen infection.

Composition and transport of silicon in rivers of the Bohai rim with implications for the coastal environment

The transportation of silicon (Si) by rivers to the sea plays a vital role as an external source of Si budget for coastal environments, impacting the carbon cycle in the ocean. Nevertheless, the transport of reactive silica (RSi) from small rivers to the coastal sea has been frequently disregarded in scientific investigations. This research focused on 24 rivers situated along the Bohai Sea (BS) Rim, encompassing small rivers (SR) and the largest river in the region, the Yellow River (YR), to analyze their concentrations and fluxes of dissolved silicate (DSi), biogenic silica (BSi) and other amorphous forms of Si. The findings indicated seasonal variations in DSi concentrations, with higher levels observed during the flood season. Annually, approximately 105 × 103 t DSi and 200 × 103 t BSi were transported to the BS, with SR and YR contributing equally to the total riverine BSi flux. The smaller rivers were found to increase the BSi fraction of RSi due to elevated biological fixation. The ratios of average DSi and BSi fluxes to the river watershed area of SR were 3.5 and 6 times higher, respectively, compared to those of YR. SR play a critical role in the terrestrial Si export in the BS Rim. Human activities have led to significant deviations in the Si ratios to nitrogen and phosphorus in these rivers from the Redfield-Brzezinski ratio. This discrepancy could impact the phytoplankton community, primary production, and the environment of the BS. The study highlights the substantial contribution of SR to coastal environments, particularly in semi-closed marine environments like the BS.

Preparing the Next Generation of Integrative Organismal Biologists.

Pursuing cutting edge questions in organismal biology in the future will require novel approaches for training the next generation of organismal biologists, including knowledge and use of systems-type modeling combined with integrative organismal biology. We link agendas recommending changes in science education and practice across three levels: broadening the concept of organismal biology to promote modeling organisms as systems interacting with higher and lower organizational levels; enhancing undergraduate science education to improve applications of quantitative reasoning and modeling in the scientific process; and K-12 curricula based on Next-Generation Science Standards emphasizing development and use of models in the context of explanatory science, solution design, and evaluating and communicating information. Out of each of these initiatives emerges an emphasis on routine use of models as tools for hypothesis testing and prediction. The question remains, however, what is the best approach for training the next generation of organismal biology students to facilitate their understanding and use of models? We address this question by proposing new ways of teaching and learning, including the development of interactive web-based modeling modules that lower barriers for scientists approaching this new way of imagining and conducting integrative organismal biology.

How Innovation Systems Promote and Hinder Innovations in Healthcare - a Swedish Case

Healthcare organizations must remain up-to-date when healthcare systems are in constant flux. One way to meet challenges is through innovations. The aim of this study is to explore what promotes and hinders the implementation of innovation in healthcare's complex organizational environment. An innovation system was studied through qualitative interviews with nine respondents. The results show that the implementation and adoption of innovations in healthcare are complex. The complexity of the innovation determines the likelihood and speed of the adoption among healthcare professionals. Promoters such as human and financial capital were seen as critical for the sustainability of the innovation. Evaluating the innovation through scientific processes was also important to gain legitimacy and is seen as an important contribution to research. The identifying promoters and hindrances are practical implications to prompt reflection on healthcare innovations among managers and healthcare professionals.

The strange career of Millian methods in comparative social science

The strange career of Millian methods in comparative social science

A systematic first-principles investigation of the structural, electronic, mechanical, optical, and thermodynamic properties of Half-Heusler ANiX (ASc, Ti, Y, Zr, Hf; XBi, Sn) for spintronics and optoelectronics applications.

This paper is the first to look at the structural, electronic, mechanical, optical, and thermodynamic properties of the ANiX (ASc, Ti, Y, Zr, Hf; XBi, Sn) half-Heusler (HH) using DFT based first principles method. The lattice parameters that we have calculated are very similar to those obtained in prior investigations with theoretical and experimental data. The positive phonon dispersion curve confirm the dynamical stability of ANiX (ASc, Ti, Y, Zr, Hf; XBi, Sn). The electronic band structure and DOS confirmed that the studied materials ANiX (ASc, Ti, Y, Zr, Hf; XBi, Sn) are direct band gap semiconductors. The investigation also determined significant constants, including dielectric function, absorption, conductivity, reflectivity, refractive index, and loss function. These optical observations unveiled our compounds potential utilization in various electronic and optoelectronic device applications. The elastic constants were used to fulfill the Born criteria, confirming the mechanical stability and ductility of the solids ANiX (ASc, Ti, Y, Zr, Hf; XBi, Sn). The calculated elastic modulus revealed that our studied compounds are elastically anisotropic. Moreover, ANiX (ASc, Ti, Y, Zr, Hf; XBi, Sn) has a very low minimum thermal conductivity (Kmin), and a low Debye temperature (θD), which indicating their appropriateness for utilization in thermal barrier coating (TBC) applications. The Helmholtz free energy (F), internal energy (E), entropy (S), and specific heat capacity (Cv) are determined by calculations derived from the phonon density of states.

Mind the Gap: A Review of Disjunctions in Coastal Marine Species.

Many coastal marine species have discontinuous distributions or genetic breakpoints throughout their geographical ranges. These spatial and genetic disjunctions occur in species that span limited to broad dispersal potential. Thus, the mechanisms that underlie these disjunctions remain speculative or incompletely known, particularly on small spatial scales where long-term historical processes are unlikely to be the only mechanism contributing to disjunction. Rather, ecological or oceanographic factors may be important. To identify key drivers of coastal disjunctions, we reviewed publications investigating spatial and genetic disjunctions in coastal marine species and visually summarized where and why they are thought to occur. The most frequently cited mechanisms implicated in causing disjunctions include historical processes, oceanographic features, heterogeneous habitat, species introductions, and limited larval dispersal capacities. However, the relative importance of each of these processes varies depending on the spatial scales investigated. Furthermore, locations associated with disjunctions for a suite of species are typically associated with multiple processes that maintain these disjunctions. This study provides a non-exhaustive synthesis of disjunctions in coastal marine species by visualizing where they occur, exploring underlying mechanisms, and investigating biases in how the scientific community studies this phenomenon.

Sample size calculation for data reliability and diagnostic performance: a go-to review

Sample size, namely the number of subjects that should be included in a study to reach the desired endpoint and statistical power, is a fundamental concept of scientific research. Indeed, sample size must be planned a priori, and tailored to the main endpoint of the study, to avoid including too many subjects, thus possibly exposing them to additional risks while also wasting time and resources, or too few subjects, failing to reach the desired purpose. We offer a simple, go-to review of methods for sample size calculation for studies concerning data reliability (repeatability/reproducibility) and diagnostic performance. For studies concerning data reliability, we considered Cohen’s κ or intraclass correlation coefficient (ICC) for hypothesis testing, estimation of Cohen’s κ or ICC, and Bland-Altman analyses. With regards to diagnostic performance, we considered accuracy or sensitivity/specificity versus reference standards, the comparison of diagnostic performances, and the comparisons of areas under the receiver operating characteristics curve. Finally, we considered the special cases of dropouts or retrospective case exclusions, multiple endpoints, lack of prior data estimates, and the selection of unusual thresholds for α and β errors. For the most frequent cases, we provide example of software freely available on the Internet.Relevance statement Sample size calculation is a fundamental factor influencing the quality of studies on repeatability/reproducibility and diagnostic performance in radiology.Key points• Sample size is a concept related to precision and statistical power.• It has ethical implications, especially when patients are exposed to risks.• Sample size should always be calculated before starting a study.• This review offers simple, go-to methods for sample size calculations.Graphical

Spatial deprivation of children with autism spectrum disorders in the arctic city

The inclusion of children with autism spectrum disorders in society is an urgent social task that, due to its complexity, requires new perspectives of scientific research for the sound regulation of inclusive processes. The problem of the study was determined by the contradiction between the social significance of the development of inclusion and the risks of including children with ASD in the urban space existing in the Arctic city. We have introduced the category of spatial deprivation of children with autism spectrum disorders into the research field, and proposed its author's interpretation. The empirical study was aimed at identifying the state of inclusion of children with ASD in the space of the Arctic city. The results of the study showed their significant spatial deprivation in the Arctic city. It manifests itself in relation to public, semi-public spaces, spaces for movement, expressed both in their insufficient accessibility and in a significant limitation of forms of development. The conclusion is made about the low social status of children with ASD in the Arctic city, as well as about the presence of obstacles to their realization of their universal needs. This situation is mainly determined by socio-psychological and organizational factors and objectifies the difficulties of developing inclusive processes.

КОРРЕЛЯЦИОННЫЙ АНАЛИЗ СИЛЫ СВЯЗИ МЕЖДУ РЕЗУЛЬТАТАМИ МОНИТОРИНГА АВАРИЙНОГО НЕФТЯНОГО ЗАГРЯЗНЕНИЯ ПОЧВ СКРИНИНГОВЫМИ МЕТОДАМИ

Major accidents at oil and gas industry enterprises related to soil contamination with petroleum products are considered. Based on the analysis of scientific research, it was determined that the existing methods for assessing the content of petroleum products in the soil have a number of significant limitations. Overcoming them requires the organization of a soil monitoring system, characterized by the possibility of rapid determination of pollutants and the allocation of clear boundaries of the permissible content of oil and petroleum products. The use of the arbitration method of infrared spectroscopy and the molecular luminescence method for estimating the content of petroleum products in the soil is experimentally justified. A correlation analysis was carried out between the measurement results obtained using the two presented methods. It was found that at low concentrations of petroleum products in soils, there is a linear dependence with a very high binding force, characterized by a Pearson correlation coefficient of 0,93. With relatively high values of the content of petroleum products in the soil and a significant spread of the experimental data obtained, it is advisable to use rank correlation. The use of the identified dependencies allows us to form a fairly complete picture of the situation in the study area associated with soil contamination with petroleum products, based on the results obtained using one of the presented screening methods. This greatly simplifies the procedure for assessing the state of the soil cover and creates prerequisites for reducing decision-making time and increasing the responsiveness to emerging emergencies.