Assessment Methodology Research Articles

Cybersecurity governance: Strengthening policy frameworks to address global cybercrime and data privacy challenges

In an increasingly interconnected world, cybercrime and data privacy challenges have escalated into critical global issues, threatening governments, organizations, and individuals alike. The proliferation of sophisticated cyberattacks, including ransomware, data breaches, and nation-state hacking, underscores the urgent need for robust cybersecurity governance. Compounding these threats are evolving regulatory landscapes and a lack of harmonized international frameworks, leaving gaps in addressing cross-border cybercrimes and ensuring data privacy. This article explores the imperative of strengthening cybersecurity policy frameworks to combat global cybercrime and safeguard sensitive data. It begins with an overview of the current cybersecurity governance landscape, highlighting gaps and inconsistencies in policy enforcement. Emphasis is placed on the integration of adaptive regulatory mechanisms, public-private partnerships, and standardized global practices to ensure a unified response to cyber threats. Key strategies discussed include the adoption of proactive risk assessment methodologies, the implementation of privacy-by-design principles, and the enhancement of international cooperation for intelligence sharing and joint cyber defense initiatives. The article also delves into case studies illustrating the effectiveness of comprehensive policy frameworks in mitigating cyber risks and fostering organizational resilience. As cyber threats continue to evolve, addressing these challenges requires a coordinated and forward-looking approach that balances innovation with security. By advancing cybersecurity governance, stakeholders can strengthen trust in digital ecosystems, safeguard privacy, and ensure the continuity of global digital operations.

Current practices of pain assessment and analgesic use in laboratory mice: A 2022 FELASA Working Group survey.

Assessing and alleviating pain in animals involved in research is critically important. However, the effective implementation of pain management depends on the knowledge and attitudes of the personnel involved. Following a Federation of European Laboratory Animal Science Associations 'Pain in Mice' working group initiative, a questionnaire to survey current practices concerning analgesic use in laboratory mice was distributed to several professional groups in the field of laboratory animal science. Besides demographic data, attitudes to pain and analgesia and sources of information and advice on pain management were assessed. Data were gathered and analysed through an e-survey provider. Most respondents (N = 222) were from Europe (90%). Analgesics were administered to murine surgical models by 92% of respondents in most cases and by 66% to all mice undergoing surgery. Most respondents used multimodal analgesic regimens (69%). For non-surgical models, 34% of respondents provided analgesics. The most commonly administered classes of analgesics were opioids (mostly buprenorphine) and non-steroidal anti-inflammatory drugs (mostly meloxicam and carprofen). A wide range of dose rates of meloxicam and carprofen was reported. Local anaesthetics were also widely used in surgical models (mostly lidocaine). Pain assessment was undertaken by most respondents (98%). In conclusion, most respondents provided analgesics to mice undergoing surgery and used analgesics in some non-surgical models. A considerable variation in the dose range used and the timing of administration of analgesics likely reflects both a lack of data and variation in pain assessment methodologies.

Developing Adverse Outcome Pathways to support radioecological risk assessment: Challenges and insights.

Environmental pollution associated with long term effects, especially in the case of ionizing radiation, poses significant risks to wildlife, necessitating a more nuanced approach to Ecological Risk Assessment (ERA). In radioecology, current methods, as outlined by the International Commission on Radiological Protection (ICRP), focus primarily on exposure and individual/population-level effects, often both suffering a lack of ecological realism due to the nature of data used, and, sidelining a big amount of critical non-individual effects such as sub-individual one like genotoxicity. This review aims to address these gaps by suggesting the integration of New Approach Methods (NAMs) and the Adverse Outcome Pathway (AOP) framework in the field of radioecology. NAMs encompass innovative techniques, such as in silico and in vitro methodologies, that can provide predictive insights without relying solely on traditional animal testing. The AOP framework, developed by the Organization for Economic Cooperation and Development (OECD), structures effects data into a sequence of causally linked events, enabling a clearer understanding of how molecular changes lead to adverse ecological outcomes. In the first section of the review, we explore the challenges of applying AOPs within radioecology, including the complexities of modelling realistic exposure scenarios, the temporal dynamics of effects, and the impacts of multiple stressors. The second section highlights the potential and the application of some NAMs within an AOP framework to contribute improving risk assessment methodologies (in species realism issue and the use of sub-individual data). This part also offers other potential solutions to increase the number of data to be used in ERA as well as their ecological realism, through the use of AOP framework with relevant biological scales and ecological endpoints still uninvestigated in such way. In conclusion, leveraging NAMs and AOPs is much valuable for bridging molecular data and ecological implications, thereby advancing regulatory practices in radioecology and ensuring more comprehensive protection of ecosystems from radiological hazards.

Development of Electronic Objective Structured Clinical Examinations (e-OSCEs) for Prosthodontic Clinical Assessment.

Objective Structured Clinical Examinations (OSCEs) serve as a reliable assessment tool for clinical and competency evaluation. Traditional OSCEs, involving live patients, present logistical challenges and evoke student anxiety. In an effort to create a comprehensive clinical series of examinations, electronic OSCEs (e-OSCEs) were developed for assessing clinical competencies in prosthodontics at a large dental school. This manuscript aims to describe e-OSCEs as a competency assessment. The e-OSCEs were implemented for the graduating classes from 2014 to 2023, covering categories of Single-unit Crowns and Fixed Partial Dentures, Implants, Removable Partial Dentures and Complete Dentures. A digital library of clinically relevant questions was developed to simulate chairside scenarios. Competence in any area was defined as scoring a 100% where questions were taken from a pool that represented the full scope of the clinical procedure. Ten-year retrospective data indicate sustained student performance over the years, with the majority achieving competence after three e-OSCE attempts. Advantages of the e-OSCE include standardised assessments, reduced examiner bias and adaptability to external disruptions in addition to fair and relevant evaluations, cost-effectiveness and test security. Evaluation of e-OSCE outcomes allows ease of analysis for curriculum enhancement and modification. The e-OSCE method of clinical assessment offers a standardised, clinically equivalent and efficient process for assessing dental students' competencies. The assessment methodology aligns with US accreditation standards and can be easily adapted by other institutions with different grading parameters for comprehensive clinical assessments. This approach provides a feasible and effective method for assessments in dental education.

Determining a point of departure for skin sensitization potency and quantitative risk assessment of fragrance ingredients using the GARDskin dose-response assay.

Potency and quantitative risk assessment are essential for determining safe concentrations for the formulation of potential skin sensitizers into consumer products. Several new approach methodologies (NAMs) for skin sensitization hazard assessment have been developed, validated, and adopted in OECD test guidelines. However, work is ongoing to develop NAMs for predicting skin sensitization potency on a quantitative scale for use as a point of departure (POD) in next-generation risk assessment (NGRA). GARDskin Dose-Response (DR) is an adaptation of the validated GARDskin assay (OECD TG 442E), and the readout of the assay is a quantitative potency prediction similar to the No Expected Sensitization Induction Level (NESIL) value (µg/cm2). The goal of this study was to evaluate the performance of the GARDskin DR assay for potency prediction of fragrance ingredients. One hundred (100) fragrance ingredients from a reference database covering varied structural reactivity domains and potency were tested in GARDskin DR. Materials tested had varied protein-binding reactivity alerts, including Schiff base, Michael addition, SN2, and acylation. Potency categories were predicted with a total accuracy of 37% and an approximate accuracy (exact match or off by 1 category) of 81%. Combining predicted weak and very weak categories increased total accuracy to 53% and approximate accuracy to 98%. The mean prediction error for the NESIL and local lymph node assay (LLNA) EC3 was 3.15- and 3.36-fold, respectively. Based on the results of this study, GARDskin DR is a promising predictor of skin sensitization potency with an applicability domain covering a wide range of fragrance ingredient reaction mechanisms, increasing the confidence in using the assay to conduct NGRA, ultimately reducing the need for animal testing.

Performance Evaluation and Integration Strategies for Solar Façades in Diverse Climates: A State-of-the-Art Review

This review article discusses the performance evaluation and integration strategies for solar façades, focusing on photovoltaic (PV) façades in diverse climatic conditions. It examines recent technology developments and methodologies for performance assessment, highlighting the potential of solar façades to enhance energy performance through on-site electricity generation. This study offers novel insights into the economic viability of façade-mounted photovoltaics, highlighting their potential in urban areas with constrained rooftop availability. Additionally, it emphasizes their distinct advantages in cold climates, such as reduced maintenance requirements and extended operational lifespans. Additionally, it addresses challenges such as technical complexity, esthetic considerations, and market awareness, emphasizing the balance between efficiency and design. Novel findings underscore the promise of advanced materials like perovskites in improving the flexibility and performance, as well as strategies to optimize BIPV integration in different climate zones. For stakeholders, this research highlights the importance of supportive policies and innovative solutions to overcome barriers, while offering researchers valuable perspectives on the advancement of solar façades toward zero-energy and zero-carbon building targets.

Methylene blue at recommended concentrations alters metabolism in early zebrafish development

Methylene blue (MB) is an antifungal agent widely used during critical stages of zebrafish development. Most guidelines recommend 0.00005% or 0.0001% of MB for embryo/larval rearing. The Organisation for Economic Co-operation and Development zebrafish embryo toxicity test guideline omits MB recommendations, leading to inconsistent MB use in zebrafish research. Because MB affects oxidative energy metabolism in vitro and in vivo, we investigate possible metabolic effects of recommended MB concentrations in developing zebrafish (1–5 days post-fertilization (dpf)). MB increases O2 consumption rate at 1 dpf, followed by an overall reduction in oxidative energy metabolism in post-hatch eleutheroembryos (4–5 dpf). Concomitantly, mitochondrial transcripts decrease in 1 and 4 dpf zebrafish. Our findings show that MB, at recommended husbandry concentrations, affects oxidative metabolism and can thus confound experiments. Since the zebrafish embryo/larval model is gaining traction as a high-throughput New Approach Methodology (NAM) for toxicity assessment, researchers should reconsider MB use.

A Practical Human-Centric Risk Management (HRM) Methodology

Various standards (e.g., ISO 27000x, ISO 31000:2018) and methodologies (e.g., NIST SP 800-53, NIST SP 800-37, NIST SP 800-161, ETSI TS 102 165-1, NISTIR 8286) are available for risk assessment. However, these standards often overlook the human element. Studies have shown that adversary profiles (AP), which detail the maturity of attackers, significantly affect vulnerability assessments and risk calculations. Similarly, the maturity of the users interacting with the Information and Communication Technologies (ICT) system in adopting security practices impacts risk calculations. In this paper, we identify and estimate the maturity of user profiles (UP) and propose an enhanced risk assessment methodology, HRM (based on ISO 27001), that incorporates the human element into the risk evaluation. Social measures, such as awareness programs, training, and behavioral interventions, alongside technical controls, are included in the Human-Centric Risk Management (HRM) risk treatment phase. These measures enhance user security hygiene and resilience, reducing risks and ensuring comprehensive security strategies in SMEs.

The role of ultrasound combined with water bath in the establishment of animal models of rat urethral stricture

Urinary tract injuries represent a significant clinical challenge, necessitating precise diagnosis and effective treatment strategies. Rat models are preferred for studying urinary tract injuries due to their size, visibility of external genitalia, and robust reproductive and growth capabilities. However, there is a lack of standardized methodologies for evaluating the endpoints of rat urinary tract injury models. This study aimed to investigate the methodology of model establishment, imaging evaluation techniques, and endpoint effectiveness. Twenty-four male Sprague-Dawley rats were randomly assigned to groups, including a blank control group and surgical groups. The surgical groups underwent urethral injury induced by recombinant transforming growth factor-β1 (TGF-β1) solution, followed by the random selection of one surgical group (n = 6) to receive treatment with mesenchymal stem cell (MSC) exosomes. High-frequency ultrasonography using a GE E10 device combined with a water bath method was employed to evaluate the urethral conditions of all rats. Ultrasonographic characteristics were scored for all surgical group rats, and an intergroup analysis was conducted between the surgical group and the control group rats. Statistical analysis was conducted using SPSS 22.0 software. Ultrasonographic assessment revealed significant differences in urethral echogenicity between the normal group and the surgical group, with noticeable changes in urethral morphology post-injury. Histopathological examination confirmed more severe urethral stenosis in the TGF group compared to the MSC group. High-frequency ultrasound could effectively differentiate between the two groups of rats. Additionally, there were significant differences in the scoring of ultrasonographic characteristics between the two groups. Urethral stricture presents a complex challenge in urology, often requiring invasive treatment modalities. The establishment of animal models plays a crucial role in understanding and addressing this condition. TGF-β1 induced fibrosis and MSC therapy represent promising avenues for urethral stricture management. High-frequency ultrasound combined with the immersion method offers a non-invasive and cost-effective approach for evaluating rat urethral stricture models. This study highlights the practicality of high-frequency ultrasound combined with the water bath method in evaluating rat urethral stricture models, as it can effectively differentiate between rats in the pure TGF-β1 induced group and those in the MSC treatment group based on urethral changes. The results underscore the importance of standardized methodologies for model assessment and the potential of non-invasive imaging techniques in preclinical research. Overall, this study contributes to advancing our understanding of urinary system injuries and holds implications for future therapeutic interventions in urology.

Postoperative accuracy quantification of corrective osteotomies: standardisation of Q3D-CT methodology

PurposeCurrently, no gold standard exists for 3D analysis of virtually planned surgery accuracy postoperatively. The aim of this study was to present a new, validated and standardised methodology for 3D postoperative assessment of surgical accuracy in patients undergoing 3D virtually planned and guided corrective osteotomies.MethodsAll patients who underwent 3D planned corrective osteotomy in 2021–2022 at our center with a postoperative CT were included. Postoperative surgical outcome was analysed with a postoperative CT and compared to the preoperative virtual surgical planning to determine achieved accuracy. Validation of the analysis was performed by evaluating the individual assessment of six experienced observers. A postoperative quantification was performed according to the proposed innovative methodology based on rotation axes of a virtual postoperative bone model aligned to the virtual preoperative bone model and virtual surgical planned bone model. To evaluate the intra-observer variability, one observer performed the assessment twice.ResultsQuantification of 13 patients according resulted in measurements with a median range (and its interquartile range) for 3D translation of: 2.43 mm (3.17), for the angle deviations: 3D rotation, 2D coronal, 2D sagittal and 2D axial were: 0.66° (1.66°), 0.74° (0.44°), 0.99° (1.27°), 2.37° (5.00°), respectively. The inter- and intraobserver reliability established with the Intraclass correlation coefficient was for all measurements excellent (> 0.76).ConclusionThe proposed 3D CT technique provides an significant more accurate and objective method for assessment of surgical outcome of a guided corrective osteotomy. The present proposed novel methodology showed excellent inter- and intra-observer reliability with clinically acceptable absolute surgical outcome measurements.

Low-impact, near real-time risk assessment for legacy IT infrastructures

In an era where cybersecurity threats are evolving at an unprecedented pace, this paper introduces a methodology for near real-time risk assessment of high-profile, high security infrastructures, where data security and operational continuity inherently limits observability. Our approach addresses the challenges of this limited observability and minimized disruption, offering a new perspective on processing and evaluating cybersecurity knowledge. We present an innovative method that leverages attack graphs and attacker behavior analysis to assess risks and vulnerabilities. Our research includes the development of an automated risk assessment mechanism, graphical security modeling, and a Markov chain-based model for attacker behavior. Our methodology utilizes a blend of direct and indirect event sources, incorporating an attacker behavioral model based on a random walk method akin to Google’s PageRank. The proof-of-concept solution calculates potential risk according to the actual threat landscape, providing a more accurate and timely assessment.

A Review of LCA Studies on Marine Alternative Fuels: Fuels, Methodology, Case Studies, and Recommendations

Life Cycle Assessment (LCA) methodology can be used to quantitatively assess the greenhouse gas emissions of low- or zero-carbon marine alternative fuels throughout their life cycle (from well to wake) and is an important basis for ensuring a green energy transition in the shipping industry. This paper first clarifies the trends and requirements of low-carbon development in shipping and introduces the major ship emission reduction technologies and evaluation methods. Next, the characteristics of various alternative marine fuels (i.e., LNG, hydrogen, methanol, ammonia, and biofuels) are comprehensively discussed and analyzed in terms of production, storage, transportation, and ship applications. In addition, this work provides a comprehensive overview of LCA methodology, including its relevant standards and assessment tools, and establishes a framework for LCA of marine alternative fuels. On this basis, a literature review of the current research on LCA of marine alternative fuels from the perspectives of carbon emissions, pollution emissions, and economics is presented. The case review covers 64 alternative-fueled ships and 12 groups of fleets operating in different countries and waters. Finally, this paper discusses the main shortcomings that exist in the current research and provides an outlook on the future development of LCA research of marine alternative fuels.

Sub-Regional Biophysical and Monetary Evaluation of Ecosystem Services: An Experimental Spatial Planning Implementation

Preserving soil is crucial for addressing the key challenges of the new millennium, like climate change and biodiversity loss. Spatial planning plays a pivotal role in stopping soil consumption and degradation, thereby safeguarding soils that provide valuable ecosystem services. With the advent of the System of Environmental-Economic Accounting by the UN, countries are developing a shared protocol for the biophysical and monetary quantification of ecosystem services. However, downscaling efforts are necessary and must be conditioned by the national context, policies, economic dynamics, and data availability. Therefore, this research proposes a soil quality assessment methodology based on its ecosystem value at the sub-regional level in northern Italy, building upon national guidelines. This study includes modeling and mapping outputs involving six ecosystem services through eight biophysical indicators and the monetary quantification of these services. Both assessments have been conducted over two time periods to highlight the impacts of land cover transformation.

Utilizing ATLS® Trauma Management Protocols: The Gold Standard for Safe and Effective Care.

Introduction: Approximately 5.8 million people die each year as a result of injuries. Traumatic injury is the leading cause of death under the age of 45 in the U.S. and worldwide. Traumatic injuries represent a significant global health burden, demanding swift and decisive intervention to mitigate morbidity and mortality. The Advanced Trauma Life Support (ATLS) course has emerged as a cornerstone in trauma management, offering healthcare providers a systematic approach to assessment, resuscitation, and definitive care. This article explores the pivotal role of ATLS protocols as the gold standard for safe and effective trauma management. ATLS protocols prioritize a structured assessment methodology, employing the ABCDE approach to rapidly identify and address life-threatening injuries. By adhering to this systematic framework, healthcare providers can promptly initiate time-critical interventions, minimizing the risk of adverse outcomes. Moreover, ATLS protocols promote standardization of care, ensuring consistent management practices across diverse clinical settings. Through multidisciplinary collaboration and continuous training, healthcare teams are equipped to deliver coordinated and efficient care, optimizing patient outcomes. In conclusion, using ATLS trauma management protocols represents more than a guideline; it embodies a commitment to excellence in patient care. By embracing the principles of ATLS, healthcare providers uphold the gold standard for safe, effective, and compassionate trauma management, ultimately saving lives and restoring hope in times of crisis.

Technological Progress and Engineering Applications: Safety Impacts for Workplaces and Society

Technological innovations, embodied in Industry 4.0 and transhumanist developments, have essentially changed the nature of engineering practices and industrial operations. This paper discusses the bifacial impact of such changes on the safety of occupations and ethical values in society, emphasizing critical shortcomings in traditional risk assessment methodologies. This study proposes a new quantitative framework for dynamic risk assessment of safety in interconnected industrial environments by adopting and adapting the Black-Scholes-Merton model, originally developed for financial markets. The parameters of the model—namely, safety state, volatility, and time to expiration—were recalibrated to match the industrial safety metrics and later validated through simulations and empirical case studies across various sectors, including manufacturing and construction. The results show that the BSM model is effective in predicting violations of safety thresholds, being much more adaptable and objective than traditional methods like Failure Mode and Effects Analysis. Sensitivity analyses indicate that both fluctuations and safety thresholds are of importance for determining risk probabilities, thus showing that careful monitoring and recalibration are necessary. Ethical considerations, including equity in transhumanist technologies and ecological impact of IoT systems, were embedded into the methodology, ensuring the results to be aligned with societal values and sustainability objectives. This research marries theoretical advances with practical applications, providing pragmatic insights for policy makers, engineers, and leaders in industry. It advocates proactive risk management, real-time integration of IoT, and interdisciplinary research in order to further improve the predictive models of safety. While some limitations were noted, including data dependency and assumptions related to normal distribution, the research has shown that financial-based methodologies can be transformational in the area of industrial safety. This dissertation advances the ongoing discussion surrounding Industry 4.0 by proposing novel instruments designed to manage its intricacies, all the while encouraging ethical and sustainable advancements in technology.

Digital Watermarking for a Bank Card Authentication System

Financial systems are under significant threats through bank card frauds using advanced cloning techniques such as skimming. In this paper, we propose a new method to improve the authentication of bank cards based on digital watermarks. The system puts an impermanent watermark into the magnetic stripe by exploiting its vulnerability, inserting a fragile watermark with the help of Least Significant Bit (LSB) algorithm. Character strings produced from authentic 13 personalized people records are included within the types of card data therefore, once each dealing, it's doable to verify the state of knowledge with watermark. Private key mechanism is using for encrypting and decrypt the watermark during transactions. Flu can also detect cloned cards with the help of its combination of MATLAB functions for embedding and extraction along with certain assessment methodologies which test the possibility to identify cloned card using bit error rate (BER) analysis. It will also work in reducing electronic payment security concerns with unauthorized card use.

Towards Sustainable Municipal Solid Waste Management: An SDG-Based Sustainability Assessment Methodology for Innovations in Sub-Saharan Africa

In sub-Saharan Africa, municipal solid waste management faces significant challenges, including inadequate infrastructure, increasing waste generation, and limited resources, leading to severe environmental and public health issues. Innovations in waste management are essential to address these pressing problems, as they can enhance efficiency, reduce pollution, and promote sustainable practices while fostering sustainable development. To select sustainable and contextually relevant solutions, it is vital to investigate their potential sustainability impacts based on the Sustainable Development Goals (SDGs) beforehand and to involve local stakeholders in the innovation process. Besides, engaging stakeholders increases community buy-in and fosters collaboration, leading to more effective and sustainable outcomes. This paper develops and applies a sustainability assessment methodology for innovations in municipal solid waste management systems in sub-Saharan Africa, with a case study in Ethiopia. The proposed methodology emphasizes the importance of involving local stakeholders in the SDG-based indicator assessment and offers suggestions for a data collection strategy. The case study on a composting process in Bishoftu Town demonstrates that stakeholder participation in selecting innovations positively influences the outcomes. However, the analysis indicates mixed effects of the innovation in the three sustainability dimensions, highlighting areas for optimization. Consequently, the presented method can support the innovation process of municipal solid waste management systems, fostering sustainable municipal development.

Environmental risk assessment methodology for urban tropical lagoons based on feasible lines of evidence under limited resources conditions: Jacarepaguá Lagoon/Brazil.

Determining environmental risk levels posed to different urban lagoon can provide an important overview regarding the relative severity of the environmental degradation of these ecosystems, increasing the risks visibility, which can be used as an important decision-making tool to prioritize investments. Jacarepaguá Lagoon (JPAL) is part of a coastal lagoon system comprising four interconnected lagoons in Rio de Janeiro city, Southeastern Brazil. Real estate speculation and insufficient sanitation infrastructure resulted in untreated sewage discharge into this ecologically sensitive lagoon system. An Environmental Risk Assessment for Jacarepaguá lagoon was carried out integrating three Lines of Evidence (LoE): (i) Water Quality LoE; (ii) Ecotoxicological LoE; (iii) Ecological LoE to estimate an Environmental Risk Index (EnvRI) for this area. Surface water samples were collected during four bimonthly campaigns at five JPAL sampling points as well as an upstream reference area. The Water Quality LoE based on physicochemical parameters was used to estimate the Water Quality Risk Index (WQRI); the Ecotoxicological LoE based on two chronic ecotoxicity bioassays was used to estimate the Ecotoxicological Risk Index (EcotoxRI); and the Ecological LoE based on the richness and the abundance of phytoplankton taxa was used to estimate the Ecological Risk Index (EcoRI). The final EnvRI was then estimated by integrating these three Risk Indices. The WQRI (0.79 ± 0.07), as well as the EcotoxRI (0.80 ± 0.21), and the EcoRI (0.78 ± 0.13), were all in the range classified as very high. The EnvRI for JPAL was consequently also very high (0.81 ± 0.12), which indicates urgent intervention. EnvRI combined with high concentrations of organic compounds directly affected the diversity of phytoplankton species; however, it was observed that the high content of phytoplankton biomass also represents a part of the organic matter in question. The final goal was achieved: a feasible risk assessment tool available for comparison of different aquatic ecosystems, to facilitate decision making establishing priorities of investments under a limited resources scenario.

A Systematic Review of Micropulse Laser Trabeculoplasty (MLT) in Primary Open-Angle Glaucoma (POAG) Management: Efficacy, Safety, and Future Perspectives.

Micropulse laser trabeculoplasty (MLT) is gaining attention as a non-invasive treatment option for primary open-angle glaucoma (POAG), offering an alternative to traditional surgeries and medications. This systematic review evaluates the effectiveness, safety, and potential of MLT in glaucoma management. This review adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The strength of evidence was assessed using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) methodology, following the 2011 Oxford Centre for Evidence-Based Medicine (OCEBM) recommendations. We identified 79 articles, and after removing duplicates and screening abstracts, 56 articles were eligible for further review. A detailed full-text analysis was conducted on 26 articles, of which 15 met the predefined inclusion criteria. MLT shows promise as a primary or adjunctive treatment for reducing intraocular pressure (IOP) in glaucoma and ocular hypertension patients. Current evidence supports its efficacy and safety; however, additional long-term studies are needed to confirm its durability and compare its effectiveness with traditional surgical and pharmacological approaches. Standardizing treatment protocols and refining patient selection criteria could enhance MLT's clinical value and support its broader adoption in glaucoma care.

Transition toward circular economy in the agrifood seedling phase: A Life Cycle Assessment on tomato trays.

National and international guidelines call for greater environmental sustainability in agriculture through the introduction of the circular economy. Recent studies investigate the introduction of new technologies and methodologies in the field, however, few focus on initial stages at the plant nursery or the management of the involved materials, and none scientifically calculate their environmental impact. To fill this gap, this paper focuses on an Italian case study that quantifies the environmental impact of the traditional seed trays used worldwide for growing, transporting and transplanting tomato seedlings, proposing and evaluating two alternatives from a circular economy perspective. To do this, the Life Cycle Assessment (LCA) methodology was applied to the traditional and to two new reusable, recyclable, washable seed trays, comparing their entire life cycles. Primary data, provided by three manufacturers, two nurseries and four agricultural farms, were modelled using SimaPro software. Their environmental impact was calculated considering the potentials of global warming, eutrophication, acidification, abiotic depletion of elements and fossil fuels, ozone layer depletion, water scarcity and photochemical oxidation. Results show that, irrespective of the type of material used for the seed pots, the extraction and production phase of the materials is the most impactful in the life cycles. The proposed trays resulted more environmentally sustainable than the traditional non-reusable expanded polystyrene tray. The work also quantified the environmental benefit achieved with the weight reduction of the seed pots and their better handling by operators, which avoided breakages and losses. Furthermore, it is shown how the proposed reusable solutions can be greener, reducing the impacts up to a third in the case of global warming potential. In conclusion, the research offers viable alternatives that can be used in the market all over the world, diminishing the overall impact of the seedling and transplanting phases.