Methodological Toolbox Research Articles

Material Philology and Jubilees Manuscripts from Qumran: Exploring Questions of Theory and Method

Material philology as an approach to the study of ancient manuscripts and their texts has grown in popularity over the past decade and its application to the study of the manuscripts of Jubilees opens for a number of important perspectives that nuance our picture of the literary development of the work. This article discusses material philology as both a theoretical position and a methodological toolbox that can both guide the study of the manuscripts and texts of Jubilees and allow for different avenues of analysis. Based on these theoretical and methodological insights, three examples are given of ways in which a material philological approach influences our understanding of Jubilees during the Second Temple Period at the level of the manuscript, text, and work.

Thinking With Transnational Institutional Ethnography: Moving Towards Spatially Conscious Methods for Studying Geographically Dispersed People and Institutions

Institutional ethnography (IE) is a valuable ontological approach for investigating the coordination of social relations, both locally and trans-locally. However, much of the work utilizing IE remains within the confines of a local or national empirical field. This means there are limited accounts of IE exploring geographically dispersed institutions that cross borders and span regions. This paper makes the case for critically thinking and building on Transnational Institutional Ethnography (TIE). In it, I argue that TIE requires adapting the methodological toolbox of IE to deal with expanded geographic space and diverse bodily locations. By drawing on empirical work exploring scholarly participation in the European Union Framework Programs for research, tensions are illustrated that surfaced while working with TIE. In this discussion, I examine the ways in which interpreting an empirical field as a transnational institution, constituted of geographically dispersed people and organizations, caused a re-examination of the methods in IE to acknowledge institutional experiences shaped by diverse material surroundings and differing geopolitical locations. The paper argues that working with spatially conscious methods in TIE contributes positively to both fieldwork and analysis.

Organizing the Methodological Toolbox: Lessons Learned From Implementing Developmental Methods Online.

Adapting studies typically run in the lab, preschool, or museum to online data collection presents a variety of challenges. The solutions to those challenges depend heavily on the specific questions pursued, the methods used, and the constraints imposed by available technology. We present a partial sample of solutions, discussing approaches we have developed for adapting studies targeting a range of different developmental populations, from infants to school-aged children, and utilizing various online methods such as high-framerate video presentation, having participants interact with a display on their own computer, having the experimenter interact with both the participant and an actor, recording free-play with physical objects, recording infant looking times both offline and live, and more. We also raise issues and solutions regarding recruitment and representativeness in online samples. By identifying the concrete needs of a given approach, tools that meet each of those individual needs, and interfaces between those tools, we have been able to implement many (but not all) of our studies using online data collection during the COVID-19 pandemic. This systematic review aligning available tools and approaches with different methods can inform the design of future studies, in and outside of the lab.

8Postcolonial Theory

Abstract This chapter covers selected research in postcolonial theory published in 2020 and some work delayed and published in early 2021. It begins with a discussion of a new edition of an introduction to postcolonialism and a journal issue with an article cluster devoted to the legacy of Edward Said’s Orientalism (1978) before it focuses on two books that rethink postcolonial studies. The work discussed demonstrates how the ideas in the postcolonial theory and methodology toolbox can still be put to good scholarly use in explorations of bottom-up postcolonialism and activism as well as in top-down postcolonialism and the reading of texts. The book-length studies propose reframing exercises and practical demonstrations that point to new avenues where postcolonial perspectives are still invigorating and, possibly, invaluable.

Adaptation to climate change in coastal towns of between 10,000 and 50,000 inhabitants

This chapter focuses on mainland coastal towns that have populations of between 10,000 and 50,000 inhabitants. Through six case studies, the chapter develops an understanding of the characteristics that shape each of the communities. By exploring the climate change hazards each of the case studies are experiencing now and forecast into the future, the physical and ecological profile and the human development condition, a view of the climate impacts for each is shaped. The six case study areas include towns that are large enough to be a regional centre, down to much less organised and resourced communities that are at a more subsistence level of development.The case studies include communities that range from tropical storm areas to more temperate climatic areas. Adaptation strategies for each case study are observed, drawing out the similarities and the differences. Insights and lessons learnt show that the bottom-up inclusion of all communities in shaping the adaptation approach is crucial, together with alignment of policy between governments from national to local. Also crucial is the resourcing of local councils which sit on the front line of many adaptation initiatives. The role of universities and other research organisations to provide data, skills training and a toolbox of methodologies to those in the front line leadership roles. An important insight is the collaborative opportunity for universities, communities and agencies to draw out the innovative adaptation strategies that can inform other coastal communities from the smallest village to the largest city.

Optical Clearing and Light Sheet Microscopy Imaging of Amphioxus.

Cephalochordates (amphioxi or lancelets) are representatives of the most basally divergent group of the chordate phylum. Studies of amphioxus development and anatomy hence provide a key insight into vertebrate evolution. More widespread use of amphioxus in the evo–devo field would be greatly facilitated by expanding the methodological toolbox available in this model system. For example, evo–devo research on amphioxus requires deep understanding of animal anatomy. Although conventional confocal microscopy can visualize transparent amphioxus embryos and early larvae, the imaging of later developmental stages is problematic because of the size and opaqueness of the animal. Here, we show that light sheet microscopy combined with tissue clearing methods enables exploration of large amphioxus specimens while keeping the surface and the internal structures intact. We took advantage of the phenomenon of autofluorescence of amphioxus larva to highlight anatomical details. In order to investigate molecular markers at the single-cell level, we performed antibody-based immunodetection of melanopsin and acetylated-α-tubulin to label rhabdomeric photoreceptors and the neuronal scaffold. Our approach that combines light sheet microscopy with the clearing protocol, autofluorescence properties of amphioxus, and antibody immunodetection allows visualizing anatomical structures and even individual cells in the 3D space of the entire animal body.

Mapping the online songbird trade in Indonesia

Wildlife trade, when unsustainable, can be an important threat to biodiversity conservation. In this contribution, we explored the use of digital data to investigate the online market for songbirds in Indonesia, where keeping pet songbirds is a deeply rooted cultural practice. We examined the spatial characteristics of three dimensions of the songbird trade using data from online sources: birdwatchers’ sightings as a proxy for the supply of the songbird market, small advertisements from an online marketplace platform, representing the trade itself and its transactions, and videos by pet songbird owners to represent the demand side of the songbird market. We found that, geographically, these three stages of the songbird supply chain did not overlap, which potentially hints at the roles extended transport networks and commercial captive breeding play for the songbird trade. The trade was not confined to major cities but spread out through the country, indicating both a possible democratisation of the trade (i.e. a larger group of sellers, and consumers selling to consumers) and an opportunity to observe previously covert parts of the trade. We further found that the asking prices on online marketplaces were significantly higher than the prices stated in an independently carried out consumer survey and discuss possible reasons for these differences. Data from digital sources can give rich insights into the spatial, temporal and taxonomic structure of wildlife trade, can help understand the motivations of buyers and sellers, and can help direct wildlife trade towards a more sustainable fashion. Our methodology toolbox that allows automatic and continuous monitoring of online marketplaces, includes data preparation and cleaning, and follows the highest standards of data privacy principles, is openly available.

Different pathways to identify moral framing from media content: A response to Hopp and Weber

ABSTRACT Moral framing is a mobilizing strategy in digital activism to raise awareness of a social issue. This essay discussed the strengths and weaknesses of multiple methods of extracting moral framing from media content. Two issues were highlighted from crowd-coding and automated coding using computational approaches: context sensitivity and the handling of coding discrepancies. We compared results from manual content coding and automated analysis using the eMFD, urging scholars to incorporate multiple methods to uncover the meanings behind each moral value. We argued that crowdcoding, computational approaches and manual coding are not exclusive of one another, and encouraged scholars to diversify their methodological toolbox and choose what to use based on the research context.

Light-driven decarboxylative deuteration enabled by a divergently engineered photodecarboxylase

Despite the well-established chemical processes for C-D bond formation, the toolbox of enzymatic methodologies for deuterium incorporation has remained underdeveloped. Here we describe a photodecarboxylase from Chlorella variabilis NC64A (CvFAP)-catalyzed approach for the decarboxylative deuteration of various carboxylic acids by employing D2O as a cheap and readily available deuterium source. Divergent protein engineering of WT-CvFAP is implemented using Focused Rational Iterative Site-specific Mutagenesis (FRISM) as a strategy for expanding the substrate scope. Using specific mutants, several series of substrates including different chain length acids, racemic substrates as well as bulky cyclic acids are successfully converted into the deuterated products (>40 examples). In many cases WT-CvFAP fails completely. This approach also enables the enantiocomplementary kinetic resolution of racemic acids to afford chiral deuterated products, which can hardly be accomplished by existing methods. MD simulations explain the results of improved catalytic activity and stereoselectivity of WT CvFAP and mutants.

Matching Methods for Confounder Adjustment: An Addition to the Epidemiologist's Toolbox.

Propensity score weighting and outcome regression are popular ways to adjust for observed confounders in epidemiologic research. Here, we provide an introduction to matching methods, which serve the same purpose but can offer advantages in robustness and performance. A key difference between matching and weighting methods is that matching methods do not directly rely on the propensity score and so are less sensitive to its misspecification or to the presence of extreme values. Matching methods offer many options for customization, which allow a researcher to incorporate substantive knowledge and carefully manage bias/variance trade-offs in estimating the effects of nonrandomized exposures. We review these options and their implications, provide guidance for their use, and compare matching methods with weighting methods. Because of their potential advantages over other methods, matching methods should have their place in an epidemiologist's methodological toolbox.

Inter the Mesolithic —Unearth Social Histories: Vexing Androcentric Sexing through Strøby Egede

This article dissects the methodological toolbox of essences referred to as the Mesolithic, attempting to grasp its calculus. The autopsy then sets about investigating the reductionist problems of essentialism and androcentrism that have spread throughout the Mesolithic project like a malign cancer. Especially the insistent practice to sex supposedly important things and activities as male is confronted. The mass grave at Strøby Egede, eastern Zealand, Denmark, serves as a very detailed example. An effort is thereafter made to convey some of its sociohistorical specificity.

Country of Words: Palestinian Literature in the Digital Age of the Refugee

Abstract The article reflects on how to embrace the unconventional, fragmented, scattered, transnational, exilic, and refugee elements of Palestinian Literature. Placing the refugees at the heart of the story of Palestinian literature raises serious questions about the compatibility of the national framework as the primary mode of analysis. The article explores the anatomy of Palestinian literature, including the wide array of sources, literary detective work, and expanded methodological toolbox needed to gather its fragments, and illustrates the potential of the digital sphere—drawing on the world of Digital Humanities—to house, express and visualize the data-fragments of Palestinian literature.

Using 3D Rule-Based Modeling to Interactively Visualize “Complete Streets” Design Scenarios

“Complete Streets” has successfully emerged, and is increasingly being adopted around the U.S.A., as a transportation policy and design approach that aims to support the needs of all road users. Requiring complex street design configurations, Complete Streets initiatives can benefit from the power of three-dimensional (3D) visualization to share the design vision with stakeholders and citizens. Traditional modeling techniques present some challenges to respond to such needs because of low modeling efficiency. However, 3D procedural modeling, also known as rule-based modeling, provides exciting opportunities to overcome such challenges. This study investigates 3D rule-based modeling as a scenario-oriented street design tool. It employs a case study approach that utilizes a set of rules designed for Complete Streets and applies rule-based modeling to visually compare design scenarios using a study area in Florida. Findings show that the rule-based modeling approach is proven to effectively visualize scenario-oriented street designs. Its ability to modify design parameters easily and generate scenarios rapidly, enables effective visual comparison of alternatives. Its ability to be customized and extended makes it applicable to thousands of communities around the country that are looking to implement Complete Streets designs. Finally, with the ability to support 3D web-based visualization and virtual reality, the rule-based approach can serve as an effective integrated collaboration platform. The Complete Streets rules are available and can be utilized by practitioners immediately. For researchers, the rule-based street modeling approach adds another tool in their methodological toolbox that can help bridge modeling and visualization with Complete Streets research.

Electric carsharing and the sustainable mobility transition: Conflict and contestation in a Czech actor-network

The energy transition has seen greater progress in some areas than others. Although it accounts for a significant portion of global greenhouse gas emissions, the transportation sector has hardly broken its high dependence on oil. Within the transportation sector, passenger road transport plays a considerable role in terms of both energy consumption and emissions. This paper, therefore, aims to contribute to the knowledge and theory of sustainability transitions in passenger road transportation by addressing the question, “How do actors and technologies interact to shape the process of sustainability transitions in road transport?” To help answer this question, Actor-Network Theory is applied to a local-level case study of an electric carsharing company in Brno, Czech Republic. A review of the energy transitions literature revealed ANT as a fruitful methodological toolbox due to its unique ontology and concepts. The study reveals that both the human and non-human actors within this network indeed play crucial roles that influence its successful expansion. These roles are often contested and negotiated by the various actors, thereby dictating the development of these associations as well as the actor-network as a whole. Moreover, the roles and associations within this network in many instances emerge not through human intention or agency but in response to those negotiated issues particularly surrounding the material elements of the network. While the study focuses on a specific case, it also offers insights and lessons for the broader energy transition, particularly the process of such transition in passenger road transportation.

Embracing multidimensionality in phonological analysis

AbstractWe pursue the idea, implicit in much current phonological research, that understanding the multiple factors that shape speech production and perception is within the purview of phonology. In particular, increased access to naturalistic data has highlighted the multidimensional reality of variation in spoken language. At the same time, longstanding methods of doing phonology – including impressionistic analysis, and laboratory and experimental studies – remain crucial to understanding native speaker competence and grammar. We advocate for an expanded methodological toolbox in phonological analysis, using an iterative approach that crucially includes naturalistic corpus data. Integrating across multiple data sources offers fuller insight into the nature of the phonological system and native speaker-hearer ability. Several case studies highlight findings gained through linked, iterative studies, showing the importance of naturalistic data for a richer understanding of phonological phenomena, and leading us to reflect on desiderata for corpora to reveal speaker-specific patterns in fine phonetic detail and variability, which we argue are part of a speaker-hearer’s phonological competence. Phonological analysis that embraces the full spectrum of variation in spoken language data (from categorical to gradient, and systematic to sporadic) contributes to a deeper understanding of phonology in this richer sense.

A practical approach to measuring the acoustic diversity by community ecology methods

Abstract The study of the diversity of animal signals on within‐ and among‐species levels is the key to uncover mechanisms that shape the evolution of communication systems. However, the methods used to quantify acoustic diversity (like repertoire size) lack to grasp several aspects of acoustic diversity. Here, we propose a new framework for the study of animal communication, in which we decompose the acoustic diversity with the methodological toolbox from community ecology. We explore how different diversity estimates reflecting different aspects of acoustic diversity can be applied to characterise the complexity of acoustic signals. We propose that this approach can be used in a wide range of animal taxa to derive further insights about the function and evolution of communication systems besides the traditional methods. To illustrate the use of our approach in a case study, we used the song of the collared flycatcher Ficedula albicollis as model system. Based on three frequency and time variables, we calculated three functional diversity indices (FRic, FEve and FDiv) to characterise the distribution of song elements (syllables) in the acoustic parameter space. We aimed to uncover the interrelations of diversity indices, reveal the degree of among‐individual consistency and investigate their relationships with certain aspects of individual quality (body condition, age and arrival date). We found that the diversity indices were largely independent of each other and showed different consistency patterns that were time‐scale dependent, indicating different signalling potential of individual‐specific attributes. We also found that FEve strongly related to the age of males. Our case study showed that decomposing the diversity into different components can reveal additional biologically meaningful aspects of birdsong.

A Facile Strategy to Achieve Synergistic Multiple Hydrogen Bonding Interactions for Constructing Robust Hydrogels with Self‐healing Capability, Shape Transformation and Actuation Function

AbstractThe double network (DN) hydrogels, especially the physically crosslinked DN hydrogels, have attracted increasing attention due to their high reconfigurability, multi‐sensitivity, and efficient energy dissipation, but their applications still suffer from the relatively low mechanical strength, complex preparation process, and insufficient functionality. In this work, a simple but efficient strategy to fabricate robust and multitasking polyvinyl alcohol (PVA)/poly(N‐acryloyl glycinamide) (PNAGA) DN hydrogels via an ultraviolet (UV)‐initiated one‐pot in situ polymerization and a subsequent freezing‐thawing treatment is reported. The PVA/PNAGA DN hydrogels are fully physically linked DN network via multiple hydrogen bonding interactions, which contain hard PVA crystalline microdomains and soft PNAGA regions. The synergetic multiple hydrogen bonding interactions and the cooperation of hard and soft regions in PVA/PNAGA hydrogels as well as the polyhydroxy matrix can not only impart the PVA/PNAGA hydrogels with super mechanical strength, high toughness, good stretchability, and rapid shape‐recoverability, but also endow the hydrogels with tailorable self‐healability, multiple shape memory, and actuation function. Considering the easily available materials, simple preparation process, and highly multitasking properties, this novel strategy should greatly enrich the methodological toolbox for the synthesis of robust and multifunctional hydrogels for the diversity of applications.

A Latent Space Modeling Approach to Interfirm Relationship Analysis

Interfirm relationships are crucial to our understanding of firms’ collective and interactive behavior. Many information systems-related phenomena, including the diffusion of innovations, standard alliances, technology collaboration, and outsourcing, involve a multitude of relationships between firms. This study proposes a latent space approach to model temporal change in a dual-view interfirm network. We assume that interfirm relationships depend on an underlying latent space; firms that are close to each other in the latent space are more likely to develop a relationship. We construct the latent space by embedding two dynamic networks of firms in an integrated manner, resulting in a more comprehensive view of an interfirm relationship. We validate our approach by introducing three business measures derived from the latent space model to study alliance formation and stock comovement. We illustrate how the trajectories of firms provide insights into alliance activities. We also show that our proposed measures have strong predictive power on stock comovement. We believe the proposed approach enriches the methodology toolbox of IS researchers in studying interfirm relationships.

Toward a Multilingual Victorian Transatlanticism

This essay argues that scholarship being done under the sign of transatlantic studies, and Victorian transatlantic studies in particular, is problematically focused on the anglophone northern Atlantic region. Challenging both the essentialness and the disciplinary primacy of the “special relationship” between Britain and the United States, I argue instead that the entire nineteenth-century Atlantic world was a geographically and linguistically permeable space. Paying attention to crossings from north to south and vice versa is both methodologically and ethically necessary. From a methodological perspective, it can help us produce much more thorough answers to the questions transatlantic studies purports to ask about identity and community. But reading beyond anglophone British and U.S. American texts can also help us decolonize our reading and thinking. Of course, work like this requires scholars to read in second and third languages; as such, this essay discusses and denaturalizes the institutional barriers to multilingual English studies. It also offers a case study—a brief reading of a novel by Argentine writer Vicente Fidel López—demonstrating the insights that can be gained by expanding both our geographic perspective and our methodological toolbox.

How to Problematise Categories: Building the Methodological Toolbox for Linguistic Reflexivity

Following qualitative researchers’ growing interest in reflexivity, a body of scholarship has emerged that aims to turn informal practices for reflexivity into methods that can be learnt and taught alongside other research practices. This literature, however, has focused on helping researchers become more reflexive toward their situatedness and positionality, rather than toward their use of language and its effects on knowledge production – a process I refer to as ‘linguistic reflexivity’. This article addresses this gap by formalising a method for ‘problematising categories’, an informal approach familiar to qualitative researchers as a promising solution to the analytical and ethical blinders that result from scholars’ unconscious use of language. I proceed in three steps. First, I review the literature to show the analytical, empirical and ethical rationales behind this approach and offer a definition of problematising categories as the practice of making conscious how socio-linguistic units of categorisation unconsciously organise our perception and can represent a problem for knowledge production. This practice, I argue, enables us to decentre ourselves from the taken-for-granted nature of those categories. Second, I develop a three-stage research method for problematising categories: noticing ‘critical junctures' when problematisation is called for, identifying the categorical problem through sensitising questions and reconstructing an alternative. Third, I demonstrate how problematising categories contributes to the research process by applying this method to my experience in problematising the binary pair ‘local’ versus ‘international’ in a research project on the environmental impact of Chinese investment in the Senegalese fishery sector. I show that problematising categories leads to more rigorous empirical findings and nuanced analysis in a way that is feasible within the frame of qualitative research projects. Overall, this article expands the practical tools for linguistic reflexivity and heeds the methodological call to make conscious and explicit choices for every dimension of our research.