Methodological Considerations Research Articles

On The Ground: Methodological and Ethical Considerations of a Field Study on Alcohol Intoxication and Sexual Misperception among Drinking Venue Patrons.

The goal of this paper is to provide guidance to researchers on the logistical and ethical considerations involved in conducting an alcohol field study by detailing the methodology employed in a field study on alcohol and sexual misperception. This article presents a field study protocol for measuring sexual misperception, a precursor to SA, in patrons exiting bars. One hundred forty-three participants completed study procedures. In-depth information on procedures for site selection, recruitment, screening, measure selection, consent, and capacity assessment is provided. Field studies can evaluate proximal causes of alcohol-involved sexual aggression and intermediary processes like sexual misperception in naturalistic settings. Greater implementation of field studies is required for a comprehensive understanding of alcohol-involved SA perpetration and for generalizable interventions.

Neuromodulation in Small Animal fMRI.

The integration of functional magnetic resonance imaging (fMRI) with advanced neuroscience technologies in experimental small animal models offers a unique path to interrogate the causal relationships between regional brain activity and brain-wide network measures-a goal challenging to accomplish in human subjects. This review traces the historical development of the neuromodulation techniques commonly used in rodents, such as electrical deep brain stimulation, optogenetics, and chemogenetics, and focuses on their application with fMRI. We discuss their advantageousness roles in uncovering the signaling architecture within the brain and the methodological considerations necessary when conducting these experiments. By presenting several rodent-based case studies, we aim to demonstrate the potential of the multimodal neuromodulation approach in shedding light on neurovascular coupling, the neural basis of brain network functions, and their connections to behaviors. Key findings highlight the cell-type and circuit-specific modulation of brain-wide activity patterns and their behavioral correlates. We also discuss several future directions and feature the use of mediation and moderation analytical models beyond the intuitive evoked response mapping, to better leverage the rich information available in fMRI data with neuromodulation. Using fMRI alongside neuromodulation techniques provide insights into the mesoscopic (relating to the intermediate scale between single neurons and large-scale brain networks) and macroscopic fMRI measures that correlate with specific neuronal events. This integration bridges the gap between different scales of neuroscience research, facilitating the exploration and testing of novel therapeutic strategies aimed at altering network-mediated behaviors. In conclusion, the combination of fMRI with neuromodulation techniques provides crucial insights into mesoscopic and macroscopic brain dynamics, advancing our understanding of brain function in health and disease. EVIDENCE LEVEL: 1 TECHNICAL EFFICACY: Stage 1.

“The seven principles of universal design of research”: how to increase user diversity in mainstream research

PurposeUser diversity has been discussed in many fields of study for decades. Nevertheless, some cohorts are rarely included in mainstream research. According to the concept of universal design of research (UDR), people with disabilities should be more often included in participant pools. There is, however, a need for certain methodological considerations to achieve UDR.Design/methodology/approachThe suggested guidelines are based upon existing guidelines and research in addition to key takeaways from studies conducted by the authors over the past 15 years. Six studies are used to show how the guidelines can be applied in research involving three example cohorts, namely people with dyslexia, aphasia and intellectual disability.FindingsExtensive guidelines are needed to increase user inclusion in mainstream studies and support researchers in conducting purposeful and ethical research. The suggested guidelines should be examined for other cohorts to ensure that all types of user needs are accommodated.Research limitations/implicationsThis study contributes with methodological approaches to enhance inclusion of all types of participants in mainstream research.Originality/valueThe main contribution is The seven principles of UDR, a further development of the original principles for UDR.

Exploring Circadian Changes in Muscle Physiology: Methodological Considerations.

Exploring Circadian Changes in Muscle Physiology: Methodological Considerations.

Current developments in prehabilitation in urologic oncology.

Prehabilitation describes interventions that are undertaken prior to a major surgical or medical intervention with the objective of improving functional capability with the goal of improving candidacy for therapy, bolstering one's ability to withstand treatment-associated toxicity, functional decline, and facilitating accelerated recovery. The objective of this review is to detail the key tenets of prehabilitation, synthesize contemporary advances in prehabilitation science within Urologic Oncology, and discuss key methodologic trial design considerations salient to future prehabilitation investigations. Contemporary prehabilitation clinical trials have primarily evaluated unimodal interventions aiming to improve functional capacity across the domains of physical exercise, nutrition, and cognition with heightened interest in evaluating multimodal interventions addressing two or more domains. Recent investigations have have demonstrated variable improvements in strength, balance, physical function, and quality of life with preoperative exercise. Although presurgical immunonutrition showed promise in other fields, initial results in uro-oncology have not demonstrated reductions in complications nor improvements in early survival. Emerging data supports the potential of multimodal prehabilitation programs to offer more comprehensive benefits, improving functional outcomes, reducing length of stay, and supporting improved recovery. To date, early prehabilitation studies in patients undergoing surgery for genitourinary malignancies have demonstrated variable ability to facilitate gains in functional capacity and perioperative outcomes. Key issues have arisen including the need to ensure that interventions are pragmatic, scalable, feasible, and acceptable in these populations that often also have a high prevalence of coincident multimorbidity, frailty, and mental health concerns that can increase risk of adverse outcomes after surgery. The integration of personalized prehabilitation strategies as extensions of perioperative enhanced recovery after surgery protocols, supportive care and survivorship paradigms offers of promise to further engage patients in their care, enhance patient resilience and outcomes, while reducing treatment burden in urologic oncology.

The moving wave: Applications of the mobile EEG approach to study human attention.

Although historically confined to traditional research laboratories, electroencephalography (EEG) paradigms are now being applied to study a wide array of behaviors, from daily activities to specialized tasks in diverse fields such as sports science, neurorehabilitation, and education. This transition from traditional to real-world mobile research can provide new tools for understanding attentional processes as they occur naturally. Early mobile EEG research has made progress, despite the large size and wired connections. Recent developments in hardware and software have expanded the possibilities of mobile EEG, enabling a broader range of applications. Despite these advancements, limitations influencing mobile EEG remain that must be overcome to achieve adequate reliability and validity. In this review, we first assess the feasibility of mobile paradigms, including electrode selection, artifact correction techniques, and methodological considerations. This review underscores the importance of ecological, construct, and predictive validity in ensuring the trustworthiness and applicability of mobile EEG findings. Second, we explore studies on attention in naturalistic settings, focusing on replicating classic P3 component studies in mobile paradigms like stationary biking in our lab, and activities such as walking, cycling, and dual-tasking outside of the lab. We emphasize how the mobile approach complements traditional laboratory paradigms and the types of insights gained in naturalistic research settings. Third, we discuss promising applications of portable EEG in workplace safety and other areas including road safety, rehabilitation medicine, and brain-computer interfaces. In summary, this review explores the expanding possibilities of mobile EEG while recognizing the existing challenges in fully realizing its potential.

TRAINING OF FUTURE SPECIALISTS FOR THE USE OF STEM LABORATORIES IN PRIMARY EDUCATION

The article discusses the preparation of future specialists for the use of a STEM laboratory at the initial stage and is based on determining the effectiveness in accordance with the results of research. Scientific papers, state regulatory documents and programs that reveal the content of the topic on the issue of training future specialists to use STEM laboratories in primary education are analyzed. The possibilities of the process of training future specialists to use the STEM laboratory in primary education are determined.In the preparation of future primary school specialists, the problem of organizing students’ research activities at a high level through the development of their creative and intellectual potential is considered. The effective use of the STEM laboratory in primary school, the effectiveness of the implementation of research activities of students in teaching using STEM technology has been established.In order to reveal the research topic, based on the analyzed scientific papers, special surveys were obtained with the participation of primary school teachers in order to determine the possibility of using STEM laboratory by future specialists in primary education. In addition, as a result of the theoretical differentiation of scientific papers, the content and effectiveness of the use of the STEM laboratory in education are methodically determined in the article, and the possibilities of its’ application are analyzed. The features of STEM education in primary grades are determined. The scientific novelty lies inthe interrelated theoretical and methodological consideration of the possibilities of preparing future specialists for the use of STEM laboratories in primary education.

From Food Deserts to Food Access. Methodological and Theoretical Considerations for Research in Sparsely Populated Rural European Areas

Abstract The disappearance of food shops in small towns and villages is a common feature in many European areas. The food habits of rural inhabitants are affected by the limitations on their food supply, posing a challenge to how they organise their daily eating. Although this represents a major problem for the quality of life in rural areas, food access problems have been understudied in Europe. This paper presents some research notes to encourage the study of the effects of the shortfall in food businesses in depopulated rural areas of Europe as a guide for research on this phenomenon. The paper reviews the international literature, drawing attention to the theoretical problems and methodological limitations involved in this type of study. A series of recommendations are given with the aim of enabling future research to record the food situation of the inhabitants of impoverished and depopulated territories with greater precision and depth.

A Systematic Literature Review of Empirical Studies on Learning Engagement in Educational Settings

Learning engagement is a crucial aspect of educational success, yet its complexities remain a subject of ongoing investigation. This systematic literature review presents a comprehensive synthesis of empirical research on learning engagement, meticulously curated through a thorough review process. Drawing upon a diverse array of scholarly works, this review delves into the antecedents and outcomes of learning engagement, shedding light on its multifaceted nature. Various measurement methods, including surveys, interviews, and observation, are explored, emphasizing the diverse approaches to understanding and assessing engagement. Key findings underscore the pivotal roles of intrinsic motivation, perceived value, self-regulation, and social support in nurturing learning engagement among learners. Moreover, methodological considerations are critically examined, paving the way for future research avenues aimed at deepening our comprehension of learning engagement dynamics within diverse educational contexts. This review serves as a valuable resource for educators, researchers, and policymakers seeking to enhance student engagement and academic outcomes.

Lessons learned during a 12-year monitoring project with the endangered Magdalena River turtle (Podocnemis lewyana): hunting pressure, habitat degradation, and methodological considerations

Turtle species in the Family Podocnemididae, including the Colombian endemic and critically endangered Magdalena River Turtle Podocnemis lewyana, characteristically present low recapture rates that preclude estimation of population parameters using maximum likelihood modeling. In our 12-year monitoring project with this species, we evaluated changes in relative abundances, proportions of sex/size classes, and individual body sizes and body conditions in a population in four channels in the middle Magdalena River drainage. We also inspected for associations between trends in changes in these variables and differences in hunting pressure and habitat degradation. To inspect for temporal and spatial demographic dynamics, we estimated variation in relative abundances using the Catch Per Unit Effort index, the total number of turtles captured over an entire 5-day sampling period using ten baited funnel traps. Relative abundances and the proportions of sex/size classes were different between sites and years. We found a significant decline in the proportion of females and juveniles over time, along with evidence that the females still present were smaller in body size. Our results support the hypothesis that hunting eliminates adult females from these sites, perhaps also translating into a reduction in recruitment. The lack of evidence of generalized declines in body condition of all size classes suggests that habitat degradation might contribute less to the population declines in this region. Our results also illustrate that even when recapture rates are low, monitoring turtles via standardized trapping may yield insights into the population’s conservation status that other relative abundance indices cannot.

Gerotherapeutics: Aging Mechanism-based Pharmaceutical and Behavioral Interventions to Reduce Cancer Racial and Ethnic Disparities.

The central premise of this article is that a portion of the established relationships between social determinants of health and racial/ethnic disparities in cancer morbidity and mortality are mediated through differences in rates of biological aging processes. We further posit that using knowledge about aging could enable discovery and testing of new mechanism-based pharmaceutical and behavioral interventions ("gerotherapeutics") to differentially improve the health of minoritized cancer survivors and reduce cancer disparities. These hypotheses are based on evidence that lifelong differences in adverse social determinants of health contribute to disparities in rates of biological aging ("social determinants of aging"), with minoritized groups having accelerated aging (ie, a steeper slope or trajectory of biological aging over time relative to chronological age) more often than non-minoritized groups. Acceleration of biological aging can increase the risk, age of onset, aggressivity and/or stage of many adult cancers. There are also documented negative feedback loops whereby the cellular damage caused by cancer and its therapies act as drivers of additional biological aging. Together, these dynamic intersectional forces can contribute to differences in cancer outcomes between minoritized vs non-minoritized survivor populations. We highlight key targetable biological aging mechanisms with potential applications to reducing cancer disparities and discuss methodological considerations for pre-clinical and clinical testing of the impact of gerotherapeutics on cancer outcomes in minoritized populations. Ultimately, the promise of reducing cancer disparities will require broad societal policy changes that address the structural causes of accelerated biological aging and ensure equitable access to all new cancer control paradigms.

Effect of Semaglutide in Individuals With Obesity or Overweight Without Diabetes.

This systematic review evaluates the efficacy and safety of semaglutide in individuals with obesity or overweight without diabetes. Obesity is a significant public health concern, associated with various comorbidities and reduced quality of life. Semaglutide, a glucagon-like peptide-1 receptor agonist, has emerged as a promising pharmacological intervention for weight management. This review synthesizes findings from multiple clinical trials, highlighting the impact of semaglutide on weight loss, metabolic parameters, and overall health outcomes in non-diabetic populations. The review also addresses methodological considerations, including study design, participant selection, and outcome measures, to assess the robustness of the evidence. Ethical considerations and potential conflicts of interest are discussed to ensure transparency in the research process. The findings indicate that semaglutide is associated with significant weight reduction and improvement in obesity-related health markers, suggesting its potential as a valuable treatment option for individuals struggling with obesity. Limitations of the current literature and recommendations for future research directions are also presented, emphasizing the need for further studies to explore the long-term effects and generalizability of semaglutide treatment in diverse populations.

Methodological Considerations for Difference-in-Differences

Methodological Considerations for Difference-in-Differences

Methodologic considerations in estimating racial disparity of mortality among very preterm infants.

This review explores methodological considerations in estimating racial disparities in mortality among very preterm infants (VPIs). Significant methodological variations are evident across studies, potentially affecting the estimated mortality rates of VPIs across racial groups and influencing the perceived direction and magnitude of racial disparities. Key methodological approaches include the birth-based approach versus the fetuses-at-risk approach, with each offering distinct insights depending on the specific research questions posed. Cohort selection and the decision for crude versus adjusted comparison are also critical elements that shape the outcomes and interpretations of these studies. This review underscores the importance of careful methodological planning and highlights that no single approach is definitively superior; rather, each has its strengths and limitations depending on the research objectives. The findings suggest that adjusting the methodological approach to align with specific research questions and contexts is essential for accurately assessing and addressing racial disparities in neonatal mortality. IMPACT: Elucidates the impact of methodological choices on perceived racial disparities in neonatal mortality. Offers a comprehensive comparison of birth-based vs. fetuses-at-risk approaches in the context of racial disparity research. Provides guidance on the cohort selection and adjustment criteria critical for interpreting studies on racial disparities in very preterm infant mortality.

Comparative VOD catalogue research: Circulation, presence and prominence of British content in Europe

Video-on-demand (VoD) platforms have become primary spaces for encounters with transnational film and television, particularly among younger audiences. The expansion of global US-owned VoD services like Netflix has generated questions about the availability, discoverability, and prominence of domestic and European content, making the issue of how to analyse VoD catalogues pressing. Two perspectives are prevalent in VoD catalogue research: ‘back-end’ research emphasising composition and circulation; and ‘front-end’ analysing content presentation and discoverability. Quantitative methods facilitate comparative and longitudinal analyses of what is found on which VoD catalogues. Qualitative methods examine where and how audiences find content. This article, fostering synergy between the two, probes (1) the significance of VoD catalogue research in understanding the dynamics of transnational content flows and audience behaviours, and (2) its methodological possibilities and limitations. Focusing on British content in European VoD catalogues, it draws on two research projects: EUVoD (Aarhus Universitets Forskningsfond AUFF, 2021–2024), analysing the developing European VoD market within changing conditions of competition and policy; and Screen Encounters with Britain (AHRC, 2022–2025), investigating young Europeans’ use of British film and TV. The article utilises the European Audiovisual Observatory’s databases of European works and the streaming guide JustWatch.com, combined with systematic tracking of VoD landing pages. It thus maps content availability, popularity, and promotional strategies across different European markets and services, showcasing how patterns of presence, prominence and circulation shape probable audience encounters with non-domestic content. The article probes methodological considerations, complexities and caveats applicable to wider catalogue research into transnational media. In the case of British content availability on VoD, it shows how a vast number of ca. 15,000 individual UK titles available in European catalogues boils down to about 200 titles that can be easily found on Netflix, and 70 titles there of that young audiences (aged 16–34) recall as UK shows they know and remember.

Methodological considerations in cross-sectional studies validating the International Classification of Functioning, Disability and Health (ICF) Core-Sets: a systematic review.

To describe and analyze the methodological characteristics and quality of cross-sectional studies that have validated an ICF-CS. A systematic review was conducted to identify empirical studies published in English that validated any ICF-CS using a cross-sectional design. Databases searched included Web of Science, Scopus, CINAHL, PubMed, Embase, and PsycINFO. The search was conducted in November 2022 with an update in October 2023. Two independent reviewers coded studies that met the inclusion criteria and assessed their methodological quality and risk of bias using the AXIS tool. Synthesis was performed by calculating frequencies and percentages. 87 articles validating 24 ICF-CSs were analyzed. Most articles showed strengths in consistency between study objectives and the outcome variables measured. However, a large majority did not report sample size calculation (up to 94.2% in Delphi studies), and few validation studies were conducted in the WHO regions of Africa and the Eastern Mediterranean. The quality of cross-sectional studies validating ICF-CSs was satisfactory, although several articles did not describe aspects such as sample size calculation. Validity evidence for ICF-CS studies could be improved by conducting more multicenter studies, replicating ICF-CS validation studies in different WHO regions, and through synthesis of existing research.

Specific leaf area and its within-individual variation in understory evergreen and deciduous woody species in New Zealand

Specific leaf area (SLA) plays a critical role in carbon assimilation and nutrient cycling. While leaf habit (deciduous vs. evergreen) has often been recognized as a reliable predictor of SLA—with deciduous species typically having higher mean SLA values due to lower concentration of structural components compared to evergreens—high variation in SLA among evergreen species suggests further investigation of variation for species with this leaf habit could improve predictions of SLA effects on community and ecosystem processes. Furthermore, the presence of leaves of different ages in evergreen plants, emerging over multiple years, could amplify the within-individual variation in SLA, which remains underexplored. Here we report variations of SLA measured from c. 2000 leaves collected from 36 individuals across 19 woody species in an understory environment in New Zealand (NZ). We found that while most deciduous species, predominantly non-native, clustered towards higher SLA values, evergreen species presented a wide SLA spectrum. Moreover, we found that while being deciduous, having a smaller leaf size, and younger leaves, collected from lateral branches, correlated with elevated SLA values, the leaf habit did not primarily drive the within-individual SLA variation. Instead, smaller leaf size emerged as a significant predictor of within-individual SLA variation. The branch-order effect on SLA underscores a methodological consideration: accurate estimation of total leaf area in evergreen trees requires representative sampling across all branch orders. Our study also highlights the need for integrating leaf traits such as leaf size and branch order into functional trait analyses. Further research is vital to understand the underlying mechanisms of these trait variations and their impacts on ecosystem functioning.

Meta-analysis of variance in tDCS effects on response inhibition

Deficiencies in response inhibition are associated with numerous mental health conditions, warranting innovative treatments. Transcranial direct current stimulation (tDCS), a non-invasive brain stimulation technique, modulates cortical excitability and has shown promise in improving response inhibition. However, tDCS effects on response inhibition often yield contradictory findings. Previous research emphasized the importance of inter-individual factors that are mostly ignored in conventional meta-analyses of mean effects. We aimed to fill this gap and promote the complementary use of the coefficient of variation ratio and standardized mean effects. The systematic literature search included single-session and sham-controlled tDCS studies utilizing stop-signal task or Go-NoGo tasks, analyzing 88 effect sizes from 53 studies. Considering the impact of inter-individual factors, we hypothesized that variances increase in the active versus sham tDCS. However, the results showed that variances between both groups did not differ. Additionally, analyzing standardized mean effects supported previous research showing an improvement in the stop-signal task but not in the Go-NoGo task following active tDCS. These findings suggest that inter-individual differences do not increase variances in response inhibition, implying that the heterogeneity cannot be attributed to higher variance in response inhibition during and after active tDCS. Furthermore, methodological considerations are crucial for tDCS efficacy.

NEW METHODOLOGIES FOR ASSESSING CAVITATION LOAD AND RESISTANCE OF STRUCTURAL MATERIALS AND PROTECTIVE COATINGS IN NUCLEAR POWER INDUSTRY. PART II. POLYFRACTIONAL APPROACH

A fractional approach to cavitation erosion process as proposed previously by one of the authors is explained. The polyfractional erosion process is considered as a superposition of a series of monofractional ones and described by a differential kinetic equation with a set of parameters constituting “the erosion resistance matrix”. The funda-mental methodological considerations are supplemented by describing the most typical applications including pre-diction of erosion of structural materials under given cavitation load, derivation of cavitation erosion fatigue charac-teristics, retrieval of effective erosive load basing on performance of reference materials. Finally, extending the methodology onto thin and thick protective coatings is proposed.

The development of a new oral health patient reported outcome measure: the New South Wales public dental services approach

BackgroundAddressing Patient Reported Outcomes (PROs) is essential for patient-centred care, shared decision making and improved health outcomes. Value-based health care systems in New South Wales (NSW) have a growing focus on collecting and using PROs that matter most to patients to improve their healthcare outcomes. Developing oral health patient reported outcomes measures (OH-PROM) is a first step towards value-based oral health care. This paper describes the development process of an adult and child OH-PROM tool that can be piloted for NSW public dental patients.MethodsAn expert panel was assembled to undertake a systematic process of developing OH-PROMs for NSW Health. Key methodological considerations included: (1) forming an expert panel to specify the target population and context of implementation, (2) rapid literature review and environmental scan to identify existing validated OH-PROM tools for adults and children. (3) consensus gathering with the expert panel (4) consumer feedback, and (5) finalisation of the tool for electronic oral health record (eOHR) integration to establish a set of questions, that were relevant, context-appropriate, and important to oral healthcare outcomes for patients using public dental services.ResultsThe panel considered a total of 59 questions from two child (15), and four adult (44) Oral Health Related Quality of Life (OHRQoL) questionnaires used to collect OH-PROMs. These questions were mapped to the four key dimensions of OHRQoL for OH-PROMs: Oral Function, Orofacial Pain, Orofacial Appearance, and Psychosocial Impact. The consensus resulted in seven questions that aligned with these four dimensions to form two new NSW OH-PROM tools: one for adults and one for children. The tools were tested with consumers for understandability and usefulness before being incorporated into the electronic oral health record system, in readiness for future pilot testing.ConclusionThe process for developing new OH-PROMs for NSW public dental services took a pragmatic approach that combined literature appraisal, expert consensus, and consumer consultation. Future work will assess the implementation of the OH-PROM tool and test its validity for broader use as an outcome measure for value-based oral healthcare.