The micro staring hyperspectral imager can simultaneously acquire two spatial and one spectral images, and only record the external orientation elements of the entire hyperspectral image rather than the external orientation elements of each frame of the image, which avoids the geometric instability during scanning, effectively solves the problem of large geometric deformation of the small line scanning hyperspectral imager, and is suitable for the small UAV load platform with unstable attitude. At present, most of the research focuses on the radio-metric correction method of line scan hyperspectral imager. The application time of staring hyperspectral imager is short, and there is no mature data processing re-search at home and abroad, which hinders the application of UAV micro staring hyperspectral imaging system. In this paper, the calibration method of the linearity and variability of the radiation response of the micro staring hyperspectral imager on the UAV is studied, and the effectiveness of this method is quantitatively evaluated. The results show that the hyperspectral image has obvious vignetting effect and strip phenomenon before the correction of radiation response variability. After the correction, the radiation response variation coefficient of pixels in different bands decreases significantly, and the vignetting effect and image strip decrease significantly. In this paper, a multi-target radiometric calibration method is proposed, and the accuracy of radiometric calibration is verified by comparing the calibrated hyperspectral image spectrum with the measured ground object spectrum of the ground spectrometer. The results show that the calibration results of the multi-target radiometric calibration method show better results, especially for the near-infrared band, and the difference with the surface reflectance measured by the spectrometer is small.