Plant Protection Methods Research Articles

Efficacy of natural and synthetic inhibitors in the elimination of viruses in potato (Solanum tuberosum L.)

Background. Developing new effective plant protection methods against viruses is a vital task for seed potato production. The use of virus inhibitors (mainly synthetic ones) is the most common method in potato virus control. Products of natural origin might be a way to reduce the dependence on synthetic inhibitors. Among the former, extracts from seaweeds have high potential, producing a low or no negative impact on the environment or human health. Materials and methods. The materials of the research included potato cvs. ‘Poseidon’ and ‘Orion’ developed at the Federal Scientific Center of Agricultural Biotechnology of the Far East named after A.K. Chaika, the commercial antiviral product Ribavirin, and the sulfated polysaccharide – carrageenan – obtained from red algae at the G.B. Elyakov Pacific Institute of Bioorganic Chemistry. A highly sensitive PCR method was employed to detect viral infections in plant samples. Results. The efficacy of chemotherapy with Ribavirin (0.03%) was established at 79.8% against PVY, 77.0% against PLRV, 80.3% against PVM, and 84.5% against PVS. The most successfully eliminated viruses were PVY, PVM, and PVS. Carrageenan as an antiviral substance eliminated potato viruses at the following rates: PLRV in 74.7% of the explants, PVM in 70.2%, PVS in 69.2%, and PVY in 16.9%. Conclusion. Analyzing the antiviral activity of natural and synthetic virus inhibitors in the case study of carrageenan and Ribavirin demonstrated their high efficacy against potato viruses. Natural polysaccharides – carrageenans – were observed to have a significant antiviral effect, so their use in agricultural biotechnology might be promising for new experiments. Our approach successfully eliminated viruses in two new promising potato cultivars, ‘Poseidon’ and ‘Orion’, which were included in the system of virus-free seed production. These cultivars were submitted for patenting and official testing under the State Variety Trials.

Эффективность производства зерна в условиях изменения климата в Зауралье

Abstract. The economic efficiency of grain production is determined by the level of return on the resources spent by the cost of the products received. Weather factors play an important role. Climate changes in the Trans-Urals are primarily associated with the warming of the spring and summer months, increased aridity of the growing season and the expansion of the boundary of this process from the southeast to the northwest. The contrast in moisture availability of different years is combined with uneven precipitation in the region throughout the year. The profitability of farms in the production of grain crops is characterized by the same heterogeneity. The purpose of the study is to establish the economic efficiency of grain production in the context of climate change using the example of the Trans-Urals. The tasks are to analyze the impact of climate change on the yield of grain crops in the region, to analyze the efficiency of resource use by agricultural enterprises; using the example of the most highly profitable farms to identify factors to increase the efficiency of grain production. The methods of monographic and statistical analysis of data from scientific literature, open statistical sources, agronomic and economic reports of agricultural enterprises were used. The scientific novelty of the research consists in studying the efficiency of grain production in the context of climate change and in relation to the volatility of grain prices and means of production. The results of the study showed that since the early 2000s, despite a significant increase in the use of fertilizers, the growth of grain yields has stopped, which is primarily due to the deterioration of plant moisture conditions. At the same time, an increase in costs, although it leads to an increase in productivity, but to a lesser extent, as a result, the cost of grain increases, and the profitability of production decreases. Enterprises have been identified that, in the conditions of climate change and high volatility of grain prices and resources, have found ways to efficiently produce grain: replace solid fertilizers with liquid fertilizers, apply effective, including agrotechnical, methods of tillage and plant protection, use highly productive varieties, obtaining the highest profitability in the region.

Optimizing the production of dsRNA biocontrols in microbial systems using multiple transcriptional terminators.

Crop pests and pathogens annually cause over $220 billion in global crop damage, with insects consuming 5%-20%of major grain crops. Current crop pest and disease control strategies rely on insecticidal and fungicidal sprays, plant genetic resistance, transgenes, and agricultural practices. Double-stranded RNA(dsRNA) is emerging as a novel sustainable method of plant protection as an alternative to traditional chemical pesticides. Successful commercialization of dsRNA-based biocontrols requires the economical production of large quantities of dsRNA combined with suitable delivery methods to ensure RNAi efficacy against the target pest. In this study, we have optimized the design of plasmid DNA constructs to produce dsRNA biocontrols in Escherichia coli, by employing a wide range of alternative synthetic transcriptional terminators before measurement of dsRNA yield. We demonstrate that a 7.8-fold increase of dsRNA was achieved using triple synthetic transcriptional terminators within a dual T7 dsRNA production system compared to the absence of transcriptional terminators. Moreover, our data demonstratethat batch fermentation production dsRNA using multiple transcriptional terminators is scalable and generates significantly higher yields of dsRNA generated in the absence of transcriptional terminators at both small-scale batch culture and large-scale fermentation. In addition, we show that application of these dsRNA biocontrols expressed in E. coli cells results in increased insect mortality. Finally, novel mass spectrometry analysis was performed to determine the precise sites of transcriptional termination at the different transcriptional terminators providing important further mechanistic insight.

Deciphering the key factors affecting pesticide residue risk in vegetable ecosystem

Soil contamination, particularly from pesticide residues, presents a significant challenge to the sustainable development of agricultural ecosystems. Identifying the key factors influencing soil pesticide residue risk and implementing effective measures to mitigate their risks at the source are essential. Here, we collected soil samples and conducted a comprehensive survey among local farmers in the Three Gorges Reserve Area, a major agricultural production region in Southwest China. Subsequently, employing a dual analytical approach combining structural equation modeling (SEM) and random forest modeling (RFM), we examined the effects of various factors on pesticide residue accumulation in vegetable ecosystems. Our SEM analysis revealed that soil characteristics (path coefficient 0.85) and cultivation factor (path coefficient 0.84) had the most significant effect on pesticide residue risk, while the farmer factors indirectly influenced pesticide residues by impacting both cultivation factors and soil characteristics. Further exploration using RFM identified the three most influential factors contributing to pesticide residue risk as cation exchange capacity (CEC) (account for 18.84%), cultivation area (account for 14.12%), and clay content (account for 13.01%). Based on these findings, we carried out experimental trials utilizing Integrated Pest Management (IPM) technology, resulting in a significant reduction in soil pesticide residues and notable improvements in crop yields. Therefore, it is recommended that governmental efforts should prioritize enhanced training for vegetable farmers, promotion of eco-friendly plant protection methods, and regulation of agricultural environments to ensure sustainable development.

Устойчивость горчицы черной к фузариозному увяданию в условиях центральной зоны Краснодарского края

The different diseases decreasing quantitative and qualitative yield indicators can infected black mustard as the other oil crops of Brassicaceae family. Fusarium wilt is one of the most dangerous diseases for the crop. If plants are infected with disease pathogens (fungi Fusarium spp.) in a phase of 2– 4 true leaves root rot developes of young plants that causes their depth. In adult plants, conductive bundles in stems become clogged, causing premature drying of plants. Cultivation of resistant varieties is economically profitable and environmentally safe method of plant protection. The purpose of the research was to estimate new breeding material of black mustard on resistance to fusarium wilt in the central zone of the Krasnodar region. In a phase of yellow-green pod, breeding material of black mustard was estimated on resistance to the disease in a form of tracheomic wilting using 10-point scale of sample infection degree: from 0 to 9 points. All samples of mustard were divided on five group: immune, resistant, weak resistant, weak susceptible, and susceptible. During three years of research, the immune samples of black mustard were not selected. Ten samples of the crop were selected, they were resistant to the fusarium wilting on middle and high natural infection background in all years of the research. It allows recommending them to use in breeding of new black mustard varieties.

“BioClay™”: One Step toward the Sustainable and Novel Plant Protection Method

“BioClay™”: One Step toward the Sustainable and Novel Plant Protection Method

Preliminary Tests of Tomato Plant Protection Method with Ozone Gas Fumigation Supported with Hydrogen Peroxide Solution and Its Effect on Some Fruit Parameters

The aim of this research was to determine the impact of hydrogen peroxide spraying and ozone gas fumigation during the growing season of tomato plants grown under cover on the mechanical and chemical parameters of fruit harvested from these plants. Tomato plants were grown under cover in accordance with the principles of good agricultural practice in the soil and climatic conditions of southeastern Poland. During the growing season, tomato fruits were collected for testing in order to determine the impact of the applied variable factors on the modification of selected metabolic pathways of bioactive compounds. As part of the tests on the chemical properties of the fruits, the content of ascorbic acid, the total content of polyphenols, and the antioxidant potential were determined. Additionally, the influence of the tested variable factors on the mechanical properties of tomato fruits was determined. In the case of the total polyphenol content, the most beneficial effects were observed for fruits collected from plants treated with ozonation at a dose of 2 ppm for 3 min and spraying the plants with 1% hydrogen peroxide. The highest antioxidant potential was recorded for fruits of the variants ozonated with doses of 2 ppm for 1 min, 2 ppm for 1.5 min, and 2 ppm for 3 min compared to the remaining variants and controls. In turn, the vitamin C content increased significantly in the tested fruits after the ozonation of plants with a dose of 2 ppm for 1 min and ozonation with a dose of 2 ppm for 3 min combined with spraying plants with 3% hydrogen peroxide. In the case of the mechanical properties of tomato fruits, only the ozonation dose of 2 ppm for 3 min significantly improved them.

Evaluation of phytoconstituents in marigold effluent for their antifungal activity against plant pathogens.

The current study placed an intense emphasis on the excess discharge of agro-based industrial effluent and the use of plant extract antimicrobials to inhibit the growth of pathogens in crop plants. An effluent (treated and untreated) from the marigold flower processing industry has been identified for the presence of volatile and semi-volatile organic compounds, and a total of 18 in treated effluent and 23 in untreated effluent were found using gas chromatography-mass spectrometry. A total of 13 classes were identified, which include carboxylic acid, phenols, esters, alkanes, alkenes, alcohols, cyanide, heterocyclic, flavonoids, aldehydes, polycyclic aromatic, cycloalkanes, and cycloalkenes. A principal component analysis with varimax rotation was applied to discern the abundance of identified compounds under each class. An in vitro antifungal bioassay was conducted using effluents at three different concentrations against plant pathogens (Alternaria alter nata, Sclerotium rolfsii, Rhizoctonia solani, Pythium aphanidermata, Fusarium oxysporum, and Colletotrichum gloeosporioides). The study proved that treated and untreated effluents clearly inhibited the growth of fungal pathogens by 10 to 32% and 37 to 92%, respectively. The findings suggest that marigold flower effluent can be a promising resource for developing new plant protection methods that are effective against pathogenic fungi.

Landscape design and planning methods for plant protection based on deep learning and remote sensing techniques

Landscape design and planning can play a role in beautifying the human living environment while improving the ecological protection of plants. However, it is difficult because it involves ornamental, ecological, social and other factors. Remote sensing data can be acquired and analyzed from vegetation distribution, ecological environment, land use and other information to support rational planning and design of plant protection landscape. Deep learning technology provides a powerful tool for the comprehensive use of remote sensing data to support plant protection landscape design. In this paper, a multiple dense network (MP-DenseNet) model is constructed based on Densenet to accomplish the task of classifying the vegetation cover of remotely sensed data, which provides data support for the design and planning of plant protection landscapes. The algorithm proposed in this paper is competitive in comparison with several models. Using the MP-DenseNet model, this paper categorizes the vegetation cover of the study area and proposes an overall planning scheme that balances plant protection and regional sustainable development.

Benefiting from Complexity: Exploring Enhanced Biological Control Effectiveness via the Simultaneous Use of Various Methods for Combating Pest Pressure in Agriculture

Biological control, a well-established plant protection method, has garnered substantial attention in recent decades. Various approaches, including biological control agents (BCA), catch crops, biofumigation, sticky traps, and pheromones, have been extensively explored. While the effectiveness of these methods varies depending on specific circumstances, their collective significance has grown amid mounting pressures to curtail or eliminate conventional synthetic plant protection products. Previous review articles have highlighted the benefits of using two or more BCAs simultaneously, yet limited information exists regarding the concurrent use of diverse biological control methods. This comprehensive review incorporates a thorough literature search to assess the benefit of concurrently employing two or more of these methods, followed by a discussion on perspectives of holistic management and mimicking complex natural systems, shedding light on the vast potential and need for further research in this domain.

Protecting spring wheat plants using selenium and pesticides.

The research was carried out in order to find ways to optimize the system of protection of spring wheat crops. In the conducted studies, the effect of combinations of sodium selenite and various pesticides, differing in the specifics of action and biological activity, on the yield and quality of spring wheat of the Yubileinaya 80 variety was studied. Currently, there is a need to achieve a sufficient effect of the action of chemical plant protection products and to obtain a minimum impact on human health and the environment. The purpose of the research is to study the influence of various combinations of chemical plant protection products and methods of using sodium selenite on the yield and grain quality indicators of spring wheat variety Yubileinaya 80. The studies were carried out under the conditions of a vegetative experiment with spring wheat variety Yubileinaya 80. Two methods of using sodium selenite and chemical plant protection agents of different specifics of action were studied: fungicide, herbicide, and insecticide, which were applied in different combinations and at different times. As a result of the studies, the phytotoxicity of the studied preparations of chemical plant protection was revealed, which apparently manifests itself as a result of inhibition of the morphometric indicators of the growth of the root system and vegetative organs of wheat plants, resulting in a violation of the processes of accumulation of assimilates and their outflow to the reproductive organs. Optimal combinations of pesticides and sodium selenite have been established, allowing to obtain reliable changes in yield and quality indicators of wheat grain. It was revealed that the use of selenium treatment before sowing seeds contributed to a decrease in the phytotoxicity of the studied pesticides, as a result of stimulating the processes of absorption by plants and the redistribution of nitrogen to the reproductive organs of wheat, which had a positive effect not only on the yield and quality of spring wheat of the Yubileynaya 80 variety, but also on the external surface microstructure of the fruit shell of the grain. The noted features of the surface of the fruit shell of the grain will reduce losses during grain processing and obtain processed products from such grain of higher quality.

Mechanical and biological methods of plant protection against agrophages in the context of the European Green Deal strategy

The intensive development of agriculture in Europe has caused that all European Union member states are currently increasingly expe¬riencing negative consequences such as depletion of natural resources, increased erosion and loss of natural soil fertility, increased importance of new diseases and pests, water shortages, reduced biomass production and decreased biodiversity of organisms. In the context of the common agricultural policy, as well as the need to limit the use of chemical protection of agricultural crops, agrotechnical and also mechanical and biological methods are gaining in importance. Mechanical methods of protecting plants against pests main¬ly consist in mechanical destruction of threats or preventing them from reaching plants in the field or agricultural products stored in storage. Biological methods, on the other hand, use viruses, microorganisms and macroorganisms to combat pests as an alternative to chemical plant protection. This publication presents available mechanical and biological methods that allow for minimizing the use of chemicals in agriculture.

ОПРЕДЕЛЕНИЕ ЭНЕРГЕТИЧЕСКОЙ ЭФФЕКТИВНОСТИ НА СТАДИИ ПРОЕКТИРОВАНИЯ СИСТЕМ ЗАЩИТЫ СЕЛЬСКОХОЗЯЙСТВЕННОГО ПРОИЗВОДСТВА ОТ НАСЕКОМЫХ-ВРЕДИТЕЛЕЙ

The food program provides for the intensification of all branches of agriculture. The task has been set to increase the yield of grain crops in an amount that fully meets the needs of the population and the processing industry. The article discusses the advantages and disadvantages of various methods of protecting plants from insect pests. The trends in the development of an integrated method of plant protection, contributing to the improvement of environmental safety, are considered. An algorithm for evaluating engineering solutions at the design stage of a system of protective measures using the finite ratio method is considered. The expediency of using an integrated approach to design to increase energy efficiency in agricultural enterprises is proved. Recommendations for the protection of crops from pests and diseases are combined under the general name of a system of measures that include various methods of control - biological, chemical, agrotechnical, mechanical and integrated. Pest and disease control occupies an important place among the measures ensuring the preservation and improvement of crop yields. The success of this work depends on the timely implementation of protective measures in combination with preventive and agrotechnical methods of plant care. There are several basic methods of carrying out protective measures in the plant protection system: biological method, chemical method, agrotechnical method, mechanical method, integrated method. Currently, prerequisites have been created for the creation of autonomous monitoring devices due to the appearance of new light sources on the domestic market – LEDs, the main advantages of which are: low power consumption, high luminous efficiency, long service life, high mechanical strength, reliability and safety.

Assessing Agricultural Practices and Plant Protection Methods in Rupnagar District, Punjab, India

Aims: The purpose of this research is to analyze plant protection systems and agricultural practices in Punjab, India's Rupnagar District, in order to provide insights into nutrient management, seed rates, crop diversity, mechanization, and their implications.
 Conclusion: The study emphasizes the significance of improved mechanization, crop diversity, and sustainable farming techniques in Rupnagar, Punjab, to promote future agricultural development.
 Methodology: The study employed a mixed-methods approach, combining field surveys and structured interviews with 120 farmers across six villages in Rupnagar District, Punjab, India. Data on crop types, seed rates, machinery usage, and nutrient management were collected. Random sampling ensured representativeness, providing comprehensive insights into agricultural practices and challenges in the region.
 Place and Duration of Study: Villages of Rupnagar district, Punjab (Primary place) and Department of Agriculture sciences, Chandigarh university, Gharuan (Secondary place), between August 2023 and September 2023
 Results: According to the data, the two main crops grown in the research areas were rice and wheat. Among the various degrees of mechanization observed, tractors were the most frequently visible piece of equipment. Seed rate management varied, and some farmers used more seed than was recommended. The data revealed a diversity of nutrient management approaches, as well as a proclivity for urea overuse. These findings offer insight on how complex the farming methods in the area are.
 Study Design: A mixed-methods methodology is used in the study design. It combines field surveys, interviews, and data analysis to extensively analyze agricultural practices in Rupnagar District, Punjab, India, including crop diversification, mechanization, and the use of plant protection strategies.

Study of grape microorganisms in Moldovan vineyards: influence of human and natural factors

The objective of this study was to investigate the microbial diversity on the grape berries under different climate conditions. Sixteen samples from ten vineyards were taken from three PGI regions in Republic of Moldova Moldova during years 2018–2020. The year of harvest had a significant effect on the amount of yeasts, but the geographical location did not significantly influence this criterion. As natural factors, the influence of temperature and precipitation during both the growing season and the month of grape harvest was studied. The selected methods for vineyard plant protection were as follows: fungicides to control powdery mildew, fungicides to control mange and insecticides. The total yeast population per grape ranged from 3.8E+04 to 2.3E+07 CFU/berry and the culturable yeast population ranged from 1.7E+03 to 8.0E+06. The harvest month temperature affects the yeast population the most, fungicide use in mealybug control reduces the culturable yeast population but increases the total yeast population; insecticide treatment only increases the total yeast population but has no impact on culturable yeasts.In addition, six species yeast have been identified: A.pullulans, M.pulcherrima, H. uvarum, R. Glutinmis, R. graminis and S.cerevisiae. The Saccharomyces cerevisiae strain isolated from the 2020 crop is totally different from the 2018 and 2019 crops. The bacteria population is at a low level, except for a few samples, P. damnosus, L. brevis, L.fermentum, G. oxydants were isolated.

A Review: Development of Plant Protection Methods and Advances in Pesticide Application Technology in Agro-Forestry Production

In this review, through reviewing the history of the struggle between human beings and plant diseases, insects and weeds, more specifically thoughts on plant protection in ancient Chinese agricultural books, the recognition of plant pests as a target and six types of plant protection methods and 36 subdivision measures are summarized. Then, we focus on the development overview of pesticide application technology and conduct a systematic review by combining the development timeline of pesticide application and key technologies including performance measurement and the simulation and modeling of pesticide-spraying systems. Finally, three suggestions for further research are proposed from the perspectives of human beings’ and environmental health, sustainable and eco-friendly application media and efficient application equipment systems in plant protection.

Environmental risks due to the impact of pesticide load in agrocenoses of cereal crop predecessors

The article is dedicated to the study of the impact of pesticide load on agrocenoses of cereal crop predecessors and the emergence of ecological risks in them. An assessment of ecological risks in the agrocenoses of the experimental field of the State Enterprise “Research Farm “Skvyrske” of IAEM of NAAS” was carried out using pesticides during the growing seasons 2021–2023. The methodology for assessing ecological risks of potential pesticide pollution of agrocenoses by indicators of agroecotoxicological index (AETI) was used. It was determined that the level of ecological risk from pesticide pressure in the crop rotation of the experimental field by AETI is within the range from 2.86·10–3 to 2.24·10–1 сonventional units and is characterized as slightly dangerous, which indicates a minimal ecological risk of pesticide use. The obtained results are a sign that the load of applied pesticides on the agrocenoses of the experimental farm corresponds to the territory’s ability to self-clean. It is shown that rational use of chemical methods of plant protection in combating pests, disease pathogens and weeds creates prerequisites for obtaining environmentally safe grain and seed products, as well as minimizes the likelihood of environmental of grain and seed contamination with pesticides of cultivated crops. Usually, longterm and repeated use of pesticides in any case leads to accumulation of their residues or metabolites in the soil and, as a result, is a source of contamination of crop products and environmental objects. Therefore, using the results of assessing pesticide load on agroecosystems, taking into account the territory’s ability to self-clean, can be considered as one of the levers for managing environmental risks. To minimize ecological risks from pesticide pollution of agrocenoses and increase environmental safety of agroecosystems, measures should be taken to regulate the use of chemical plant protection products. This should be done by banning or restricting the use of pesticides that have a high level of ecotoxicity and are persistent in soil.

Examining Modern Strategies for Effective and Sustainable Agricultural Plant Protection Techniques: A Review

The advancement of plant protection strategies is integral to sustainable agriculture, food security, and ecological balance. While modern approaches i.e. chemical, biological, and technological - have contributed significantly to plant protection, they come with their own sets of challenges and limitations. Chemical methods, potent in their action, often result in environmental degradation, bioaccumulation of toxic substances, and the onset of resistance among pests. Biological approaches, although aligned with ecological principles, face difficulties related to scalability, variable effectiveness, and dependency on environmental conditions. On the technological front, innovative solutions such as drones, precision agriculture, and data analytics promise transformative change but are constrained by factors like high setup costs and technical expertise. Despite the achievements, there exist notable research gaps, especially concerning the long-term sustainability of these methods. Comprehensive studies are often lacking that holistically assess the social, economic, and environmental aspects of plant protection techniques. This article aims to provide an in-depth analysis of the limitations of current strategies, identify existing research gaps, and suggest future prospects for making plant protection more efficient and sustainable. Areas for future research include the development of nano-pesticides for more targeted and eco-friendly applications, and the incorporation of adaptive methods to address challenges presented by climate change. The paper concludes that a multidisciplinary research approach is essential for overcoming existing challenges and for the development of more effective, sustainable plant protection strategies. Through an exhaustive review of current literature and case studies, this article serves as a comprehensive guide for researchers, policymakers, and agricultural practitioners to navigate the complex landscape of modern plant protection methods, aiming to provoke thought and inspire action towards more sustainable solutions.

Economic Development and Pesticide Use in EU Agriculture: A Nonlinear Panel Data Autoregressive Distributed Lag Approach

Within the regime established by the Directive on Sustainable Use of Pesticides (SUDP); the present work explores the relationship between pesticides’ agricultural use per hectare of cropland and the GDP per capita of the rural population for twenty-five EU countries to unveil the efficiency of the current EU strategy. With the econometric tool of panel nonlinear autoregressive distributed lag (NARDL) cointegration technique; we try to capture potential asymmetries in the agricultural use of pesticides concerning positive and negative variations in agricultural income. The findings validate the existence of a long-run relationship that supports an Environmental Kuznets Curve (EKC); i.e., an inverted U-shaped relationship between the variables; since increasing agricultural income is related to reductions in the use of pesticides after the turning point. Even though this result is not validated in the short run; our findings confirm the existence of a steady-state situation with asymmetric responses to pesticides. In terms of policy implications; more measures need to be taken; along with the education of farmers; aiming to enhance their consciousness towards environmental issues and; in consequence; for them to prefer environmentally friendly plant protection methods over chemical ones.

Effect of pre-sowing seed treatment with biologics on the spring barley (Hordeum vulgare L.) yield in the Lower Volga region of Russia

The biologization of crop production involves reducing the use of synthetic organic pesticides in the production cycle of harvesting, increasing the use of biological methods of plant protection that solve the problems of environmental pollution and agricultural products. The basis for the biologization of plant protection systems is the wider use of biological products that make it possible to obtain environmentally friendly, economically sound yields of major agricultural crops. In this regard, studies were conducted in the arid zone of the Lower Volga region, Federal Scientific Center for Agroecology, Russia from 2018 to 2020 to study the influence of pre-sowing seed treatment with chemical, biological protectants and physiologically active substances as well as an economic assessment of the impact of this agricultural to increase the yield of spring barley. The objects of research in field experiments were chemical seed protectant (Inshur Perform, CS), biological fungicide (BSKa-3), and organic fertilizers (Fertigrain Start, BP and potassium humate, BP). The maximum values for such indicators as field germination – 86%, plant survival for harvesting – 61%, productive bushiness – 1.04% were recorded in the variant of the use of biological preparations. There was also the highest number of grains in the ear – 14.5 pcs., the mass of 1000 seeds – 32.1 g. In comparison with the yield with the variants without treatment (control) and with the use of only chemical preparations, the yield in the variant with the use of biological preparations was, respectively, 25.7% more.