The article studies the safety of sterlet grown in recirculating aquaculture systems. The research was carried out in the research and training laboratory on reproduction and cultivation of sturgeon species of the Department of private animal husbandry, livestock and aquaculture technology of Ulyanovsk State Agrarian University named after P. A. Stolypin and Simbirsk Veterinary Reference Center in Ulyanovsk. The material for the study was chosen sterlet grown in recirculating aquaculture systems. In the recirculating aquaculture system food components and metabolites accumulate, which may affect fish health and quality and safety of fish meat. In the course of studies it has been found that hydrochemical parameters of water taken from the breeding tanks comply with the standards: ammonia content as the main indicator affecting the growth and development of fish did not exceed 0.05 mg/dm3. This is due to compliance with fish stocking density, optimum temperature (18-22°C) and pH level (7.3). The content of sulfates was also within the norm -52.78 mg/dm3. Mass fraction of chlorides was 14.82 mg/dm3 and of nitrites - 0.052 mg/dm3, which is 20 and 50 times lower than the standard values. The fish was studied for organoleptic, microbiological parameters, as well as for the content of toxic elements. Organoleptic characteristics (appearance, fish condition, smell, color of eyes and gills) fully meet the requirements of GOST 7631-2008 “Fish, non-fish objects and their products. Methods for determination of organoleptic and physical parameters”; such toxic elements as cadmium (Cd), arsenic (As), mercury (Hg) and lead (Pb) were detected in fish samples, but their content was negligible and did not exceed the maximum permissible concentrations. The presence of genetically modified organisms, pathogens, BGB (coliforms), Listeria, nematodes were not detected. According to the study results, sterlet grown in the recirculating aquaculture conditions of the research laboratory for reproduction and breeding of sturgeon species of the Department of private zootechny, technology of animal husbandry and aquaculture of Ulyanovsk State Agrarian University can be considered completely safe in terms of microbiological, parasitological and chemical indicators.