Athletes are exposed to acute and chronic stress that may lead to suppression of the immune system and increased oxidative species generation. Normal Fe and Zn levels are essential for the development and maintenance of immune functions and antioxidative system for athletes. This study evaluated the effect of Fe and Zn combinative supplementation on the immune function in female athletes with oxidative stress. The experiment group was supplemented with Zn and Fe as Zn glyconate and Fe‐glycerite, respectively, for 2 month. Before and after supplementation, they exercised in treadmill until exertion according to Bruce protocol. The serum concentrations of Fe and Zn were measured by neutron activation analysis method. The hemoglobin levels were not changed in supplemented athletes in contrast to significant decline in placebo athletes. Declines of resting serum Zn and Fe level after 2‐mo. period in athletes were diminished by Zn and Fe supplementation. The reduction of serum Zn levels after exercise was diminished in athletes with Zn and Fe supplements compared to the placebo group. The decline of basophile proportion was significantly diminished by 2‐mo supplementation (p<0.05). However, the increase of PHA‐induced lymphocyte proliferation was lowered by Fe and Zn supplementation in resting state in contrast to increase of ConA‐induced lymphocyte proliferation after exercise.