Laser-induced Fluorescence Method Research Articles

Development of a DualEmission Laser-Induced Fluorescence (DELIF) Method for Long-Term Temperature Measurements

The fluorescence intensity of fluorescent dyes typically employed in the dual-emission laser-induced fluorescence (DELIF) method gradually degrades as the excitation time increases, and the degradation rate depends on the type of fluorescent dye used. Therefore, the DELIF method is unsuitable for long-term temperature measurements. In this study, we focused on the fluorescence intensity ratio of a single fluorescent dye at two fluorescence wavelengths and developed a DELIF method for long-term temperature measurements based on this ratio. The fluorescence intensity characteristics of Fluorescein disodium and Rhodamine B, which are typically used in the DELIF method, in the temperature range of 10–60 °C were comprehensively investigated using two high-speed monochrome complementary metal-oxide semiconductor cameras and narrow bandpass filters. Interestingly, the ratio of the fluorescence intensity of each fluorescent dye at the peak emission wavelength of the fluorescence spectrum, λ, to the fluorescence intensity at wavelengths very close to the peak wavelength (λ ± 10 nm) was highly sensitive to temperature variations but not excitation time. Particularly, when Rhodamine B was used, the selection of the fluorescence intensity ratios at a wavelength combination of 589 and 600 nm enabled highly accurate temperature measurements with a temperature resolution of ≤0.042 °C. Moreover, the fluorescence intensity ratio varied negligibly throughout the excitation time of 180 min, with a measurement uncertainty (95% confidence interval) of 0.045 °C at 20 °C. The results demonstrate that the proposed DELIF method enables highly accurate long-term temperature measurements.

Investigation of Water Film Dynamics on the Surface of an Airfoil in a High-Speed Flow and Subsequent Ligament Formation and Breakup from the Trailing Edge

Abstract In this work, the dynamics of a liquid film on the surface of NACA 0012 airfoil placed in a high-speed air flow is investigated. Experimental studies were carried out to assess the film thickness, droplet shedding, and the dynamics of the sheet. In the present work, air velocities up to 175 m/s were used with water films flowing between 1.4 and 2.6 cm2/s. The water was introduced on the airfoil via 26 holes each measuring of 0.5 mm holes separated by 1mm at 35 mm downstream of the leading edge of the vane. The results were obtained using four experimental tools. The first is a point measurement of the time-resolved film thickness using a confocal laser induced fluorescence method. Second is time averaged images of the film thickness on the entire vane surface. Third, high speed videos are obtained to study the accumulation and breakup of the liquid at the trailing edge of vane. Finally, laser diffraction and Phase Doppler interferometry were used to document the spray dynamics. The results illustrate that the average film thickness decreases with air velocity and increases with the water flow rate. Also, the dominant frequency of liquid film wave, ligament breakup length, drop size and spray concentration increase with the air velocity and is modestly affected by water flow rate. Finally, design tools are provided to predict the average film thickness and dominant frequencies of the film thickness, ligament breakup, spray concentration and droplet average size.

Capillary Electrophoresis-Laser Induced Fluorescence Method Development and Validation for Quantification of Nine Gangliosides-Application to Analysis of Cell Lines of CNS Origin.

Gangliosides are sialic acid-containing glycosphingolipids that play an essential role in many biological and pathophysiological processes. They are present in high amounts in the central nervous system and their abnormal metabolism or expression has been observed in many diseases. We have developed and validated a sensitive capillary electrophoresis laser-induced fluorescence (CE-LIF) method for the separation and quantification of oligosaccharides digested from nine gangliosides of high biological relevance. APTS was used for the labeling of the glycans. Reverse polarity CE was performed for the separation of the labeled glycans bearing negative charges. The optimized background electrolyte is a 15 mM lithium acetate buffer with pH of 5 containing 5% w/v linear polyacrylamide, which allows for the separation of all nine gangliosides. Validation parameters including linearity, precision, and accuracy were evaluated. LOQ and LOD were in the nM range, comparable to those of LC-MS techniques. The method was used to identify and quantify the ganglioside pattern of glioblastoma and neuroblastoma cell lines. The presented method is a valuable tool for further investigations aiming at understanding the role of gangliosides in various neurological diseases or CNS tumors.

Effect Of Wavy Structure Of Liquid Film On Flow Characteristics Of Impingement Jet Flowing On Fuel Liquid Film

In recent years, diesel engines have been downsized in order to improve combustion efficiency by using high-pressure injection to atomize the fuel and to increase fuel economy. As a result, the diesel spray flame inevitably impinges on the combustion chamber wall, resulting in heat loss due to heat transfer between the flame and the wall. According to Newton's cooling law, the heat transferred from the flame to the wall is proportional to the heat transfer coefficient, which is closely related to the flow of the spray flame. However, there are few reports on the flow of a spray flame impinging on a wall. The flow characteristics of a non-vaporized spray impinging on a wall surface, assuming that the flow of a spray flame impinging on a wall surface is equivalent to that of a non-vaporized spray. The fuel film formed on the wall affected on the flow characteristics of the spray. When the spray flame impinges on the wall in the engine cylinder, no liquid film is formed on the wall. Still, during cold start, a liquid fuel film may form on the piston wall due to the low temperature in the engine cylinder, resulting in HC and soot emissions. As a result, the spray may flow over the formed film. Therefore, it is necessary to investigate the flow characteristics of the spray flowing over the fuel film. In this study, the experiments using an impingement gas jet were conducted on a wall surface with a fuel film, and the velocity field of the jet after impingement of the wall surface was measured by the time-series PIV. In addition, the thickness of the wavy liquid film formed by the gas jet impingement was measured by using the laser-induced fluorescence (LIF) method, discussing the relationship between the velocity of the jet after wall impingement and the characteristics of the wavy liquid film.

Insight in NO synthesis in a gliding arc plasma via gas temperature and density mapping by laser-induced fluorescence

A gliding arc (GA) plasma, operating at atmospheric pressure in a gas mixture of 50% N2 and 50% O2, is studied using laser-induced fluorescence spectroscopy. The main goal is to determine the two-dimensional distribution of both the gas temperature and the NO ground state density in the afterglow. As GA plasma discharges at atmospheric pressure normally produce rather high NO x densities, the high concentration of relevant absorbers, such as NO, may impose essential restrictions for the use of ‘classical’ laser-induced fluorescence methods (dealing with excitation in the bandhead vicinity), as the laser beam would be strongly absorbed along its propagation in the afterglow. Since this was indeed the case for the studied discharge, an approach dealing with laser-based excitation of separate rotational lines is proposed. In this case, due to a non-saturated absorption regime, simultaneous and reliable measurements of both the NO density and the gas temperature (using a reference fitting spectrum) are possible. The proposed method is applied to provide a two-dimensional map for both the NO density and the gas temperature at different plasma conditions. The results show that the input gas flow rate strongly alters the plasma shape, which appears as an elongated column at low input gas flow rate and spreads laterally as the flow rate increases. Finally, based on temperature map analysis, a clear correlation between the gas temperature and NO concentration is found. The proposed method may be interesting for the plasma-chemical analysis of discharges with high molecular production yields, where knowledge of both molecular concentration and gas temperature is required.

Investigation on mixing performance of the mini-fluidized bed

Mini-fluidized beds (MFBs) can significantly enhance the mass transfer, heat transfer and mixing process. In this study, planar laser induced fluorescence method (PLIF) was used to evaluate the mixing performance in liquid-solid mini-fluidized beds. In contrast to the particle-free tubes, the relative mixing index, mixing length, mixing time, specific power consumption, mixing effectiveness and energy efficiency of mini-fluidized beds with inner diameters of 1–3 mm were analyzed. The relative mixing index of the mini-fluidized beds is 3.07–9.55 times that of the particle-free tubes under the same conditions, and the mixing length and time are reduced by 45.11% ∼ 99.59%. When the bed-to-particle diameter ratio is 13.86, the optimal operating voidage is about 0.76, which corresponds to the maximum mixing effectiveness and mixing energy efficiency. The mixing enhancement of the mini-fluidized bed system was evaluated, which provides a theoretical basis for the new application of microstructures in mini-fluidized bed reactors.

Reaction Kinetics of CH2OO and syn-CH3CHOO Criegee Intermediates with Acetaldehyde.

Criegee intermediates exert a crucial influence on atmospheric chemistry, functioning as powerful oxidants that facilitate the degradation of pollutants, and understanding their reaction kinetics is essential for accurate atmospheric modeling. In this study, the kinetics of CH2OO and syn-CH3CHOO reactions with acetaldehyde (CH3CHO) were investigated using a flash photolysis reaction tube coupled with the OH laser-induced fluorescence (LIF) method. The experimental results indicate that the reaction of syn-CH3CHOO with CH3CHO is independent of pressure in the range of 5-50 Torr when using Ar as the bath gas. However, the rate coefficient for the reaction between CH2OO and CH3CHO at 5.5 Torr was found to be lower compared to the near-constant values observed between 10 and 100 Torr. Furthermore, the reaction of syn-CH3CHOO with CH3CHO demonstrated positive temperature dependence from 283 to 330 K, with a rate coefficient of (2.11 ± 0.45) × 10-13 cm3 molecule-1 s-1 at 298 K. The activation energy and pre-exponential factor derived from the Arrhenius plot for this reaction were determined to be 2.32 ± 0.49 kcal mol-1 and (1.66 ± 0.61) × 10-11 cm3 molecule-1 s-1, respectively. In comparison, the reaction of CH2OO with CH3CHO exhibited negative temperature dependence, with a rate coefficient of (2.16 ± 0.39) × 10-12 cm3 molecule-1 s-1 at 100 Torr and 298 K and an activation energy and a pre-exponential factor of -1.73 ± 0.31 kcal mol-1 and (1.15 ± 0.21) × 10-13 cm3 molecule-1 s-1, respectively, over the temperature range of 280-333 K.

Quantitative estimation of ·OH-radical density from fluorescence decay behavior near the plasma-liquid interface by incident microscopic LIF spectroscopy

The formation and transportation of short-lived species on/within the plasma-liquid interfacial layer plays a crucial role in various applications because of their high chemical reactivity. However, the experimental detection and quantification of these short-lived species, such as ˙OH radicals, at the gas–liquid interface still pose formidable challenges. This study confronts this challenge by employing incident microscopic laser-induced fluorescence (mLIF) method to capture the OH-LIF signals on the interfacial layer at different time moments of the post-discharge phase under high spatial resolution. The temporal evolution of ˙OH density is subsequently quantified by fitting the OH-LIF decay behavior to a reaction-dissolution model. Results reveal that increasing the pulse width serves better to enhance ˙OH generation on liquid surface, reaching a density of 1.25 × 1016 cm−3. Furthermore, the cathode-solution interface demonstrates significantly enhanced ˙OH production compared to the anode-solution interface. These results underscore the efficacy of incident mLIF in quantitatively probing short-lived ˙OH-radical production at the interfacial layer in pulsed-driven plasma-solution interactions, with potential applicability to other reactive species.

Advancements in laser-based spatiotemporal measurements of flow boiling

Recent advancements in laser and imaging systems, as well as in computational processing capabilities, have made quantitative optical imaging, which is often combined with laser illumination, highly adaptable, robust and reliable. Laser-based diagnostic techniques, such as planar laser-induced fluorescence (PLIF), offer the possibility of simultaneous spatiotemporally resolved measurements of temperature fields in the liquid phase at boiling conditions. In this paper, we examine the applicability of two-colour PLIF (2cPLIF), where the ratio between individual fluorescent emissions from uniformly dispersed dyes is used to take temperature measurements in the liquid phase in the presence of moving vapour-liquid interfaces typical of boiling flow. The implementation of 2cPLIF necessitates uniformity in the concentration of different dyes across the flow field. However, in the case of a multiphase flow such as boiling, thermophoresis can lead to inhomogeneous dye distributions. To overcome this challenge, a single-dye multispectral planar laser-induced fluorescence (SDMS-PLIF) method has been developed, which employs fluorescent emissions in different spectral bands of the same dye (Nile Red). The spectral characteristics of Nile Red were measured using a spectrometer to identify its temperature-sensitive bands over a wide range of dye concentrations, from 0.3 to 30 mg/L. Following this, we demonstrate the measurement capabilities of SDMS-PLIF thermography as applied to a boiling flow in a miniaturised vertical square channel, gaining insight into the thermohydrodynamic interactions between vapour bubbles and a heated wall.

Assessing the efficacy of an atmospheric air DC glow discharge system for sustainable nitrogen fixation: a vibrational coherent anti-Stokes Raman scattering study

In addressing the substantial greenhouse gas emissions produced by the energy-intensive Haber–Bosch synthesis, this study investigates the viability of sustainable nitrogen fixation (NF) via low-temperature plasma systems energized by renewable sources. Utilizing vibrational coherent anti-Stokes Raman scattering as a diagnostic tool, we probed the nitrogen rovibrational temperature and population dynamics within a DC glow discharge in atmospheric air, a setting with considerable promise for eco-friendly fertilizer production. Besides, density for atomic N , O , and NO was quantified by laser-induced fluorescence (LIF) or two-photon absorbed LIF methods. Our findings reveal a quasi-equilibrium between rotational and vibrational energy states in the DC glow discharge environment, reaching an approximate value of 3500 K at the discharge core. The discharge parameters, discharge current, air flow rate, and discharge gap influence the rovibrational temperature, density distribution of species of interest, and the NF energy cost. However, the influences induced by these parameters are of limitations. Further analysis implies that the high gas temperature and its induced significant vibrational-rotational and vibrational-translational energy exchange are mainly responsible for the non-ideal NF energy cost.

Effect of gas components on the post-discharge temporal behavior of OH and O of a non-equilibrium atmospheric pressure plasma driven by nanosecond voltage pulses

OH radicals and O atoms are two of the most important reactive species of non-equilibrium atmospheric pressure plasma (NAPP), which plays an important role in applications such as plasma medicine. However, experimental studies on how the gas content affects the post-discharge temporal evolutions of OH and O in the noble gas ns-NAPP are very limited. In this work, the effect of the percentages of O2, N2, and H2O on the amounts of OH and O productions and their post-discharge temporal behaviors in ns-NAPP is investigated by laser-induced fluorescence (LIF) method. The results show that the productions of OH and O increase and then decrease with the increase of O2 percentage. Both OH and O densities reach their maximum when about 0.8% O2 is added. Further increase of the O2 concentration results in a decrease of the initial densities of both OH and O, and leads to their faster decay. The increase of N2 percentage also results in the increase and then decrease of the OH and O densities, but the change is smaller. Furthermore, when the H2O concentration is increased from 100 to 3000 ppm, the initial OH density increases slightly, but the OH density decays much faster, while the initial density of O decreases with the increase of the H2O concentration. After analysis, it is found that OH and O are mainly produced through electron collisional dissociation. O(1D) is critical for OH generation. O3 accelerates the consumption processes of OH and O at high O2 percentage. The addition of H2O in the NAPP considerably enhances the electronegativity, while it decreases the overall plasma reactivity, accelerates the decay of OH, and reduces the O atom density.

Development of thermally assisted OH PLIF temperature measurement method based on a single femtosecond laser

Recently, OH planar laser-induced fluorescence (PLIF) using the broadband, ultrashort femtosecond-duration (fs-duration) and the thermally assisted vibrational transfer in excited state has been investigated in flames. In this present work, we first measured temperature by thermally assisted OH laser-induced fluorescence (TALF) method with a single ultrashort broadband fs laser. In the experiment, the fs excitation of OH at ultraviolet wavelength is followed by fluorescence detection from two different vibrational bands. The ratio of two measured (1–0) and (0–0) band fluorescence is calibrated with calculated temperature using Chemkin PRO PRIMIX. The calibrated results are used in measuring temperature distributions in different laminar flames. It is found that TALF method using the fs laser can detect 2D temperature distribution in the burnt area with high OH fluorescence signal. However, OH chemiluminescence brings inevitable noise at the flame front that the TALF method does not perform well. And because (1–0) band fluorescence is so weak, the noise from the camera sensor and imaging intensifier (I.I.) remains at the measured temperature imaging. In conclusion, quantitative temperature measurement based on OH TALF based on a single broadband, ultrashort fs laser can be applied in laminar flames with high frequency by a simple experiment setup.

A Study on the Oil Film Thickness Between the Lower Rail of Oil Control Ring and Lower Flank of Oil Control Ring Groove of an Engine

Abstract Lubricating oil consumption (LOC) of engines causes particulate matter in exhaust gas and abnormal combustion like pre-ignition which becomes a serious problem in hydrogen engines. Lubricating oil transports upward into the combustion chamber of an engine via the sliding surface, the gap, and the side and back of a piston ring. The target of this study was to clarify the mechanism of oil flowing between an oil control ring lower flank and the groove which was the first entrance of lubricating oil supplied from the crankshaft. Oil film thickness at the lower flank of the oil control ring was measured by laser-induced fluorescence method using optical fibers embedded in the lower flank of the ring groove. The measured oil film thickness was compared with forces acting on the lower rail of three-piece type oil control ring. The oil film thickness change was well explained using those forces and it was found that friction force at the sliding surface and pressure in the ring groove were dominant on the oil film thickness under all operating conditions tested in this study.

Xenon tracers for cost effective laser induced fluorescence of alternative propellant Hall thrusters

One of the limiting factors to developing plasma thrusters on alternative propellants is the cost associated with changing the diagnostic tools, which are often propellant-dependent. For laser induced fluorescence (LIF), which is typically used for ion velocity distribution measurements to determine ion trajectories and potential profiles, either new lasers need to be bought, which are tuned to the wavelength of the new element’s excitation level, or a costly tunable laser is required. A method to use existing LIF setups designed for xenon on any propellant has been demonstrated on a Hall thruster operating on krypton. In the demonstration test, a small amount of xenon (0.01%–4%) was mixed with the main krypton propellant for use as a diagnostic tracer, and xenon ion velocities were measured while also monitoring changes in the mean discharge current and oscillations. High signal-to-noise ratios in LIF data acquired along the channel centerline were obtained with tracer gas fractions ≤1% that negligibly affected the thruster operation. These results and comparison of the emission spectra of xenon and other common propellants suggest that the tracer LIF method should be broadly applicable to LIF measurements in Hall thrusters operating on alternative propellants.

Investigation of the dielectric recovery process of vacuum arc in double breaks by planar laser-induced fluorescence

The multibreak vacuum circuit breaker uses multiple short gaps to interrupt the fault current, greatly improving the dielectric strength, and is a viable method to realize high-voltage interruption. The metal vapor distribution near the current zero is crucial for the dielectric recovery process in the multibreak vacuum circuit breaker. Due to the complicated dielectric construction and the interaction between the breakers, the vacuum arc inevitably deviates from the axisymmetric distribution during the interruption process. The traditional diagnosis method limited to 0D or 1D is not sufficient to study the real distribution of metal vapor near the current zero. To address these issues, we developed a planar laser-induced fluorescence method to measure the 2D distribution of copper vapor near the current zero by detecting 510.6 nm fluorescence intensity. The results indicate that for the butt contacts, the copper vapor is diffused in the gap of the high-voltage break and aggregated on the cathode surface of the low-voltage break. The axial magnetic field and transverse magnetic field affect the 2D copper vapor distribution and eliminate the inconsistency, which is achieved by affecting the motion of charged particles and the ionization-recombination process. Furthermore, the copper vapor density exhibits a positive dependence on the arc current, and the magnetic field impacts the density increase rate and distribution mode.

Three-dimensional flow velocity determination using laser-induced fluorescence method with asymmetric optical vortex beams

Laser-induced fluorescence (LIF) Doppler spectroscopy using an optical vortex beam with an asymmetric intensity distribution, referred to as aOVLIF, is proposed as a new method to measure plasma flow velocity. LIF spectra were calculated numerically using typical laboratory low-temperature plasma parameters, and it was revealed that an ion flow across the beam produces a frequency shift of the spectra. This method also has the capability of temperature measurements. The propagation effects of asymmetric optical vortex beams are discussed assuming an actual experiment, and it is found that the sensitivity to the transverse flow velocity is approximately unchanged. The aOVLIF method, which exploits the inhomogeneous phase structure of optical vortices, can be applied to the determination of three-dimensional velocity vectors and promises to enhance the usefulness of conventional LIF spectroscopy using plane waves.

Pixel-dependent laser-induced fluorescence method for determining thin liquid film thickness distribution

Thin liquid films on free surfaces or inside restricted spaces are widely encountered and can have significant effects in heat transfer, coating, biofilm growth, and lubrication. The thickness and distribution of these thin liquid films are important determinants of performance, but cannot easily be measured accurately. This paper presents a methodological study on determining the thickness of thin liquid films by means of laser-induced fluorescence. A model of fluorescence imaging and measurement is established based on the fluorescence energy transmission. A novel pixel-dependent laser-induced fluorescence method is then proposed and compared with commonly used methods using experimental test results. The accuracy and precision of the measurements are discussed in detail. It is found that the measurement performance is greatly influenced by the nonlinearity, heterogeneity, and random noise of the optical system and imaging technique. The proposed pixel-dependent laser-induced fluorescence method effectively eliminates or ameliorates the effects of these factors and enhances the measuring accuracy.

Identification of marine microplastics based on laser-induced fluorescence and principal component analysis

Although the laser-induced fluorescence method shows great potential for microplastic particle detection, overlapping fluorescence signals make accurate type and proportion identification difficult. This paper presents the identification of marine microplastics based on laser-induced fluorescence and principal component analysis. This method works by measuring the fluorescence spectra of water-containing microplastic samples irradiated with a 405-nm laser, which are then analyzed using the principal component analysis (PCA) method. The nine types of microplastics were differentiated based on their positions in the PCA score plot. The mixed sample was positioned between the pure microplastic samples. The component ratio determines its position relative to that of the pure microplastic samples. The first two principal components of the mixed microplastics were linearly dependent. Natural seawater had less influence on the detection, and a mass concentration as low as 0.03% was detected.

Impact of pilot flame and hydrogen enrichment on turbulent methane/hydrogen/air swirling premixed flames in a model gas turbine combustor

This work investigates the impact of pilot flame and fuel composition on the structures and stabilization of swirling turbulent premixed methane/hydrogen/air flames in a lab-scale gas turbine model combustor. Simultaneous measurements of the velocity field and OH radicals distribution in the combustor were conducted using particle imaging velocimetry (PIV) and planar laser-induced fluorescence (PLIF) methods, respectively. Flames under stable and close to lean blow-off (LBO) conditions were studied for two fuel mixtures, with a hydrogen mole ratio of 0 and 50 % in the hydrogen/methane mixture, respectively. The studied flames were at a constant Reynolds number of 20,000 with different equivalence ratios. Two pilot-to-global fuel ratios were investigated (2 % and 6 %) while keeping the pilot-to-global air ratio constant at 2 %. Data for non-piloted flames were also acquired for comparison. The pilot flames were shown to extend the operability range. The LBO equivalence ratio of the main flame decreased with increasing fuel mass flow rate in the pilot flames due to the increased amount of hot gases with high concentrations of OH radicals in the outer recirculation zone (ORZ), which significantly enhanced the stabilization of the main flame. The stable flame reaction zone was in the high-speed shear layer between the ORZ and the inner recirculation zone (IRZ). When approaching LBO, the reaction zone was pushed downstream to the IRZ and subsequently decreased the size of IRZ, indicating a strong flow/flame interaction. Hydrogen enrichment was shown to reduce the LBO equivalence ratio. When close to LBO, the OH radicals in the hydrogen-enriched flames were observed in isolated pockets due to differential diffusion, which enhanced resilience to LBO. The flame front curvature, mean progress variable, and flame surface density were calculated from the acquired OH-PLIF data to quantify the impact of fuel composition and pilot flames on the flame structures.

‘Self-calibration’ method for TALIF measurement of O atoms by full photofragmentation of O3 using a 226 nm UV laser

Quantification of atomic oxygen through the method of two-photon absorption laser-induced fluorescence (TALIF) is common in the fields of plasma fundamental research and application treatments. Fluorescence signal calibration is required to absolutely quantify the O amount and normally achieved with the help of TALIF measurement of a known-density Xe gas. In this study, an alternative calibration method is proposed based on the full photofragmentation (FPF) of O3 with a known density in a known gas composition by the same UV laser beam as that of the TALIF detection of atomic O. This is achieved by an equivalent amount of O fragment contributing the same fluorescence intensity as that of the O3 FPF-TALIF process under the same experimental conditions. The validity of this calibration method is proved by comparing it to the TALIF measurement of the Xe gas. It provides a ‘self-calibration’ method for the TALIF detection of O atoms without any need to change the laser optical arrangements including the laser wavelength. In addition, it only requires a gas flow with known O3 density through the studied medium reactor or chamber (such as plasma discharges). Detailed theoretical and practical principles of this self-calibration approach are presented and discussed in this study.