Test Method Research Articles

Cranioplasty infection in porous hydroxyapatite: Potential antibacterial properties.

Intensive research is dedicated to the development of novel biomaterials and medical devices to be used as grafts in reconstructive surgery, with the purpose of enhancing their therapeutic effectiveness, safety, and durability. A variety of biomaterials, from autologous bone to polymethylmetacrylate, polyether ether ketone, titanium, and calcium-based ceramics are used in cranioplasty. Porous hydroxyapatite (PHA) is reported as a possible material for bone reconstruction, with good signs of biocompatibility, osteoconductive and osteointegrative properties. In the present paper we studied the possible antibacterial properties of PHA in a laboratory test in order to provide a possible overview of the occurrence of post-operative infections in PHA cranioplasty. The test method has been designed to evaluate the potential antimicrobial activity of specimens under dynamic contact conditions to overcome difficulties in ensuring contact of inoculum to the specimen surface. The test was conducted using Staphylococcus aureus ATCC6538 as a bacterial strain. Two experimental sets were performed to evaluate the antimicrobial properties of the specimens against two different Staphylococcus aureus concentrations. The first preliminary test (a) verified the antibacterial property at 0, 1, 2, and 4 h of contact time; the second confirmatory test (b) was repeated to verify the antibacterial property at 0, 4, 8 h. In the first experiment, after the first hour of contact, the bacterial inoculum was reduced by 7.96% compared to "inoculum only," which increased to 26.11% at the second hour, and up to 52.33% after 4 h. In the second experiment, the confirmation test showed that bacterial growth reaches maximum inhibition after 4 h of contact. At 4 h, there was a higher bacterial reduction of 72.93%, which decreased at 8 h (36.45%). Analyzing the growth trend of viable microorganisms under Dynamic Contact Conditions it can be seen that PHA cranioplasty appears to inhibit exponential growth by inducing bacterial stasis in the early hours of contact, reaching a maximum reduction within 4 h, in this adopted experimental condition.

Unique Research for Developing a Full Factorial Design Evaluated Liquid Chromatography Technique for Estimating Budesonide and Formoterol Fumarate Dihydrate in the Presence of Specified and Degradation Impurities in Dry Powder Inhalation.

A simple LC method has been developed and validated for estimating budesonide (epimer B + A) and formoterol fumarate dihydrate in dry powder inhalation. The development results of this study make it very significant. The degradation and process impurities in EP and ChP were identified in addition to budesonide and formoterol fumarate. As of yet, no one has reported all impurities using a single method. It is a unique research because it analyzes APSD (Aerodynamic Particle Size Distribution), DDU (Delivered Dose Uniformity), BU (Blend Uniformity), Assay, and cleaning test samples. It enhances the quality of medicine and separates all organic impurities and isomers through a suitable stationary phase (YMC-Pack Pro C18, 150 × 4.6 mm × 3 μm). We optimized the chromatographic conditions: Injection volume was 20 μL, and flow rate was 1.0 mL/min. The wavelength was optimized at 220 nm. After experimental and validation results. An example is A, which contains sodium dihydrogen orthophosphate monohydrate, sodium 1-decane sulfonate, adjusted pH 3.0, and acetonitrile at a ratio of 80:20 (v/v), and B, which contains pH 3.0 buffer and acetonitrile at a ratio of 20:80 (v/v) respectively. In addition to being optimized, the test method was validated according to ICH Q2(R2).

Impact of HWD dynamic loading on measured deflection basins and back-calculated stiffnesses of flexible airport pavements

Accurate measurement of pavement structural response is critical when evaluating the condition and load-bearing capacity of a flexible airport pavement. Pavement surface deflection basins tested by Heavy Weight Deflectometer (HWD) have been widely applied as a non-destructive testing method to analyse the elastic modulus of pavement layers via a back-calculation procedure. A clear linear relationship between measured deflections and HWD drop loads was established, with R2>0.99. The studied flexible pavement structure showed linear elastic responses under different drop loads, with relatively stable elastic moduli of pavement layers. With the increasing drop load (DL), the back-calculated hot-mix asphalt (HMA) surface modulus was slightly influenced. The results also identified the existing buried sensors may affect the measured HWD responses. By analysing statistical results, this study provides a benchmark for airport owners to evaluate the structural conditions for their flexible pavements when applying HWD in their maintenance and rehabilitation (M&R) works.

Comparison of MIC Test Strip and reference broth microdilution method for amphotericin B and azoles susceptibility testing on wild type and non-wild type Aspergillus species.

This study was performed to evaluate whether the MIC Test Strip (MTS) quantitative assay for determining the minimum inhibitory concentration (MIC) correlated with the CLSI reference broth microdilution method (BMD) for antifungal susceptibility testing of wild-type and non-wild-type Aspergillus species isolated from cystic fibrosis patients against antifungal agents known to be usually effective against Aspergillus spp. This study was performed to assist in the decision-making process for possible deployment of the MTS assay for antimicrobial susceptibility testing of Aspergillus species into regional public health laboratories of Mycology due to difficulties in equipping the reference BMD methods in a laboratory routine. For this purpose, a set of 40 phenotypically diverse isolates (27 wild-type, 9 non-wild-type, and 4 species with reduced susceptibility to azoles and amphotericin B (AMB)) collected from clinical samples were tested. MICs were performed by both MTS and reference BMD for AMB, and azoles. MTS results for posaconazole correlated well with reference BMD rendering an almost perfect agreement (kappa value=1.000) by category interpretation (CI)/category distribution of MICs (CDM) (100%) while voriconazole MTS results yielded a substantial correlation with BMD (kappa value=0.788) by CI/CDM (97.5%). In contrast, itraconazole and AMB yielded the poorest correlation with BMD, rendering a moderate agreement (kappa values of 0.554 and 0.437, respectively) by CI/CDM (87.5% and 85%, respectively). In conclusion, the MTS method represents a valid option for antimicrobial susceptibility testing of Aspergillus species against posaconazole and voriconazole. Itraconazole and AMB MTS results showed some concerning lack of correlation with the corresponding reference BMD results.

Evaluating the impacts of large-scale opencast copper mining on vegetation productivity change in recent thirty years: a case study of Dexing Copper Mine

Understanding the impact of mining on surrounding vegetation gross primary productivity (GPP) is crucial for the ecological management of mining areas. Using the simple non-iterative clustering (SNIC) and random forest (RF) methods, we mapped the Dexing Copper Mine (DCM) at a 30 m spatial resolution with Landsat data from 1991 to 2022. The vegetation photosynthesis model (VPM) was selected to simulate vegetation GPP. Sens slope test and Hurst exponent method jointly used to reveal the spatial-temporal evolution of vegetation GPP. Results showed: (1) SNIC+RF was more effective than RF alone. The mine area expanded from 5.23 km2 to 24.97 km2 due to deforestation; (2) The vegetation GPP in 53.17% of the areas has significantly increased. The GPP reduction areas are primarily around the open pit and the tailings; (3) Vegetation GPP loss due to land cover change was −2×1010 gC, −2.7×1010 gC, and −2.79×1010 gC for 1991-2000, 2000-2012, and 2012-2022.

Wafer-scale mapping of carrier density and mobility with terahertz time-domain ellipsometry

Terahertz (THz) measurements are increasingly valued for nondestructive testing of materials in power devices and other applications. Hence, there is a growing demand for highly accurate characterization methods in the THz range. Here we demonstrate the application of THz time-domain ellipsometry (THz-TDE) to large-scale, quantitative mapping of semiconductor wafers. While THz-TDE is an established technique, its application in wafer-scale mapping, which is an important process in the semiconductor industry, has not yet been demonstrated. In this work, we highlight the effectiveness of THz-TDE by mapping the electrical properties of a widely used semiconductor, silicon carbide (SiC). Spatial distribution maps of conductivity, carrier density, and mobility of a commercial 4-in. SiC wafer are derived using the measured ellipsometric parameters. THz-TDE mapping offers a nondestructive, contactless testing method to evaluate semiconductor quality and electrical homogeneity and is notably suitable for doped semiconductors characterized by high THz absorption.

Analysis of The Influence of Visible Safety Leadership and Job Satisfaction on Safety Work Performance at PT. Solusi Bangun Indonesia Tbk Pabrik Cilacap

This research aims to analyze the influence of visible safety leadership and job satisfaction on safety work performance at PT. Solusi Bangun Indonesia Tbk Cilacap Factory. This research uses quantitative research methods with descriptive and analytical approaches. The research subjects were employees and contractors of PT. Solusi Bangun Indonesia Tbk Cilacap Factory involved in overhaul activities. Data collection was carried out randomly through questionnaires, interviews and observations. Research data was analyzed using quantitative statistical test techniques and then described in detail based on the results of each test. The research results show that work accidents at PT. Solusi Bangun Indonesia decreased during the 2023 overhaul activities due to its leadership role in ensuring workplace safety during these activities. This research highlights how visible leadership behavior in the field, or visible safety leadership and job satisfaction, contributes to increasing safety work performance. The Regression and Correlation Test Method used to analyze the data shows that 76.4% of safety work performance is influenced by visible safety leadership and job satisfaction, while the rest is influenced 23,6 % by other factors. These results confirm that leadership behavior that is proactively involved and visible in the field and high job satisfaction in implementing safety has a significant impact on safety performance, which ultimately contributes to a safer work environment for all workers.

A comparison of results of determining the hydrocarbon group-type composition of petroleum samples obtained using various test methods of open-column liquid chromatography

The hydrocarbon group-type composition (so-called SARA-composition) is one of the main characteristics of oil, providing an information about its chemical nature and determining the quality of the obtained petroleum products. In world laboratory practice, data on the hydrocarbon group-type composition of petroleum feedstock are widely used to assess its colloidal stability and compatibility with crude oil, reactivity in various conversion processes, predicting physical properties, etc. Traditionally, the hydrocarbon group-type composition is determined in accordance with standard and research chromatographic methods that differ significantly from each other, which often leads to obtaining incomparable analytical results. In this paper, a comparative analysis of the results of determining the hydrocarbon group-type composition obtained in accordance with two test methods within the open-column liquid chromatography method is carried out for various of petroleum samples of Russian origin (commercial oils of different classes and two petroleum products). Using the standard test method ASTM D4124 (method B) and the research test method developed by JSC VNII NP, the content of identical hydrocarbon groups in the maltene part of petroleum samples was determined: saturated hydrocarbons (SH) and paraffinic-naphthenic hydrocarbons (PNH), naphthenic aromatic (NAH) and aromatic hydrocarbons (AH), polar aromatic hydrocarbons (PAH) and resins (R), respectively. It is shown that with an increase the density and viscosity of petroleum samples, the content of less polar groups (SH and PNH) in their composition decreases and the content of more polar groups (PAH and R) obviously increases, and there is no pronounced distribution pattern of the NAH and AH groups. Statistical processing revealed insignificant differences in the results for «heavy and viscous» oils, despite the large error of those obtained according to the research test method. Obtaining comparable results justifies the use of both test methods not only for the analysis of high-boiling petroleum products, but also for the analysis «heavy and viscous» oil samples.

Design and Testing of a Peanut Roller Digging Harvester

In order to solve the problems that arise from poor fruit–soil separation, a high fruit burial rate, and a high crushing rate during peanut harvesting under clay-heavy soil conditions, a multistage roller peanut harvester that is suitable for operation in these environments was designed. Considering the growth conditions of peanuts and the agronomic requirements of planting, the movement process and separation operation mechanism of the seedling and fruit–soil were analyzed, coupled with the vibrating excavation device and the conveying roller group. A dynamic model of seedling fruit–soil coupled throwing and soil collisions on the conveyor roller was constructed. The structure and maximum swing angle of the vibrating excavation device, the number of stages of the conveying roller group, the diameter of the throwing roller, and other parameters were determined through analyses. Utilizing the design results, a prototype was built, and a multifactor test for the operation parameters was carried out. The forward speed of the machine, the excavation depth, and the speed of the conveyor roller were used as the test factors. The fruit burial rate and the peanut crushing rate were used as the test indexes. The Box–Behnken test method was used to establish a regression equation between the factors and the test indexes, and the influence law of each factor on the peanut harvest index was determined. When the optimal parameters were combined, i.e., 1.0 m/s, 150 mm, and 80 r/min, the fruit burial rate and crushing rate of peanuts were 6.2% and 6.4%, respectively. The performance of the machine meets the design requirements, and our research results can provide a reference for the research of mechanized peanut harvesting technology and equipment.

Identification of Key Risk Factors in Mechanical Cross Passage Construction Based on the Decision-Making Test and Evaluation Test Method and the Interpretation Structure Model

In order to solve the problem that the key risk factors in the construction of mechanical cross passages are relatively vague, the decision-making test and evaluation test method (DEMATEL) and the interpretation structure model (ISM) are combined to analyze safety risks in the construction of mechanical cross passages scientifically and reasonably. Based on the ‘personnel’, ‘material’, ‘machine’, ‘method’, and ‘environment’ of 4M1E comprehensive management, the construction safety risk index system of mechanical method cross passage, including 18 risk factors, is extracted. DEMATEL was used to divide the risk factors into four factor sets: strong cause, weak cause, strong result, and weak result. Furthermore, ISM is used to construct a hierarchical structure diagram of risk factors, and deep risk factors and risk factors with a high node degree are divided. The results show that the safety awareness of construction personnel, the safety technology level of construction personnel, the establishment and implementation of the safety construction system, the level of construction management, and the degree of geological complexity are strong cause-type risk factors with both high centrality and high causality. Additionally, the effect of reinforcement of the internal support system, the setting of the digging parameter, the effect of grouting, and the effect of assembling the pipe sheet comprise the strong result-type risk factors with high centrality. The risk factors with higher node degree in ISM are consistent with the risk factors with higher centrality in the DEMATEL model, which are key factors that play an important role in risk control.

Self-sampling and self-testing for HIV at a commercial and community-based test provider in the Netherlands: user preferences and usability

HIV self-sampling and -testing (HIVSS/ST) reduces testing barriers and potentially reaches populations who may not test otherwise. In the Netherlands, at-home HIV tests became commercially available around 2016, but data on user experiences are limited. This study aimed to explore characteristics of users and their experiences with HIVSS/ST. In 2022 and 2023, a survey was distributed among users of one online commercial provider and one community-based provider; either users ordered a commercial online HIVSS/ST or they sought a free-of-charge HIVST via the community-based provider. Questions included usability, preferences, and barriers of HIVSS/ST-testing. We compared characteristics and experiences of users between providers, risk groups and first-time and repeat testers. In total, 133 users completed the survey; 89 (67%) via the commercial provider and 44 (33%) via the community-based provider. Response rates per provider were 2% and 26%, respectively. Users who bought their test at the commercial provider were more often Gay and Bisexual Men (GBM) (42%), 35 + years (43%), and born in the Netherlands (89%), compared to those recruited through the community-provider (respectively 27%, 25%, 14%). GBM were more often repeat and recent HIVSS/ST-testers, and using pre-exposure prophylaxis (PrEP). Women and heterosexual men were more likely to buy an HIVSS/ST as part of a combination Sexually Transmitted Infections (STI) test package. Overall, main reasons for choosing HIVSS/ST were saving time (42%), anonymity (36%) and not having to talk to a GP (35%). Twenty-two percent of the study participants experienced some problems performing the HIVSS/ST, the most reported problem was obtaining sufficient blood through the finger prick (71%). Recommendations to improve accessibility of HIVSS/ST included more awareness (advertising by trustful providers), more access locations (pharmacies/supermarkets/schools) and lower costs. Our findings indicate that HIVSS/ST is a valuable additional HIV testing method for users, but more insight into the contribution of HIVSS/ST to HIV prevention policies is needed.

Assessment of silica sand behavior around rotating square rod in cylindrical container via ultrasonic velocity profiling

Abstract This study aims to establish a test method for simultaneously evaluating material erosion and corrosion, and rotating-material wear tests were conducted using a slurry pot. The erosion and corrosion scars of specimens were assessed from photographic images, and wear condition diagrams organized by dimensionless particle size (d p /d) and Reynolds number (Re) were constructed. Furthermore, the flow structure and particle behaviour around each specimen were visualized via ultrasonic velocity profiling to confirm the mechanism of the ratio change between erosion and corrosion. Results showed that a vortex flow formed around the specimen near the bottom of the container, which caused erosion. The outward flow height due to the vortex varied at Reynolds numbers of 5 × 103 ≤ Re ≤ 1 × 104 and sand particle concentrations of 2.24 ≤ C v ≤ 40 vol%. It decreased with increasing Re and increased with C v , which was consistent with the trend in the surface wear images. The proposed wear condition diagram was supported by flow visualization, which showed that erosion- and corrosion-dominated regions could be distinguished from the amount of remaining rust. Graphical abstract

Time to dietary diversity of complementary feeding improvements and its associated factors among infants aged 6–12 months in Ethiopia: evidence from performance monitoring for action

BackgroundInfant and Young Child Feeding significantly affect the health, development, and nutritional status of children under 2 years old, ultimately affecting their survival. The aim of this study is to determine the time to improvement in dietary diversity and associated factors in infants aged 6 to 12 months.MethodsThe study used secondary data from the PMA Ethiopia longitudinal panel survey, involving pregnant women from January to March 2024. The data management and analysis were performed using Stata version 17. The Kaplan–Meier survival curve (KM) and the log-rank test method were implemented. A Cox proportional-hazard regression model was used to explore the association between independent variables and the outcome variable. The strength of the association was indicated by the adjusted hazard ratio (AHR) with a 95% confidence interval. The threshold of p < 0.05 was applied to determine the significance of an association.ResultsThe study found that the proportion of infants with improved dietary diversity aged 6–12 months was 22% (95% CI: 19.5, 25%). Factors associated with improved dietary diversity in infants aged 6 to 12 months were married women (AHR = 9.3, 95% CI = 1.19, 8.30), women with a secondary school (AHR = 1.9, 95% CI = 1.05, 3.51), women with technical and vocational (AHR = 2.0, 95% CI = 1.01, 4.05) and women with a university degree (AHR = 2.9, 95% CI = 1.51, 5.38). Moreover, women in the highest wealth quintile (AHR = 3.5, 95% CI = 1.31, 9.41), women visiting PNC (AHR = 1.7, 95% CI = 1.13, 2.62), women visiting ANC 1–3, and more than four times were (AHR = 2.4, 95% CI = 1.51, 3.74) and (AHR = 3.6, 95% CI = 2.28, 5.67) times higher for improving dietary diversity.ConclusionThe findings of this study showed that the proportion of dietary diversity improvement was 22%. Which is relatively low. Marital status, educational status, wealth index, PNC, and ANC visits were identified as statistically significant factors associated with dietary diversity improvements. It suggests that public health interventions should focus on enhancing maternal knowledge and promoting regular healthcare visits to mitigate malnutrition and improve infant health outcomes in Ethiopia.

A Evaluation of Chatbot Responsiveness and Accuracy Using a Matching Pattern Algorithm for Implementation in a Medium-Sized Enterprise

This study aims to develop a website-based chatbot system using a matching pattern algorithm to improve the efficiency of the store department at PT.XYZ in managing warehouse inventory. The system is designed to assist users in adding item names, images, and quantities to the database in a more structured and efficient manner. The system development process was carried out using the SDLC method to ensure a systematic approach, while the performance of the developed features was tested using the black box testing method. The results of this study indicate that the web-based chatbot successfully displays item information based on user requests. Additionally, the system provides an option to add new items to the database if the requested item is not found, automatically updating the knowledge base with relevant string patterns. Black box testing results demonstrate that all key features, such as item search, quantity adjustment, and image display, function reliably and effectively. Thus, this chatbot system has proven to significantly support the operational efficiency of the store department at PT.XYZ.

Determination Method of Optimal Decomposition Level of Discrete Wavelet Based on Joint Jarque–Bera Test and Combination Weighting Method

Determination Method of Optimal Decomposition Level of Discrete Wavelet Based on Joint Jarque–Bera Test and Combination Weighting Method

Research on the Influence Mechanism of Environment Social Government Performance in State-Owned Enterprise Value: The Role of Digital Transformation

Under the background of sustainable development and high-quality development in China, it is of great significance to actively promote enterprises to practice ESG development concepts and digital transformation. This study uses a sample of state-owned companies in the Chinese capital market during the period from 2014 to 2020. The results of the research show that there is a U-shaped relationship between ESG performance and the value of state-owned enterprises. And digital transformation plays a positive moderating role in the relationship. The result of a further mechanism study indicates that ESG performance affects enterprise value by reducing the financing constraints. This study also examines the results using a dynamic panel model and endogeneity test method. Therefore, this study provides references to promote ESG practices and offers new insight into the role of digital transformation in state-owned companies. In order to pursue sustainable development and high-quality economic development, ESG practices and digital transformation should also be paid attention to in enterprises.

Bond Performance of GFRP Bars in Glass and Basalt Fiber-Reinforced Geopolymer Concrete Under Hinged Beam Tests

In recent years, researchers have focused on the usability of fiber-reinforced polymer (FRP) bars due to their lightweight, corrosion-resistant, and eco-friendly characteristics. Geopolymers, as low-carbon alternatives to traditional binders, aim to reduce CO2 emissions in concrete production. The bond strength between FRP bars and concrete is critical for the load-bearing capacity and deformation characteristics of reinforced elements. The objectives of this work are to investigate the bond performance of GFRP bars in chopped glass and basalt fiber-added geopolymer concrete using hinged beam tests. Since the hinged beam test accurately represents the behavior of real bending elements, this test method was selected as a main bonding test. Initially, three geopolymer mixtures with Ms modulus values of 1.2, 1.3, and 1.4 were prepared and tested. The mixture with a modulus of 1.2 Ms, achieving a compressive strength of 56.53 MPa, a flexural strength of 3.54 MPa, and a flow diameter of 57 cm, was chosen for beam production due to its optimal workability and strength. After mechanical and workability tests, SEM analysis was performed to evaluate its internal structure. For evaluating the bond performance of GFRP bars, 12 geopolymer beam specimens were prepared, incorporating varying fiber types (chopped glass fiber or basalt fiber) and embedment lengths (5 Ø or 20 Ø). Hinged beam tests revealed that the bond strengths of glass and basalt fiber-added mixtures were up to 49% and 37% higher than that of the control geopolymer concrete, respectively. It was concluded that incorporating fibers positively influenced the bond between geopolymer concrete and GFRP bars, with glass fibers proving more effective than basalt fibers. These findings enhance the understanding of bond mechanisms between GFRP bars and geopolymer concrete, emphasizing their potential for sustainable and durable construction in both industrial and scientific applications.

A Comparison of the Next Eigenvalue Sufficiency Test to Other Stopping Rules for the Number of Factors in Factor Analysis.

A plethora of techniques exist to determine the number of factors to retain in exploratory factor analysis. A recent and promising technique is the Next Eigenvalue Sufficiency Test (NEST), but has not been systematically compared with well-established stopping rules. The present study proposes a simulation with synthetic factor structures to compare NEST, parallel analysis, sequential test, Hull method, and the empirical Kaiser criterion. The structures were based on 24 variables containing one to eight factors, loadings ranged from .40 to .80, inter-factor correlations ranged from .00 to .30, and three sample sizes were used. In total, 360 scenarios were replicated 1,000 times. Performance was evaluated in terms of accuracy (correct identification of dimensionality) and bias (tendency to over- or underestimate dimensionality). Overall, NEST showed the best overall performances, especially in hard conditions where it had to detect small but meaningful factors. It had a tendency to underextract, but to a lesser extent than other methods. The second best method was parallel analysis by being more liberal in harder cases. The three other stopping rules had pitfalls: sequential test and Hull method even in some easy conditions; the empirical Kaiser criterion in hard conditions.

Modified field method for testing activated sludge condition in chambers with variable wastewater levels.

The paper presents a proposal to modify a field method of testing the condition of activated sludge using a 30-min volume of sludge (settling test). To verify the validity of the modified method of testing the condition of activated sludge, field tests were performed in two onsite wastewater treatment plants. In these plants, the reaction chambers were fed by gravity from the primary sedimentation tank throughout the day. For such treatment plants, the activated sludge condition test varies during a 24-h period. Testing the condition of activated sludge using the modified volume of sludge gives stable results over a 24-h period. To analyse the results, a statistical test was performed to compare the results obtained in the traditional volume of sludge and the modified one. The analysis showed that the results of the sludge volume test are incorrect if the wastewater sample is taken at a time other than the final filling of the bioreactor (just before the sedimentation phase) due to sludge thickening. The proposed modified sludge volume test allows the operator to perform the test at any time of day with a correct result.

Retrospective Evaluation of Toxoplasma gondii Test Results in Patients Applying to A Research Hospital.

This study retrospectively evaluates our laboratory Toxoplasma gondii immunoglobulin (Ig)M, IgG antibody and avidity test results to determine the distribution of the pathogen according to sex, age, clinics and years. The serum samples sent to Pamukkale University Healthcare Research and Practice Hospital's Medical Microbiology laboratory between January 2016 and December 2023 were evaluated for T. gondii seropositivity. Toxoplasma antibodies and avidity testing were studied using chemiluminescent microparticle immunological testing method (Abbott Architect i2000SR, Weisbaden, Germany). The study evaluated 42,817 tests ordered from patients with suspected toxoplasmosis. Five hundred-three (2.1%) were found to be anti-T. gondii-IgM positive and 4.406 (24.7%) to be anti-T. gondii-IgG positive. Anti-T. gondii-IgM and anti-T. gondii-IgG positivity were significantly higher in women than in men (p=0.0001; p=0.0001, respectively). Anti-T. gondii-IgM positivity was found to be high between the ages of 19 and 49 (p=0.0001). The anti-T. gondii-IgG positivity rate increased with age (p=0.0001). Anti-T. gondii-IgM and anti-T. gondii-IgG positivity rates have decreased significantly over the last four years (2020-2023) compared to the first four years (2016-2019) (p=0.0001; p=0.0001, respectively). When the results of 582 patients with T. gondii-avidity requests were evaluated, 38.1% of the anti-T. gondii-IgM positive patients and 7.8% of the anti-T. gondii-IgM negative patients had low avidity. T. gondii seroprevalence was found to be similar to the global average, with female sex, age and nationality among the risk factors. It has also been observed that seroprevalence has significantly decreased in recent years.