Design Method Research Articles

GGAssembler: Precise and economical design and synthesis of combinatorial mutation libraries.

Golden Gate assembly (GGA) can seamlessly generate full-length genes from DNA fragments. In principle, GGA could be used to design combinatorial mutation libraries for protein engineering, but creating accurate, complex, and cost-effective libraries has been challenging. We present GGAssembler, a graph-theoretical method for economical design of DNA fragments that assemble a combinatorial library that encodes any desired diversity. We used GGAssembler for one-pot in vitro assembly of camelid antibody libraries comprising >105 variants with DNA costs <0.007$ per variant and dropping significantly with increased library complexity. >93% of the desired variants were present in the assembly product and >99% were represented within the expected order of magnitude as verified by deep sequencing. The GGAssembler workflow is, therefore, an accurate approach for generating complex variant libraries that may drastically reduce costs and accelerate discovery and optimization of antibodies, enzymes and other proteins. The workflow is accessible through a Google Colab notebook at https://github.com/Fleishman-Lab/GGAssembler.

Optimized Design Method Based on Parallel Stepwise Hierarchical Constraints and its Application for 1-DOF Six-Bar Finger

Optimized Design Method Based on Parallel Stepwise Hierarchical Constraints and its Application for 1-DOF Six-Bar Finger

A D-T Hybrid DC FCL and Its Parameter Optimization Design Method for Application in Multiterminal VSC-HVDC Grids

A D-T Hybrid DC FCL and Its Parameter Optimization Design Method for Application in Multiterminal VSC-HVDC Grids

Co-producing and co-assessing a new service solution for enhancing health and social care integration: a participatory research protocol

BackgroundThis paper describes a study protocol for co-producing and co-assessing a new sustainable and scalable service solution that enhances health and social integration by involving providers and volunteers delivering services for elderly people in the province of Cremona (Italy), where the elderly population will reach 27% in 2023.MethodsThis upcoming study involves mixed-method participatory research and is structured in three study phases and related objectives. First, it will co-produce a new, accessible and sustainable service solution using an iterative design and management method, Plan-Do-Check-Act by involving professionals and volunteers of a heterogeneous group of health, social and third sector organizations located in the city of Cremona (Italy). Second, the study protocol will co-assess the outcomes of the new service solution using a mixed-method approach for measuring the outcomes on: professionals and volunteers (micro level) and their health, social and third sector organizations (meso level). Third, this study will co-investigate the scalability of the new solution promoting health and social integration in other similar urban areas of the Province of Cremona via the Intervention Scalability Assessment Tool (macro level). The data will be collected through the analysis of official documents, websites, policies and participatory workshops.DiscussionThis protocol proposes an innovative intervention, a novel participatory approach, and an unexplored scalability assessment tool in the context of health and social care integration. This study aims to support professionals from health and social care service providers and volunteers from third-sector organizations to collaborate and integrate each other’s resources. In doing so, the participatory approach will facilitate the co-creation of an effective response to the need of health and social integration, and the development of trustful relationships between health and social care service providers. Moreover, the adoption of Plan-Do-Check-Act and Intervention Scalability Assessment Tool will ensure the quality, scalability and sustainability of the new service solution in other settings.

Fast inverse design of microwave and infrared Bi-stealth metamaterials based on equivalent circuit model

This work proposed a fast inverse design method for microwave and infrared (IR) bi-stealth metamaterials based on the equivalent circuit model (ECM). Using this method, we designed a microwave and IR bi-stealth metamaterial by deploying a multilayered structure of the indium tin oxide (ITO) film based metasurface. First, the IR emissivity of the ITO film was calculated in the framework of the ECM. Then, an ITO metasurface was proposed to implement low IR emission and high microwave transmission simultaneously. Based on the ECM of the square patch, the ECM of the whole metamaterial was established at the microwave band. An inverse design program was built by incorporating the ECM with genetic algorithm (GA). Structure parameters of the metamaterial were optimized by GA to achieve the broadest microwave stealth bandwidth for the given thickness. Finally, the sample of the optimized bi-stealth metamaterial was prepared and tested. The calculated, simulated, and measured results are in good agreement, showing that such a metamaterial has an IR emissivity of 0.18 in the band from 3 to 14 μm and an efficient microwave stealth band from 4.8 to 17 GHz with a thickness of 4.9 mm. The proposed method will benefit the design and application of microwave and IR bi-stealth metamaterials.

An optimum structural design method for a torque sensor measuring the control moment of the micro flapping-wing robot

Abstract This paper presents an optimum structural design method for a sensor capable of measuring the control torque of the micro flapping-wing robot. Torque measurement in flapping-wing robots has special demands on the sensor bandwidth. The method in this paper focuses on the development of a multi-objective optimization method based on the surrogate model to solve the sensor indicator design issues, for example, increasing sensitivity while meeting bandwidth requirements. Initially, Latin hypercube sampling was applied to the choice of the characteristic parameters of the sensor. Based on finite element techniques, the surrogate models describing displacement and eigenfrequency were established to characterize the sensor indicators, and a multi-objective optimization was conducted. According to the optimization results, the sensor structure was manufactured, and a torque measurement platform was set up. Calibration experiments and frequency response experiments demonstrated a high level of consistency between the surrogate model and the experimental data. With the assistance of the eddy current displacement sensor, the actual displacement sensitivity coefficient of the torque sensor is determined to be 0.4325 mm mNm−1, consistent with the calculation of the surrogate model. The characteristic frequency is 488.5 Hz, with a relative error of 7.47% compared to the surrogate model. This indicates that the optimum structural design method can be utilized for the rapid design of torque sensors for special requirements, thus laying the foundation for the control torque measurement of micro flapping-wing robots.

Potential Utilization of Defective Fruit Juice Products for Black Soldier Fly Cultivation to Reduce Waste Generation in the Beverage Industry

The waste of fruit juice and tea defective products has organic content that conventionally has the potential to be utilized as a medium or place for maggot growth, but until now it has not been equipped with supporting scientific studies. Therefore, the objectives of the study are: 1) Assessing the characteristics of fruit juice and tea product waste, 2) Assessing the effectiveness of maggot in reducing fruit juice and tea product waste. This research is experimental in nature using variations in the composition of fruit juice and tea product waste as a source of nutrition for maggot which is carried out for 12 days. This variation uses the Complete Randomized Design method with 3 (three) treatments, namely variations in the composition of 60% tea + 40% fruit juice waste (S1), 50% tea + 50% fruit juice waste (S2), and 40% tea + 60% fruit juice waste (S3) in duplo. In addition, this study was completed with a control treatment. The results showed that the characteristics of fruit juice and tea waste containing protein (3.78-4.59%), fat (0.90-1.31%), carbohydrates (28.15-29.15%), crude fiber (2.42-3.12%) can be used as maggot cultivation media. The highest protein and crude fiber contents were found in the S3 composition waste at 4.59% and 3.12%. Maggot cultivation results that showed the highest fat and carbohydrate content were found in S1 composition waste at 1.31% and 29.15%. The recommended composition variation is S1 composition with the highest substrate consumption value and waste reduction index of 57.47% and 20.06%.

Comparison of copper-based composites fabricated by cross-accumulative roll bonding method for packaging design

Copper-based composites have interesting properties and they are widely used in electronics, packaging, and automotive applications. In the present work, the cross-accumulative roll bonding (CARB) process was used to fabricate Cu-based composites with Zn and Brass reinforcing layers. The experimental observations revealed a clear relationship between rolling strain and plastic instabilities including the necking and rupture of reinforcement. However, the grain refinement was observed on Cu, Zn, and Brass layers after seven CARB cycles, indicating the positive impact of rolling rotation on obtaining the ultrafine grains. Better ductility of Brass also resulted in finer grains. This also led to more significant improvement in mechanical properties. During the CARB process, the hardness of Zn and Brass increased from 59 HV and 250 HV to 89 HV and 310 HV, respectively.

An Integrated Double-Sided LCC Compensation Based Dual-Frequency Compatible WPT System with Constant-Current Output and ZVS Operation

This article presents an integrated double-sided inductance and double capacitances (DS-LCC) compensation based dual-frequency compatible wireless power transfer (WPT) system. A cascaded single-phase multi-frequency inverter (CSMI) is constructed to generate the independent dual-frequency power transfer signals. In order to achieve the load-independent constant-current output (CCO) at two frequencies, an integrated DS-LCC compensated topology is reconstructed. By configuring the frequency-selective resonating compensation (FSRC) network in the primary side, the power transfer signals at two frequencies can be superimposed into a single transmitting coil, reducing the cost and volume of the system. Furthermore, to implement zero-voltage switching (ZVS) of the CSMI throughout the entire power range, a general parameter design method of the proposed system is also introduced. A 1.5-kW experimental prototype is built to validate the practicability of the presented dual-frequency compatible WPT System. The system can supply power to different loads at two frequencies simultaneously with CCO and ZVS properties. The peak efficiency reaches 91.75% at a 1.2-kW output power.

Obstructed Branching Networks: A Constructal Approach in Fluid Flow Investigation

Tree flow networks are common in both natural and manufactured systems. The organization of the flow hierarchy passes through the dimensional evolution of the form that is linked to the function. Thus, the objective of comparing bifurcated tube networks obtained by the constructal design method, where part of the structure is obstructed, aims to understand the effects on fluid flow and the prediction of evolutionary deviations in its function. This study compares designs of 3D tree networks with various homothety reduction factors for sizes, having tubes obstructed in some locals of the network. In this computational fluid dynamics study, the geometric constraint applied to these networks is the equal total volume of tubes at each branch level. The evaluation is based on the flow resistance of the networks. This study shows, among other things, that the performance of tree designs is highly dependent on geometric characteristics and the branching level where the obstructions are applied. The effect of the number and position of tubes obstructed in the network, as well as the alignment of the tubes across the network branching levels, on the asymmetry of fluid flow through the network is also studied. It is recommended that the results presented be considered when designing networks for engineering systems.

Design and experimental validation of an aspheric multi-lenticular plano-convex lens for VCSEL beam collimation

In this work, we propose a design method of an aspheric lens that achieves collimation for a VCSEL laser beam. The designed lens features a planar front surface and an aspheric back surface of which the profile is mathematically characterized and precisely determined based on the proposed method. The method is derived from a basic geometric-optics analysis and construction approach. The collimating effect of the lens was first analyzed in simulation and then validated in experimental measurement. The experimental results show that the collimator lens transforms the input VCSEL laser beam divergence angle from 25° (436.33 mrad) to an output angle of 3.6906 mrad.

Propagation Effect Analysis of Existing Cracks in Box Girder Bridges Based on the Criterion of Compound Crack Propagation

Cracking in concrete box girder bridges will have a significant impact on the safety and durability of the structure, and many box girder bridges which are in service have undergone varying degrees of cracking. Currently, the safety design of actual bridge projects place an emphasis on the stress or the load value of a cross section at the limit value specified in the code for safety control. This design method assumes that the member itself is of uniform and continuous material and is internally undamaged. However, the bridge structure is more or less cracked to varying degrees during the period from casting to construction to operation of the concrete members. In this paper, a finite element computational model of a three-span prestressed concrete box girder bridge with existing cracks is established based on the fracture mechanics theory, and the critical parameters of crack extension are introduced to evaluate the extension state of cracks. At the same time, the extended stability of the existing cracks of the box girder bridge is analyzed by considering the temperature effect, vehicle loading, and prestressing loss, and the sensitivity of crack extension under each working condition is investigated. The results show that, with the increase in crack length and depth, the crack expansion is promoted, but the effect is relatively small, and the maximum stress intensity factor is only 6.48 MPa mm1/2. Under the multi-factor coupling effect, the cracks show a composite crack expansion dominated by type I cracks, the longitudinal cracks of the existing base plate are in a stable state, the maximum value of the crack expansion critical parameter of the vertical cracks of the webs reaches 1.087, and there is a tendency to expand locally. The maximum value of the critical parameter for crack extension of the vertical crack in the web plate reaches 1.087, and there is a tendency towards local expansion. The crack extension evaluation criteria proposed in this paper have a certain reference value for crack extension research on similar concrete box girder bridges and provide a scientific basis for the optimized design of similar bridges.

Multi-objective parameter design and economic analysis of VSG-controlled hybrid energy systems in islanded grids

Photovoltaic (PV) systems offer cost-effective power solutions for outlying islands but often compromise system stability due to reduced inertia. This study introduces a Virtual Synchronous Generator (VSG) control strategy, integrated with Energy Storage Systems (ESS) and PV, to enhance system inertia. By optimizing coordination between these energy sources, the proposed method mitigates oscillations and improves grid stability. However, PV-VSG systems are generally not favored by energy providers due to the requirement for pre-curtailment of power output. To address this, the paper proposes a parameter design method for VSG control of ESS and PV, utilizing multi-objective genetic algorithm (MOGA) optimization to simultaneously increase the frequency nadir and minimize the settling time after disturbances. Additionally, an adaptive curtailment decision and parameter design method based on artificial neural networks is introduced to enhance the feasibility of PV-VSG systems by reducing PV pre-curtailment and prioritizing PV power release and ESS charging during frequency oscillations. Real data from the Penghu Archipelago in Taiwan are used to build a dynamic model in DIgSILENT, enabling interaction with MOGA. The Value at Risk (VaR) method with dual stochastic variables is employed to assess the allowable PV installed capacity. The results show that when VaR is set at 1%, the proposed PV-VSG method can increase PV penetration by 57.5% compared to scenarios without VSG. Furthermore, compared to traditional PV-VSG methods, the proposed approach achieves a 16.8% increase in PV penetration and reduces annual PV curtailment by 25 MWh. This study also evaluates the economic impact of planners choosing different risk levels, offering valuable insights for grid development in remote or island regions.

An Urban Renewal Design Method Based on Carbon Emissions and Carbon Sink Calculations: A Case Study on an Environmental Improvement Project in the Suzhou Industrial Investment Science and Technology Park

In the process of urban renewal, the high carbon emissions caused by pollution from construction waste and the consumption of materials and energy via “demolition and construction” present significant problems. Calculating carbon emissions and sinks is a prerequisite to carving out a low-carbon path via urban renewal. In this paper, we take an environmental improvement project in the Suzhou Industrial Investment Science and Technology Park as an example. Under the framework of the entire life cycle, we define the “renewal phase”, which includes “demolition” and “construction”, as the calculation boundary, and use the emission coefficient method as the primary calculation approach to assess carbon emissions and sinks. We construct a design-oriented carbon revenue and expenditure estimation system based on the BIM model. After its application in empirical cases, the results show the following: ① The building carbon emissions of Scheme A are 342 t higher than those of Scheme B, due to the fact that the “demolition and construction” works in the former increased by 6000 m2 compared to the latter. ② The landscape carbon emissions of Scheme B are 269 t higher than those of Scheme A, due to the addition of a 2500 m2 reinforced concrete overhead walking platform. ③ The annual carbon sink of Scheme A is six times higher than that of Scheme B, due to the fact that the number of trees and shrubs in the former is five-to-six times greater than in the latter. The number of trees planted plays a decisive role in enhancing the carbon sink benefit. ④ In the year during which the renewal project was implemented, the difference in carbon emissions between Scheme A and Scheme B was 72.9 t. By the 16th year, the difference in carbon emissions between the two schemes approached zero, and the carbon reduction advantages of Scheme A became more pronounced over the entire life cycle thereafter. Finally, this article summarizes four low-carbon design strategies for industrial park renewal projects: rational construction and demolition, three-dimensional parking, low-carbon materials, and plant carbon sequestration. In the context of inventory renewal, this article makes a significant contribution in terms of carbon footprint control and the “dual-carbon” goal.

Integrated design method for protection against vibration of offshore platform plate structure

The paper integrates the modal avoidance method, pedestal design method, and dynamic vibration absorber theory to investigate vibration control technology for offshore platform plate structures. We develop an integrated design method for vibration suppression by controlling the vibration excitation load, vibration transmission, and vibration energy dissipation. Firstly, a modal avoidance design is implemented to suppress vibration transmission along the propagation path of the plate structure. Subsequently, pedestal optimization is conducted for pedestal structure to attenuate vibration excitation at the input end. Finally, dynamic vibration absorber theory is employed to control dominant vibration frequency responses further and achieve multi-target vibration control. Case simulation results demonstrate that the integrated design method reduces the acceleration vibrations level at 113.75, 145.61, and 153 Hz, and this method could guide multi-objective vibration control in offshore platform plate structures.

Experimental and chemical kinetic study on effects of H2-DME fusion addition on laminar premixed flame speed and flame instability for ammonia composite combustion

Improvement of the laminar flame speed and stability for ammonia combustion by using H2 and DME has received significant attention. In this study, the characteristics of NH3/H2, NH3/DME and NH3/H2/DME were investigated in constant-volume bomb with high-speed schlieren technique and chemical kinetics. The results show that DME or H2 addition mainly acts as “start-up" or “acceleration" for ammonia laminar flame speed increasing, respectively. In NH3/H2/DME laminar flame, the increase of DME addition ratio not only increases the intensity but also advances the onset of self-acceleration. At ammonia substitution ratio of 10 %–70 %, the Markstein length of NH3/H2/DME flame are all significantly improved compared with pure ammonia flame. The H2-DME fusion-addition can mitigate the reduction of flame thickness and keep a relatively minor thermal expansion ratio. In NH3/H2/DME laminar flame, DME addition can increase the Lewis number greater than 1.0 at ammonia substitution ratio of 10 %–40 %, which significantly reduce the thermo-mass diffusion instability in comparison with H2 addition. Based on mixture design method and experimental validation, it was found that the flame speed at 473 K, 5 bar and φ = 1 for NH3/H2/DME blending ratios of 53.94 %/26.61 %/19.45 % are greater than those of NH3/H2 and NH3/DME with the same ammonia substitution ratios.

Games4Justice: Course Design Report

Two games were designed as part of an autoethnographic, teacher research study conducted from 2013-2014 on the implementation of play-, game-, and design-based teaching and assessment. The study was conducted at an urban, independent, K-12 day school with fewer than 500 students, respectively, and supported as part of a certification program in Serious Game Design &amp;amp; Research. La Isla de Monstruos was collaboratively designed with students as part of a seventh-grade Spanish 2 &amp;amp; 3 language and culture course using a culture circle design method. Students collaboratively designed a month of gamified and design-based assessment reviewing the material covered during the previous semester. Escapar Tenochtitlan is a six-week anthropology roleplay and language learning game designed by eighth-grade Spanish 2 &amp;amp; 3 students through a culture circle design method, grounded in ethnohistorical, archaeological, and anthropological data of 60 Pre-Columbian sites.

An improved RRT behavioral planning method for robots based on PTM algorithm

For multi-dimensional high-order nonlinear systems with unstable path quality in parameter and extension terms, we developed a new fast search random tree strategy. First, we established a high-order Lipschitz vector field dynamic system to adapt to high-order systems of multi-degree-of-freedom robots, with the complex obstacle function being one of its key components. Secondly, we designed a classification gap filtering network layer (Classification LSTM) to screen training data models and ensure the global stability of data in path design. Additionally, the visual sensors deployed in the unit area effectively implement the path marking backtracking strategy and dead zone path simplification. Finally, three examples are provided to verify the effectiveness of this design method.

Utilization of beef lung powder in model chicken emulsion formulation

AbstractThe present study aimed to demonstrate the effect of beef lung powder addition to model chicken emulsion formulations on quality parameters and determine the optimum usage dose. A mixture design method was employed to determine the ideal proportions of chicken meat, animal fat, and water. The optimal formulation comprises 70.48% chicken meat, 12.42% animal fat, 9.30% water/ice, and 7.80% beef lung powder (w/w). The predicted outcomes for this optimized emulsion include a cooking loss of 3.05%, emulsion stability of 85.21%, an oxidation rate increase of 2.93%, a color difference of 13.24%, and firmness of 24.16 N. The use of lung powder resulted in a reduction in cooking loss and an increase in emulsion stability and hardness. Nevertheless, an increase in both color change and oxidation rate was found in emulsion models. The results of this study demonstrate that beef lung powder is a highly functional ingredient with the capacity to significantly enhance the stability and texture of chicken emulsions. Furthermore, it has the potential to considerably improve the nutritional and quality attributes of emulsified meat products, therefore suggesting its broader applicability in food production.

Theoretical model of ultimate shear capacity and flexural capacity design method of boundary elements for reinforced concrete frames with steel plate shear walls

AbstractThe steel plate shear walls (SPSWs) have been proven effective in reinforced concrete frames (RCFs) as a lateral force‐resistant structure. Despite of advancements, accurately predicting the ultimate shear capacity of RCFs with SPSWs remains challenging using current simplified models. Additionally, the flexural capacity design procedure for the boundary elements (beams and columns) in previous studies of RCF‐SPSWs involved intricate iterative procedures, hindering its widespread implementation. To address the two issues, this paper investigates the pushover responses and the plate‐frame interaction (PFI) of an RCF‐SPSWs system using theoretical and numerical methods. There are three main contributions. First, a theoretical model of ultimate shear capacity for RCF‐SPSWs is proposed, which can also be used to predict shear contributions of boundary frames in RCF‐SPSWs. Calculation errors for ultimate shear capacity of RCF‐SPSWs and shear contribution from the boundary frame are only 3.7% and 6.7% respectively, which are reduced dramatically compared with the traditional model. A simplified schematic diagram for the global collapse mechanism (uniform distribution of plastic hinges within a structure) of RCF‐SPSWs is developed to facilitate the calculation of internal work and reaction forces. Secondly, a flexural capacity design method for the boundary elements to avoid in‐span plastic hinges is proposed. The proposed method enables the achievement of direct estimation of the flexural demands that could trigger a global collapse mechanism, all without intricate iterative procedures. The applicability of current assumptions for the design of steel boundary frame in RCF‐SPSWs system is discussed, and engineering suggestions are provided to ensure safer and more economic designs. Comparison results confirmed the applicability of the proposed design method, which can be adopted to achieve the global collapse mechanism for RCF‐SPSW system. Thirdly, impacts of yielding panel actions on the flexural capacity of boundary elements of RCF‐SPSWs are clarified. Comparison results demonstrated that adding SPSWs to an RCF alters the axial force on boundary elements and significantly impacts the flexural capacity. A design suggestion is made to emphasize the importance of avoiding the balanced failure of boundary elements. The proposed theoretical model can be used to economize the cross‐section of boundary elements in RCF‐SPSWs system under seismic loads due to accurate prediction of their shear contribution; the proposed flexural capacity design method can achieve a global collapse mechanism, and thus the structural safety and energy dissipation capacity are improved; moreover, the building design efficiency is also improved due to avoidance of intricate iterative procedures.