Preservation Methods Research Articles

Advancing Precision Oncology: Whole-Exome Sequencing in Endometrial Cancer Liquid-Based Cytology.

Diagnostic strategies for endometrial cancer have been evolving, with cytologic analysis being considered a key method in integrated oncologic diagnostics because of its less invasive nature and adaptability to various assessments. Liquid-based cytology (LBC) has emerged as a promising method for intact DNA preservation; it exhibits improved efficiency in advanced sequencing applications such as next-generation sequencing. However, despite the use of LBC in panel assays, its application in whole-exome sequencing (WES) for comprehensive genomic profiling remains underexplored. To investigate whether molecular classification is possible based on WES using DNA derived from LBC specimens. We combined WES with targeted gene panel analysis to compare genomic findings of LBC and traditional tissue samples obtained from 7 cases of endometrial cancer. We investigated pathogenic mutations, tumor mutational burden, and microsatellite instability, and achieved molecular classification with high accuracy. We found a substantial concordance between LBC and traditional tissue samples in terms of pathogenic mutation detection, with a 95% match in the LBC samples and 94% in the tissue samples. Notably, our results highlight the importance of combining WES with panel-based analysis in identifying the ultramutated status of a case that had been missed during panel analysis. Our findings emphasize the potential of LBC samples in the precise and noninvasive genomic analysis of cases of endometrial cancer and offer a new avenue for developing diagnostic and therapeutic strategies in precision oncology.

Hyperspectral imaging of human liver allografts for prediction of initial graft function

PurposeIschemia reperfusion injury represents a significant yet difficult to assess risk factor for short- and long-term graft impairment in human liver transplantation (LT). As a non-invasive, non-ionizing tool, hyperspectral imaging (HSI) is capable of correlating optical properties with organ microperfusion. Hence, we here performed a study of human liver allografts assessed by HSI for microperfusion and prediction of initial graft function.MethodsImages of liver parenchyma of 37 human liver allografts were acquired at bench preparation, during normothermic machine perfusion (NMP), if applicable, and after reperfusion in the recipient. A specialized HSI acquisition software computed oxygen saturation (StO2), tissue hemoglobin indices (THI), near infrared perfusion indices (NIR), and tissue water indices (TWI). HSI parameters were analyzed for differences with regard to preservation technique, reperfusion sequence and presence of early allograft dysfunction (EAD).ResultsOrgan preservation was performed by means of NMP (n = 31) or static cold storage (SCS; n = 6). Patients’ demographics, donor characteristics, presence of EAD (NMP 36.7% vs. SCS 50%, p = 0.6582), and HSI parameters were comparable between both groups of preservation method. In organs developing EAD, NIR at 1, 2, and 4 h NMP and after reperfusion in the recipient was significantly lower (1 h NMP: 18.6 [8.6–27.6] vs. 28.3 [22.5–39.4], p = 0.0468; 2 h NMP: 19.4 [8.7–30.4] vs. 37.1 [27.5–44.6], p = 0.0011; 4 h NMP: 26.0 [6.8–37.1] vs. 40.3 [32.3–49.9], p = 0.0080; reperfusion: 13.0 [11.5–34.3] vs. 30.6 [19.3–44.0], p = 0.0212).ConclusionHSI assessment of human liver allografts is feasible during organ preservation and in the recipient. NIR during NMP and after reperfusion might predict the onset of EAD. Larger trials are warranted for assessment of this novel technique in human LT.

Profiles, tissue and microbial integrity of cadavers used in medical faculties in South-western Uganda: implication in anatomical education.

Cadaveric dissection has remained an integral part of anatomical education globally; hence, the profiles of cadavers, their gross tissue and microbial integrity has great implication in anatomical education. This study determined the profiles, gross tissue and microbial integrity of cadavers used in South-western Uganda (SWU) medical schools. A cross-sectional study was carried out for three months in anatomy and microbiology laboratories of SWU medical teaching institutions. Ethical approval was obtained, cadavers were proportionately selected and examined grossly, and surface swabs taken for microbial analysis. Data collected was entered into Microsoft Excel, cleaned, and exported to Stata version 17 for analysis. Majority of cadavers were male (68%), adult (96%), blacks (100%), unknown cause of death (96%), obtained as unclaimed bodies (100%) and with no cadaver record tag (96%). Nearly a half (48%) cadavers had disrupted dentition, 68% had poor muscle integrity and 80% with abnormal fat. About 27% cadavers had poor surface microbial integrity among which 62.5% had a mixture of both bacteria and fungi, 25.0% had only fungi while 12.5% had only bacteria. A high number of cadavers had micro-organisms on their surfaces with majority having a mixture of bacteria and fungi. Majority of cadavers had poor gross tissue integrity. Better cadaver preservation methods should be adopted. To advocate for the establishment of cadaver donation policy and programs through which good quality cadavers can be obtained.

Antibacterial mechanism of atmospheric cold plasma against Pseudomonas fluorescens and Pseudomonas putida and its preservation application on in-packaged red shrimp paste

This study aimed to investigate the antibacterial mechanism of atmospheric cold plasma (ACP) against Pseudomonas fluorescens and Pseudomonas putida and its preservation effect on red shrimp paste. A reactive species (RS) assay showed that O3, H2O2, and total nitric oxide were generated after ACP treatment, which possessed great potential for antibacterial and food preservation. In vitro antibacterial results showed that excess RS inhibited bacterial activity through cell membrane damage. Molecular docking predictions and enzyme activity assays indicated that ACP-induced RS might deactivate dehydrogenases (such as malic dehydrogenase) by oxidatively modifying the active sites. Fluorescence quantification experiments validated the damage of RS to dsDNA. Further preservation tests on shrimp paste demonstrated that ACP treatment significantly delayed the increase in total viable count, Pseudomonas count, and total volatile basic‑nitrogen during refrigeration. This study deepened the understanding of the antibacterial mechanism of ACP and highlighted its potential application as a new preservation method.

Effect of Different Coating Materials on Black Périgord Truffle (Tuber melanosporum) Aroma Profile and Its Shelf Life

AbstractBlack Périgord truffles (Tuber melanosporum) are the highest-priced edible fungus in the world due to their unique flavour, rarity, short growing season, difficulty in mass cultivating, and short shelf-life. The current industrial practices have not been effective in extending truffle shelf-life while preserving its aroma profiles. This study aimed to determine the effectiveness of several preservation methods on Australian-grown black Périgord truffles, which include assessing the changes in the volatile organic compounds (VOCs) of truffles treated with edible coatings, antimicrobial agents, or antioxidants such as chitosan, gum Arabic, kafirin, natamycin, tocopherol, vitamin C, and citric acid at the interval of 0, 7, and 14 days of storage. The study also aimed to assess the capability of gamma-cyclodextrin (γ-CD) in encapsulating truffle VOCs at the intervals of 0, 14, and 28 days of storage. Among all the edible coatings, chitosan-treated truffles had the least change in VOCs, especially the black truffle aroma volatile markers, 2-methyl-1-butanal, 2,4-dithiapentane, and dimethyl sulphide. Chitosan also resulted in no significant changes (P < 0.05) in the carbon dioxide emissions of truffle. The PCA plots showed that chitosan-coated samples displayed the least changes. The sole application of antimicrobial agents or antioxidants was ineffective in delaying the deterioration process. On the other hand, the results show that γ-CD was able to encapsulate 30 truffle’s VOCs, which included 3-methyl-1-butanal, 2-methyl-1-butanol, dimethyl sulphide, and 2,4-dithiapentane with no significant changes over the storage period.

Multielement electrochemical vapor generation system coupled with atomic fluorescence spectrometer for simultaneous measurement of trace levels of As and Hg in coastal seawater samples

BackgroundToxic elements As and Hg in coastal waters affect human health through the food chain even at trace levels. The simultaneous determination of As and Hg in seawater using electrochemical vapor generation (ECVG) with atomic fluorescence spectrometer (AFS) currently has technical bottlenecks. One is the need for a simple and efficient chemical reaction cell and corresponding parameters to generate both AsH3 and Hg vapors, and the other is a suitable preservation method of the mixed standard solutions. ResultsA novel, efficient, online ECVG device was developed and interfaced with a laboratory-built multichannel AFS. The electrolytic cell adopted Sn–Pb alloy as the cathode material to generate both AsH3 and Hg vapors by electrolysis, replacing the unstable reagent tetrahydroborate (THB) usually used in most traditional vapor generation methods. The AFS was equipped with a built-in H2 generator for fuel supply, instead of the conventional acid-THB system. A mixed standard solution of As and Hg was prepared and preserved using thiourea-ascorbic acid as a fixing agent for Hg and a prereduction agent for As(V). The method detection limits (LOD, 3σ) were 0.005 and 0.003 μg/L for As and Hg, respectively. The relative standard deviations (RSD, n = 8) of the spiked samples were 2.8 % (0.50 μg/L As) and 3.1 % (0.05 μg/L Hg), respectively. The recoveries of standard spiked seawater samples with different salinities ranged from 86.1 % to 115.3 %. SignificanceThe system has been successfully applied to the simultaneous analysis of As and Hg in the seawater samples collected from the Xiamen coastal area. The study results provide a sensitive, accurate, and efficient method to promote the development and utilization of simultaneous analysis of multielement in seawaters.

Evaluation of a Sustainable Production of Encapsulated Chili Pepper Powder (Capsicum pubescens) through Convective and Vacuum Drying

Throughout the world, chili and chili powders are spices of considerable economic importance. Rocoto (Capsicum pubescens R. & P.) is a chili pepper used in Andean cuisine. Fresh chili is perishable and therefore it is convenient to protect the capsaicin, color and other bioactive compounds through preservation methods such as encapsulation. The present study systematically evaluates the technical and environmental aspects of producing encapsulated Rocoto red chili powder using three different wall materials—maltodextrin, rice protein and rice flour—in conjunction with two drying methods, namely convection drying and vacuum drying. Our technical objectives focused on developing an encapsulated product with high heat and vibrant red color, key attributes that significantly influence consumer purchasing behavior. In addition, the study aimed to mitigate the loss of polyphenols, flavonoids and antioxidant activity. After identifying the optimal conditions for each wall material and drying process, a comprehensive environmental assessment was conducted to identify the most efficient and sustainable production methods. The results demonstrate that rice flour encapsulation (20%) in conjunction with hot air drying (40 °C) represents the most efficacious method for the preservation of chili powder’s pungency. This approach resulted in a mere 24% reduction in Scoville units while maintaining the powder’s vibrant red coloration. This method not only preserved key bioactive compounds, including capsaicin, polyphenols and flavonoids, but also significantly enhanced the antioxidant capacity of the product. From an environmental perspective, this approach offers significant sustainability benefits, reducing the overall environmental impact by approximately 72% compared to vacuum drying of unencapsulated chili. The use of rice flour as an encapsulant aligns with sustainability goals, making it the most efficient option for balancing product quality and environmental performance.

Participatory Media and Game Preservation: A Taxonomy of YouTube Game‐Related Content

ABSTRACTVideo games are both information objects as well as experiences of play. However, current methods of game preservation often fail to capture the full gaming experience. To address this, one proposed solution involves leveraging player‐generated content as an alternative to emulation. To investigate the viability of this suggestion, we examine the following research questions: (1) What types of gameplay content exist on the platform YouTube? and (2) What are the challenges and opportunities in utilizing this style of community created content for game preservation? We introduce a Taxonomy of YouTube Game‐Related Content to answer these questions and discuss its application, along with the challenges and opportunities that arose during its creation. Our taxonomy and findings suggest that although utilizing this content for game preservation requires additional knowledge of games and their community, as well as consideration of how the platform and creators impact game history, it also presents opportunities to preserve more diverse voices and experiences of play. Furthermore, it suggests YouTube videos offer a potential avenue, not only for preserving the game object, but also for preserving its broader cultural significance and multiple forms of knowledge.

Freezability of Semen Collected from Ganders of 12 Breeds Covered by the Polish Genetic Resources Conservation Program

Abstract A rapid decline in wild animal biodiversity, as well as in the number of local livestock breeds of lower economical values, makes species and breeds protection and creation of genetic reserves a necessity and a challenge of the 21st century. For birds, semen freezing is still the best method of gene preservation ex situ in vitro. The aim of the presented study was to assess the susceptibility to freezing process and to establish the semen gene bank of twelve goose breeds covered by the Polish genetic resources conservation program. Sexually mature ganders were randomly selected from four northern local breeds: Kartuska, Pomorska, Rypinska, Suwalska, four southern local breeds: Garbonosa, Kielecka, Lubelska, Podkarpacka, and four foreign goose breeds: Kuban, Landes, Roman and Slovakian. Each breed was represented by 12 males, twelve repetitions were carried out. Semen samples were frozen in a computer-controlled cryogenic chamber using a method developed for White Koluda goose. The basic evaluation criterion was sperm morphology in fresh and freeze-thawed semen assessed in nigrosine-eosin stained histological smears. The percentage of live in total and live normal sperm in the fresh semen differed significantly (P<0.05) between breeds. Semen of Kartuska and Suwalska ganders (northern breeds), contained the highest percent of live sperm – 94.4 and 94.1 respectively, including 52.5% and 54.2% of live normal sperm, while the lowest number of these forms was observed for Slovakian goose – 86.4% and 29.2%, respectively. The freezing process resulted in a significant (P<0.05) reduction in the total number of live and live normal sperm and an increase in dead sperm in all groups. Cryopreservation process was the most effective in case of Suwalska goose semen (28.4% of live normal cells in thawed semen) and the less profitable for Landes (7.4% of live normal cells). The analyses showed significant differences between breeds in sperm morphology in fresh and thawed semen. Moreover, the high proportion of live spermatozoa in the fresh semen did not guarantee a high proportion of spermatozoa after thawing, indicating the differences in semen susceptibility to cryopreservation process depending on goose breed.

A Comprehensive Analysis of Apricot Drying Methods via Multi‐Criteria Decision Making Techniques

ABSTRACTFood and food safety have been among the most important issues for people throughout history. Societies have always tried to be self‐sufficient in food and have avoided becoming dependent on foreign sources. However, the fact that most foods are seasonal and the increasing population's food consumption have revealed the need to preserve foodstuffs for a long time. The old and well‐known method used today for extending shelf life is the drying process. The drying process is preferred over other preservation methods for reasons such as being more economical, easier to transport, having a longer shelf life, more concentrated nutritional value, and containing fewer additives. This ensures that dried foods are of higher quality in terms of physical, chemical, microbial properties, and nutritional values compared to other packaged foods. While the drying process was traditionally done over a long period, technological advancements have led to the production of higher quality and more valuable commercial products in a shorter time. In this study, traditional and technological methods used in drying apricots were compared according to the parameters determined by experts in the field. Since multiple parameters are effective in the comparison, Multi‐Criteria Decision Making (MCDM) techniques were used. The optimum apricot drying method was determined by combining the results obtained from different MCDM techniques with the Borda rule.

Factors influencing wildmeat trade in Guyana and expected changes in the context of the oil-related development prospects

The recent offshore oil discovery in the Guiana Shield is expected to bring about significant changes to the area, such as increased GDP per capita, infrastructure development, and urbanization. The potential impact on the wild meat trade depends on factors influencing its demand and provision. Through interviews and group discussions with trade chain stakeholders in all towns of Guyana, we evaluate wildmeat trade sector and explored predicted changes on it in 2033 with the prospects for short term oil-related development. The most traded species in Guyana included paca, white-lipped peccary, deer, tapir and capybara and a total of 38.46 % (5 out of 13) of the taxa being traded is classified as threatened of extinction. Regions with higher population size and GDP per capita, are the main trade hubs for wildmeat. Access to improved preservation methods (e.g. freezers) and motorized transportation options (eg.: boat with engines and vehicles) significantly influence higher volumes of wildmeat traded. The economic growth anticipated in Guyana is expected to boost population growth and, by the same time, wildmeat demand in urban areas. Concomitantly, with improved infrastructure and increased access to electricity, wildmeat provision will be facilitated across a wider catchment area. Based on the assumption that cultural patterns shaping wildmeat demand and environmental regulations will likely not change at the same rapid path as economic growth in the next ten years, we predict wildmeat trade volumes to increase to 10,280 tons/year by 2033. We identify three main opportunities to ensure a sustainable wildmeat sector in the context of the economic boom: First, the sector requires to be well regulated through a licensing and a quota system that can be adequately enforced. Second, efforts to curve demand on the Coast need to be strengthened based on well designed and culturally adapted behaviour change campaigns. Third, local communities and indigenous people need to be empowered to protect and conserve their territories and wildlife resources, in particular with the authority to exclude illegal hunters.

Drying kinetics and mathematical modelling of coconut meat slices: Insight into lyophilization and oven drying synergic effect

Coconut consumption provides nutritional and health benefits to humans. Yet, poor postharvest and preservation methods result in the loss of coconut fruit bunches yearly. Investigations into achieving drying techniques that result in optimum drying rates coupled with consumer’s desired end products that are both commercially and nutritionally viable remain paramount in the food industry. Consequently, osmotic dehydration is commendable for its low-cost pretreatment merit. Therefore, the study examined the drying-kinetics, energy consumption, effective moisture diffusivity (Deff), vitamin C retention, color, and rehydration behavior of coconut cultivars (Sri Lanka Green Dwarf×Vanuatu Tall (SGD×VTT), Catigan (CAT), and Tacunan Green Dwarf (TGD)) meat slices. Samples were osmotically pretreated with sucrose solution (30%/30 min) and subjected to oven drying (80, 90, 100, and 110 °C (air velocity of 2 m/s) and lyophilization (-45 ± 2 °C) methods. A mathematical model was employed to predict the effect of osmotic pretreatment on drying dynamics and the assessment of utilized energy, vitamin C content, color, and rehydration of coconut meat slices at different drying conditions. The oven-dried sample’s color changed (p<0.05) compared to lyophilized samples. Drying-kinetics models were validated using determination coefficient (R2) and root mean square error (RMSE). The Asymptotic model suited the samples’ drying data goodness fitting based on R2 and low RMSE compared to other models for both drying methods. Deff ranged between 1.10×10-07 m2s-1 and 7.90×10-08 m2s-1 for all the drying methods. CAT sample retained high vitamin C content compared to SGD×VTT and TGD samples. Rehydration ratio values were significant at lower temperatures among oven-dried experimental samples whereas TGD samples exhibited a significant rehydration values compared to SGD×VTT and CAT lyophilized samples. Among the drying methods, oven drying exhibited low energy consumption with shorter drying time and optimum Deff per adopted temperature ranges compared to lyophilization. The study revealed that temperature, time, the sucrose solution, and the thickness of the coconut slices strongly influenced the drying kinetics of the osmotically pre-treated coconut meat slice’s drying features.

Hadith Preservation: Techniques And Contemporary Efforts

Hadith is an important source of Islamic teachings. The method of Hadith preservation during the time of Prophet Muhammad (peace be upon him) and after his demise has constantly been criticised by Orientalists and critics of Islam. Most of their objections emphasise that Hadith could not have been accurately transmitted orally over a century. Thus, conducting research on how Hadith has been preserved is crucial, particularly to remind the critics that Allah’s (Subhanahu Wa Ta’ala) promise to protect His Book is not limited to the Qur’an, but also extends to the Hadith, which explains the meanings of the Qur’an. Additionally, it is crucial to continuously discuss the painstaking efforts that went into Hadith preservation, which make Muslims believe with absolute certainty that Hadith were accurately preserved. On this basis, this article discusses the multifaceted landscape of Hadith preservation by exploring the techniques employed and contemporary efforts in preserving this rich Islamic heritage. Relevant books, academic journal articles, lecture notes, and webpages were reviewed in writing this article. The three main techniques that played a significant role in preserving Hadith are memorisation, the principle of practice, and documentation. Moreover, the contemporary efforts that contributed to Hadith preservation include the massive publication of academic books and journals on Hadith, the establishment of faculties in major universities that offer Hadith studies as a specialised academic program, the increase in comparative jurisprudence studies that refer to Hadith as one of the most important references, the need to respond to Orientalist theories on Hadith, and the advent of digital technology.

Assessment of the impact of locally fabricated oven and sun drying method on the proximate, mineral and microbiological composition of Clarias gariepinus (catfish)

Drying is an age long practice which prevent fish from deterioration and spoilage, however, traditional drying is usually practiced in rural settings with poor hygienic conditions, thus, the nutritional and microbial quality of such products is uncertain. Therefore, the present study was aimed at evaluating the impact of a locally fabricated oven and sun drying method on the proximate, mineral and microbial composition of Clarias gariepinus (Catfish) using standard laboratory procedures. The results of the proximate analysis showed that the oven dried fish had the highest crude protein content (66.43 ± 3.37 %), crude lipid content (8.27 ± 0.28 %) and carbohydrate content (4.50 ± 0.22 %) which were statistically significant (p&lt;0.05) when compared to the sun dried fish, while the sun dried fish had the highest ash content (16.30 ± 2.48 %) and moisture content (14.30 ± 0.82 %) which were statistically significant (p&lt;0.05) when compared to the oven dried fish. Also, the mineral analysis showed that the oven dried fish had the highest calcium (821.49 ± 1.10 mg/kg), phosphorus (86.04 ± 0.83 mg/kg), potassium (254.72 ± 0.40 mg/kg), iron (40.73 ± 0.23 mg/kg), manganese (0.95 ± 0.01 mg/kg) and selenium (14.15 ± 0.39 mg/kg) content which were statistically significant (p&lt;0.05) when compared to the sun dried fish, while the sun dried fish had the highest sodium (173.52 ± 0.12 mg/kg), cupper (0.87 ± 0.12 mg/kg), magnesium (39.01 ± 0.43 mg/kg) and zinc (2.83 ± 0.23 mg/kg) content which were also statistically significant (p&lt;0.05) when compared to the oven dried fish. The study also showed that the total viable bacterial count was higher in the sun dried fish (6.23 x 105 cfu/g) which was not statistically significant (p&lt;0.05) when compared to the oven dried fish (5.65 x 105cfu/g), while the fungal count was higher in the oven dried fish (6.55 x 105 cfu/g) which was also not statistically significant (p&lt;0.05) when compared to the sun dried fish (5.01 x 105 cfu/g). The findings in this study showed that the locally fabricated oven was remarkably effective, yielding fish with significantly enhanced nutritional profiles and minimal microbial contamination. This approach offers a superior method for fish preservation, providing a promising alternative for improving food safety and nutritional quality.

Effects of Efficient Ethylene Remover on the Lignification of Fresh Faba Bean (Vicia faba L.) during Storage.

Postharvest ethylene accumulation and lignification are significant issues affecting the storage quality of fresh faba beans, resulting in rapid quality decline. However, there is still a lack of effective preservation methods to preserve the quality of faba beans during storage. This study aimed to investigate the regulation of lignification in faba beans during storage using a high-efficiency ethylene remover (HEER), examining physiological responses, key enzyme activities, and transcriptomic changes. Results showed that the HEER treatment inhibited the lignification, reducing it by 45% and lowering the respiratory rate of fresh pods by 32.8% during storage. Additionally, the HEER treatment suppressed respiration rates and the activities of lignin synthesis-related enzymes, including phenylalanine ammonia-lyase (from 353.73 to 246.60 U/g), cinnamic acid-4-hydroxylase (from 635.86 to 125.00 U/g), 4-coumarate: coenzyme A ligase (from 1008.57 to 516.52 U/g), and cinnamyl-alcohol dehydrogenase (from 129.42 to 37.12 U/g), thus slowing lignin accumulation. During storage, the hardness of fresh faba bean increased by 9.79% from the initial period, being 1.44 times higher than that of HEER. On days 8 and 16 of storage, the respiratory rate of the treated beans decreased by 24.38% and 4.12%, respectively. Physiological and enzyme activity analyses indicated that HEER treatment-induced increase in hardness was associated with the phenylpropanoid metabolic pathway. Moreover, the HEER significantly down-regulated the expression of several key genes, namely FaPAL, FaC4H, and FaCAD. This study helps to deepen the understanding of the inhibition of lignification by HEER and provide new insights for the development of preservation technology of faba bean.

Cyanoacrylate versus Collagen Membrane as a Sealing for Alveolar Ridge Preservation: A Randomized Clinical Trial.

This study involved a randomized clinical trial that included 140 patients. Alveolar ridge preservation was performed with xenografts. Sealing in the control group consisted of a collagen membrane versus cyanoacrylate in the test group. The dental implants were placed immediately after extraction. The variables were evaluated at 3, 12, and 18 months of follow-up. Pearson's chi-squared test was used for qualitative variables and the Student t-test for related samples was used for quantitative variables. The change in buccolingual alveolar bone width was significantly greater in the CMX group than in the CX group after three months (p < 0.005). However, significance was not reached at the other follow-up timepoints (p > 0.005). CAL showed significantly greater values in the CMX group than in the CX group (p < 0.005), and MBL proved greater in the CMX group than in the CX group, with p < 0.001. Five membrane exposures were recorded in the CMX group. Cyanoacrylate as a sealing method for alveolar ridge preservation seems to afford better clinical and radiological results than collagen membrane.

Biostasis: A Roadmap for Research in Preservation and Potential Revival of Humans.

Human biostasis, the preservation of a human when all other contemporary options for extension of quality life are exhausted, offers the speculative potential for survival via continuation of life in the future. While provably reversible preservation, also known as suspended animation, is not yet possible for humans, the primary justification for contemporary biostasis is the preservation of the brain, which is broadly considered the seat of memories, personality, and identity. By preserving the information contained within the brain's structures, it may be possible to resuscitate a healthy whole individual using advanced future technologies. There are numerous challenges in biostasis, including inadequacies in current preservation techniques, methods to evaluate the quality of preservation, and potential future revival technologies. In this report, we describe a roadmap that attempts to delineate research directions that could improve the field of biostasis, focusing on optimizing preservation protocols and establishing metrics for querying preservation quality, as well as pre- and post-cardiac arrest factors, stabilization strategies, and methods for long-term preservation. We acknowledge the highly theoretical nature of future revival technologies and the importance of achieving high-fidelity brain preservation to maximize the potential of future repair technologies. We plan to update the research roadmap biennially. Our goal is to encourage multidisciplinary communication and collaboration in this field.

Evolution of bacterial flora in raw yak milk and its effect on quality characteristics during frozen storage

Due to the scarcity of yak milk in Tibetan areas, local herders primarily use traditional freezing methods for preservation. However, the extent of microbial contamination in raw yak milk during frozen storage and its impact on milk quality remains largely unexplored. This study analyzed the changes in bacterial populations and quality of raw yak milk stored at −18 °C for 6 months. The results indicated that, compared to fresh milk, the freshness of yak milk showed no significant change after 2 months of frozen storage (P > 0.05). After 4 months, the bacterial diversity of flora in yak milk increased significantly, with Pseudomonas and Acinetobacter emerging as the dominant psychrophilic bacteria. Correspondingly, the expression of metabolic pathways related to quality deterioration increased, with a significant increase in physicochemical indicators such as acidity, proteolysis degree, acid value and fat oxidation degree (P < 0.05). Extending the frozen storage to 6 months resulted in a slight but no significant decrease in bacterial diversity, with no significant difference in quality deterioration compared to the 4-month group (P > 0.05). Correlation analysis further revealed that Acinetobacter and Psychrobacter were significantly positively correlated with quality deterioration of frozen milk (P < 0.05). In summary, when frozen for more than 4 months, raw yak milk exhibited the highest bacterial diversity and predicted the most abundant metabolic pathways, with dominant psychrophilic bacteria massive proliferation leading to a decline in quality and safety.

A Current Trend in Efficient Biopolymer Coatings for Edible Fruits to Enhance Shelf Life.

In recent years, biopolymer coatings have emerged as an effective approach for extending the shelf life of edible fruits. The invention of biopolymer coverings has emerged as an innovation for extending fruit shelf life. Natural polymers, like chitosan, alginate, and pectin, are used to create these surfaces, which have several uses, including creating a barrier that prevents water evaporation, the spread of living microbes, and respiratory movement. These biopolymer coatings' primary benefits are their environmental friendliness and lack of damage. This study highlights the advancements made in the creation and usage of biopolymer coatings, highlighting how well they preserve fruit quality, reduce post-harvest losses, and satisfy consumer demand for natural preservation methods. This study discusses the usefulness of the biopolymer coating in terms of preserving fruit quality, reducing waste, and extending the product's shelf life. Biopolymer coatings' potential as a sustainable solution for synthetic preservatives in the fruit sector is highlighted as are formulation process advances that combine natural ingredients and environmental implications. This essay focuses on the essential methods, such as new natural additives, as well as the environmental effect of biopolymer coatings, which are safe and healthy commercial alternatives.

Normothermic ex vivo kidney perfusion preserves mitochondrial and graft function after warm ischemia and is further enhanced by AP39

We previously reported that normothermic ex vivo kidney perfusion (NEVKP) is superior in terms of organ protection compared to static cold storage (SCS), which is still the standard method of organ preservation, but the mechanisms are incompletely understood. We used a large animal kidney autotransplant model to evaluate mitochondrial function during organ preservation and after kidney transplantation, utilizing live cells extracted from fresh kidney tissue. Male porcine kidneys stored under normothermic perfusion showed preserved mitochondrial function and higher ATP levels compared to kidneys stored at 4 °C (SCS). Mitochondrial respiration and ATP levels were further enhanced when AP39, a mitochondria-targeted hydrogen sulfide donor, was administered during warm perfusion. Correspondingly, the combination of NEVKP and AP39 was associated with decreased oxidative stress and inflammation, and with improved graft function after transplantation. In conclusion, our findings suggest that the organ-protective effects of normothermic perfusion are mediated by maintenance of mitochondrial function and enhanced by AP39 administration. Activation of mitochondrial function through the combination of AP39 and normothermic perfusion could represent a new therapeutic strategy for long-term renal preservation.